| THE
UNIVERSITY OF HONG KONG |
DEPARTMENT OF BOTANY
| Researcher : Beta T |
| List of Research Outputs |
| Beta T. and Corke H., Effect of ferulic acid and catechin on starch pasting properties, Cereal Chemistry. 2004, 81: 418-422. |
| Researcher : Cai Y |
| List of Research Outputs |
| Cai Y., Luo Q., Sun M. and Corke H., Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer, Life Sciences. 2004, 74: 2157-2184. |
| Cai Y., Corke H. and Li W., Buckwheat, In: "Encyclopedia of Grain Science", (C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 1: Pages 120-128. |
| Cai Y., Corke H. and Wu H.X., Amaranthus, In: "Encyclopedia of Grain Science", (C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 1: Pages 1-10. |
| Corke H. and Cai Y., Grain production and consumption - Asia, In:"Encyclopedia of Grain Science",(C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 2: Pages 77-86. |
| Researcher : Chen SF |
| Project Title: | Genetic manipulation of carotenoid biosynthesis in Chlamydomonas reinhardtii for production of astaxanthin |
| Investigator(s): | Dr. Chen S.F. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 01/2003 |
| Completion Date: | 12/2003 |
| Abstract: |
| To genetically manipulate carotenoid biosynthesis in the green microalga chlamydomonas reinhardtii so as to produce the red ketocarotenoid astaxanthin; to develop an optimal growth system for high-yield production of astaxanthin by the engineered organism; to construct plasmids containing single crtO, crtZ or psy genes under the control of a synthetic inducible promoter and construct a bi-directional polar promoter for directing the expression of crtO, crtZ and psy; to introduce the gene crtO and/or crtZ coupled with psy into C. reinhardtii; to investigate the expression of transgenes in C. reinhardtii; to investigate growth kinetics and productivity of astaxanthin of the C. reinhardtii mutants under various culture conditions. |
| Project Title: | Transgenic chlamydomonas reinhardtii as an experimental system to study astaxanthin biosynthesis in green microalgae |
| Investigator(s): | Dr. Chen S.F., Dr. Huang J.C., Prof. Sandmann G. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2003 |
| Abstract: |
| To construct plasmids contianing single bkt (b-carotene ketolase), and bhy (b-carotene hydroxylase) under the control of a synthetic inducible promoter, and construct a bi-directional polar promoter for directing the expression of bkt and bhy; to introduce the gene bkt and/or bhy into C. reinhardtii; to investigate the expression of transgenes in C. reinhardtii; to study the molecular aspects of astaxanthin biosysthesis; and to investigate growth kinetics and productivity of astaxanthin of the Chlamydomonas mutants under photoautotrophic, mixotrophic and heterotrophic culture conditions. |
| Project Title: | Transgenic chlamydomonas reinhardtii as an experimental system to study astaxanthin biosynthesis in green microalgae |
| Investigator(s): | Dr. Chen S.F., Dr. Huang J.C., Prof. Sandmann G. |
| Department: | Botany |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 09/2003 |
| Abstract: |
| To construct plasmids contianing single bkt (b-carotene ketolase), and bhy (b-carotene hydroxylase) under the control of a synthetic inducible promoter, and construct a bi-directional polar promoter for directing the expression of bkt and bhy; to introduce the gene bkt and/or bhy into C. reinhardtii; to investigate the expression of transgenes in C. reinhardtii; to study the molecular aspects of astaxanthin biosysthesis; and to investigate growth kinetics and productivity of astaxanthin of the Chlamydomonas mutants under photoautotrophic, mixotrophic and heterotrophic culture conditions. |
| Project Title: | Enhanced biosynthesis of astaxanthin in the green microalga Chlorella zofingiensis |
| Investigator(s): | Dr. Chen S.F. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2003 |
| Abstract: |
| To investigate the biosynthesis of the ketocarotenoid astaxanthin in mixotrophic or heterotrophic culture of the microalga C. zofingiensis in which glucose and nitrate are manipulated to facilitate high-yield production of the pigment. |
| Project Title: | New antiparasitic molecules from microalgae |
| Investigator(s): | Dr. Chen S.F., Prof. Vivares C. |
| Department: | Botany |
| Source(s) of Funding: | France/Hong Kong Joint Research Scheme |
| Start Date: | 01/2004 |
| Abstract: |
| To obtain new antiparasitic molecules from microalgae from natural sources. |
| List of Research Outputs |
| Chen S.F., High-yield production of valuable microalgal products: nutrient, bioreactor and process considerations. Abstracts and Program, 5th Asia-Pacific Conference on Algal Biotechnology, 18-21 October 2003. Qingdao, China, pp. 18-19. |
| Chen S.F., Matching high-value products and low-cost processes: the potential of microalgal biotechnology, International Symposium on Bioprocess and Biomolecular Engineering, 15-17 December 2003. Shanghai, China, pp. 50-51. |
| Chen S.F., Lu H.T. and Jiang Y., Purification of paeoniflorin from Paeonia lactiflora Pall. by high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1040(2): 205-208. |
| Huang J. and Chen S.F., Differential expression of three b-carotene ketolase genes in a unicellular chlorophyte. Abstracts and Program, 5th Asia-Pacific Conference on Algal Biotechnology, 18-21 October 2003. Qingdao, China, pp. 132. |
| Ip P.F. and Chen S.F., Enhanced production of a high-value carotenoid astaxanthin by the green microalga Chlorella zofingiensis with ROS and RNS, Conference Handbook, Great Science! Great Food! 20-23 June 2004. Hamilton, New Zealand, p. 41. |
| Jiang Y., Fan K.W., Wong R.T.Y. and Chen S.F., Fatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei, Journal of Agricultural and Food Chemistry. 2004, 52: 1196-1200. |
| Jiang Y., Lu H.T. and Chen S.F., Preparative purification of glycyrrhizin extracted from the root of liquorice using high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1033(1): 183-186. |
| Lai J., Jiang Y., He X.W., Huang J. and Chen S.F., Separation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheres, Journal of Chromatography B. 2004, 804(1): 25-30. |
| Lai J., He X.W. and Chen S.F., Separation and determination of the antitumor drug piritrexim by molecularly imprinted microspheres in high performance liquid chromatography, Analytical and Bioanalytical Chemistry. 2003, 377(1): 208-213. |
| Li H., Jiang Y. and Chen S.F., Separation and purification of bioactive components from the Chinese medicinal plant Scutellaria baicalensis, Eighth International People-Plant Symposium 4-6 June, Awaji Island. Hyogo, Japan, 2004, Programme and Abstracts: pp. 39-40. |
| Liang S.Z., Liu X.M., Chen S.F. and Chen Z.J., Current microalgal health food R & D activities in China, Hydrobiologia. 2004, 512: 45-48. |
| Liu X. and Chen S.F., Cell differentiation and colony alteration of Nostoc flagelliforme, an edible terrestrial cyanobacterium, in different liquid suspension cultures, Folia Microbiologica. 2003, 48(5): 619-626. |
| Liu X., Chen S.F. and Jiang Y., Differentiation of Nostoc flagelliforme and its neighboring species using fatty acid profiling as a chemotaxonomic tool, Current Microbiology. 2003, 47(6): 467-474. |
| Lu H.T., Jiang Y. and Chen S.F., Application of high-speed counter-current chromatography to the preparative separation and purification of baicalin from the Chinese medicinal plant Scutellaria baicalensis, Journal of Chromatography A. 2003, 1017: 117-123. |
| Lu H.T., Jiang Y. and Chen S.F., Application of preparative high-speed counter-current chromatography for separation of chlorogenic acid from Flos Lonicerae, Journal of Chromatography A. 2004, 1026(1-2): 185-190. |
| Lu H.T., Jiang Y. and Chen S.F., Determination of squalene using high-performance liquid chromatography with diode array detection, Chromatographia. 2004, 59: 367-371. |
| Lu H.T., Jiang Y. and Chen S.F., Preparative high-speed counter-current chromatography for purification of shikonin from the Chinese medicinal plant Lithospermum erythrorhizon, Journal of Chromatography A. 2004, 1023(1): 159-163. |
| Wang S.B., Hu Q., Sommerfeld M. and Chen S.F., An optimized protocol for isolation of soluble proteins from microalgae by two-dimensional gel electrophoresis analysis, Journal of Applied Phycology. 2003, 15: 485-496. |
| Wang S.B., Hu Q., Sommerfeld M. and Chen S.F., Cell wall proteomics of the green alga Haematococcus pluvialis (Chlorophyceae), Proteomics. 2004, 4: 692-708. |
| Wang S.B., Hu Q., Sommerfeld M. and Chen S.F., Proteomic analysis of molecular response to oxidative stress of the green alga Haematococcus pluvialis (chlorophyceae). Abstracts and Program, 5th Asia-Pacific Conference on Algal Biotechnology, 18-21 October 2003. Qingdao, China, pp. 137. |
| Wen Z. and Chen S.F., Heterotrophic production of eicosapentaenoic acid by microalgae, Biotechnology Advances. 2003, 21(4): 273-294. |
| Researcher : Choi SM |
| List of Research Outputs |
| Choi S.M., Zhao Y. and Ma C.Y., Raman spectroscopic study of buckwheat globulin basic subunit (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Choi S.M., Zhao Y. and Ma C.Y., Study of heat-induced aggregation of buckwheat globulin basic subunit (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Researcher : Chung YKW |
| List of Research Outputs |
| Wong H.P.S., Chung Y.K.W. and Tsang J.S.H., Identification of the regulatory element of dehalogenase IVa gene of Burkholderia cepacia MBA4, In Abstracts of the XIX International Congress of Genetics. Melbourne, Australia, 2003, pp. 185. |
| Researcher : Chye ML |
| Project Title: | Production of fungus-resistant transgenic potato |
| Investigator(s): | Dr. Chye M.L. |
| Department: | Botany |
| Source(s) of Funding: | Areas of Excellence Scheme (UGC) |
| Start Date: | 03/2000 |
| Abstract: |
| To produce fungus-resistant transgenic potato. |
| Project Title: | Functional analysis of Arabidopsis acyl-CoA binding proteins |
| Investigator(s): | Dr. Chye M.L. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2000 |
| Abstract: |
| To elucidate the function of Arabidopsis ACBP1 and ACBP2. Findings from this study could potentially lead to the manipulation of plant lipid transport/storage in oilseed crops. |
| Project Title: | Regulation of cysteine proteinase expression in Solanum melongena |
| Investigator(s): | Dr. Chye M.L., Dr. Yao K.M. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2001 |
| Abstract: |
| To elucidate the molecular basis for ethylene- induction and circadian regulation of SmCP, a gene encoding cysteine proteinase that functions at senescence and developmental events associated with programmed cell death (PCD) in Solanum melongena (brinjal/ eggplant/ aubergine). |
| Project Title: | Subcellular localization and mutational analysis of Brassica juncea HMGS |
| Investigator(s): | Dr. Chye M.L., Dr. Bach T.J. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2002 |
| Abstract: |
| The project attempts to localize BjHMGS by using: 1) anti-BjHMGS antibodies in western blot analysis and immunoelectron microscopy; and 2) BjHMGS-Green Fluorescent Protein fusions. The research team will also identify the amino acid residues in BhHMGS that are essential for catalytic activity by site-directed in vitro mutagenesis. This would be a prerequisite to the crystallization of BjHMGS and the elucidation of its 3-D structure and interaction with substrates, inhibitors and other factors affecting its biological activity. |
| Project Title: | Investigations on Arabidopsis ACBP3 |
| Investigator(s): | Dr. Chye M.L. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 01/2003 |
| Completion Date: | 12/2003 |
| Abstract: |
| To elucidate the function of a putative acyl-CoA binding protein, ACBP3, in Arabidopsis thaliana that my laboratory has identified from the Arabidopsis genome sequence; to establish that ACBP3 binds acyl-CoA esters, thus proving it is an acyl-CoA binding protein; to study the pattern of ACBP3 expression in order to elucidate its role in the plant. |
| Project Title: | Analysis of the catalytic domain in Brassica chitinase |
| Investigator(s): | Dr. Chye M.L., Prof. Mowbray S. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2003 |
| Completion Date: | 10/2004 |
| Abstract: |
| To elucidate the amino acids that are crucial for activity at the catalytic domain of BjCHI1. |
| List of Research Outputs |
| Chye M.L., Zhao K.J., He Z.M., Fung K.L. and Ramalingam S., Transgenic potato protected from Rhizoctonia solani fungal infection. Made in Hong Kong Conference and Exhibition, Hong Kong Institution of Science 11th Annual Conference 2003. Science Park, Shatin, 8th November, 2003. Booth 34. |
| Li H. and Chye M.L., Arabidopsis acyl-CoA binding protein ACBP2 interacts with an ethylene-responsive element binding protein AtEBP via its ankyrin repeats, Plant Molecular Biology. 2004, 54: 233-243. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, 16th International Symposium on Plant Lipids, Budapest, Hungary, 1-4th June, 2004. Abstract 3-4: p. 94. |
| Nagegowda D.A., Bach T. and Chye M.L., Characterization of Brassica juncea HMG-CoA synthase 1, Plant Biology 2003 (American Society of Plant Biologists), Honolulu, Hawaii, USA, July 25-30, 2003. Abstract 1406. |
| Sin S.F. and Chye M.L., Expression of proteinase inhibitor II proteins during floral development, 10th Congress of the Federation of Asian and Oceanic Biochemists and Molecular Biologists, Bangalore, India, December 7-11, 2003. Session 8 Mechanisms of plant development: p. 65. |
| Ubhayasekara W., Tang C.M., Bergfors T., Berglund G., Chye M.L. and Mowbray S.L., Glycoside hydrolase family 19 Brassica juncea chitinase crystal structures, Eight Annual Conference of the Swedish Structural Biology Network, Tallberg, Sweden, 11-14th June, 2004. Poster 10. |
| Xu Z., Teng W.L. and Chye M.L., Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a, Planta. 2004, 218: 623-629. |
| Researcher : Collado LS |
| List of Research Outputs |
| Collado L.S. and Corke H., Noodles: starch, In: "Encyclopedia of Grain Science", (C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 2: Pages 293-304. |
| Tam L.M., Corke H., Tan W.T., Li J. and Collado L.S., Production of bihon-type noodles from maize starch differing in amylose content, Cereal Chemistry. 2004, 81: 475-480. |
| Researcher : Corke H |
| Project Title: | A co-operative venture in food science education with the American Association of Cereal Chemists |
| Investigator(s): | Dr. Corke H. |
| Department: | Botany |
| Source(s) of Funding: | Leung Kau Kui Research and Teaching Endowment Fund |
| Start Date: | 02/1995 |
| Abstract: |
| Initiate a programme of international short courses in food sciece in Hong Kong; initiate an English to Chinese food science textbook translation programme. |
| Project Title: | The diversity of cereal grain products in Hong Kong and southern China |
| Investigator(s): | Dr. Corke H. |
| Department: | Botany |
| Source(s) of Funding: | Woo Ting Sang Agricultural Development Research Fund |
| Start Date: | 01/1996 |
| Abstract: |
| The cereal industry in this region is incredibly diverse. There is a vast expansion of diversity fueled by increasing consumer affluence in China, and by the need for convenience products. What attributes of the cereal grain raw materials are most relaated to quality of these products? What additives and modified starches will be in most demand in future? Without basic scientific analysis of products on the market, these questions cannot be answered. |
| List of Research Outputs |
| Bao J., Sun M., Zhu L. and Corke H., Analysis of quantitative trait loci for some starch properties of rice (Oryza sativa L.): thermal properties, gel texture and swelling volume, Journal of Cereal Science. 2004, 39: 379-385. |
| Bao J., Sun M. and Corke H., Genetic diversity of starch physicochemical properties of waxy rice (Oryza sativa L.), Journal of the Science of Food and Agriculture. 2004, 84: 1299-1306. |
| Bao J. and Corke H., Rice: genetics, In: "Encyclopedia of Grain Science",(C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 3: Pages 48-61. |
| Beta T. and Corke H., Effect of ferulic acid and catechin on starch pasting properties, Cereal Chemistry. 2004, 81: 418-422. |
| Cai Y., Luo Q., Sun M. and Corke H., Antioxidant activity and phenolic compounds of 112 traditional Chinese medicinal plants associated with anticancer, Life Sciences. 2004, 74: 2157-2184. |
| Cai Y., Corke H. and Li W., Buckwheat, In: "Encyclopedia of Grain Science", (C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 1: Pages 120-128. |
| Cai Y., Corke H. and Wu H.X., Amaranthus, In: "Encyclopedia of Grain Science", (C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 1: Pages 1-10. |
| Collado L.S. and Corke H., Noodles: starch, In: "Encyclopedia of Grain Science", (C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 2: Pages 293-304. |
| Corke H. and Cai Y., Grain production and consumption - Asia, In:"Encyclopedia of Grain Science",(C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 2: Pages 77-86. |
| Corke H., Grain: morphology of internal structure, In: "Encyclopedia of Grain Science", (C.W Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 2: Pages 30-38. |
| Dissanayake Mudiyans A.G. and Corke H., Starch: analysis of quality, In: "Encyclopedia of Grain Science",(C.W. Wrigley, H. Corke, and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 3: Pages 202-212. |
| Huang Y., Corke H. and Li J.S., Coix, In: C.W. Wrigley, H. Corke and C.E. Walker eds., Encyclopedia of Grain Science. Oxford, Elsevier, 2004, Vol. 1: Pages 317-322. |
| Tam L.M., Corke H., Tan W.T., Li J. and Collado L.S., Production of bihon-type noodles from maize starch differing in amylose content, Cereal Chemistry. 2004, 81: 475-480. |
| Wrigley C.W., Corke H. and Walker C.E., Encyclopedia of Grain Science. Oxford, Elsevier, 2004, Volume 1: 412 pages. |
| Wrigley C.W., Corke H. and Walker C.E., Encyclopedia of Grain Science. Oxford, Elsevier, 2004, Volume 2: 500 pages. |
| Wrigley C.W., Corke H. and Walker C.E., Encyclopedia of Grain Science . Oxford, Elsevier, 2004, Volume 3: 512 pages. |
| Researcher : Dissanayake Mudiyans AG |
| List of Research Outputs |
| Dissanayake Mudiyans A.G. and Corke H., Starch: analysis of quality, In: "Encyclopedia of Grain Science",(C.W. Wrigley, H. Corke, and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 3: Pages 202-212. |
| Researcher : Fan KW |
| List of Research Outputs |
| Jiang Y., Fan K.W., Wong R.T.Y. and Chen S.F., Fatty acid composition and squalene content of the marine microalga Schizochytrium mangrovei, Journal of Agricultural and Food Chemistry. 2004, 52: 1196-1200. |
| Researcher : Fung KL |
| List of Research Outputs |
| Chye M.L., Zhao K.J., He Z.M., Fung K.L. and Ramalingam S., Transgenic potato protected from Rhizoctonia solani fungal infection. Made in Hong Kong Conference and Exhibition, Hong Kong Institution of Science 11th Annual Conference 2003. Science Park, Shatin, 8th November, 2003. Booth 34. |
| Researcher : Huang J |
| List of Research Outputs |
| Huang J. and Chen S.F., Differential expression of three b-carotene ketolase genes in a unicellular chlorophyte. Abstracts and Program, 5th Asia-Pacific Conference on Algal Biotechnology, 18-21 October 2003. Qingdao, China, pp. 132. |
| Lai J., Jiang Y., He X.W., Huang J. and Chen S.F., Separation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheres, Journal of Chromatography B. 2004, 804(1): 25-30. |
| Researcher : Ip PF |
| List of Research Outputs |
| Ip P.F. and Chen S.F., Enhanced production of a high-value carotenoid astaxanthin by the green microalga Chlorella zofingiensis with ROS and RNS, Conference Handbook, Great Science! Great Food! 20-23 June 2004. Hamilton, New Zealand, p. 41. |
| Researcher : Lai J |
| List of Research Outputs |
| Lai J., Jiang Y., He X.W., Huang J. and Chen S.F., Separation and determination of astaxanthin from microalgal and yeast samples by molecularly imprinted microspheres, Journal of Chromatography B. 2004, 804(1): 25-30. |
| Lai J., He X.W. and Chen S.F., Separation and determination of the antitumor drug piritrexim by molecularly imprinted microspheres in high performance liquid chromatography, Analytical and Bioanalytical Chemistry. 2003, 377(1): 208-213. |
| Researcher : Lam E |
| Project Title: | Production of dsRNA in transgenic plants for gene inactivation in mammals: novel vehicle for gene therapy and functional genomics |
| Investigator(s): | Prof. Lam E. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 10/2002 |
| Completion Date: | 09/2003 |
| Abstract: |
| In this proposal, we will construct vectors to produce dsRNA signals in transgenic plants in order to test if mammalian genes in mice and rats can be suppressed by this approach. This work will help set the stage to test if transgenic plants can be used deliver dsRNA signals to suppress expression of specific target genes in mammals. If successful, this would open a novel approach for gene therapy of certain diseases as well as systemic functional studies of all mammalian genes. |
| Researcher : Law HY |
| List of Research Outputs |
| Law H.Y. and Ma C.Y., Study of vicilin from Dolichos lablab by Fourier-transform infrared spectroscopy (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Researcher : Li H (Li Hongye) |
| List of Research Outputs |
| Li H. and Chye M.L., Arabidopsis acyl-CoA binding protein ACBP2 interacts with an ethylene-responsive element binding protein AtEBP via its ankyrin repeats, Plant Molecular Biology. 2004, 54: 233-243. |
| Researcher : Li H (Li Huabin) |
| List of Research Outputs |
| Li H., Jiang Y. and Chen S.F., Separation and purification of bioactive components from the Chinese medicinal plant Scutellaria baicalensis, Eighth International People-Plant Symposium 4-6 June, Awaji Island. Hyogo, Japan, 2004, Programme and Abstracts: pp. 39-40. |
| Researcher : Li W |
| List of Research Outputs |
| Cai Y., Corke H. and Li W., Buckwheat, In: "Encyclopedia of Grain Science", (C.W. Wrigley, H. Corke and C.E. Walker, eds.). Oxford, Elsevier, 2004, Vol. 1: Pages 120-128. |
| Researcher : Liu X |
| List of Research Outputs |
| Liu X. and Chen S.F., Cell differentiation and colony alteration of Nostoc flagelliforme, an edible terrestrial cyanobacterium, in different liquid suspension cultures, Folia Microbiologica. 2003, 48(5): 619-626. |
| Liu X., Chen S.F. and Jiang Y., Differentiation of Nostoc flagelliforme and its neighboring species using fatty acid profiling as a chemotaxonomic tool, Current Microbiology. 2003, 47(6): 467-474. |
| Researcher : Lo CSC |
| Project Title: | Functional genomics study of flavonid pathways in rice |
| Investigator(s): | Dr. Lo C.S.C. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 10/2002 |
| Completion Date: | 09/2003 |
| Abstract: |
| To study the flavonoid metabolism in rice. Extensive genomics resources for rice, including EST databases, genomic sequence data, physical and genetic maps, genomics libraries on high-density filters, and cDNA microarrays, are now available in the public domain. |
| Project Title: | Identification of proteins targeted for degradation in Arabidopsis thaliana during disease resistance responses |
| Investigator(s): | Dr. Lo C.S.C., Prof. Lam E. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 10/2003 |
| Abstract: |
| To employ two complementary technologies, namely exon-trapping and proteomics, to identify potential proteins that are targeted for degradation during defense responses to P. parasitica in Arabidopsis; to identify a list of candidate proteins for future characterizations. |
| Project Title: | Identification of proteins targeted for degradation in Arabidopsis thaliana during disease resistance responses |
| Investigator(s): | Dr. Lo C.S.C., Prof. Lam E. |
| Department: | Botany |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 10/2003 |
| Abstract: |
| To employ two complementary technologies, namely exon-trapping and proteomics, to identify potential proteins that are targeted for degradation during defense responses to P. parasitica in Arabidopsis; to identify a list of candidate proteins for future characterizations. |
| Project Title: | Functional characterization of the chalcone synthase gene family in Sorghum bicolor |
| Investigator(s): | Dr. Lo C.S.C. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 02/2004 |
| Abstract: |
| To develop research tools and generate resources that will be useful and critical for complete characterization of the biological and biochemical functions of the individual CHS genes in sorghum. |
| List of Research Outputs |
| Lo C.S.C., Adachi K., Shuster J.R., Hamer J.E. and Hamer L., The bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi, Nucleic Acids Research. 2003, 31(16): 4822-4827. |
| Researcher : Ma CY |
| Project Title: | Structure-function study of rice seed proteins |
| Investigator(s): | Prof. Ma C.Y. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2001 |
| Abstract: |
| To isolate and purify the four major Osborne protein fractions from rice seeds, and to conduct a detailed structural analysis of the proteins and their major subfractions; to study the physicochemical and thermal denaturation, aggregation and gelation properties of the rice storage proteins. |
| Project Title: | Application of raman spectroscopy for analysis of chemically modified food proteins |
| Investigator(s): | Prof. Ma C.Y. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2002 |
| Abstract: |
| Food proteins have been chemically modified by various methods to improve their functional properties to enhance their utilization as food ingredients in formulated foods. |
| Project Title: | Analysis of chemically modified non-starch polysaccharides by Raman spectroscopy |
| Investigator(s): | Prof. Ma C.Y. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2003 |
| Abstract: |
| To apply the technique of Raman spectroscopy for determining degrees of chemical modification in proteins, and extend the technique to study modified non-starch polysaccharides. |
| List of Research Outputs |
| Choi S.M., Zhao Y. and Ma C.Y., Raman spectroscopic study of buckwheat globulin basic subunit (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Choi S.M., Zhao Y. and Ma C.Y., Study of heat-induced aggregation of buckwheat globulin basic subunit (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Law H.Y. and Ma C.Y., Study of vicilin from Dolichos lablab by Fourier-transform infrared spectroscopy (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Ma C.Y. and Weerakoon S.K., Study of rice globulin conformation by Raman spectroscopy (Abstract), Second International Conference on Biomedical Spectroscopy. London, U.K., 2003. |
| Yu Z., Ma C.Y., Yuen S.N. and Phillips D.L., Raman Spectroscopic Determination of Extent of O-esterification in Acetylated Soy Protein Isolates, Food Chemistry. 2003, 87: 477-481. |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Study of Succinylated Food Proteins by Raman Spectroscopy , Journal of Agricultural and Food Chemistry. 2004, 52: 1815-1823. |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Study of acetylated food proteins by Raman spectroscopy, Journal of Food Science. 2004, 69: 206-213. |
| Zhao Y., Mine Y. and Ma C.Y., Study of thermal aggregation of oat globulin by laser light scattering, Journal of Agricultural and Food Chemistry. 2004, 52: 3089-3096. |
| Researcher : Mishra G |
| List of Research Outputs |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, 16th International Symposium on Plant Lipids, Budapest, Hungary, 1-4th June, 2004. Abstract 3-4: p. 94. |
| Researcher : Nagegowda DA |
| List of Research Outputs |
| Nagegowda D.A., Bach T. and Chye M.L., Characterization of Brassica juncea HMG-CoA synthase 1, Plant Biology 2003 (American Society of Plant Biologists), Honolulu, Hawaii, USA, July 25-30, 2003. Abstract 1406. |
| Researcher : Ramalingam S |
| List of Research Outputs |
| Chye M.L., Zhao K.J., He Z.M., Fung K.L. and Ramalingam S., Transgenic potato protected from Rhizoctonia solani fungal infection. Made in Hong Kong Conference and Exhibition, Hong Kong Institution of Science 11th Annual Conference 2003. Science Park, Shatin, 8th November, 2003. Booth 34. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, 16th International Symposium on Plant Lipids, Budapest, Hungary, 1-4th June, 2004. Abstract 3-4: p. 94. |
| Researcher : Sin SF |
| List of Research Outputs |
| Sin S.F. and Chye M.L., Expression of proteinase inhibitor II proteins during floral development, 10th Congress of the Federation of Asian and Oceanic Biochemists and Molecular Biologists, Bangalore, India, December 7-11, 2003. Session 8 Mechanisms of plant development: p. 65. |
| Researcher : Tang CM |
| List of Research Outputs |
| Ubhayasekara W., Tang C.M., Bergfors T., Berglund G., Chye M.L. and Mowbray S.L., Glycoside hydrolase family 19 Brassica juncea chitinase crystal structures, Eight Annual Conference of the Swedish Structural Biology Network, Tallberg, Sweden, 11-14th June, 2004. Poster 10. |
| Researcher : Teng WL |
| List of Research Outputs |
| Xu Z., Teng W.L. and Chye M.L., Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a, Planta. 2004, 218: 623-629. |
| Researcher : Tsang JSH |
| Project Title: | Analysis of the regulation of dehalogenase IVa gene of Burkholderia cepacia MBA4 |
| Investigator(s): | Dr. Tsang J.S.H. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2000 |
| Abstract: |
| To investigate the regulation of hdlIVa. Molecular biology techniques will be used to unravel the regulatory elements of the gene and the regulatory factor(s) identified. Understanding how the hdlIVa gene is regulated could provide information for the development of a biosensor detection system for halogenated pollutants. |
| Project Title: | Modernization of the teaching environment for molecular biology related courses |
| Investigator(s): | Dr. Tsang J.S.H. |
| Department: | Botany |
| Source(s) of Funding: | Run Run Shaw Research and Teaching Endowment Fund |
| Start Date: | 01/2002 |
| Abstract: |
| To modernize the provision of apparatus and equipment in oder to provide more hands-on training for undergraduate students. |
| Project Title: | Global analysis of burkholderia cepacia MBA4 growing on haloacid and alkanoic acid media |
| Investigator(s): | Dr. Tsang J.S.H. |
| Department: | Botany |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 07/2003 |
| Abstract: |
| To investigate the global response of MBA4 to haloacid. |
| Project Title: | Genomic analysis of the growth of Burkholderia cepacia MBA4 in haloacid and non-haloacid conditions |
| Investigator(s): | Dr. Tsang J.S.H. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 02/2004 |
| Abstract: |
| To understand the adaptation of the bacterium in different environment. |
| List of Research Outputs |
| Wong H.P.S., Chung Y.K.W. and Tsang J.S.H., Identification of the regulatory element of dehalogenase IVa gene of Burkholderia cepacia MBA4, In Abstracts of the XIX International Congress of Genetics. Melbourne, Australia, 2003, pp. 185. |
| Researcher : Wang L |
| List of Research Outputs |
| Yau C.P., Wang L., Yu M., Zee S.S.Y. and Yip W.K., Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions, Journal of Experimental Botany. 2004, 55(397): 547-556. |
| Zee S.S.Y., Ye X., Wang L., Yau C.P. and Yip W.K., F-actin visualization in generative and sperm cells of living pollen of rice using a GFP-mouse talin fusion protein, Acta Botanica Sinica. 2003, 45(8): 949-958. |
| Researcher : Wang SB |
| List of Research Outputs |
| Wang S.B., Hu Q., Sommerfeld M. and Chen S.F., An optimized protocol for isolation of soluble proteins from microalgae by two-dimensional gel electrophoresis analysis, Journal of Applied Phycology. 2003, 15: 485-496. |
| Wang S.B., Hu Q., Sommerfeld M. and Chen S.F., Cell wall proteomics of the green alga Haematococcus pluvialis (Chlorophyceae), Proteomics. 2004, 4: 692-708. |
| Wang S.B., Hu Q., Sommerfeld M. and Chen S.F., Proteomic analysis of molecular response to oxidative stress of the green alga Haematococcus pluvialis (chlorophyceae). Abstracts and Program, 5th Asia-Pacific Conference on Algal Biotechnology, 18-21 October 2003. Qingdao, China, pp. 137. |
| Researcher : Weerakoon SK |
| List of Research Outputs |
| Ma C.Y. and Weerakoon S.K., Study of rice globulin conformation by Raman spectroscopy (Abstract), Second International Conference on Biomedical Spectroscopy. London, U.K., 2003. |
| Researcher : Wen Z |
| List of Research Outputs |
| Wen Z. and Chen S.F., Heterotrophic production of eicosapentaenoic acid by microalgae, Biotechnology Advances. 2003, 21(4): 273-294. |
| Researcher : Wong HPS |
| List of Research Outputs |
| Wong H.P.S., Chung Y.K.W. and Tsang J.S.H., Identification of the regulatory element of dehalogenase IVa gene of Burkholderia cepacia MBA4, In Abstracts of the XIX International Congress of Genetics. Melbourne, Australia, 2003, pp. 185. |
| Researcher : Xu Z |
| List of Research Outputs |
| Xu Z., Teng W.L. and Chye M.L., Inhibition of endogenous trypsin- and chymotrypsin-like activities in transgenic lettuce expressing heterogeneous proteinase inhibitor SaPIN2a, Planta. 2004, 218: 623-629. |
| Researcher : Yau CP |
| List of Research Outputs |
| Yau C.P., Wang L., Yu M., Zee S.S.Y. and Yip W.K., Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions, Journal of Experimental Botany. 2004, 55(397): 547-556. |
| Zee S.S.Y., Ye X., Wang L., Yau C.P. and Yip W.K., F-actin visualization in generative and sperm cells of living pollen of rice using a GFP-mouse talin fusion protein, Acta Botanica Sinica. 2003, 45(8): 949-958. |
| Researcher : Ye X |
| List of Research Outputs |
| Zee S.S.Y., Ye X., Wang L., Yau C.P. and Yip W.K., F-actin visualization in generative and sperm cells of living pollen of rice using a GFP-mouse talin fusion protein, Acta Botanica Sinica. 2003, 45(8): 949-958. |
| Researcher : Yip WK |
| Project Title: | Molecular cloning and physiological studies of the ethylene biosynthesis genes in rice and in wheat |
| Investigator(s): | Dr. Yip W.K. |
| Department: | Botany |
| Source(s) of Funding: | Croucher Foundation |
| Start Date: | 12/1993 |
| Completion Date: | 12/2003 |
| Abstract: |
| The aims of the project include (i) Molecualr cloning the cDNAs of ACC synthase and ACC oxidase in rice and in wheat; (ii) to test for the physiological relevant of the resulted clones; (iii) expression of the gene products; promoter analysis of the corresponding genomic genes; (iv) analysis of the functions of the genes in vivo in transgenic plants by incorporating these genes into the plant genome. |
| Project Title: | Physiological studies of tomato plants transformed with various antisense ACC synthase genes |
| Investigator(s): | Dr. Yip W.K. |
| Department: | Botany |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2003 |
| Abstract: |
| To examine the gene silencing effects of ACS2 and ACS4 on tomato fruit ripening and development; to examine the effects of ACS1a and ACS6 on System I ethylene biosynthesis during tomato fruit development and ripening; to investigate auxin-induced ethylene physiological impacts by gene silencing of ACS3 and ACS5 in tomatoes. |
| Project Title: | Physiological studies of tomato plants transformed with various antisense ACC synthase genes |
| Investigator(s): | Dr. Yip W.K. |
| Department: | Botany |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 09/2003 |
| Abstract: |
| To examine the gene silencing effects of ACS2 and ACS4 on tomato fruit ripening and development; to examine the effects of ACS1a and ACS6 on System I ethylene biosynthesis during tomato fruit development and ripening; to investigate auxin-induced ethylene physiological impacts by gene silencing of ACS3 and ACS5 in tomatoes. |
| List of Research Outputs |
| Lim B.L. and Yip W.K., Recombinant Bacillus Phytases and uses thereof, The University of Hong Kong (patent filed). 2003. |
| Yau C.P., Wang L., Yu M., Zee S.S.Y. and Yip W.K., Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions, Journal of Experimental Botany. 2004, 55(397): 547-556. |
| Yip W.K., Wang L.J., Cheng C.W., Wu W.L., Lung S.C. and Lim B.L., The Introduction of a phytase gene from Bacillus subtilis improved the growth performance of transgenic tobacco, Biochemical and Biophysical Research Communications. 2003, 310: 1148-1154. |
| Zee S.S.Y., Ye X., Wang L., Yau C.P. and Yip W.K., F-actin visualization in generative and sperm cells of living pollen of rice using a GFP-mouse talin fusion protein, Acta Botanica Sinica. 2003, 45(8): 949-958. |
| Researcher : Yu M |
| List of Research Outputs |
| Yau C.P., Wang L., Yu M., Zee S.S.Y. and Yip W.K., Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions, Journal of Experimental Botany. 2004, 55(397): 547-556. |
| Researcher : Yuen SN |
| List of Research Outputs |
| Yu Z., Ma C.Y., Yuen S.N. and Phillips D.L., Raman Spectroscopic Determination of Extent of O-esterification in Acetylated Soy Protein Isolates, Food Chemistry. 2003, 87: 477-481. |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Study of Succinylated Food Proteins by Raman Spectroscopy , Journal of Agricultural and Food Chemistry. 2004, 52: 1815-1823. |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Study of acetylated food proteins by Raman spectroscopy, Journal of Food Science. 2004, 69: 206-213. |
| Researcher : Zee SSY |
| Project Title: | Studies on the control of flowering in Kumquat (Fortunella margarita) |
| Investigator(s): | Prof. Zee S.S.Y. |
| Department: | Botany |
| Source(s) of Funding: | Woo Ting Sang Agricultural Development Research Fund |
| Start Date: | 08/1987 |
| Abstract: |
| To investigate the effect of water stress on Kumquat flower production. |
| Project Title: | The biotechnological development and conservation of ornamental plants |
| Investigator(s): | Prof. Zee S.S.Y., Prof. Ye X.L. |
| Department: | Botany |
| Source(s) of Funding: | Croucher Foundation |
| Start Date: | 07/1992 |
| Completion Date: | 12/2003 |
| Abstract: |
| To seek funding to expand the collaboration to include cultivation of ornamental plants by modern biotechnology techniques. |
| Project Title: | Extraction and fractionation of Zanthoxylum alatum using the hypercritical CO2 technique |
| Investigator(s): | Prof. Zee S.S.Y., Dr. Yan F. |
| Department: | Botany |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2002 |
| Completion Date: | 10/2003 |
| Abstract: |
| To use a newly developed lipophilic extraction method (viz. the hypercritical CO2 technique) to exact and purify the lipophilic active-ingredients from the dried fruits of Zanthoxylum alatum Roxb. for analysis. |
| List of Research Outputs |
| Yau C.P., Wang L., Yu M., Zee S.S.Y. and Yip W.K., Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions, Journal of Experimental Botany. 2004, 55(397): 547-556. |
| Zee S.S.Y., Ye X., Wang L., Yau C.P. and Yip W.K., F-actin visualization in generative and sperm cells of living pollen of rice using a GFP-mouse talin fusion protein, Acta Botanica Sinica. 2003, 45(8): 949-958. |
| Researcher : Zhao Y |
| List of Research Outputs |
| Choi S.M., Zhao Y. and Ma C.Y., Raman spectroscopic study of buckwheat globulin basic subunit (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Choi S.M., Zhao Y. and Ma C.Y., Study of heat-induced aggregation of buckwheat globulin basic subunit (Abstract), Institute of Food Technologists Annual Meeting. (Chicago, IL), 2003. |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Study of Succinylated Food Proteins by Raman Spectroscopy , Journal of Agricultural and Food Chemistry. 2004, 52: 1815-1823. |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Study of acetylated food proteins by Raman spectroscopy, Journal of Food Science. 2004, 69: 206-213. |
| Zhao Y., Mine Y. and Ma C.Y., Study of thermal aggregation of oat globulin by laser light scattering, Journal of Agricultural and Food Chemistry. 2004, 52: 3089-3096. |