HKU-PASTEUR RESEARCH CENTRE LIMITED
| Researcher : Altmeyer RM |
| List of Research Outputs |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |
| Researcher : Bruzzone R |
| List of Research Outputs |
| Bruzzone R., IBRO World Congress of Neuroscience 2007. 2007. |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |
| Researcher : Chu SL |
| List of Research Outputs |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Researcher : Garcia J |
| List of Research Outputs |
| Garcia J., De Jong M., Hien V.H., Ha D.Q., Govorkova E. and Peiris J.S.M., Evaluation of H5 pseudoparticle for serology testing in humans and animals., X international Symposium on respiratory viral infections. 2008. |
| Garcia J., Young Investigator Award, X international Symposium on respiratory viral infections, Singapore. 2008. |
| Haselhorst T., Garcia J., Islam T., Lai J.C.C., Rose F.J., Nicholls J.M., Peiris J.S.M. and von Itzstein M., Avian influenza H5-containing virus-like particles (VLPs): host-cell receptor specificity by STD NMR spectroscopy, Angewandte Chemie (International ed. in English). 2008, 47(10): 1910-2. |
| Nicholls J.M. and Garcia J., Determination of the haemagglutinin - sialic acid receptor interaction of H5N1 and other influenza viruses at the atomic level, Research on Infectious Diseases, Pasteur Insitute, Paris. 2008. |
| Vong S., Cavailler P., Garcia J., Chu S. and Buchy P., Risk factors associated with sub clinical Avian Influenza A/H5N1 Virus Infection, Research on Infectious Diseases: A Global Challenge, Pasteur Institute. 2008. |
| Researcher : Ho JWY |
| List of Research Outputs |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Researcher : Jaume MDA |
| List of Research Outputs |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |
| Jaume M.D.A., Vaccination and Enhancement of Viral Entry, 3rd Mediterranean Immunology Course (MOROCCO). 2007. |
| Researcher : Kam YW |
| List of Research Outputs |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |
| Researcher : Kien FS |
| List of Research Outputs |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |
| Researcher : Kudelko M |
| List of Research Outputs |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Researcher : Lai JCC |
| List of Research Outputs |
| Haselhorst T., Garcia J., Islam T., Lai J.C.C., Rose F.J., Nicholls J.M., Peiris J.S.M. and von Itzstein M., Avian influenza H5-containing virus-like particles (VLPs): host-cell receptor specificity by STD NMR spectroscopy, Angewandte Chemie (International ed. in English). 2008, 47(10): 1910-2. |
| Researcher : Li PH |
| List of Research Outputs |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |
| Researcher : Nal-Rogier BTM |
| List of Research Outputs |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |
| Researcher : Peiris JSM |
| Project Title: | The application of DNA arrays to characterise the avian influenza gene pool in southern China |
| Investigator(s): | Peiris JSM, Guan Y, Shortridge KF |
| Department: | Microbiology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 12/2002 |
| Abstract: |
| This project is a multi-institutional collaborative study for developing a high throughput DNA array to allow genotyping of influenza virus isolates of avian origin from Hong Kong and applying it to characterize the influenza virus gene pool in sourthern China. Through its application, it is expected to:- 1) identify the precursor virus that donated its internal genes to generate the mutiple reassortment of H5N1 isolated in Hong Kong markets in 2001; 2) define the frequency of reassortment between viruses of different virus lineages that co-circulate in poultry in Hong Kong and correlate this to host-specificity; and 3) investigate whether avian internal gene segments are transiently introduced into the human influenza gene pool. |
| Project Title: | Influenza pandemic preparedness in Asia (Research collaboration with national Institute of Allergy and Infectious Diseases (NIAID), St. Jude Children's Research Hospital, Inc. (SJCRH) and The University of Hong Kong) |
| Investigator(s): | Peiris JSM, Guan Y |
| Department: | Microbiology |
| Source(s) of Funding: | National Institutes of Health, US Department of Health and Human Services - General Award |
| Start Date: | 04/2003 |
| Abstract: |
| To study influenza pandemic preparedness in Asia. |
| Project Title: | Virus-cell interaction and the pathogenesis of SARS |
| Investigator(s): | Peiris JSM |
| Department: | Microbiology |
| Source(s) of Funding: | VCO SARS Research Fund |
| Start Date: | 07/2003 |
| Abstract: |
| To explore what is the biological basis for the selective attraction of macrophages to the lung; to study what immunopathological consequences follow. |
| Project Title: | Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease? |
| Investigator(s): | Peiris JSM, Guan Y, Lau ASY, Poon LLM |
| Department: | Microbiology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2003 |
| Abstract: |
| To identify the viral genetic determinants responsible for the differential cytokine hyper-induction associated with H5N1/97 viruses. We have already established the role of the viral NS gene, however, a role for other viral genes has not been excluded. we hypothesize (and have preliminary evidence) that one or more other viral genes are also involved in the hyper-induction of cytokines; to determine whether the avian viral genes/gene products of H5N1/97 directly up-regulate cytokine induction or lack "immune evasion" mechanisms evolved by human influenza viruses? to determine whether the differential up-regulation of cytokines associated with H5N1/97 is also observed in human lung epithelial cells (primary bronchial epithelial cells and human lung epithelial cell lines e.g. A549 cells. |
| Project Title: | The role of influenza virus gene constellation and viral morphology on cytokine induction, pathogenesis and viral virulence |
| Investigator(s): | Peiris JSM, Nicholls JM, Guan Y, Poon LLM |
| Department: | Microbiology |
| Source(s) of Funding: | Research Fund for the Control of Infectious Diseases - Full Grants |
| Start Date: | 02/2005 |
| Abstract: |
| To correlate the cytokine induction phenotype with viral genotype and morphology. |
| Project Title: | The macrophage in the pathogenesis of Severe Acute Respiratory Syndrome Coronavirus infection |
| Investigator(s): | Peiris JSM |
| Department: | Microbiology |
| Source(s) of Funding: | Research Fund for the Control of Infectious Diseases - Full Grants |
| Start Date: | 12/2005 |
| Abstract: |
| The global gene expression profiling of SARS-CoV infected macrophages will provide the basis for a more detailed analysis of the signaling pathways involved in subsequent studies, and may provide rational and novel therapeutic options to be devised for treating SARS. |
| Project Title: | Mass Tag PCR: A novel, multiplex and potentially sensitive approach for the rapid detection of respiratory pathogens |
| Investigator(s): | Peiris JSM, Chiu SSS, Cheung CY |
| Department: | Microbiology |
| Source(s) of Funding: | Research Fund for the Control of Infectious Diseases - Full Grants |
| Start Date: | 06/2006 |
| Abstract: |
| To divise, optimize and evaluate the Mass Tag PCR system for the rapid disgnosis for simultaneous screening of a wide spectrum of causative agents for human respiratory tract infections, including the avian influenza H5N1 and H7N7, SARS-coronavirus (SARS-CoV). Thiw will significantly facilitate better prevention, treatment and control of infectious diseases. |
| Project Title: | Pathogenesis, cell signaling and virus evolution of avian influenza A (H5N1) |
| Investigator(s): | Peiris JSM, Guan Y, Ip NY, Lau ASY, Nicholls JM, Chen H, Poon LLM, Lau YL, Yuen KY |
| Department: | Microbiology |
| Source(s) of Funding: | Central Allocation Vote - Group Research Project |
| Start Date: | 06/2006 |
| Abstract: |
| To study the continued evolution of H5N1 influenza viruses in Vietnam and Indonesia, in the context of the ongoing studies in Hong Kong and mainland China; to define differences in cell-signaling pathways activated by avian influenza A H5N1 compared to convetional human influenza (H1N1 or H3N2) in primary human macrophages and epithelial cells; to compare the biological characteristics of H5N1 viruses from Indonesia and Vietnam to define a basis for possible differential pathogenicity for humans; investigate the biological significance of specific viral genetic mutations/changes in recent h5N1 viruses in primary human cell culture models |
| Project Title: | The pathogenesis of avian influenza A H5N1 in humans: macrophage and epithelial cell-virus interactions in vitro and ex-vivo |
| Investigator(s): | Peiris JSM |
| Department: | Microbiology |
| Source(s) of Funding: | The Wellcome Trust - General Award |
| Start Date: | 01/2007 |
| Abstract: |
| Specific aims are to: 1) Characterise the influenza viral replication profiles in tissue explant (ex-vivo) cultures of nasopharyngeal biopsies and lung tissue and primary cell cultures of human macrophages and respiratory (nasopharyngeal, bronchial, alveolar) epithelium. 2) Charaterise the functional receptors for H5N1 virus on macrophages and nasopharyngeal epithelium. 3) Define the consequences of viral entry via alternative pathways including the entry of non-neutralised virus antibody complexes via the Fc and complement receptors, to determine how these pathways affects subsequent viral replication and /or innate immune responses such as cytokine/chemokine secretion. |
| Project Title: | Role of toll-like receptors and retinoic acid-inducible gene 1 in the induction of cytokines in SARS-coronavirus infected human primary macrophages |
| Investigator(s): | Peiris JSM |
| Department: | Microbiology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 01/2007 |
| Abstract: |
| To investigate the role of ToII-like receptors (TLR) and retinoic acid inducible gene (RIG-1) in the induction of pro-inflammatory cytokines, chemokines and interferon stimulated genes in SARS coronavirus and other coronavirus infected primary macrophages and dendritic of human and murine origin by using a) gene knock-down (siRNA); b) gene over-expression. |
| List of Research Outputs |
| Garcia J., De Jong M., Hien V.H., Ha D.Q., Govorkova E. and Peiris J.S.M., Evaluation of H5 pseudoparticle for serology testing in humans and animals., X international Symposium on respiratory viral infections. 2008. |
| Ho J.W.Y., Hershkovitz O., Peiris J.S.M., Zilka A., Bar-Ilan A., Nal-Rogier B.T.M., Chu S.L., Kudelko M., Kam Y.W., Achdout H., Mandelboim M., Altmeyer R.M., Mandelboim O., Bruzzone R. and Porgador A., H5-type Influenza Hemagglutinin Is Functionally Recognized By The Natural Killer Activating Receptor, Nkp44, In: Editor in Chief Lynn W. Enquist (2012) Princeton University , Journal Of Virology. USA, American Society for Microbiology, 2007, 82: 2028-2032. |
| Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Daeron M., Bruzzone R., Peiris J.S.M., Altmeyer R.M. and Nal-Rogier B.T.M., SARS-CoV subunit vaccine: Mechanisms of antibody-mediated neutralisation and investigation of potential antibody-mediated enhancement (ADE), Health Research Symposium 2007 (HK Food and Health Bureau). 2007. |