DEPARTMENT OF BOTANY

Researchers:
Bao J. Cai Y. Chen S.F. Ching K.M. Chye M.L. Corke H.
Fung K.L. He H. Kong K.F. Lai J. Li H. Li H.
Ma C.Y. Ma Y.N. Meng G. Mock W.Y. Ouyang X. Siu N.C.
Sze J. Tsang J.S.H. Wai K.M. Wang L. Weerakoon S.K. Wen Z.
Xing J. Xu Z. Yap C.Y. Yau C.P. Ye X. Yeung E.C.T.
Yip W.K. Zee S.S.Y. Zhang Z.


Researcher : Bao J

List of Research Outputs

Bao J., Sun M. and Corke H., Analysis of genetic behavior of some starch properties in Indica rice (Oryza sativa L.): thermal properties, gel texture and swelling volume, Theoretical and Applied Genetics. Germany, 2002, 104: 408-413.
Bao J. and Corke H., Pasting properties of gamma-irradiated rice starches as affected by pH, Journal of Agricultural and Food Chemistry. USA, 2002, 50: 336-341.
Bao J., Cai Y. and Corke H., Prediction of rice starch quality parameters by near-infrared reflectance spectroscopy, Journal of Food Science. USA, 2001, 66: 936-939.


Researcher : Cai Y

List of Research Outputs

Bao J., Cai Y. and Corke H., Prediction of rice starch quality parameters by near-infrared reflectance spectroscopy, Journal of Food Science. USA, 2001, 66: 936-939.
Cai Y., Sun M., Schliemann W. and Corke H., Chemical stability and colorant properties of betaxanthin pigments from Celosia argentea, Journal of Agricultural and Food Chemistry>. USA, 2001, 49: 4429-4435.
Cai Y. and Corke H., Effect of postharvest treatments on Amaranthus betacyanin degradation evaluated by visible/near infrared spectroscopy, Journal of Food Science. USA, 2001, 66: 1112-1118.
He H., Cai Y., Sun M. and Corke H., Extraction and purification of squalene from Amaranthus grain, Journal of Agricultural and Food Chemistry. USA, 2002, 50: 368-372.


Researcher : Chen SF

Project Title:Enhanced production of high-value carotenoids by applying oxidative stresses to the cultures of green microalgae
Investigator(s):Dr. Chen S.F., Mr. Hou W.W., Prof. Hu Q., Dr. Jiang Y., Prof. Xiang W.Z.
Department:Botany
Source(s) of Funding:RGC Fundable Projects (Block Grant Funded)
Start Date:November 2001
Abstract:
To screen microalgae (Haematococcus, Chlorococcum, etc.) for potential of secondary carotenoid formation; to grow the selected microalgae in shake flasks and subsequently in fermenters by providing suitable nutrients including organic carbon sources to the cultures under heterotrophic and mixotrophic conditions; to apply various oxidative stresses to induce the formation of the secondary carotenoids.


Project Title:Study on the cell wall of the green microalga Haematococcus pluvialis (volvocales, chlorophyceae)
Investigator(s):Dr. Chen S.F., Prof. Hu Q.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:November 2001
Abstract:
To understand the formation of the cell wall of H. pluvialis under normal and environmental stress conditions.


List of Research Outputs

Chen H., Chen S.F., Chiu F.C.K. and Lo C.M.Y., The effect of yeast elicitor on the growth and secondary metabolism of hairy root cultures of Salvia miltiorrhiza, Enzyme and Microbial Technology. 2001, 28: 100-105.
Chen S.F. and Jiang Y., Algae and their Biotechnological Potential. Dordrecht, the Netherlands, Kluwer Academic Publishers, 2001, 316pp.
Chen S.F., Development of bioprocess engineering in China, The IFT Annual Meeting and Food Expo 2002. Anaheim, CA, USA (Abstract published at the IFT website). 2002.
Fan K.W., Chen S.F., Jones E.B.G. and Vrijmoed L.L.P., Docosahexaenoic acid (DHA) production from mangrove thaustochytrids, their physiology and food waste utilization potential, Proceedings of the International Conference on Essential Fatty Acids and Human Nutrition and Health, Shanghai, China, p. 192. 2002.
Fan W.K., Chen S.F., Jones E.B.G. and Vrijmoed L.L.P., Eicosapentaenoic acid and docosahexaenoic acid production and okara uilizing potential of thraustochytrids, Journal of Industrial Microbiology & Biotechnology. 2001, 27(4): 199-202.
Jiang Y., Chen S.F. and Li H., Effects of nitrogen source and vitamin B12 on docosahexaenoic acid production by Crypthecodinium cohnii, Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 69-78.
Li H., Jiang Y. and Chen S.F., Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after separation, Journal of Agricultural and Food Chemistry. 2002, 50: 1070-1072.
Li H. and Chen S.F., Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatography. In: Chen, F. & Jiang, Y. (eds), Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 127-134.
Li H. and Chen S.F., Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography, Journal of Chromatography A. 2001, 925: 133-137.
Li H. and Chen S.F., Preparative isolation and purification of salidroside from the Chinese medicinal plant Rhodiola sacchalinensis by high-speed counter-current chromatography, Journal of Chromatography A. 2001, 932: 91-95.
Li H., Lai J., Jiang Y. and Chen S.F., Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography, Journal of Chromatography A. 2002, 943(2): 235-239.
Li H. and Chen S.F., Preparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography, Journal of Chromatography A. 2001, 925: 109-114.
Li H. and Chen S.F., Simultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detection, Journal of Separation Science. 2001, 24: 271-274.
Li H. and Chen S.F., Simultaneous determination of twelve water- and fat-solutable vitamins by high-performance liquid chromatography with diode array detection, Chromatographia. 2001, 54: 270-273.
Liu X., Yuan J.P., Chung C.K. and Chen S.F., Cultivation technology of Haematococcus pluvialis in seawater and physiological functions of astaxanthin, Science and Technology of Food Industry. 2001, 129: 72-73.
Ma R.Y.N. and Chen S.F., Induction of astaxanthin formation in the green microalga Chlorococcum sp. by reactive oxygen species (ROS) under mixotrophic conditions of growth. In: Chen, F. & Jiang, Y. (eds)., Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 121-126.
Shi X.M. and Chen S.F., High yield production of lutein by heterotrophic Chlorella protothecoides in fed-batch systems. In: Chen, F. & Jiang, Y. (eds), Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 107-120.
Wen Z. and Chen S.F., A perfusion-cell bleeding strategy for enhancing the productivity of eicosapentaenoic acid in the culture of Nitzschia laevis, Applied Microbiology and Biotechnology. 2001, 57: 316-322.
Wen Z. and Chen S.F., Application of statistically-based experimental designs for optimizing eicosapentaenoic acid production by Nitzschia laevis. In: Chen, F. & Jiang, Y. (eds), Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 33-54.
Wen Z. and Chen S.F., Application of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevis, Biotechnology and Bioengineering. 2001, 75(2): 159-169.
Wen Z. and Chen S.F., Continuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: physiological study and process optimization, Biotechnology Progress. 2002, 18: 21-28.
Wen Z., Jiang Y. and Chen S.F., High cell density culture of the diatom Nitzchia laevis for eicosapentaenoic acid production: fed-batch development, Process Biochemistry. 2002, 37: 1447-1453.
Wen Z. and Chen S.F., Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis, Enzyme and Microbial Technology. 2001, 29: 341-347.
Wen Z. and Chen S.F., Optimization of nitrogen sources for the production of eicosapentaenoic acid by the diatom Nitzschia laevis in heterotrophic cultures. In: Chen, F. & Jiang, Y. (eds), Algae and Their Biotechnological Potential. The Netherlands, Kluwer academic Publishers, 2001, pp. 55-68.
Yap C.Y. and Chen S.F., Polyunsaturated fatty acids: biological significance, biosynthesis, and production by microalgae and microalgae-like organisms. In: Chen, F. & Jiang, Y. (eds)., Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 1-32.
Yuan J.P., Chen S.F., Liu X. and Li X.Z., Carotenoid composition in the green microalga Chlorococcum, Food Chemistry. 2002, 76(3): 319-325.
Zhong J.J., Chen H. and Chen S.F., Production of rosmarinic acid, lithospermic acid B, and tanshinones by suspension cultures of Ti-transformed Salvia miltiorrhiza cells in bioreactors, Journal of Plant Biotechnology. 2001, 3(2): 107-112.


Researcher : Ching KM

List of Research Outputs

Ching K.M. and Ma C.Y., Thermal aggregation of Dolichos lablab globulin (Abstract), Institute of Food Technologists Annual Meeting (Anaheim, CA). 2002.


Researcher : Chye ML

Project Title:Molecular characterization of ACBP2 in Arabidopsis
Investigator(s):Dr. Chye M.L.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1999
Abstract:
To demonstrate the functional role of the acyl-CoA binding domain of ACBP2 in binding acyl-CoA esters; to examine the expression of ACBP2 mRNA; to localize the ACBP2 protein using anti-ACBP2 polyclonal antibodies.


Project Title:Production of fungus-resistant transgenic potato
Investigator(s):Dr. Chye M.L.
Department:Botany
Source(s) of Funding:Plant and Fungal Biotechnology Area of Excellence
Start Date:March 2000
Abstract:
To produce fungus-resistant transgenic potato.


Project Title:Functional analysis of Arabidopsis acyl-CoA binding proteins
Investigator(s):Dr. Chye M.L.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 2000
Abstract:
To elucidate the function of Arabidopsis ACBP1 and ACBP2. Findings from this study could potentially lead to the manipulation of plant lipid transport/storage in oilseed crops.


Project Title:Expression of 3-hydroxy-3-methylglutarylCoA synthase in Brassica juncea
Investigator(s):Dr. Chye M.L.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:November 2000
Completion Date:October 2002
Abstract:
To understand the role of 3-hydroxy-3-methylglutarylCoA synthase in fungal protection and in flower/seed development in Brassica juncea.


Project Title:Characterization of Brassica Juncea 3-hydroxy-3-methylglutaryl-CoA synthase
Investigator(s):Dr. Chye M.L., Dr. Bach T.J.
Department:Botany
Source(s) of Funding:France/Hong Kong Joint Research Scheme
Start Date:January 2001
Completion Date:December 2002
Abstract:
To identify the amino acid residues in Brassica juncea HMG-CoA synthase (HMGS) essential for enzyme activity by site-directed in vitro mutagenesis.


Project Title:Regulation of cysteine proteinase expression in Solanum melongena
Investigator(s):Dr. Chye M.L., Dr. Yao K.M.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 2001
Abstract:
To elucidate the molecular basis for ethylene- induction and circadian regulation of SmCP, a gene encoding cysteine proteinase that functions at senescence and developmental events associated with programmed cell death (PCD) in Solanum melongena (brinjal/ eggplant/ aubergine).


Project Title:Investigations on the chitin-binding domains of BjCHI1
Investigator(s):Dr. Chye M.L.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:February 2002
Abstract:
To purify BjCHI1 from transgenic tobacco for in vitro investigations of agglutination assays to further understand the role of the two chitin-binding domains in plant-pathogen interactions.


List of Research Outputs

Li H. and Chye M.L., Membrane localization of Arabidopsis acyl-CoA binding protein ACBP2, 15th International Symposium on Plant Lipids, Okazaki, Japan. May 12-17, 2002.
Li H. and Chye M.L., Membrane localization of Arabidopsis acyl-CoA binding protein ACBP2, Xth International Congress of Plant Tissue Culture & Biotechnology, Orlando, USA, June 23-28,2002.
Xu Z.F., Qi W.Q., Ouyang X., Yeung E.C.T. and Chye M.L., A proteinase inhibitor II of Solanum americanum is expressed in phloem, Plant Molecular Biology. Netherlands, Kluwer Academic Publishers, 2001, 47: 727-738.


Researcher : Corke H

Project Title:A co-operative venture in food science education with the American Association of Cereal Chemists
Investigator(s):Dr. Corke H.
Department:Botany
Source(s) of Funding:Leung Kau Kui Research and Teaching Endowment Fund
Start Date:February 1995
Abstract:
Initiate a programme of international short courses in food sciece in Hong Kong; initiate an English to Chinese food science textbook translation programme.


Project Title:The diversity of cereal grain products in Hong Kong and southern China
Investigator(s):Dr. Corke H.
Department:Botany
Source(s) of Funding:Woo Ting Sang Agricultural Development Research Fund
Start Date:January 1996
Abstract:
The cereal industry in this region is incredibly diverse. There is a vast expansion of diversity fueled by increasing consumer affluence in China, and by the need for convenience products. What attributes of the cereal grain raw materials are most relaated to quality of these products? What additives and modified starches will be in most demand in future? Without basic scientific analysis of products on the market, these questions cannot be answered.


Project Title:The genetic basis of color development in wheat dough
Investigator(s):Dr. Corke H.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1997
Abstract:
To identify genetic resources of hexaploid wheat which lack the functional enzymes causing discoloration of dough, and to characterize the color stability of doughs formed from these variants.


Project Title:Genotypic differences in wheat starch properties related to food product quality
Investigator(s):Dr. Corke H., Dr. Abeysekera R., Prof. Robards A.W.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1998
Abstract:
1) To analyze genetic variation in wheat starch properties of selected genotypoes under a wide range of potential processing conditions (pH and salt levels), with and without added propylene glycol alginate; and 2) to use the results to elucidate the mecchanism of oil content reduction and texture changes, in steamed-and-fried instant noodles (SFIN). The key issues are a) the current inability of manufacturers to full control oil content; b) the recent introduction of oil-reducing additives on the mar ket, the effects of which are poorly understood; c) the desirability of developing a greater fundamental understanding of this product, so that predictive modelling of desired product changes will be feasible.


Project Title:Coix (Job's Tears) - a traditional grain with applications in functional foods
Investigator(s):Dr. Corke H.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:July 1999
Abstract:
To study the genetic variation in physicochemical properties of starch of normal and waxy coix genotypes, it is important in exploring new product development with coix as the base for functional (nutraceutical) foods with desirable texture profiles.


Project Title:Development and applications of betacyanin pigments from Amaranthus
Investigator(s):Dr. Corke H., Mr. Cai Y.Z., Dr. Phillips D.L., Dr. Sun M.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1999
Abstract:
We propose a multidisciplinary approach to the development of Amaranthus vegetative tissue betacyanin pigments as an economically viable food-grade colorant. We will investigate the agricultural production of high pigment varieties, establish methods too improve extraction of the pigments, determine factors influencing the color and stability of the pigments, and test various food uses for them.


Project Title:Resistant starch for controlled fermentation of sorghum-based alcoholic beverages
Investigator(s):Dr. Corke H.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:February 2001
Abstract:
To identify diversity in physical properties of starch, particularly in starch digestibility; to identify appropriate sources of genetic diversity in these properties, and controlling environmental diversity; to develop starch resources for enhanced quality of sorghum beer and liquor.


List of Research Outputs

Bao J., Sun M. and Corke H., Analysis of genetic behavior of some starch properties in Indica rice (Oryza sativa L.): thermal properties, gel texture and swelling volume, Theoretical and Applied Genetics. Germany, 2002, 104: 408-413.
Bao J. and Corke H., Pasting properties of gamma-irradiated rice starches as affected by pH, Journal of Agricultural and Food Chemistry. USA, 2002, 50: 336-341.
Bao J., Cai Y. and Corke H., Prediction of rice starch quality parameters by near-infrared reflectance spectroscopy, Journal of Food Science. USA, 2001, 66: 936-939.
Beta T. and Corke H., Genetic and environmental variation in sorghum starch properties, Journal of Cereal Science. UK, 2001, 34: 261-268.
Cai Y., Sun M., Schliemann W. and Corke H., Chemical stability and colorant properties of betaxanthin pigments from Celosia argentea, Journal of Agricultural and Food Chemistry. USA, 2001, 49: 4429-4435.
Cai Y. and Corke H., Effect of postharvest treatments on Amaranthus betacyanin degradation evaluated by visible/near infrared spectroscopy, Journal of Food Science. USA, 2001, 66: 1112-1118.
Chong C.K., Xing J., Phillips D.L. and Corke H., Development of NMR and Raman spectroscopic methods for the determination of the degree of substitution of maleate in modified starches, Journal of Agricultural and Food Chemistry. 2001, 49: 2702-2708.
He H., Cai Y., Sun M. and Corke H., Extraction and purification of squalene from Amaranthus grain, Journal of Agricultural and Food Chemistry. USA, 2002, 50: 368-372.
Tan Y.F., Sun M., Xing Y.Z., Hua J.P., Sun X.L., Zhang Q. and Corke H., Mapping quantitative trait loci for milling quality, protein content and color characteristics of rice using a recombinant inbred line population derived from an elite rice hybrid, Theoretical and Applied Genetics. Germany, 2001, 103: 1037-1045.
Tan Y.F., Xing Y.Z., Zhang Q. and Corke H., Quantitative genetic basis of gelatinization temperature of rice, Cereal Chemistry. USA, 2001, 78: 666-674.
Zhang Z. and Corke H., Trypsin inhibitor activity in vegetative tissue of sweet potato plants and its response to heat treatment, Journal of the Science of Food and Agriculture. United Kingdom, 2001, 81: 1358-1363.


Researcher : Fung KL

List of Research Outputs

Fung K.L., Purification of Brassica juncea Chitinase BjChI1 from Transgenic Tobacco (MPhil thesis). 2001.


Researcher : He H

List of Research Outputs

He H., Cai Y., Sun M. and Corke H., Extraction and purification of squalene from Amaranthus grain, Journal of Agricultural and Food Chemistry. USA, 2002, 50: 368-372.


Researcher : Kong KF

List of Research Outputs

Guan Y., Peiris J.S.M., Kong K.F., Dyrthing K.C., Ellis T.M., Sit T., Zhang L. and Shortridge K.F., H5N1 Influenza Viruses Isolated from Geese in Southeastern China: Evidence for Genetic Reassortment and Interspecies Transmission to Ducks, Virology. Elsevier Science, 2002, 292: 16-23 (2002).


Researcher : Lai J

List of Research Outputs

Li H., Lai J., Jiang Y. and Chen S.F., Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography, Journal of Chromatography A. 2002, 943(2): 235-239.


Researcher : Li H

List of Research Outputs

Li H. and Chye M.L., Membrane localization of Arabidopsis acyl-CoA binding protein ACBP2, 15th International Symposium on Plant Lipids, Okazaki, Japan. May 12-17, 2002.
Li H. and Chye M.L., Membrane localization of Arabidopsis acyl-CoA binding protein ACBP2, Xth International Congress of Plant Tissue Culture & Biotechnology, Orlando, USA, June 23-28,2002.
Li H. and Chen S.F., Simultaneous determination of nine water-soluble vitamins in pharmaceutical preparations by high-performance liquid chromatography with diode array detection, Journal of Separation Science. 2001, 24: 271-274.


Researcher : Li H

List of Research Outputs

Jiang Y., Chen S.F. and Li H., Effects of nitrogen source and vitamin B12 on docosahexaenoic acid production by Crypthecodinium cohnii, Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 69-78.
Li H., Jiang Y. and Chen S.F., Isolation and purification of lutein from the microalga Chlorella vulgaris by extraction after separation, Journal of Agricultural and Food Chemistry. 2002, 50: 1070-1072.
Li H. and Chen S.F., Preparative isolation and purification of astaxanthin from the green microalga Chlorococcum sp. by high-speed counter-current chromatography. In: Chen, F. & Jiang, Y. (eds), Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 127-134.
Li H. and Chen S.F., Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp. by high-speed counter-current chromatography, Journal of Chromatography A. 2001, 925: 133-137.
Li H. and Chen S.F., Preparative isolation and purification of salidroside from the Chinese medicinal plant Rhodiola sacchalinensis by high-speed counter-current chromatography, Journal of Chromatography A. 2001, 932: 91-95.
Li H., Lai J., Jiang Y. and Chen S.F., Preparative isolation and purification of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography, Journal of Chromatography A. 2002, 943(2): 235-239.
Li H. and Chen S.F., Preparative isolation and purification of six diterpenoids from the Chinese medicinal plant Salvia miltiorrhiza by high-speed counter-current chromatography, Journal of Chromatography A. 2001, 925: 109-114.
Li H. and Chen S.F., Simultaneous determination of twelve water- and fat-solutable vitamins by high-performance liquid chromatography with diode array detection, Chromatographia. 2001, 54: 270-273.


Researcher : Ma CY

Project Title:Structure-function study of oat globulin
Investigator(s):Prof. Ma C.Y., Dr. Mine Y.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:October 1998
Abstract:
To study the molecular basis of protein functionality using oat globulin as a model.


Project Title:Study of structure-function relationship in globulin from three indigenous Chinese legumes
Investigator(s):Prof. Ma C.Y.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:October 1999
Abstract:
To purify and isolate the major globulin protein fractions, legumin and vicilin, from the legume seeds and to conduct a detailed structural analysis; to measure the physicochemical and functional properties of the legume globulins and globulin fractions; to study thermal denaturation, aggregation and gelation of the legume protein fractions by thermal analysis, vibrational spectroscopy, microscopy and several rheological methods.


Project Title:Structural characterization of buckwheat seed proteins
Investigator(s):Prof. Ma C.Y.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:May 2001
Completion Date:April 2002
Abstract:
To isolate and purify the lysine and methionine rich major 280 kD globulin and 26kD basic subunit from common buckwheat, and to conduct a detailed structural analysis.


Project Title:Structure-function study of rice seed proteins
Investigator(s):Prof. Ma C.Y.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:December 2001
Abstract:
To isolate and purify the four major Osborne protein fractions from rice seeds, and to conduct a detailed structural analysis of the proteins and their major subfractions; to study the physicochemical and thermal denaturation, aggregation and gelation properties of the rice storage proteins.


List of Research Outputs

Ching K.M. and Ma C.Y., Thermal aggregation of Dolichos lablab globulin (Abstract), Institute of Food Technologists Annual Meeting (Anaheim, CA). 2002.
Li-Chan E.C.Y. and Ma C.Y., Thermal analysis of flaxseed (Linum usitatissimum) proteins by differential scanning calorimetry, Food Chemistry. 2002, 77: 495-502.
Ma C.Y., Asian Foods: Science and Technology (Book review), Food Research International. 2002, 35: 409-410.
Ma C.Y. and Phillips D.L., FT-Raman spectroscopy and its applications in cereal science, Cereal Chemistry. 2002, 79: 171-177.
Ma C.Y. and Weerakoon S.K., Study of rice globulin conformation by FTIR spectroscopy (Abstract), 224th American Chemical Society National Meeting (Boston, MA). 2002.
Ma C.Y., Study of thermal aggregation and gelation of oat globulin by Raman spectroscopy (Invited paper), First International Conference on Biomedical Spectroscopy (Cardiff, Wales, UK). 2002.
Meng G. and Ma C.Y., Fourier-transform infrared spectroscopic study of globulin from Phaseolus angularis (red bean), International Journal of Biological Macromolecules. 2001, 29: 287-294.
Meng G. and Ma C.Y., Thermal gelation of globulin from Phaseolus angularis (red bean), Food Research International. 2002, 35: 377-385.
Siu N.C., Ma C.Y., Mock W.Y. and Mine Y., Functional properties of oat globulin modified by a calcium-independent microbial transglutaminase, Journal of Agricultural and Food Chemistry. 2002, 50: 2666-2672.
Siu N.C., Ma C.Y. and Mine Y., Physicochemical and structural properties of oat globulin polymers formed by a microbial transglutaminase, Journal of Agricultural and Food Chemistry. 2002, 50: 2660-2665.


Researcher : Ma YN

List of Research Outputs

Ma Y.N., Physiological Responses of Chlorococcum SP. to External Stresses (PhD thesis). 2001.


Researcher : Meng G

List of Research Outputs

Meng G., Study of Structure-Function Relationships in Globulin from Phaseolus angularis (Red Bean) Seeds (PhD thesis). 2001.


Researcher : Mock WY

List of Research Outputs

Siu N.C., Ma C.Y., Mock W.Y. and Mine Y., Functional properties of oat globulin modified by a calcium-independent microbial transglutaminase, Journal of Agricultural and Food Chemistry. 2002, 50: 2666-2672.


Researcher : Ouyang X

List of Research Outputs

Xu Z.F., Qi W.Q., Ouyang X., Yeung E.C.T. and Chye M.L., A proteinase inhibitor II of Solanum americanum is expressed in phloem, Plant Molecular Biology. Netherlands, Kluwer Academic Publishers, 2001, 47: 727-738.


Researcher : Siu NC

List of Research Outputs

Siu N.C., Enzymatic Modification of Oat Globulin by Microbial Transglutaminase (MPhil thesis). 2001.


Researcher : Sze J

List of Research Outputs

Tsang J.S.H. and Sze J., Sec-dependent and Sec-independent translocation of haloacid dehalogenase Chd1 of Burkholderia cepacia MBA4 in Escherichia coli, FEMS Microbiology Letters. 2002, 211: 259-264.


Researcher : Tsang JSH

Project Title:Analysis of the regulation of dehalogenase IVa gene of Burkholderia cepacia MBA4
Investigator(s):Dr. Tsang J.S.H.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:December 2000
Abstract:
To investigate the regulation of hdlIVa. Molecular biology techniques will be used to unravel the regulatory elements of the gene and the regulatory factor(s) identified. Understanding how the hdlIVa gene is regulated could provide information for the development of a biosensor detection system for halogenated pollutants.


Project Title:Modernization of the teaching environment for molecular biology related courses
Investigator(s):Dr. Tsang J.S.H.
Department:Botany
Source(s) of Funding:Run Run Shaw Research and Teaching Endowment Fund
Start Date:January 2002
Abstract:
To modernize the provision of apparatus and equipment in oder to provide more hands-on training for undergraduate students.


Project Title:Purification of a haloacid dehalogenase from continuous culture of Burkholderia cepacia MBA4
Investigator(s):Dr. Tsang J.S.H.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:February 2002
Abstract:
To isolate and purify the dehalogenase expressed only in the continuous culture condition so that some of the amino acids sequence could be determined.


List of Research Outputs

Pang B.C.M. and Tsang J.S.H., Mutagenic analysis of the conserved residues in dehalogenase IVa of Burkholderia cepacia MBA4, FEMS Microbiology Letters. 2001, 204: 135-140.
Tsang J.S.H., Analysis of the complementation of yeast cup1 by a water hyacinth metallothionein gene, Yeast. 2001, 18: S173.
Tsang J.S.H. and Sze J., Sec-dependent and Sec-independent translocation of haloacid dehalogenase Chd1 of Burkholderia cepacia MBA4 in Escherichia coli, FEMS Microbiology Letters. 2002, 211: 259-264.
Wong P.H.Y. and Tsang J.S.H., Heterologous expression of a recombinant water hyacinth metallothionein gene in Saccharomyces cerevisiae, Yeast. 2001, 18: S292.


Researcher : Wai KM

List of Research Outputs

Wai K.M., Purification and Characterization of Beta-Cyanoalanine Synthase from Rice (Oryza sativa) (MPhil thesis). 2001.


Researcher : Wang L

List of Research Outputs

Zee S.S.Y., Wang L., Yau C.P., Yip W.K. and Yu X., Actin Visualization in Living Immature Pollen of Rice Using a GFP-Mouse Talin Fusion Protein, Acta Botanica Sinica. China, 2002, 44(6): 642-648.


Researcher : Weerakoon SK

List of Research Outputs

Ma C.Y. and Weerakoon S.K., Study of rice globulin conformation by FTIR spectroscopy (Abstract), 224th American Chemical Society National Meeting (Boston, MA). 2002.


Researcher : Wen Z

List of Research Outputs

Wen Z. and Chen S.F., A perfusion-cell bleeding strategy for enhancing the productivity of eicosapentaenoic acid in the culture of Nitzschia laevis, Applied Microbiology and Biotechnology. 2001, 57: 316-322.
Wen Z. and Chen S.F., Application of statistically-based experimental designs for optimizing eicosapentaenoic acid production by Nitzschia laevis. In: Chen, F. & Jiang, Y. (eds), Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 33-54.
Wen Z. and Chen S.F., Application of statistically-based experimental designs for the optimization of eicosapentaenoic acid production by the diatom Nitzschia laevis, Biotechnology and Bioengineering. 2001, 75(2): 159-169.
Wen Z. and Chen S.F., Continuous cultivation of the diatom Nitzschia laevis for eicosapentaenoic acid production: physiological study and process optimization, Biotechnology Progress. 2002, 18: 21-28.
Wen Z., Jiang Y. and Chen S.F., High cell density culture of the diatom Nitzchia laevis for eicosapentaenoic acid production: fed-batch development, Process Biochemistry. 2002, 37: 1447-1453.
Wen Z. and Chen S.F., Optimization of nitrogen sources for heterotrophic production of eicosapentaenoic acid by the diatom Nitzschia laevis, Enzyme and Microbial Technology. 2001, 29: 341-347.
Wen Z. and Chen S.F., Optimization of nitrogen sources for the production of eicosapentaenoic acid by the diatom Nitzschia laevis in heterotrophic cultures. In: Chen, F. & Jiang, Y. (eds), Algae and Their Biotechnological Potential. The Netherlands, Kluwer academic Publishers, 2001, pp. 55-68.
Wen Z., A High Yield and Productivity Strategy for Eicosapentaenoic Acid Production by the Diatom Nitzschia laevis in Heterotrophic Culture (PhD thesis). 2001.


Researcher : Xing J

List of Research Outputs

Chong C.K., Xing J., Phillips D.L. and Corke H., Development of NMR and Raman spectroscopic methods for the determination of the degree of substitution of maleate in modified starches, Journal of Agricultural and Food Chemistry. 2001, 49: 2702-2708.


Researcher : Xu Z

List of Research Outputs

Xu Z., Proteinase Inhibitor II from Solanum americanum: Molecular Characterization and Potential Use in Generating Insect-Resistant Transgenic Vegetables (PhD thesis). 2001.


Researcher : Yap CY

List of Research Outputs

Yap C.Y. and Chen S.F., Polyunsaturated fatty acids: biological significance, biosynthesis, and production by microalgae and microalgae-like organisms. In: Chen, F. & Jiang, Y. (eds)., Algae and Their Biotechnological Potential. The Netherlands, Kluwer Academic Publishers, 2001, pp. 1-32.


Researcher : Yau CP

List of Research Outputs

Yau C.P., Yu M.D., Zee S.S.Y. and Yip W.K., Isolation of a rice cDNA encoding a putative ethylene receptor, GenBank accession number AF460181. 2002.
Yau C.P., Zhuang C.X., Yip W.K. and Zee S.S.Y., Molecular characterization of a cDNA encoding putative pyruvate dehydrogenase kinase in rice, GenBank accession number AY026039. 2002.
Zee S.S.Y., Wang L., Yau C.P., Yip W.K. and Yu X., Actin Visualization in Living Immature Pollen of Rice Using a GFP-Mouse Talin Fusion Protein, Acta Botanica Sinica. China, 2002, 44(6): 642-648.


Researcher : Ye X

List of Research Outputs

Ye X., Yeung E.C.T. and Zee S.S.Y., Sperm movement during double fertilization of a flowering plant, Phaius tankervilliae, Planta. 2002, 215: 60-66.


Researcher : Yeung ECT

List of Research Outputs

Xu Z.F., Qi W.Q., Ouyang X., Yeung E.C.T. and Chye M.L., A proteinase inhibitor II of Solanum americanum is expressed in phloem, Plant Molecular Biology. Netherlands, Kluwer Academic Publishers, 2001, 47: 727-738.
Ye X., Yeung E.C.T. and Zee S.S.Y., Sperm movement during double fertilization of a flowering plant, Phaius tankervilliae, Planta. 2002, 215: 60-66.


Researcher : Yip WK

Project Title:Molecular cloning and physiological studies of the ethylene biosynthesis genes in rice and in wheat
Investigator(s):Dr. Yip W.K.
Department:Botany
Source(s) of Funding:Croucher Foundation
Start Date:December 1993
Abstract:
The aims of the project include (i) Molecualr cloning the cDNAs of ACC synthase and ACC oxidase in rice and in wheat; (ii) to test for the physiological relevant of the resulted clones; (iii) expression of the gene products; promoter analysis of the corresponding genomic genes; (iv) analysis of the functions of the genes in vivo in transgenic plants by incorporating these genes into the plant genome.


Project Title:Regulation of the plant HMG-CoA reductase kinase by reversible protein phosphorylation
Investigator(s):Dr. Yip W.K., Dr. Cheng H.C., Dr. Chye M.L.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
RGC Fundable Projects (Block Grant Funded)
Start Date:October 1996
Abstract:
To elucidate the signal transduction pathway in plant.


Project Title:The study of ethylene receptor genes on rice growth and development
Investigator(s):Dr. Yip W.K., Dr. Shiu O.Y.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 1999
Abstract:
To screen the rice genomic library for the ethylene receptor gene(s) and then obtain their DNA sequences; to investigate on the expression pattern of the ethylene receptor gene(s) in rice; to analyze the promoter sequence of the rice receptor genes usingg a transient expression system; to construct the sense and antisense receptor gene(s) in vector and transform it into rice cell in order to study the physiological responses of ethylene in transformed callus; to investigate the possibility of using the receptor gene(s) to affect male sterility in rice.


List of Research Outputs

Song S.Q. and Yip W.K., Effect of O2, ACC and CO2 concentration on the activity of partially purified ACC oxidase from papaya, Acta Physiologica Sinica. 2001, 27: 387-392.
Yau C.P., Yu M.D., Zee S.S.Y. and Yip W.K., Isolation of a rice cDNA encoding a putative ethylene receptor, GenBank accession number AF460181. 2002.
Yau C.P., Zhuang C.X., Yip W.K. and Zee S.S.Y., Molecular characterization of a cDNA encoding putative pyruvate dehydrogenase kinase in rice, GenBank accession number AY026039. 2002.
Zee S.S.Y., Wang L., Yau C.P., Yip W.K. and Yu X., Actin Visualization in Living Immature Pollen of Rice Using a GFP-Mouse Talin Fusion Protein, Acta Botanica Sinica. China, 2002, 44(6): 642-648.


Researcher : Zee SSY

Project Title:Studies on the control of flowering in Kumquat (Fortunella margarita)
Investigator(s):Prof. Zee S.S.Y.
Department:Botany
Source(s) of Funding:Woo Ting Sang Agricultural Development Research Fund
Start Date:August 1987
Abstract:
To investigate the effect of water stress on Kumquat flower production.


Project Title:The biotechnological development and conservation of ornamental plants
Investigator(s):Prof. Zee S.S.Y., Prof. Ye X.L.
Department:Botany
Source(s) of Funding:Croucher Foundation
Start Date:July 1992
Abstract:
To seek funding to expand the collaboration to include cultivation of ornamental plants by modern biotechnology techniques.


Project Title:To study the functional behavior of actin cystokeleton in living embyro sacs in Torenia fournieri before and during fertilization
Investigator(s):Prof. Zee S.S.Y.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:November 2000
Abstract:
To study the organization of the actin cytoskeleton in the embryo sac before and during fertilization (including the reception of the pollen tube, migration of male gametes and their fusion, and the migration of the endosperm nucleus after fertilization etc.).


Project Title:Establishment of a system to transfer multi-Pisti Genes into Saffron (a traditional Chinese medicine)
Investigator(s):Prof. Zee S.S.Y.
Department:Botany
Source(s) of Funding:Block Grant Earmarked for Research
Start Date:May 2001
Abstract:
To develop traditional Chinese medicine using transgenic plant and biotechnological techniques


Project Title:Visualization and characterization of actin and microtubule cytoskeletal organization in living pollen-mother-cells and microspores of male sterile rice using GFP-based markers
Investigator(s):Prof. Zee S.S.Y., Dr. Yau C.P.
Department:Botany
Source(s) of Funding:Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:September 2001
Abstract:
To investigate and systematically characterize the pattern of change and organization of actin and microtubule cytoskeleton in living pollen-mother-cells (and microspores) of male sterile rice (used in the three -line and two-line hybrid rice system of production in China) by employing a non-destructive visualization technique using expression of green fluorescence protein (GFP).


List of Research Outputs

Li Y., Zee S.S.Y., Liu Y., Huang B. and Yen L., Circular F-actin bundles and a G-actin gradient in pollen and pollen tubes of Lilium davidii, Planta. 2001, 213: 722-730.
Yau C.P., Yu M.D., Zee S.S.Y. and Yip W.K., Isolation of a rice cDNA encoding a putative ethylene receptor, GenBank accession number AF460181. 2002.
Yau C.P., Zhuang C.X., Yip W.K. and Zee S.S.Y., Molecular characterization of a cDNA encoding putative pyruvate dehydrogenase kinase in rice, GenBank accession number AY026039. 2002.
Ye X., Yeung E.C.T. and Zee S.S.Y., Sperm movement during double fertilization of a flowering plant, Phaius tankervilliae, Planta. 2002, 215: 60-66.
Yuan M., Fu Y., Wang F., Huang B., Zee S.S.Y. and Hepler P.K., Fertilization in Torenia fournieri: actin organization and nuclear behavior in the central cell and primary endosperm, Science in China. China, 2002, 45(2): 212-224.
Zee S.S.Y., Wang L., Yau C.P., Yip W.K. and Yu X., Actin Visualization in Living Immature Pollen of Rice Using a GFP-Mouse Talin Fusion Protein, Acta Botanica Sinica. China, 2002, 44(6): 642-648.
Zee S.S.Y., Liu X., Zhu H. and Lu Y., Further Studies on Microtubule Organizational Changes During Megagametogenesis in Rice Embryo Sac, Acta Botanica Sinica. 2001, 43(9): 910-917.
Zhu Z. and Zee S.S.Y., Cytoskeleton in pollen tube, in "Biology of Agiosperm Fertilization" (Ed. by Hu, S.Y. and Yang, H.Y.), Science Press. China, 2002, pages 29-58.


Researcher : Zhang Z

List of Research Outputs

Zhang Z., Nutritional Quality and Starch Physicochemical Properties in Sweetpotato (PhD thesis). 2001.


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