EYE INSTITUTE



Researcher : Chan CWS

List of Research Outputs

Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment. , British Journal Ophthalmology. 2009, In press.
Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment, British J Ophthalmology. 2010, 94(3): 311-8.


Researcher : Chan JCH

List of Research Outputs

Cheung J.J., Chan J.C.H., Pong C.F. and Lai J.S.M., Acute angle closure glaucoma, Ophthalmology. United States of America, 2009, 2039.
Chung C.F., Chan J.C.H., Wong E.W. and Lai J.S.M., Penetration of the orbit by a chopstick, Asian Journal of Ophthalmology. 2010.
Tham C.C., Lai J.S.M., Kwong Y.Y., Lam S.W., Chan J.C.H. and Chui T.Y., Correlation of previous acute angle-closure attack with extent of Correlation of previous acute angle closure attack with extent of synechial angle closure in chronic primary angle-closure glaucoma patients., Eye. United Kingdom, 2009, 920-923.
Tham C.C., Kwong Y.Y., Leung D.Y., Lam S.W., Li F.C., Chiu T.Y., Chan J.C.H. and Lai J.S.M., Phacoemulsification Versus Phacotrabeculectomy In Chronic Angle-closure Glaucoma With Cataract: Complications, Archives of Ophthalmology. United States of America, 2010, 303-311.


Researcher : Chan RYC

List of Research Outputs

Chan R.Y.C., Tang E.W.H. and Lai J.S.M., Angle configuration after argon laser peripheral iridoplasty and cataract extraction using anterior segment optical coherence tomography in acute phacomorphic angle closure, Asian Journal of Ophthalmology. 2010, 96-97.


Researcher : Choy BNK

List of Research Outputs

Choy B.N.K., Tang W.T. and Lai J.S.M., A case series contact lens-associated Fusarium keratitis in Hong Kong, Cornea. USA, 2009, 955.


Researcher : Chung CF

List of Research Outputs

Chung C.F. and Lai J.S.M., Enophthalmos caused by orbital venous malformation, Hong Kong Medical Journal. Hong Kong, 2009, 299-300.
Chung C.F., Chan J.C.H., Wong E.W. and Lai J.S.M., Penetration of the orbit by a chopstick, Asian Journal of Ophthalmology. 2010.


Researcher : Hon C

List of Research Outputs

Au W.Y., Hon C., Yau K., Lai W.W.K., Tam S. and Kwong Y.L., Two cases of monocular visual loss during oral arsenic trioxide therapy of acute promyelocytic leukemia., American Journal of Hematology. 2009, 84: 699.


Researcher : Lai JSM

Project Title:To compare the histological changes in the trabecular meshwork and iris between primary angle closure glaucoma and primary open angle glaucoma
Investigator(s):Lai JSM
Department:Eye Institute
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:12/2009
Abstract:
Purpose: To compare the trabecular meshwork and iris ultrastructures between primary angle closure glaucoma (PACG) and primary open angle glaucoma (POAG). Glaucoma is a sight-threatening eye disease in which the optic nerve degenerates in an abnormally fast rate. There are mainly 2 large groups of glaucoma, the PACG and the POAG. In the majority of the cases in these 2 groups, the intraocular pressure (IOP) is persistently raised and is the major contributing factor for the optic nerve degeneration. The cause of the increased IOP is mainly a result of a blockage in the drainage pathway of the aqueous humor at the angle. In PACG, the iris tissue adheres to the trabecular meshwork at the angle (angle closure) obstructing the outflow of the aqueous. In POAG, the angle remains open but there is ultrastructural changes in the trabecular meshwork resulting in obstruction of the aqueous outflow.1 Surgical treatment of the both PACG and POAG is by performing a filter operation, trabeculectomy to create an external opening for the drainage of the aqueous. In PACG, other surgical options are available to open up the closed angle by either a direct approach (lysis of the peripheral anterior synechiae) or by removing the cataract. In many of the cases, the IOP will show a significant decrease after these procedures.2,3 However, in some cases, despite reopening of the angle, the IOP failed to decrease. Sihota has shown evidence of loss of endothelial cells in the trabecular meshwork in PACG eyes.4 References: 1. Potau JM, Canals M, Costa J, et al. Ultrastructural characteristics of glaucomatous trabecular meshwork. Arch Soc Esp Oftalmol. 2000;75:21-28 2. Lai JS*, Tham CC, Chan JC. The clinical outcomes of cataract extraction by phacoemulsification in eyes with primary angle closure glaucoma (PACG) and co-existing cataract: a prospective case series. Journal of Glaucoma 2006;15:47-52 3. Lai JS*, Tham CC, Chua JK, Lam DS. The efficacy and safety of 180o goniosynechialysis followed by diode laser peripheral iridoplasty in the treatment of chronic angle-closure glaucoma. Journal of Glaucoma 2000; 9:388-391. 4. Sihota R, Lakshmaiah NC, Walia KB, et al. The trabecular meshwork in acute and chronic angle closure glaucoma. Indian J Ophthalmol. 2001;49:255-259


List of Research Outputs

Chan R.Y.C., Tang E.W.H. and Lai J.S.M., Angle configuration after argon laser peripheral iridoplasty and cataract extraction using anterior segment optical coherence tomography in acute phacomorphic angle closure, Asian Journal of Ophthalmology. 2010, 96-97.
Cheung J.J., Chan J.C.H., Pong C.F. and Lai J.S.M., Acute angle closure glaucoma, Ophthalmology. United States of America, 2009, 2039.
Choy B.N.K., Tang W.T. and Lai J.S.M., A case series contact lens-associated Fusarium keratitis in Hong Kong, Cornea. USA, 2009, 955.
Chung C.F. and Lai J.S.M., Enophthalmos caused by orbital venous malformation, Hong Kong Medical Journal. Hong Kong, 2009, 299-300.
Chung C.F., Chan J.C.H., Wong E.W. and Lai J.S.M., Penetration of the orbit by a chopstick, Asian Journal of Ophthalmology. 2010.
Ho C.L., Lai J.S.M., Aquino M.V., Rojanaponpun P., Wong H.T., Aquino M.C., Gerber Y., Belkin M. and Barkana Y., Selective laser trabeculoplasty for primary angle closure with persistently elevated intraocular pressure after iridotomy, Journal of Glaucoma. USA, 2009, 563-566.
Lai J.S.M., Cataract and Glaucoma, World Glaucoma Day. Hong Kong, 2010.
Lai J.S.M., Practical pearls: acute phacomorphic angle closure, Hong Kong College of Emergency Medicine. 2009.
Pong C.F. and Lai J.S.M., A patient with bilateral cataracta membranacea, Ophthalmic Surgery Laser And Imaging. United States of America, 2010, 1-2.
Tham C.C., Lai J.S.M., Kwong Y.Y., Lam S.W., Chan J.C.H. and Chui T.Y., Correlation of previous acute angle-closure attack with extent of Correlation of previous acute angle closure attack with extent of synechial angle closure in chronic primary angle-closure glaucoma patients., Eye. United Kingdom, 2009, 920-923.
Tham C.C., Kwong Y.Y., Leung D.Y., Lam S.W., Li F.C., Chiu T.Y., Chan J.C.H. and Lai J.S.M., Phacoemulsification Versus Phacotrabeculectomy In Chronic Angle-closure Glaucoma With Cataract: Complications, Archives of Ophthalmology. United States of America, 2010, 303-311.
Yeung J.C., Chung C.F. and Lai J.S.M., Orbital myiasis complicating squamous cell carcinoma of eyelid, Hong Kong Medical Journal. Hong Kong, 2010, 63-65.


Researcher : Lai WWK

Project Title:The use of a conjugate of mushroom (Agaricus bisporus) lectin and 5-fluorouracil as an inhibitry aget against proliferative vitreoretinopathy
Investigator(s):Lai WWK, Wong DSH
Department:Medical Faculty
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:12/2007
Abstract:
Rhegmatogenous retinal detachment (RRD) occurs when fluid from the vitreous cavity passes through a tear in the retina and results in the separation of the neurosensory retina from the underlying retinal pigment epithelium (RPE). Nearly all patients with a symptomatic RRD will progressively lose vision unless the detachment is surgically repaired. Once a RRD occurs the eye undergoes a modified healing process, or proliferative vitreoretinopathy (PVR), which involves cellular proliferation, tissue contraction and laying down of collagen. Although most cases of RRD can be successfully repaired by surgery, PVR is the cause of surgical failure in up to 15% of patients.In PVR, contraction of membranes that develop on both surfaces of the retina leads to retinal redetachment and surgical failure. Despite the increase in success rate with the introduction of sophisticated microsurgery, 25% of all initially successful PVR cases result in redetachment. Even in cases where the retina is reattached, the final visual results are often disappointing. Recent attempts by members of our group using adjunctive therapy during PVR surgery that consists of 5-fluorouracil (5-FU), an antimetabolite, and low molecular weight heparin, showed some promise in preventing PVR in patients who were at high risk of developing PVR, but not once the process had become established. It has been proposed that a higher concentration of 5-FU may be useful in established PVR by modulating the various cell types involved, but the dosage would exceed that could be tolerated by the RPE and photoreceptors. Thus, newer and targeted treatment is necessary that would allow delivery of an efficacious yet non-toxic concentration of 5-FU. De-differentiated RPE cells have emerged as key players in PVR. These cells behave like wound fibroblasts and therefore proliferate, migrate and adhere to extracellular matrix in order to generate the contractile forces in PVR tissue. Newer agents are thus required, not only as prophylaxis, but which address these key wound healing properties of RPE cells. Lectins are carbohydrate-binding proteins of non-immune origin that agglutinate cells or precipitate polysaccharides or glycoconjugates. The lectin of the edible mushroom, Agaricus bisporus (ABL), which binds the cell membrane TF antigen, is a reversible nontoxic inhibitor of epithelial cell proliferation. Recently, our group has shown this lectin to be antiproliferative to bovine15 and human RPE in vitro. Our group has recently synthesized a novel conjugate of ABL and 5-FU. It has been shown to be antiproliferative to RPE cells and without cytotoxicity. In this study, we will investigate the role of the conjugate on the wound healing properties of RPE cells in vitro. Specifically, we will examine whether the conjugate would modulate the proliferation, migration, adhesion, and contraction of RPE cells in vitro.


Project Title:Open label, prospective, double arm, randomized, pilot study to evaluate the safety and efficacy of the same day administration of PDT with verteporfin and an intravitreal injection of ranibizumab vs. ranibizumab monotherapy for the treatment of subfoveal CNV secondary to neovascular age related macular degeneration in the Asian population
Investigator(s):Lai WWK, Li KKW, Wong DSH, McGhee S, Lam CLK, Chan CWS
Department:Medical Faculty
Source(s) of Funding:Pharmaceutical Industry - General Award
Start Date:07/2008
Abstract:
To evaluate the safety and efficacy of same day administration of PDT with verteporfin and an intravitreal injection of ranibizumab vs. intravitreal ranibizumab monotherapy for the treatment of subfoveal CNV secondary to exudative AMD in the Asian population; to assess the quality of life and utility values of patients with exudative AMD before and after intravitreal injection of ranibizumab +/- PDT in Hong Kong.


Project Title:Production of Iris Pigment Epithelial Cells for transplantation to treat Age-Related Macular Degeneration
Investigator(s):Lai WWK, Lo ACY, Fletcher C, Wong DSH
Department:Eye Institute
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2009
Abstract:
1. To isolate iris pigment epithelial (IPE) cells from non-dystrophic rats 2. To optimize growth kinetics and investigate the characteristics of IPE cells 3. To evaluate a range of differentiation factors and growth factors that influence cell behavior by either enhancing or neutralizing specific growth factors, human serum levels and differentiating agents (e.g. retinoic acid) for RPE cell production from rat IPE cells 4. To measure a range of quantifiable changes in order to determine if cellular rejuvenation has been successful.


List of Research Outputs

Au W.Y., Hon C., Yau K., Lai W.W.K., Tam S. and Kwong Y.L., Two cases of monocular visual loss during oral arsenic trioxide therapy of acute promyelocytic leukemia., American Journal of Hematology. 2009, 84: 699.
Chan M.C.W., Chan W.Y., Yu C.L., Ho C.C., Yuen K.M., Fong J.H.M., Tang L.L.S., Lai W.W.K., Lo A.C.Y., Chui W.H., Sihoe A.D.L., Kwong D.L.W., Tsao G.S.W., Poon L.L.M., Guan Y., Nicholls J.M. and Peiris J.S.M., Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract, American Journal of Pathology. 2010, 176(4): 1828-40.
Chan W.Y., Yu C.L., Yuen K.M., Fong J.H.M., Lo A.C.Y., Lai W.W.K., Wong D.S.H., Nicholls J.M., Peiris J.S.M. and Chan M.C.W., Infection of influenza A (H5N1) virus in human eye epithelium, an in vitro and ex vivo study , The 28th Annual Meeting of the American Society of Virology. 2009.
Early Treatment for Retinopathy of Prematurity Cooperative Group .. and Lai W.W.K., Progression of type 2 to type 1 retinopathy of prematurity in the Early Treatment for Retinopathy of Prematurity Study, Archives of Ophthalmology. 2010, 128(4): 461-5.
Lai W.W.K., 3D Teaching Systems in Ophthalmology: Retina & Optic Nerve. World Ophthalmic Educational Colloquium 2010 , World Ophthalmology Congress, Berlin, Germany. 2010.
Lai W.W.K., Sarraf D., Chan R.V.P., Grover S. and Fiscella R.G., Age-related macular degeneration and mimicking macular lesions - an unique stereoscopic adventure!, American Academy of Ophthalmology Annual Meeting, San Francisco, USA. 2009.
Lai W.W.K., Age-related macular degeneration – who is at risk? , Frontiers in Biomedical Research, The University of Hong Kong. 2009.
Lai W.W.K., Age-related macular degeneration – who is at risk?, Frontiers in Biomedical Research, The University of Hong Kong, Hong Kong. 2009.
Lai W.W.K., Can we medically improve the structural outcome after successful surgical repair of retinal detachment? , Chinese Ocular Fundus Diseases Forum / Special Academic Congress of Chinese Ocular Fundus Disease, Shanghai, China. 2010.
Lai W.W.K., Oxane that floats?, Heavy Tamponade Meeting (HEATAM3), Amsterdam, Netherlands. 2009.
Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment. , British Journal Ophthalmology. 2009, In press.
Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment, British J Ophthalmology. 2010, 94(3): 311-8.
Ozurdex Geneva Study Group .. and Lai W.W.K., Randomized, sham-controlled trial of dexamethasone intravitreal implant in patients with macular edema due to retinal vein occlusion, Ophthalmology. 2010, 117(6): 1134-1146.


Researcher : Leung GYO

List of Research Outputs

Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment. , British Journal Ophthalmology. 2009, In press.
Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment, British J Ophthalmology. 2010, 94(3): 311-8.


Researcher : Li KKW

List of Research Outputs

Li K.K.W., Tang E.W.H., Li P.S.H. and Wong D.S.H., Double peel using triamcinolone acetonide and trypan blue in the management of myopic macular hole with retinal detachment: a case-control study. , Clin Experiment Ophthalmol. 2010. 2010.
Williams C.J., Lui W.C., Li K.K.W., Wong D.S.H. and Lo A.C.Y., Correspondence. author reply, Retina. 2010. 2010, 30(3): 534-5.


Researcher : Li PSH

List of Research Outputs

Li K.K.W., Tang E.W.H., Li P.S.H. and Wong D.S.H., Double peel using triamcinolone acetonide and trypan blue in the management of myopic macular hole with retinal detachment: a case-control study. , Clin Experiment Ophthalmol. 2010. 2010.


Researcher : Li SY

Project Title:Neuroscience 2009 Lutein, a xanthophyll, protects retinal ganglion cells from ischemia in vitro
Investigator(s):Li SY
Department:Eye Institute
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:10/2009
Completion Date:10/2009
Abstract:
N/A


List of Research Outputs

Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Beneficial effects of lycium barbarum polysaccharides on neurons and blood-retinal barrier in retinal ischemia/reperfusion injury, 5th International Symposium on Healthy Aging. 2010, Page 59.
Li S.Y. and Lo A.C.Y., Lutein Protects RGC-5 Cells Against Hypoxia and Oxidative Stress., Int J Mol Sci. 2010, 11: 2109-17.
Li S.Y., Yeung C.M., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Retina From Ischemia/reperfusion Injury, ARVO 2010 Annual Meeting. 2010.
Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Protection Of Retinal Neurons By Administration Of Lycium Barbarum (wolfberry) In A Murine Model Of Acute Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Li S.Y., The 6th Action for Vision Eye Foundation Young Research Award 2nd runner up, Action for Vision, Hong Kong. 2010.
Li S.Y., The 7th Action for Vision Eye Foundation Young Research Award 2nd runner up , Action for Vision, Hong Kong. 2010.
Lo A.C.Y., Fu Z., Li S.Y., Wong D.S.H. and Chung S.K., Genetic Deletion Of Aldose Reductase Protects The Neonatal Mouse Retina From Oxygen-induced Retinopathy, ARVO 2010 Annual Meeting. 2010.
Lo A.C.Y., Yeung C.M., Li S.Y., Chang R.C.C., So K.F. and Wong D.S.H., Neuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injury, Society for Neuroscience 2009. Program No. 150.13: Poster No. K2.
Wang H., Lau W.M., Yau S.Y., Li S.Y., Leung N.E.L.S.O.N., Wang N.I.N.G.L.I., Tang S.W., Lee T.M.C. and So K.F., The roles of paroxetine and corticosterone on adult mammalian retinal ciliary body cell proliferation, The Chinese Medical Journal. 2010, 123: 1305-1310.
Woo T.T.Y., Li S.Y., Lai W.W.K., Wong D.S.H. and Lo A.C.Y., Lutein Inhibition Of Photoreceptor Death In A Rat Model Of Retinal Detachment, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Yang D., Yeung C.M., Li S.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Brain From Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.


Researcher : Lo ACY

Project Title:Oxidative stress and neuronal dysfunction in retinopathy of prematurity
Investigator(s):Lo ACY, Chung SK, Wong DSH
Department:Eye Institute
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:05/2009
Abstract:
Retinopathy of prematurity (ROP) is a vasoproliferative retinal disorder that develops in preterm babies, subsequent to them being supported with supplemental oxygen. In ROP, there is initially an increase of retinal blood flow and oxygen delivery. Together with the restricted ability to inactivate reactive oxygen species (ROS), this leads to lipid peroxidation and impairment of ocular circulation, causing vessel constriction, endothelial cell degeneration and finally the typical ROP neo-vascularization. ROP can also result in retinal dysfunction, although the cellular basis for these deficits is unclear. It is generally believed that many illnesses in preterm infants, including ROP, are thought to be related to the action of ROS. Our previous work on retinal ischemia/reperfusion (I/R) showed that retinal neuronal degeneration was associated with increased apoptosis and oxidative stress [1]. In addition, genetic deletion or pharmacological inhibition of aldose reductase (AR), a rate limiting enzyme in the polyol pathway, prevented retinal ganglion cell loss and oxidative stress [1]. More importantly, administration of lutein, an antioxidant, could protect RGC as well as decreased oxidative stress in the injured retina [2], suggesting the importance of oxidative stress in retinal neuronal degeneration. The aim of this proposal is to clarify the role of oxidative stress in the pathogenesis of retinal neuronal injury induced by hyperoxia on a cellular basis and therefore development of ROP in preterm infants using a mouse model of ROP. The potential use of aldose reductase inhibition in preventing oxygen-induced retinopathy will also be explored. Besides neuronal dysfunction, neovascularization is also an important feature in ROP. Retinal neovascularization is thought to occur in response to a hypoxic insult (as a result of VEGF inhibition and reduced vessel growth due to high oxygen), which leads to changes in the existing vasculature and compensatory, albeit pathological, new capillary growth. Therefore, we also aim to determine the extent of neovascularization in our models of ROP upon AR deletion using AR knockout mice [3]. Objectives: 1. To investigate the involvement of oxidative stress in neuronal degeneration after oxygen induced retinopathy 2. To determine the contribution of aldose reductase to neuronal damage in an animal model of retinopathy of prematurity (ROP) Reference 1. Cheung, A.K., et al., Gene deletion and pharmacological inhibition of aldose reductase protect against retinal ischemic injury. Exp Eye Res, 2007. 2. Li, S.Y., et al., Lutein rescued retinal neurons and decreased oxidative stress in a model of acute retinal ischemia/reperfusion. Invest Ophthalmol Vis Sci, 2008. in press. 3. Ho, H.T., et al., Aldose reductase-deficient mice develop nephrogenic diabetes insipidus. Mol Cell Biol, 2000. 20(16): p. 5840-6. 4. Cheung, A.K., et al., Aldose reductase deficiency prevents diabetes-induced blood-retinal barrier breakdown, apoptosis, and glial reactivation in the retina of db/db mice. Diabetes, 2005. 54(11): p. 3119-25.


Project Title:Neuroscience 2009 Neuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injury
Investigator(s):Lo ACY
Department:Eye Institute
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:10/2009
Completion Date:10/2009
Abstract:
N/A


Project Title:Oxidative stress, neuronal dysfunction and neovascularization in retinopathy of prematurity
Investigator(s):Lo ACY, Wong DSH
Department:Eye Institute
Source(s) of Funding:Germany/Hong Kong Joint Research Scheme
Start Date:01/2010
Abstract:
Refer to hard copy


List of Research Outputs

Chan M.C.W., Chan W.Y., Yu C.L., Ho C.C., Yuen K.M., Fong J.H.M., Tang L.L.S., Lai W.W.K., Lo A.C.Y., Chui W.H., Sihoe A.D.L., Kwong D.L.W., Tsao G.S.W., Poon L.L.M., Guan Y., Nicholls J.M. and Peiris J.S.M., Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract, American Journal of Pathology. 2010, 176(4): 1828-40.
Chan W.Y., Yu C.L., Yuen K.M., Fong J.H.M., Lo A.C.Y., Lai W.W.K., Wong D.S.H., Nicholls J.M., Peiris J.S.M. and Chan M.C.W., Infection of influenza A (H5N1) virus in human eye epithelium, an in vitro and ex vivo study , The 28th Annual Meeting of the American Society of Virology. 2009.
Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Beneficial effects of lycium barbarum polysaccharides on neurons and blood-retinal barrier in retinal ischemia/reperfusion injury, 5th International Symposium on Healthy Aging. 2010, Page 59.
Li S.Y. and Lo A.C.Y., Lutein Protects RGC-5 Cells Against Hypoxia and Oxidative Stress., Int J Mol Sci. 2010, 11: 2109-17.
Li S.Y., Yeung C.M., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Retina From Ischemia/reperfusion Injury, ARVO 2010 Annual Meeting. 2010.
Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Protection Of Retinal Neurons By Administration Of Lycium Barbarum (wolfberry) In A Murine Model Of Acute Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Lo A.C.Y., Fu Z., Li S.Y., Wong D.S.H. and Chung S.K., Genetic Deletion Of Aldose Reductase Protects The Neonatal Mouse Retina From Oxygen-induced Retinopathy, ARVO 2010 Annual Meeting. 2010.
Lo A.C.Y., Neuroprotection in retinal and cerebral ischemia/reperfusion injury, School of Optometry, The Hong Kong Polytechnic University. 2010.
Lo A.C.Y., Yeung C.M., Li S.Y., Chang R.C.C., So K.F. and Wong D.S.H., Neuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injury, Society for Neuroscience 2009. Program No. 150.13: Poster No. K2.
Mi X., Chiu K., Van G., Leung J.W.C., Lo A.C.Y., Chung S.K., Chang R.C.C. and So K.F., The effect of lycium barbarum (wolfberry) on the expression of endothelin-1 and its receptors in an ocular hypertension model of glaucoma, 5th International Symposium on Healthy Aging. 2010, Page 55.
Williams C.J., Lui W.C., Li K.K.W., Wong D.S.H. and Lo A.C.Y., Correspondence. author reply, Retina. 2010. 2010, 30(3): 534-5.
Woo T.T.Y., Li S.Y., Lai W.W.K., Wong D.S.H. and Lo A.C.Y., Lutein Inhibition Of Photoreceptor Death In A Rat Model Of Retinal Detachment, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Yang D., Yeung C.M., Li S.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Brain From Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Yeung C.M., Lo A.C.Y., Cheung A.K.H., Chung S.S.M., Wong D.S.H. and Chung S.K., More severe type 2 diabetes-associated ischemic stroke injury is alleviated in aldose reductase-deficient mice, Journal of Neuroscience Research. 2010, 88: 2026-2034.


Researcher : Pong CF

List of Research Outputs

Cheung J.J., Chan J.C.H., Pong C.F. and Lai J.S.M., Acute angle closure glaucoma, Ophthalmology. United States of America, 2009, 2039.
Pong C.F. and Lai J.S.M., A patient with bilateral cataracta membranacea, Ophthalmic Surgery Laser And Imaging. United States of America, 2010, 1-2.


Researcher : Tang EWH

List of Research Outputs

Chan R.Y.C., Tang E.W.H. and Lai J.S.M., Angle configuration after argon laser peripheral iridoplasty and cataract extraction using anterior segment optical coherence tomography in acute phacomorphic angle closure, Asian Journal of Ophthalmology. 2010, 96-97.
Li K.K.W., Tang E.W.H., Li P.S.H. and Wong D.S.H., Double peel using triamcinolone acetonide and trypan blue in the management of myopic macular hole with retinal detachment: a case-control study. , Clin Experiment Ophthalmol. 2010. 2010.


Researcher : Tang WT

List of Research Outputs

Choy B.N.K., Tang W.T. and Lai J.S.M., A case series contact lens-associated Fusarium keratitis in Hong Kong, Cornea. USA, 2009, 955.


Researcher : Wong DSH

Project Title:Inhibition of provisional matrix synthesis in proliferative vitreoretinopathy (PVR) prevention
Investigator(s):Wong DSH, Lo ACY
Department:Medical Faculty
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2008
Abstract:
The research direction of our project has been changed and the new project title is: Chromosomal analysis in ocular adnexal lymphoma The ocular adnexal lymphomas (OAL) are malignant lymphoid neoplasms that develop in the orbit, conjunctiva, eyelid and lacrimal gland. They constitute less than 1% of all non-Hodgkin lymphoma (NHL), but represent 8% of those NHL occurring in extra-nodal sites[1]. According to the new WHO Classification[2] and the Revised European and American Lymphoma (R.E.A.L) Classification, 60% to 70% of all OAL are of extra-marginal zone B-cell lymphomas (EMZL) subtype. The less common subtypes of OAL include diffuse large B-cell (DLBCL) and follicular lymphoma (FL), each representing approximately 10% of the total OAL. In this project, we mainly focus on OAL of EMZL subtype; those OAL, such as FL, DLBCL and mantle cell lymphoma (MCL) are grouped together as “non-EMZL”. EMZL are low-grade B-cell lymphomas, which were first described in the stomach, and then subsequently in other extra-nodal sites e.g. lung, ocular adnexa, salivary and thyroid glands[3]. EMZL are thought to arise from neoplastic B-cells, which are located in the marginal zone of a secondary lymphoid follicle. This monoclonal B-cell population is comprised typically of centrocyte-like cells, monocytoid cells as well some with a plasmacellular differentiation. Infiltration of an adjacent epithelium or mucosa by the neoplastic B-cells has resulted in some of these tumors being termed as mucosa-associated lymphoid tissue (MALT) lymphomas[4]. The majority of the patients with OAL present with Stage IE tumors (Ann Arbor staging), and are usually treated with low-dose radiotherapy. The clinical course of these tumors is an indolent one, characterised by recurrent tumor occurring at other extranodal sites, including the contralateral ocular adnexa [5]. It is difficult to predict both histomorphologically and clinically, in which patient the NHL will recur or possibly spread systemically. Some prognostic markers have been suggested on the basis of large cohorts [6-8], and include serum lactate dehydrogenase, age and gender of the patient, EMZL versus non-EMZL tumors, and the Ki-67 growth fraction of the neoplastic B-cells [9, 10]. EMZL demonstrate several cytogenetic alterations. These include trisomies 3 and 18, and the translocations t(11;18)(q21;q21), t(1;14)(p22;q32), t(14;18)(q32;q21) and t(3;14)(p13;q32)[11-13]. Most of these cytogenetic alterations are linked with a dysregulation of of the BCL10 and MALT1 genes, which in turn affect a common signaling mechanism, the NF-kB signaling pathway. Dysregulation of the NF-kB pathway results in an uncontrolled proliferation of lymphocytes [12, 13]. EMZL at different locations show distinctive cytogenetic mutation profiles[13], and EMZL OAL is particularly related with t(11;18) and t(14;18) translocations, 10% and 7%, respectively [12]. Recently reported loss of TNFAIP3 and A20, both at 6q23, are associated with the pathogenesis of EMZL of the ocular adnexa via enhanced activation of the NF-kB signaling mechanism [14, 15]. To date, it has not been determined whether any of these chromosomal abnormalities are of clinical prognostic significance. Although cytogenetic mutations of EMZL have been studied in detail, little research has been performed on the hypermethylation status of tumor suppressor genes in OAL. Chromosomal methylation involves the addition of methyl groups on the deoxyribonucleotide base cytosine, specifically at Cytosine-Guanine (CG)-rich sites, i.e. CpG islands in the promoter regions. Such hypermethylation can prevent the binding of transcription factors and lead to down-regulation of the expression of the affected gene. Hypermethylation of tumor suppressor genes is a common epigenetic phenomenon that leads to such “gene silencing”, and plays an important role in development of many tumors, including lymphomas. Hypermethylation status of various genes in different subtypes of lymphoma was studied, such as p16, p15, DAP-kinase, androgen receptor, SHP1, MLH1 and MGMT have shown hypermethylation, and the levels of methylation can be variable among different subtypes [16-19]. Many studies have showed a correlation between EMZL pathogenesis and microorganism infection. Helicobacter pylori infection is well known to be associated with gastric EMZL development: growth of the neoplastic B-cells can be stimulated by intratumoral H. pylori specific T-cells [12, 13, 20]. Several recent studies have shown Chlamydia psitacci is present in some ocular adnexal EMZL [21-23]. The correlation between C. psitacci and ocular adnexal EMZL is, however, variable among different geographical regions, being most frequently in Italy (80%), followed by Germany (47%), the East Coast of USA (35%) and the Netherlands (29%)[21, 22]. As yet there is no evidence showing microbial DNA contributing to EMZL OAL pathogenesis[24]. In this project, our aims include: - To investigate whether hypermethylation pattern in OAL EMZL and compare it with the reactive lymphoproliferative lesions of the OAL as well as with the OAL non-EMZL - To investigate whether hypermethylation pattern in OAL EMZL is similar (or not) to that of EMZL in other locations, such as gastric and pulmonary EMZL - To correlate methylation status with anatomical location within the ocular adnexa using the novel TNM-based staging system for OAL [25], and with the subsequent clinical course in a well-defined cohort of OAL - To assess what the functional result of hypermethylation of any genes in OAL: e.g. subsequent dysregulation of signalling pathways - To assess whether the methylation pattern of the OAL EMZL may be related to the presence or absence of microorganisms, such as Chlamydia psitacci infection. Similarly, we plan to correlate the methylation status in stomach EMZL with the clinical history with and without Helicobacter pylori infection.


Project Title:To introduce bevacizumab for the treatment of macular degeneration and diabetic retinopathy to Queen Mary Hospital
Investigator(s):Wong DSH, Lai WWK, Li KKW
Department:Medical Faculty
Source(s) of Funding:S.K. Yee Medical Foundation - General Award
Start Date:03/2008
Abstract:
To introduce this new treatment to public patients; to use the drug appropriately in the patients best interest; to demonstrate that a high quality service can be delivered effectively and cost effectively.


Project Title:To develop a tool that can be used to study patients with eye diseases in Hong Kong, namely a translated Chinese version of the established Visual Function Questionnaire (VFQ-25)
Investigator(s):Wong DSH, Lai WWK, McGhee S, Lam CLK, Chan CWS
Department:Medical Faculty
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:07/2008
Abstract:
To develop a tool that can be used to study patients with eye diseases in Hong Kong, namely a translated Chinese version of the established Visual Function Questionnaire (VFQ-25)


Project Title:The Royal College of Ophthalmologists Annual Congress 2009 Can Lutein Protect the Outer Retina?
Investigator(s):Wong DSH
Department:Medical Faculty
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:05/2009
Abstract:
N/A


List of Research Outputs

Chan W.Y., Yu C.L., Yuen K.M., Fong J.H.M., Lo A.C.Y., Lai W.W.K., Wong D.S.H., Nicholls J.M., Peiris J.S.M. and Chan M.C.W., Infection of influenza A (H5N1) virus in human eye epithelium, an in vitro and ex vivo study , The 28th Annual Meeting of the American Society of Virology. 2009.
Kenawy N., Wong D.S.H., Stappler T., Romano M.R., Das R.A. and Hebbar G., Does the presence of an epiretinal membrane alter the cleavage plane during internal limiting membrane peeling?, Ophthalmology. 2010. 2009, 117(2): 320-3 e1.
Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment. , British Journal Ophthalmology. 2009, In press.
Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment, British J Ophthalmology. 2010, 94(3): 311-8.
Li K.K.W., Tang E.W.H., Li P.S.H. and Wong D.S.H., Double peel using triamcinolone acetonide and trypan blue in the management of myopic macular hole with retinal detachment: a case-control study. , Clin Experiment Ophthalmol. 2010. 2010.
Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Beneficial effects of lycium barbarum polysaccharides on neurons and blood-retinal barrier in retinal ischemia/reperfusion injury, 5th International Symposium on Healthy Aging. 2010, Page 59.
Li S.Y., Yeung C.M., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Retina From Ischemia/reperfusion Injury, ARVO 2010 Annual Meeting. 2010.
Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Protection Of Retinal Neurons By Administration Of Lycium Barbarum (wolfberry) In A Murine Model Of Acute Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Lo A.C.Y., Fu Z., Li S.Y., Wong D.S.H. and Chung S.K., Genetic Deletion Of Aldose Reductase Protects The Neonatal Mouse Retina From Oxygen-induced Retinopathy, ARVO 2010 Annual Meeting. 2010.
Lo A.C.Y., Yeung C.M., Li S.Y., Chang R.C.C., So K.F. and Wong D.S.H., Neuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injury, Society for Neuroscience 2009. Program No. 150.13: Poster No. K2.
Mehta J., Farnell D.J., Stappler T., Liazos E. and Wong D.S.H., Perception of tilt following counter-rotation surgery., Clin Experiment Ophthalmol. 2010. 2010, 38(3):: 284-91.
Romano M.R., Gibran S.K., Marticorena J., Wong D.S.H. and Heimann H., Can an intraoperative bevacizumab injection prevent recurrent postvitrectomy diabetic vitreous hemorrhage?, Eur J Ophthalmol.. 2009, 19(4): 618-21.
Romano M.R., Stappler T., Marticorena J., Groenewald C., Pearce I., Gibran S.K., Wong D.S.H. and Heimann H., Primary vitrectomy with Densiron-68 for rhegmatogenous retinal detachment., Graefes Arch Clin Exp Ophthalmol.. 2009.
Sheridan C.M., Pate S., Hiscott P., Wong D.S.H., Pattwell D.M. and Kent D., Expression of hypoxia-inducible factor-1alpha and -2alpha in human choroidal neovascular membranes., Graefes Arch Clin Exp Ophthalmol. . 2009, 247(10): 1361-7.
Stappler T., Williams R. and Wong D.S.H., F4H5: a novel substance for the removal of silicone oil from intraocular lenses., Br J Ophthalmol 2010. 2010, 94(3): 364-7.
Williams C.J., Lui W.C., Li K.K.W., Wong D.S.H. and Lo A.C.Y., Correspondence. author reply, Retina. 2010. 2010, 30(3): 534-5.
Williams R.L., Day M., Garvey M.J., English R. and Wong D.S.H., Increasing the extensional viscosity of silicone oil reduces the tendency for emulsification., Retina. 2010. 2010, 30(2): 300-4.
Wishart P.K., Choudhary A. and Wong D.S.H., Ahmed glaucoma valves in refractory glaucoma: a 7-year audit, Br J Ophthalmol. 2010. 2009.
Woo T.T.Y., Li S.Y., Lai W.W.K., Wong D.S.H. and Lo A.C.Y., Lutein Inhibition Of Photoreceptor Death In A Rat Model Of Retinal Detachment, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Yang D., Yeung C.M., Li S.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Brain From Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Yeung C.M., Lo A.C.Y., Cheung A.K.H., Chung S.S.M., Wong D.S.H. and Chung S.K., More severe type 2 diabetes-associated ischemic stroke injury is alleviated in aldose reductase-deficient mice, Journal of Neuroscience Research. 2010, 88: 2026-2034.


Researcher : Woo TTY

List of Research Outputs

Woo T.T.Y., Li S.Y., Lai W.W.K., Wong D.S.H. and Lo A.C.Y., Lutein Inhibition Of Photoreceptor Death In A Rat Model Of Retinal Detachment, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.


Researcher : Yau K

List of Research Outputs

Au W.Y., Hon C., Yau K., Lai W.W.K., Tam S. and Kwong Y.L., Two cases of monocular visual loss during oral arsenic trioxide therapy of acute promyelocytic leukemia., American Journal of Hematology. 2009, 84: 699.


Researcher : Yeung CM

Project Title:The effects of aldose reductase deficiency on retinal ischemic injury in diabetic db/db mice
Investigator(s):Yeung CM, Lo ACY, Wong DSH
Department:Eye Institute
Source(s) of Funding:Small Project Funding
Start Date:04/2009
Completion Date:09/2009
Abstract:
The research direction of our project has been changed and the new project title is: The effects of Lycium barbarum polysaccharides on brain and retinal ischemic injuries in mice. Retinopathy is a common complication of diabetes. People with diabetic retinopathy also have increased risks of other systemic vascular complications such as stroke. As the disease progresses, regional failure of the microvascular circulation will lead to retinal ischemia. Retinal ischemic injury is therefore also common in patients with diabetes. Lycium barbarum, a Traditional Chinese Medicine, is well known for its beneficial effects (including nourishing the liver and kidney as well as improving the eyesight) in human. It has been previously shown that the extracts of Lycium barbarum exhibit cytoprotective effects on a number of different cell types including neurons. More recently, its neuroprotective effects on retinal ganglion cells have also been demonstrated. Based on these earlier studies, the aim of our present study is to evaluate the effects of Lycium barbarum polysaccharides (LBP) on brain and retinal ischemic injuries in order to further investigate its therapeutic potential in stroke and diabetic retinopathy. By using an in vivo mouse model of brain and retinal ischemia, the objectives of the present proposal are: 1.) To determine the neuroprotective effects of LBP on brain and retinal ischemic injuries 2.) To determine the potential therapeutic dosage(s) and time point(s) of LBP treatment 3.) To determine the possible mechanism(s)/pathway(s) involved in the neuroprotection by LBP


List of Research Outputs

Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Beneficial effects of lycium barbarum polysaccharides on neurons and blood-retinal barrier in retinal ischemia/reperfusion injury, 5th International Symposium on Healthy Aging. 2010, Page 59.
Li S.Y., Yeung C.M., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Retina From Ischemia/reperfusion Injury, ARVO 2010 Annual Meeting. 2010.
Li S.Y., Yeung C.M., Yu W.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Protection Of Retinal Neurons By Administration Of Lycium Barbarum (wolfberry) In A Murine Model Of Acute Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.
Lo A.C.Y., Yeung C.M., Li S.Y., Chang R.C.C., So K.F. and Wong D.S.H., Neuroprotective effects of Lycium barbarum polysaccharides on ischemic stroke injury, Society for Neuroscience 2009. Program No. 150.13: Poster No. K2.
Yang D., Yeung C.M., Li S.Y., Chang R.C.C., So K.F., Wong D.S.H. and Lo A.C.Y., Lycium Barbarum Polysaccharides Protects Brain From Ischemia/reperfusion Injury, Joint Annual Scientific Meeting of the Hong Kong Society of Neurosciences and the Biophysical Society of Hong Kong. 2010.


Researcher : Yeung IYL

List of Research Outputs

Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment. , British Journal Ophthalmology. 2009, In press.
Lai W.W.K., Leung G.Y.O., Chan C.W.S., Yeung I.Y.L. and Wong D.S.H., Simultaneous spectral domain OCT and fundus autofluorescence imaging of the macula and microperimetric correspondence after successful repair of rhegmatogenous retinal detachment, British J Ophthalmology. 2010, 94(3): 311-8.


Researcher : Yu CS

Project Title:Fat tissue engineering for facial recontouring
Investigator(s):Yu CS, Wong DSH, Lo ACY, Chan BP
Department:Eye Institute
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:09/2009
Abstract:
Changes in the orbital rim and the anterior maxilla markedly influence facial appearance, including the appearance of the eyes. The key to rejuvenate the periorbital region is volume restoration through reinflation and recontouring. Similarly, posttraumatic scars, lipoatrophy and hemifacial atrophy all represent clinical situations in which augmentation of facial soft tissues is necessary to restore premorbid contour. To address these needs, a variety of filler compounds including hyaluronic acid, collagen, silicone, and autologous fat have been promoted as potential remedies for correction of volume loss. Although several limitations have been identified with each of these substances, autologous fat transfer may represent the most promising filler presently available given its accessibility and nonallergenic nature. Despite recent refinement and growing standardization of injecting aspirated fat techniques, longevity of transplanted fat remains a pivotal concern. Although restoration of contour can be reproducibly achieved, lasting results one or more years after injection remain in question. In particular, mobile areas of the face such as the glabella have been notoriously unresponsive to long-term correction with fat injection1,2. The poor results of free-fat autotransplantation are thought to be attributed to the mature fat cells’ low tolerance of ischemia and the slow rate of revascularization of the grafts3. Magnetic resonance imaging performed before and after fat transfer in 53 patients demonstrated greater than 50% volume loss 6 months after injection4. Eremia and Newman1 evaluated short- and long-term results in 116 patients undergoing autologous fat grafting to the face and found that whereas short-term results at 3 to 4 months were uniformly excellent, gradual volume loss could be appreciated with longer follow-up such that, at 14 months, only 4% of patients maintained correction. Other regions, including the forehead and infraorbital area, have shown more variable results2,5. Considering the current limitation to autologous fat transfer, novel modalities have been increasingly investigated to meet ongoing demands for soft-tissue augmentation and/or reconstruction in the face. Using multipotent cells with the capacity to not only self-replicate but also produce tissue-forming progenitor cells may engender the potential to address various deficiencies associated with autologous fat transfer. Several studies have already shown the ability for mesenchymal cells, whether harvested from bone marrow or fat, to possess the in vitro ability to expand in culture and differentiate into adipose tissue forming units6-8. Such cells have also been exploited to promote in vivo fat formation in rats8. A biodegradable carrier with specific requirements toward material and structure is also needed, which allows these cells to invade and differentiate after transplantation. Mechanical stability of the carrier is important, and it should not be resorbed too quickly after transplantation. The three-dimensional structure of a scaffold carrying mesenchymal cells is also important for the deposition of extracellular matrix for the substitute to achieve properties similar to those of the native tissue 9,10. The objective of this study is to investigate the use of collagen-based carrier for fat cell delivery. The porous collagen matrix can support cellular ingrowth and new extracellular matrix synthesis. Collagen-based carrier also provides a good matrix to fibroblasts that fully differentitate and exhibit normal morphology and metabolisim in vivo11.


Project Title:Establishment of oculoplastic service at Queen Mary Hospital
Investigator(s):Yu CS
Department:Eye Institute
Source(s) of Funding:S.K. Yee Medical Foundation - General Award
Start Date:02/2010
Abstract:
n/a


List of Research Outputs

Yu C.S., Update on Management of Thyroid Eye Disease., International Healthy Aging Symposium 2010. . 2010.


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