HKU-PASTEUR RESEARCH CENTRE LIMITED

Researcher : Altmeyer RM

List of Research Outputs

 

Altmeyer R.M., Inhibitors of Virus-Cell Membrance Fusion, 2006.

 

Garcia J., Choi P.S., Altmeyer R.M., Li J., Nan F., Zuo J.P. and Schwartz O., Identification of Novel Anti-HIV Inhibitors by High-Throughput Screen of an Original Library of Pure Chemical Compounds, Fourth ICAV International and First Pacific Rim ICAV Meeting. 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FCGAMMARII-MEDIATED SARS-COV ENTRY IN HUMAN B CELL LINES, Scientific Conference of the Board of Directors of the International Network of Institut Pasteur (RIIP) (VIETNAM). 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E.W., Vogel L., Chu S.L., Tse K.S., Guarner J., Zaki S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Vaccine, Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Epub, 2007, 25(4): 729-40.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., H5 pseudotyped lentiviral particles: A New tool for sero-diagnosis of influenza H5N1 infection, IX International Symposium on Respiratory Viral Infections. 2007.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., Hemagglutinin pseudotyped lentiviral particles: Characterization of a new tool for avian influenza H5N1 influenza serodiagnostic, Journal of clinical Virology. 2007, 39(1): 27-33.

 

Researcher : Choi PS

List of Research Outputs

 

Garcia J., Choi P.S., Altmeyer R.M., Li J., Nan F., Zuo J.P. and Schwartz O., Identification of Novel Anti-HIV Inhibitors by High-Throughput Screen of an Original Library of Pure Chemical Compounds, Fourth ICAV International and First Pacific Rim ICAV Meeting. 2006.

 

Researcher : Chu SL

List of Research Outputs

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E.W., Vogel L., Chu S.L., Tse K.S., Guarner J., Zaki S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Vaccine, Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Epub, 2007, 25(4): 729-40.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., H5 pseudotyped lentiviral particles: A New tool for sero-diagnosis of influenza H5N1 infection, IX International Symposium on Respiratory Viral Infections. 2007.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., Hemagglutinin pseudotyped lentiviral particles: Characterization of a new tool for avian influenza H5N1 influenza serodiagnostic, Journal of clinical Virology. 2007, 39(1): 27-33.

 

Researcher : Garcia J

List of Research Outputs

 

Garcia J., Choi P.S., Altmeyer R.M., Li J., Nan F., Zuo J.P. and Schwartz O., Identification of Novel Anti-HIV Inhibitors by High-Throughput Screen of an Original Library of Pure Chemical Compounds, Fourth ICAV International and First Pacific Rim ICAV Meeting. 2006.

 

Garcia J., Young Investigator Award, IX International Symposium on Respiratory Viral Infections. 2007.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., H5 pseudotyped lentiviral particles: A New tool for sero-diagnosis of influenza H5N1 infection, IX International Symposium on Respiratory Viral Infections. 2007.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., Hemagglutinin pseudotyped lentiviral particles: Characterization of a new tool for avian influenza H5N1 influenza serodiagnostic, Journal of clinical Virology. 2007, 39(1): 27-33.

 

Researcher : Jaume MDA

List of Research Outputs

 

Jaume M.D.A., Antibody-dependent enhancement (ADE) and vaccination, 3rd Pasteur-Asia Virology Courses. 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FCGAMMARII-MEDIATED SARS-COV ENTRY IN HUMAN B CELL LINES, Scientific Conference of the Board of Directors of the International Network of Institut Pasteur (RIIP) (VIETNAM). 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Researcher : Kam YW

List of Research Outputs

 

Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FCGAMMARII-MEDIATED SARS-COV ENTRY IN HUMAN B CELL LINES, Scientific Conference of the Board of Directors of the International Network of Institut Pasteur (RIIP) (VIETNAM). 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E.W., Vogel L., Chu S.L., Tse K.S., Guarner J., Zaki S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Vaccine, Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Epub, 2007, 25(4): 729-40.

 

Researcher : Kien FS

List of Research Outputs

 

Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FCGAMMARII-MEDIATED SARS-COV ENTRY IN HUMAN B CELL LINES, Scientific Conference of the Board of Directors of the International Network of Institut Pasteur (RIIP) (VIETNAM). 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E.W., Vogel L., Chu S.L., Tse K.S., Guarner J., Zaki S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Vaccine, Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Epub, 2007, 25(4): 729-40.

 

Kien F.S., Millet J.K.G., Teoh K.T., Siu Y.L. and Nal-Rogier B.T.M., Identification Of Cellular Factors Which Regulate Early And/or Late Stages of Sars Cov Replication Cycle., Scientific conference of the 38th Board of Institutes Pasteur Directors, National Institute of Hygiene and Epidemiology. . 2006.

 

Nal-Rogier B.T.M., Kien F.S., Siu Y.L., Millet J.K.G., Teoh K.T. and Tse K.S., Tracking Virus-Host Cell Interactions: Example of the SARS coronavirus. , 1st RESPARI Workshop. Hong Kong . 2007.

 

Researcher : Lagarde NJC

List of Research Outputs

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., H5 pseudotyped lentiviral particles: A New tool for sero-diagnosis of influenza H5N1 infection, IX International Symposium on Respiratory Viral Infections. 2007.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., Hemagglutinin pseudotyped lentiviral particles: Characterization of a new tool for avian influenza H5N1 influenza serodiagnostic, Journal of clinical Virology. 2007, 39(1): 27-33.

 

Researcher : Li PH

List of Research Outputs

 

Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FCGAMMARII-MEDIATED SARS-COV ENTRY IN HUMAN B CELL LINES, Scientific Conference of the Board of Directors of the International Network of Institut Pasteur (RIIP) (VIETNAM). 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Researcher : Nal-Rogier BTM

List of Research Outputs

 

Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FCGAMMARII-MEDIATED SARS-COV ENTRY IN HUMAN B CELL LINES, Scientific Conference of the Board of Directors of the International Network of Institut Pasteur (RIIP) (VIETNAM). 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E.W., Vogel L., Chu S.L., Tse K.S., Guarner J., Zaki S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Vaccine, Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Epub, 2007, 25(4): 729-40.

 

Kien F.S., Millet J.K.G., Teoh K.T., Siu Y.L. and Nal-Rogier B.T.M., Identification Of Cellular Factors Which Regulate Early And/or Late Stages of Sars Cov Replication Cycle., Scientific conference of the 38th Board of Institutes Pasteur Directors, National Institute of Hygiene and Epidemiology. . 2006.

 

Nal-Rogier B.T.M., Large-scale Genomic Approaches To Identify Cellular Regulators Of Virus Life Cycle., Department of Virology. Pasteur Institute, Paris, France . 2006.

 

Nal-Rogier B.T.M., Kien F.S., Siu Y.L., Millet J.K.G., Teoh K.T. and Tse K.S., Tracking Virus-Host Cell Interactions: Example of the SARS coronavirus. , 1st RESPARI Workshop. Hong Kong . 2007.

 

Researcher : Nefkens I

List of Research Outputs

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., H5 pseudotyped lentiviral particles: A New tool for sero-diagnosis of influenza H5N1 infection, IX International Symposium on Respiratory Viral Infections. 2007.

 

Nefkens I., Garcia J., Lagarde N.J.C., Chu S.L., Peiris J.S.M., Buchy P. and Altmeyer R.M., Hemagglutinin pseudotyped lentiviral particles: Characterization of a new tool for avian influenza H5N1 influenza serodiagnostic, Journal of clinical Virology. 2007, 39(1): 27-33.

 

Researcher : Peiris JSM

Project Title:

The application of DNA arrays to characterise the avian influenza gene pool in southern China

Investigator(s):

Peiris JSM, Guan Y, Shortridge KF

Department:

Microbiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

12/2002

 

Abstract:

This project is a multi-institutional collaborative study for developing a high throughput DNA array to allow genotyping of influenza virus isolates of avian origin from Hong Kong and applying it to characterize the influenza virus gene pool in sourthern China. Through its application, it is expected to:- 1) identify the precursor virus that donated its internal genes to generate the mutiple reassortment of H5N1 isolated in Hong Kong markets in 2001; 2) define the frequency of reassortment between viruses of different virus lineages that co-circulate in poultry in Hong Kong and correlate this to host-specificity; and 3) investigate whether avian internal gene segments are transiently introduced into the human influenza gene pool.

 

Project Title:

Influenza pandemic preparedness in Asia (Research collaboration with national Institute of Allergy and Infectious Diseases (NIAID), St. Jude Children's Research Hospital, Inc. (SJCRH) and The University of Hong Kong)

Investigator(s):

Peiris JSM, Guan Y

Department:

Microbiology

Source(s) of Funding:

National Institutes of Health, US Department of Health and Human Services - General Award

Start Date:

04/2003

 

Abstract:

To study influenza pandemic preparedness in Asia.

 

Project Title:

Virus-cell interaction and the pathogenesis of SARS

Investigator(s):

Peiris JSM

Department:

Microbiology

Source(s) of Funding:

VCO SARS Research Fund

Start Date:

07/2003

 

Abstract:

To explore what is the biological basis for the selective attraction of macrophages to the lung; to study what immunopathological consequences follow.

 

Project Title:

Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?

Investigator(s):

Peiris JSM, Guan Y, Lau ASY, Poon LLM

Department:

Microbiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

09/2003

 

Abstract:

To identify the viral genetic determinants responsible for the differential cytokine hyper-induction associated with H5N1/97 viruses. We have already established the role of the viral NS gene, however, a role for other viral genes has not been excluded. we hypothesize (and have preliminary evidence) that one or more other viral genes are also involved in the hyper-induction of cytokines; to determine whether the avian viral genes/gene products of H5N1/97 directly up-regulate cytokine induction or lack "immune evasion" mechanisms evolved by human influenza viruses? to determine whether the differential up-regulation of cytokines associated with H5N1/97 is also observed in human lung epithelial cells (primary bronchial epithelial cells and human lung epithelial cell lines e.g. A549 cells.

 

Project Title:

The role of influenza virus gene constellation and viral morphology on cytokine induction, pathogenesis and viral virulence

Investigator(s):

Peiris JSM, Nicholls JM, Guan Y, Poon LLM

Department:

Microbiology

Source(s) of Funding:

Research Fund for the Control of Infectious Diseases - Full Grants

Start Date:

02/2005

 

Abstract:

To correlate the cytokine induction phenotype with viral genotype and morphology.

 

Project Title:

The macrophage in the pathogenesis of Severe Acute Respiratory Syndrome Coronavirus infection

Investigator(s):

Peiris JSM

Department:

Microbiology

Source(s) of Funding:

Research Fund for the Control of Infectious Diseases - Full Grants

Start Date:

12/2005

 

Abstract:

The global gene expression profiling of SARS-CoV infected macrophages will provide the basis for a more detailed analysis of the signaling pathways involved in subsequent studies, and may provide rational and novel therapeutic options to be devised for treating SARS.

 

Project Title:

Mass Tag PCR: A novel, multiplex and potentially sensitive approach for the rapid detection of respiratory pathogens

Investigator(s):

Peiris JSM, Chiu SSS, Cheung CY

Department:

Microbiology

Source(s) of Funding:

Research Fund for the Control of Infectious Diseases - Full Grants

Start Date:

06/2006

 

Abstract:

To divise, optimize and evaluate the Mass Tag PCR system for the rapid disgnosis for simultaneous screening of a wide spectrum of causative agents for human respiratory tract infections, including the avian influenza H5N1 and H7N7, SARS-coronavirus (SARS-CoV). Thiw will significantly facilitate better prevention, treatment and control of infectious diseases.

 

Project Title:

Pathogenesis, cell signaling and virus evolution of avian influenza A (H5N1)

Investigator(s):

Peiris JSM, Guan Y, Lau ASY, Nicholls JM, Chen H, Poon LLM, Lau YL, Yuen KY

Department:

Microbiology

Source(s) of Funding:

Central Allocation Vote - Group Research Project

Start Date:

06/2006

 

Abstract:

To study the continued evolution of H5N1 influenza viruses in Vietnam and Indonesia, in the context of the ongoing studies in Hong Kong and mainland China; to define differences in cell-signaling pathways activated by avian influenza A H5N1 compared to convetional human influenza (H1N1 or H3N2) in primary human macrophages and epithelial cells; to compare the biological characteristics of H5N1 viruses from Indonesia and Vietnam to define a basis for possible differential pathogenicity for humans; investigate the biological significance of specific viral genetic mutations/changes in recent h5N1 viruses in primary human cell culture models

 

Project Title:

Role of toll-like receptors and retinoic acid-inducible gene 1 in the induction of cytokines in SARS-coronavirus infected human primary macrophages

Investigator(s):

Peiris JSM

Department:

Microbiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

01/2007

 

Abstract:

To investigate the role of ToII-like receptors (TLR) and retinoic acid inducible gene (RIG-1) in the induction of pro-inflammatory cytokines, chemokines and interferon stimulated genes in SARS coronavirus and other coronavirus infected primary macrophages and dendritic of human and murine origin by using a) gene knock-down (siRNA); b) gene over-expression.

 

Project Title:

The pathogenesis of avian influenza A H5N1 in humans: macrophage and epithelial cell-virus interactions in vitro and ex-vivo

Investigator(s):

Peiris JSM

Department:

Microbiology

Source(s) of Funding:

The Wellcome Trust - General Award

Start Date:

01/2007

 

Abstract:

Specific aims are to: 1) Characterise the influenza viral replication profiles in tissue explant (ex-vivo) cultures of nasopharyngeal biopsies and lung tissue and primary cell cultures of human macrophages and respiratory (nasopharyngeal, bronchial, alveolar) epithelium. 2) Charaterise the functional receptors for H5N1 virus on macrophages and nasopharyngeal epithelium. 3) Define the consequences of viral entry via alternative pathways including the entry of non-neutralised virus antibody complexes via the Fc and complement receptors, to determine how these pathways affects subsequent viral replication and /or innate immune responses such as cytokine/chemokine secretion.

 

List of Research Outputs

 

Jaume M.D.A., Kien F.S., Kam Y.W., Li P.H., Cheung Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FCGAMMARII-MEDIATED SARS-COV ENTRY IN HUMAN B CELL LINES, Scientific Conference of the Board of Directors of the International Network of Institut Pasteur (RIIP) (VIETNAM). 2006.

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E.W., Vogel L., Chu S.L., Tse K.S., Guarner J., Zaki S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Vaccine, Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Epub, 2007, 25(4): 729-40.

 

Researcher : Siu YL

List of Research Outputs

 

Kien F.S., Millet J.K.G., Teoh K.T., Siu Y.L. and Nal-Rogier B.T.M., Identification Of Cellular Factors Which Regulate Early And/or Late Stages of Sars Cov Replication Cycle., Scientific conference of the 38th Board of Institutes Pasteur Directors, National Institute of Hygiene and Epidemiology. . 2006.

 

Nal-Rogier B.T.M., Kien F.S., Siu Y.L., Millet J.K.G., Teoh K.T. and Tse K.S., Tracking Virus-Host Cell Interactions: Example of the SARS coronavirus. , 1st RESPARI Workshop. Hong Kong . 2007.

 

Researcher : Tse KS

List of Research Outputs

 

Jaume M.D.A., Kien F.S., Kam Y.W., Corby P., Li P.H., Chu S.L., Tse K.S., CHEUNG Y.C., Peiris J.S.M., Daeron M., Nal-Rogier B.T.M. and Altmeyer R.M., FcgammaRII-mediated SARS-CoV entry in human B cell lines, 16th European Congress of Immunology - ECI (FRANCE). 2006.

 

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E.W., Vogel L., Chu S.L., Tse K.S., Guarner J., Zaki S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Vaccine, Antibodies against trimeric S glycoprotein protect hamsters against SARS-CoV challenge despite their capacity to mediate FcgammaRII-dependent entry into B cells in vitro. Epub, 2007, 25(4): 729-40.

 

Nal-Rogier B.T.M., Kien F.S., Siu Y.L., Millet J.K.G., Teoh K.T. and Tse K.S., Tracking Virus-Host Cell Interactions: Example of the SARS coronavirus. , 1st RESPARI Workshop. Hong Kong . 2007.



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