Dept of Microbiology

DEPT OF MICROBIOLOGY

Researcher : Bahl J

List of Research Outputs

Al-Azemi A., Bahl J., Al-Zenki S., Al-Shayji Y., Al-Amad S., Chen H., Guan Y., Peiris J.S.M. and Smith G.J., Avian influenza A (H5N1) virus outbreaks in Kuwait, 2007, Emerging Infectious Diseases. 2008, 14: 958-961.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Dhanasekaran V., Bahl J. and Guan Y., Estimating dates of influenza A (H5N1) virus transmission, 6th Asia-Pacific Bioinformatics Conference, Kyoto, Japan. 2008.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Researcher : Cao K

List of Research Outputs

Cao K., Mao X.P., Wang D.H., Xiao N., Xu L., Yuan G.Q., Dai S.Q., Zheng B. and Qiu S.P., High Expression of PSM-E Correlated with Tumor Grade in Prostate Cancer: A New Alternatively Spliced Variant of Prostate-Specific Membrane Antigen., Prostate. 2007, 67(16): 1791-1800.

Researcher : Chan CM

List of Research Outputs

Chan K.H., Sonnenberg K., Niedrig M., Lam S.Y., Pang C.M., Chan C.M., Ma S.K., Seto W.H. and Peiris J.S.M., Use of antibody avidity assays for diagnosis of severe acte respiratory syndrome coronavirus infection, Clinical and Vaccine Immunology. 2007, 14(11): 1433-6.

Researcher : Chan CS

List of Research Outputs

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Du L., Zhao G., Lin Y., Chan C.S., He Y., Jian S., Wu C.Y., Jin D., Yuen K.Y., Zhou Y. and Zheng B., Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection, Vaccine. 2008, 26(13): 1644-1651.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., A feasibility study for detection of HIV specific cytotoxic T lymphocytes using rAAV delivery system. , 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention.. Sydney, Australia., 2007.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., A mechanism underlying susceptibility to HBV infection: HBV down-regulate transferase-1 type I interferon receptor 1 via suppressing poly(ADP-ribose), 2007 International Meeting: The Molecular Biology of Hepatitis B Viruses.. 2007.

Researcher : Chan JSY

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Researcher : Chan KCW

List of Research Outputs

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., A feasibility study for detection of HIV specific cytotoxic T lymphocytes using rAAV delivery system. , 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention.. Sydney, Australia., 2007.

Researcher : Chan KH

List of Research Outputs

Chan K.H., Peiris J.S.M., Lim W., Nicholls J.M. and Chiu S.S.S., Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children , Journal of Clinical Virology. 2008, 42(1): 65-9.

Chan K.H., Yam W.C., Pang C.M., Chan K.M., Lam S.Y., Lo K.F., Poon L.L.M. and Peiris J.S.M., Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples, Journal of Clinical Microbiology. 2008, 46(7): 2195-2199.

Chan K.H., Sonnenberg K., Niedrig M., Lam S.Y., Pang C.M., Chan C.M., Ma S.K., Seto W.H. and Peiris J.S.M., Use of antibody avidity assays for diagnosis of severe acte respiratory syndrome coronavirus infection, Clinical and Vaccine Immunology. 2007, 14(11): 1433-6.

Cheng K.Y., Cowling B.J., Ho L.M., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Validation of the quickvue rapid diagnostic test for influenza in Hong Kong community (abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 8 September 2007, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2007, Poster 12.

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Cheuk D.K.L., Tang I.W.H., Chan K.H., Woo P.C.Y., Peiris J.S.M. and Chiu S.S.S., Rhinovirus infection in hospitalized children in Hong Kong - A prospective study, The Pediatric Infectious Diseases Journal. 2007, 26: 995-1000.

Cowling B.J., Cheng K.Y., Chan K.H., Fung R.O.P., Fang J., Lam C.L.H., Ho L.M., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Evaluation of the QuickVue influenza A+B rapid diagnostic test in a community setting (abstract), 6th International Conference on Emerging Infectious Diseases, 16-19 March 2008, Atlanta, USA. Atlanta, Centers for Disease Control and Prevention, 2008, 203.

Cowling B.J., Cheng K.Y., Fung R.O.P., Fang J., Lam C.L.H., Ho L.M., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Pilot findings of a cluster randomized trial of non-pharmaceutical interventions for influenza prevention in households in Hong Kong (abstract), 6th International Conference on Emerging Infectious Diseases, 16-19 March 2008, Atlanta, USA. Atlanta, Centers for Disease Control and Prevention, 2008, 84.

Cowling B.J., Fung R.O.P., Cheng K.Y., Fang J., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Pilot findings of a randomized trial of non-pharmaceutical interventions to prevent influenza transmission in households in Hong Kong (abstract), X International Symposium on Respiratory Viral Infections, 28 February - 2 March 2008, Singapore . Singapore, The Macrae Group, 2008, I-2.

Cowling B.J., Fung R.O.P., Cheng K.Y., Fang J., Chan K.H., Seto W.H., Yung R., Chiu B., Lee P., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Preliminary findings of a randomized trial of non-pharmaceutical interventions to prevent influenza transmission in households, PLoS One. 2008, 3(5): e2101.

Fabian M.P., McDevitt J.J., Dehaan W.H., Fung R.O.P., Cowling B.J., Chan K.H., Leung G.M. and Milton D.K., Influenza virus in human exhaled breath (abstract), 6th International Conference on Emerging Infectious Diseases, 16-19 March 2008, Atlanta, USA. Atlanta, Centers for Disease Control and Prevention, 2008, 126.

Lau S.K.P., Yip C.Y., Que T.L., Lee R.A., Au-yeung R.K.H., Zhou B., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong, Journal of Infectious Diseases. 2007, 196: 986-993.

Lau S.K.P., Yip C.Y., Tsoi H.W., Lee R.A., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical features and complete genome characterization of a distinct human rhinovirus genetic cluster, probably representing a previously undetected HRV species, HRV associated with acute respiratory illness in children, Journal of Clinical Microbiology. 2007, 45(11): 3655-3664.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Xu X., Chan K.H. and Chiang A.K.S., Development of Latent and Lytic Epstein-barr Virus (EBV)-specific CD8+ Cytotoxic Lymphocyte Responses in Primary EBV Infection, 12^th Research Postgraduate Symposium, Hong Kong, 14 December 2007. 2007-12-14, 158.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Researcher : Chan KM

List of Research Outputs

Chan K.H., Yam W.C., Pang C.M., Chan K.M., Lam S.Y., Lo K.F., Poon L.L.M. and Peiris J.S.M., Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples, Journal of Clinical Microbiology. 2008, 46(7): 2195-2199.

Researcher : Chan MCW

Project Title:	Primary human lung epithelial cells as an in-vitro model to study the pathogenesis of SARS coronavirus infection
Investigator(s):	Chan MCW, Peiris JSM, Tsao GSW
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

The research objective is to define cytokine, chemokine, SARS functional receptor gene ACE2 and apoptotic related gene expression profile of human lung epithelial cells in response to infection with infectious and inactivated SARS coronavirus and purified viral proteins.

Project Title:	Replication and pathogenesis of avian influenza A (H5N1) viruses in polarized human bronchial and alveolar epithelium
Investigator(s):	Chan MCW, Tsao GSW, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	03/2007

Abstract:

To establish a well differentiated and polarized primary human respiratory epithelial cell model in vitro to study and compare the replication kinetics of avian influenza H5N1 and H1N1 viruses. This model will provide insights into the pathogenesis of H5N1 disease in the lung. The role of innate immune response mediators and molecules such as mucin, collectins and glycoprotein-340 on viral replication will be investigated. The Contribution of different avian or human influenza viral genes to the viral replication and cytokine induction will be defined using recombinant influenza viruses generated by reverse genetics.

List of Research Outputs

Chan M.C.W., Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y. and Peiris J.S.M., Polarity of influenza H5N1 virus infection in human respiratory epithelial cells, Health Research Symposium 2007.

Chan M.C.W., Tropism of influenza H5N1 virus and SARS-CoV in human respiratory tract, Cardiovascular Research Institute, University of California, San Francisco, San Francisco, CA, USA. 2008.

Chan M.C.W., Tropism of influenza H5N1 virus in human upper and lower respiratory tract, Department of Physiology and Cell Biology, Harvard School of Public Health, Harvard Medical School, Harvard University, Boston, MA, USA. 2008.

Chan W.Y., Yu C.L., Nicholls J.M., Tsao G.S.W., Peiris J.S.M. and Chan M.C.W., Innate immune response and virus replication in human nasopharyngeal epithelial cells infected with influenza A (H5N1) virus, American Society for Microbiology general meeting 2008.

Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y., Peiris J.S.M. and Chan M.C.W., The use of in vitro and ex vivo model to study the tropism and pathogenesis of emerging respiratory viruses, Health Research Symposium 2007.

Researcher : Chan WY

List of Research Outputs

Chan M.C.W., Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y. and Peiris J.S.M., Polarity of influenza H5N1 virus infection in human respiratory epithelial cells, Health Research Symposium 2007.

Chan W.Y., Yu C.L., Nicholls J.M., Tsao G.S.W., Peiris J.S.M. and Chan M.C.W., Innate immune response and virus replication in human nasopharyngeal epithelial cells infected with influenza A (H5N1) virus, American Society for Microbiology general meeting 2008.

Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y., Peiris J.S.M. and Chan M.C.W., The use of in vitro and ex vivo model to study the tropism and pathogenesis of emerging respiratory viruses, Health Research Symposium 2007.

Nicholls J.M., Chan W.Y., Russell R.J., GM A.I.R. and Peiris J.S.M., Evolving complexities of influenza virus and its receptors, Trends in Microbiology. 2008, 16(4): 149-157.

Researcher : Chan WY

List of Research Outputs

Nicholls J.M., Chan W.Y., Russell R.J., GM A.I.R. and Peiris J.S.M., Evolving complexities of influenza virus and its receptors, Trends in Microbiology. 2008, 16(4): 149-157.

Researcher : Chan YO

List of Research Outputs

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., SARS-coronavirus induced different gene expression of chemokine receptors in human monocyte-derived adult and cord blood dendritic cells, The 3^rd Congress of Asian Society for Pediatric Research, Tokyo, Japan, 6-9 October 2007. 103.

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., The role of dendritic cells in SARS coronavirus infection, Health Symposium 2007, 29th September, 2007, Hong Kong. 2007.

Researcher : Chen D

List of Research Outputs

Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., A mechanism underlying susceptibility to HBV infection: HBV down-regulate transferase-1 type I interferon receptor 1 via suppressing poly(ADP-ribose), 2007 International Meeting: The Molecular Biology of Hepatitis B Viruses.. 2007.

Researcher : Chen H

Project Title:	Modification of cells for isolation and characterization of SARS CoV from animal sources
Investigator(s):	Chen H
Department:	Microbiology
Source(s) of Funding:	Seed Funding for New Staff
Start Date:	07/2004

Abstract:

To provide: (1) a panel of cell lines (pools) for the isolation of SARS CoV from animal sources; (2) information on molecular mechanism of interspecies transmission of SARS CoV; (3) clues for the further molecular virology studies on this virus.

Project Title:	Biological function/relevance for the 29 nucleotides insertion in the genome of animal SARS like CoV
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

This study will serve as a pilot investigating the potential effect of insertion/deletion on expression of viral proteins down stream of this region and provide clues for further investigation into the molecular mechanism for SARS coronavirus interspecies transmission.

Project Title:	Identification of the possible animal reservoirs for SARS-like coronavirus in southern China
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

Even though the SARS epidemic had been brought under control, the lack of understanding of the origin of SCoV make it impossible to determine wheter this disease might come back in future. The detection of SCoV-like virus in wild animal species in Guangdone raises the necessity of furhter understanding the epidemiology and ecology of SCoV or SCoV-like viruses. The objectives of this proposal 1) to identify the animal reservoirs of SCoV-like viruses; 2) to identify the susceptible species that could facilitate the SARS outbreak. 3) to establish a system for the detection and isolation of sCoV-like viruses. The findings generated by this project are of critical importance for scientific and public health points of view, and may provide clues how to prevent the re-emergence of SARS in humans.

Project Title:	Molecular basis of genesis and determinants for pathogenicity of avian influenza H5N1 virus genotype Z
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2006

Abstract:

To extend our current genetic and surveillance studies to investigate the molecular basis for the genesis of the dominant genotype Z and identify molecular determinants for the pathogenesis of H5N1 viruses, especially the potential for adaptation of H5N1 in chickens and mammals; to evaluate the replication efficiency (rate) of different H5N1 genotypes in mammalian cells using tissue culture; to analyze tissue transmission and pathogenesis of H5N1 isolates identified from 2000 to 2004 in Southern China in chickens and mice in a BL-3 facility; to investigate the relationship between pathogenicity and molecular differences using bioinformatics; to identify other possible molecular determinants of H5N1 virus pathogenesis in chicken and mice in addition to the recognized HA basic amino connecting peptide using reverse genetics techniques.

Project Title:	Antiviral drug resistance in H5N1 virus
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2007

Abstract:

To investigate the geographical distribution of resistance mutations to amantadine and rimantadine in H5N1 isolates from different regions, and to explore if low frequency naturally occuring mutations associated with resistance to oseltamivir are present in H5N1 isolates and if they may contribute to the emergence of oseltamivir resistant strains following exposure to this drug.

Project Title:	H5N1 virus heterogeneity and mechanisms for adaptation to new hosts
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2007

Abstract:

To investigate the distribution of L26I and S31N M2 mutants and H274Y NA mutants among H5N1 isolates from different geographical locations isolated at different points in time; to compare the growth and transmissibility phenotypes of mutants with wild type virus in different hosts, particularly chickens and mice; to study the growth advantage/disadvantage of M2 and NA H5N1 virus mutants in their new hosts, which will provide information on whether such mutants have the potential to gradually increase their presence and/or outgrow wild type virus to become the dominant population; to study the biological fitness of viruses carrying M2 or NA mutations in mammals and explore the pandemic potential of H5N1 virus.

List of Research Outputs

Al-Azemi A., Bahl J., Al-Zenki S., Al-Shayji Y., Al-Amad S., Chen H., Guan Y., Peiris J.S.M. and Smith G.J., Avian influenza A (H5N1) virus outbreaks in Kuwait, 2007, Emerging Infectious Diseases. 2008, 14: 958-961.

Campitelli L., Di Martino A., Spagnolo D., Smith G.J., Di Trani L., Facchini M., De Marco M.A., Foni E., Chiapponi C., Martin A.M., Chen H., Guan Y., Delogu M. and Donatelli I., Molecular analysis of avian H7 influenza viruses circulating in Eurasia in 1999–2005: detection of multiple reassortant virus genotypes, Journal of General Virology. 2008, 89: 48-59.

Chen X., Smith G.J., Zhou B., Qui C., Wu W.L., Li Y., Lu P., Duan L., Liu S., Yuan J., Yang G., Wang H., Cheng J., Jiang H., Peiris J.S.M., Chen H., Yuen K.Y., Zhong N.S. and Guan Y., Avian Influenza A (H5N1) Infection in a Patient in China, 2006, Influenza and Other Respiratory Viruses. 2008, 1: 207-213.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Cheung C.L., Fan X., Smith G.J., Zhang J., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Chu K.W., Peiris J.S.M., Chen H., Guan Y. and Poon L.L.M., Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats. , J Gen Virol. 2008, 89: 1282-7.

Dhanasekaran V., Zhang J., Smith G.J., Chen H. and Guan Y., Ecology and evolution of coronaviruses, The XIth International Nidoviral Symposium St Catherine's College, Oxford, UK, 2008.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Ducatez M.F., Chen H., Guan Y. and Muller C.P., Molecular epidemiology of chicken anemia virus (CAV) in southeastern Chinese live birds markets., Avian Diseases. 2008, 52(1): 68-73.

Guan Y., Chen H., Li K.S., Riley S., Leung G.M., Webster R., Peiris J.S.M. and Yuen K.Y., A model to control the epidemic of H5N1 influenza at the source., BMC Infectious Diseases. 2007, 13;7: 132.

Liu N., Song W., Wang P., Lee K., Chan W., Chen H. and Cai Z., Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells., Proteomics. 2008, 8: 1851-1858.

Nicholls J.M., Bourne A.J., Chen H., Guan Y. and Peiris J.S.M., Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses, Respiratory Research. 2007, 8: 73.

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Fan X., Li K., Chen H., Peiris J.S.M. and Guan Y., Ecology and evolution of influenza A (H5N1) virus in Asia: Evidence from systematic influenza surveillance, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Xu K., Zhang J., Duan L., Smith G.J., Peiris J.S.M., Chen H. and Guan Y., Evolution of H9N2 influenza A viruses in quail from southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Zhang J., Dhanasekaran V., Liu W., Dong B., Zhu H., Li L., Smith G.J., Chen H. and Guan Y., Interspecies transmission of coronaviruses in wild animals, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Researcher : Chen JHK

List of Research Outputs

Chen J.H.K., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., Molecular epidemiology and divergence of HIV type 1 protease codon 35 inserted strains among treatment-naive patients in Hong Kong, In: Chen J.H.K., Wong K.H., Chan K.C., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., AIDS Research and Human Retroviruses. 2008, 24: 537-542.

Researcher : Cheng VCC

List of Research Outputs

Au W.Y., Lie A.K.W., Cheng V.C.C., Cheng L.C., Wang E.P. and Wong C.F., Successful Lung Transplantation for Post-BMT Bronchiolitis Obliterans and Lipoid Pneumonia Associated with Atypical Mycobacterium and Aspergillosis Infection , The Journal of Heart and Lung Transplantation. 2007, 26(8): 870-872.

Chen J.H.K., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., Molecular epidemiology and divergence of HIV type 1 protease codon 35 inserted strains among treatment-naive patients in Hong Kong, In: Chen J.H.K., Wong K.H., Chan K.C., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., AIDS Research and Human Retroviruses. 2008, 24: 537-542.

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Cheng V.C.C., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection, Clinical Microbiology Reviews. 2007, 20: 660-694.

Hui C.K., Cheung W.W., Leung K.W., Cheng V.C.C., Tang S.F., Li W.S., Lee N.P., Kwong Y.L., Au W.Y., Yuen K.Y., Lau G. and Liang R.H.S., Outcome and immune reconstitution of HBV-specific immunity in patients with reactivation of occult HBV infection after alemtuzumab-containing chemotherapy regimen, Hepatology. EPub, 2008.

Researcher : Cheung CL

List of Research Outputs

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Cheung C.L., Fan X., Smith G.J., Zhang J., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Researcher : Cheung CL

List of Research Outputs

Researcher : Cheung CY

Project Title:	Determination of cellular receptors involved in differentially high pro-inflammatory induction by influenza A subtype H5N1 viruses in primary human monocyte-derived macrophages
Investigator(s):	Cheung CY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2006

Abstract:

The pandemic threat posted by avian influenza A H5N1 remains the major infectious disease threat currently confronting humankind. This virus is entrenched in poultry across a wide geographical distribution across Asia and continues to cause human disease. Although transmission to humans is inefficient at present, human disease tends to be severe with mortality rates reportedly in excess of 30%. Dissemination of the virus beyond the respiratory tract probably accounts for some of this unusual disease severity with recent H5N1 viruses. In addition, our previous studies suggest that, in contrast to conventional human influenza virus subtypes H1N1 or H3N2, the H5N1 viruses associated with human disease selectively hyper-induce pro-inflammatory cytokines from primary human macrophages (1). These include TNF alpha, IP-10, MIP1-alpha and RANTES. There is evidence that human patients with H5N1 disease have elevated levels of cytokines in their serum (2). H5N1 viruses have continued to evolve and reassort (3) and not all H5N1 virus genotypes are equally efficient at hyper-inducing the pro-inflammatory cytokines in vitro, the virus genotypes causing human disease in 1997, 2003 (see ref 4) and 2004 (unpublished data) being the most potent in this regard. The macrophages are a key sentinel cell of the immune system and an important source of pro-inflammatory cytokines, the respiratory epithelial cells are the key primary target of the influenza virus. We have therefore carried out preliminary studies on primary human bronchial and alveolar epithelial cells and find that the H5N1 virus genotypes associated with human disease hyper-induce a range of chemokines and cytokines including IP-10 and RANTES (Chan et al – in preparation). In contrast to macrophages, TNF alpha is not induced in these latter cells. Interestingly, both human (eg subtype H1N1) and avian (e.g. H5N1) viruses replicate equally efficiently in primary human respiratory epithelial cells and macrophages in vitro, a surprising observation given the supposedly different receptor preferences of avian and human viruses. The interaction and collaboration between toll-like receptors and other innate immune receptors is being increasingly recognized and the H5N1 – macrophage system would provide an excellent model in which to investigate these phenomena (5). The objectives of the present study are to define the mechanisms underlying this differential hyper-induction of cytokines by H5N1 viruses from primary human macrophages in vitro. Specifically we aim to compare human and avian viruses that are high and low cytokine inducers in vitro, in order to define viral receptors associated with innate immune sensing and virus entry in macrophages. References: 1) Cheung CY, Poon LL, Lau AS, Luk W, Lau YL, Shortridge KF, Gordon S, Guan Y, Peiris JS. Induction of proinflammatory cytokines in human macrophages by influenza A (H5N1) viruses: a mechanism for the unusual severity of human disease? Lancet. 2002 Dec 7;360(9348):1831-7. 2) Peiris JS, Yu WC, Leung CW, Cheung CY, Ng WF, Nicholls JM, Ng TK, Chan KH, Lai ST, Lim WL, Yuen KY, Guan Y. Re-emergence of fatal human influenza A subtype H5N1 disease. Lancet. 2004 Feb 21;363(9409):617-9. 3) Li KS, Guan Y, Wang J, Smith GJ, Xu KM, Duan L, Rahardjo AP, Puthavathana P, Buranathai C, Nguyen TD, Estoepangestie AT, Chaisingh A, Auewarakul P, Long HT, Hanh NT, Webby RJ, Poon LL, Chen H, Shortridge KF, Yuen KY, Webster RG, Peiris JS. Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia. Nature. 2004 Jul 8;430(6996):209-13. 4) Guan Y, Poon LL, Cheung CY, Ellis TM, Lim W, Lipatov AS, Chan KH, Sturm-Ramirez KM, Cheung CL, Leung YH, Yuen KY, Webster RG, Peiris JS. H5N1 influenza: a protean pandemic threat. Proc Natl Acad Sci U S A. 2004 May 25;101(21):8156-61. 5) Mukhopadhyay S, Herre J, Brown GD, Gordon S. The potential for Toll-like receptors to collaborate with other innate immune receptors. Immunology. 2004 Aug;112(4):521-30.

List of Research Outputs

Cheung C.Y., Host-response to influenza A H5N1, Beijing CDC, Beijing, PRC.. 2008.

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., SARS-coronavirus induced different gene expression of chemokine receptors in human monocyte-derived adult and cord blood dendritic cells, The 3^rd Congress of Asian Society for Pediatric Research, Tokyo, Japan, 6-9 October 2007. 103.

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., The role of dendritic cells in SARS coronavirus infection, Health Symposium 2007, 29th September, 2007, Hong Kong. 2007.

Lee D.C.W., Mok K.P., Law H.Y., Tam H.M., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., Mechanisms of interferon dysregulation and virus-induced cell death in Avian-Influenza H5N1 infections, Health Research Symposium 2007: Building bridges between research, practice & policy, September 29,. 2007.

Lee M.Y., Cheung C.Y., Nicholls J.M. and Peiris J.S.M., Induction of COX-2 mediated proinflammatory cascade during H5N1 infection, Health Research Symposium 2007.

Mao H., Zhou J., Tu W., Law H.K.W., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Differential expressions of cytokines and chemokines in macrophages infected with 483/97 and 1203/04 H5N1 viruses, The 3^rd Congress of Asian Society for Pediatric Research, Tokyo, Japan, 6-9 October 2007. 104.

Mao H., Zhou J., Tu W., Law H.K.W., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Differential proinflammatory responses in macrophages infected with 483/97 and 1203/04 H5N1 viruses, 12nd Research Postgraduate Symposium, LKS Faculty of Medicine, The University of Hong Kong, 12-14 December, 2007. 114.

Mao H., Tu W., Qin G., Chan P.L., Law H.K.W., Zheng J., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Enhanced activation and cytotoxicity of natural killer cells by influenza H5N1 virus-infected macrophages, Hong Kong Society for Immunology 2008 Annual General Meeting and Scientific Meeting, 19th April, 2008, HKSAR. 2008, 38.

Nicholls J.M., Chan M.C., Chan W.Y., Wong H.K., Cheung C.Y., Kwong D.L.W., Wong M.P., Chui W.H., Poon L.L., Tsao S.W., Guan Y. and Peiris J.S.M., Jropism of avian influenza A (H5N1) in the upper and lower respiratory tract, Nat Med. 2007, 13: 147-9.

Padhan K., Tanwar C., Hussain A., Hui P.Y., Lee M.Y., Cheung C.Y., Peiris J.S.M. and Jameel S., Severe Acute Respiratory Syndrome Coronavirus Orf3a Protein Interacts With Caveolin, Journal of General Virology. 2008, 88: 3067-77.

Researcher : Cheung KW

List of Research Outputs

Ng S.F., Li O.T.W., Cheung K.W., Peiris J.S.M. and Poon L.L.M., Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans., Virology Journal. 2008, 5: 2.

Researcher : Chong TK

Project Title:	Construction and characterization of delta AFMP1 delta AFMP2 deletion mutant of Aspergillus fumigatus
Investigator(s):	Chong TK, Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

In this project, we propose to study the essentiality and possible roles of AFMP1 and AFMP2 in Aspergillus fumigatus by constructing the deletion mutants, delta AFMP1, delta AFMP2 and delta AFMP1 delta AFMP2 of Aspergillus fumigatus. The neighborhoods of these genes in the genomes of A. fumigatus, A. nidulans and P. marneffei will also be explored.

List of Research Outputs

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Researcher : Chow CK

List of Research Outputs

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Researcher : Chow KH

List of Research Outputs

Chiu S.S.S., Ho P.L., Chow K.H., Yuen K.Y. and Lau Y.L., Nasopharyngeal Carriage of Antimicrobial-Resistant Streptococcus pneumoniae among Young Children Attending 79 Kindergartens and Day Care Centers in Hong Kong, Health Research Symposium 2007: Building Bridges between Research, Practice & Policy, 29 September 2007.

Ho P.L., Poon W.W.N., Loke S.L., Leung M.S.T., Chow K.H., Wong R.C.W., Yip K.S., Lai E.L.Y. and Tsang K.W.T., Community emergence of CTX-M type extended-spectrum beta-lactamases among urinary Escherichia coli from women. , Journal of Antimicrobial Chemotherarpy. 2007, 60(1): 140-144.

Ho P.L., Chiu S.S.S., Chow K.H., Mak C.K. and Lau Y.L., Pediatric hospitalization for pneumococcal diseases preventable by 7-valent pneumococcal conjugate vaccine in Hong Kong, Vaccine. 2007, 25: 6837-6841.

Researcher : Chu KW

List of Research Outputs

Chu K.W., Peiris J.S.M., Chen H., Guan Y. and Poon L.L.M., Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats. , J Gen Virol. 2008, 89: 1282-7.

Researcher : Dhanasekaran V

List of Research Outputs

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Dhanasekaran V., Zhang J., Smith G.J., Chen H. and Guan Y., Ecology and evolution of coronaviruses, The XIth International Nidoviral Symposium St Catherine's College, Oxford, UK, 2008.

Dhanasekaran V., Wang J., Smith G.J., Peiris J.S.M. and Guan Y., Evolution of Influenza A (H5N1) virus in Asia: evidence from systematic surveillance, International Conference on Emerging and Re-emerging viral diseases of the tropics and sub-tropics organized by Indian Virological Society. 2007.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Nguyen T.D., Nguyen T.V., Dhanasekaran V., Webster R.G., Guan Y., Peiris J.S.M. and Smith G.J., Multiple lineages of Influenza A (H5N1) viruses in Vietnam (2006-2007), Emerging Infectious Diseases. 2008, 14: 632-636.

Smith G.J., Dhanasekaran V., Bahl J. and Guan Y., Estimating dates of influenza A (H5N1) virus transmission, 6th Asia-Pacific Bioinformatics Conference, Kyoto, Japan. 2008.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Zhang J., Dhanasekaran V., Liu W., Dong B., Zhu H., Li L., Smith G.J., Chen H. and Guan Y., Interspecies transmission of coronaviruses in wild animals, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Researcher : Du L

List of Research Outputs

Du L., Kao R.Y.T., Zhou Y., He Y., Zhao G., Wong K.L., Jiang S., Yuen K.Y., Jin D. and Zheng B., Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity., Biochem. Biophys. Res. Commun.. 2007, 359(1): 174-179.

Du L., Jian S. and Zheng B., Development of subunit vaccines against severe acute respiratory symptom., Drugs of Today. 2008, 44(1): 63-73.

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Du L., Zhao G., Lin Y., Chan C.S., He Y., Jian S., Wu C.Y., Jin D., Yuen K.Y., Zhou Y. and Zheng B., Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection, Vaccine. 2008, 26(13): 1644-1651.

Researcher : Duan L

List of Research Outputs

Chen X., Smith G.J., Zhou B., Qui C., Wu W.L., Li Y., Lu P., Duan L., Liu S., Yuan J., Yang G., Wang H., Cheng J., Jiang H., Peiris J.S.M., Chen H., Yuen K.Y., Zhong N.S. and Guan Y., Avian Influenza A (H5N1) Infection in a Patient in China, 2006, Influenza and Other Respiratory Viruses. 2008, 1: 207-213.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Xu K., Zhang J., Duan L., Smith G.J., Peiris J.S.M., Chen H. and Guan Y., Evolution of H9N2 influenza A viruses in quail from southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Researcher : Fan X

List of Research Outputs

Cheung C.L., Fan X., Smith G.J., Zhang J., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Fan X., Li K., Chen H., Peiris J.S.M. and Guan Y., Ecology and evolution of influenza A (H5N1) virus in Asia: Evidence from systematic influenza surveillance, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Researcher : Fu CT

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Researcher : Gao Y

List of Research Outputs

Li H.Y., Chau D.H.W., Gao Y., Ng S.M. and Lin M.C., Genome-wide Expression Profiling Reveals the Multiple Signaling Pathways Modulated by rAdv-hTERTC27 Treatment in Glioblastoma Multiforme, International Conference on Bioinformatics 2007; 27-30 August 2007; Hong Kong.. 2007.

Shen Z., Yang Z., Gao Y., Li J.C., Chen H.X., Liu C.C., Poon R.T.P., Fan S.T., Luk J.M.C., Sze K.H., Li T.P., Gan R.B., He M.L., Kung H.F. and Lin M.C., The Kringle 1 Domain of Hepatocyte Growth Factor Has Antiangiogenic and Antitumor Cell Effects on Hepatocellular Carcinoma, Cancer Research. 2008, 68: 404-414.

Researcher : Gao Y

List of Research Outputs

Researcher : Guan Y

Project Title:	Vaccines for the control of H5N1 and other avian influenza viruses
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	The University of Hong Kong Foundation Seed Grant
Start Date:	04/2002

Abstract:

To study vaccines for the control of H5N1 and other avian influenza viruses.

Project Title:	Genetic determinants of influenza virus for inter-species transmission: studies using a reverse genetic approach
Investigator(s):	Guan Y, Peiris JSM, Poon LLM, Shortridge KF
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	08/2002

Abstract:

Avian influenza viruses can directly impact on human and animal health and thus cause economic loss. The study aims to establishing a reverse genetic system and to determine the biological significance of the deletion of NA stalk and the NS genes, particularly in relation to the host-restriction and pathogenesis. Results can enhance understanding of the machanism of inter-species transmission of influenza virus.

Project Title:	Searching for the precursor of SARS-Cov in Southern China
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To find source of SARS-Cov, and how this virus emerged in humans.

Project Title:	Genomic characterization, population ecology and genetics of H9N2 influenza viruses in southern China
Investigator(s):	Guan Y, Smith GJ
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	10/2006

Abstract:

To characterise the number of novel reassortnat H9N2 influenza virus genotypes circulating a poultry in southern China; to understand the ecology of H9N2 viruses in southern China and how this contributes to the generation of those novel genotypes; to determine the inter-relations of h5N1 and H9 N2 viruses that are co-circulating in poultry in southern China.

Project Title:	The role of migratory birds in the transmission of H5N1 Influenza virus in eastern Asia
Investigator(s):	Guan Y, Chen H, Smith GJ
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2007

Abstract:

To observe the epidemiology of influenza viruses of migratory birds in Southern China; to determine whether influenza viral gene pool in migratory birds contribute to generate novel reassortant H5N1 influenza viruses with pandemic potential; to investigate whether H5N1 influenza virus will become established in migratory bird fowls and result in further expansion of geographic distribution in Eurasian continent.

List of Research Outputs

Al-Azemi A., Bahl J., Al-Zenki S., Al-Shayji Y., Al-Amad S., Chen H., Guan Y., Peiris J.S.M. and Smith G.J., Avian influenza A (H5N1) virus outbreaks in Kuwait, 2007, Emerging Infectious Diseases. 2008, 14: 958-961.

Boon C., Sandbulte M.R., Seiler P., Webby R.J., Songserm T., Guan Y. and Webster R., Role of terrestrial wild birds in ecology of influenza A virus (H5N1) , Emerging Infectious Diseases. 2007, 13(11): 1720-1724.

Campitelli L., Di Martino A., Spagnolo D., Smith G.J., Di Trani L., Facchini M., De Marco M.A., Foni E., Chiapponi C., Martin A.M., Chen H., Guan Y., Delogu M. and Donatelli I., Molecular analysis of avian H7 influenza viruses circulating in Eurasia in 1999–2005: detection of multiple reassortant virus genotypes, Journal of General Virology. 2008, 89: 48-59.

Carrington C.V.F., Foster J.E., Zhang J., Li L., Smith G.J., Thompson N., Auguste A.J., Ramkissoon V., Adesiyun A.A. and Guan Y., Group 1 coronaviruses detected in Trinidadian bats, Keystone Global Health Symposium "Molecular Evolution as a Driving Force in Infectious Diseases”, 2008, CO, USA. 2008.

Chan M.C.W., Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y. and Peiris J.S.M., Polarity of influenza H5N1 virus infection in human respiratory epithelial cells, Health Research Symposium 2007.

Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y., Peiris J.S.M. and Chan M.C.W., The use of in vitro and ex vivo model to study the tropism and pathogenesis of emerging respiratory viruses, Health Research Symposium 2007.

Chen X., Smith G.J., Zhou B., Qui C., Wu W.L., Li Y., Lu P., Duan L., Liu S., Yuan J., Yang G., Wang H., Cheng J., Jiang H., Peiris J.S.M., Chen H., Yuen K.Y., Zhong N.S. and Guan Y., Avian Influenza A (H5N1) Infection in a Patient in China, 2006, Influenza and Other Respiratory Viruses. 2008, 1: 207-213.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Cheung C.L., Fan X., Smith G.J., Zhang J., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Chu K.W., Peiris J.S.M., Chen H., Guan Y. and Poon L.L.M., Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats. , J Gen Virol. 2008, 89: 1282-7.

Dhanasekaran V., Zhang J., Smith G.J., Chen H. and Guan Y., Ecology and evolution of coronaviruses, The XIth International Nidoviral Symposium St Catherine's College, Oxford, UK, 2008.

Dhanasekaran V., Wang J., Smith G.J., Peiris J.S.M. and Guan Y., Evolution of Influenza A (H5N1) virus in Asia: evidence from systematic surveillance, International Conference on Emerging and Re-emerging viral diseases of the tropics and sub-tropics organized by Indian Virological Society. 2007.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Ducatez M.F., Chen H., Guan Y. and Muller C.P., Molecular epidemiology of chicken anemia virus (CAV) in southeastern Chinese live birds markets., Avian Diseases. 2008, 52(1): 68-73.

Guan Y., Chen H., Li K.S., Riley S., Leung G.M., Webster R., Peiris J.S.M. and Yuen K.Y., A model to control the epidemic of H5N1 influenza at the source., BMC Infectious Diseases. 2007, 13;7: 132.

Guan Y., Ecology and molecular epidemiology of influenza virus, 4th Pasteur-Asia Virology Course: Influenza, SARS and Other Emerging Respiratory Viral Infections in HKU on 16 July, 2007. 2007.

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Effect of interventions on influenza a (H9N2) isolation in Hong Kong's live poultry markets, 1999-2005, Emerging Infectious Diseases. 2007, 13(9): 1340-1347.

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Minimizing risk of avian influenza: The effect of interventions on H9N2 influenza isolation in live poultry markets, 1999-2005 (abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 8 September 2007, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2007, Free Paper 5.

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Nguyen T.D., Nguyen T.V., Dhanasekaran V., Webster R.G., Guan Y., Peiris J.S.M. and Smith G.J., Multiple lineages of Influenza A (H5N1) viruses in Vietnam (2006-2007), Emerging Infectious Diseases. 2008, 14: 632-636.

Nicholls J.M., Chan M.C., Chan W.Y., Wong H.K., Cheung C.Y., Kwong D.L.W., Wong M.P., Chui W.H., Poon L.L., Tsao S.W., Guan Y. and Peiris J.S.M., Jropism of avian influenza A (H5N1) in the upper and lower respiratory tract, Nat Med. 2007, 13: 147-9.

Nicholls J.M., Bourne A.J., Chen H., Guan Y. and Peiris J.S.M., Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses, Respiratory Research. 2007, 8: 73.

Peiris J.S.M. and Guan Y., Chapter in Novartis Foundation Symposium 290 - Emerging infectious diseases and the animal-human interface , 2008 Novel and Re-emerging Respiratory Viral Diseases. 2008, 113-127.

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Fan X., Li K., Chen H., Peiris J.S.M. and Guan Y., Ecology and evolution of influenza A (H5N1) virus in Asia: Evidence from systematic influenza surveillance, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Dhanasekaran V., Bahl J. and Guan Y., Estimating dates of influenza A (H5N1) virus transmission, 6th Asia-Pacific Bioinformatics Conference, Kyoto, Japan. 2008.

Smith G.J., Guan Y. and Peiris J.S.M., Evolution of H5N1 avian influenza, 2nd Annual NIAID Centers of Excellence for Influenza Research and Surveillance Network Meeting, Memphis, TN, USA. 2008.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Xu K., Zhang J., Duan L., Smith G.J., Peiris J.S.M., Chen H. and Guan Y., Evolution of H9N2 influenza A viruses in quail from southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Zhang J., Dhanasekaran V., Liu W., Dong B., Zhu H., Li L., Smith G.J., Chen H. and Guan Y., Interspecies transmission of coronaviruses in wild animals, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Zhou B., Zhong N. and Guan Y., Treatment with convalescent plasma for influenza A (H5N1) infection., The New England journal of medicine. 2007, 357(14): 1450-1451.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Researcher : Ho PL

Project Title:	A phenotypic and genotypic analysis of vancomycin tolerance in Streptococcus pnemoniae
Investigator(s):	Ho PL, Yuen KY, Que TL
Department:	Microbiology
Source(s) of Funding:	Low Budget High Impact Programme
Start Date:	11/2000

Abstract:

To identify the frequency of occurrence of vancomycin tolerance in Streptococcus pneumoniae; to identify novel mutations in the histdine kinase (vncS) of tolerant pneumococci.

Project Title:	Molecular epidemiology of fluoroquinolone-resistant streptococcus pneumoniae in Hong kong and Canada
Investigator(s):	Ho PL
Department:	Microbiology
Source(s) of Funding:	Low Budget High Impact Programme
Start Date:	11/2001

Abstract:

To define the molecular epidemiology of fluoroquinolone-resistant strains of S. pneumoniae isolated in Hong Kong and Canada.

Project Title:	Clinical outcome and impact on health care utilization of invasive pneumococcal disease in the era of antimicrobial resistance
Investigator(s):	Ho PL, Cheng YH, Chiu SSS, Tsang KWT
Department:	Microbiology
Source(s) of Funding:	Health Care and Promotion Fund - Research Grant Projects
Start Date:	08/2002

Abstract:

To determine the impact of antibiotic resistance on patient outcome in invasive pneumococcal disease and to what extent additional health care resources are utilized as a result of the antibiotic resistance in a cohort of patients between 1997 to 2000; to document the serotypes of S. pneumoniae that casue invasive infection in the same cohort of patients.

Project Title:	Risk factors for SARS in health care workers following incubation of SARS patients
Investigator(s):	Ho PL, Hung CT
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To identify factors associated with SARS transmission in HCW who participated in intubation of SARS patients; to define the transmission probability of an intubation procedure of SARS patient and to describe the intubation procedural practice of clinicians in the first two months of the outbreak.

Project Title:	Fluoroquinolone susceptibility clinical breakpoints and fluoroquinolone-resistant determining mutations among clinical strains of streptococcus pneumoniae
Investigator(s):	Ho PL, Wong SSY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To determine the frequency of occurrence of first step fluoroquinolone-resistant determining mutations among isolates categorized as susceptible by the currently accepted clinical breakpoints.

Project Title:	Antimicrobial resistance among uropathogens causing well-defined episodes of uncomplicated cystitis among adult women in primary care settings
Investigator(s):	Ho PL, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

(1) to assess prevalence of antimicrobial resistnace among uropathogens causing well-defined episodes of acute uncomplicated cystitis in adult women; (2) to assess the clonality, resistant determinants and virulence factor profiles of antibiotic-resistant strains of uropathogenic E. coli.

Project Title:	Evaluation of molecular tests for the rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis and their impact on patient management
Investigator(s):	Ho PL, Yam WC, Leung CC, Yew WW, Mok YW, Chan KS, Tam CM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	04/2006

Abstract:

(1) to evaluate rapid tests for genotypic detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis in respiratory specimens and culture isolates; (2) to assess the effects of a model for targeted testing of patients with risks for multidrug-resistant tuberculosis on patient management

Project Title:	The epidemiological relationships between CTX-M producing Escherichia coli from animal and human sources
Investigator(s):	Ho PL, Yam WC
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2007

Abstract:

The aim of this study is to test the hypothesis that (1) there are epidemiological links between antibiotic-resistant E. coli in the three ecological compartments represented by food animals, community and hospital; (2) expansion of limited clones contribute to the observed CTX-M frequencies and that the resistant clones were derived from antibiotic-susceptible strains acquiring resistance determinants; (3) there are multiple genetic background among the gene capture units carrying the CTX-M genes and the same profile of genetic background are present among strains from animal and human.

List of Research Outputs

Chan G.C.F., Chan S.H.S., Ho P.L., Wang L. and Ha S.Y., Effects of Different Forms of Iron Chelators on Bacteria Commonly found in Asian Children, 17^th International Conference on Chelation (ICOC) for the treatment of thalassaemia, cancer and other diseases related to metal and free radical imbalance and toxicity, Shenzhen, PR China, 23-26 November 2007. 36.

Chiu S.S.S., Ho P.L., Chow K.H., Yuen K.Y. and Lau Y.L., Nasopharyngeal Carriage of Antimicrobial-Resistant Streptococcus pneumoniae among Young Children Attending 79 Kindergartens and Day Care Centers in Hong Kong, Health Research Symposium 2007: Building Bridges between Research, Practice & Policy, 29 September 2007.

Ho P.L., Antimicrobial resistance in Escherichia coli isolated from food animals and humans, M.Phil. thesis (co-supervisor) submitted by Mr. Wong Chun Wai. 2007.

Ho P.L., Poon W.W.N., Loke S.L., Leung M.S.T., Chow K.H., Wong R.C.W., Yip K.S., Lai E.L.Y. and Tsang K.W.T., Community emergence of CTX-M type extended-spectrum beta-lactamases among urinary Escherichia coli from women. , Journal of Antimicrobial Chemotherarpy. 2007, 60(1): 140-144.

Ho P.L., Epidemiology and virulence characteristics of multidrug-resistant Escherichia coli from women with acute uncomplicated cystitis, M.Phil thesis submitted by Mr. Yip King Sun. 2007.

Ho P.L., Excellent Research Award”, Food and Health Bureau, HKSAR, 09/2007 for work on antimicrobial resistance, 2007.

Ho P.L., Molecular characterization of Mycobacterium tuberculosis associated with Phenotypic Virulence in human macrophages, Ph.D. thesis (co-supervisor) submitted by Mr. K.C. Wong. 2007.

Ho P.L., Chiu S.S.S., Chow K.H., Mak C.K. and Lau Y.L., Pediatric hospitalization for pneumococcal diseases preventable by 7-valent pneumococcal conjugate vaccine in Hong Kong, Vaccine. 2007, 25: 6837-6841.

Lam B. and Ho P.L., Opportunistic pulmonary infections, In Eds. Ho JCM, Lam B, Ip MSM, Lam WK, Clinical Respiratory Medicine, 3rd Edition, 2007. Hong Kong, The Logistics & Reprographic Unit, The Registry, HKU, 2007, Chapter 11: 90-102.

Lam T.H., Yuen K.Y., Ho P.L., Wong K.C., Leong W.M., Law H.K.W., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Differential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis, Microbial Pathogenesis. 2008, 45: 12-17.

Wong K.C., Leong W.M., Law H.K.W., Ip K.F., Lam T.H., Yuen K.Y., Ho P.L., Tse W.S., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Molecular Characterization of Clinical Isolates of Mycobacterium tuberculosis and Their Association with Phenotypic Virulence in Human Macrophages, In: ASM, Clinical and Vaccine Immunology. ASM, 2007, 14(10).

Researcher : Huang K

List of Research Outputs

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Researcher : Huang Y

List of Research Outputs

Huang Y., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., CoVDB: a comprehensive detabase for comparative analysis of coronavirus genes and genomes, Nucleic Acids Research. 2007, 36: D504-D511.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Woo P.C.Y., Wong B.H.L., Huang Y., Lau S.K.P. and Yuen K.Y., Cytosine deamination and selection of CpG suppressed clones are the two major independent biological forces that shape codon usage bias in coronaviruses, Virology. 2007, 369: 431-442.

Researcher : Huang Y

List of Research Outputs

Huang Y., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., CoVDB: a comprehensive detabase for comparative analysis of coronavirus genes and genomes, Nucleic Acids Research. 2007, 36: D504-D511.

Researcher : Hui CK

Project Title:	Significance of occult hepatitis B infection in patients undergoing autologous HSCT
Investigator(s):	Hui CK, Liang RHS, Lau G, Lie AKW
Department:	Medicine
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	10/2006

Abstract:

(1) The occurrence of de novo HBV or occult HBV infection with or without clinical hepatitis after HSCT in HBsAg negative patients pre-HSCT; (2) the risk of transmitting de novo or occult HBV from occult HBV infected blood products to HBsAg negative immunocompromised patients.

List of Research Outputs

Hui C.K., Zhang H., Bowden S., Locarnini S., Luk J.M.C., Leung K.W., Yueng Y.H., Wong A., Rousseau F., Yuen K.Y., Naoumov N.N. and Lau G., 96 weeks combination of adefovir dipivoxil plus emtricitabine vs. adefovir dipivoxil monotherapy in the treatment of chronic hepatitis B, Journal of Hepatology. 2008, 48(5): 714-720.

Hui C.K., Cheung W.W., Leung K.W., Cheng V.C.C., Tang S.F., Li W.S., Lee N.P., Kwong Y.L., Au W.Y., Yuen K.Y., Lau G. and Liang R.H.S., Outcome and immune reconstitution of HBV-specific immunity in patients with reactivation of occult HBV infection after alemtuzumab-containing chemotherapy regimen, Hepatology. EPub, 2008.

Hui C.K., Leung N., Shek T.W.H., Yao H., Lee W.K., Lai J.Y., Lai S.T., Wong W.M., Lai S.L., Poon R.T.P., Lo C.M., Fan S.T. and Lau G., Sustained disease remission after spontaneous HBeAg seroconversion is associated with reduction in fibrosis progression in chronic hepatitis B Chinese patients , Hepatology. 2007, 46(3): 690-698.

Researcher : Hui PY

List of Research Outputs

Padhan K., Tanwar C., Hussain A., Hui P.Y., Lee M.Y., Cheung C.Y., Peiris J.S.M. and Jameel S., Severe Acute Respiratory Syndrome Coronavirus Orf3a Protein Interacts With Caveolin, Journal of General Virology. 2008, 88: 3067-77.

Researcher : Im SWK

Project Title:	Investigation of community exposure to a novel coronavirus associated with SARS and herd immunity developed among healthy blood donors
Investigator(s):	Im SWK, Yam WC
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To determine the exposure of the Hong Kong community to the novel coronavirus and the extent of herd immunity developed; to optimize a cost-effective protocol suitable for high throughput blood testing screening.

Project Title:	Dendritic cell based immune intervention of nasopharyngeal carcinoma
Investigator(s):	Im SWK, Zheng B, Chua DTT
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

N/A

Researcher : Jayawardena S

List of Research Outputs

Jayawardena S. and Poon L.L.M., Use of reverse transcription loop-mediated isothermal amplification for molecular detection of highly pathogenic H5N1 influenza A viruses. , The options for the control of influenza virus VI. 2007.

Researcher : Kao RYT

Project Title:	Application of chemical genetic approaches to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei
Investigator(s):	Kao RYT, Yuen KY, Sun H
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

In conjunction with conventional genetic approaches (such as gene cloning and gene inactivation, etc.), we set out to employ chemical genetic approach - the use of high-throughput screening (HTS) technologies to identify biologically active small molecules that will interfere with particular cellular processes/biological pathways/gene products - to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei. Forward chemical genetics will be used to identify small molecule compounds that will interfere with host-pathogen interactions and dimorphism in P. marneffei. Reverse chemical genetics will be used to identify small molecules that will have specific inhibitory effects on MetAP1 or MetAP2 (essential enzymes involved in removal of N-terminal methionine) of P. marneffei. Resulting specific inhibitors of MetAPs will be tested in in vitro intracellular survival and in vivo animal infection models.

Project Title:	Mode of action of a lead anti-influenza compound identified by chemical genetics
Investigator(s):	Kao RYT
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2006

Abstract:

Based on the concept of chemical genetics and in conjunction with the high-throughput screening (HTS) technologies we have developed, we set out to establish a HTS-based chemical genetic platform for the identification of biologically active small molecule compounds that will perturb most, if not all, biological pathways involved in the pathogenesis of influenza A viruses in cellular models. Forward chemical genetics has been employed to identify small molecule compounds that will interfere with infectivity of human influenza A/WSN/33 (H1N1) in Madin-Darby canine kidney (MDCK) cells. Primary screening of the library has identified more than 1,000 compounds that can perturb the infectivity of H1N1 virus in cell-based assays. A sampling survey of 70 primary hits indicates that ~10% of the hits have EC50 (median effective concentration) at or below 10 microM [determined by MTT (3-[4,5-dimethylth-iazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and PRA (plaque reduction assay)]. The result is in good agreement with the ones we obtained from our previously published SARS-CoV study. One particular lead compound FA5123 {[4-(2-Chloro-4-nitro-phenyl)-2-methyl-piperazin-1-yl]-[3-(2-chloro-phenyl)-5-methyl-isoxazol-4-yl]-methanone} has been identified (figure 1). FA5123 inhibits influenza A/WSN/33 (H1N1) in MDCK cells with an EC50 of 5 microM and TC50 (median toxicity concentration) > 1 mM [determined by MTT (3-[4,5-dimethylth-iazol-2-yl]-2,5-diphenyltetrazolium bromide)-based assay]. The molecular target of this isolated compound will be defined by isolation of mutant viruses resistant to this particular compound followed by identification of the mutated gene by sequencing of the mutant viral gene segments. It is not our major goal to produce ready-to-use, marketable antiviral agents from this study, for that is the job of the pharmaceutical companies. We are exploring novel strategies that can be employed to obtain biologically active novel small-molecule compounds (with defined molecular targets) that will have the potential of being developed to be novel anti-influenza therapeutics.

Project Title:	Methionine aminopeptidase (MetAP) as a novel target for development of antibiotic therapeutics against Staphylococcus aureus (S. aureus) - a proteomic approach
Investigator(s):	Kao RYT, Yuen KY, Che CM, Siu AFM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	02/2006

Abstract:

Using proteomic approach in conjunction with conventional genetic manipulations of S. aureus wild-type strain and its newly constructed MetAP knockout strain, we intend to gain insight into the physiological roles of MetAP. The biomarkers of MetAP deficient state in S. aureus will be studied and be used as future platform for development and validation of MetAP specific antibiotics.

Project Title:	Cloning and characterization of Streptococcus pneumoniae methionine aminopeptidase: a potential target for antimicrobial therapies
Investigator(s):	Kao RYT
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2007

Abstract:

Objectives of the proposed project: Co- and post-translational modifications have long been recognized as essential steps for the maturation, regulation, and eventually degradation of proteins. Since protein synthesis begins at N-terminus with methionine or N-formyl methionine (initiator methionine), modification at this site becomes one of the earliest and most important steps determining the fate of the translating peptide. Methionine aminopeptidases (MetAPs), the enzymes responsible for the catalytic removal of the N-terminal methionine, are defined by their highly conserved substrate specificity dictated by the residue adjacent to the initiator methionine residue. Extensive biochemical and mutational studies have confirmed that if the second residue in the primary sequence is small and uncharged (e.g. Ala, Cys, Gly, Pro, Ser, Thr, or Val), the MetAPs will then catalytically cleave the N-terminal methionine residue. There are two classes of MetAPs, designated MetAP1 (type I) and MetAP2 (type II), and both are metalloenzymes that will cleave the N-terminal methionine if the second residue is small and uncharged. Type I enzymes are found in Eubacteria while type II enzymes are found in Archaea, and methionine aminopeptidases of both types are present in eukaryotes. As Eubacteria contain only one type of methionine aminopeptidase gene (map), it has been demonstrated that deletion of this gene from E. coli and Salmonella typhimurium is lethal. Yeast expresses both map1 and map2 genes. Deletion of either one is not lethal but deletion mutant of both map1 and map2 is nonviable, indicating that N-terminal methionine removal is an essential function in both bacteria and yeast. Its physiological importance has given rise to considerable interest among researchers to design antimicrobial inhibitors that specifically target this enzyme. Analysis of map gene in S. pneumoniae: Extensive in silico studies reveal that there is only one copy of map gene in S. pneumoniae and MetAPs in S. pneumoniae and in E.coli share 33% identity in amino acid sequence (figure 1). It is generally believed that the MetAP from S. pneumoniae will have the same enzymatic activity and specificity as the E.coli counterpart as all proposed active site residues and metal ion binding motifs are conserved in the two MetAPs, though this speculation has yet to be demonstrated experimentally. Recent advancements in synthetic organic chemistry, molecular biology, and informatics have made possible the use of large collections of small molecules (chemical libraries) to investigate protein/chemical interactions in vitro and in vivo (Kao et al. 2002, 2004; Degterev et al., 2001; Peterson et al., 2000; Mayer et al., 1999). The term "chemical genetics” has been coined to signify the use of chemicals to systematically perturb, and thus determine, the function of proteins in the same way that mutations are used in classical genetics. In forward chemical genetics, small molecules that induce altered phenotypes in cells or organisms are identified and their cellular targets will then be determined subsequently. In reverse chemical genetics, small molecules that interact with a known, purified protein are identified in vitro and their effects on cells or organisms will then be examined. I intend to clone the gene methionine aminopeptidase employ high-throughput screened (HTS) based reverse chemical genetics to identify small molecules that will have specific inhibitory effects on the S. pneumoniae MetAP-I (SpMetAP-I). Resulting specific inhibitors of SpMetAP-I will be tested in cell-based assays (Streptococcus pneumoniae, Staphylococcus aureus, and E. coli) to determine their in vivo antimicrobial effects. My objectives are to: 1. Clone the gene encoding methionine aminopeptidase in Streptococcus pneumoniae R6 strain in E. coli. 2. Over-express spMetAP in E.coli host and purify the recombinant protein to homogeneity. 3. Characterize the enzymatic properties of spMetAP. 4. Cary out pilot-scale HTS of small molecule inhibitor of spMetAP. 5. Test the inhibitory effects of the anti-spMetAP compounds on S. pneumoniae, S. aureus, and E.coli in cellular assays.

List of Research Outputs

Du L., Kao R.Y.T., Zhou Y., He Y., Zhao G., Wong K.L., Jiang S., Yuen K.Y., Jin D. and Zheng B., Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity., Biochem. Biophys. Res. Commun.. 2007, 359(1): 174-179.

Tsui Y.K., Leung Y.L., Kao R.Y.T., Chan D., Masuda K. and Cheung K.M.C., High throughput screening of chemical compounds for the treatment of osteoarthritis, Best Poster Award, 12th Ressearch Postgraduate Symposium, The University of Hong Kong, December 2007. 2007.

Researcher : Lai ELY

List of Research Outputs

Ho P.L., Poon W.W.N., Loke S.L., Leung M.S.T., Chow K.H., Wong R.C.W., Yip K.S., Lai E.L.Y. and Tsang K.W.T., Community emergence of CTX-M type extended-spectrum beta-lactamases among urinary Escherichia coli from women. , Journal of Antimicrobial Chemotherarpy. 2007, 60(1): 140-144.

Researcher : Lam HY

List of Research Outputs

Chen J.H.K., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., Molecular epidemiology and divergence of HIV type 1 protease codon 35 inserted strains among treatment-naive patients in Hong Kong, In: Chen J.H.K., Wong K.H., Chan K.C., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., AIDS Research and Human Retroviruses. 2008, 24: 537-542.

Researcher : Lam SF

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Researcher : Lam SY

List of Research Outputs

Chan K.H., Yam W.C., Pang C.M., Chan K.M., Lam S.Y., Lo K.F., Poon L.L.M. and Peiris J.S.M., Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples, Journal of Clinical Microbiology. 2008, 46(7): 2195-2199.

Chan K.H., Sonnenberg K., Niedrig M., Lam S.Y., Pang C.M., Chan C.M., Ma S.K., Seto W.H. and Peiris J.S.M., Use of antibody avidity assays for diagnosis of severe acte respiratory syndrome coronavirus infection, Clinical and Vaccine Immunology. 2007, 14(11): 1433-6.

Researcher : Lam TH

List of Research Outputs

Lam T.H., Yuen K.Y., Ho P.L., Wong K.C., Leong W.M., Law H.K.W., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Differential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis, Microbial Pathogenesis. 2008, 45: 12-17.

Wong K.C., Leong W.M., Law H.K.W., Ip K.F., Lam T.H., Yuen K.Y., Ho P.L., Tse W.S., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Molecular Characterization of Clinical Isolates of Mycobacterium tuberculosis and Their Association with Phenotypic Virulence in Human Macrophages, In: ASM, Clinical and Vaccine Immunology. ASM, 2007, 14(10).

Researcher : Lau CY

List of Research Outputs

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Researcher : Lau SKP

Project Title:	Environmental surveillance of Laribacter hongkongensis, a diarrheal pathogen discovered in Hong Kong
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2004

Abstract:

To conduct a territory-wide, environmental surveillance in Hong Kong to study the epidemiology and potential environmental reservoir of L. hongkongensis.

Project Title:	Antibiotic susceptibility profiles and cloning and characterization of antibiotic resistance genes of Laribacter hongkongensis
Investigator(s):	Lau SKP, Woo PCY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	07/2005

Abstract:

To elucidate the antibiotic susceptibility profile of human and animal strains of L. hongkongensis>; to compare macrobroth dilution method, E-test and disk diffusion test for antibiotic susceptibility testing in L. hongkongensis; to clone and characterize the beta-lactamase and tetracycline resistance genes of L. hongkongensis.

Project Title:	Characterization of two novel anaerobic, gram-positive bacilli recovered from blood cultures of patients
Investigator(s):	Lau SKP, Woo PCY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

The objectives of this study are to characterize two novel anaerobic, gram-positive bacilli recovered from blood cultures of patients in Hong Kong.

Project Title:	Development of a multilocus sequence typing system for Laribacter hongkongensis
Investigator(s):	Lau SKP, Woo PCY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

Understanding the epidemiology of infectious disease pathogens is the first step in combating infectious diseases. This study will help facilitate the comparison of different strains of L. hongkongensis isolated form different hosts or the environment; and in turn, the sources of human infections and possible control measures.

Project Title:	Characterization of an unidentified anaerobic, gram-positive bacillus recovered from blood culture of a patient with acute proctitis
Investigator(s):	Lau SKP, Woo PCY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2006

Abstract:

The objective of this study is to characterize an unidentified anaerobic, gram-positive bacillus recovered from blood culture of a patient.

List of Research Outputs

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Cheng V.C.C., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection, Clinical Microbiology Reviews. 2007, 20: 660-694.

Hao W., Pan Y.X., Ding Y.Q., Xiao S., Yin K., Wang Y.D., Qiu L.W., Zhang Q.L., Woo P.C.Y., Lau S.K.P., Yuen K.Y. and Che X.Y., Well-characterized monoclonal antibodies against cell wall antigen of Aspergillus species improve immunoassay specificity and sensitivity, Clinical and Vaccine Immunology. 2008, 15: 194-202.

Huang Y., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., CoVDB: a comprehensive detabase for comparative analysis of coronavirus genes and genomes, Nucleic Acids Research. 2007, 36: D504-D511.

Lau S.K.P., Yip C.Y., Que T.L., Lee R.A., Au-yeung R.K.H., Zhou B., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong, Journal of Infectious Diseases. 2007, 196: 986-993.

Lau S.K.P., Yip C.Y., Tsoi H.W., Lee R.A., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical features and complete genome characterization of a distinct human rhinovirus genetic cluster, probably representing a previously undetected HRV species, HRV associated with acute respiratory illness in children, Journal of Clinical Microbiology. 2007, 45(11): 3655-3664.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Lau S.K.P., Wong G.K.M., Li W.S., Woo P.C.Y. and Yuen K.Y., Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis, Journal of Antimicrobial Chemotherapy. 2008, 61: 488-497.

Lau S.K.P., Diversity of coronaviruses in bats: insights into origin of SARS coronavirus., 13th International Congress on Infectious Diseases (Kuala Lumpur, Malaysia).. 2008.

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Lau S.K.P., SARS coronavirus: from human to civet and bat., UK-HK Frontiers of Science Symposium (Jointly organized by the Royal Society and Croucher Foundation) (Hong Kong). 2008.

Lau S.K.P., Sir Patrick Manson Gold Medal on Doctor of Medicine thesis, The University of Hong Kong. 2007.

Woo P.C.Y., Wong B.H.L., Huang Y., Lau S.K.P. and Yuen K.Y., Cytosine deamination and selection of CpG suppressed clones are the two major independent biological forces that shape codon usage bias in coronaviruses, Virology. 2007, 369: 431-442.

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Lau S.K.P. and Yuen K.Y., Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis, Biotechnology Letters. 2007, 29: 1575-1582.

Woo P.C.Y., Lau S.K.P., Ngan H.Y., Tse H., Tung T.K. and Yuen K.Y., Lasiodiplodia theobromae pneumonia in a liver transplant recipient, Journal of Clinical Microbiology. 2008, 46: 380-384.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, International Journal of Infectious Diseases. 2008, 12: S45.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, The 15th International Symposium on Infections in the Immunocompromised Host. 2008.

Researcher : Lau SY

List of Research Outputs

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Researcher : Lee MY

Project Title:	Role of Cyclooxygenase-2 (COX-2) in Pro-inflammatory Response of Severe Acute Respiratory Syndrome (SARS) Coronavirus-Infected Human Primary Monocyte-derived Macrophages and the Potential Use of its Inhibitors for the Therapy of SARS
Investigator(s):	Lee MY, Peiris JSM, Cheung CY, Nicholls JM
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	01/2007

Abstract:

Severe acute respiratory syndrome (SARS) emerged firstly in Guangdong Province, China in early 2003. The pathogen of SARS is a novel coronavirus named SARS-associated coronavirus (SARS-CoV) and is believed to be of zoonotic origin. Although the two well-known human coronaviruses 229E and OC43 cause only the common cold, several animal coronaviruses which may cause severe disease, can affect the respiratory or gastrointestinal tract or cause disseminated infections. Compared to common respiratory viral infections, SARS is unusually severe, with an overall case fatality rate of about 10%. The infection is not localized to the respiratory tract, and the causative virus is also detected in the gastrointestinal and urinary systems. Although the biological basis for the severity of SARS remained unclear, recent studies demonstrated that SARS coronavirus (SARS-CoV) can induce chemokines in macrophages in vitro and this has been suggested to contribute extensive inflammatory response and consequently to the pathogenicity of SARS-CoV (1, 2). Recent studies have demonstrated that the enzyme cyclooxygenase (COX) is involved in the host response to viral infections (3) and this enzyme is implicated as an important factor in inflammation modulation and immune response (4, 5). COX is a bifunctional enzyme, possessing the cyclooxygenase activity to convert arachidonic acid into prostaglandin G2 (PGG2), and the peroxidase activity to reduce PGG2 producing PGH2. PGH2 is then metabolized into the various bioactive prostanoids by their corresponding terminal synthases. COX exists as two different isoforms designated as COX-1 and COX-2. Although both isoforms are involved in the formation of PG endoperoxides, they are likely to have fundamentally different biological roles. COX-1 has the structural characteristic of a constitutively expressed gene and is found in most mammalian tissues. It is thought to be involved in maintaining physiological functions such as cytoprotection of the gastric mucosa, regulation of renal blood flow and control of platelet aggregation. In contrast, the expression of COX-2 is thought to be inducible and is upregulated by cytokines, bacterial lipopolysaccharide (LPS) and growth factors. Based on its distinctive expression profile, COX-2 is suggested to account for prostanoid formation in diseases such as inflammation and thus, it is the target for the design of the anti-inflammatory drug (6). Our microarray data (unpublished) showed that COX-2 gene was upregulated at 3 hours after SARS-CoV infection of human primary monocyte-derived macrophages. Importantly, SARS-CoV infected macrophages have 8.6 folds higher induction of COX-2 when compared to mock infected cells. This preliminary data suggest that COX-2 may play an important role in the cellular response to SARS-CoV infection. Based on the above issues, it would be of great interest to investigate the novel association of COX-2 and SARS-CoV and its role in the pathogenesis of SARS. Thus, the objectives of this proposed project are to investigate 1.) the involvement and role of COX-2 in pro-inflammatory response after SARS-CoV infection and 2.) the therapeutic potential of selective COX-2 inhibitors against SARS-CoV. References: 1) Cheung CY, Poon LL, Ng IH, Luk W, Sia SF, Wu MH, Chan KH, Yuen KY, Gordon S, Guan Y, Peiris JS. Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis. J Virol. 2005;79(12):7819-26. 2) Weiss SR, Navas-Martin S. Coronavirus pathogenesis and the emerging pathogen severe acute respiratory syndrome coronavirus. Microbiol Mol Biol Rev. 2005;69(4):635-64. 3) Carey MA, Bradbury JA, Seubert JM, Langenbach R, Zeldin DC, Germolec DR. Contrasting effects of cyclooxygenase-1 (COX-1) and COX-2 deficiency on the host response to influenza A viral infection. J Immunol. 2005;175(10):6878-84. 4) Martin JG, Suzuki M, Maghni K, Pantano R, Ramos-Barbon D, Ihaku D, Nantel F, Denis D, Hamid Q, Powell WS. The immunomodulatory actions of prostaglandin E2 on allergic airway responses in the rat. J Immunol. 2002;169(7):3963-9. 5) Carey MA, Germolec DR, Bradbury JA, Gooch RA, Moorman MP, Flake GP, Langenbach R, Zeldin DC. Accentuated T helper type 2 airway response after allergen challenge in cyclooxygenase-1-/- but not cyclooxygenase-2-/- mice. Am J Respir Crit Care Med. 2003;167(11):1509-15. 6) McAdam BF, Mardini IA, Habib A, Burke A, Lawson JA, Kapoor S, FitzGerald GA. Effect of regulated expression of human cyclooxygenase isoforms on eicosanoid and isoeicosanoid production in inflammation. J Clin Invest. 2000;105(10):1473-82.

Project Title:	Role of cyclooxygenase-2 (COX-2) influenza A (subtype H5N1) viral pathogenesis and the potential use of its inhibitors for the therapy of H5N1 disease
Investigator(s):	Lee MY, Peiris JSM, Cheung CY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2007

Abstract:

To investigate the role of COX-2 in the pathogenesis of influenza A H5N1 disease

List of Research Outputs

Lee M.Y., Cheung C.Y., Nicholls J.M. and Peiris J.S.M., Induction of COX-2 mediated proinflammatory cascade during H5N1 infection, Health Research Symposium 2007.

Padhan K., Tanwar C., Hussain A., Hui P.Y., Lee M.Y., Cheung C.Y., Peiris J.S.M. and Jameel S., Severe Acute Respiratory Syndrome Coronavirus Orf3a Protein Interacts With Caveolin, Journal of General Virology. 2008, 88: 3067-77.

Researcher : Lee RA

List of Research Outputs

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Researcher : Leung CYH

List of Research Outputs

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Imai Y., Kuba K., Neely G.G., Yaghubian-Malhami R., Perkmann T., Van Loo G., Ermolaeva M., Veldhuizen R., Leung C.Y.H., Wang H., Liu H., Sun Y., Pasparakis M., Kopf M., Mech C., Bavari S., Peiris J.S.M., Slutsky A.S., Akira S., Hultqvist M., Holmdahl R., Nicholls J.M., Jian C., Binder C.J. and Penninger J.M., Identification of oxidative stress and Toll-like receptor 4 signaling as a key pathway of acute lung injury., Cell. Elsevier, 2008, 133(2): 235-249.

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Effect of interventions on influenza a (H9N2) isolation in Hong Kong's live poultry markets, 1999-2005, Emerging Infectious Diseases. 2007, 13(9): 1340-1347.

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Minimizing risk of avian influenza: The effect of interventions on H9N2 influenza isolation in live poultry markets, 1999-2005 (abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 8 September 2007, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2007, Free Paper 5.

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Researcher : Leung KW

List of Research Outputs

Hui C.K., Cheung W.W., Leung K.W., Cheng V.C.C., Tang S.F., Li W.S., Lee N.P., Kwong Y.L., Au W.Y., Yuen K.Y., Lau G. and Liang R.H.S., Outcome and immune reconstitution of HBV-specific immunity in patients with reactivation of occult HBV infection after alemtuzumab-containing chemotherapy regimen, Hepatology. EPub, 2008.

Researcher : Li KSM

List of Research Outputs

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Researcher : Li L

List of Research Outputs

Carrington C.V.F., Foster J.E., Zhang J., Li L., Smith G.J., Thompson N., Auguste A.J., Ramkissoon V., Adesiyun A.A. and Guan Y., Group 1 coronaviruses detected in Trinidadian bats, Keystone Global Health Symposium "Molecular Evolution as a Driving Force in Infectious Diseases”, 2008, CO, USA. 2008.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Zhang J., Dhanasekaran V., Liu W., Dong B., Zhu H., Li L., Smith G.J., Chen H. and Guan Y., Interspecies transmission of coronaviruses in wild animals, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Researcher : Li OTW

List of Research Outputs

Ng S.F., Li O.T.W., Cheung K.W., Peiris J.S.M. and Poon L.L.M., Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans., Virology Journal. 2008, 5: 2.

Researcher : Li SMK

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Researcher : Li WS

List of Research Outputs

Hui C.K., Cheung W.W., Leung K.W., Cheng V.C.C., Tang S.F., Li W.S., Lee N.P., Kwong Y.L., Au W.Y., Yuen K.Y., Lau G. and Liang R.H.S., Outcome and immune reconstitution of HBV-specific immunity in patients with reactivation of occult HBV infection after alemtuzumab-containing chemotherapy regimen, Hepatology. EPub, 2008.

Lau S.K.P., Wong G.K.M., Li W.S., Woo P.C.Y. and Yuen K.Y., Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis, Journal of Antimicrobial Chemotherapy. 2008, 61: 488-497.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Lau S.K.P. and Yuen K.Y., Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis, Biotechnology Letters. 2007, 29: 1575-1582.

Researcher : Li WS

List of Research Outputs

Researcher : Li Z

List of Research Outputs

Li Z., Yao H., Ma Y., Dong Q., Peng Y., Zheng B., Huang J., Chan C.Y., Lin M.C., Sung J.J., Yuen K.Y., Kung H.F. and He M.L., Inhibition of HBV gene expression and replication by stably expressed interferon-α1 via adeno-associated viral vectors, J Gene Med.. 2008, 10(6): 619-627.

Researcher : Lin T

List of Research Outputs

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Researcher : Lin Y

List of Research Outputs

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Du L., Zhao G., Lin Y., Chan C.S., He Y., Jian S., Wu C.Y., Jin D., Yuen K.Y., Zhou Y. and Zheng B., Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection, Vaccine. 2008, 26(13): 1644-1651.

Yang N., Tanner J.A., Zheng B., He M.L., Lu L., Jiang J.Q., Shum K.T., Lin Y., Wong K.L. and Lin M.C., Bismuth Complexes Inhibit the SARS Coronvirus, Angew. Chem. Int. Ed. 2007, 46: 6464.

Yang N., Tanner J.A., Zheng B., Watt R.M., He M.L., Lu L., Jiang J., Shum K.T., Lin Y., Wong K.L., Lin M.C., Kung H.F., Sun H. and Huang J., Bismuth complexes inhibit the SARS coronavirus, Angewandte Chemie International Edition. 2007, 46: 6464-6468.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Researcher : Lin Y

List of Research Outputs

Yang N., Tanner J.A., Zheng B., He M.L., Lu L., Jiang J.Q., Shum K.T., Lin Y., Wong K.L. and Lin M.C., Bismuth Complexes Inhibit the SARS Coronvirus, Angew. Chem. Int. Ed. 2007, 46: 6464.

Researcher : Ma S

List of Research Outputs

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Researcher : Ma SK

List of Research Outputs

Chan K.H., Sonnenberg K., Niedrig M., Lam S.Y., Pang C.M., Chan C.M., Ma S.K., Seto W.H. and Peiris J.S.M., Use of antibody avidity assays for diagnosis of severe acte respiratory syndrome coronavirus infection, Clinical and Vaccine Immunology. 2007, 14(11): 1433-6.

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Researcher : Ma SSL

List of Research Outputs

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Lau S.K.P. and Yuen K.Y., Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis, Biotechnology Letters. 2007, 29: 1575-1582.

Researcher : Mak CK

List of Research Outputs

Ho P.L., Chiu S.S.S., Chow K.H., Mak C.K. and Lau Y.L., Pediatric hospitalization for pneumococcal diseases preventable by 7-valent pneumococcal conjugate vaccine in Hong Kong, Vaccine. 2007, 25: 6837-6841.

Researcher : Ng CF

List of Research Outputs

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Researcher : Ng F

List of Research Outputs

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., A feasibility study for detection of HIV specific cytotoxic T lymphocytes using rAAV delivery system. , 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention.. Sydney, Australia., 2007.

Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., A mechanism underlying susceptibility to HBV infection: HBV down-regulate transferase-1 type I interferon receptor 1 via suppressing poly(ADP-ribose), 2007 International Meeting: The Molecular Biology of Hepatitis B Viruses.. 2007.

Researcher : Ng SF

List of Research Outputs

Ng S.F., Li O.T.W., Cheung K.W., Peiris J.S.M. and Poon L.L.M., Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans., Virology Journal. 2008, 5: 2.

Researcher : Ng SP

Project Title:	Monoclonal antibodies against lipopolysaccharide antigens of Salmonella and Shigella spp.
Investigator(s):	Ng SP
Department:	Microbiology
Source(s) of Funding:	Research Initiation Programme
Start Date:	02/2001

Abstract:

To characteerise a panel of monoclonal antibodies raised against the outer core region of Salamonella lipopolysaccharides (LPS).

Project Title:	Collection of background information on the alternative faecal indicators in the environmental waters of Hong Kong and their correlation with Escherichia coli
Investigator(s):	Ng SP, Yam WC
Department:	Microbiology
Source(s) of Funding:	Environment Protection Department - General Award
Start Date:	06/2001

Abstract:

To detect and enumerate alternative faecal microbial indicator organisms in the environmental waters of Hong Kong and to correlate the results with Escherichia coli in order to assess their value in water quality monitoring.

List of Research Outputs

Leung M.K.H., Leung Y.C., Yam W.C. and Ng S.P., Light-transmitting tubular-honeycomb photocatalytic reactor, Patent, Publication no.: 1099477. 2007, 12 pages.

Sun H., Choy T.S.J., Yam W.C., Ng S.P. and Fung Y.S., Nano-silver Modified Nucleic Acid / Piezoelectric Quartz Crystal Biosensor for Assay of Low E.coli count in Water, The 10th World Congress on Biosensors . 2008, P1.158.

Sun H., Yam W.C., Ng S.P., Li Z. and Fung Y.S., Nano-silver Modified Nucleic Acid / Piezoelectric Quartz Crystal Biosensor for Detection of E. Coli in Environmental Water , Abstract of 3rd International Workshop on Biosensors for Food Safety and Environmetnal Monitoring, Fez, Morocco, October 18-20, 2007 . 2007, p41, pp71.

Researcher : Ngan HY

List of Research Outputs

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Woo P.C.Y., Lau S.K.P., Ngan H.Y., Tse H., Tung T.K. and Yuen K.Y., Lasiodiplodia theobromae pneumonia in a liver transplant recipient, Journal of Clinical Microbiology. 2008, 46: 380-384.

Researcher : Pang CM

List of Research Outputs

Chan K.H., Yam W.C., Pang C.M., Chan K.M., Lam S.Y., Lo K.F., Poon L.L.M. and Peiris J.S.M., Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples, Journal of Clinical Microbiology. 2008, 46(7): 2195-2199.

Chan K.H., Sonnenberg K., Niedrig M., Lam S.Y., Pang C.M., Chan C.M., Ma S.K., Seto W.H. and Peiris J.S.M., Use of antibody avidity assays for diagnosis of severe acte respiratory syndrome coronavirus infection, Clinical and Vaccine Immunology. 2007, 14(11): 1433-6.

Researcher : Peiris JSM

Project Title:	The application of DNA arrays to characterise the avian influenza gene pool in southern China
Investigator(s):	Peiris JSM, Guan Y, Shortridge KF
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2002

Abstract:

This project is a multi-institutional collaborative study for developing a high throughput DNA array to allow genotyping of influenza virus isolates of avian origin from Hong Kong and applying it to characterize the influenza virus gene pool in sourthern China. Through its application, it is expected to:- 1) identify the precursor virus that donated its internal genes to generate the mutiple reassortment of H5N1 isolated in Hong Kong markets in 2001; 2) define the frequency of reassortment between viruses of different virus lineages that co-circulate in poultry in Hong Kong and correlate this to host-specificity; and 3) investigate whether avian internal gene segments are transiently introduced into the human influenza gene pool.

Project Title:	Influenza pandemic preparedness in Asia (Research collaboration with national Institute of Allergy and Infectious Diseases (NIAID), St. Jude Children's Research Hospital, Inc. (SJCRH) and The University of Hong Kong)
Investigator(s):	Peiris JSM, Guan Y
Department:	Microbiology
Source(s) of Funding:	National Institutes of Health, US Department of Health and Human Services - General Award
Start Date:	04/2003

Abstract:

To study influenza pandemic preparedness in Asia.

Project Title:	Virus-cell interaction and the pathogenesis of SARS
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To explore what is the biological basis for the selective attraction of macrophages to the lung; to study what immunopathological consequences follow.

Project Title:	Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?
Investigator(s):	Peiris JSM, Guan Y, Lau ASY, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

To identify the viral genetic determinants responsible for the differential cytokine hyper-induction associated with H5N1/97 viruses. We have already established the role of the viral NS gene, however, a role for other viral genes has not been excluded. we hypothesize (and have preliminary evidence) that one or more other viral genes are also involved in the hyper-induction of cytokines; to determine whether the avian viral genes/gene products of H5N1/97 directly up-regulate cytokine induction or lack "immune evasion" mechanisms evolved by human influenza viruses? to determine whether the differential up-regulation of cytokines associated with H5N1/97 is also observed in human lung epithelial cells (primary bronchial epithelial cells and human lung epithelial cell lines e.g. A549 cells.

Project Title:	The role of influenza virus gene constellation and viral morphology on cytokine induction, pathogenesis and viral virulence
Investigator(s):	Peiris JSM, Nicholls JM, Guan Y, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	02/2005

Abstract:

To correlate the cytokine induction phenotype with viral genotype and morphology.

Project Title:	The macrophage in the pathogenesis of Severe Acute Respiratory Syndrome Coronavirus infection
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

The global gene expression profiling of SARS-CoV infected macrophages will provide the basis for a more detailed analysis of the signaling pathways involved in subsequent studies, and may provide rational and novel therapeutic options to be devised for treating SARS.

Project Title:	Mass Tag PCR: A novel, multiplex and potentially sensitive approach for the rapid detection of respiratory pathogens
Investigator(s):	Peiris JSM, Chiu SSS, Cheung CY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	06/2006

Abstract:

To divise, optimize and evaluate the Mass Tag PCR system for the rapid disgnosis for simultaneous screening of a wide spectrum of causative agents for human respiratory tract infections, including the avian influenza H5N1 and H7N7, SARS-coronavirus (SARS-CoV). Thiw will significantly facilitate better prevention, treatment and control of infectious diseases.

Project Title:	Pathogenesis, cell signaling and virus evolution of avian influenza A (H5N1)
Investigator(s):	Peiris JSM, Guan Y, Ip NY, Lau ASY, Nicholls JM, Chen H, Poon LLM, Lau YL, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Central Allocation Vote - Group Research Project
Start Date:	06/2006

Abstract:

To study the continued evolution of H5N1 influenza viruses in Vietnam and Indonesia, in the context of the ongoing studies in Hong Kong and mainland China; to define differences in cell-signaling pathways activated by avian influenza A H5N1 compared to convetional human influenza (H1N1 or H3N2) in primary human macrophages and epithelial cells; to compare the biological characteristics of H5N1 viruses from Indonesia and Vietnam to define a basis for possible differential pathogenicity for humans; investigate the biological significance of specific viral genetic mutations/changes in recent h5N1 viruses in primary human cell culture models

Project Title:	The pathogenesis of avian influenza A H5N1 in humans: macrophage and epithelial cell-virus interactions in vitro and ex-vivo
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	The Wellcome Trust - General Award
Start Date:	01/2007

Abstract:

Specific aims are to: 1) Characterise the influenza viral replication profiles in tissue explant (ex-vivo) cultures of nasopharyngeal biopsies and lung tissue and primary cell cultures of human macrophages and respiratory (nasopharyngeal, bronchial, alveolar) epithelium. 2) Charaterise the functional receptors for H5N1 virus on macrophages and nasopharyngeal epithelium. 3) Define the consequences of viral entry via alternative pathways including the entry of non-neutralised virus antibody complexes via the Fc and complement receptors, to determine how these pathways affects subsequent viral replication and /or innate immune responses such as cytokine/chemokine secretion.

Project Title:	Role of toll-like receptors and retinoic acid-inducible gene 1 in the induction of cytokines in SARS-coronavirus infected human primary macrophages
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2007

Abstract:

To investigate the role of ToII-like receptors (TLR) and retinoic acid inducible gene (RIG-1) in the induction of pro-inflammatory cytokines, chemokines and interferon stimulated genes in SARS coronavirus and other coronavirus infected primary macrophages and dendritic of human and murine origin by using a) gene knock-down (siRNA); b) gene over-expression.

List of Research Outputs

Abdel-Ghafar A.N., Chotpitayasunondh T., Gao Z., Hayden F.G., Nguyen D.H., de Jong M.D., Naghdaliyev A., Peiris J.S.M., Shindo N., Soeroso S. and Uyeki T.M., Update on avian influenza A (H5N1) virus infection in humans., In: Writing Committee of the Second World Health Organization Consultation on Clinical Aspects of Human Infection with Avian Influenza A (H5N1) Virus., The New England Journal of Medicine. 2008, 358(3): 261-73.

Al-Azemi A., Bahl J., Al-Zenki S., Al-Shayji Y., Al-Amad S., Chen H., Guan Y., Peiris J.S.M. and Smith G.J., Avian influenza A (H5N1) virus outbreaks in Kuwait, 2007, Emerging Infectious Diseases. 2008, 14: 958-961.

Chan K.H., Peiris J.S.M., Lim W., Nicholls J.M. and Chiu S.S.S., Comparison of nasopharyngeal flocked swabs and aspirates for rapid diagnosis of respiratory viruses in children , Journal of Clinical Virology. 2008, 42(1): 65-9.

Chan K.H., Yam W.C., Pang C.M., Chan K.M., Lam S.Y., Lo K.F., Poon L.L.M. and Peiris J.S.M., Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples, Journal of Clinical Microbiology. 2008, 46(7): 2195-2199.

Chan K.H., Sonnenberg K., Niedrig M., Lam S.Y., Pang C.M., Chan C.M., Ma S.K., Seto W.H. and Peiris J.S.M., Use of antibody avidity assays for diagnosis of severe acte respiratory syndrome coronavirus infection, Clinical and Vaccine Immunology. 2007, 14(11): 1433-6.

Chan M.C.W., Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y. and Peiris J.S.M., Polarity of influenza H5N1 virus infection in human respiratory epithelial cells, Health Research Symposium 2007.

Chan W.Y., Yu C.L., Nicholls J.M., Tsao G.S.W., Peiris J.S.M. and Chan M.C.W., Innate immune response and virus replication in human nasopharyngeal epithelial cells infected with influenza A (H5N1) virus, American Society for Microbiology general meeting 2008.

Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y., Peiris J.S.M. and Chan M.C.W., The use of in vitro and ex vivo model to study the tropism and pathogenesis of emerging respiratory viruses, Health Research Symposium 2007.

Chan Y.K., Ching C.Y.J., Xu M.S., Cheung A.N.Y., Yip S.P., Lam L.Y.C., Lai S.T., Chu C.M., Wong A.T.Y., Song Y., Huang F., Liu W., Chung P.H., Leung G.M., Chow E.Y.D., Chan E.Y.T., Chan J.C.K., Ngan H.Y.S., Tam P.K.H., Chan L.C., Sham P.C., Chan V.S.F., Peiris J.S.M., Lin S.C.L. and Khoo U.S., Association of ICAM3 Genetic Variant with Severe Acute Respiratory Syndrome, The Journal of Infectious Diseases. 2007, 196: 271-280.

Chen X., Smith G.J., Zhou B., Qui C., Wu W.L., Li Y., Lu P., Duan L., Liu S., Yuan J., Yang G., Wang H., Cheng J., Jiang H., Peiris J.S.M., Chen H., Yuen K.Y., Zhong N.S. and Guan Y., Avian Influenza A (H5N1) Infection in a Patient in China, 2006, Influenza and Other Respiratory Viruses. 2008, 1: 207-213.

Cheng K.Y., Cowling B.J., Ho L.M., Peiris J.S.M., Seto W.H., Yung R., Uyeki T.M., Houck P.M. and Leung G.M., Evaluation of a rapid test (QuickVue) by comparing with viral culture for detection of influenza in a sub-tropical community setting (abstract and poster presentation), 12th Research Postgraduate Symposium, 12 December 2007, Hong Kong. Hong Kong, LKS Faculty of Medicine, HKU, 2007, 110.

Cheng K.Y., Cowling B.J., Ho L.M., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Validation of the quickvue rapid diagnostic test for influenza in Hong Kong community (abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 8 September 2007, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2007, Poster 12.

Cheuk D.K.L., Tang I.W.H., Chan K.H., Woo P.C.Y., Peiris J.S.M. and Chiu S.S.S., Rhinovirus infection in hospitalized children in Hong Kong - A prospective study, The Pediatric Infectious Diseases Journal. 2007, 26: 995-1000.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Chong W.P., Ip E.W.K., Tso H.W., Ng M.W., Wong W.H.S., Law H.K.W., Peiris J.S.M. and Lau Y.L., Association of cytokine and chemokine genes polymorphism with susceptibility to SARS and severity of SARS, Health Symposium 2007, 29th September, 2007, Hong Kong. 2007.

Chu K.W., Peiris J.S.M., Chen H., Guan Y. and Poon L.L.M., Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats. , J Gen Virol. 2008, 89: 1282-7.

Cowling B.J., Cheng K.Y., Chan K.H., Fung R.O.P., Fang J., Lam C.L.H., Ho L.M., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Evaluation of the QuickVue influenza A+B rapid diagnostic test in a community setting (abstract), 6th International Conference on Emerging Infectious Diseases, 16-19 March 2008, Atlanta, USA. Atlanta, Centers for Disease Control and Prevention, 2008, 203.

Cowling B.J., Cheng K.Y., Fung R.O.P., Fang J., Lam C.L.H., Ho L.M., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Pilot findings of a cluster randomized trial of non-pharmaceutical interventions for influenza prevention in households in Hong Kong (abstract), 6th International Conference on Emerging Infectious Diseases, 16-19 March 2008, Atlanta, USA. Atlanta, Centers for Disease Control and Prevention, 2008, 84.

Cowling B.J., Fung R.O.P., Cheng K.Y., Fang J., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Pilot findings of a randomized trial of non-pharmaceutical interventions to prevent influenza transmission in households in Hong Kong (abstract), X International Symposium on Respiratory Viral Infections, 28 February - 2 March 2008, Singapore . Singapore, The Macrae Group, 2008, I-2.

Cowling B.J., Fung R.O.P., Cheng K.Y., Fang J., Chan K.H., Seto W.H., Yung R., Chiu B., Lee P., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Preliminary findings of a randomized trial of non-pharmaceutical interventions to prevent influenza transmission in households, PLoS One. 2008, 3(5): e2101.

Dhanasekaran V., Wang J., Smith G.J., Peiris J.S.M. and Guan Y., Evolution of Influenza A (H5N1) virus in Asia: evidence from systematic surveillance, International Conference on Emerging and Re-emerging viral diseases of the tropics and sub-tropics organized by Indian Virological Society. 2007.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Guan Y., Chen H., Li K.S., Riley S., Leung G.M., Webster R., Peiris J.S.M. and Yuen K.Y., A model to control the epidemic of H5N1 influenza at the source., BMC Infectious Diseases. 2007, 13;7: 132.

Haselhorst T., Garcia J., Islam T., Lai J.C.C., Rose F.J., Nicholls J.M., Peiris J.S.M. and von Itzstein M., Avian influenza H5-containing virus-like particles (VLPs): host-cell receptor specificity by STD NMR spectroscopy, Angewandte Chemie (International ed. in English). 2008, 47(10): 1910-2.

Imai Y., Kuba K., Neely G.G., Yaghubian-Malhami R., Perkmann T., Van Loo G., Ermolaeva M., Veldhuizen R., Leung C.Y.H., Wang H., Liu H., Sun Y., Pasparakis M., Kopf M., Mech C., Bavari S., Peiris J.S.M., Slutsky A.S., Akira S., Hultqvist M., Holmdahl R., Nicholls J.M., Jian C., Binder C.J. and Penninger J.M., Identification of oxidative stress and Toll-like receptor 4 signaling as a key pathway of acute lung injury., Cell. Elsevier, 2008, 133(2): 235-249.

Khoo U.S., Chan Y.K., Chan V.S.F., Ching C.Y.J., Yam L., Chu C.M., Lai S.T., Wong T.Y., Tam P.K.H., Yip S.P., Leung G.M., Lin C.L. and Peiris J.S.M., Role of polymorphisms of the inflammatory response genes and DC-SIGNR in genetic susceptibility to SARS and other infections, Hong Kong Med J. 2008, 14(4)(Suppl 4): S9-12.

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Effect of interventions on influenza a (H9N2) isolation in Hong Kong's live poultry markets, 1999-2005, Emerging Infectious Diseases. 2007, 13(9): 1340-1347.

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Minimizing risk of avian influenza: The effect of interventions on H9N2 influenza isolation in live poultry markets, 1999-2005 (abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 8 September 2007, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2007, Free Paper 5.

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., SARS-coronavirus induced different gene expression of chemokine receptors in human monocyte-derived adult and cord blood dendritic cells, The 3^rd Congress of Asian Society for Pediatric Research, Tokyo, Japan, 6-9 October 2007. 103.

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., The role of dendritic cells in SARS coronavirus infection, Health Symposium 2007, 29th September, 2007, Hong Kong. 2007.

Lee D.C.W., Mok K.P., Law H.Y., Tam H.M., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., Mechanisms of interferon dysregulation and virus-induced cell death in Avian-Influenza H5N1 infections, Health Research Symposium 2007: Building bridges between research, practice & policy, September 29,. 2007.

Lee M.Y., Cheung C.Y., Nicholls J.M. and Peiris J.S.M., Induction of COX-2 mediated proinflammatory cascade during H5N1 infection, Health Research Symposium 2007.

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Mao H., Zhou J., Tu W., Law H.K.W., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Differential expressions of cytokines and chemokines in macrophages infected with 483/97 and 1203/04 H5N1 viruses, The 3^rd Congress of Asian Society for Pediatric Research, Tokyo, Japan, 6-9 October 2007. 104.

Mao H., Zhou J., Tu W., Law H.K.W., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Differential proinflammatory responses in macrophages infected with 483/97 and 1203/04 H5N1 viruses, 12nd Research Postgraduate Symposium, LKS Faculty of Medicine, The University of Hong Kong, 12-14 December, 2007. 114.

Mao H., Tu W., Qin G., Chan P.L., Law H.K.W., Zheng J., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Enhanced activation and cytotoxicity of natural killer cells by influenza H5N1 virus-infected macrophages, Hong Kong Society for Immunology 2008 Annual General Meeting and Scientific Meeting, 19th April, 2008, HKSAR. 2008, 38.

Ng S.F., Li O.T.W., Cheung K.W., Peiris J.S.M. and Poon L.L.M., Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans., Virology Journal. 2008, 5: 2.

Nguyen T.D., Nguyen T.V., Dhanasekaran V., Webster R.G., Guan Y., Peiris J.S.M. and Smith G.J., Multiple lineages of Influenza A (H5N1) viruses in Vietnam (2006-2007), Emerging Infectious Diseases. 2008, 14: 632-636.

Nicholls J.M. and Peiris J.S.M., Avian influenza: update on pathogenesis and laboratory diagnosis, Respirology. 2008, 13(Suppl 1): S14-S18.

Nicholls J.M., Chan W.Y., Russell R.J., GM A.I.R. and Peiris J.S.M., Evolving complexities of influenza virus and its receptors, Trends in Microbiology. 2008, 16(4): 149-157.

Nicholls J.M. and Peiris J.S.M., Ex vivo tissues and emerging viral infections, International Consortium for Antivirals, Toronto, Canada. 2008.

Nicholls J.M., Chan M.C., Chan W.Y., Wong H.K., Cheung C.Y., Kwong D.L.W., Wong M.P., Chui W.H., Poon L.L., Tsao S.W., Guan Y. and Peiris J.S.M., Jropism of avian influenza A (H5N1) in the upper and lower respiratory tract, Nat Med. 2007, 13: 147-9.

Nicholls J.M., Bourne A.J., Chen H., Guan Y. and Peiris J.S.M., Sialic acid receptor detection in the human respiratory tract: evidence for widespread distribution of potential binding sites for human and avian influenza viruses, Respiratory Research. 2007, 8: 73.

Nicholls J.M. and Peiris J.S.M., The use of ex-vivo tissues for studying infection by emerging viruses, Bangkok International Conference on Avian Influenza, 23-25 January 2008.. 2008.

Padhan K., Tanwar C., Hussain A., Hui P.Y., Lee M.Y., Cheung C.Y., Peiris J.S.M. and Jameel S., Severe Acute Respiratory Syndrome Coronavirus Orf3a Protein Interacts With Caveolin, Journal of General Virology. 2008, 88: 3067-77.

Peiris J.S.M., "Meet the Professor" Workshops - Lecture: Diagnostic tools in respiratory infections - when to use, what to ask for., 26th Annual Meeting of the European Society for Paediatric Infectious Diseases (ESPID), Graz, Austria, May 13-16, 2008. 2008.

Peiris J.S.M., Adventures in Virology - A symposium for Robin Weiss - Lecture: Confronting Emerging Diseases, Division of Infection & Immunity, University College London, United Kingdom, 20-21 Sept. 2007. 2007.

Peiris J.S.M., Biochemical, functional and immunogeneic characterisation of the SARS Spike glycoprotein: Implications for the development of a subunit vaccine, PhD. student Mr. Kam Yiu Wing. 2007.

Peiris J.S.M., Chairman for Session: Determinants of the species barrier, ESNIP Minisymposium. Gent, Belgium. 4-5 Jun. 2008. 2008.

Peiris J.S.M., Chairman for Sesson: Avian influenza in pigs, ESNIP Minisymposium. Gent, Belgium, 4-5 Jun. 2008. 2008.

Peiris J.S.M., Chairman of the Conference, X International Symposium on Respiratory Viral Infections Meeting, Singapore. 2008.

Peiris J.S.M. and Guan Y., Chapter in Novartis Foundation Symposium 290 - Emerging infectious diseases and the animal-human interface , 2008 Novel and Re-emerging Respiratory Viral Diseases. 2008, 113-127.

Peiris J.S.M., Convener: New Viruses: Old Diseases?, 47th ICAAC, Chicago, U.S.A. 17-20 Sept. 2007. 2007.

Peiris J.S.M., Convener: Viral Pathogenesis Symposium, 47th ICAAC, Chicago, U.S.A. 17-20 Sept. 2007. 2007.

Peiris J.S.M., ESPID Scientific Session: New Challenges in viral infections: Lecture: Emerging Viral Infections, 26th Annual Meeting of the European Society for Paediatric Infectious Diseases (ESPID), Graz, Austria, 13-16 May, 2008. 2008.

Peiris J.S.M., Guest lecture: confronting emerging viral infections: Lessons learnt from Avian Flu and SARS, Warwick, United Kingdom. Silver Jubilee Celebrations 1982-2007 - Sri Lankan Medical and Dental Association in the UK. 2007.

Peiris J.S.M., Immunopathogenesis of H5N1 influenza, National Health Research Institutes, Tainan, Taiwan, 30 Aug. 2007. 2007.

Peiris J.S.M., International Public Health: the Need for Anti-virals. Session One: Pandemic threat: Lecture: Genetic evolution of H5N1, ICAV 6th International Symposium. Toronto, 3-6 May, 2008. 2008.

Peiris J.S.M., Main Symposium - Infectious diseases: "Immunology of viral infection". Lecture: Pathogenesis of Avian Influenza (h5N1), 13th International Congress of Immunology (ICI), Rio de Janeiro, Brazil, 21-25 Aug. 2007. 2007.

Peiris J.S.M., Moderator: Respiratory Viruses Slide Session, 47th ICAAC, Chicago, U.S.A. Sept. 17-20, 2007. 2007.

Peiris J.S.M., Multiplex reverse transcripton-PCR for detection and identification of human Parainfluenza viruses 1, 2, 3 and 4 infection in hospitalized children with respiratory disease in Hong Kong, MMedSci thesis submitted by Ms. Lam Siu Yan. 2007.

Peiris J.S.M., Plenary 1 Lecture: Challenge of Emerging Infections , Sri Lanka Medical Association 121st Annual Scientific sessions, Colombo, Sri Lanka, 19-22 Mar. 2008. 2008.

Peiris J.S.M., Plenary 4 Lecture: Avian 'flu: A threat to human health?, Sri Lanka Medical Association 121st Annual Scientific sessions, Colombo, Sri Lanka, 19-22 Mar. 2008. 2008.

Peiris J.S.M., Plenary Lecture: Why surveillance of animal influenza viruses, Bangkok International Conference on Avian Influenza 2008. Bangkok, Thailand, 23-25 Jan. 2008. 2008.

Peiris J.S.M., Prevalence of H9N2 influenza A viruses in poultry in Souther China: implications for the emergence of a new pandemic influenza, Co-supervisor of Ph.D. student: Xu Kemin. 2007. 2007.

Peiris J.S.M., Session II: Origins of Zoonotic Pathogens: Lecture: SARS Coronavirus and Avian Flu H5N1, X International Symposium on Respiratory Viral Infections Meeting, Singapore, 28 Feb. - 2 Mar. 2008. 2008.

Peiris J.S.M., Special Lecture: Avian Influenza H5N1: is it a cause for concern?, ESNIP Minisymposium. Gent, Belgium, 4-5 June, 2008. 2008.

Peiris J.S.M., Virology / Epidemiology Session. Keynote speaker, Institut Pasteur. Paris, Frances, 26-27 Frances. 2008.

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Scott J.A., Brooks W.A., Peiris J.S.M., Holtzman D. and Mulhollan E.K., Pneumonia research to reduce childhood mortality in the development world, The Journal of Clinical Investigation. 2008, 118(4): 1291-1300.

Smith G.J., Fan X., Li K., Chen H., Peiris J.S.M. and Guan Y., Ecology and evolution of influenza A (H5N1) virus in Asia: Evidence from systematic influenza surveillance, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Guan Y. and Peiris J.S.M., Evolution of H5N1 avian influenza, 2nd Annual NIAID Centers of Excellence for Influenza Research and Surveillance Network Meeting, Memphis, TN, USA. 2008.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Wong C.M., Thach T.Q., Thomas G.N., Peiris J.S.M., Chau Y.K. and Chan K.P., Assessing the confounding effects of influenza in association between air pollutants and mortality and hospitalization (abstract), 19th Conference of the International Society for Environmental Epidemiology, 5-9 September 2007, Mexico City. Mexico City, International Society for Environmental Epidemiology, 2007, E552-E553.

Wong C.M., Lai H.K., Ou C., Ho D.S.Y., Chan K.P., Thach T.Q., Yang L., Chau Y.K., Lam T.H., Hedley A.J. and Peiris J.S.M., Is exercise protective against influenza-associated mortality?, PLoS One. 2008, 3(5): e2108.

Wong C.M., Yang L., Chan K.P., Chiu S.S.S., Lam T.H., Hedley A.J. and Peiris J.S.M., Modeling the disease burden of influenza in the topical/subtropical regions (powerpoint presentation), WPRO Informal Consultation on Protocols for Influenza Disease burden Studies 2007, 12-13 November 2007, Manila. Manila, WHO/WPRO, 2007.

Wong C.M., Thach T.Q., Chau Y.K., Chan K.P., Chung R.Y.N., Ou C., Yang L., Peiris J.S.M., Thomas G.N., Lam T.H., Wong T.W. and Hedley A.J., Public health and air pollution in Asia (PAPA) - A multi-city study for short-term effects of air pollution on mortality (poster presentation), Health Effects Institute Annual Conference, 27-29 April 2008, Pennsylvania. Pennsylvania, USA, Health Effects Institute, 2008.

Wong C.M., Thach T.Q., Chau Y.K., Chan K.P., Ou C., Yang L., Peiris J.S.M., Thomas G.N., Wong T.W., Lam T.H., Hedley A.J., Vichit-Vadakan N., Vajanapoom N. and Ostro B., Public health and air pollution in Asia (PAPA): A multi-city study for short-term effects of air pollution on mortality (abstract), International Conference on Environment and Health 2007, 6-8 December 2007, Hong Kong. Hong Kong, World Association of Chinese Public Health Professionals, 2007, 49-50.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Xu K., Zhang J., Duan L., Smith G.J., Peiris J.S.M., Chen H. and Guan Y., Evolution of H9N2 influenza A viruses in quail from southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Yang L., Wong C.M., Lau E.H.Y., Chan K.P., Ou C. and Peiris J.S.M., Synchrony of clinical and laboratory surveillance for influenza in Hong Kong, PLoS ONE. 2008, 3(1): e1399.

Yang L., Wong C.M., Lau E.H.Y. and Peiris J.S.M., Syndhronous oscillation of influenza virus activity and influenza-like illness (abstract and oral presentation), 12th Research Postgraduate Symposium, 14 December 2007, Hong Kong. Hong Kong, LKS Faculty of Medicine, HKU, 2007, 151.

Researcher : Poon KM

List of Research Outputs

Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., A mechanism underlying susceptibility to HBV infection: HBV down-regulate transferase-1 type I interferon receptor 1 via suppressing poly(ADP-ribose), 2007 International Meeting: The Molecular Biology of Hepatitis B Viruses.. 2007.

Researcher : Poon LLM

Project Title:	Reverse genetic for a coronavirus responsible for severe acute respiratory syndrome
Investigator(s):	Poon LLM, Guan Y
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To generate a coronavirus-like mini vRNA GFP (green fluorescent protein) reporter system to study the transcription and replication of coronavirus; to establish a reverse genetic system of coronavirus.

Project Title:	Roles of segment-specific vRNA sequences of influenza A virus in viral replication, transcription and gene expression
Investigator(s):	Poon LLM, Guan Y, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2003

Abstract:

To establish a GFP reporter assay for influenza replication studies; to determine the potential function of segment-specific viral RNA sequences in viral replication, tansporation and gene expression; to study the 5' and 3' segment-specific regions of avian, human and swine influenza.

Project Title:	Splicing of influenza A virus
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2004

Abstract:

To study the sequence requirements for the splicing of influenza viral mRNA; to study the role of splicing in controlling viral protein expression; to generate recombinant influenza viruses with mutations in the splicing sequences and determine the global effects of mutations in these regions in an influenza life cycle; to test whether there is a host-specific selective pressure on the 5' splicing site of influenza mRNA.

Project Title:	Development of one-step reverse trasncriptase-loop mediated isothermal amplification for direct detections of H5N1 and human influenza viruses
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	04/2006

Abstract:

Introduction A diagnosis of influenza A virus could be made by classical methods such as virus isolation, haemagglutinin inhibition test, antigen detection and serology. In the last decade, the rapid advancement of molecular techniques has opened up a new horizon for clinical diagnosis of influenza virus. Molecular techniques, such as PCR, mutliplex PCR, real-time PCR and nucleic acid sequence-based amplification (NASBA) are shown to be useful for clinical applications. These molecular techniques are demonstrated to be highly sensitive and specific. More importantly, most of the above molecular tests could produce diagnostic results in a very short period of time, thereby making these molecular approaches become attractive tools for rapid diagnosis of influenza viruses. These molecular tests, however, often require sophisticated equipment, expensive reagents and highly skilled personnel. Thus, these rapid molecular tests might not be the method of choice in basic clinical settings in developing countries or in field situations. The recent invention of loop-mediated isothermal amplification (LAMP) has opened up a new horizon for molecular diagnosis (1). Briefly, this method depends on auto-cycling strand displacement DNA synthesis performed by a Bst DNA polymerase. The reaction relies on recognition of the target sequence by six independent sequences in the initial stage and by four independent sequences during the later stages of the LAMP reaction, thereby making this kind of assays to be highly specific. The amplification products are stem-loop DNA structures with several inverted repeats of the target and form cauliflower-like structures with multiple loops. This approach is extreme rapid and has DNA amplification efficiency equivalent to that of PCR-based methods (1). More importantly, this approach is extreme simple and all reactions are performed in an isothermal environment. Furthermore, no special apparatus is needed, which makes it more economical and practical than nested PCR or real-time PCR. These advantages make LAMP become very applicable in very basic healthcare/field settings. Recently, using the LAMP approach, we developed several molecular assays for detecting cDNA from several pathogens (2-4). In these previous studies, the performances of these assays were at least comparable to routine molecular diagnostic tests. More importantly, we successfully used this technology for a direct detection DNA of Plasomodium falciparum in heat-treated blood (5). More importantly, our modifications of the assays could reduce the reagent cost to as low as 2 HK dollars per test (5). We expect the low running cost of this detection approach would be an attractive option for molecular diagnosis. However, our modified technology still cannot be used for a direct detection of RNA species in clinical samples. The recent outbreaks of H5 influenza viruses in Southeast Asia suggest a new pandemic influenza strain will evolve from this geographical region. Based on the experiences from other outbreaks (e.g. SARS), the availabilities of rapid diagnostic tests for H5N1 influenza virus will play a key role in patient management and policy making. We therefore reason that, due to its low running cost, the LAMP assay might be used as a screening test for extensive influenza surveillance/diagnosis. In particular, we expect this assays might be adopted by other developing countries, thereby allowing more extensive H5N1 surveillances in these countries. Here, we propose to develop a one-step reverse transcriptase (RT)-LAMP assay that allows direct detection sof RNA species from clinical samples. Aims In this study, we aim at: 1. developing a general one-step RT-LAMP protocol that allows direct detections of RNA species from clinical samples; 2. developing one-step RT-LAMP assays for avian H5N1 influenza A virus; 3 developing one-step RT-LAMP assays for human influenza A virus. References 1. Notomi et al, 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28:E63. 2. Poon et al, 2004. Rapid detection of the severe acute respiratory syndrome (SARS) coronavirus by a loop-mediated isothermal amplification assay. Clin Chem. 50:1050. 3. Poon et al, 2005. Detection of human influenza A viruses by loop-mediated isothermal amplification. J Clin Microbiol. 43:427. 4. Poon et al, 2005. Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. J Clin Microbiol. 43:3457. 5. Poon et al, A sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. In press.

Project Title:	Studies of mammalian, avian and chimeric influenza polymerase complexes
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2007

Abstract:

To determine the transcription and replication profiling of influenza viruses with avian, mammalian, or mammalian-avian chimeric polymerase complexes in human and avian cells; to test the compatibility between avian and mammalian influenza polymerase subunits; to identify the important residues in avian PB2, PB1 and PA for host adaptation; to determine the PB2-PB1 and PB1-PA interactions in mammalian-avian chimeric complexes; to identify the host factors that might interact with viral polymerase complexes for viral transcription and replication.

List of Research Outputs

Chan K.H., Yam W.C., Pang C.M., Chan K.M., Lam S.Y., Lo K.F., Poon L.L.M. and Peiris J.S.M., Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples, Journal of Clinical Microbiology. 2008, 46(7): 2195-2199.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Chu K.W., Peiris J.S.M., Chen H., Guan Y. and Poon L.L.M., Genomic characterizations of bat coronaviruses (1A, 1B and HKU8) and evidence for co-infections in Miniopterus bats. , J Gen Virol. 2008, 89: 1282-7.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Jayawardena S. and Poon L.L.M., Use of reverse transcription loop-mediated isothermal amplification for molecular detection of highly pathogenic H5N1 influenza A viruses. , The options for the control of influenza virus VI. 2007.

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Ng S.F., Li O.T.W., Cheung K.W., Peiris J.S.M. and Poon L.L.M., Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans., Virology Journal. 2008, 5: 2.

Poon L.L.M., Bat guano— the gold mine for better understanding of the evolution of coronaviruses?, XIth International Symposium of Nidoviruses. 2008.

Poon L.L.M., Emergying Nidovirus, In: Stanley Perlman, Thomsas Gasllsgher, Eric J Snijder, Nidoviruses. ASM Press, 2008, 409-418.

Poon L.L.M., Influenza RNA Polymerase as target for therapeutics , 4th Pasteur-Asia Virology Course a training course, Hong Kong, 2006 . 2007.

Poon L.L.M., Molecular Diagnosis of respiratory viruses, 4th Pasteur-Asia Virology Course a training course, Hong Kong, 2006 . 2007.

Poon L.L.M., SARS and other “new” coronaviruses., In: Thiel V, Coronaviruses: Molecular and cellular biology.. UK, Caister Academic Press, 2007, 287-298.

Researcher : Qin K

List of Research Outputs

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Researcher : Que TL

List of Research Outputs

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Researcher : Rayner JM

List of Research Outputs

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Researcher : Seto WH

List of Research Outputs

Chan K.H., Sonnenberg K., Niedrig M., Lam S.Y., Pang C.M., Chan C.M., Ma S.K., Seto W.H. and Peiris J.S.M., Use of antibody avidity assays for diagnosis of severe acte respiratory syndrome coronavirus infection, Clinical and Vaccine Immunology. 2007, 14(11): 1433-6.

Cheng K.Y., Cowling B.J., Ho L.M., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Validation of the quickvue rapid diagnostic test for influenza in Hong Kong community (abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 8 September 2007, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2007, Poster 12.

Cowling B.J., Cheng K.Y., Chan K.H., Fung R.O.P., Fang J., Lam C.L.H., Ho L.M., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Evaluation of the QuickVue influenza A+B rapid diagnostic test in a community setting (abstract), 6th International Conference on Emerging Infectious Diseases, 16-19 March 2008, Atlanta, USA. Atlanta, Centers for Disease Control and Prevention, 2008, 203.

Cowling B.J., Cheng K.Y., Fung R.O.P., Fang J., Lam C.L.H., Ho L.M., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Pilot findings of a cluster randomized trial of non-pharmaceutical interventions for influenza prevention in households in Hong Kong (abstract), 6th International Conference on Emerging Infectious Diseases, 16-19 March 2008, Atlanta, USA. Atlanta, Centers for Disease Control and Prevention, 2008, 84.

Cowling B.J., Fung R.O.P., Cheng K.Y., Fang J., Chan K.H., Seto W.H., Yung R., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Pilot findings of a randomized trial of non-pharmaceutical interventions to prevent influenza transmission in households in Hong Kong (abstract), X International Symposium on Respiratory Viral Infections, 28 February - 2 March 2008, Singapore . Singapore, The Macrae Group, 2008, I-2.

Cowling B.J., Fung R.O.P., Cheng K.Y., Fang J., Chan K.H., Seto W.H., Yung R., Chiu B., Lee P., Uyeki T.M., Houck P.M., Peiris J.S.M. and Leung G.M., Preliminary findings of a randomized trial of non-pharmaceutical interventions to prevent influenza transmission in households, PLoS One. 2008, 3(5): e2101.

Ho J.W.C., Seto W.H., Shum N.F., Wong S.M. and Choi K.Y., Clinical management pathway for elective colonic resection: the Queen Mary Hospital's experience, 2nd International Conference of the Asia Pacific Society for Healthcare Quality 2008, Singapore, 17-19 January 2008. 2007.

Li Y., Ching W.H., Qian H., Yuen P.L., Seto W.H., Kwan J.K., Leung J.K.C., Leung M.K.H. and Yu S.C.T., An evaluation of the ventilation performance of new SARS isolation wards in nine hospitals in Hong Kong, Indoor and Built Environment. Sage Publications, 2007, 16: 400-410.

Li Y., Leung M.K.H., Seto W.H., Yuen P.L., Leung J.K.C., Kwan J.K. and Yu S.C.T., Factors affecting ventilation effectiveness in SARS wards, Hong Kong Medical Journal. Hong Kong Academy of Medicine, 2008, 14 (Suppl. 1): S33-6.

Qian H., Li Y., Huang X., Sun H., Seto W.H. and Yuen P.L., Particle removal efficiency of a portable hepa air cleaner in a hospital ward, Presented in the 6th International Indoor Air Quality, Ventilation and Energy Conservation in Buildings Conference (IAQVEC 2007), Sendai, October 28th - 30th, 2007.. 2007.

Researcher : Shiu SYW

Project Title:	sPDZD2-induced protein profile changes in prostate cancer
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To express and purify sPDZD2 in E. coli, and to study the effects of the recombinant protein on the protein profiles of human prostate cancer to cells using a 'proteomics' approach.

Project Title:	Overexpression of sPDZD2 in prostate cancer cells
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Small Project Funding
Start Date:	10/2005

Abstract:

PDZ (PSD95, Discs-large, and ZO-1) domain containing protein 3 (PDZK3), also known as PDZ domain containing protein 2 (PDZD2), is a ubiquitously expressed protein of unknown function which has six PDZ domains. The protein is cleaved in a caspase-dependent mechanism to generate a secreted protein (sPDZD2), which may participate in extracellular signaling [Yeung et al. 2003, EMBO Rep 4: 412]. Interestingly, gene expression of PDZK3 has been reported to be upregulated in prostate cancer when compared with benign tissues Thus, it is believed that PDZK3, also named as activated in prostate cancer protein (AIPC), may play an important role in prostate tumorigenesis [Chaib et al. 2001, Cancer Res 61:2390]. Given that our ongoing studies have demonstrated that sPDZD2 protein is produced and secreted by human prostate cancer cell lines, we would like to postulate that sPDZD2 protein, a cleavage product of PDZD2, may play an important role in prostate cancer pathogenesis by modulating prostate cancer cell growth and differentiation. The objectives of this proposal are to 1) over-express sPDZD2 protein in prostate cancer cells, and 2) to study any effect of the protein on the expression of cell cycle and apoptosis-related proteins in the transfected cancer cells.

Project Title:	Structure-function studies of sPDZD2 protein and identification of its cellular targets
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2007

Abstract:

Globally, prostate cancer has become the third most common cancer in men, with half a million new cases each year, amounting to about 10% of all male cancers. It is the most common cancer after skin cancer, and is the second leading cause of cancer-related death in men in the United States. The emergence of prostate cancer as a public health problem in developed countries has put tremendous pressure on the healthcare system to provide new and effective treatments. While there is significant research progress on understanding the signaling mechanisms of androgen, a key growth-promoting hormone, in prostate cancer initiation and progression, advanced prostate cancer patients still suffer from inevitable relapse of the disease after receiving the recommended treatment of androgen deprivation therapy (ADT). Clearly, there is an unmet clinical need to develop other novel therapeutic agents which can act effectively alone and/ or in combination with ADT to halt or reverse the progression of advanced prostate cancer. Such demand has fueled the search for novel endo-/para-/auto-crine growth-promoting and growth-inhibitory signaling pathways important in prostate cancer pathogenesis, which may yield new therapeutic agents or targets for anti-prostate cancer drug discovery. Recently, our group has identified the human sPDZD2 protein as a novel autocrine prostate tumor suppressor [Tam et al. Endocrinology 2006;147:5023-5033]. In this project we propose to construct a genetically-modified form of the endogenous human sPDZD2 protein with prostate cancer growth-inhibitory actions on both hormone-sensitive and hormone-insensitive prostate cancer cells. To further maximize the therapeutic potential of this endogenous sPDZD2 protein and to fuel the anti-prostate cancer drug development pipeline by the target-driven approach, efforts would be expended towards the molecular identification of the cellular targets, including the receptors, acted upon by the native and genetically-modified forms of human sPDZD2 protein.

Researcher : Shortridge KF

List of Research Outputs

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Researcher : Sia SF

List of Research Outputs

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., SARS-coronavirus induced different gene expression of chemokine receptors in human monocyte-derived adult and cord blood dendritic cells, The 3^rd Congress of Asian Society for Pediatric Research, Tokyo, Japan, 6-9 October 2007. 103.

Law H.K.W., Cheung C.Y., Sia S.F., Chan Y.O., Peiris J.S.M. and Lau Y.L., The role of dendritic cells in SARS coronavirus infection, Health Symposium 2007, 29th September, 2007, Hong Kong. 2007.

Mao H., Zhou J., Tu W., Law H.K.W., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Differential expressions of cytokines and chemokines in macrophages infected with 483/97 and 1203/04 H5N1 viruses, The 3^rd Congress of Asian Society for Pediatric Research, Tokyo, Japan, 6-9 October 2007. 104.

Mao H., Zhou J., Tu W., Law H.K.W., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Differential proinflammatory responses in macrophages infected with 483/97 and 1203/04 H5N1 viruses, 12nd Research Postgraduate Symposium, LKS Faculty of Medicine, The University of Hong Kong, 12-14 December, 2007. 114.

Mao H., Tu W., Qin G., Chan P.L., Law H.K.W., Zheng J., Cheung C.Y., Sia S.F., Peiris J.S.M. and Lau Y.L., Enhanced activation and cytotoxicity of natural killer cells by influenza H5N1 virus-infected macrophages, Hong Kong Society for Immunology 2008 Annual General Meeting and Scientific Meeting, 19th April, 2008, HKSAR. 2008, 38.

Researcher : Smith GJ

List of Research Outputs

Al-Azemi A., Bahl J., Al-Zenki S., Al-Shayji Y., Al-Amad S., Chen H., Guan Y., Peiris J.S.M. and Smith G.J., Avian influenza A (H5N1) virus outbreaks in Kuwait, 2007, Emerging Infectious Diseases. 2008, 14: 958-961.

Campitelli L., Di Martino A., Spagnolo D., Smith G.J., Di Trani L., Facchini M., De Marco M.A., Foni E., Chiapponi C., Martin A.M., Chen H., Guan Y., Delogu M. and Donatelli I., Molecular analysis of avian H7 influenza viruses circulating in Eurasia in 1999–2005: detection of multiple reassortant virus genotypes, Journal of General Virology. 2008, 89: 48-59.

Carrington C.V.F., Foster J.E., Zhang J., Li L., Smith G.J., Thompson N., Auguste A.J., Ramkissoon V., Adesiyun A.A. and Guan Y., Group 1 coronaviruses detected in Trinidadian bats, Keystone Global Health Symposium "Molecular Evolution as a Driving Force in Infectious Diseases”, 2008, CO, USA. 2008.

Chen X., Smith G.J., Zhou B., Qui C., Wu W.L., Li Y., Lu P., Duan L., Liu S., Yuan J., Yang G., Wang H., Cheng J., Jiang H., Peiris J.S.M., Chen H., Yuen K.Y., Zhong N.S. and Guan Y., Avian Influenza A (H5N1) Infection in a Patient in China, 2006, Influenza and Other Respiratory Viruses. 2008, 1: 207-213.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Cheung C.L., Fan X., Smith G.J., Zhang J., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Dhanasekaran V., Zhang J., Smith G.J., Chen H. and Guan Y., Ecology and evolution of coronaviruses, The XIth International Nidoviral Symposium St Catherine's College, Oxford, UK, 2008.

Dhanasekaran V., Wang J., Smith G.J., Peiris J.S.M. and Guan Y., Evolution of Influenza A (H5N1) virus in Asia: evidence from systematic surveillance, International Conference on Emerging and Re-emerging viral diseases of the tropics and sub-tropics organized by Indian Virological Society. 2007.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Nguyen T.D., Nguyen T.V., Dhanasekaran V., Webster R.G., Guan Y., Peiris J.S.M. and Smith G.J., Multiple lineages of Influenza A (H5N1) viruses in Vietnam (2006-2007), Emerging Infectious Diseases. 2008, 14: 632-636.

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Associate Editor, Influenza and Other Respiratory Viruses. 2007.

Smith G.J., Chris Baldock Prize for Early Career Researcher, Australian Biosecurity CRC. 2007.

Smith G.J., Fan X., Li K., Chen H., Peiris J.S.M. and Guan Y., Ecology and evolution of influenza A (H5N1) virus in Asia: Evidence from systematic influenza surveillance, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Smith G.J., Ecology and evolution of influenza A (H5N1) virus in Asia, Multinational Influenza Seasonal Mortality Study (MISMS) Asia Regional Meeting (FIC/NIH), National Institute of Hygiene and Epidemiology, Hanoi, Vietnam. 2007.

Smith G.J., Dhanasekaran V., Bahl J. and Guan Y., Estimating dates of influenza A (H5N1) virus transmission, 6th Asia-Pacific Bioinformatics Conference, Kyoto, Japan. 2008.

Smith G.J., Guan Y. and Peiris J.S.M., Evolution of H5N1 avian influenza, 2nd Annual NIAID Centers of Excellence for Influenza Research and Surveillance Network Meeting, Memphis, TN, USA. 2008.

Smith G.J., Evolution of H5N1 influenza virus, Mini-Symposium on Strategy of Avian Influenza Prevention, Bureau of Animal and Plant Health Inspection and Quarantine, Council of Agriculture, Executive Yuan, Taipei, Taiwan. 2007.

Smith G.J., PLoS Pathogens, 2008.

Smith G.J., Population dynamics of avian influenza A virus, Frontiers in Biomedical Research HKU 2007, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong. 2007.

Smith G.J., Summary of SE Asia H5N1 outbreaks to date, Avian Influenza Virus Genomics and Bioinformatics Symposium (WHO/FAO/CDC/NIH), Hanoi School of Public Health, Hanoi, Vietnam. 2007.

Su Y.C.F., Smith G.J. and Saunders R.M.K., Phylogeny of the basal angiosperm genus Pseuduvaria (Annonaceae) inferred from five chloroplast DNA regions, with interpretation of morphological character evolution., Molecular Phylogenetics and Evolution. 2008, 48: 188-206.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Xu K., Zhang J., Duan L., Smith G.J., Peiris J.S.M., Chen H. and Guan Y., Evolution of H9N2 influenza A viruses in quail from southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Zhang J., Dhanasekaran V., Liu W., Dong B., Zhu H., Li L., Smith G.J., Chen H. and Guan Y., Interspecies transmission of coronaviruses in wild animals, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Researcher : Song W

List of Research Outputs

Liu N., Song W., Wang P., Lee K., Chan W., Chen H. and Cai Z., Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells., Proteomics. 2008, 8: 1851-1858.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Researcher : Sui H

List of Research Outputs

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Researcher : Tai H

List of Research Outputs

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Researcher : Tang SF

List of Research Outputs

Hui C.K., Cheung W.W., Leung K.W., Cheng V.C.C., Tang S.F., Li W.S., Lee N.P., Kwong Y.L., Au W.Y., Yuen K.Y., Lau G. and Liang R.H.S., Outcome and immune reconstitution of HBV-specific immunity in patients with reactivation of occult HBV infection after alemtuzumab-containing chemotherapy regimen, Hepatology. EPub, 2008.

Researcher : Tang X

List of Research Outputs

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Researcher : Teng LL

Project Title:	Usefulness of full sequence and 5' end 527-bp 16S ribosomal RNA gene sequencing for identification of medically important aerobic Gram-positive cocci
Investigator(s):	Teng LL, Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2006

Abstract:

To study usefulness of full sequence and 5' end 527-bp 16S ribosomal RNA gene sequencing for identification of medically important aerobic Gram-positive cocci.

List of Research Outputs

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Lau S.K.P. and Yuen K.Y., Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis, Biotechnology Letters. 2007, 29: 1575-1582.

Researcher : Tsang CL

List of Research Outputs

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Researcher : Tsang DNC

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Researcher : Tsang THF

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Researcher : Tse H

Project Title:	Phenotypic and genotypic characterization of Helcococcus isolates in Hong Kong and their mechanisms of erythromycin resistance
Investigator(s):	Tse H, Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To perform phenotypic and molecular characterization on the Helcococcus isolates from patients in Hong Kong and investigate its mechanism of erythromycin resistance

Project Title:	Characterization of genes horizontally transferred from prokaryotes to Penicillium marneffei
Investigator(s):	Tse H, Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2006

Abstract:

To study the pattern of horizontal gene transfer from prokaryotes to the fungus Penicillium marneffei and perform genetic and phylogenetic characterizations of the transferred genes, which would provide useful information about its natural habitat and its ecology in the natural environment

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Woo P.C.Y., Lau S.K.P., Ngan H.Y., Tse H., Tung T.K. and Yuen K.Y., Lasiodiplodia theobromae pneumonia in a liver transplant recipient, Journal of Clinical Microbiology. 2008, 46: 380-384.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, International Journal of Infectious Diseases. 2008, 12: S45.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, The 15th International Symposium on Infections in the Immunocompromised Host. 2008.

Researcher : Tsoi HW

List of Research Outputs

Lau S.K.P., Yip C.Y., Tsoi H.W., Lee R.A., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical features and complete genome characterization of a distinct human rhinovirus genetic cluster, probably representing a previously undetected HRV species, HRV associated with acute respiratory illness in children, Journal of Clinical Microbiology. 2007, 45(11): 3655-3664.

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Researcher : Tung TK

List of Research Outputs

Tung T.K., Cheng C.W. and Lim B.L., Stabilization of beta-propeller phytase by introducing Xaa→Pro and Gly→Ala substitutions at consensus positions. , Protein and Peptide Letter . 2008, 15: 297-299.

Woo P.C.Y., Lau S.K.P., Ngan H.Y., Tse H., Tung T.K. and Yuen K.Y., Lasiodiplodia theobromae pneumonia in a liver transplant recipient, Journal of Clinical Microbiology. 2008, 46: 380-384.

Researcher : Tung TK

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Ngan H.Y., Tse H., Tung T.K. and Yuen K.Y., Lasiodiplodia theobromae pneumonia in a liver transplant recipient, Journal of Clinical Microbiology. 2008, 46: 380-384.

Researcher : Wang J

List of Research Outputs

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Dhanasekaran V., Wang J., Smith G.J., Peiris J.S.M. and Guan Y., Evolution of Influenza A (H5N1) virus in Asia: evidence from systematic surveillance, International Conference on Emerging and Re-emerging viral diseases of the tropics and sub-tropics organized by Indian Virological Society. 2007.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Researcher : Wang J

List of Research Outputs

Researcher : Wang P

List of Research Outputs

Liu N., Song W., Wang P., Lee K., Chan W., Chen H. and Cai Z., Proteomics analysis of differential expression of cellular proteins in response to avian H9N2 virus infection in human cells., Proteomics. 2008, 8: 1851-1858.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Researcher : Wang P

List of Research Outputs

Researcher : Webster RG

List of Research Outputs

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Duan L., Campitelli L., Smith G.J., Bahl J., Donatelli I., Webster R.G., Shortridge K.F., Peiris J.S.M., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Nguyen T.D., Nguyen T.V., Dhanasekaran V., Webster R.G., Guan Y., Peiris J.S.M. and Smith G.J., Multiple lineages of Influenza A (H5N1) viruses in Vietnam (2006-2007), Emerging Infectious Diseases. 2008, 14: 632-636.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Researcher : Wong BHL

List of Research Outputs

Woo P.C.Y., Wong B.H.L., Huang Y., Lau S.K.P. and Yuen K.Y., Cytosine deamination and selection of CpG suppressed clones are the two major independent biological forces that shape codon usage bias in coronaviruses, Virology. 2007, 369: 431-442.

Researcher : Wong CK

List of Research Outputs

Leung C.Y.H., Smith G.J., Chow C.K., Ng C.F., Tsang C.L., Wong C.K., Poon L.L.M., Guan Y. and Peiris J.S.M., Influenza viruses in wild birds in Hong Kong , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Researcher : Wong GKM

List of Research Outputs

Lau S.K.P., Wong G.K.M., Li W.S., Woo P.C.Y. and Yuen K.Y., Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis, Journal of Antimicrobial Chemotherapy. 2008, 61: 488-497.

Researcher : Wong KC

List of Research Outputs

Lam T.H., Yuen K.Y., Ho P.L., Wong K.C., Leong W.M., Law H.K.W., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Differential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis, Microbial Pathogenesis. 2008, 45: 12-17.

Wong K.C., Leong W.M., Law H.K.W., Ip K.F., Lam T.H., Yuen K.Y., Ho P.L., Tse W.S., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Molecular Characterization of Clinical Isolates of Mycobacterium tuberculosis and Their Association with Phenotypic Virulence in Human Macrophages, In: ASM, Clinical and Vaccine Immunology. ASM, 2007, 14(10).

Researcher : Wong KH

List of Research Outputs

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., A feasibility study for detection of HIV specific cytotoxic T lymphocytes using rAAV delivery system. , 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention.. Sydney, Australia., 2007.

Researcher : Wong KL

List of Research Outputs

Du L., Kao R.Y.T., Zhou Y., He Y., Zhao G., Wong K.L., Jiang S., Yuen K.Y., Jin D. and Zheng B., Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity., Biochem. Biophys. Res. Commun.. 2007, 359(1): 174-179.

Yang N., Tanner J.A., Zheng B., Watt R.M., He M.L., Lu L., Jiang J., Shum K.T., Lin Y., Wong K.L., Lin M.C., Kung H.F., Sun H. and Huang J., Bismuth complexes inhibit the SARS coronavirus, Angewandte Chemie International Edition. 2007, 46: 6464-6468.

Researcher : Wong SSY

Project Title:	Molecular characterization of streptococcus bovis bacteraemia
Investigator(s):	Wong SSY, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To characterize 48 S. bovis strains isolated from the blood cultures of 37 bacteraemic patients over a six-year period using a combination of phenotypic and genotypic techniques. The epidemiology, underlying diseases, clinical disease associations, and outcome of S. bovis bacteraemia in relation to the different biotypes and genotypes of S. bovis will be investigated, and the molecular epidemiology of erythromycin resistance in the S. bovis strains will also be analyzed.

Project Title:	Screening antigenic proteins in penicillium marneffei for seroepidemiology and vaccination
Investigator(s):	Wong SSY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

To screen for yeast- and mould-phase-specific antigens that are useful for epidemiological and diagnostic purposes; to screen for yeast-phase- and mould-phase-specific antigens that induce active immunity against penicilliosis marneffei from normalized cDNA libraries of the two phases of the dimorphic fungus; to construct a prophylactic and/or therapeutic vaccine prototype; to study the seroepidemiology of penicilliosis marneffei.

Project Title:	Characterization of bacterial isolates with ambiguous biochemical profiles recovered from patients with clinically significant bacteremia by 16S rRNA gene sequencing, the state-of-the-art molecular technique for bacterial identification
Investigator(s):	Wong SSY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To use the state-of-the-art molecular technique for bacterial identification, 16S rRNA gene sequencing, to characterize these bacterial isolates. Novel bacterial species discovered will be further characterized.

Project Title:	Phenotypic and genotypic characterization of Streptococcus iniae isolates in Hong Kong
Investigator(s):	Wong SSY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To perform phenotypic and molecular characterization on the Streptococcus iniae isolates from patients in Hong Kong.

List of Research Outputs

Tiwari A.F.Y., Tarrant A.M., Yuen F.K.H., Chan S.S.C., Kagan S., Ching P., Wong A. and Wong S.S.Y., Best of Journal of Nursing Scholarship, World Health Category, Sigma Theta Tau International. 2007.

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Researcher : Woo PCY

Project Title:	Molecular epidemiology of erythromycin resistant [beta]-hemolytic Lancefield group G streptococci causing bacteremia
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To perform phenotypic and genotypic characterization of the erythromycin resistance in [beta]-hemolytic group G streptococci recovered from blood cultures of patients in a six-year period. Risk factors for bacteremia caused by [beta]-hemolytic group G streptocci will also be analyzed.

Project Title:	The role of a novel bacterium, Laribacter hongkongensis, in gastroenteritis, and its epidemiology and pathogenesis
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To study the association of L. hongkongensis with gastroenteritis in humans and the epidemiology of this novel disease entity; to provide clinicians with appropriate treatment regimen by correlation of antibiotic susceptibilities and clinical outcome; to discover the source and reservoir of the bacterium; to define its pathogenesis in terms of toxin production, tissue and animal invasiveness.

Project Title:	Usefulness of full sequence and 5' end 527-bp 16S ribosomal RNA gene sequencing for identification of medically important anaerobic bacteria
Investigator(s):	Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To study usefulness of full sequence and 5' end 527-bp 16S ribosomal RNA gene sequencing for identification of medically important anaerobic bacteria.

Project Title:	A prospective study on the epidemiology of coronavirus-associated respiratory tract infections in Hong Kong and the role of rapid disgnostic tests
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

The present study will generate valuable information for understanding the prevalence, the clinical spectrum of disease and epidemiology of the different coronaviruses in all age groups, and in turn their public health significance and economic implications, as compared to other respiratory viral pathogens. Rapid tests useful for the detection of the various coronaviruses from clinical specimens will also be developed.

Project Title:	Characterization of an unidentified gram-positive bacillus recovered from surgical site abscess of a patient with carcinoma of oesophagus.
Investigator(s):	Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2006

Abstract:

The objective of this study is to characterize an unidentified gram-positive bacillus recovered from the surgical abscess of a patient.

List of Research Outputs

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Cheng V.C.C., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection, Clinical Microbiology Reviews. 2007, 20: 660-694.

Cheuk D.K.L., Tang I.W.H., Chan K.H., Woo P.C.Y., Peiris J.S.M. and Chiu S.S.S., Rhinovirus infection in hospitalized children in Hong Kong - A prospective study, The Pediatric Infectious Diseases Journal. 2007, 26: 995-1000.

Hao W., Pan Y.X., Ding Y.Q., Xiao S., Yin K., Wang Y.D., Qiu L.W., Zhang Q.L., Woo P.C.Y., Lau S.K.P., Yuen K.Y. and Che X.Y., Well-characterized monoclonal antibodies against cell wall antigen of Aspergillus species improve immunoassay specificity and sensitivity, Clinical and Vaccine Immunology. 2008, 15: 194-202.

Huang Y., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., CoVDB: a comprehensive detabase for comparative analysis of coronavirus genes and genomes, Nucleic Acids Research. 2007, 36: D504-D511.

Lau S.K.P., Yip C.Y., Que T.L., Lee R.A., Au-yeung R.K.H., Zhou B., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong, Journal of Infectious Diseases. 2007, 196: 986-993.

Lau S.K.P., Yip C.Y., Tsoi H.W., Lee R.A., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical features and complete genome characterization of a distinct human rhinovirus genetic cluster, probably representing a previously undetected HRV species, HRV associated with acute respiratory illness in children, Journal of Clinical Microbiology. 2007, 45(11): 3655-3664.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Lau S.K.P., Wong G.K.M., Li W.S., Woo P.C.Y. and Yuen K.Y., Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis, Journal of Antimicrobial Chemotherapy. 2008, 61: 488-497.

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Woo P.C.Y., Associate Editor, Emerging Health Threats Journal, 2008.

Woo P.C.Y., Wong B.H.L., Huang Y., Lau S.K.P. and Yuen K.Y., Cytosine deamination and selection of CpG suppressed clones are the two major independent biological forces that shape codon usage bias in coronaviruses, Virology. 2007, 369: 431-442.

Woo P.C.Y., Development of a multi-locus sequence typing scheme for Laribacter hongkongensis, a novel bacterium associated with freshwater fish-borne gastroenteritis and traveler's diarrhea, revealed "pathogenic" strains, 13th International Congress for Infectious Diseases (Kuala Lumpur, Malaysia). 2008.

Woo P.C.Y., Impact of Research on the Control and Management of Infectious Diseases, Health Research Symposium 2007.

Woo P.C.Y., Invited Hong Kong Representative, United Kingdom-Hong Kong Frontiers of Science Symposium (Hong Kong). 2008.

Woo P.C.Y., Keynote lecture for Outstanding Young Researcher Awardee: Complete genome sequence of Laribacter hongkongensis, Frontiers of Biomedical Research (Hong Kong). 2007.

Woo P.C.Y., Laribacter hongkongensis, a novel bacterium associated with gastroenteritis and traveler's diarrhea, Pearl River Fisheries Research Institute (Guangzhou, China). 2008.

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Woo P.C.Y., More and More Human and Animal Coronaviruses: Comparative Genome Analysis Facilitates Discovery of Potential Novel Drug Targets, BIT's 1st Annual Protein and Peptide Conference (Shenzhen, China). 2008.

Woo P.C.Y., More and more human and animal coronaviruses, Pathology COC Commissioned Training Programme 2007/2008 (Hong Kong). 2007.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Lau S.K.P. and Yuen K.Y., Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis, Biotechnology Letters. 2007, 29: 1575-1582.

Woo P.C.Y., Lau S.K.P., Ngan H.Y., Tse H., Tung T.K. and Yuen K.Y., Lasiodiplodia theobromae pneumonia in a liver transplant recipient, Journal of Clinical Microbiology. 2008, 46: 380-384.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, International Journal of Infectious Diseases. 2008, 12: S45.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, The 15th International Symposium on Infections in the Immunocompromised Host. 2008.

Researcher : Wu AKL

List of Research Outputs

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Researcher : Wu H

List of Research Outputs

Zhang H., Wang Y.X., Wu H., Zhang Q., Wen Y.M. and Zheng B., Mutations of the turn between helix H and I of HIV-1 reverse transcriptase affect RT incorporation into viral particles and subsequent viral replication , 25 years of HIV.. 2008.

Researcher : Wu WL

List of Research Outputs

Chen X., Smith G.J., Zhou B., Qui C., Wu W.L., Li Y., Lu P., Duan L., Liu S., Yuan J., Yang G., Wang H., Cheng J., Jiang H., Peiris J.S.M., Chen H., Yuen K.Y., Zhong N.S. and Guan Y., Avian Influenza A (H5N1) Infection in a Patient in China, 2006, Influenza and Other Respiratory Viruses. 2008, 1: 207-213.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Wu W.L., Chen Y., Wang P., Song W., Lau S.Y., Rayner J.M., Smith G.J., Webster R.G., Peiris J.S.M., Lin T., Xia N., Guan Y. and Chen H., Antigenic profile of avian H5N1 viruses in Asia from 2002–2007, Journal of Virology. 2007, 82: 1798-1807.

Researcher : Xu K

List of Research Outputs

Xu K., Zhang J., Duan L., Smith G.J., Peiris J.S.M., Chen H. and Guan Y., Evolution of H9N2 influenza A viruses in quail from southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Researcher : Xu K

List of Research Outputs

Researcher : Yam WC

List of Research Outputs

Chan K.H., Yam W.C., Pang C.M., Chan K.M., Lam S.Y., Lo K.F., Poon L.L.M. and Peiris J.S.M., Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 Nucleic Acid Extraction Systems for Detection of RNA and DNA Respiratory Viruses in Nasopharyngeal Aspirate Samples, Journal of Clinical Microbiology. 2008, 46(7): 2195-2199.

Chen J.H.K., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., Molecular epidemiology and divergence of HIV type 1 protease codon 35 inserted strains among treatment-naive patients in Hong Kong, In: Chen J.H.K., Wong K.H., Chan K.C., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., AIDS Research and Human Retroviruses. 2008, 24: 537-542.

Choy T.S.J., Yam W.C., Yao S.Z. and Fung Y.S., Improved Detection Sensitivity of the Nucleic Acid / Piezoelectric Quartz Crystal Biosesor Through Nano-god Application, Abstract of 3rd International Workshop on Biosensors for Food Safety and Environmetnal Monitoring, Fez, Morocco, October 18-20, 2007. P-36, pp66.

Lam T.H., Yuen K.Y., Ho P.L., Wong K.C., Leong W.M., Law H.K.W., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Differential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis, Microbial Pathogenesis. 2008, 45: 12-17.

Leung M.K.H., Leung Y.C., Yam W.C. and Ng S.P., Light-transmitting tubular-honeycomb photocatalytic reactor, Patent, Publication no.: 1099477. 2007, 12 pages.

Sun H., Choy T.S.J., Yam W.C., Ng S.P. and Fung Y.S., Nano-silver Modified Nucleic Acid / Piezoelectric Quartz Crystal Biosensor for Assay of Low E.coli count in Water, The 10th World Congress on Biosensors . 2008, P1.158.

Sun H., Yam W.C., Ng S.P., Li Z. and Fung Y.S., Nano-silver Modified Nucleic Acid / Piezoelectric Quartz Crystal Biosensor for Detection of E. Coli in Environmental Water , Abstract of 3rd International Workshop on Biosensors for Food Safety and Environmetnal Monitoring, Fez, Morocco, October 18-20, 2007 . 2007, p41, pp71.

Wong K.C., Leong W.M., Law H.K.W., Ip K.F., Lam T.H., Yuen K.Y., Ho P.L., Tse W.S., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Molecular Characterization of Clinical Isolates of Mycobacterium tuberculosis and Their Association with Phenotypic Virulence in Human Macrophages, In: ASM, Clinical and Vaccine Immunology. ASM, 2007, 14(10).

Researcher : Yip CY

List of Research Outputs

Lau S.K.P., Yip C.Y., Que T.L., Lee R.A., Au-yeung R.K.H., Zhou B., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong, Journal of Infectious Diseases. 2007, 196: 986-993.

Lau S.K.P., Yip C.Y., Tsoi H.W., Lee R.A., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical features and complete genome characterization of a distinct human rhinovirus genetic cluster, probably representing a previously undetected HRV species, HRV associated with acute respiratory illness in children, Journal of Clinical Microbiology. 2007, 45(11): 3655-3664.

Researcher : Yip KS

List of Research Outputs

Ho P.L., Poon W.W.N., Loke S.L., Leung M.S.T., Chow K.H., Wong R.C.W., Yip K.S., Lai E.L.Y. and Tsang K.W.T., Community emergence of CTX-M type extended-spectrum beta-lactamases among urinary Escherichia coli from women. , Journal of Antimicrobial Chemotherarpy. 2007, 60(1): 140-144.

Researcher : Yu CL

List of Research Outputs

Chan M.C.W., Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y. and Peiris J.S.M., Polarity of influenza H5N1 virus infection in human respiratory epithelial cells, Health Research Symposium 2007.

Chan W.Y., Yu C.L., Nicholls J.M., Tsao G.S.W., Peiris J.S.M. and Chan M.C.W., Innate immune response and virus replication in human nasopharyngeal epithelial cells infected with influenza A (H5N1) virus, American Society for Microbiology general meeting 2008.

Chan W.Y., Yu C.L., Nicholls J.M., Chui W.H., Guan Y., Peiris J.S.M. and Chan M.C.W., The use of in vitro and ex vivo model to study the tropism and pathogenesis of emerging respiratory viruses, Health Research Symposium 2007.

Researcher : Yuen KY

Project Title:	Establishment of core facilities for the HKU HIV/AIDS research laboratories
Investigator(s):	Yuen KY, Zheng B, Woo PCY, Yam WC, Im SWK
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	11/2001

Abstract:

To support the development of state-of-art clinical management of HIV/AIDS founded on scientific research and clinical trials; to undertake research for expanding the knowledge base on HIV molecular epidemiology in Hong Kong and the reigon; to examine the immunological mechanisms underlying HIV pathogenesis; to study AIDS associated infectious diseases; to build expertise in HIV molecular biology and immunology.

Project Title:	Molecular characterization of Streptococcus milleri causing infective endocarditis
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To identify members of the "S. milleri group" in patients with infective endocarditis by 16S rRNA gene sequencing; to define the epidemiology, clinical spectrum, treatment, and outcome of infective endocarditis associated with the "S. milleri group".

Project Title:	An oral mucosal DNA vaccine for SARS coronavirus infections
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	09/2004

Abstract:

To test the feasibility of using the live-attenuated Salmonella system for delivery of DNA vaccine of the spike protein of SARS-CoV in mice, and compare the immunogenicity of oral DNA vaccine, naked DNA vaccine, and recombinant protein vaccine.

Project Title:	Characterisation of local/emerging pathogens and development of anti-infective agents
Investigator(s):	Yuen KY, Jin LJ, Kao RYT, Lau G, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Seed Funding for Strategic Research Theme
Start Date:	05/2005

Abstract:

There are 4 categories of pathogens are selected to compete with the rest of the world and therefore advance the research of our University to top international level: hepatitis B virus (HBV), Laribacter Hongkongenesis, influenze A virus and novel oral pathogens.

Project Title:	The role of a novel coronavirus, coronavirus HKU1, in respiratory illness and its epidemiology and molecular evolution
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	07/2005

Abstract:

To study the role of CoV-HKU1 in respiratory illness in humans and its clinical spectrum of disease and epidemiology; to develop laboratory tests for the diagnosis of CoV-HKU1 infection; to study the molecular evolution of this novel coronavirus.

Project Title:	Bacteraemia caused by Anaerotruncus colihominis and emended description of the species
Investigator(s):	Yuen KY, Lau SKP, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To characterize a strain of Anaerotruncus colihominis recovered from the blood culture of a patient in Hong Kong and provide an emended description of the species.

Project Title:	Wild animal surveillance for coronavirus HKU1, a novel coronavirus associated with pneumonia in patients in Hong Kong, and potential variants of other coronaviruses that infect human
Investigator(s):	Yuen KY, Lau SKP, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

The present study is going to yield valuable information for understanding the reservoir of coronavirus HKU1 and potential variants of other coroanviruses that infect human, which is crucial for the control of respiratory tract infections associated with the viruses.

Project Title:	A multilocus sequence typing system for Penicillum marneffei: an international molecular cyber system for tracking the origin and transmission of Penicillium marneffei
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

In this project, using the sequence data of the Penicillium marneffei Genome Project, we propose to build up a multilocus sequence typing (MLST) system, for this fungus.

List of Research Outputs

Chen J.H.K., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., Molecular epidemiology and divergence of HIV type 1 protease codon 35 inserted strains among treatment-naive patients in Hong Kong, In: Chen J.H.K., Wong K.H., Chan K.C., Lam H.Y., Yuen K.Y., Cheng V.C.C. and Yam W.C., AIDS Research and Human Retroviruses. 2008, 24: 537-542.

Chen X., Smith G.J., Zhou B., Qui C., Wu W.L., Li Y., Lu P., Duan L., Liu S., Yuan J., Yang G., Wang H., Cheng J., Jiang H., Peiris J.S.M., Chen H., Yuen K.Y., Zhong N.S. and Guan Y., Avian Influenza A (H5N1) Infection in a Patient in China, 2006, Influenza and Other Respiratory Viruses. 2008, 1: 207-213.

Cheng V.C.C., Wu A.K.L., Cheung C.H.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Li K.S.M., Ip I.K.S., Dunn E.L.W., Lee R.A., Yam L.Y.C. and Yuen K.Y., Outbreak of human metapneumovirus infection in psychiatric inpatients: implications for directly observed use of alcohol hand rub in prevention of nosocomial outbreaks, Journal of Hospital Infection. 2007, 67: 336-343.

Cheng V.C.C., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Severe acute respiratory syndrome coronavirus as an agent of emerging and reemerging infection, Clinical Microbiology Reviews. 2007, 20: 660-694.

Chiu S.S.S., Ho P.L., Chow K.H., Yuen K.Y. and Lau Y.L., Nasopharyngeal Carriage of Antimicrobial-Resistant Streptococcus pneumoniae among Young Children Attending 79 Kindergartens and Day Care Centers in Hong Kong, Health Research Symposium 2007: Building Bridges between Research, Practice & Policy, 29 September 2007.

Du L., Kao R.Y.T., Zhou Y., He Y., Zhao G., Wong K.L., Jiang S., Yuen K.Y., Jin D. and Zheng B., Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity., Biochem. Biophys. Res. Commun.. 2007, 359(1): 174-179.

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Du L., Zhao G., Lin Y., Chan C.S., He Y., Jian S., Wu C.Y., Jin D., Yuen K.Y., Zhou Y. and Zheng B., Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection, Vaccine. 2008, 26(13): 1644-1651.

Guan Y., Chen H., Li K.S., Riley S., Leung G.M., Webster R., Peiris J.S.M. and Yuen K.Y., A model to control the epidemic of H5N1 influenza at the source., BMC Infectious Diseases. 2007, 13;7: 132.

Hao W., Pan Y.X., Ding Y.Q., Xiao S., Yin K., Wang Y.D., Qiu L.W., Zhang Q.L., Woo P.C.Y., Lau S.K.P., Yuen K.Y. and Che X.Y., Well-characterized monoclonal antibodies against cell wall antigen of Aspergillus species improve immunoassay specificity and sensitivity, Clinical and Vaccine Immunology. 2008, 15: 194-202.

Huang Y., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., CoVDB: a comprehensive detabase for comparative analysis of coronavirus genes and genomes, Nucleic Acids Research. 2007, 36: D504-D511.

Hui C.K., Zhang H., Bowden S., Locarnini S., Luk J.M.C., Leung K.W., Yueng Y.H., Wong A., Rousseau F., Yuen K.Y., Naoumov N.N. and Lau G., 96 weeks combination of adefovir dipivoxil plus emtricitabine vs. adefovir dipivoxil monotherapy in the treatment of chronic hepatitis B, Journal of Hepatology. 2008, 48(5): 714-720.

Hui C.K., Cheung W.W., Leung K.W., Cheng V.C.C., Tang S.F., Li W.S., Lee N.P., Kwong Y.L., Au W.Y., Yuen K.Y., Lau G. and Liang R.H.S., Outcome and immune reconstitution of HBV-specific immunity in patients with reactivation of occult HBV infection after alemtuzumab-containing chemotherapy regimen, Hepatology. EPub, 2008.

Lam T.H., Yuen K.Y., Ho P.L., Wong K.C., Leong W.M., Law H.K.W., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Differential fadE28 expression associated with phenotypic virulence of Mycobacterium tuberculosis, Microbial Pathogenesis. 2008, 45: 12-17.

Lau S.K.P., Yip C.Y., Que T.L., Lee R.A., Au-yeung R.K.H., Zhou B., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical and molecular epidemiology of human bocavirus in respiratory and fecal samples from children in Hong Kong, Journal of Infectious Diseases. 2007, 196: 986-993.

Lau S.K.P., Yip C.Y., Tsoi H.W., Lee R.A., So L.Y., Lau Y.L., Chan K.H., Woo P.C.Y. and Yuen K.Y., Clinical features and complete genome characterization of a distinct human rhinovirus genetic cluster, probably representing a previously undetected HRV species, HRV associated with acute respiratory illness in children, Journal of Clinical Microbiology. 2007, 45(11): 3655-3664.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Lau S.K.P., Wong G.K.M., Li W.S., Woo P.C.Y. and Yuen K.Y., Distribution and molecular characterization of tetracycline resistance in Laribacter hongkongensis, Journal of Antimicrobial Chemotherapy. 2008, 61: 488-497.

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Li Z., Yao H., Ma Y., Dong Q., Peng Y., Zheng B., Huang J., Chan C.Y., Lin M.C., Sung J.J., Yuen K.Y., Kung H.F. and He M.L., Inhibition of HBV gene expression and replication by stably expressed interferon-α1 via adeno-associated viral vectors, J Gene Med.. 2008, 10(6): 619-627.

Wong K.C., Leong W.M., Law H.K.W., Ip K.F., Lam T.H., Yuen K.Y., Ho P.L., Tse W.S., Weng X.H., Zhang W.H., Chen S. and Yam W.C., Molecular Characterization of Clinical Isolates of Mycobacterium tuberculosis and Their Association with Phenotypic Virulence in Human Macrophages, In: ASM, Clinical and Vaccine Immunology. ASM, 2007, 14(10).

Woo P.C.Y., Wong B.H.L., Huang Y., Lau S.K.P. and Yuen K.Y., Cytosine deamination and selection of CpG suppressed clones are the two major independent biological forces that shape codon usage bias in coronaviruses, Virology. 2007, 369: 431-442.

Woo P.C.Y., Lau C.Y., Chong T.K., Tse H., Tsang D.N.C., Lee R.A., Tse C.W.S., Que T.L., Chung L.M.W., Ngan H.Y., Hui W.T., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., MP1 homologue-based multilocus sequence system for typing the pathogenic fungus Penicillium marneffei: a novel approach using lineage-specific genes, Journal of Clinical Microbiology. 2007, 45: 3647-3654.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Lau S.K.P. and Yuen K.Y., Plasmid profile and construction of a small shuttle vector in Laribacter hongkongensis, Biotechnology Letters. 2007, 29: 1575-1582.

Woo P.C.Y., Lau S.K.P., Ngan H.Y., Tse H., Tung T.K. and Yuen K.Y., Lasiodiplodia theobromae pneumonia in a liver transplant recipient, Journal of Clinical Microbiology. 2008, 46: 380-384.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., A feasibility study for detection of HIV specific cytotoxic T lymphocytes using rAAV delivery system. , 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention.. Sydney, Australia., 2007.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, International Journal of Infectious Diseases. 2008, 12: S45.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, The 15th International Symposium on Infections in the Immunocompromised Host. 2008.

Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., A mechanism underlying susceptibility to HBV infection: HBV down-regulate transferase-1 type I interferon receptor 1 via suppressing poly(ADP-ribose), 2007 International Meeting: The Molecular Biology of Hepatitis B Viruses.. 2007.

Researcher : Zhang H

List of Research Outputs

Wang Y.X., Zhang H., Xu J., Wen Y.M. and Zheng B., Turn residue at the helix clamp motif of reverse transcriptase is crucial for replication for both hepatitis B virus and human immunodeficiency virus , 2007 International Meeting on the Molecular Biology of Hepatitis B Viruses. 2007.

Zhang H., Wang Y.X., Wu H., Zhang Q., Wen Y.M. and Zheng B., Mutations of the turn between helix H and I of HIV-1 reverse transcriptase affect RT incorporation into viral particles and subsequent viral replication , 25 years of HIV.. 2008.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Researcher : Zhang J

List of Research Outputs

Carrington C.V.F., Foster J.E., Zhang J., Li L., Smith G.J., Thompson N., Auguste A.J., Ramkissoon V., Adesiyun A.A. and Guan Y., Group 1 coronaviruses detected in Trinidadian bats, Keystone Global Health Symposium "Molecular Evolution as a Driving Force in Infectious Diseases”, 2008, CO, USA. 2008.

Cheung C.L., Dhanasekaran V., Smith G.J., Fan X.H., Zhang J., Bahl J., Duan L., Huang K., Tai H., Wang J., Poon L.L.M., Peiris J.S.M., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China , Journal of Virology. 2007, doi:10.1128/JVI.01157-07.

Cheung C.L., Fan X., Smith G.J., Zhang J., Chen H. and Guan Y., Establishment of influenza A virus (H6N1) in minor poultry in southern China, Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Dhanasekaran V., Zhang J., Smith G.J., Chen H. and Guan Y., Ecology and evolution of coronaviruses, The XIth International Nidoviral Symposium St Catherine's College, Oxford, UK, 2008.

Dong B.Q., Liu W., Fan X.H., Dhanasekaran V., Tang X., Gao F., Li L., Li G.J., Zhang J., Yang L.Q., Poon L.L.M., Zhang S.Y., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Detection of a novel and highly divergent coronavirus from Asian leopard cat and Chinese ferret badger in southern China, Journal of Virology. 2007, 81: 6920-6926.

Duan L., Campitelli L., Fan X.H., Leung C.Y.H., Dhanasekaran V., Zhang J., Donatelli I., Delogu M., Li K.S., Foni E., Chiapponi C., Wu W.L., Huang K., Webster R.G., Shortridge K.F., Peiris J.S.M., Smith G.J., Chen H. and Guan Y., Characterization of low pathogenic H5 subtype influenza viruses from Eurasia: Implications for the origin of highly pathogenic H5N1 viruses, Journal of Virology. 2007, 81: 7529-7539.

Qin K., Ma S.K., Smith G.J., Zhang J., Shortridge K.F., Chen H., Peiris J.S.M. and Guan Y., Characterization of H3N2 influenza viruses isolated from pigs in southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Rayner J.M., Cheung C.L., Smith G.J., Wang P., Tai H., Song W., Zhang J., Peiris J.S.M., Guan Y. and Chen H., Naturally occurring antiviral drug resistance in avian H5N1 virus , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesia and Vietnam avian influenza A (H5N1) viruses from southern China. , Journal of Virology. 2008, 82: 3405-3414.

Wang J., Dhanasekaran V., Duan L., Bahl J., Zhang J., Webster R.G., Peiris J.S.M., Chen H., Smith G.J. and Guan Y., Identification of the progenitors of Indonesian and Vietnamese avian influenza A (H5N1) viruses from southern China, Australian Society for Microbiology AGM, 2008, Melbourne, Australia. 2008.

Xu K., Zhang J., Duan L., Smith G.J., Peiris J.S.M., Chen H. and Guan Y., Evolution of H9N2 influenza A viruses in quail from southern China , Options for the Control of Influenza VI, 2007, Toronto, Canada. 2007.

Xu K., Smith G.J., Bahl J., Duan L., Tai H., Dhanasekaran V., Wang J., Zhang J., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S.M. and Guan Y., The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to 2005 , Journal of Virology. 2007, doi:10.1128/JVI.00979-07.

Zhang J., Dhanasekaran V., Liu W., Dong B., Zhu H., Li L., Smith G.J., Chen H. and Guan Y., Interspecies transmission of coronaviruses in wild animals, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Researcher : Zhang L

List of Research Outputs

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Effect of interventions on influenza a (H9N2) isolation in Hong Kong's live poultry markets, 1999-2005, Emerging Infectious Diseases. 2007, 13(9): 1340-1347.

Lau E.H.Y., Leung C.Y.H., Zhang L., Cowling B.J., Mak S.P., Guan Y., Leung G.M. and Peiris J.S.M., Minimizing risk of avian influenza: The effect of interventions on H9N2 influenza isolation in live poultry markets, 1999-2005 (abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 8 September 2007, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2007, Free Paper 5.

Leung C.Y.H., Zhang L., Chow C.K., Tsang C.L., Ng C.F., Wong C.K., Guan Y. and Peiris J.S.M., Poultry Drinking Water Used for Avian Influenza Surveillance., Emerging Infectious Disease. 2007, 13(9): 1380-1382.

Researcher : Zhang Q

List of Research Outputs

Zhang H., Wang Y.X., Wu H., Zhang Q., Wen Y.M. and Zheng B., Mutations of the turn between helix H and I of HIV-1 reverse transcriptase affect RT incorporation into viral particles and subsequent viral replication , 25 years of HIV.. 2008.

Researcher : Zhao G

List of Research Outputs

Du L., Kao R.Y.T., Zhou Y., He Y., Zhao G., Wong K.L., Jiang S., Yuen K.Y., Jin D. and Zheng B., Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity., Biochem. Biophys. Res. Commun.. 2007, 359(1): 174-179.

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Du L., Zhao G., Lin Y., Chan C.S., He Y., Jian S., Wu C.Y., Jin D., Yuen K.Y., Zhou Y. and Zheng B., Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection, Vaccine. 2008, 26(13): 1644-1651.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Researcher : Zheng B

Project Title:	The pattern of chemokine receptor polymorphism in healthy and HIV positive Chinese adults
Investigator(s):	Zheng B, Lee SS
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	01/2001

Abstract:

To determine the prevalence of the major forms of CCR5 promoter and CCR2 polymorphism in healthy Chinese adults; to compare the pattern between healthy adults and HIV infected individuals in Hong Kong.

Project Title:	CCR5 expression on antigen-presenting cens in HIV infection
Investigator(s):	Zheng B, Lee SS
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	01/2001

Abstract:

To describe the pattern of CCR5 expression on APC in HIV infection; to correlate this pattern to HIV infected APC; to determine the functional and/or quantitative deficiency in CCR5 expressing APC.

Project Title:	Studies of SARS virus vaccines
Investigator(s):	Zheng B, Guan Y
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2005

Abstract:

The study objectives are developments of an inactivated vaccine and a cold attenuated live vaccine.

Project Title:	A longitudinal study of HIV-1 drug-resistance for improving management of patients on HARRT (MSS 140R)
Investigator(s):	Zheng B, Lu L, Chan KW
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	07/2006

Abstract:

To define and confirm the specificities of viral gene mutations in non-B subtypes of HIV-1 that are responsible for drug resistance, identify potential drug-resistance before treatment failure, and analyze the circumstances for the causation of latent infection by drug-resistant viruses.

Project Title:	A feasibility study on the inhibition of avian infuenza virus H5N1 replication using siRNA
Investigator(s):	Zheng B, Yuen KY, Jin D
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2007

Abstract:

(1) Rational design and evalution of additional siRNAs targeting multiple structural and non-structural genes of H5N1 virus in cultured cells; (2) analysis of the synergistic antiviral effects of different siRNAs obtained; (3) development of effective non-viral and viral based systems for delivering H5N1-targeting siRNAs in mice and evaluation of the antiviral effects of selective siRNAs in vivo.

Project Title:	A novel anti-H5N1 avian influenza virus agent using silver nano-particle
Investigator(s):	Zheng B, Yuen KY, Che CM, Tam PKH, Huang J
Department:	Microbiology
Source(s) of Funding:	Guangdong-Hong Kong Technology Cooperation Funding Scheme
Start Date:	03/2007

Abstract:

To optimize size and conformation of silver nanoparticle to reach the hoghest effectiveness for anti-H5N1 viral infection in cell culutre system. To study underlying mechanism of silver nanoparticle in inhibiting H5N1 viral infection. To investigate the prophylactic and therapeutic effects of silver nanoparticle in the H5N1 viral infection in an animal model. To evaluate the safety of silver nanoparticle for prevention and treatment in animal models.

List of Research Outputs

Cao K., Mao X.P., Wang D.H., Xiao N., Xu L., Yuan G.Q., Dai S.Q., Zheng B. and Qiu S.P., High Expression of PSM-E Correlated with Tumor Grade in Prostate Cancer: A New Alternatively Spliced Variant of Prostate-Specific Membrane Antigen., Prostate. 2007, 67(16): 1791-1800.

Chen Y., Chan V.S.F., Zheng B., Chan Y.K., Xu X., To Y.F., Huang F.P., Khoo U.S. and Lin C.L., A novel subset of putative stem/progenitor CD34+Oct-4+ cells is the major target for SARS coronavirus in human lung., J Exp Med.. 2007, 204: 2529-36.

Du L., Kao R.Y.T., Zhou Y., He Y., Zhao G., Wong K.L., Jiang S., Yuen K.Y., Jin D. and Zheng B., Cleavage of spike protein of SARS coronavirus by protease factor Xa is associated with viral infectivity., Biochem. Biophys. Res. Commun.. 2007, 359(1): 174-179.

Du L., Jian S. and Zheng B., Development of subunit vaccines against severe acute respiratory symptom., Drugs of Today. 2008, 44(1): 63-73.

Du L., Zhao G., Lin Y., Sui H., Chan C.S., Ma S., He Y.X., Jian S., Wu C.Y., Yuen K.Y., Jin D., Zhou Y.S. and Zheng B., Intranasal Vaccination of Recombinant Adeno-Associated Virus Encoding Receptor-Binding Domain of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Spike Protein Induces Strong Mucosal Immune Responses and Provides Long-Term Protection against SARS-CoV Infection., Journal of Immunology. 2008, 180(2): 948-956.

Du L., Zhao G., Lin Y., Chan C.S., He Y., Jian S., Wu C.Y., Jin D., Yuen K.Y., Zhou Y. and Zheng B., Priming with rAAV encoding RBD of SARS-CoV S protein and boosting with RBD-specific peptides for T cell epitopes elevated humoral and cellular immune responses against SARS-CoV infection, Vaccine. 2008, 26(13): 1644-1651.

Lam Q.L.K., Zheng B., Jin D., Cao X. and Lu L., Leptin induces CD40 expression through the activation of AKT in murine dendritic cells, J Biol Chem. 2007, 38: 27587-27597.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Wang M., Lam S.F., Xu H., Guo R., Chan K.H., Zheng B. and Yuen K.Y., Complete genome sequence of bat coronavirus HKU2 from Chinese horseshoe bats revealed a much smaller spike gene with a different evolutionary lineage from the rest of the genome., Virology. 2007, 367(2): 428-439.

Lau S.K.P., Woo P.C.Y., Tse H., Fu C.T., Au W.K., Chen X.C., Tsoi H.W., Tsang T.H.F., Chan J.S.Y., Tsang D.N.C., Li K.S.M., Tse C.W.S., Ng T.K., Tsang O.T.Y., Zheng B., Tam S., Chan K.H., Zhou B. and Yuen K.Y., Identification of novel porcine and bovine parvoviruses closely related to human parvovirus 4, Journal of General Virology. 2008, 89: 1840-1848.

Li Z., Yao H., Ma Y., Dong Q., Peng Y., Zheng B., Huang J., Chan C.Y., Lin M.C., Sung J.J., Yuen K.Y., Kung H.F. and He M.L., Inhibition of HBV gene expression and replication by stably expressed interferon-α1 via adeno-associated viral vectors, J Gene Med.. 2008, 10(6): 619-627.

Ma N., Hu L., Fung J.M.W., Xie D., Zheng B., Chen L., Tang D., Fu L., Wu Z., Chen M., Fang Y. and Guan X.Y., Isolation and characterization of a novel oncogene, amplified in liver cancer 1, within a commonly amplified region at 1q21 in hepatocellular carcinoma., Hepatology. 2008, 47: 503-510.

Ma S.K.Y., Lee K.Y., Zheng B. and Chan K.W., CD133(+) HCC cancer stem cells confer chemoresistance by preferential expression of the Akt/PKB survival pathway., Oncogene. 2008, 27: 1749-1758.

Ma S.K.Y., Lee K.W., Zheng B., Chan K.W. and Guan X.Y., CD133+ HCC cancer stem cells promote chemoresistance by preferential expression of the Akt/PKB survival pathway, Oncogene. 2008, 27: 1749-1758.

Wang Y.X., Zhang H., Xu J., Wen Y.M. and Zheng B., Turn residue at the helix clamp motif of reverse transcriptase is crucial for replication for both hepatitis B virus and human immunodeficiency virus , 2007 International Meeting on the Molecular Biology of Hepatitis B Viruses. 2007.

Yang N., Tanner J.A., Zheng B., He M.L., Lu L., Jiang J.Q., Shum K.T., Lin Y., Wong K.L. and Lin M.C., Bismuth Complexes Inhibit the SARS Coronvirus, Angew. Chem. Int. Ed. 2007, 46: 6464.

Yang N., Tanner J.A., Zheng B., Watt R.M., He M.L., Lu L., Jiang J., Shum K.T., Lin Y., Wong K.L., Lin M.C., Kung H.F., Sun H. and Huang J., Bismuth complexes inhibit the SARS coronavirus, Angewandte Chemie International Edition. 2007, 46: 6464-6468.

Yang N., Tanner J.A., Wang Z., Huang J., Zheng B., Zhu N. and Sun H., Inhibition of SARS coronavirus helicase by bismuth complexes, Chemical Communications. 2007, 4413-4415.

Yang N., Tanner J.A., Huang J., Zheng B. and Sun H., Inhibition of the SARS coronavirus by Bismuth Compounds, 13^th International Conference on Biological Inorganic Chemistry (ICBIC-13), Vienna, Austria, July 15-20. 2007.

Zhang H., Wang Y.X., Wu H., Zhang Q., Wen Y.M. and Zheng B., Mutations of the turn between helix H and I of HIV-1 reverse transcriptase affect RT incorporation into viral particles and subsequent viral replication , 25 years of HIV.. 2008.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., A feasibility study for detection of HIV specific cytotoxic T lymphocytes using rAAV delivery system. , 4th IAS Conference on HIV Pathogenesis, Treatment and Prevention.. Sydney, Australia., 2007.

Zheng B., Chan K.W., Lin Y., Zhao G., Chan C.S., Zhang H., Chen H., Wong S.S.Y., Lau S.K.P., Woo P.C.Y., Chan K.H., Jin D. and Yuen K.Y., Delayed antiviral plus immunomodulator treatment still reduces mortality in mice infected by high inoculum of influenza A/H5N1 virus., Proc Natl Acad Sci USA.. 2008, 105(23): 8091-8096.

Zheng B., Editorial Board Member, The World Journal of Gastroenterology. 2007.

Zheng B., Yao X., Zhou J. and Wen Y.M., HBsAg-HBIg complex modulates antigen presentation of dendritic cells from chronic hepatitis B patients. , Hep DART 2007: frontiers in drug development for viral hepatitis.. 2007.

Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., A mechanism underlying susceptibility to HBV infection: HBV down-regulate transferase-1 type I interferon receptor 1 via suppressing poly(ADP-ribose), 2007 International Meeting: The Molecular Biology of Hepatitis B Viruses.. 2007.

Researcher : Zhou C

List of Research Outputs

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, International Journal of Infectious Diseases. 2008, 12: S45.

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, The 15th International Symposium on Infections in the Immunocompromised Host. 2008.

Researcher : Zhou C

List of Research Outputs

Zhou C., Tse H., Lau S.K.P., Woo P.C.Y. and Yuen K.Y., Genome Analysis Reveals Horizontal Gene Transfer in Penicillium marneffei, International Journal of Infectious Diseases. 2008, 12: S45.

Researcher : Zhou J

List of Research Outputs

Zheng B., Yao X., Zhou J. and Wen Y.M., HBsAg-HBIg complex modulates antigen presentation of dendritic cells from chronic hepatitis B patients. , Hep DART 2007: frontiers in drug development for viral hepatitis.. 2007.

Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., A mechanism underlying susceptibility to HBV infection: HBV down-regulate transferase-1 type I interferon receptor 1 via suppressing poly(ADP-ribose), 2007 International Meeting: The Molecular Biology of Hepatitis B Viruses.. 2007.

Researcher : Zhu H

List of Research Outputs

Zhang J., Dhanasekaran V., Liu W., Dong B., Zhu H., Li L., Smith G.J., Chen H. and Guan Y., Interspecies transmission of coronaviruses in wild animals, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

Zhu H., Li L., Tang X., Dhanasekaran V., Zhang J., Smith G.J., Zhang S., Chen H. and Guan Y., Coronaviruses detected from bats in China and Vietnam, The XIth International Nidovirus Symposium St Chatherine's, Oxford, UK, 2008.

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