DEPARTMENT OF ZOOLOGY



Researcher : Chan DKO
Project Title:Development of video programmes for teaching field courses at KARC
Investigator(s):Prof. Chan D.K.O.
Source(s) of Funding:Run Run Shaw Research and Teaching Endowment Fund
Start Date:February 1991
Abstract:
To develop video programmes for teaching field courses at KARC.


Project Title:Food processing for fermented products
Investigator(s):Prof. Chan D.K.O.
Source(s) of Funding:Private donation
Start Date:July 1996
Abstract:
To modernize fermentation processes for soy fermentation products.


Project Title:Partition of function between the chloride cell and the pavement cell of the gill epithelium of the eel, Anguilla japonica
Investigator(s):Prof. Chan D.K.O.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1997
Completion Date:August 2000
Abstract:
To define the functional roles of the chloride cells and the pavement cells in the overall physiology-biochemistry of the eel gill epithelium.


Project Title:Molecular cloning and expression of a cDNA encoding the stanniocalcin receptor
Investigator(s):Prof. Chan D.K.O. , Dr. Wong C.K.C.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1998
Abstract:
To definitively define the sites of action of Stanniocalcin (STC) hormone, which has been assumed for a century to be unique to fish but has recently been identified in mammalian tissue as well. The main action of this hormone is on calcium and inorganic phosphate metabolism. Its site of action has been found to include the chloride cell of the gill epithelium, the kidney tubules, and possibly the bone.


Project Title:Development of an industrial process to utilize marine mammal oils in fortified foods and functional foods
Investigator(s):Prof. Chan D.K.O. , Dr. Li E.T.S., Prof. Lie Ken Jie M.S.F., Dr. Wan J.M.F., Dr. Xu R.J.
Source(s) of Funding:Industrial Support Fund, Industry Department of Hong Kong Government
Start Date:July 1999
Abstract:
To develop an industrial scale process to concentrate nutritionally valuable components in marine mammal oil, free from undesirable contaminants, and stabilize the preparation, including prevention of autooxidation to toxic metabolites and off flavours, for use in fortified foods and health foods; to provide a firm scientific basis to support the claim that the fortified food product we develop actually effectively delivers the PUFA to the consumer and hence provide the health benefits as claimed.


List of Research Outputs

Chan D.K.O., Xi Z.Q., Selective breeding of native Chinese chickens for meat quality, China-EU Workshop on Animal Biotechnology, Shenzhen 10-13 October. 2000.
Ding H.B., Xu R.J., Chan D.K.O., Meat quality comparison between broilers and Chinese local chickens, Animal Husbandry and Veterinary Medicine. 2000, 32: 16-18.
Lee K.Y., Ko K.W., Chan D.K.O., Dong K.W., Yu K.L., Cloning and characterization of A gonadotropin-releasing hormone receptor in the seabream (Mylio macrocephalus), 82nd Annual Meeting of Endocrine Society, Toronto, Canada. 2000.
Wong C.K.C., Yeung H.Y., Mak N.K., Dimattia G.E., Chan D.K.O., Wagner G.F., Effects of Dibutyryl cAMP on Stanniocalcin and Stanniocalcin-Related Protein mRNA Expression in Neuroblastoma Cells, 82nd Annual Meeting of Endocrine Society, Toronto, Canada. 2000.
Wong C.K.C., Chan D.K.O., Effects of cortisol on chloride cells in the gill epithelium of Japanese eel, Anguilla japonica, Journal of Endocrinology. 2001, 168: 185-192.
Zhang X., Leung F.C.C., Chan D.K.O., Yang G., Wu C., Genetic diversity of Chinese native chickens, China-EU Workshop on Animal Biotechnology, Shenzhen 10-13 October. 2000.


Researcher : Chan MN
List of Research Outputs

Ip S., Chan M.N., Leung F.C.C., Characterization of the chicken genomic growth hormone gene (Wai Chow strain)., Poultry Science. 2000, 79(supp. I): 10.


Researcher : Chan SK
List of Research Outputs

Chan S.K., Xu R.J., Determination of transforming growth factor-b1 and TGF-b2 in porcine colostrum and milk, Proceedings of the Nutrition Society of Australia. 2000, 24: 253.


Researcher : Chan SM
Project Title:Analysis of the crustacean hyperglycemic hormone-like gene of the shrimp Metapenaeus ensis
Investigator(s):Dr. Chan S.M.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Completion Date:June 2001
Abstract:
To study the expression of the cHH-like gene during reproductive cycle of the females and male shrimp; to identify the potential transcription factor binding sites for the cHH-like gene; to identify important regions on the 5' flanking region of the cHH-like gene.


Project Title:The study of shrimp CHH/MIH/GIH gene family: gene structure, expression and biological assay of recombinant neuropeptides
Investigator(s):Dr. Chan S.M. , Prof. Chu K.H.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:February 2000
Abstract:
To study the eyetalk neurohormone genes belonging to the CHH/MIH/GIH family in the shrimp. The long-term goal is to elucideate the hormonal mechanisms regulating growth and reproduction in the shrimp for applications in shrimp farming.


Project Title:The study of shrimp CHH/MIH/GIH gene family: gene structure, expression and biological assay of recombinant neuropeptides
Investigator(s):Dr. Chan S.M. , Prof. Chu K.H.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:February 2000
Abstract:
To study the eyetalk neurohormone genes belonging to the CHH/MIH/GIH family in the shrimp. The long-term goal is to elucideate the hormonal mechanisms regulating growth and reproduction in the shrimp for applications in shrimp farming.


List of Research Outputs

Gu P.L., Chu K.H., Chan S.M., Bacterial expression of the shrimp molt-inhibiting hormone (MIH): antibody production, immunocytochemical study and biological assay, Cell Tissue Research. 2001, 303: 129-136.
He J.G., Wang S.P., Zeng K., Huang Z.J., Chan S.M., Systemic disease caused by an iridovirus-like agent in cultured mandarinfish, Siniperca chuatsi (Basilewsky), in China, Journal of Fish Diseases. 2000, 23: 219-222.
Silva Gunawardene Y.I.N., Chow B.K.C., He J.G., Chan S.M., The shrimp FAMeT cDNA is encoded for a putative enzyme involved in the methylfarnesoate (MF) biosynthetic pathway and is temporally expressed in the eyestalk of different sexes, Insect Biochemistry and Molecular Biology. 2001, 31: 1115-1124.


Researcher : Chow BKC
Project Title:Development of a recombinant vasoactive intestinal peptide (VIP) vaccine to boost the egg-laying performance of domestic geese
Investigator(s):Dr. Chow B.K.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Completion Date:June 2001
Abstract:
To increase the reproductive efficiency of domestic geese by the development of an inexpensive VIP vaccine using recombinant DNA and protein engineering technology.


Project Title:Regulation of the human GnRH receptor gene expression by steriodogenic factor-1 and cAMP dependent signal transduction pathway
Investigator(s):Dr. Chow B.K.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Completion Date:June 2001
Abstract:
To understand the complex interaction of GnRH receptor and its ligand in controlling the systemic and coordinated processes of gonadotropins secretion.


Project Title:The concerted hypophysiotropic actions of GHRH and PACAP on the control of growth hormone release in goldfish, Carassius auratus
Investigator(s):Dr. Chow B.K.C. , Dr. Wong A.O.L.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1999
Abstract:
(1) Hypophysiotropic actions of GHRHcatfish-like and GHRHcarp-like peptides in goldfish pituitary. To demonstrate the presence of both forms of GHRH transcripts in goldfish pituitary and hypothalamus; to identify cells producing GHRHcatfish-like and GHRHcarp-like peptides in goldfish pituitary and hypothalamus by immunostaining techniques; to study the regulation of GH release by these peptides at the pituitary cell level; to clone and to characterize GHRHcatfish-like and GHRHcarp-like receptors; to colocalize GHRHcatfish-like and GHRHcarp-like receptor(s) with somatotrophs and other cell types in goldfish pituitary. (2) PACAP as a novel growth hormone releasing factor in goldfish. To identify PACAP cell-bodies and nerve fibers in goldfish pituitary, hypothalamus and other regions of the brain by immunostaining; to clone and to functionally and pharmacologically characterize PACAP receptors (PACAP type 1, VIP1 and VIP2 receptors); to demonstrate the expression of PACAP receptors in goldfish pituitary and to colocalize these receptors with various cell types. (3) Concerted actions of PACAP and GHRHs in the stimulation of growth hormone release from somatotrophs. To investigate complementary actions PACAP and GHRHs in stimulating GH release.


Project Title:Using cytosensor microphysiometry as a mean to screen functional GnRH agonists and antagonists
Investigator(s):Dr. Chow B.K.C.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:September 2000
Completion Date:June 2001
Abstract:
To assess the real-time responses of GnRH in a gonadotrope-specific cell-model, alpha T3-1; to compare the relative efficacy and potency of commercially available GnRH analogues; to establish a standard test for monitoring Chinese medicines or functiona l foods claimed to have GnRH-like activities; to screen the potential natural and synthetic GnRH analogues.


Project Title:Comparative analysis of the VPAC2 receptors: functional evolution of VIP, PACAP and PHI in vertebrates
Investigator(s):Dr. Chow B.K.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:November 2000
Abstract:
To clone and identify the VPAC2R from a local frog species, Rana tigrina rugulosa; to study the complimentary distribution of frog VPAC1R and VPAC2R by reverse transcriptase-polymerase chain reaction (RT-PCR).


List of Research Outputs

Cheng K.W., Chow B.K.C., Leung P.C.K., Functional Mapping of a Placenta-Specific Upstream Promoter for Human Gonadotropin-Releasing Hormone Receptor Gene, Endocrinology. 2001, 142: 1506-1516.
Cheng K.W., Ngan S.W., Kang S.K., Chow B.K.C., Leung C.K., Transcriptional Down-Regulation of Human Gonadotropin-Releasing Hormone (GnRH) Receptor Gene by GnRH: Role of Protein Kinase C and Activating Protein 1, Endocrinology. 2000, 141: 3611-3622.
Chow B.K.C., Tse L.Y., Pang T.K.R., Wong A.O.L., Identification of a novel PHI receptor in goldfish: Implication on the evoluation of VIP/PACAP receptors in vertebrates., 20th Conference of European Comparative Endocrinologists. 2000, #8S: 88.
Ng S.M., Yu K.L., Yung W.H., Chow B.K.C., Real-Time Analysis of the Activities of GnRH and GnRH Analogs in aT3-1 Cells by the Cytosensor Microphysiometer, Journal of Cellular Biochemistry. 2001, 80: 304-312.
Ngan E.S.W., Leung P.C.K., Chow B.K.C., Interplay of pituitary adenylate cyclase-activating polypeptide with a silencer element to regulate the upstream promoter of the human gonadotropin-releasing hormone receptor gene, Molecular and Cellular Endocrinology. 2001, 176: 135-144.
Silva Gunawardene Y.I.N., Chow B.K.C., He J.G., Chan S.M., The shrimp FAMeT cDNA is encoded for a putative enzyme involved in the methylfarnesoate (MF) biosynthetic pathway and is temporally expressed in the eyestalk of different sexes, Insect Biochemistry and Molecular Biology. 2001, 31: 1115-1124.
Tye A.J., Ghebrehiwet B., Guo N., Sastry K.N., Chow B.K.C., Peerschke E.I.B., Lim B.L., The Human gC1qR/p32 Gene, C1qBP, Journal of Biological Chemistry. 2001, 276: 17069-17075.
Wong A.O.L., Li W.S., Lee K.Y., Leung M.Y., Tse L.Y., Chow B.K.C., Lin H.R., Chang J.P., Pituitary adenylate cyclase activating polypeptide as a novel hypophysiotropic factor in fish., Biochemistry Cell Biology. 2000, 78: 329-343.


Researcher : Chung YYY
List of Research Outputs

Mak C., Chung Y.Y.Y., Ko R.C.C., Single-stranded endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., Parasitology. 2000, 120: 527-533.


Researcher : Ip CY
List of Research Outputs

Ip C.Y., Zhang X.Q., Leung F.C.C., Genomic Growth Hormone Gene Polymorphisms in Native Chinese Chickens, Proceedings of the Society of Experimental Biology and Medicine. 2001, 226: 458-462.


Researcher : Ko KW
List of Research Outputs

Lee K.Y., Ko K.W., Chan D.K.O., Dong K.W., Yu K.L., Cloning and characterization of A gonadotropin-releasing hormone receptor in the seabream (Mylio macrocephalus), 82nd Annual Meeting of Endocrine Society, Toronto, Canada. 2000.


Researcher : Ko RCC
Project Title:Mechanism of a parasite induced mammalian muscle transformation
Investigator(s):Prof. Ko R.C.C. , Dr. Mak C.H.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Completion Date:March 2001
Abstract:
To identify and characterize the nuclear factors in the Trichinella E/S products and to study their roles in muscle transformation; to identify the host target genes involved in the transformation.


Project Title:Molecular characterization of differentially expressed genes in Trichinella spiralis
Investigator(s):Prof. Ko R.C.C. , Dr. Mak C.H.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To identify and characterize the T. spiralis specific transcripts and to study its temporal and spatial expression; to identify potential targets for the diagnosis and treatment of T. spiralis.


Project Title:Characterization of endonucleases in the excretory/secretory products of Trichinella spiralis, a pathogen of man and animals
Investigator(s):Prof. Ko R.C.C. , Dr. Mak C.H.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:February 2000
Completion Date:January 2001
Abstract:
To characterize a novel double-stranded endonuclease secreted by an intracellular parasitic nematode which can affect host muscle cells functions; to purify, clone and sequence the endonuclease.


Project Title:Cloning and sequencing of the metallo-and cysteine proteinases of an intracellular parasite, Trichinella spiralis
Investigator(s):Prof. Ko R.C.C. , Dr. Mak C.H.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:February 2001
Abstract:
To clone and sequence the metallo- and cysteine proteinases of T. spiralis; to study the feasibility of using these proteins as antigens in serodiagnosis.


List of Research Outputs

Ko R.C.C., An update on the immunodiagnosis of food-borne parasitic diseases, 7th Western Pacific Congress of Chemotherapy & Infectious Diseases, Hong Kong. 2000, 102.
Ko R.C.C., Emerging and re-emerging parasitic diseases: new frontiers and challenges in the 21st century, 2nd International Conference on Parasitology and Tropical Medicine, Kuala Lumpur, Malaysia, Oct.. 2001.
Li C.K.F., Ko R.C.C., Inflammatory response in muscles during concurrent infections of Trichinella spiralis and T. pseudospiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 99.
Li C.K.F., Ko R.C.C., The detection and occurence of circulating antigens of Trichinella spiralis during worm development., Parasitology Research. 2001, 87: 155-162.
Lun H.M., Lai Y.Y., Mak C., Ko R.C.C., Identification and molecular cloning of metalloprotease in the excretory/secretory products of the infective-stage larvae of Trichinella spiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 40.
Mak C., Ko R.C.C., Characterization of endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 27.
Mak C., Chung Y.Y.Y., Ko R.C.C., Single-stranded endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., Parasitology. 2000, 120: 527-533.
Wong C.S., Mak C., Ko R.C.C., Single-stranded endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 41.
Wu W.K., Sun C.K., Mak C., Ko R.C.C., Characterization of genes differentially expressed in Trichinella spiralis by heat shock treatment using suppression subtractive hybridization., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 38.


Researcher : Lee KY
List of Research Outputs

Lee K.Y., Ko K.W., Chan D.K.O., Dong K.W., Yu K.L., Cloning and characterization of A gonadotropin-releasing hormone receptor in the seabream (Mylio macrocephalus), 82nd Annual Meeting of Endocrine Society, Toronto, Canada. 2000.
Lee K.Y., Chan P.H., Au T.K., Leung P.C., Wong A.O.L., Goldfish calmodulin: Molecular cloning, tissue distribution, and regulation of its mRNA expression in goldfish pituitary cells., 4th International Symposium on Fish Endocrinology. 2000, 80.
Lee K.Y., Chan V.C.C., Chang J.P., Yunker W.K., Wong A.O.L., Norepinephrine Regulation of Growth Hormone Release from Goldfish Pituitary Cells. I. Involvement of a2 Adrenoreceptor and interactions with Dopamine and Salmon Gonadotropin-Releasing Hormone, Journal of Neuroendocrinology. 2000, 12: 311-322.
Li W.S., Leung R.C.Y., Lee K.Y., Lin H.R., Wong A.O.L., Pituitary adenylate cyclase-activating polypeptide stimulates growth hormone release and synthesis in grass carp pituitary cells., 82nd Annual Meeting of the Endocrine Society. 2000, 163.
Wong A.O.L., Li W.S., Lee K.Y., Leung M.Y., Tse L.Y., Chow B.K.C., Lin H.R., Chang J.P., Pituitary adenylate cyclase activating polypeptide as a novel hypophysiotropic factor in fish., Biochemistry Cell Biology. 2000, 78: 329-343.
Yunker W.K., Lee K.Y., Wong A.O.L., Chang J.P., Norepinephrine Regulation of Growth Hormone Release from Goldfish Pituitary Cells. II. Intracellular Sites of Action., Journal of Neuroendocrinology. 2000, 12: 323-333.
Zhou H., Li W.S., Lee K.Y., Wong A.O.L., Autoregulation of Growth Hormone Synthesis in Grass Carp Pituitary Cells, In: H.J.Th. Goos, R.K. Rastogi, H. Vaudry, R. Pierantoni, Perspective in Comparative Endocrinology: Unity and Diversity. Monduzzi Editore S.p.A. - Medimond Inc., 2001, 827-833.


Researcher : Lee WWM
Project Title:Testicular proteases in sperm migration during spermatogenesis
Investigator(s):Prof. Lee W.W.M. , Dr. Cheng C.Y.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:August 1997
Abstract:
To study the functional characterization of testicular protease and look at its role in regulating the fomration and dissolution of junctional complexes.


Project Title:Mouse testin: genomic structure and construction of deletion vector for transgenic studies
Investigator(s):Prof. Lee W.W.M.
Source(s) of Funding:The Population Council, U.S.A.
Start Date:August 1997
Completion Date:June 2001
Abstract:
To carry out genomic structure and construction of deletion vector for transgenic studies.


Project Title:Functional characterization and transcriptional regulation of testin: a novel junctional complex protein in tissue reconstruction and spermatogenesis
Investigator(s):Prof. Lee W.W.M. , Dr. Cheng C.Y.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:October 1998
Abstract:
To examine the exon-intron organization of the mouse testin gene and its chromosomal location; to characterize the transcriptional start sites of the testin gene in the testis, ovary and lung; to identify the promoter sequences mediating tissue-specific expression of the testin gene; to characterize the trans-acting factors mediating tissue-specific expression of the testin gene; to construct a testin-deficient vector for use in gene knock-out transgenic study; to examine the mechanism by which testin an become tightly associated with the Sertoli cell membrane by characterizing the binding of testin onto the Sertoli cell membrane; identifying the testin binding protein on Sertoli cell membrane using cell surface labeling technique; and isolating tes tin receptor-like binding protein components for amino acid microsequencing.


Project Title:Analysis of the promoter sequence of rat and mouse testin
Investigator(s):Prof. Lee W.W.M.
Source(s) of Funding:The Population Council, U.S.A.
Start Date:July 1999
Abstract:
To analysis promoter sequence of rat and mouse testin.


Project Title:Gas6 expression and tyrosine kinase phosphorlation in testicular cell lines
Investigator(s):Prof. Lee W.W.M.
Source(s) of Funding:The Population Council, U.S.A.
Start Date:August 1999
Abstract:
To study Gas6 expression and tyrosine kinase phosphorlation in testicular cell lines.


Project Title:Formation and regulation of inter-Sertoli adhering, occluding and gap communication junctions in the testis
Investigator(s):Prof. Lee W.W.M.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:February 2000
Abstract:
To examine the physiological role of specialized junction molecules during junctional complexes formation, and their perturbation by external agents.


Project Title:Formation and regulation of specialized cell junctions in the testis during spermatogenesis
Investigator(s):Prof. Lee W.W.M. , Dr. Cheng C.Y.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 2000
Abstract:
To investigate the junctional complex components and to examine the specialized junction found in the testis. Specialized junction components identified will be used as markers to construct the cascade of events at different stages of the spermatogenic cycle leading to germ cell migration. Such a composite picture will be useful in identifying target(s) of intervention and contraception.


Project Title:Rabbit sex hormone binding globulin gene: postnatal expression, promoter sequence and transcriptional regulation
Investigator(s):Prof. Lee W.W.M.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:November 2000
Abstract:
To trace the ontogeny of shbg expression in the liver and testis by examining SHBG mRNA expression in neonatal rabbits using the northern blotting, and the plasma SHBG level using a steroid binding assay.


List of Research Outputs

Au C.L., Sy C.C., Lee W.W.M., Involvement of protein kinase A and C pathways in the regulation of vascular endothelial growth factor (VEGF) expression in Leydig Cells and Sertoli cells, 11th International Congress of Endocrinology, Sydney, Australia, October 29 - November 2. 2000.
Li J.C.H., Samy E.T., Grima J., Chung S.W., Mruk D., Lee W.W.M., Silvestrini B., Cheng C.Y., Rat Testicular Myotubularin, a Protein Tyrosine Phosphatase Expressed by Sertoli and Germ Cells, Is a Potential Marker for Studying Cell-Cell Interactions in the Rat Testis, Journal of Cellular Physiology. 2000, 185: 366-385.
Li J.C.H., Lee W.W.M., Mruk D., Cheng C.Y., Regulation of Sertoli Cell Myotubularin (rMTM) Expression by Germ Cells In Vitro, Journal of Andrology. 2001, 22: 266-277.
Lui W.Y., Lee W.W.M., Cheng C.Y., Transforming Growth Factor-b3 Perturbs the Inter-Sertoli Tight Junction Permeability Barrier in Vitro Possibly Mediated via Its Effects on Occludin, Zonula Occludens-1, and Claudin-11, Endocrinology. 2001, 142: 1865-1877.
Siu M.K.Y., Lee W.W.M., Cheng C.Y., Induction of Collagen Expression during Inter-Sertoli Tight Junction (TJ) Assembly in Vitro, 40th Annual Meeting of the American Society for Cell Biology, San Francisco, USA, Molecular Biology of the Cell, December 10 - 14. 2000, 11:564a.
Wong A.S.T., Lui W.Y., Hui I.T.C., Lee W.W.M., Rabbit sex hormone-binding globulin: expression in the liver and testis during postnatal development and structural characterization by truncated proteins, International Journal of Andrology. 2001, 24: 165-174.


Researcher : Leung FCC
Project Title:Use of transgenic chicken as bioreactor
Investigator(s):Dr. Leung F.C.C.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:October 1996
Completion Date:September 2000
Abstract:
To study the use of transgenic chicken as bioreactor.


Project Title:Genetic marker for the mouse wobbler mutation
Investigator(s):Dr. Leung F.C.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Abstract:
To develop genetic marker using repetitive sequences as probes for the early detection of mouse wobbler mutation.


Project Title:Use of multimedia for demonstrating laboratory practical
Investigator(s):Dr. Leung F.C.C.
Source(s) of Funding:Run Run Shaw Research and Teaching Endowment Fund
Start Date:March 1999
Abstract:
To use multimedia program for lecturers to use in conjunction with the molecular biology laboratory practical. The program is mean to provide a new visual and graphical aids to help students in lab practical, and promote better learning environment. This new approach, if proven to be successful, can be extended to other laboratory courses within the Department, Faculty, or Institution levels.


Project Title:Isolation and characterization of the porcine reproductive and respiratory syndrome virus
Investigator(s):Dr. Leung F.C.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To develop molecular genetic assay for typing the Porcine Reproductive and Respiratory Syndrome Virus (PRRS).


Project Title:Molecular characterization of the chicken growth hormone receptor gene
Investigator(s):Dr. Leung F.C.C.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:February 2000
Abstract:
To identify, isolate, clone, and sequence the genomic chicken GHR gene, and we will provide the badly needed non-mammalian GHR genomic gene for the comparative study of the evolution of the growth hormone receptor gene.


List of Research Outputs

Ip C.Y., Zhang X.Q., Leung F.C.C., Genomic Growth Hormone Gene Polymorphisms in Native Chinese Chickens, Proceedings of the Society of Experimental Biology and Medicine. 2001, 226: 458-462.
Ip S., Chan M.N., Leung F.C.C., Characterization of the chicken genomic growth hormone gene (Wai Chow strain)., Poultry Science. 2000, 79(supp. I): 10.
Leung F.C.C., Zeng F., Characterization of A Novel Repetitive DNA Fragment from the Panax gingeng genome, Proceedings of the International Ginseng Workshop, Utilization of biotechnologal, genetic and cultural approaches for North American and Asian ginseng improvement. Editor, Zamir K. Punja. 2001, 119-124.
Li J., Wong X., Leung F.C.C., Molecular cloning and characterization of a novel repetitive DNA family from chicken., Poultry Science. 2000, 79(supp. I): 10.
Wang Y., Leung F.C.C., Cross F., Welt M., Tan S.J., Yang S.Q., Cloning of an Alu-like Sequence from Rat Low-Cot DNA Library and Its Application in the Analysis of Genomic DNA Mutation, Chinese Journal of Applied Environmental Biology. 2000, 6: 48-51.
Wu H., Liu F., Leung F.C.C., A recombinant infectious laryngotracheitis virus (ILTV) expressing the spike gene (S1) of the infectious bronchitis virus (IBV)., Poultry Science. 2000, 79(Supp. I): 48.
Wu H., Liu F., Xing C., Leung F.C.C., S1 gene diversity of chicken infectious bronchitis virus (IBV) in China., Poultry Science. 2000, 79(supp. I): 48.
Zhang X., Leung F.C.C., Chan D.K.O., Yang G., Wu C., Genetic diversity of Chinese native chickens, China-EU Workshop on Animal Biotechnology, Shenzhen 10-13 October. 2000.


Researcher : Leung PC
Project Title:To study the action of pituitary adenylate cyclase activating polypeptide
Investigator(s):Dr. Leung P.C.
Source(s) of Funding:Croucher Foundation
Start Date:September 1996
Abstract:
To find out (i) if the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP) will interact with calmodulin in the cell as part of its mode of action in the cell, (ii) what kind of calmodulin-mediated processes in the cell is affected if PACAP does interact with calmodulin in the cell, and (iii) the protein-phosphorylation cascade in the cell induced by PACAP.


Project Title:Characterization of ACC N-malonyltransferase from mung bean
Investigator(s):Dr. Leung P.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1997
Abstract:
To study the enzyme in detail, to find out how the mechanism of the enzymatic reaction, and to see if the enzyme can malonylate D-amino acids.


Project Title:Characterization of a new form of the fyn oncogene
Investigator(s):Dr. Leung P.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Abstract:
To characterize this new variant form of the fyn oncogene, so as to assess the significance of this new fyn gene in the cell.


Project Title:To identify the factor that inhibits reverse transcription in mung bean seedling hypocotyl
Investigator(s):Dr. Leung P.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
There is some thing unusual about the mung bean seedling hypocotyl. We think that there is a factor in the hypocotyl that inhibits reverse transcription. This project is to find out if this factor exist or not, and, if it exists, to isolate it.


Project Title:To study phytotoxin resistance in transformed plant cells
Investigator(s):Dr. Leung P.C.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:November 2000
Abstract:
To establish a system for the transfer of foreign genes into the plant cells.


List of Research Outputs

Au T.K., Chick W.S.H., Leung P.C., The biology of ophiobolins, Life Sciences. Elsevier Science Inc., 2000, 67: 733-742.
Lee K.Y., Chan P.H., Au T.K., Leung P.C., Wong A.O.L., Goldfish calmodulin: Molecular cloning, tissue distribution, and regulation of its mRNA expression in goldfish pituitary cells., 4th International Symposium on Fish Endocrinology. 2000, 80.


Researcher : Li ETS
Project Title:The impact of diet restriction on body weight and energy metabolism of young overweight males
Investigator(s):Dr. Li E.T.S.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1995
Completion Date:December 2000
Abstract:
To examine energy metabolism and energy intake in young overweight/obese men and to correlate these parameters with body weight and weight change at different stages of a diet restriction programme.


Project Title:Delineating the relationship between body fat and body mass index in Chinese adults
Investigator(s):Dr. Li E.T.S.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1996
Completion Date:December 2000
Abstract:
To provide reference values of anthropometry and resting metabolic rate for Hong Kong adults and to develop population specific prediction equations for the estimation of body composition.


Project Title:Serotonin metabolism in diet-induced obese rats
Investigator(s):Dr. Li E.T.S.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1997
Completion Date:December 2000
Abstract:
To characterize the serotonergic deficit in the lateral hypothalamus of diet-induced obese rats.


Project Title:Application of embryo transplant technology to the study of diet-induced obesity in rats
Investigator(s):Dr. Li E.T.S. , Prof. Chan S.T.H.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Completion Date:December 2000
Abstract:
To generate DIO and DR Sprague-Dawley rats through selective breeding; to transfer embryos of DIO and DR rats to the uterus of pseudopregnant DR and DIO female rats, respectively; to determine the impact of high fat diet on the growth rate of the progeny; to determine body fat content, plasma insulin and glucose tolerance of the offspring.


Project Title:Body mass index, adiposity, serum leptin and insulin sensitivity in Chinese and Caucasians: a comparative study
Investigator(s):Dr. Li E.T.S. , Prof. Anderson G.H., Prof. Jenkins D., Prof. Kung A.W.C., Prof. Leiter L., Dr. Tan K.C.B.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:December 2000
Abstract:
To compare healthy Chinese and Caucasions to study the association between, first, body mass index (BMI) and body fat and insulin sensitivity, and second BMI and glucose disposal rate. Results of this study will provide information required for assessing the validity of the international criteria for obesity and may lead to establishing specific overweight and obesity definitions for Chinese population.


List of Research Outputs

He M., Tan K.C.B., Li E.T.S., Kung A.W.C., Body fat determination by dual energy X-ray absorptiometry and its relation to body mass index and waist circumference in Hong Kong Chinese., International Journal of Obesity. 2001, 25: 748-752.
Li E.T.S., Tang E.K.Y., Wong C.Y.M., Lui S.S.H., Chan V.Y.N., Dai D.L.K., Predicting stature from knee height in Chinese elderly subjects, Asia Pacific Journal of Clinical Nutrition. 2000, 9: 252-255.


Researcher : Li J
List of Research Outputs

Li J., Wong X., Leung F.C.C., Molecular cloning and characterization of a novel repetitive DNA family from chicken., Poultry Science. 2000, 79(supp. I): 10.


Researcher : Lim BL
Project Title:Molecular characterization of a new foot-and-mouth disease virus strain isolated in Hong Kong
Investigator(s):Dr. Lim B.L.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Abstract:
To sequence the full length genome (approximate 8 kb) of the local FMDV strain.


Project Title:Investigation of the pathogenicity of the infectious bursal disease virus by reverse genetics technology
Investigator(s):Dr. Lim B.L.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:November 1998
Completion Date:June 2001
Abstract:
Infectious bursal disease (IBD), caused by infectious bursal disease virus (IBDV), is a highly contagious disease of young chickens, which results in significant losses to the poultry industry. After infection, IBDV multiplies rapidly in the developing B lymphocytes of the bursa of Fabricius, leading to immunosuppression and increased susceptibility to other diseases. According to antigenic variation and virullence, serotype I strains can be divided into several groups: classical virulent strains, a ttenuated strains, antigenic variant strains and 'very virulent' strains. After characterizing the gene of the neutralizing antigen of eight Chinese and local IBDV field strains, certain amino acid residues affecting the virulence of the virus have bee n suggested. In this project, the whole genome of the IBDV virus will be duplicated by reverse genetics technology so that recombinant viruses with mutated amino acids can be generated. It is expected that this investigation can figure out the critica l amino acids residues affecting the virulence of the virus and generate a live vaccine strain which can confer adequate protection to the broilers from the attack of the 'very virulent' IBDV field strains.


Project Title:Acute infectious bursal disease in poultry: epidemiology of the disease, basis of virulence and improvement of vaccination
Investigator(s):Dr. Lim B.L. , Dr. Eterradossi N., Prof. Muller H., Dr. van den Berg T.
Source(s) of Funding:European Commission International Cooperation
Start Date:January 1999
Abstract:
To study: 1) development and harmonisation of the diagnostic tools; 2) molecular epidemiology of vvIBDV; 3) constitution of an IBDV genomic cDNA bank and generation of relevant full length cDNA's that could be used in the reverse genetics system to generate genetically engineered recombinant infectious viral clones; 4) harmonisation of nomenclature of IBDV strains based on molecular criteria; 5) pathogenesis of the disease: search for cell receptors and virulence markers - molecular basis of attenuation - cell-mediated immune response; 6) improvement of the vaccination protocols: reverse genetics system - DNA vaccination.


Project Title:Generation of VP5-deletion mutants from recombinant IBDV viruses by reverse genetics
Investigator(s):Dr. Lim B.L.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To generate more recombinant mutants by site-directed mutagenesis and reverse genetics technolgoy, which can be used to evaluate the effects of certain amino acids on the pathogenicity of the virus.


Project Title:Identification and molecular cloning of infectious bursal disease virus (IBDV) receptors on chicken cells
Investigator(s):Dr. Lim B.L.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:February 2000
Abstract:
To clone and identify the receptors of infectious bursal disease virus (IBDV) by both molecular and biochemical approaches.


Project Title:Identification and molecular cloning of infectious bursal disease virus (IBDV) receptors on chicken cells
Investigator(s):Dr. Lim B.L.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:February 2000
Abstract:
To clone and identify the receptors of infectious bursal disease virus (IBDV) by both molecular and biochemical approaches.


List of Research Outputs

Ghebrehiwet B., Lim B.L., Kumar R., Feng X., Peerschke E.I.B., gC1qR/p33: A member of a new class of multifunctional and multicompartmental cellular proteins (MMCPs) is involved in inflammation and infection., Immunological Review. 2001, 180: 65-77.
Mo C.W., Cao Y.C., Lim B.L., The In Vivo and In Vitro Effects of Chicken Interferon a on Infectious Bursal Disease Virus and Newcastle Disease Virus Infection, Avian Diseases. 2001, 45: 389-399.
Tye A.J., Ghebrehiwet B., Guo N., Sastry K.N., Chow B.K.C., Peerschke E.I.B., Lim B.L., The Human gC1qR/p32 Gene, C1qBP, Journal of Biological Chemistry. 2001, 276: 17069-17075.
Xue C., Lim B.L., In Situ Localization of Infectious Bursal Disease Virus-Binding Cells by a Biotin-Streptavidin System, Avian Diseases. 2001, 45: 504-511.


Researcher : Lui WY
List of Research Outputs

Wong A.S.T., Lui W.Y., Hui I.T.C., Lee W.W.M., Rabbit sex hormone-binding globulin: expression in the liver and testis during postnatal development and structural characterization by truncated proteins, International Journal of Andrology. 2001, 24: 165-174.


Researcher : Lun HM
List of Research Outputs

Lun H.M., Lai Y.Y., Mak C., Ko R.C.C., Identification and molecular cloning of metalloprotease in the excretory/secretory products of the infective-stage larvae of Trichinella spiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 40.


Researcher : Mak C
Project Title:Identification of heat shock regulated transcripts and its role in adaptive responses of an intracellular parasite, Trichinella
Investigator(s):Dr. Mak C.H. , Prof. Ko R.C.C.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Completion Date:June 2001
Abstract:
To use differential display and suppressive subtractive hybridization methods to isolate genes specifically expressed in heat shocked T. spiralis and T. pseudospiralis; to identify potential targets for the diagnosis and treatment of Trichinosis.


Project Title:The suppression of host inflammatory response by an intracellular parasitic nematode
Investigator(s):Dr. Mak C.H. , Prof. Ko R.C.C.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:February 2000
Completion Date:January 2001
Abstract:
To determine the role of ES products on host immune response, in vitro effects of the products on the cellular cytokine secretion profile is investigated.


List of Research Outputs

Lun H.M., Lai Y.Y., Mak C., Ko R.C.C., Identification and molecular cloning of metalloprotease in the excretory/secretory products of the infective-stage larvae of Trichinella spiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 40.
Mak C., Ko R.C.C., Characterization of endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 27.
Mak C., Chung Y.Y.Y., Ko R.C.C., Single-stranded endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., Parasitology. 2000, 120: 527-533.
Wong C.S., Mak C., Ko R.C.C., Single-stranded endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 41.
Wu W.K., Sun C.K., Mak C., Ko R.C.C., Characterization of genes differentially expressed in Trichinella spiralis by heat shock treatment using suppression subtractive hybridization., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 38.


Researcher : Ng SM
List of Research Outputs

Ng S.M., Yu K.L., Yung W.H., Chow B.K.C., Real-Time Analysis of the Activities of GnRH and GnRH Analogs in aT3-1 Cells by the Cytosensor Microphysiometer, Journal of Cellular Biochemistry. 2001, 80: 304-312.


Researcher : Pang TKR
List of Research Outputs

Chow B.K.C., Tse L.Y., Pang T.K.R., Wong A.O.L., Identification of a novel PHI receptor in goldfish: Implication on the evoluation of VIP/PACAP receptors in vertebrates., 20th Conference of European Comparative Endocrinologists. 2000, #8S: 88.


Researcher : Silva Gunawardene YIN
List of Research Outputs

Silva Gunawardene Y.I.N., Chow B.K.C., He J.G., Chan S.M., The shrimp FAMeT cDNA is encoded for a putative enzyme involved in the methylfarnesoate (MF) biosynthetic pathway and is temporally expressed in the eyestalk of different sexes, Insect Biochemistry and Molecular Biology. 2001, 31: 1115-1124.


Researcher : Sun M
Project Title:Molecular population genetics of colonizing success of yellow starthistle (centaurea solstitialis L.)
Investigator(s):Dr. Sun M.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1996
Completion Date:August 2000
Abstract:
Yellow starthistle is an aggressive colonizing plant originating in the Middle East which has become a serious weed in much of the Pacific rim. The process of colonization is an important theme in evolutionary biology, yet has been inadequately studied, especially with the use of modern molecular techniques. I will investigate the population genetic structure of yellow starthistle populations from the center of origin and from colonized sites, using molecular and biochemical markers. This will help elucidate the genetic consequences of colonization in this model species, and give indicators to early control strategies to follow as it emerges as a weed in further locations in south east Asia.


Project Title:Population and conservation genetics of wild orchids in Hong Kong
Investigator(s):Dr. Sun M.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1997
Abstract:
To evaluate the current species status to identify management units and determine taxa for priority conservation; to explore patterns of genetic variation within and among populations of selected priority species; to investigate factors affecting this variation and structure, such as habitat fragmentation, population size and mating system; to make recommendation for conservation of species and genetic diversity of wild orchids based on the results obtained.


Project Title:Assessing genetic diversity and relationships in amaranthus germplasm using DNA markers
Investigator(s):Dr. Sun M.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:December 1998
Abstract:
To assess genetic diversity and relatedness among accessions in the germplasm at both intra- and interspecific level using a set of Amaranthus-specific low-Cot DNA markers; to understand the evolutionary origin of the three most important grain species: A. caudatus, A. cruentus, and A. hypochondriacus, with emphasis on their relationship to each other and to their three putative wild progenitors: A. hybridus, A. powellii, and A. quitensis, using cpDNA and mtDNA polymorphisms in addition to the use of other DNA markers; to evaluate the applicability and effectiveness of these DNA markers, in comparison to allozymes and RAPDs, in population genetic assays and assessing phylogenetic relationships between Amaranthus accessions; to make recommendations for conservation management and the utilization of Amaranthus germplasm based on the results obtained from these studies.


Project Title:Genetic variation of Chinee Larix species
Investigator(s):Dr. Sun M. , Dr. Lascoux D.M., Dr. Zhang H.Q.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To investigate population genetic variation and structure of Chinese Larix species; to study geographical distribution, genetic distances, and evolutionary relationships among these species, and the existence and extent of hybrid zones will be checked using both nucelar and cytoplasmic markers; to compare Chinse Larix species with previously sudies Siberian larch species for reconstruction of the recolonization history of Siberian larch species using cytoplasmic markers, especially mitochondrial DNA that is maternally inherited and therefore only dispersed through seeds; to explore the applicability of population genetic information in the conservation and management of Larix species in China and world-wide.


Project Title:Population genetics, phylogenetic relationships, and conservation of mangroves
Investigator(s):Dr. Sun M. , Dr. Duke N.C.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 2000
Abstract:
To provide baseline information pertinent to genetic conservation and management of mangroves. The project focuses on the genetic diversity of important mangrove species and factors affecting this diversity, adn to establish the identity of Rhizophora taxa for conservation management.


List of Research Outputs

Cai Y., Sun M., Schliemann W., Corke H., Chemical stability and colorant properties of betaxanthin pigments from Celosia argentea, Journal of Agricultural and Food Chemistry. 2001, 49: 4429-4435.
Cai Y., Sun M., Corke H., Identification and distribution of simple and acylated betacyanins in the Amaranthaceae, Journal of Agricultural and Food Chemistry. 2001, 49: 1971-1978.
Lascoux M., Sun M., The neutral theory of molecular evolution: 30 years later, Zu, Y., Sun, M., and Kang, L. The Molecular Mechanisms of Ecological Adaptation and Evolution. CHEP-Springer, 2000, 57-86.
Sun M., Allozyme analysis of natural populations: some case studies in plant evolution, International symposium and workshop on plant evolution using allozymes as genetic markers, 15-16 December, Chinju, Korea. 2000.
Sun M., Lascoux M., The development of molecular ecology: an amalgam of new molecular genetic tools with established fields, Zu, Y., Sun, M., and Kang, L. The Molecular Mechanisms of Ecological Adaptation and Evolution. CHEP-Springer, 2000, 34-56.
Tan Y.F., Xing Y.Z., Zhang Q., Sun M., Corke H., Quantitative genetic basis of gelatinization temperature of rice, Cereal Chemistry. 2001, 78: 666-674.
Zu Y., Sun M., Kang L., The Molecular Mechanisms of Ecological Adaption and Evolution, CHEP-Springer, 2000, 238 pp.


Researcher : Tan-Un KC
Project Title:The study of mitochondrial DNA mutations in Chinese patients
Investigator(s):Dr. Tan-Un K.C. , Dr. Chan J.H.M., Dr. Cheng L.Y.L., Dr. Shen J.H.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To identify the mtDNA mutations in Chinese families with mitochondrial disease; to establish, using primers designed to study mtDNA mutations, the frequencies of these mtDNA mutations in the Chinese population.


List of Research Outputs

Chan Y.M., Tsui T.K., Lai C.K.W., Ip M., Tan-Un K.C., C-590 polymorphism of IL-4 promoter and asthma among Chinese, European Respiratory Society Annual Congress, Florence, Italy, Sept.. 2000, 2303.
Flint J., Tufarelli C., Peden J., Clark K., Daniels R.J., Hardison R., Miller W., Philipsen S., Tan-Un K.C., Mcmorrow T., Frampton J., Alter B.P., Frischauf A.M., Higgs D.R., Comparative genome analysis delimits a chromosomal domain and identifies key regulatory elements in the a globin cluster, Human Molecular Genetics. 2001, 10: 371-382.
Tan-Un K.C., Lam H.K., Lam W.K., Sham A., Chan Y.M., Glutathione S-transferase polymorphisms M1 and T1 and susceptibility to lung cancer in the Chinese population, European Respiratory Society Annual Congress, Florence, Italy, Sept.. 2000, 285.


Researcher : Tye AJ
List of Research Outputs

Tye A.J., Ghebrehiwet B., Guo N., Sastry K.N., Chow B.K.C., Peerschke E.I.B., Lim B.L., The Human gC1qR/p32 Gene, C1qBP, Journal of Biological Chemistry. 2001, 276: 17069-17075.


Researcher : Wan JMF
Project Title:The anti-inflammatory properties of Yun Zhi
Investigator(s):Dr. Wan J.M.F.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Completion Date:April 2001
Abstract:
To investigate whether Yun Zhi exhibits some antinflammatory properties such as suppression of cytokines, reduce shock, maintain good appetite and weight gain.


Project Title:The anticancer mechanisms of Denshen
Investigator(s):Dr. Wan J.M.F.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To study its effects on both cellular proliferation and cell death by flow cytometry technology.


List of Research Outputs

Chiu L.C.M., Wan J.M.F., Ooi V.E.C., Induction of apoptosis by dietary polyunsaturated fatty acids in human leukemic cells is not associated with DNA fragmentation, International Journal of Oncology. 2000, 17: 789-796.
Lee J.C.Y., Wan J.M.F., Vitamin E Supplementation Improves Cell-Mediated Immunity and Oxidative Stress of Asian Men and Women, Journal of Nutrition. 2000, 2932-2937.


Researcher : Wang F
List of Research Outputs

Huang M., Wang F., Xu R.J., Biological characteristics fo leptin and its role in metabolism and reproduction, Animal Husbandry & Veterinary Medicine. 2000, 32: 40-43.
Xu R.J., Wang F., Biological significance and practical application of milk-borne bioactive peptides, Progress in Veterinary Medicine. 2000, 21: 8-11.
Xu R.J., Wang F., Zhang S.H., Postnatal adaptation of the gastrointestinal tract in neonatal animals: possible role of milk-borne growth factors, Livestock Production Science. 2000, 66: 95-107.
Xu R.J., Wang F., The role of milk-borne growth factors in regulating postnatal adaptation of the gastrointestinal tract in neonatal pigs, Animal Science and Veterinary Medicine. 2000, 17: 10-14.


Researcher : Wong AOL
Project Title:Pituitary adenylate cyclase activating polypeptide as a novel growth hormone releasing factor in Chinese grass carp, Ctenopharyngodon idellus
Investigator(s):Dr. Wong A.O.L.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:January 1999
Abstract:
To establish the functional role of PACAP as a novel regulator of GH secretion and body growth in the grass carp.


Project Title:Role of cAMP in pituitary adenylate cyclase-activating polypeptide regulation of growth hormone gene expression in Chinese grass carp
Investigator(s):Dr. Wong A.O.L.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To examine the effect of PACAP on steady state GH mRNA levels in grass carp pituitary cells; to test if activation of the cAMP pathway can mimic the effect of PACAP on GH mRNA levels; to test if inhibition of the cAMP pathway can abolish the effect of PACAP on GH mRNA levels; to elucidate the role of mRNA transcription in PACAP- and cAMP-regulated GH gene expression; to elucidate the role of mRNA stability in PACAP- and cAMP-regulated GH gene expression.


Project Title:Biphasic growth hormone release induced by protein kinase C activation in grass carp pituitary cells
Investigator(s):Dr. Wong A.O.L.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:February 2001
Abstract:
To elucidate the neuroendocrine and signal transduction mechnaisms regulating growth hormone (GH) synthesis and secretion in fish.


Project Title:Grouper growth hormone: production of recombinant protein and its applications in green grouper farming
Investigator(s):Dr. Wong A.O.L.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 2001
Abstract:
To conduct molecular cloning of growth hormone cDNA in groupers; to express recombinant growth hormone using a yeast expression system; to test its potential applications in grouper farming both in adult fish as well as in juvenile fish.


List of Research Outputs

Chang J.P., Johnson J.D., Van Goor F., Wong C.J.H., Yunker W.K., Uretsky A.D., Taylor D., Jobin R.M., Wong A.O.L., Goldberg J.I., Signal transduction mechanisms mediating secretion in goldfish gonadotropes and somatotropes., Biochemistry Cell Biology. 2000, 78: 139-153.
Chow B.K.C., Tse L.Y., Pang T.K.R., Wong A.O.L., Identification of a novel PHI receptor in goldfish: Implication on the evoluation of VIP/PACAP receptors in vertebrates., 20th Conference of European Comparative Endocrinologists. 2000, #8S: 88.
Koshimizu T.A., Van Goor F., Tomic M., Wong A.O.L., Tanoue A., Tsujimoto G., Stojilkovic S.S., Characterization of Calcium Signaling by Purinergic Receptor-Channels Expressed in Excitable Cells, Molecular Pharmacology. 2000, 58: 936-945.
Koshimizu T.A., Tomic M., Wong A.O.L., Zivadinovic D., Stojilkovic S.S., Characterization of Purinergic Receptors and Receptor-Channels Expressed in Anterior Pituitary Cells, Endocrinology. 2000, 141: 4091-4099.
Lee K.Y., Chan P.H., Au T.K., Leung P.C., Wong A.O.L., Goldfish calmodulin: Molecular cloning, tissue distribution, and regulation of its mRNA expression in goldfish pituitary cells., 4th International Symposium on Fish Endocrinology. 2000, 80.
Lee K.Y., Chan V.C.C., Chang J.P., Yunker W.K., Wong A.O.L., Norepinephrine Regulation of Growth Hormone Release from Goldfish Pituitary Cells. I. Involvement of a2 Adrenoreceptor and interactions with Dopamine and Salmon Gonadotropin-Releasing Hormone, Journal of Neuroendocrinology. 2000, 12: 311-322.
Li W.S., Chu M.M.S., Lin H.R., Wong A.O.L., Interaction of pituitary adenylate cyclase-activating polypeptide with gonadotropin-releasing hormone, dopamine, and somatostatin in regulating growth hormone gene expression in grass carp pituitary cells., 4th International Symposium on Fish Endocrinology.. 2000, 82.
Li W.S., Leung R.C.Y., Lee K.Y., Lin H.R., Wong A.O.L., Pituitary adenylate cyclase-activating polypeptide stimulates growth hormone release and synthesis in grass carp pituitary cells., 82nd Annual Meeting of the Endocrine Society. 2000, 163.
Van Goor F., Zivabinovic D., Wong A.O.L., Stojilkovic S.S., Calcium-activated, voltage-dependent K+ (BK) channels account for differences in spiking pattern between spontaneously active rat somatotrophs, lactotrophs and gonadotrophs., 2000 Annual Meeting of the Society for Neuroscience 2000. 1841.
Wong A.O.L., Li W.S., Lee K.Y., Leung M.Y., Tse L.Y., Chow B.K.C., Lin H.R., Chang J.P., Pituitary adenylate cyclase activating polypeptide as a novel hypophysiotropic factor in fish., Biochemistry Cell Biology. 2000, 78: 329-343.
Yunker W.K., Lee K.Y., Wong A.O.L., Chang J.P., Norepinephrine Regulation of Growth Hormone Release from Goldfish Pituitary Cells. II. Intracellular Sites of Action., Journal of Neuroendocrinology. 2000, 12: 323-333.
Zhou H., Li W.S., Lee K.Y., Wong A.O.L., Autoregulation of Growth Hormone Synthesis in Grass Carp Pituitary Cells, In: H.J.Th. Goos, R.K. Rastogi, H. Vaudry, R. Pierantoni, Perspective in Comparative Endocrinology: Unity and Diversity. Monduzzi Editore S.p.A. - Medimond Inc., 2001, 827-833.


Researcher : Wong CS
List of Research Outputs

Wong C.S., Mak C., Ko R.C.C., Single-stranded endonuclease activity in the excretory/secretory products of Trichinella spiralis and Trichinella pseudospiralis., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 41.


Researcher : Wu H
List of Research Outputs

Wu H., Liu F., Leung F.C.C., A recombinant infectious laryngotracheitis virus (ILTV) expressing the spike gene (S1) of the infectious bronchitis virus (IBV)., Poultry Science. 2000, 79(Supp. I): 48.
Wu H., Liu F., Xing C., Leung F.C.C., S1 gene diversity of chicken infectious bronchitis virus (IBV) in China., Poultry Science. 2000, 79(supp. I): 48.


Researcher : Wu WK
List of Research Outputs

Wu W.K., Sun C.K., Mak C., Ko R.C.C., Characterization of genes differentially expressed in Trichinella spiralis by heat shock treatment using suppression subtractive hybridization., 10th International Conference on Trichinellosis, Fontainbleau, France. 2000, 38.


Researcher : Xu RJ
Project Title:Postnatal adaptation of the gastrointestinal tract in newborns: a possible role of milk-borne transforming growth factor-beta
Investigator(s):Dr. Xu R.J.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Abstract:
To examine the role of milk-borne TGF-beta in the postnatal adaptation of the gastrointestinal tract.


Project Title:Effect of milk-borne growth factors on gastrointestinal development in newborns
Investigator(s):Dr. Xu R.J.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:January 1999
Abstract:
To test the hypothesis that milk-borne growth factors stimulate gastrointestinal maturation in newborns, and to evaluate whether milk-borne growth factors can be used to enhance gastrointestinal maturation in newborns of premature birth and/or of prenatal growth retardation.


Project Title:Bioactive peptides from enzymatic hydrolysate of milk protein: biological and health implications
Investigator(s):Dr. Xu R.J.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To develop a processing technique to generate bioactive peptides from milk proteins and to produce value added dairy products.


List of Research Outputs

Chan S.K., Xu R.J., Determination of transforming growth factor-b1 and TGF-b2 in porcine colostrum and milk, Proceedings of the Nutrition Society of Australia. 2000, 24: 253.
Ding H.B., Xu R.J., Chan D.K.O., Meat quality comparison between broilers and Chinese local chickens, Animal Husbandry and Veterinary Medicine. 2000, 32: 16-18.
Ding H.B., Xu R.J., Near-infrared spectroscopic technique for detection of beef hamburger adulteration, Journal of Agricultural and Food Chemistry. 2000, 48: 2193-2198.
Huang M., Wang F., Xu R.J., Biological characteristics fo leptin and its role in metabolism and reproduction, Animal Husbandry & Veterinary Medicine. 2000, 32: 40-43.
Li Z., Liu X.P., Zhang Y.Q., Xu R.J., Zhang S.H., Identification and sequencing of preprothyrotropin-releasing hormone and its receptor in rat testis, Journal of Medical College of PLA. 2000, 15: 146-149.
Xu R.J., Wang F., Biological significance and practical application of milk-borne bioactive peptides, Progress in Veterinary Medicine. 2000, 21: 8-11.
Xu R.J., Wang F., Zhang S.H., Postnatal adaptation of the gastrointestinal tract in neonatal animals: possible role of milk-borne growth factors, Livestock Production Science. 2000, 66: 95-107.
Xu R.J., Wang F., The role of milk-borne growth factors in regulating postnatal adaptation of the gastrointestinal tract in neonatal pigs, Animal Science and Veterinary Medicine. 2000, 17: 10-14.
Yan T.K.E., Cheng T.H., Xu R.J., Isolation of angiotensin converting enzyme from pig lung, Proceedings of the Nutrition Society of Australia. 2000, 24: 151.


Researcher : Xue C
List of Research Outputs

Xue C., Lim B.L., In Situ Localization of Infectious Bursal Disease Virus-Binding Cells by a Biotin-Streptavidin System, Avian Diseases. 2001, 45: 504-511.


Researcher : Yan TKE
List of Research Outputs

Yan T.K.E., Cheng T.H., Xu R.J., Isolation of angiotensin converting enzyme from pig lung, Proceedings of the Nutrition Society of Australia. 2000, 24: 151.


Researcher : Yeung HY
List of Research Outputs

Wong C.K.C., Yeung H.Y., Mak N.K., Dimattia G.E., Chan D.K.O., Wagner G.F., Effects of Dibutyryl cAMP on Stanniocalcin and Stanniocalcin-Related Protein mRNA Expression in Neuroblastoma Cells, 82nd Annual Meeting of Endocrine Society, Toronto, Canada. 2000.


Researcher : Yu KL
Project Title:Cloning and expression of the gene for the goldfish dopamine D2 receptor
Investigator(s):Dr. Yu K.L. , Dr. Dong K.W.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:August 1994
Abstract:
To obtain a better understanding of the mechanism underlying the regulation of dopamine D2 receptor biosynthesis in the teleost pituitary using molecular approach; there are two basic parts to this proposal; the first is to isolate the structure of the D2 receptor genes involved in the regulation of pituitary gonadotropin (GtH) secretion in goldfish; such information is crucial to the development of oligonucleotide probes for the study of the regulation of D2 receptor gene expression; the application of molecular techniques to study fish neurotransmitter receptor that is involved in reproduction also has enormous potential to be of considerable benefit to aquaculture; the elucidation of the transcriptional regulatory mechanism for D2 receptor gene expression will ultimately allow possible engineering of the gene and/or its regulatory element to manipulate sexual maturity in the goldfish and in many other potentially important aquacultural species.


Project Title:Molecular cloning and physiological studies of the gonadotropin-releasing hormone (GnRH) receptor genes in goldfish
Investigator(s):Dr. Yu K.L. , Prof. Chang J.P., Prof. Peter R.E.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:August 1995
Abstract:
To obtain a better understanding of the mechanism underlying the regulation of GnRH receptor in the fish pituitary using molecular approach.


Project Title:Neural regulation of gonadotropin and growth hormone gene expression in goldfish pituitary
Investigator(s):Dr. Yu K.L.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1996
Abstract:
To acquire basic information on neural regulation of pituitary gonadotropin (GtH) and growth hormone (GH) gene expression in goldfish; to elucidate the structure of beta-endorphin which is involved in the regulation of both pituitary GtH and GH functions.


Project Title:Molecular cloning and physiological studies of the gonadotropin-releasing hormone genes in fish
Investigator(s):Dr. Yu K.L.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1996
Abstract:
To obtain a better understanding of the mechanism underlying the regulation of biosynthesis of multiple GnRH peptides in the teleost brain using molecular approach.


Project Title:A spawning kit for induced reproduction in marine fishes using synthetic GnRH and receptor technology
Investigator(s):Dr. Yu K.L. , Prof. Chan D.K.O.
Source(s) of Funding:Industrial Support Fund, Industry Department of Hong Kong Government
Start Date:July 1997
Abstract:
To design and apply GnRH peptide analogs acting on specific pituitary GnRH receptor subtypes to accelerate sexual maturity and spawning in marine reef fishes.; to develop and produce a third-generation "spawning kit" for the aquaculture industry.


Project Title:Transcriptional regulation of tissue-specific gonadotropin releasing hormone gene expression
Investigator(s):Dr. Yu K.L.
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1997
Abstract:
To identify and characterize the cellular and molecular mechanisms which regulate tissue-specific expression of salmon gonadotropin-releasing hormone (sGnRH) and chicken GnRH-II (cGnRH-II) genes in goldfish.


Project Title:Nitric oxide signaling in the neuroendocrine regulation of pituitary functions in goldfish
Investigator(s):Dr. Yu K.L. , Dr. Xie Y.W.
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1998
Abstract:
To investigate the novel roles of nitirc oxide (NO) and its signaling cascade in the neuroendocrine regulation of pituitary hormone secretion in goldfish. Specifically, the roles of NO in the regulation of in vitro release of neurohormones (gonadotropin-releasing hormone or GnRH and growth hormone releasing hormone or GRF) and pituitary hormones including gonadotropin (GtH) and growth hormone (GH) will be studied using both static incubation and perifusion sytems. To determine tissue distribution of neuronal NOS (nNOS) proteins and mRNA in goldfish brain regions and its possible co-localization with the GnRH and GRF neurons.


Project Title:Transcriptional mechanisms regulating tissue-specific expression of two gonadotropin releasing hormone receptor genes in goldfish
Investigator(s):Dr. Yu K.L.
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:February 2000
Abstract:
To identify and characterize the cellular and molecular mechanisms which regulate tissue-specfic expression of GnRH-R genes in goldfish.


List of Research Outputs

Lee K.Y., Ko K.W., Chan D.K.O., Dong K.W., Yu K.L., Cloning and characterization of A gonadotropin-releasing hormone receptor in the seabream (Mylio macrocephalus), 82nd Annual Meeting of Endocrine Society, Toronto, Canada. 2000.
Ng S.M., Yu K.L., Yung W.H., Chow B.K.C., Real-Time Analysis of the Activities of GnRH and GnRH Analogs in aT3-1 Cells by the Cytosensor Microphysiometer, Journal of Cellular Biochemistry. 2001, 80: 304-312.


Researcher : Zeng F
List of Research Outputs

Leung F.C.C., Zeng F., Characterization of A Novel Repetitive DNA Fragment from the Panax gingeng genome, Proceedings of the International Ginseng Workshop, Utilization of biotechnologal, genetic and cultural approaches for North American and Asian ginseng improvement. Editor, Zamir K. Punja. 2001, 119-124.


Researcher : Zhou H
List of Research Outputs

Zhou H., Li W.S., Lee K.Y., Wong A.O.L., Autoregulation of Growth Hormone Synthesis in Grass Carp Pituitary Cells, In: H.J.Th. Goos, R.K. Rastogi, H. Vaudry, R. Pierantoni, Perspective in Comparative Endocrinology: Unity and Diversity. Monduzzi Editore S.p.A. - Medimond Inc., 2001, 827-833.


Research postgraduate student: CHU Chun Sing
Ph.D. thesis - Flow Cytometric Analysis of Cell-Cycle Regulatory Proteins During Apoptosis

Research postgraduate student: GU Peili

Ph.D. thesis - Molecular Studies of the CHH/MIH/GIH Neuropeptide Gene Family in Sand Shrimp, Metapenaeus Ensis

Research postgraduate student: HE Meizi

Ph.D. thesis - Body Composition, Energy Metabolism and Weight Reduction in Obese Chinese Women

Research postgraduate student: HE Mulan

Ph.D. thesis - Molecular Studies of Two Functional Gonadotropin-Releasing Hormone Receptors in Goldfish, Carassius Auratus

Research postgraduate student: HUANG Junchao

Ph.D. thesis - Molecular Phylogeny and Genetic Diversity of Sweetpotato (Ipomoea Batatas) and Its Wild Relatives

Research postgraduate student: LI Chi Hang, Jonathan

Ph.D. thesis - Characterization of a Sertoli Cell Product, Rat Myotubularin: Its Involvement in Cell-Cell Interactions in the Testis

Research postgraduate student: MRUK, Dolores Dorothy

Ph.D. thesis - A Study on the Dynamics of Sertoli-Germ Cell Interactions: New Perspectives on Male Fertility Control

Research postgraduate student: NGAN Sau Wai, Elly

Ph.D. thesis - Transcriptional Regulation of the Human Gonadotropin Releasing Hormone Receptor Gene

Research postgraduate student: XI Zhiqun

Ph.D. thesis - An Investigation on the Flavor Compounds and Texture in Chinese Chicken Meat

Research postgraduate student: CHU Mei Sze

MPhil. thesis - Biphasic Growth Hormone Release Induced by Protein Kinase C Activation in Grass Carp Pituitary Cells

Research postgraduate student: HO Po Ki

MPhil. thesis - Transcriptional Regulation of the Human Secretin Receptor Gene Expression

Research postgraduate student: IP Ying Chi

MPhil. thesis - Structure and Transcriptional Regulation of The Rabbit Sex Hormone-Binding Globulin Gene

Research postgraduate student: KEE Francis

MPhil. thesis - Aspartic Acid Scanning Mutation Analysis of a Receptor Isolated from Goldfish Specific to the Growth Hormone Releasing Hormone Salmon-Like Peptide

Research postgraduate student: LAU Lee Man

MPhil. thesis - Amino Acids in Soy Sauce

Research postgraduate student: LEE Shuk Man

MPhil. thesis - Cloning and Characterization of a Calmodulin Gene in Rice, Oryza Sativa

Research postgraduate student: MA Chi Him, Eddie

MPhil. thesis - Molecular Studies of Gonadotropin Releasing Hormone Receptors and Estrogen Receptors in Goldfish (Carassius Auratus)

Research postgraduate student: MO Chi Wai

MPhil. thesis - Prevention and Therapy of Infectious Bursal Disease by Molecular Approaches

Research postgraduate student: SAMY, Eileen Teresa

MPhil. thesis - Regulation of Testin and Prostaglandin D2 Synthetase Expression in Sertoli Cells: A Molecular and Cell Biology Study and Its Implication in Sertoli-Germ Cell Interactions

Research postgraduate student: SO Shun Han, Henry

MPhil. thesis - Studies on Morphological and Biochemical Changes of Epithelial Cells of the Gill of the Japanese Eel, Anguilla Japonica (Temminck & Schlegel), In Response to Chronic pH Changes

Research postgraduate student: TANG Kwan Yi

MPhil. thesis - Nutritional Status of Hospitalized Geriatrics and the Effects of Branched-Chain Amino Acids Supplementation on Pressure Sore Healing

Research postgraduate student: TSE Kei Yin

MPhil. thesis - A Study on the Hospital Food Service System in Hong Kong

Research postgraduate student: TSE Lai Yin

MPhil. thesis - Identification of a Novel PHI Receptor in Goldfish Carassius Auratus: Implications of Conservation of PHI Structure in Vertebrates

Research postgraduate student: WAI Sen Mun

MPhil. thesis - A Survey on Isozyme Genetics of the Domestic Chicken (Gallus Domesticus)

Research postgraduate student: WONG Kit Man

MPhil. thesis - Part I: Isolation and Characterization of the Highly Repetitive Sequences from Escherichia Coli and Their Uses in DNA Fingerprinting Part II: Molecular Characterization and Initial Development of a DNA Vaccine Against the Hog Cholera Virus

Research postgraduate student: WONG Yee Man

MPhil. thesis - Malnutrition in Hospitalized Geriatrics: A Pilot Study

Research postgraduate student: WU Hing Wan

MPhil. thesis - Characterization and Regulation of Vascular Endothelial Growth Factor Receptors Expression in the Testis

Research postgraduate student: YU Mei Kuen

MPhil. thesis - The Chloride Cell of the Gill Epithelium of Japanese Eel, Anguilla Japonica: Adaptation in Response to Salinity Change



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