DEPT OF ANATOMY
Researcher
: Chan HC |
List of Research Outputs |
Chan H.C., Chang R.C.C., Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y. and So K.F., Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma, Experimental Neurology. 2006, 203: 269-273. |
Researcher
: Chan KC |
List of Research Outputs |
Chan K.C., Yau T.O., Ng I.O.L. and Chung S.K., Deleted in liver cancer 2
(DLC2) knockout mice show fast response to heat induced-pain, 11th
Postgraduate symposium 2006, |
List of Research Outputs |
Chan P.K., Philipsen S. and Tan-Un K.C., The study of sequence configuration and functional impact of the (AC)n(AT)xTy motif in human -globin gene promoter Am. J Hematol 82 (5) 342-8 (2007) , American Journal of Hematology . 2007, 82: 342-348. |
Researcher
: Chan SYM |
List of Research Outputs |
Fu Q., Wu W., Hu B., Chan S.Y.M., Shao Z., Pepinsky R.B., Mi S. and So K.F., LINGO-1 Antagonists protects retinal ganglion cells in a chronic hypertensive model of glaucoma, the 29th Annual Meeting of the Japan Neuroscience Society, Kyoto, Japan, July 19-21, 2006. S119 No. PS1P-E073. |
So K.F., Chan H.C., Chang R.C.C., Chan S.Y.M., Yuen W.H. and Zee S.S.Y., Modulation of microglia by Chinese herbal medicine Lycium barbarum and neuroprotection of retinal ganglion cells in experimental glaucoma, 4th Asian-Pacific International Congress of Anatomists, September 7-10, 2005, Kusadasi, Turkey. 2006, 37. |
Researcher
: Chan YF |
List of Research Outputs |
Chan Y.F., Adrenomedullin in the rat testis: Its production, functions and regulation in Sertoli cells and Leydig cells and its interaction with endothelin-1. 2006, 160 pages. |
Chan Y.F., O W.S. and Tang F., Testicular adrenomedullin and endothelin interaction, Society for Reproduction and Fertility Abstract Series No. 33 (ISSN 1476-3990). 2006, 33 No.P40. |
Researcher
: Chang RCC |
Project Title: |
Microglai/macrophages and neuroprotection in glaucoma |
Investigator(s): |
Chang RCC |
Department: |
Anatomy |
Source(s) of Funding: |
American Health Assistance Foundation - National Glaucoma Research |
Start Date: |
04/2005 |
Abstract: |
To prove or disapprove our hypothesis in a chronic neurodegenerative disease like glaucoma, we have set the following three specific aims for our study: (1) To investigate differential effects of conventional- or restricted-stimulation of microglia/ macrophages on RGC death in a chronic hypertension model of glaucoma induced by laser-photocoagulation. (2) To examine the expression of neurotrophic factors or pro-inflammatory factors from microglia/macrophages undergone conventional or restricted stimulation. (3) To study whether neuroprotective effects exhibited by Chinese herbal medicine Lycium barbarum (wolfberry) are mediated via restricted stimulation on microglia/ macrophages. |
Project Title: |
Elucidation of the functional roles of PKR in death receptor adaptors-mediated β-amyloid peptide neurotoxicity and in Alzheimer's disease |
Investigator(s): |
Chang RCC, So KF |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
11/2005 |
Abstract: |
To investigate how DR adaptor signaling modulates PKR in vitro so that inhibition of both pathways can maximize neuroprotection; to study the relationship of these two pathways and neuroprotection by inhibiting them in vivo; to examine the co-localization of accumulated phosphor-PKR and -DR adaptors in postmortem human AD brain tissues; to investigate whether activation of these two pathways can be found in plasma lymphocytes of AD patients. |
Project Title: |
Significant of double-stranded RNA-dependent protein kinase in neuronal death of Alzheimer's disease |
Investigator(s): |
Chang RCC |
Department: |
Anatomy |
Source(s) of Funding: |
France/Hong Kong Joint Research Scheme - Travel Grants |
Start Date: |
01/2006 |
Abstract: |
To investigate how death receptor adaptor signaling modulates PKR in vitro so that inhibition of both pathways can maximize neuroprotection; to examine the co-localization of accumlated phosphor-PKR and -DR adaptors in postmortem human AD brain tissues; to investigate whether activation of these two pathways can be found in plasma lymphocytes of AD patients. |
Project Title: |
Molecular signaling of synaptic degeneration in Alzheimer's disease |
Investigator(s): |
Chang RCC |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
02/2006 |
Abstract: |
Key issues and problems being addressed: The current treatment for Alzheimer’s disease (AD) is to preserve the cellular levels of neurotransmitter for memory. However, this strategy cannot prevent the loss of neurons or even be implemented in patients at early state of disease such as mild cognitive impairment (MCI). Alternative strategy to safeguard neurons such as prevention of neuronal loss or even loss of neuronal communications should be further explored. Increasing lines of evidence have shown that failure of inter-neuronal communication via synapses may contribute to early memory loss and precede neuronal degeneration. Accumulation of cerebral β-amyloid (Aβ) peptide attenuates induction of long-term potentiation (LTP) which is essential in leaning and memory. Loss of synapses (synaptic degeneration) is a structural change observed in the failure of inter-neuronal communication and memory. Therefore, synaptic degeneration is a key issue in studying dementia and cognitive decline in AD. Although amyloid plaque is a pathological feature of AD, many reports have demonstrated that plaques may not be the primary causes of synaptic degeneration and cognitive decline. In an experimental model of transgenic mice over-expressing human amyloid precursor protein (hAPP) to increase cerebral Aβ levels, synaptic deficits can be detected well before plaque formation. All these results support our hypothesis that synaptic degeneration is an early event leading to the obstruction of axonal transport and in turn neuronal apotposis. Increasing lines of evidence have demonstrated that oligomeric form of Aβ peptide can bind to α7 nicotinic receptor (α7nAchR) resulting in synaptic degeneration in cholinergic and glutamatergic neurons. Instead of fibrillary form of Aβ peptide found in the plaques, oligomeric form of Aβ peptide primarily binds to synapses. While evidence of synaptic degeneration has been documented, little is known about the signaling mechanisms of synaptic degeneration leading to other modes of degeneration such as blockage of axonal transport, formation of autophagic vesicles and neuronal apoptosis. It should be noted that blockage of axonal transport and neuronal apoptosis have long been demonstrated by Aβ peptide neurotoxicity. Therefore, the problem being addressed in this proposal is to investigate the molecular mechanisms from synaptic degeneration leading to blockage of axonal transport and neuronal apoptosis. Goal and specific aims: The goal of this proposed study is to elucidate molecular signaling events linking synaptic degeneration, blockage of axonal transport, formation of autophagic vesicles, and neuronal apoptosis. We will focus our study in the degeneration of synaptic terminals because concrete evidence has been made from our preliminary study about the loss of synaptic density proteins. To achieve this goal, we have three specific aims in this proposed study. (1) To trace the temporal profile of synaptic degeneration, axonal blockage, formation of autophagic vesicles and neuronal apoptosis in live cultured hippocampal neurons exposed to oligomeric Aβ peptide by using molecular biology and 2-photon confocal imaging techniques. (2) To investigate signaling events associated with the loss of glutamate receptors such as NMDA and AMPA receptors, mGluR5 and their scaffold proteins leading to formation of autophagic vesicles and neuronal apoptosis by using immunoprecipitation and western-blot analysis. (3) To elucidate the signaling events of translational control in synaptic degeneration and neuronal apoptosis because translational control has been implicated to play significant roles in synaptic plasticity, autophagic neuronal death and neuronal apoptosis. All these three specific aims can be worked out independently, but the implication of the results are highly connected. The results of this study can shed light of how different modes of neurodegeneration orchestrate together attributing to progressive neurodegeneration in AD. |
Project Title: |
Elucidating the biological mechanisms of autophagy in Alzheimer's disease |
Investigator(s): |
Chang RCC, |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2007 |
Abstract: |
To investigate how DR adaptor signaling modulates PKR in vitro so that inhibition of both pathways can maximize neuroprotection; to study the relationship of these two pathways and neuroprotection by inhibiting them in vivo; to examine the co-localization of accumulated phospho-PKR and -DR adaptors in postmortem human AD brain tissues; to investigate whether activation these two pathways can be found in plasma lymphocytes of AD patients. |
Project Title: |
Investigating the molecular events leading to the collapse of endoplasmic reticulum in neurodegeneration of Alzheimer's disease |
Investigator(s): |
Chang RCC |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
03/2007 |
Abstract: |
The objective of this proposed study is to elucidate the biological mechanisms and molecular events leading to collapse of the endoplasmic reticulum (ER) by β-amyloid (Aβ) peptide neurotoxicity in Alzheimer's disease (AD). To address our objective, I have set the following three specific aims in this study:1. To investigate how and to what extent these three factors: (a) distortion of intracellular cytoskeleton; (b) activation of calpain; and (c) activation of protease leads to collapse of ER in Aβ-peptide neurotoxicity.2. To examine whether Rho-associated coiled-coil-containing protein kinase (ROCK) modulate cytoskeleton resulting in ER collapse in Aβ-peptide neurotoxicity.3. To test whether tau protein-phosphorylation kinases (glycogen synthase kinase 3β, GSK3β; cyclin-dependent protein kinase 5, CDK5) modulate cytoskeleton resulting in ER collapse in Aβ-peptide neurotoxicity. Degeneration of neurons is a long-term key issue in AD. Neurons can undergo neuronal apoptosis, autophagic death, synaptic degeneration and dysfunction of axonal transport in the pathological processes of AD. While these four modes of degenerative processes have long been studied, my laboratory has recently discovered a new mode of death process, namely collapse of ER (Lai et al., In preparation). Using live-cell imaging technology, cultured neurons exposed to Aβ peptide (the toxin in AD) displays collapse of ER shortly right after exposure. The collapsed ER gradually aggregates in the cell body and are digested by lysosomes. In addition, aggregated ER fragment triggers the formation of autophagosomes, initiating the processes of autophagy. In view of all these new findings, the urgent key issue is to investigate the molecular events and even signaling pathways leading to the collapse of ER in Aβ-peptide neurotoxicity. Collapse of ER may explain why extracellular Aβ peptide could not trigger ER-stress responses signaling. The ER-stress responses are potentially beneficial to neurons as they can stop the accumulation of mis-folded proteins in the ER. While Aβ peptide induces ER collapse and does not trigger ER-stress responses, similar responses cannot be observed in parkinsonism mimetics such as 6-hydroxydopamine and MPP+. Therefore, ER collapse may represent a new mode of death process uniquely found in Aβ peptide neurotoxicity of AD. The scientific merit of this proposed study is to elucidate the biological mechanisms of this novel mode of neurodegeneration. Since all the new discoveries are originated from this laboratory, the study has very high originality. |
List of Research Outputs |
Chan H.C., Chang R.C.C., Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y. and So K.F., Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma, Experimental Neurology. 2006, 203: 269-273. |
Chang R.C.C., Lai S.W. and Yu M.S., Collapse of endoplasmic reticulum as a new mechanism mediating beta-amyloid peptide-triggered neurotoxicity, Alzheimer's and Parkinson's Diseases: Progress and New Perspectives, 8th International Conference AD/PD, Salzburg, Austria, March 14-18, 2007. 4(suppl 1): 61-62. |
Chang
R.C.C., Molecular Signaling Of Protein
Translation Control In Neuronal Apoptosis In Alzheimer's Disease, Institute
for Surgical Research, |
Chang R.C.C., Roles of Protein translation control in neurodegeneration of Alzheimer's disease, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 2006. |
Chang R.C.C., Suen A.K.C., Yu M.S., Lai S.W., Cheung Y.T. and Hugon J., Roles of protein translation control in neurodegeneration of Alzheimer's Disease, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientifiic Meeting of The Hong Kong society of Neurosciences, Nov. 30 - Dec. 2, 2006. 7 No. S6. |
Chang R.C.C., Yu M.S. and Lai S.W., Significance of molecular signaling for protein translation control in neurodegenerative diseases, Neurosignals. 2007, 15: 249-258. |
Chang R.C.C., The impact from understanding the biological mechanisms of neurodegeneration in Alzheimer's disease to the development of neuroprotective agents, 2007 World Congress on Ageing and Dementia in Chinese Communities, Hong Kong.. 2007. |
Chang R.C.C., Lai S.W., Yu M.S., Cheung Y.T. and Ho Y.S., The impact from understanding the biological mechanisms of neurodegeneration in Alzheimer's disease to the development of neuroprotective agents, Asian Journal of Gerontology & Geriatrics. 2007, 2(1): 31. |
Chiu K., Ji J., Yu M.S., So K.F. and Chang R.C.C., Activation of microglia/macrophages determines the fate of retinal ganglion cell survival in rat chronic ocular hypertension model, Neurosignals. 2006, 15: 139. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Alkaline extract of lycium barbarum protects against beta-amyloid peptide neurotoxicity in rat cortical neurons by activation of AKT, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 126-127 No. P-C36. |
Ho
Y.S., Yu M.S., So K.F., Yuen W.H. and Chang R.C.C., Attenuation of
unfolded protein responses by reducing stress: an example of neuroprotective
effect of Lycium barbarum, 2006 Hong Kong-Macau Postgraduate Symposium
on Chinese Medicine, August 17, 2006, |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Characterizing the Neuroprotective Effects of Alkaline Extract of Lycium Barbarumon b-amyloid Peptide Neurotoxicity , Brain Research . 2007, 1158: 123-134. |
Ho Y.S., Yu M.S., Lai S.W., So K.F. and Chang R.C.C., Glycoconjugates from anti-aging Lycium Barbarum protect primary cortical neurons from beta-amyloid neurotoxicity, Second International Symposium on Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4, 2007 Hong Kong. 2007, 53 P2. |
Ho Y.S., Yu M.S., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity, Society for Neuroscience. 2006, Program No. 826.10. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Neuroprotective effects of anti-aging Lycium barbarum by a novel extraction method, 2006 World Congress on Chinese Medicine: Charting the Course of Development, Hong Kong, November 23-25, 2006. 247. |
Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y., Chang R.C.C. and So K.F., Neuroprotective effect of Lycium barbarum in rat chronic ocular hypertension model via immunomodulation of macrophages/microglia, Neurosignals. 2006, 15: 145. |
Lai S.W., So K.F. and Chang R.C.C., Aggregation/collapse of endoplasmic retiulum induced by Ab subsequently undergo autophagy, Second International Symposium on Healthy Aging; Meeting the Challenges of an Aging Population, March 3-4, 2007, Hong Kong. 2007, 53 P4. |
Lai S.W., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Neuroprotective effects of the gandoderma lucidum aqueous extract against beta-amyloid peptide-induced neurotoxicity, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006 . 2006, 97 No. P-B36. |
Lai S.W., Yu M.S., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Potential neuroprotective agent from botanical extract: An experience of using Verbena officinalisagainst b-amyloid peptide neurotoxicity, Neurosignals. 2006, 15: 146. |
Lai S.W., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., The aqueous extract from anti-aging Ganoderma lucidum inhibits beta-amyloid peptide-induced neurotoxicity, Society for Neuroscience. 2006, Program No. 826.11. |
Lau K.W., Lai C.S., Yuen W.H., So K.F. and Chang R.C.C., Differential effects of parkinsonism mimetics on SH-SY5Y neuroblastoma , Society for Neuroscience. 2006, Program No. 824.19. |
Lau K.W., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma. Does it differentiate them?, The 4th Congress of the Federation of Asian-Oceanian Neuroscience societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 97-98 No. P-B37. |
Lau
K.W., Lai S.W., So K.F. and Chang R.C.C., Impact of retinoic
acid on SH-SY5Y neuroblastoma to neurotoxins, Second International
Symposiunm on Healthy Aging: Meeting the Challenges of an Aging Population,
March 3-4, 2007, |
So K.F., Chan H.C., Chang R.C.C., Chan S.Y.M., Yuen W.H. and Zee S.S.Y., Modulation of microglia by Chinese herbal medicine Lycium barbarum and neuroprotection of retinal ganglion cells in experimental glaucoma, 4th Asian-Pacific International Congress of Anatomists, September 7-10, 2005, Kusadasi, Turkey. 2006, 37. |
So
K.F. and Chang R.C.C.,
Molecular basis of neuroprotection in glaucoma and Alzheimer's disease using
Gouqizi, 2006 World Congress on Chinese Medicine: Charting the Course of
Development, November 23-26, 2006, |
So K.F. and Chang R.C.C., Molecular basis of neuroprotection using anti-ageing chinese herb, 6th International Symposium on Frontiers in Life Sciences, Qingdao, China, September 20-23, 2006. |
Suen K., Ho H., So C., Ma K. and Chang R.C.C., Doing physical exercise has no indication on learning and memory enhancement in children aged 13-15., Society for Neuroscience. 2006, Program No. 365.4. |
Suen
K.C. and Chang R.C.C.,
Increment of sleeping time does not implicate enhancement on memory and
learning in adolescents (Neuroscientist - Teacher Partner Award), Society
for |
Sum M., Liu B., Sy L., Chan W., Wong C., Suen K. and Chang R.C.C., Increment of sleeping time does not implicate enhancement on memory and learning in adolescents, Society for Neuroscience. 2006, Program No. 365.3. |
Yeung
S.C., Chiu K., So K.F. and Chang R.C.C., The effects of
intravitreal injection of IL-10 on the survival of retinal ganglion cells in
the rat glaucoma model, The 5th Asia-Pacific symposium on Neural
Regeneration, |
Yik
S.Y., Ho Y.S., Lai S.W., So K.F. and Chang R.C.C., Significance of dsRNA
produced by viral infection in neurodegeneration, Second International
Symposium on Healthy Aging: Meeting the Challenges of an Aging Population,
March 3-4, 2007, |
Yu
M.S., So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., A new polysaccharide
from nerium indicum elicits neuroprotection against beta-amyloid peptides-induced
apoptosis, Second International Symposium on Healthy Aging: Meeting the
challenges of an Aging Population, March 3-4, 2007, |
Yu M.S., Lai S.W., Suen K.A., So K.F., Hugon J. and Chang R.C.C., Absence of unfolded protein responses in extracellular beta-amyloid peptide-induced neuronal apoptosis, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 129 No. P-C41. |
Yu M.S., Ho Y.S., So K.F., Yuen W.H. and Chang R.C.C., Cytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulum, Neurosignals. 2006, 15: 145. |
Yu M.S., Lai S.W., Suen K.C., Kwok N.S., So K.F., Hugon J. and Chang R.C.C., Extracellular accumulation of beta-amyloid peptides induces apoptosis in cultured neurons via a mechanism independent of unfolded protein responses, Neurosignals. 2006, 15: 141. |
Yu M.S., Wong Y.Y., So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., New polysaccharide from Nerium indicum protects neurons via stress kinase signaling pathway, Brain Research. 2007, 1153: 221-230. |
Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Protein kinases as technological platforms to screen neuroprotective agents from chinese medicine, Neurosignals. 2006, 15: 133. |
Yu M.S., Yuen W.H., So K.F. and Chang R.C.C., Significance of neuroprotective polysaccharide from the flowers of Nerium indicum in beta-amyloid peptides neurotoxicity, Society for Neuroscience. 2006, Program No. 826.9. |
Researcher
: Chen AYS |
List of Research Outputs |
Ho C.M.E., Lam K.S.L., Chen A.Y.S., Yip C.W., Arvindakshan M., Yamagishi S., Oates P.J., Ellery C.A., Chung S.S.M. and Chung S.K., Aldose reductase-deficient mice are protected from delayed motor nerve conduction velocity, increased c-Jun NH2-terminal kinase activation, depletion of reduced glutathione, increased superoxide accumulation, and DNA damage, Diabetes. 2006, 55(7): 1946-53. |
Researcher
: Chen B |
List of Research Outputs |
Chen B., So K.F., Yu E. and Tay D.K.C., Expression of nicotinamide adenine dinucleotide phosphate-diaphorase in the retina of postnatal golden hamsters deprived of light stimulation, Neuroscience Letters. 2006, 405: 74-78. |
Chen B., Suprachiasmatic nucleus projecting retinal ganglion cells in golden hamsters: development, morphology and relationship with NOS expressing amacrine cells, 2006, 134 pages. |
Li S.Y., Chen B., Tay D.K.C., Chan H.H.L., Pu M.L. and So K.F., Melanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension model, Investigative Ophthalmology & Visual Science. 2006, 47(7): 2951-2958. |
Pu M., Chen B., Li S.Y., Tay D.K.C. and So K.F., A suprachiasmatic nucleus projecting retinal ganglion cell exhibits an unusually large dendritic field in the hamster, NeuroReport. 2006, 17(14): 1469-1472. |
Researcher
: Cheung A |
Project Title: |
Telomere erosion and initiation of chromosomal instability in human cells undergoing immortalization |
Investigator(s): |
Cheung A, Tsao GSW, Guan XY |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2003 |
Abstract: |
To obtain telomere length profiles for indivdual chromosomes and to characterize dynamic telomere shortening in pre-crisis cells; to determine if the chromosomes with shorter telomeres or faster telomere shortening are more frequently involved in the formation of chromosome aberrations during immortalization. |
Project Title: |
Centromeric instability in human cells undergoing immortalization |
Investigator(s): |
Cheung A, Tsao GSW |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
03/2005 |
Abstract: |
The main objectives of this project are to: 1) investigate whether centromeric instability (characterized by dynamic formation of centromeric rearrangements, breaks, deletions or iso-chromosomes) is a general phenomenon in human cells undergoing immortalization; 2) examine whether DNA damage signals are colocalized with centromeres, and whether centromeric instability is associated with up-regulation of proteins promoting G2-M-phase transition, which is the checkpoint asking whether all DNA has been replicated and errors corrected. |
Project Title: |
Role of Id |
Investigator(s): |
Cheung A, Tsao GSW, Wong YC, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
01/2006 |
Abstract: |
Gain and amplification of chromosome 20q
is frequently observed in a wide variety of cancers, including esophageal
cancer. This chromosomal region contains a number of candidiate oncogenes
including AIB1, Id-1, MDM2, AURKA and ZNF217. The function and significance
of these genes in esophageal carcinogenesis remains elusive. Our recent
investigations on prostate and nasopharyngeal carcinomas have provided strong
evidence to support an oncogenic role of Id-1gene in epithelial cancers. We
hypothesize that Id-1 also functions as an oncogene in esophageal cancer and
that its oncogenic effect may be regulated through different pathways. This
project aims to investigate the role and mechanisms of action of Id |
Project Title: |
Id-1 protects esophageal cancer cells from apoptosis through activation of PI3K-Akt and NFκB signaling pathways |
Investigator(s): |
Cheung A |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2006 |
Abstract: |
Id-1 (inhibitor of differentiation or
inhibition of DNA binding) is a helix-loop-helix (HLH) protein which lacks
the basic domain for DNA binding and thus functions as a dominant inhibitor
of the basic HLH transcription factors by forming heterodimers, thus
inhibiting gene expression [Benezra et al. 1990]. Up-regulation of Id-1 has
been found in many types of human cancer including esophageal squamous cell
carcinoma (ESCC) [Hu et al. 2001]. In our recent paper, we reported that
ectopic expression of Id-1 stimulates esophageal cancer cell proliferation by
activation of MDM2 and suppression of p21 [Hui et al. 2006], rather than
activation of p16/Rb pathway as in prostate and hepatocellular cancer cells.
Our findings suggest that the oncogenic function of Id |
Project Title: |
Centromeric instability in human cells undergoing immortalization: implication for progression of chromosomal instability in carcinogenesis |
Investigator(s): |
Cheung A, Tsao GSW, Guan XY, Deng W |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2007 |
Abstract: |
To investigate whether centromeric instability is a general phenomenon in human cells undergoing immortalization; to study the mechanisms underlying centromeric instability in human cells undergoing immortalization. |
List of Research Outputs |
Cheung A., Editorial Board, In: Clive R Taylor, Jiang Gu, Applied Immunohistochemistry & Molecular Morphology. Lippincott Williams & Wilkins, 2007. |
Cheung A., Cheung P.Y., Deng W. and Tsao G.S.W., Immortalization of human esophageal epithelial cells by human telomerase reverse transcriptase (hTERT), Proceedings o the American Association for Cancer Research Annual Meeting, April 14-18, Los Angeles, U. S. A.. 2007, No. 4268. |
Fung K.L., Cheung H.W., Wong H.L., Yuen H.F., Ling M.T., Chan K.W., Wong Y.C., Cheung A. and Wang X., MAD2 expression and its significance in mitotic checkpoint control in testicular germ cell tumour. , Biochimica et Biophysica Acta - Molecular Cell Research . 2007, 1773: 821-832. |
Fung K.L., Cheung H.W., Ling M.T., Cheung A., Wong Y.C. and Wang X., Role of MEK/ERK pathway in the MAD2-mediated cisplatin sensitivity in testicular germ cell tumour cells, British Journal of Cancer. 2006, 95: 475-484. |
Li B., Cheung P.Y., Wang X., Tsao G.S.W., Ling M.T., Wong Y.C. and Cheung A., Id-1 protects esophageal cancer cells from TNF-a-induced apoptosis through activation of P13K/AKT/NFkb signaling pathway, Proceedings of the Aerican Association for Cancer Research Annual Meeting, Los Angeles, U. S. A., April 14-18, 2007.. 2007, No. 5162. |
Researcher
: Cheung AKH |
List of Research Outputs |
Ho H.T.B., Ko C.B., Cheung A.K.H., Lam A.K.M., Tam S., Chung S.K. and Chung S.S.M., Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice, Kidney International. 2007, 72: 63-71. |
Researcher
: Cheung ALM |
Project Title: |
Telomere erosion and initiation of chromosomal instability in human cells undergoing immortalization |
Investigator(s): |
Cheung A, Tsao GSW, Guan XY |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2003 |
Abstract: |
To obtain telomere length profiles for indivdual chromosomes and to characterize dynamic telomere shortening in pre-crisis cells; to determine if the chromosomes with shorter telomeres or faster telomere shortening are more frequently involved in the formation of chromosome aberrations during immortalization. |
Project Title: |
Centromeric instability in human cells undergoing immortalization |
Investigator(s): |
Cheung A, Tsao GSW |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
03/2005 |
Abstract: |
The main objectives of this project are to: 1) investigate whether centromeric instability (characterized by dynamic formation of centromeric rearrangements, breaks, deletions or iso-chromosomes) is a general phenomenon in human cells undergoing immortalization; 2) examine whether DNA damage signals are colocalized with centromeres, and whether centromeric instability is associated with up-regulation of proteins promoting G2-M-phase transition, which is the checkpoint asking whether all DNA has been replicated and errors corrected. |
Project Title: |
Role of Id |
Investigator(s): |
Cheung A, Tsao GSW, Wong YC, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
01/2006 |
Abstract: |
Gain and amplification of chromosome 20q
is frequently observed in a wide variety of cancers, including esophageal
cancer. This chromosomal region contains a number of candidiate oncogenes
including AIB1, Id-1, MDM2, AURKA and ZNF217. The function and significance
of these genes in esophageal carcinogenesis remains elusive. Our recent
investigations on prostate and nasopharyngeal carcinomas have provided strong
evidence to support an oncogenic role of Id-1gene in epithelial cancers. We
hypothesize that Id-1 also functions as an oncogene in esophageal cancer and
that its oncogenic effect may be regulated through different pathways. This
project aims to investigate the role and mechanisms of action of Id |
Project Title: |
Id-1 protects esophageal cancer cells from apoptosis through activation of PI3K-Akt and NFκB signaling pathways |
Investigator(s): |
Cheung A |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2006 |
Abstract: |
Id-1 (inhibitor of differentiation or
inhibition of DNA binding) is a helix-loop-helix (HLH) protein which lacks
the basic domain for DNA binding and thus functions as a dominant inhibitor
of the basic HLH transcription factors by forming heterodimers, thus
inhibiting gene expression [Benezra et al. 1990]. Up-regulation of Id-1 has
been found in many types of human cancer including esophageal squamous cell
carcinoma (ESCC) [Hu et al. 2001]. In our recent paper, we reported that
ectopic expression of Id-1 stimulates esophageal cancer cell proliferation by
activation of MDM2 and suppression of p21 [Hui et al. 2006], rather than
activation of p16/Rb pathway as in prostate and hepatocellular cancer cells.
Our findings suggest that the oncogenic function of Id |
Project Title: |
Centromeric instability in human cells undergoing immortalization: implication for progression of chromosomal instability in carcinogenesis |
Investigator(s): |
Cheung A, Tsao GSW, Guan XY, Deng W |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2007 |
Abstract: |
To investigate whether centromeric instability is a general phenomenon in human cells undergoing immortalization; to study the mechanisms underlying centromeric instability in human cells undergoing immortalization. |
Researcher
: Cheung HW |
List of Research Outputs |
Cheung H.W., Significance of mitotic checkpoint regulatory proteins in chemosensitivity of nasopharyngeal carcinoma cells, 2006, 218 pages. |
Chu
Q., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Garlic derivatives inhibit
prostate cancer cell growth and invasion through restoration of E-cadherin
expression, 8th Asian Congress of Urology, |
Fung K.L., Cheung H.W., Wong H.L., Yuen H.F., Ling M.T., Chan K.W., Wong Y.C., Cheung A. and Wang X., MAD2 expression and its significance in mitotic checkpoint control in testicular germ cell tumour. , Biochimica et Biophysica Acta - Molecular Cell Research . 2007, 1773: 821-832. |
Fung K.L., Cheung H.W., Ling M.T., Cheung A., Wong Y.C. and Wang X., Role of MEK/ERK pathway in the MAD2-mediated cisplatin sensitivity in testicular germ cell tumour cells, British Journal of Cancer. 2006, 95: 475-484. |
Howard E.W., Chua C.W., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Potent suppression of distant metastasis by garlic compound S-allylmercaptocysteine in an in vivo androgen independent prostate cancer model, AACR Special Conference in Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006, San Francisco, U. S. A.. 2006, A1. |
Researcher
: Cheung KHA |
List of Research Outputs |
Cheung K.H.A., Lo A.C.Y., Chung S.S.M. and Chung S.K., Deletion of aldose reductase
gene protects against neuronal death and edema in retina after transient
ischemia by preventing oxidative stress, 11th Research Postgraduate
Symposium 2006, |
Lo A.C.Y., Hung K.L., Cheung K.H.A., He Q., Chiu J., Chung S.S.M. and Chung S.K., Aldose reductase-deficient mice are protected from iron- and transferrin-related oxidative stress and cerebral ischemic injury, 36th Annual Meeting of the Society for Neuroscience 2006. |
Researcher
: Cheung MPL |
List of Research Outputs |
Liao S., Chow P.H., Cheung M.P.L. and O W.S., Leptin in embryos sired by males without accessory sex glands, Society for Reproduction and Fertility Abstract Series No. 33 (ISSN 1476-3990). 2006, 33 No. O16. |
Researcher
: Cheung PY |
List of Research Outputs |
Cheung A., Cheung P.Y., Deng W. and Tsao G.S.W., Immortalization of human esophageal epithelial cells by human telomerase reverse transcriptase (hTERT), Proceedings o the American Association for Cancer Research Annual Meeting, April 14-18, Los Angeles, U. S. A.. 2007, No. 4268. |
Li B., Cheung P.Y., Wang X., Tsao G.S.W., Ling M.T., Wong Y.C. and Cheung A., Id-1 protects esophageal cancer cells from TNF-a-induced apoptosis through activation of P13K/AKT/NFkb signaling pathway, Proceedings of the Aerican Association for Cancer Research Annual Meeting, Los Angeles, U. S. A., April 14-18, 2007.. 2007, No. 5162. |
Researcher
: Cheung SF |
List of Research Outputs |
Cheung S.F., Effects of endothelial cell-specific over-expression of endothelin-1 on diabetic and ischemic retinopathy. 2006, 138 pages. |
Researcher
: Cheung YT |
List of Research Outputs |
Chang R.C.C., Suen A.K.C., Yu M.S., Lai S.W., Cheung Y.T. and Hugon J., Roles of protein translation control in neurodegeneration of Alzheimer's Disease, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientifiic Meeting of The Hong Kong society of Neurosciences, Nov. 30 - Dec. 2, 2006. 7 No. S6. |
Chang R.C.C., Lai S.W., Yu M.S., Cheung Y.T. and Ho Y.S., The impact from understanding the biological mechanisms of neurodegeneration in Alzheimer's disease to the development of neuroprotective agents, Asian Journal of Gerontology & Geriatrics. 2007, 2(1): 31. |
Ng S.M., Cheung Y.T., An X.M., Chen Y.C., Li M., Li H.Y., Cheung K.C., Sze J., Lai L., Peng Y., Xia H.H.X., Wong B.C.Y., Leung S.Y., Xie D., He M.L., Kung H.F. and Lin M.C., Cell Cycle-related Kianse: A Novel Candidate Oncogene in Human Glioblastoma, Journal of the National Cancer Institute. 2007, 99(12): 936-948. |
Yiu S.M., Wong P., Lam T.W., Mui Y.C., Kung H.F., Lin M.C. and Cheung Y.T., Research Output Prize, Faculty of Engineering, The University of Hong Kong. 2006. |
Researcher
: Ching YP |
Project Title: |
Roles and regulation of group II p21-activated protein kinases:-implications in cancer metastasis |
Investigator(s): |
Ching YP, Jin D, Ng IOL |
Department: |
Pathology |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2005 |
Abstract: |
To study: (1) Characterisation of the
interaction between Pak5 and NM23 i) co-immunoprecipitation of Pak5 adn NM23
ii) defining the binding domain between Pak 5 and NM23 iii) xploring the
interaction between Pak4 adn NM23. (2) Impact of Pak5-NM23 interaction in the
biochemical properties of Pak5 and NM23 i) nucleotide diphosphate kinase
activity ii) GTPase activating activity iii) in vitro kinase activity iv)
subcellular localisation. 3) Roles of PakII in cancer metastasis using HCC as
a model i) expression profile of Pak |
Project Title: |
Roles of
p21-activated protein kinase (Pak) |
Investigator(s): |
Ching YP |
Department: |
Pathology |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
07/2005 |
Abstract: |
To characterize the overexpression of
Pak1 protein and its activities in human HCC; to delineate the signaling
pathways mediated by Pak |
Project Title: |
Roles of
p21-activated protein kinase (Pak) |
Investigator(s): |
Ching YP, Ng IOL, Jin D, Yau TO |
Department: |
Pathology |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2006 |
Abstract: |
(1) To characterize the mechanisms
leading to Pak1 overexpression in human HCC; (2) to delineate the roles of
Pak |
Project Title: |
Functional characterization of a putative tumour suppressor, AMP-activated protein kinase, in liver cancer |
Investigator(s): |
Ching YP |
Department: |
Pathology |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
02/2006 |
Abstract: |
Purpose of study Liver cancer
(hepatocellular carcinoma, HCC) is one of the most common cancers in the
world, especially in Asia and Africa, and is the third most common fatal
cancer in |
Project Title: |
Molecular
neurobiology: Regulation of p21-activated protein kinase |
Investigator(s): |
Ching YP |
Department: |
Anatomy |
Source(s) of Funding: |
Matching Fund for NSFC Young Researcher Award |
Start Date: |
01/2007 |
Completion Date: |
12/2009 |
Abstract: |
To study molecular neurobiology:
regulation of p21-activated protein kinase |
Project Title: |
Functional characterisation of a putative tumor suppressor gene, TAX1BP2, in liver cancer |
Investigator(s): |
Ching YP |
Department: |
Pathology |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
04/2007 |
Abstract: |
1. Key issues and problem being
addressed: Liver cancer (hepatocellular carcinoma, HCC) is one of the most
common cancers worldwide. The prognosis of HCC patients is often poor because
of the delay in diagnosis and its high recurrence rate after surgery.
Although the risk factors of HCC, such as hepatitis B and C virus infection,
cirrhosis, and dietary aflatoxin are well established, the genetic mechanisms
in the pathogenesis and tumour progression are poorly defined. Chromosome
instability that leads to clonal expansion of genetically altered cells is a hallmark
of cancer and is highly relevant to hepatocarcinogenesis. Thus
characterization of tumor suppressor genes and elucidation of the mechanisms
of chromosome instability are both major challenges in liver cancer research.
2. Purpose of proposed project: Recently, we have identified and
characterized a novel cellular centrosomal protein, which we have named
TAX1BP2 (Ching et al., 2006, Nature cell Biology 8: 717-24). We have
demonstrated that TAX1BP2 plays an important role in the regulation of
centrosome duplication, and dysregulation of TAX1BP2 may lead to aneuplody of
cells. In our preliminary study, we observed that TAX1BP2 is frequently
underexpressed in human HCC (40%) and the underexpression of TAX1BP2
transcript is significantly associated with a poorer prognosis in terms of
shorter overall survival rates. In addition, TAX1BP2 is localized at the
chromosome locus 1p36, which is also a frequently deleted region in HCC. As
HBV infection is a major risk factor of HCC, particularly in this region and locally,
we have found that the HBV viral oncoprotein HBx interacts with TAX1BP |
List of Research Outputs |
Chin K.T., Xu H., Ching Y.P. and Jin D., Differential subcellular localization and activity of kelch repeat proteins KLHDC1 and KLHDC2, Molecular and Cellular Biochemistry. Springer, 2007, 296: 109-119. |
Chin K.T., Chun C.S., Ching Y.P., Jeang K.T. and Jin D., Human T-cell leukemia virus oncoprotein represses nuclear receptor-dependent transcription by targeting coactivator TAX1BP1, Cancer Research. 2007, 67: 1072-1081. |
Ching Y.P., Leong V.Y.L., Lee M.F., Xu H., Jin D. and Ng I.O.L., P21-activated protein kinase is overexpressed in hepatocellular carcinoma and enhances cancer metastasis involving c-Jun NH2-terminal kinase activation and paxillin phosphorylation. , Cancer Research. 2007, 67: 3601-3608. |
Kok K.H., Ng M.H., Ching Y.P. and Jin D., Human TRBP and PACT directly interact with each other and associated with Dicer to facilitate the production of small interfering RNA , Journal of Biological Chemistry. 2007, 282: 17649-17657. |
Kok
K.H., Ching Y.P. and Jin D., Human TRBP and PACT directly
interact with each other and form a complex with Dicer to promote the
production of small interfering RNA, In: Greg Hannon, Carlo Croce, Scott
Hammond, Keystone Symposium on MicroRNA and Cancer. Keystone Resort, |
Liu
M.H.F., Leong V.Y.L., Ng I.O.L. and Ching Y.P., The tumor suppressive
function of AMPK in hepatocellular carcinoma, Proceedings of the Annual
Meeting of American Association for Cancer Research, |
Researcher
: Chiu J |
Project Title: |
Regulation of [alpha]-fetoprotein gene expression in differentiating and cancer cells |
Investigator(s): |
Chiu J |
Department: |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2002 |
Completion Date: |
12/2006 |
Abstract: |
The project attempts to: (1) Identify and characterize DAS-binding protein (DAP) that regulate [alpha]-fetoprotein expression; (2) Investigate the specific DNA binding activity and biological function of DAP. The biological function will be determined by using various mutant genes, DAP antisense sequence and transcription factor decoys; (3) Identify other genes that are regulated by the DAS cis-element through a computer-assisted analysis of the genomic sequences in GenBank; and (4) Investigate the expression of these candidate genes in F9 cells during differentiation, and in developing liver cells and hepatomas. |
Project Title: |
Biochemical and proteomic analyses of arsenic carcinogenesis |
Investigator(s): |
Chiu J, Leung SY, He Q |
Department: |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2003 |
Abstract: |
To establish and examine the processes of in vitro carcinogenesis induced by arsenic; to identify key elements of oxidative stress that involve in arsenic-induced cell transformation by biochemical and proteomic approaches; to determine which signaling pathway that mediates arsenic-induced cell transformation by proteomic approach. |
List of Research Outputs |
Chiu
J., Apoptosis pathways as targets in cancer
therapy, International Chinese Bioscientist Symposium on Medical and
Pharmacheutical Biotechnology, July 2006, |
Chiu
J., Apoptosis pathways: Targets in cancer
therapy, Frontiers in Biomedical Research, The |
Chiu
J., Proteomic approach to study the
cytotoxicity of dioscin, a saponin of Polygonatum Zanlanscianense
pamp, 2006 World Conference on Chinese Medicine: Charting the Course of
Development, November 23-25, 2006, |
Lau
T.Y. and Chiu J., The
possible role of cytokeratin |
Li G.
and Chiu J., Activations of
Stat1 and Erk are involbed in the resveratrol-induced human esophageal cancer
cell apoptosis, 2006 AACR International Conference on Frontiers in Cancer
Prevention Research, November 11-15, 2006, |
Li M., Chiu J., Mossman B.T. and Fukagawa
N.K., Down-regulation of manganese-superoxide dismutase through
phosphorylation of FOXO |
Lo A.C.Y., Hung K.L., Cheung K.H.A., He Q., Chiu J., Chung S.S.M. and Chung S.K., Aldose reductase-deficient mice are protected from iron- and transferrin-related oxidative stress and cerebral ischemic injury, 36th Annual Meeting of the Society for Neuroscience 2006. |
Lok C.N., Ho C.M., Chen R., He Q., Yu W.Y., Sun H., Tam P.K.H. and Chiu J., Proteomic analysis of the mode of antibacterial action of silver nanoparticles, Gordon Research Conference, Metal in Medicine, Oxford, United Kingdom, July 9-14, 2006. |
Tian J., Wong K.K.Y., Ho C.M., Lok C.N., Yu W.Y., Che C.M., Chiu J. and Tam P.K.H., Topical delivery of silver nanoparticles promotes wound healing, ChemMedChem. 2007, 2: 129-136. |
Wang Y., Chiu J. and He Q., Bioinformatic application in proteomic research on biomarker discovery and drug target validation, Current Bioinformatics. 2007, 2: 11-20. |
Wang
Y., He Q., Sun R.W.Y., Che C.M. and Chiu J., Cellular pharmacological
properties of gold(III) porphyrin |
Wang Y., Chiu J. and He Q.Y., Proteomics approach to illustrate drug action mechanisms, Current Drug Discovery Technologies. 2006, 3: 199-209. |
Wang Y., He Q., Chen H. and Chiu J., Synergistic effects of retinoic acid and tamoxifen on human breast cancer cells: Proteomic characterization, Experimental Cell Research. 2006, 313: 357-368. |
Wong K.K.Y., Tian J., Ho C.M., Lok C.N., Che C.M., Chiu J. and Tam P.K.H., Topical delivery of silver nanoparticles reduces systemic inflammation of burn and promotes wound healing, Nanomedicine : nanotechnology, biology, and medicine. 2006, 2(4): 306. |
Zhou Y., Bhata I., He Q.Y., Cheung P.T. and Chiu J., Hypoxia-ischemia induces dephosphorylation of collapsin mediator proteins ( CRMPs) in neonatal mice through down regulation of Cdk5/p35, 46th Annual meeting of The American Society for Cell Biology, December 9-13, 2006, San Diego, California, U. S. A.. 2006. |
Researcher
: Chiu K |
List of Research Outputs |
Chan H.C., Chang R.C.C., Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y. and So K.F., Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma, Experimental Neurology. 2006, 203: 269-273. |
Chiu K., Ji J., Yu M.S., So K.F. and Chang R.C.C., Activation of microglia/macrophages determines the fate of retinal ganglion cell survival in rat chronic ocular hypertension model, Neurosignals. 2006, 15: 139. |
Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y., Chang R.C.C. and So K.F., Neuroprotective effect of Lycium barbarum in rat chronic ocular hypertension model via immunomodulation of macrophages/microglia, Neurosignals. 2006, 15: 145. |
Yeung
S.C., Chiu K., So K.F. and Chang R.C.C., The effects of
intravitreal injection of IL-10 on the survival of retinal ganglion cells in
the rat glaucoma model, The 5th Asia-Pacific symposium on Neural
Regeneration, |
List of Research Outputs |
Chu Q.,
Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Garlic derivatives inhibit
prostate cancer cell growth and invasion through restoration of E-cadherin
expression, 8th Asian Congress of Urology, |
Chu Q., Lee D.T.W., Tsao G.S.W., Wang X. and Wong Y.C., S-allycysteine, a water-soluble garlic derivative, suppresses the growth of a human androgen-independent prostate cancer xenograft, CWR22R, under in vivo conditions, BJU International. 2006, 99: 925-932. |
Chu Q., S-allylcystein (SAC) and S-allylmercaptocysteine (SAMC), water soluble garlic derivatives, suppress growth and invasion of androgen-independent prostate cancer, under in vitro and in vivo conditions. 2007, 163 pages. |
Wong
Y.C., Chu Q., Lee D.T.W. and Wang X., S-allylcysteine (SAC), a garlic
derivative, suppresses the growth fo androgen-independent prostate cancer
xenograft under in vivo conditiion, AACR Special Conference in Cancer
Research: Innovations in Prostate Cancer Research, December 6-9, 2006, |
List of Research Outputs |
Chu Q.,
Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Garlic derivatives inhibit
prostate cancer cell growth and invasion through restoration of E-cadherin
expression, 8th Asian Congress of Urology, |
Chu Q., Lee D.T.W., Tsao G.S.W., Wang X. and Wong Y.C., S-allycysteine, a water-soluble garlic derivative, suppresses the growth of a human androgen-independent prostate cancer xenograft, CWR22R, under in vivo conditions, BJU International. 2006, 99: 925-932. |
Chu Q., S-allylcystein (SAC) and S-allylmercaptocysteine (SAMC), water soluble garlic derivatives, suppress growth and invasion of androgen-independent prostate cancer, under in vitro and in vivo conditions. 2007, 163 pages. |
Wong
Y.C., Chu Q., Lee D.T.W. and Wang X., S-allylcysteine (SAC), a garlic
derivative, suppresses the growth fo androgen-independent prostate cancer
xenograft under in vivo conditiion, AACR Special Conference in Cancer
Research: Innovations in Prostate Cancer Research, December 6-9, 2006, |
List of Research Outputs |
Chu T.H. and Wu W., Nitric oxide synthase inhibitor attenuates number of regenerating spinal motoneurons in adult rats, NeuroReport. 2006, 17: 969-973. |
Su H., Chu T.H. and Wu W., Lithium enhances proliferation and neuronal differentiation of neural progenitor cells in vitro and after transplantation into the adult rat spinal cord, Experimental Neurology. 2007, 206: 296-307. |
List of Research Outputs |
Chu T.H. and Wu W., Nitric oxide synthase inhibitor attenuates number of regenerating spinal motoneurons in adult rats, NeuroReport. 2006, 17: 969-973. |
Su H., Chu T.H. and Wu W., Lithium enhances proliferation and neuronal differentiation of neural progenitor cells in vitro and after transplantation into the adult rat spinal cord, Experimental Neurology. 2007, 206: 296-307. |
Researcher
: Chua CW |
List of Research Outputs |
Howard E.W., Chua C.W., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Potent suppression of distant metastasis by garlic compound S-allylmercaptocysteine in an in vivo androgen independent prostate cancer model, AACR Special Conference in Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006, San Francisco, U. S. A.. 2006, A1. |
Yuen
H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Id proteins expression in
prostate cancer: high-level expression of Id |
Yuen H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Significance of TWIST and E-cadherin expression in the metastatic progression of prostatic cancer, Histopathology. 2007, 50(5): 648-58. |
Zhang Z., Xie D., Li X., Wong Y.C., Xin D., Guan X.Y., Chua C.W., Leung S.C., Na Y. and Wang X., Significance of TWIST expression and its association with E-cadherin in bladder cancer., Human Pathology. 2007, 38: 598-606. |
Researcher
: Chua CW |
List of Research Outputs |
Howard E.W., Chua C.W., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Potent suppression of distant metastasis by garlic compound S-allylmercaptocysteine in an in vivo androgen independent prostate cancer model, AACR Special Conference in Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006, San Francisco, U. S. A.. 2006, A1. |
Yuen
H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Id proteins expression in
prostate cancer: high-level expression of Id |
Yuen H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Significance of TWIST and E-cadherin expression in the metastatic progression of prostatic cancer, Histopathology. 2007, 50(5): 648-58. |
Zhang Z., Xie D., Li X., Wong Y.C., Xin D., Guan X.Y., Chua C.W., Leung S.C., Na Y. and Wang X., Significance of TWIST expression and its association with E-cadherin in bladder cancer., Human Pathology. 2007, 38: 598-606. |
Researcher
: Chung SK |
Project Title: |
Characterization of endothelin-1 gene manipulated mice for ischemic stroke, a leading cause of death and disability |
Investigator(s): |
Chung SK |
Department: |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2002 |
Completion Date: |
08/2006 |
Abstract: |
The project attempts to determine if the ET-1 knockout mice have more severe ischemic brain injuries. The absolute and regional cerebral blood flow, neurological deficit, infarct volume and the detailed molecular signals will be determined. |
Project Title: |
Molecular mechanisms of dementia associated with aging-related diseases, alzheimer's and multi-infarct |
Investigator(s): |
Chung SK |
Department: |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
12/2003 |
Abstract: |
To characterize of GET, TET or ETKO mice with human Swedish mutation in APP gene; to determine amyloidogenesis and neuropathological features in ET-1/mutant APPYAC brain; to determine memory deficit and brain activity in ET-1/mutant APPYAC mice; to determine the role of infarct and mutant APP on oxidative stress and neuropathogical features; to determine efficacy of ECE inhibitor and ETA and ETB receptors antagonists for neurotoxicity. |
Project Title: |
Genetic approaches to understand the pathogenesis of diabetic neuropathy: common, debilitating disease |
Investigator(s): |
Chung SK |
Department: |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2005 |
Abstract: |
To determine contributions of AR and SD to the increased oxidative stress in diabetic nervous tissues; to determine the relationship between AR activity and the activation of NF-[kappa]B and PKC; to determine the contributions of vascular and nervous tissues to the diabetes-induced MNCV deficit; to determine the role of AR in the pathogenesis of chronic neuropathy by using the Thy1-YFP transgenic mice. |
Project Title: |
Conditional knockout of osmotic responsive element binding protein, OREBP/NFAT5/TonEBP, to study its role in brain edema and infarct after ischemic stroke: a leading cause of mortality and morbidity |
Investigator(s): |
Chung SK |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2007 |
Abstract: |
To determine the role of OREBP in brain water content, edema and infarct after ischemic stroke using two lines of heterozygous OREBP knockout mice derived from 2-independent ES cells; to determine the molecular signals downstream to OREBP in contributing to astrocyte and neruronal cell death during ischemic and hypertonic stress using primary astrocyte and neuronal cultures from embryos of homozygous OREBP-deficient mice; to investigate the effects of conditional deletion of OREBP in astrocytes or neurons on brain edema and infarct after ischemic stroke. |
List of Research Outputs |
Chan
K.C., Yau T.O., Ng I.O.L. and Chung S.K., Deleted in liver cancer
2 (DLC2) knockout mice show fast response to heat induced-pain, 11th
Postgraduate symposium 2006, |
Chan W.H., Chung S.K. and Chung S.S.M., Contribution of polyol-pathway to slow-developing diabetic cataract, International Symposium on Healthy Aging: A Gloval Challenge for the 21st Century. 2006. |
Cheung
K.H.A., Lo A.C.Y., Chung S.S.M. and Chung S.K., Deletion of aldose
reductase gene protects against neuronal death and edema in retina after
transient ischemia by preventing oxidative stress, 11th Research
Postgraduate Symposium 2006, |
Chu Y.S.J., Chung C.K.S., Lam A.K.M., Tam S., Chung S.K. and Chow B.K.C., Phenotypes Developed in Secretin Receptor-Null Mice Indicated a Role for Secretin in Regulating Renal Water Reabsorption, Molecular and Cellular Biology. 2007, 27: 2499-2511. |
Chung S.K., Molecular genetics of diabetic microangiopathy, Fourth International Huaxia Congress of Endocrinology. 2006. |
Chung S.K., Role of aldose reductase in diabetic and ischemic retinopathy, Association for Ocular Pharmacology and Therapeutics. 2007. |
Chung S.K., Targeting of diabetic neuropathy: the role of aldose reductase, AGE and oxidative stress, 21st Annual Meeting of Japanese Society of Diabetic Complications. 2006. |
Chung S.K., The use of Thy1.2-YFP transgenic reporter mice for understanding the nerve development and degeneration, Special Lecture for School of Brain Function, The University of Hong Kong, The Chinese University of Hong Kong, HKUST and Baptist University. HKU, CUHK, HKUST, Baptist U, 2006. |
Chung S.K. and Chung S.S.M., Transgenic and knockout mouse models of diabetic neuropathy, The Clinical Management of Diabetic Neuropathy, Second Edition. 2007. |
Ho H.T.B., Ko C.B., Cheung A.K.H., Lam A.K.M., Tam S., Chung S.K. and Chung S.S.M., Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice, Kidney International. 2007, 72: 63-71. |
Huang P., Jiang Z., Teng S., Wong Y.C., Frohman M.A., Chung S.K. and Chung S.S.M., Synergism between phospholipase D2 and sorbitol accumulation in diabetic cataract formation through modulation of Na, K-A TPase activity and osmotic stress, Experimental Eye Research. 2006, 83: 939-948. |
Hung
K.L., Lo A.C.Y., Lamb B.T. and Chung S.K., Astrocytic
over-expression of endothelin-1 accelerates sensorimotor gating deficit,
prolonged habituation and spatial reference memory impairment in APP mutant
mice, 11th Research Postgraduate Symposium 2006, |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 11th Reserach Postgraduate symposium 2006. |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 36th Annual Meeting of the Society for Neuroscience 2006. |
Lo A.C.Y., Hung K.L., Cheung K.H.A., He Q., Chiu J., Chung S.S.M. and Chung S.K., Aldose reductase-deficient mice are protected from iron- and transferrin-related oxidative stress and cerebral ischemic injury, 36th Annual Meeting of the Society for Neuroscience 2006. |
Mak M.C., Lo A.C.Y., Lam A.K.M., Chung S.S.M. and Chung S.K., NFAT5 deficiency increases the severity of neuronal cell death in ischemic , 36th Annual Meeting of the Society for Neuroscience 2006. |
Yamagishi S.I., Ogasawara S., Sugawara
A., Chung S.S.M., Chung S.K. and Yagihashi S.,
Glycemic control is crucial for the treatment of aldose reductase inhibitor
(ARI), Lessons from Diabetic AR-deficient Mice, |
Yang J.Y., Tam W.Y., Tam S., Guo H., Wu X., Li G., Chau J.F., Klein J.D., Chung S.K., Sands J.M. and Chung S.S.M., Genetic restoration of aldose reductase to the collecting tubules restores maturation of the urine concentrating mechanism, American Journal of Physiology Renal Physiology. 2006, 291: F186-F195. |
Researcher
: Deng W |
Project Title: |
Dynamics of numerical chromosome instability in human cells undergoing immortalization |
Investigator(s): |
Deng W, Cheung A |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
10/2005 |
Abstract: |
Chromosome instability is a major form of genomic instability that helps drive development of human cancer [1]. Numerical chromosome instability is manifested by dynamic changes of chromosome numbers, i.e., losses or gains of whole-chromosomes, which are almost ubiquitous in human cancer. Since thousands of genes can be affected by whole-chromosome losses or gains, this type of instability is more efficient in inducing genetic alterations than mere gene mutations. However, to date, the mechanisms underlying numerical chromosome instability still remain unclear. The objectives of this study are: (1) To test whether telomere dysfunction induces numerical chromosome instability It has been a long-standing mystery why pre-immortal, immortalized and cancer cells universally have nonrandom numerical chromosome abnormalities. Recent studies on telomeres, the terminal structure crucial for protecting chromosomes against end-fusions, provide some hints. Since telomere lengths on individual chromosomes are recently found to be highly heterogeneous, we hypothesize that the chromosomes with the shortest telomeres are most susceptible to fusion-bridge formation; and the bridge could be broken in microtubule due to the pulling forces of the fused chromosomes during subsequent cell division. This would result in preferential losses or gains of specific chromosomes with dysfunctional telomeres. (2) To test whether aneuploidy promotes numerical chromosome instability It is hypothesized that the initial whole-chromosome losses or gains (generally termed aneuploidy) may trigger asymmetrical production of mitotic elements, which then destabilizes the karyotype and generates greater aneuploidy, causing a chain-reaction or autocatalysis of numerical chromosome instability [2]. The hypothesis predicts that numerical chromosome instability would increase with the degree of aneuploidy and cell population doublings. This would drive the continued progression of numerical chromosome instability. (3) To test whether centrosome abnormality contributes to numerical chromosome instability During mitosis, two duplicated centrosomes normally serve as organizing centers of the two poles of the mitotic spindle to provide the machinery for the correct separation of two groups of chromosomes. We hypothesize that multiple centrosomes may cause multi-polar mitosis and thus failed segregation of a whole set of chromosomes. We therefore propose to investigate the dynamics of centrosome abnormality and the subsequent dynamic generation of nearly whole sets of chromosome number abnormalities in the process of immortalization to better define their causal relationship. Reference: 1.Lengauer,C., Kinzler,K.W. and Vogelstein,B. (1998). Genetic instabilities in human cancers. Nature, 396:643-649. 2.Li, R., Sonik,A., Stindl,R., Rasnick,D., and Duesberg,P. (2000). Aneuploidy vs. gene mutation hypothesis of cancer: recent study claims mutation but is found to support aneuploidy. Proc. Natl. Acad. Sci. U. S. A., 97:3236-3241. |
List of Research Outputs |
Cheung A., Cheung P.Y., Deng W. and Tsao G.S.W., Immortalization of human esophageal epithelial cells by human telomerase reverse transcriptase (hTERT), Proceedings o the American Association for Cancer Research Annual Meeting, April 14-18, Los Angeles, U. S. A.. 2007, No. 4268. |
Deng W., Award for Outstanding Research Postgraduate Student (September 2004 - August 2005). 2006. |
Researcher
: Di K |
List of Research Outputs |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-Beta1 sensitivity in prostate epithelial cells, Biology of the Cell. 2006, 98(9): 523-533. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-β1 sensitivity in prostate epithelial cells. , Biology of the Cell. 2006, 98: 523-533. |
Researcher
: Di K |
List of Research Outputs |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-Beta1 sensitivity in prostate epithelial cells, Biology of the Cell. 2006, 98(9): 523-533. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-β1 sensitivity in prostate epithelial cells. , Biology of the Cell. 2006, 98: 523-533. |
Researcher
: Du Y |
List of Research Outputs |
Du
Y. and Yip
H.K.F., Expression Patterns And Localization Of Dna-binding Protein
Inhibitor Id |
Researcher
: Ellis-Behnke RG |
List of Research Outputs |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., So K.F. and Wu E.X., Chronic Injury Visualization of Regenerating Axons in Vivo in Hamster Optic Tract Transection Utilizing a 7 Tesla FMRI and a Nano Contrast Agent, Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
Ellis-Behnke
R.G., From Nano Neuro Knitting To Immediate
Hemostasis , 27th Blankenese Conference "Routes to Therapy: From Stem
Cell Tailoring to Nano Knitting," |
Ellis-Behnke R.G., Intersection of Nanotechnology and Healthcare, MIT Healthcare Industries Conference. 2007. |
Ellis-Behnke
R.G., Keynote: From Nano Neuro Knitting to
Crystal Clear Surgery, Nanomedicine, |
Ellis-Behnke R.G., Keynote: Using Nanotechology To Repair The Brain, Accelrys Seminar Series on Nanodesign - Revolutionizing Healthcare and Medicine through Nanotechnology. 2006. |
Ellis-Behnke R.G., So K.F. and Zhang S., Molecular repair of the brain using self-assembling peptides., Chimica Oggi. 2006, 24: 12-14. |
Ellis-Behnke R.G., So K.F. and Zhang S., Molecular repair of the brain using self-assembling peptides, Chemistry Today. 2006, 24(4): 42-43. |
Ellis-Behnke R.G., Teather L.A. and So K.F., Molecular restoration of the body: nano neuro knitting for brain repair, British Anti-Ageing Medical Journal. 2006, 4: 35-37. |
Ellis-Behnke R.G., Nano Neuro Knitting And Hemostasis, National Nanotechnology Initiative of the National Science Foundation. 2006. |
Ellis-Behnke R.G., Liang Y., You S.W., Tay D.K.C., Zhang S., Schneider G.E. and So K.F., Nano Neuro Knitting Nanotechnology For Hemostasis, Brain And Organ Repair, Inaugural Tissue Engineering (SuTEN), Australia, November 23, 2006. |
Ellis-Behnke R.G., Nano Neuro Knitting Of The Spinal Cord, 2nd International Spinal Cord Injury Treatments and Trials Symposium. 2006. |
Ellis-Behnke R.G., Nano Neuro Knitting, 13th Annual Optic Nerve Rescue and Restoration Think Tank. 2006. |
Ellis-Behnke R.G., Nano Neuro Knitting, 2nd International SBE Conference on Bioengineering and Nanotechnology. 2006. |
Ellis-Behnke
R.G., Liang
Y., You S., Tay D.K.C., Zhang S., Wu W., So
K.F. and Schneider G.E., Nano
Neuro Knitting: Using Nanotechnology To Repair The CNS, NSTI Nanotech and
Bio Nano, |
Ellis-Behnke
R.G., Nano Neuro Knitting: using
nanotechnology to repair the Central Nervous System , NSTI Nanotech and
Bio Nano conference, San Francisco |
Ellis-Behnke
R.G., Nano Neuro Technology To Repair The
Brain, 2nd Conference of the |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., Wu W., Schneider G.E., Zhang S. and So K.F., Nano hemostat solution: Immediate hemostasis at the nanoscale, Nanomedicine: Nanotechnology, Biology and Medicine. 2006, 2(4): 207-215. |
Ellis-Behnke
R.G., Nanotechnology And CNS Repair, 5th |
Ellis-Behnke
R.G., Liang
Y., Wu W., You S.W., Schneider G.
and So K.F., Nanotechnology and CNS
repair, The 5th Asia-Pacific Symposium on Neural Regeneration, |
Ellis-Behnke
R.G., Nanotechnology and Tissue
Bioengineering, Annual Meeting of the Association for Research in Vision
and Ophthalmology (ARVO) Nanotechnology Symposium, Ft Lauderdale |
Ellis-Behnke R.G., Neurology Editor, Nanomedicine: Nanotechnology, Biology, and Medicine. Elsevier, 2007. |
Ellis-Behnke
R.G., Plenary Speaker: Nano Neuro Knitting, |
Ellis-Behnke
R.G., Symposium Chair – Neurology
Nanomedicine, 2nd Conference of the |
Ellis-Behnke R.G., So K.F. and Schneider G.E., The 4 P's of CNS regeneration: A framework for approaching the repair of neural trauma using nanotechnology and combination therapies, Society for Neuroscience. 2006, Program No. 228.23. |
Ellis-Behnke R.G., The 4 Ps of CNS regeneration, 1st Workshop on Regeneration/Repair in Nervous System, Juvenile Diabetes Research Foundation (JDRF) and European Association for the Study of Diabetes (EASD). 2006. |
Ellis-Behnke
R.G., The Intersection Of Nanotechnology And
Medicine: From Nano Neuro Knitting To Crystal Clear Surgery, MIT |
Ellis-Behnke
R.G., The Intersection of Nanotechnology and
Healthcare, Annual Meeting of the American Intellectual Property Law
Association, |
Ellis-Behnke
R.G., The Intersection of Nanotechnology and
Healthcare, MIT Healthcare Industries Conference, |
Ellis-Behnke R.G., Using Nanotechnology To Repair The Body, Nanotechnology 2006. 2006. |
Ellis-Behnke R.G., Using Nanotechnology To Repair The Body, Xiangshan International Nanomedicine Conference. 2006. |
Ellis-Behnke R.G., Using nanotechnology for surgical interventions, 1st IEEE International Conference on Nano/Molecular Medicine and Engineering. 2006. |
Liang Y., Ellis-Behnke R.G., Tay D.K.C., You S., Schneider G.E. and So K.F., Creation of a more permissive environment using self-assembling peptide nanofiber scaffold in combination with chondroitinase ABC for brain lesion repair and functional return of vision, Society for Neuroscience. 2006, Program No. 720.6. |
Nan Y., Xiao C.X., Zhang Y., Chen B.Y., Yu E.H., Ellis-Behnke R.G., So K.F., Lewis G.P., Fisher S.K. and Pu M., Visual response properties of cat retinal ganglion cells after retinal detachment, Association for Research in Vision and Ophthalmology, Ft Lauderdale USA. 2007, Program No. 5754 No. B73. |
Schneider G.E., Ellis-Behnke R.G., Liang Y., Lui Kau K.W.F., Tay D.K.C. and So K.F., Behavioral testing and analysis of the hamster visual system, Nature Protocols. 2006, 1(4): 1898-1901. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of Self-assembling Peptide Nanofiber Scaffold and Chondroitinase-ABC Appear to Create a More Permissive Environment in Optic Tract Brain Lesion Repair Resulting in Return of Vision, Annual Meeting of the Association for Vision and Research in Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of self-assembling peptide nanofiber scaffold and chondroitinase-ABC appear to create a more permissive environment in optic tract brain lesion repair resulting in return of vision, Association for Research in Vision and Ophthalmology, Ft Lauderdale USA, . 2007, Poster No. 3168. |
So
K.F. and Ellis-Behnke R.G.,
Nano neuro knitting: peptide nanofiber scaffold for brain repair and axon
regeneration with functional return of vision, International Symposium on
Applied Neuroscience: Recovering from Brain Trauma, May 4-5, 2007, |
So K.F. and Ellis-Behnke R.G., Nanomedicine for CNS regeneration, wound healing, and instant hemostasis, 第二届全国組织工程干細胞与神经再生學術会議論文集. 2006, 4. |
So K.F., Liang Y., You S., Tay D.K.C., Schneider G.E. and Ellis-Behnke R.G., Promotion of axonal growth by CNTF in a permissive environment of self-assembling peptide nanofiber scaffold for brain lesion repair and functional return of vision in adult hamsters, Society for Neuroscience. 2006, Program No. 522.14. |
Researcher
: Fu Q |
List of Research Outputs |
Fu Q., Wu W., Hu B., Chan S.Y.M., Shao Z., Pepinsky R.B., Mi S. and So K.F., LINGO-1 Antagonists protects retinal ganglion cells in a chronic hypertensive model of glaucoma, the 29th Annual Meeting of the Japan Neuroscience Society, Kyoto, Japan, July 19-21, 2006. S119 No. PS1P-E073. |
Researcher
: Fung KL |
List of Research Outputs |
Fung K.L., Cheung H.W., Wong H.L., Yuen H.F., Ling M.T., Chan K.W., Wong Y.C., Cheung A. and Wang X., MAD2 expression and its significance in mitotic checkpoint control in testicular germ cell tumour. , Biochimica et Biophysica Acta - Molecular Cell Research . 2007, 1773: 821-832. |
Fung K.L., Cheung H.W., Ling M.T., Cheung A., Wong Y.C. and Wang X., Role of MEK/ERK pathway in the MAD2-mediated cisplatin sensitivity in testicular germ cell tumour cells, British Journal of Cancer. 2006, 95: 475-484. |
Researcher
: Guo J |
List of Research Outputs |
Guo J., Zeng Y.S., Liang Y., Wang L., Su H. and Wu W., Cyclosporine affects the proliferation and differentiation of neural stem cells in culture, NeuroReport. 2007, 18(9): 863-868. |
Researcher
: Ho MCT |
List of Research Outputs |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcholic fatty liver disease (NAFLD) not
requiring a high fat diet, Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcoholic fatty liver disease (NAFLD) not
requiring a high fat diet 1275 Suppl, Hepatology. 2006, 44(4): |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorate pathological changes, oxidative stress and pro-inflammatory
markrs in an animal model of non-alcoholic fatty liver disease [NAFLD], Hepatology.
2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.H.Y., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorated pathological changes, oxidative stress and
pro-inflammatory markers in an animal model of non-alcoholic fatty liver
disease (NAFLD) animal model 1063 Suppl 1, Hepatology . 2006, 44(4): |
Researcher
: Ho YS |
List of Research Outputs |
Chang R.C.C., Lai S.W., Yu M.S., Cheung Y.T. and Ho Y.S., The impact from understanding the biological mechanisms of neurodegeneration in Alzheimer's disease to the development of neuroprotective agents, Asian Journal of Gerontology & Geriatrics. 2007, 2(1): 31. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Alkaline extract of lycium barbarum protects against beta-amyloid peptide neurotoxicity in rat cortical neurons by activation of AKT, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 126-127 No. P-C36. |
Ho Y.S.,
Yu M.S., So K.F., Yuen W.H. and Chang R.C.C., Attenuation of unfolded
protein responses by reducing stress: an example of neuroprotective effect of
Lycium barbarum, 2006 Hong Kong-Macau Postgraduate Symposium on
Chinese Medicine, August 17, 2006, |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Characterizing the Neuroprotective Effects of Alkaline Extract of Lycium Barbarumon b-amyloid Peptide Neurotoxicity , Brain Research . 2007, 1158: 123-134. |
Ho Y.S., Yu M.S., Lai S.W., So K.F. and Chang R.C.C., Glycoconjugates from anti-aging Lycium Barbarum protect primary cortical neurons from beta-amyloid neurotoxicity, Second International Symposium on Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4, 2007 Hong Kong. 2007, 53 P2. |
Ho Y.S., Yu M.S., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity, Society for Neuroscience. 2006, Program No. 826.10. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Neuroprotective effects of anti-aging Lycium barbarum by a novel extraction method, 2006 World Congress on Chinese Medicine: Charting the Course of Development, Hong Kong, November 23-25, 2006. 247. |
Yik
S.Y., Ho Y.S., Lai S.W., So K.F. and Chang R.C.C., Significance of dsRNA
produced by viral infection in neurodegeneration, Second International
Symposium on Healthy Aging: Meeting the Challenges of an Aging Population,
March 3-4, 2007, |
Yu M.S., Ho Y.S., So K.F., Yuen W.H. and Chang R.C.C., Cytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulum, Neurosignals. 2006, 15: 145. |
Researcher
: Howard EW |
List of Research Outputs |
Howard E.W., Chua C.W., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Potent suppression of distant metastasis by garlic compound S-allylmercaptocysteine in an in vivo androgen independent prostate cancer model, AACR Special Conference in Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006, San Francisco, U. S. A.. 2006, A1. |
Researcher
: Hu B |
List of Research Outputs |
Fu Q., Wu W., Hu B., Chan S.Y.M., Shao Z., Pepinsky R.B., Mi S. and So K.F., LINGO-1 Antagonists protects retinal ganglion cells in a chronic hypertensive model of glaucoma, the 29th Annual Meeting of the Japan Neuroscience Society, Kyoto, Japan, July 19-21, 2006. S119 No. PS1P-E073. |
Researcher
: Hung KL |
List of Research Outputs |
Hung K.L., Lo A.C.Y., Lamb B.T. and
Chung S.K., Astrocytic
over-expression of endothelin-1 accelerates sensorimotor gating deficit,
prolonged habituation and spatial reference memory impairment in APP mutant
mice, 11th Research Postgraduate Symposium 2006, |
Lo A.C.Y., Hung K.L., Cheung K.H.A., He Q., Chiu J., Chung S.S.M. and Chung S.K., Aldose reductase-deficient mice are protected from iron- and transferrin-related oxidative stress and cerebral ischemic injury, 36th Annual Meeting of the Society for Neuroscience 2006. |
Researcher
: Ip KC |
List of Research Outputs |
Chan H.C., Chang R.C.C., Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y. and So K.F., Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma, Experimental Neurology. 2006, 203: 269-273. |
Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y., Chang R.C.C. and So K.F., Neuroprotective effect of Lycium barbarum in rat chronic ocular hypertension model via immunomodulation of macrophages/microglia, Neurosignals. 2006, 15: 145. |
Researcher
: Ip KC |
List of Research Outputs |
Chan H.C., Chang R.C.C., Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y. and So K.F., Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma, Experimental Neurology. 2006, 203: 269-273. |
Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y., Chang R.C.C. and So K.F., Neuroprotective effect of Lycium barbarum in rat chronic ocular hypertension model via immunomodulation of macrophages/microglia, Neurosignals. 2006, 15: 145. |
Researcher
: Ji J |
List of Research Outputs |
Chiu K., Ji J., Yu M.S., So K.F. and Chang R.C.C., Activation of microglia/macrophages determines the fate of retinal ganglion cell survival in rat chronic ocular hypertension model, Neurosignals. 2006, 15: 139. |
List of Research Outputs |
Yu M.S., Lai S.W., Suen K.C., Kwok N.S., So K.F., Hugon J. and Chang R.C.C., Extracellular accumulation of beta-amyloid peptides induces apoptosis in cultured neurons via a mechanism independent of unfolded protein responses, Neurosignals. 2006, 15: 141. |
Researcher
: Kwok WK |
List of Research Outputs |
Yuen H.F., Chan Y.P., Wong M.L.Y., Kwok W.K., Chan K.K., Lee P.Y., Srivastava G., Law S.Y.K., Wong Y.C., Wang X. and Chan K.W., Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis, Journal of Clinical Pathology. 2006, 60(5): 510-514. |
Researcher
: Lai SW |
List of Research Outputs |
Chang R.C.C., Lai S.W. and Yu M.S., Collapse of endoplasmic reticulum as a new mechanism mediating beta-amyloid peptide-triggered neurotoxicity, Alzheimer's and Parkinson's Diseases: Progress and New Perspectives, 8th International Conference AD/PD, Salzburg, Austria, March 14-18, 2007. 4(suppl 1): 61-62. |
Chang R.C.C., Suen A.K.C., Yu M.S., Lai S.W., Cheung Y.T. and Hugon J., Roles of protein translation control in neurodegeneration of Alzheimer's Disease, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientifiic Meeting of The Hong Kong society of Neurosciences, Nov. 30 - Dec. 2, 2006. 7 No. S6. |
Chang R.C.C., Yu M.S. and Lai S.W., Significance of molecular signaling for protein translation control in neurodegenerative diseases, Neurosignals. 2007, 15: 249-258. |
Chang R.C.C., Lai S.W., Yu M.S., Cheung Y.T. and Ho Y.S., The impact from understanding the biological mechanisms of neurodegeneration in Alzheimer's disease to the development of neuroprotective agents, Asian Journal of Gerontology & Geriatrics. 2007, 2(1): 31. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Alkaline extract of lycium barbarum protects against beta-amyloid peptide neurotoxicity in rat cortical neurons by activation of AKT, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 126-127 No. P-C36. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Characterizing the Neuroprotective Effects of Alkaline Extract of Lycium Barbarumon b-amyloid Peptide Neurotoxicity , Brain Research . 2007, 1158: 123-134. |
Ho Y.S., Yu M.S., Lai S.W., So K.F. and Chang R.C.C., Glycoconjugates from anti-aging Lycium Barbarum protect primary cortical neurons from beta-amyloid neurotoxicity, Second International Symposium on Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4, 2007 Hong Kong. 2007, 53 P2. |
Ho Y.S., Yu M.S., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity, Society for Neuroscience. 2006, Program No. 826.10. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Neuroprotective effects of anti-aging Lycium barbarum by a novel extraction method, 2006 World Congress on Chinese Medicine: Charting the Course of Development, Hong Kong, November 23-25, 2006. 247. |
Lai S.W., So K.F. and Chang R.C.C., Aggregation/collapse of endoplasmic retiulum induced by Ab subsequently undergo autophagy, Second International Symposium on Healthy Aging; Meeting the Challenges of an Aging Population, March 3-4, 2007, Hong Kong. 2007, 53 P4. |
Lai S.W., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Neuroprotective effects of the gandoderma lucidum aqueous extract against beta-amyloid peptide-induced neurotoxicity, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006 . 2006, 97 No. P-B36. |
Lai S.W., Yu M.S., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Potential neuroprotective agent from botanical extract: An experience of using Verbena officinalisagainst b-amyloid peptide neurotoxicity, Neurosignals. 2006, 15: 146. |
Lai S.W., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., The aqueous extract from anti-aging Ganoderma lucidum inhibits beta-amyloid peptide-induced neurotoxicity, Society for Neuroscience. 2006, Program No. 826.11. |
Lau K.W., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma. Does it differentiate them?, The 4th Congress of the Federation of Asian-Oceanian Neuroscience societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 97-98 No. P-B37. |
Lau
K.W., Lai S.W., So K.F. and Chang R.C.C., Impact of retinoic acid on
SH-SY5Y neuroblastoma to neurotoxins, Second International Symposiunm on
Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4,
2007, |
Yik
S.Y., Ho Y.S., Lai S.W., So K.F. and Chang R.C.C., Significance of dsRNA
produced by viral infection in neurodegeneration, Second International
Symposium on Healthy Aging: Meeting the Challenges of an Aging Population,
March 3-4, 2007, |
Yu M.S., Lai S.W., Suen K.A., So K.F., Hugon J. and Chang R.C.C., Absence of unfolded protein responses in extracellular beta-amyloid peptide-induced neuronal apoptosis, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 129 No. P-C41. |
Yu M.S., Lai S.W., Suen K.C., Kwok N.S., So K.F., Hugon J. and Chang R.C.C., Extracellular accumulation of beta-amyloid peptides induces apoptosis in cultured neurons via a mechanism independent of unfolded protein responses, Neurosignals. 2006, 15: 141. |
Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Protein kinases as technological platforms to screen neuroprotective agents from chinese medicine, Neurosignals. 2006, 15: 133. |
Researcher
: Lam AKM |
Project Title: |
Investigating the role of Epac in the differentiation of mouse and human embryonic stem cells into insulin-producing cells |
Investigator(s): |
Lam AKM, Chung SK, Lam KSL |
Department: |
Medical Faculty |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
09/2006 |
Abstract: |
In type 1 and late stage of type 2
diabetes, blood glucose level is dependent on the hormones of the pancreas,
insulin, which is released from pancreatic beta cell. Insulin secretion from
the beta cells is regulated positively and negatively by many intracellular
signals generated by various factors, including nutrients, hormones and
neurotransmitters. cAMP is thought to be a most critical intracellular signal
in the mechanism of insulin secretion (1,2). Glucose is believed to act
principally to stimulate mitochondrial ATP synthesis, leading to the closure
of ATP-sensitive K channel, cell depolarization, calcium influx and
exocytosis.GLP-1 is an insulinotropic hormone with blood glucose-lowering
properties secreted by endocrine L cells of the intestinal tract. Binding of
GLP-1 to the GLP-1 receptor stimulate the production of cAMP and the increase
in intracellular Ca2+ for insulin secretion in pancreatic beta cells. Epac is
a novel cAMP regulated guanine exchange factor for the Ras-like small GTPase
Rap1 and Rap2 (3,4). It was shown that the GEF activity of Epac was strongly
induced by cAMP besides protein kinase A. There are two isoforms of Epac,
Epac1 and Epac2, in the mammalian cell. Both isoforms are expressed in
pancreatic beta-cells (5) and is suggested to mediate the stimulatory actions
of GLP-1 on Ca2+-dependent insulin secretion. Treatment with antisense
oligonucleotide against Epac2 blocks the GLP-1-potentiated insulin secretion
in the mouse pancreatic islets (6,7). Epac also acts as cAMP sensor, which
specifically interacted with ATP-sensitive K+ channel (ATP sensor), Piccolo
(Ca2+ sensor) and L-type voltage-dependent Ca2+ channels for insulin
exocytosis (6,8). The Ca2+ release by GLP-1 agonist, exendin-4, is blocked by
the overexpression of dominant negative Epac but increased by Epac selective
analogue, 8-pCPT |
List of Research Outputs |
Chu Y.S.J., Chung C.K.S., Lam A.K.M., Tam S., Chung S.K. and Chow B.K.C., Phenotypes Developed in Secretin Receptor-Null Mice Indicated a Role for Secretin in Regulating Renal Water Reabsorption, Molecular and Cellular Biology. 2007, 27: 2499-2511. |
Ho H.T.B., Ko C.B., Cheung A.K.H., Lam A.K.M., Tam S., Chung S.K. and Chung S.S.M., Generation and characterization of sodium-dicarboxylate cotransporter-deficient mice, Kidney International. 2007, 72: 63-71. |
Mak M.C., Lo A.C.Y., Lam A.K.M., Chung S.S.M. and Chung S.K., NFAT5 deficiency increases the severity of neuronal cell death in ischemic , 36th Annual Meeting of the Society for Neuroscience 2006. |
Researcher
: Lau KW |
List of Research Outputs |
Lau K.W., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma. Does it differentiate them?, The 4th Congress of the Federation of Asian-Oceanian Neuroscience societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 97-98 No. P-B37. |
Lau K.W., Lai S.W., So K.F. and Chang R.C.C., Impact of retinoic acid on
SH-SY5Y neuroblastoma to neurotoxins, Second International Symposiunm on
Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4,
2007, |
Researcher
: Lau TY |
List of Research Outputs |
Lau
T.Y. and Chiu
J., The possible role of cytokeratin |
Tipoe G.L., Leung T.M., Liong E.C., So H.S.H., Leung K.M., Lau T.Y., Tom W.M., Fung M.L., Fan S.T. and Nanji A.A., Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury, Histology and Histopathology. 2006, 21(11): 1157-1165. |
Researcher
: Lau WM |
List of Research Outputs |
Lau W.M., Tsao G.S.W., So K.F. and Yip H.K.F., Expression Of Telomerase
Reverse Transcriptase In Adult Goldfish Retina, J. Molecular Neuroscience.
|
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, Neuroscience Bulletin. 2007, 23: 131-136. |
Researcher
: Lau WM |
List of Research Outputs |
Lau W.M., Tsao G.S.W., So K.F. and Yip H.K.F., Expression Of Telomerase
Reverse Transcriptase In Adult Goldfish Retina, J. Molecular Neuroscience.
|
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, Neuroscience Bulletin. 2007, 23: 131-136. |
Researcher
: Lee DTW |
List of Research Outputs |
Chu Q., Lee D.T.W., Tsao G.S.W., Wang X. and Wong Y.C., S-allycysteine, a water-soluble garlic derivative, suppresses the growth of a human androgen-independent prostate cancer xenograft, CWR22R, under in vivo conditions, BJU International. 2006, 99: 925-932. |
Wong
Y.C., Chu Q., Lee D.T.W. and Wang X., S-allylcysteine (SAC), a garlic
derivative, suppresses the growth fo androgen-independent prostate cancer
xenograft under in vivo conditiion, AACR Special Conference in Cancer
Research: Innovations in Prostate Cancer Research, December 6-9, 2006, |
Researcher
: Leong VYL |
List of Research Outputs |
Ching Y.P., Leong V.Y.L., Lee M.F., Xu H., Jin D. and Ng I.O.L., P21-activated protein kinase is overexpressed in hepatocellular carcinoma and enhances cancer metastasis involving c-Jun NH2-terminal kinase activation and paxillin phosphorylation. , Cancer Research. 2007, 67: 3601-3608. |
Liu
M.H.F., Leong V.Y.L., Ng I.O.L. and Ching Y.P., The tumor suppressive
function of AMPK in hepatocellular carcinoma, Proceedings of the Annual
Meeting of American Association for Cancer Research, |
Researcher
: Leung KM |
List of Research Outputs |
Tipoe G.L., Leung T.M., Liong E.C., So H.S.H., Leung K.M., Lau T.Y., Tom W.M., Fung M.L., Fan S.T. and Nanji A.A., Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury, Histology and Histopathology. 2006, 21(11): 1157-1165. |
Researcher
: Leung TM |
List of Research Outputs |
Leung T.M., An in vivo study on the distinctive role of inducible and endothelial nitric oxide synthase in carbon tetrachloride-induced liver injury, 2006, 150 pages. |
Leung T.M., Liong E.C., Fung M.L., Lau T.Y.H., Nanji A.A. and Tipoe G.L., Inhibition of inducible nitric oxide synthase (iNOS) or administration of L-arginine reverses the induction of hypoxia-inducible factor (HIF-1a) in experimental liver fibrosis, AALSD Meeting, San Francisco USA. 2006. |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcholic fatty liver disease (NAFLD) not
requiring a high fat diet, Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcoholic fatty liver disease (NAFLD) not
requiring a high fat diet 1275 Suppl, Hepatology. 2006, 44(4): |
Tipoe
G.L., Leung T.M., Liong E.C., Fung M.L., Lau T. and Nanji A.A.,
Antioxidant and antiinflammatory effects of green tea polyphenols in carbon
tetrachloride induced liver fibrosis in mice, Hepatology. 2006, 44(4)
Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorate pathological changes, oxidative stress and
pro-inflammatory markrs in an animal model of non-alcoholic fatty liver
disease [NAFLD], Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.H.Y., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorated pathological changes, oxidative stress and
pro-inflammatory markers in an animal model of non-alcoholic fatty liver
disease (NAFLD) animal model 1063 Suppl 1, Hepatology . 2006, 44(4): |
Tipoe G.L., Leung T.M., Hung M.W. and Fung M.L., Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection, Cardiovascular & Hamatological Disorders Drug Targets. 2007, 7(2): 135-144. |
Tipoe G.L., Leung T.M., Hung M.W. and Fung M.L., Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection, Cardiovascular & Hematological Disorders - Drug Targets. 2007, 7(2): 135-144. |
Tipoe G.L., Leung T.M., Liong E.C., So H.S.H., Leung K.M., Lau T.Y., Tom W.M., Fung M.L., Fan S.T. and Nanji A.A., Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury, Histology and Histopathology. 2006, 21(11): 1157-1165. |
Tipoe
G.L., Leung T.M., Liong E.C., Fung M.L., Lau T.Y.H. and Nanji A.A.,
Lau and A.A. Nanji. Antioxidant and antiinflammatory effects of green tea
polyphenols in carbon tetrachloride induced liver fibrosis in mice, Hepatology.
2006, 44(4): |
Researcher
: Leung TM |
List of Research Outputs |
Leung T.M., An in vivo study on the distinctive role of inducible and endothelial nitric oxide synthase in carbon tetrachloride-induced liver injury, 2006, 150 pages. |
Leung T.M., Liong E.C., Fung M.L., Lau T.Y.H., Nanji A.A. and Tipoe G.L., Inhibition of inducible nitric oxide synthase (iNOS) or administration of L-arginine reverses the induction of hypoxia-inducible factor (HIF-1a) in experimental liver fibrosis, AALSD Meeting, San Francisco USA. 2006. |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcholic fatty liver disease (NAFLD) not
requiring a high fat diet, Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcoholic fatty liver disease (NAFLD) not
requiring a high fat diet 1275 Suppl, Hepatology. 2006, 44(4): |
Tipoe
G.L., Leung T.M., Liong E.C., Fung M.L., Lau T. and Nanji A.A.,
Antioxidant and antiinflammatory effects of green tea polyphenols in carbon
tetrachloride induced liver fibrosis in mice, Hepatology. 2006, 44(4)
Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorate pathological changes, oxidative stress and
pro-inflammatory markrs in an animal model of non-alcoholic fatty liver
disease [NAFLD], Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.H.Y., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorated pathological changes, oxidative stress and
pro-inflammatory markers in an animal model of non-alcoholic fatty liver
disease (NAFLD) animal model 1063 Suppl 1, Hepatology . 2006, 44(4): |
Tipoe G.L., Leung T.M., Hung M.W. and Fung M.L., Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection, Cardiovascular & Hamatological Disorders Drug Targets. 2007, 7(2): 135-144. |
Tipoe G.L., Leung T.M., Hung M.W. and Fung M.L., Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection, Cardiovascular & Hematological Disorders - Drug Targets. 2007, 7(2): 135-144. |
Tipoe G.L., Leung T.M., Liong E.C., So H.S.H., Leung K.M., Lau T.Y., Tom W.M., Fung M.L., Fan S.T. and Nanji A.A., Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury, Histology and Histopathology. 2006, 21(11): 1157-1165. |
Tipoe
G.L., Leung T.M., Liong E.C., Fung M.L., Lau T.Y.H. and Nanji A.A.,
Lau and A.A. Nanji. Antioxidant and antiinflammatory effects of green tea
polyphenols in carbon tetrachloride induced liver fibrosis in mice, Hepatology.
2006, 44(4): |
Researcher
: Leung WC |
List of Research Outputs |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 11th Reserach Postgraduate symposium 2006. |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 36th Annual Meeting of the Society for Neuroscience 2006. |
Researcher
: Leung WC |
List of Research Outputs |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 11th Reserach Postgraduate symposium 2006. |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 36th Annual Meeting of the Society for Neuroscience 2006. |
Researcher
: Li B |
List of Research Outputs |
Li B., Cheung P.Y., Wang X., Tsao G.S.W., Ling M.T., Wong Y.C. and Cheung A., Id-1 protects esophageal cancer cells from TNF-a-induced apoptosis through activation of P13K/AKT/NFkb signaling pathway, Proceedings of the Aerican Association for Cancer Research Annual Meeting, Los Angeles, U. S. A., April 14-18, 2007.. 2007, No. 5162. |
Researcher
: Li G |
List of Research Outputs |
Li
G. and Chiu
J., Activations of Stat1 and Erk are involbed in the resveratrol-induced
human esophageal cancer cell apoptosis, 2006 AACR International Conference
on Frontiers in Cancer Prevention Research, November 11-15, 2006, |
Researcher
: Li H |
List of Research Outputs |
Li H., Cell and gene therapies for diabetes - exploration of novel therapeutic approaches. 2006, 182 pages. |
Researcher
: Li SY |
List of Research Outputs |
Li S.Y., Tay D.K.C., Chan H.H.L. and So K.F., Changes of retinal functions following the induction of ocularhypertension in rats using argon laser photocoagulation, Clinical and Experimental Ophthalmology. 2006, 34: 575-583. |
Li S.Y., Functional changes and differenttial cell death of retinal ganglion cells after injury. 2007, 94 pages. |
Li S.Y., Chen B., Tay D.K.C., Chan H.H.L., Pu M.L. and So K.F., Melanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension model, Investigative Ophthalmology & Visual Science. 2006, 47(7): 2951-2958. |
Pu M., Chen B., Li S.Y., Tay D.K.C. and So K.F., A suprachiasmatic nucleus projecting retinal ganglion cell exhibits an unusually large dendritic field in the hamster, NeuroReport. 2006, 17(14): 1469-1472. |
Researcher
: Li X |
List of Research Outputs |
Zhang Z., Xie D., Li X., Wong Y.C., Xin D., Guan X.Y., Chua C.W., Leung S.C., Na Y. and Wang X., Significance of TWIST expression and its association with E-cadherin in bladder cancer., Human Pathology. 2007, 38: 598-606. |
Researcher
: Liang Y |
List of Research Outputs |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., So K.F. and Wu E.X., Chronic Injury Visualization of Regenerating Axons in Vivo in Hamster Optic Tract Transection Utilizing a 7 Tesla FMRI and a Nano Contrast Agent, Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
Ellis-Behnke R.G., Liang Y., You S.W., Tay D.K.C., Zhang S., Schneider G.E. and So K.F., Nano Neuro Knitting Nanotechnology For Hemostasis, Brain And Organ Repair, Inaugural Tissue Engineering (SuTEN), Australia, November 23, 2006. |
Ellis-Behnke
R.G., Liang Y., You S., Tay D.K.C., Zhang S., Wu W., So
K.F. and Schneider G.E., Nano
Neuro Knitting: Using Nanotechnology To Repair The CNS, NSTI Nanotech and
Bio Nano, |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., Wu W., Schneider G.E., Zhang S. and So K.F., Nano hemostat solution: Immediate hemostasis at the nanoscale, Nanomedicine: Nanotechnology, Biology and Medicine. 2006, 2(4): 207-215. |
Ellis-Behnke R.G., Liang Y., Wu W., You S.W., Schneider G. and So K.F., Nanotechnology and CNS repair, The 5th Asia-Pacific Symposium on Neural Regeneration, Shanghai, December 8-10, 2006, Shanghai, China. 2006, P15/72. |
Guo J., Zeng Y.S., Liang Y., Wang L., Su H. and Wu W., Cyclosporine affects the proliferation and differentiation of neural stem cells in culture, NeuroReport. 2007, 18(9): 863-868. |
Liang Y., Ellis-Behnke R.G., Tay D.K.C., You S., Schneider G.E. and So K.F., Creation of a more permissive environment using self-assembling peptide nanofiber scaffold in combination with chondroitinase ABC for brain lesion repair and functional return of vision, Society for Neuroscience. 2006, Program No. 720.6. |
Schneider G.E., Ellis-Behnke R.G., Liang Y., Lui Kau K.W.F., Tay D.K.C. and So K.F., Behavioral testing and analysis of the hamster visual system, Nature Protocols. 2006, 1(4): 1898-1901. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of Self-assembling Peptide Nanofiber Scaffold and Chondroitinase-ABC Appear to Create a More Permissive Environment in Optic Tract Brain Lesion Repair Resulting in Return of Vision, Annual Meeting of the Association for Vision and Research in Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of self-assembling peptide nanofiber scaffold and chondroitinase-ABC appear to create a more permissive environment in optic tract brain lesion repair resulting in return of vision, Association for Research in Vision and Ophthalmology, Ft Lauderdale USA, . 2007, Poster No. 3168. |
So K.F., Liang Y., You S., Tay D.K.C., Schneider G.E. and Ellis-Behnke R.G., Promotion of axonal growth by CNTF in a permissive environment of self-assembling peptide nanofiber scaffold for brain lesion repair and functional return of vision in adult hamsters, Society for Neuroscience. 2006, Program No. 522.14. |
Researcher
: Liao S |
List of Research Outputs |
Liao S., Chow P.H., Cheung M.P.L. and O W.S., Leptin in embryos sired by males without accessory sex glands, Society for Reproduction and Fertility Abstract Series No. 33 (ISSN 1476-3990). 2006, 33 No. O16. |
Researcher
: Ling MT |
Project Title: |
The regulation
and role of Id |
Investigator(s): |
Ling MT, Wong YC, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
09/2005 |
Abstract: |
Background: Prostate cancer remains the
most commonly diagnosed cancer in Western countries and recent reports
indicated that the incidence and mortality rate of prostate cancer are rising
significantly in Asian countries including |
Project Title: |
The role of EMI |
Investigator(s): |
Ling MT, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
02/2007 |
Abstract: |
Background: Prostate cancer is currently
the most commonly diagnosed male cancer in Western countries and recent
statistic data suggests that the incidence is increasing in Asian countries,
including |
List of Research Outputs |
Chu
Q., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Garlic derivatives inhibit
prostate cancer cell growth and invasion through restoration of E-cadherin
expression, 8th Asian Congress of Urology, |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-Beta1 sensitivity in prostate epithelial cells, Biology of the Cell. 2006, 98(9): 523-533. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-β1 sensitivity in prostate epithelial cells. , Biology of the Cell. 2006, 98: 523-533. |
Fung K.L., Cheung H.W., Wong H.L., Yuen H.F., Ling M.T., Chan K.W., Wong Y.C., Cheung A. and Wang X., MAD2 expression and its significance in mitotic checkpoint control in testicular germ cell tumour. , Biochimica et Biophysica Acta - Molecular Cell Research . 2007, 1773: 821-832. |
Fung K.L., Cheung H.W., Ling M.T., Cheung A., Wong Y.C. and Wang X., Role of MEK/ERK pathway in the MAD2-mediated cisplatin sensitivity in testicular germ cell tumour cells, British Journal of Cancer. 2006, 95: 475-484. |
Howard E.W., Chua C.W., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Potent suppression of distant metastasis by garlic compound S-allylmercaptocysteine in an in vivo androgen independent prostate cancer model, AACR Special Conference in Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006, San Francisco, U. S. A.. 2006, A1. |
Lee K.W., Poon R.T.P., Yuen P.W., Ling M.T., Wang X., Wong Y.C., Guan X.Y., Man K., Tang Z.Y. and Fan S.T., Regulation of angiogenesis by Id-1 through hypoxia-inducible factor-1 mediated vascular endothelial growth factor up-regulation in hepatocellular carcinoma, Clinical Cancer Research. 2006, 12(23): 6910-6919. |
Lee K.W., Poon R.T.P., Yuen P.W., Ling M.T., Kwok W.K., Wang X., Wong Y.C., Guan X.Y., Man K., Chau K.L. and Fan S.T., Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition, Clinical Cancer Research. 2006, 12(18): 5369-5376. |
Li B., Cheung P.Y., Wang X., Tsao G.S.W., Ling M.T., Wong Y.C. and Cheung A., Id-1 protects esophageal cancer cells from TNF-a-induced apoptosis through activation of P13K/AKT/NFkb signaling pathway, Proceedings of the Aerican Association for Cancer Research Annual Meeting, Los Angeles, U. S. A., April 14-18, 2007.. 2007, No. 5162. |
Wong
Y.C., Zhang X., Ling M.T. and Wang X., Inactivation of Id-1 gene
induces sensitivity of prostate cancer cells to chemotherapeutic drugs, 5th
International Symposium on Hormonal Carcinogenesis, |
Xu K., Ling M.T., Wang X. and Wong Y.C., Evidence of a novel biomarker, s1-Casein, a milk protein, in benign prostatic hyperplasia., Prostate cancer and Prostate Diseases. 2006, 9: 293-297. |
Researcher
: Liong EC |
List of Research Outputs |
Hwang I.S.S., Fung M.L., Liong E.C., Tipoe G.L. and Tang F., Age-related changes in adrenomedullin expression and hypoxia-inducible factor 1 activity in the rat lung and their responses to hypoxia, The Fourth International Huaxia Congress of Endocrinology. 2007. |
Hwang
I.S.S., Fung M.L., Liong E.C., Tipoe G.L. and Tang F., Age-related changes in
adrenomedullin expression and hypoxia-inducible factor-1 activity in the rat
lung and their responses to hypoxia., Journal of Gerontology. 2007, |
Hwang
I.S.S., Fung M.L., Liong E.C., Tipoe G.L. and Tang F., Age-related changes in
adrenomedullin expresssion and hypoxia-inducible factor-1 activity in the rat
lung and their responses to hypoxia, Journal of Gerontology:Biological
Sciences. 2007, |
Lam S.S.Y., Tipoe G.L., Tjong Y.W., Liong E.C. and Fung M.L., Intermittent hypoxia regulates the renin-angiotensin system in the rat carotid body, 11th Research Postgraduate Symposium HKU. 2006, 2:16. |
Lam
S.S.Y., Tipoe G.L., Liong E.C., Leung P.S. and Fung M.L., Up-regulation of a local
renin-angiotensin system in the rat carotid body during chronic intermittent
hypoxia, The Physiology Symposium (Hong Kong-Taiwan) |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcholic fatty liver disease (NAFLD) not
requiring a high fat diet, Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Leung T.M., Liong E.C., Fung M.L., Lau T. and Nanji A.A.,
Antioxidant and antiinflammatory effects of green tea polyphenols in carbon
tetrachloride induced liver fibrosis in mice, Hepatology. 2006, 44(4)
Suppl. 1: |
Tipoe
G.L., Ho M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorate pathological changes, oxidative stress and
pro-inflammatory markrs in an animal model of non-alcoholic fatty liver
disease [NAFLD], Hepatology. 2006, 44(4) Suppl. 1: |
Researcher
: Lo ACY |
List of Research Outputs |
Cheung
K.H.A., Lo A.C.Y., Chung S.S.M. and Chung S.K., Deletion of aldose reductase
gene protects against neuronal death and edema in retina after transient
ischemia by preventing oxidative stress, 11th Research Postgraduate
Symposium 2006, |
Hung
K.L., Lo A.C.Y., Lamb B.T.
and Chung S.K., Astrocytic
over-expression of endothelin-1 accelerates sensorimotor gating deficit,
prolonged habituation and spatial reference memory impairment in APP mutant
mice, 11th Research Postgraduate Symposium 2006, |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 11th Reserach Postgraduate symposium 2006. |
Leung W.C., Lo A.C.Y., Chung S.S.M. and Chung S.K., Endothelium specific endothelin-1 over-expression induces superoxide production and angiogenesis after middle cerebral artery occlusion, 36th Annual Meeting of the Society for Neuroscience 2006. |
Lo A.C.Y., Hung K.L., Cheung K.H.A., He Q., Chiu J., Chung S.S.M. and Chung S.K., Aldose reductase-deficient mice are protected from iron- and transferrin-related oxidative stress and cerebral ischemic injury, 36th Annual Meeting of the Society for Neuroscience 2006. |
Mak M.C., Lo A.C.Y., Lam A.K.M., Chung S.S.M. and Chung S.K., NFAT5 deficiency increases the severity of neuronal cell death in ischemic , 36th Annual Meeting of the Society for Neuroscience 2006. |
Researcher
: Lok CN |
List of Research Outputs |
Lok C.N., Ho C.M., Chen R., He Q., Yu W.Y., Sun H., Tam P.K.H. and Chiu J., Proteomic analysis of the mode of antibacterial action of silver nanoparticles, Gordon Research Conference, Metal in Medicine, Oxford, United Kingdom, July 9-14, 2006. |
Tian J., Wong K.K.Y., Ho C.M., Lok C.N., Yu W.Y., Che C.M., Chiu J. and Tam P.K.H., Topical delivery of silver nanoparticles promotes wound healing, ChemMedChem. 2007, 2: 129-136. |
Wong K.K.Y., Tian J., Ho C.M., Lok C.N., Che C.M., Chiu J. and Tam P.K.H., Topical delivery of silver nanoparticles reduces systemic inflammation of burn and promotes wound healing, Nanomedicine : nanotechnology, biology, and medicine. 2006, 2(4): 306. |
Researcher
: Lui Kau KWF |
List of Research Outputs |
Schneider G.E., Ellis-Behnke R.G., Liang Y., Lui Kau K.W.F., Tay D.K.C. and So K.F., Behavioral testing and analysis of the hamster visual system, Nature Protocols. 2006, 1(4): 1898-1901. |
Researcher
: Mak MC |
List of Research Outputs |
Mak M.C., Lo A.C.Y., Lam A.K.M., Chung S.S.M. and Chung S.K., NFAT5 deficiency increases the severity of neuronal cell death in ischemic , 36th Annual Meeting of the Society for Neuroscience 2006. |
Researcher
: Man CWY |
List of Research Outputs |
Man
C.W.Y., Doxsey S., Rosa J. and Tsao G.S.W., Id1 induces centrosome
abnormalities, disrupt microtubule integrity and stabilizes Aurora A in human
epithelial cells. , In: CSHL, |
Man C.W.Y., Li Po Chun Postgraduate Scholarship, 2007. |
Researcher
: Millet JKG |
List of Research Outputs |
Kien F.S., Millet J.K.G., Teoh K.T., Siu Y.L. and Nal-Rogier B.T.M., Identification Of Cellular Factors Which Regulate Early And/or Late Stages of Sars Cov Replication Cycle., Scientific conference of the 38th Board of Institutes Pasteur Directors, National Institute of Hygiene and Epidemiology. . 2006. |
Nal-Rogier
B.T.M., Kien F.S., Siu Y.L., Millet J.K.G., Teoh K.T. and Tse K.S., Tracking Virus-Host Cell
Interactions: Example of the SARS coronavirus. , 1st RESPARI Workshop. |
Researcher
: Niu C |
List of Research Outputs |
Niu C. and Yip H.K.F., Neuronal Injury Affects Telomerase Activity In The Adult Rat., The 4th Congress Of Federation Of Asian-oceanian Neuroscience Societies (faons) & Annual Meeting Of The Hong Kong Society Of Neurosciences.. 2006. |
Researcher
: O WS |
Project Title: |
LEPTIN AND HUMAN SPERM FUNCTION |
Investigator(s): |
O WS, Yeung WSB, Li HWR |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
12/2005 |
Abstract: |
1. To demonstrate the expression pattern of different leptin receptor isoforms in human spermatozoa.2. To confirm the correlation of leptin concentration in seminal plasma and leptin receptor expression in spermatozoa with semen parameters (sperm concentration, motility, morphology, velocity curve linear, velocity average path and velocity straight line).3. To study the correlation of leptin concentration in seminal plasma and leptin receptor expression in spermatozoa with capacitation and acrosome reaction.4. To study the correlation of leptin concentration in seminal plasma and leptin receptor expression in spermatozoa with fertilisation outcome. |
Project Title: |
In vitro maturation - Mitochondria and oocyte competence |
Investigator(s): |
O WS, Yeung WSB |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
09/2006 |
Abstract: |
Objectives:(1) To establish a preantral follicle culture model system using rat oocytes;(2) To investigate the qunatitiy, function and distribution of mitochondria in oocytes matured in vitro; (3) To study the correlation of the mitochondria copies, functions and distribution in oocytes with fertilization outcome.BackgroundIt is evident from clinical IVF outcome that each embryo has a unique developmental potential. Current investigations suggested that physiological factors during oocyte development can have dowstream consequences for development (Cotichhio et al., 2004). Mitochondria dysfunctions or abnormalities in the oocyte have been found to be a critical determinant of embryo developmental competence (van Blerkom, 2004). The accumulation of mitochondria is a feature of oocyte maturation (Chen et al., 1995). During oogenesis, the mitochondrial copy number in primordial oocytes is |
List of Research Outputs |
Chan Y.F., O W.S. and Tang F., Testicular adrenomedullin and endothelin interaction, Society for Reproduction and Fertility Abstract Series No. 33 (ISSN 1476-3990). 2006, 33 No.P40. |
Huang Y., Wong C.L., Ho V.W.K., Poon H.K., O W.S. and Chow P.H., Identification of growth-differentiation factor 8 (GDF-8) in endometrium of the golden hamster, The FASEB Journal. 2007, 21(5): A223 No. 480.3. |
Li Y.Y., Hwang I.S.S., O W.S. and Tang F., Adrenomedullin peptide: gene expression of adrenomedullin, its receptors and receptor activiity modifying proteins, and receptor binding in rat testis-actions on testosterone secretion, Biology of Reproduction. 2006, 75: 183-188. |
Li Y.Y., O W.S. and Tang F., Ageing and adrenomedullin in the female reproductive system of the rat, Hong Kong Medical Journal. 2006, 12(6): 166 No. P204. |
Liao S., Chow P.H., Cheung M.P.L. and O W.S., Leptin in embryos sired by males without accessory sex glands, Society for Reproduction and Fertility Abstract Series No. 33 (ISSN 1476-3990). 2006, 33 No. O16. |
Man S.Y., Hwang I.S.S., Li Y.Y., O W.S., Sheng H.P. and Tang F., Differential regulation of adrenomedullin gene expression in the fundic and pyloric regions of the rat stomach during acute and chronic starvation, Neuropeptides. 2007, 41: 177-187. |
O W.S., Li Y.Y. and Tang F., Adrenomedullin and its receptors in the testis, Society for Reproduction and Fertility Abstract Series No. 33 (ISSN 1476-3990). 2006, 33 No.P41: 52-53. |
Tang F., Li Y.Y. and O W.S., Ageing and adrenomedullin in the male reproductive system of the rat, Hong Kong Medical Journal. 2006, 12(6): 165 No. P203. |
Tang F., Li Y.Y. and O W.S., Ageing and adrenomedullin in the male reproductive system, Society for Reproduction and Fertility Abstract Series No. 33 (ISSN 1476-3990). 2006, 33 No. P39: 52. |
Wong C.L., Lee K.H., Lo K.M., Chan O.C., Goggins W., O W.S. and Chow P.H., Ablation of paternal accessory sex glands imparts physical and behavioural abnormalities to the progeny: An in vivo study in the golden hamster, Theriogenology. 2007, 68: 654-662. |
Researcher
: Qiu G |
List of Research Outputs |
Lau W.M., Qiu G., Helmeste D.M., Lee T.M.C., Tang S.W. and So K.F., Reversal of corticosterone-induced subventricular zone cell proliferation decrease by paroxetine, Restorative Neurology and Neuroscience. 2007, 25: 17-23. |
Lau W.M., Yau S.Y., Qiu G., Helmeste D.M., Lee T.M.C., Tang S.W. and So K.F., Selective serotonin reuptake inhibitor treatment diminishes inhibition of masculine sexual behavior caused by corticosterone, Thd 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 120 No. P-C22. |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, Neuroscience Bulletin. 2007, 23: 131-136. |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, The 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 84 No. P-B05. |
Researcher
: Qiu G |
List of Research Outputs |
Lau W.M., Qiu G., Helmeste D.M., Lee T.M.C., Tang S.W. and So K.F., Reversal of corticosterone-induced subventricular zone cell proliferation decrease by paroxetine, Restorative Neurology and Neuroscience. 2007, 25: 17-23. |
Lau W.M., Yau S.Y., Qiu G., Helmeste D.M., Lee T.M.C., Tang S.W. and So K.F., Selective serotonin reuptake inhibitor treatment diminishes inhibition of masculine sexual behavior caused by corticosterone, Thd 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 120 No. P-C22. |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, Neuroscience Bulletin. 2007, 23: 131-136. |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, The 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 84 No. P-B05. |
Researcher
: Samaranayake NA |
List of Research Outputs |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, Neuroscience Bulletin. 2007, 23: 131-136. |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, The 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 84 No. P-B05. |
Researcher
: Schneider GE |
List of Research Outputs |
Ellis-Behnke
R.G., Liang Y., You S., Tay D.K.C., Zhang S., Wu W., So
K.F. and Schneider G.E.,
Nano Neuro Knitting: Using Nanotechnology To Repair The CNS, NSTI Nanotech
and Bio Nano, |
Ellis-Behnke R.G., So K.F. and Schneider G.E., The 4 P's of CNS regeneration: A framework for approaching the repair of neural trauma using nanotechnology and combination therapies, Society for Neuroscience. 2006, Program No. 228.23. |
Liang Y., Ellis-Behnke R.G., Tay D.K.C., You S., Schneider G.E. and So K.F., Creation of a more permissive environment using self-assembling peptide nanofiber scaffold in combination with chondroitinase ABC for brain lesion repair and functional return of vision, Society for Neuroscience. 2006, Program No. 720.6. |
So K.F., Liang Y., You S., Tay D.K.C., Schneider G.E. and Ellis-Behnke R.G., Promotion of axonal growth by CNTF in a permissive environment of self-assembling peptide nanofiber scaffold for brain lesion repair and functional return of vision in adult hamsters, Society for Neuroscience. 2006, Program No. 522.14. |
Researcher
: So KF |
Project Title: |
Neuroprotection in steroid therapy: an animal model |
Investigator(s): |
So KF, Lee TMC, Tang SW |
Department: |
Anatomy |
Source(s) of Funding: |
Research Fund for the Control of Infectious Diseases - Full Grants |
Start Date: |
01/2005 |
Abstract: |
To investigate the effect of Lithium and paroxetine in arresting hippocampal damages associated with high dosage of steroid through quantitative neuro-anatomical and immunohistochemical methods. |
Project Title: |
The role of KhcU on the development and intracellular transportation of rod bipolar cell of mice retina |
Investigator(s): |
So KF, Huang J |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
10/2005 |
Completion Date: |
09/2006 |
Abstract: |
In this study, we make use of the previously created Pcp2-IRES-Cre mouse line and knock out the ubiquitously expressed kinesin heavy chain (KhcU) specifically in rod bipolar cells. We aim to investigate: 1) the retinal phenotypic and developmental changes after KhcU knockout in rod bipolar; 2) the alterations of intracellular transportation in the absence of KhcU, the MyosinVa-mediated processes will be specially noted. |
Project Title: |
Hemostasis and Hong Kong: The future hub of Nanomedicine |
Investigator(s): |
So KF, Ellis-Behnke RG, Liang Y, |
Department: |
Anatomy |
Source(s) of Funding: |
Innovation and Technology Support Programme |
Start Date: |
09/2006 |
Abstract: |
In order to progress the work to humans we need to perform four additional experiements: 1. Identify the mechanism of action 2. Perform large animal trials in pigs with a liver laceration model 3. Determine the best way to measure the quality of the material during manufacturing 4. Follow up the breakdown of the material by radio labeling the material in the brain and liver |
Project Title: |
Nanobiomedical manufacturing: the workforce of tomorrow |
Investigator(s): |
So KF, Ellis-Behnke RG, |
Department: |
Anatomy |
Source(s) of Funding: |
Innovation and Technology Support Programme |
Start Date: |
09/2006 |
Abstract: |
The goal is to not only understand the variation in the manufacturing process, but to understand how to remove that variation. At the same time the process needs to be optimized for cost, purity and the quantities that will be needed for use in humans. |
Project Title: |
The role of
endothelin |
Investigator(s): |
So KF, Chung SK |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
03/2007 |
Abstract: |
Glaucoma is an ocular degenerative
disease and characterized by progressive damage of optic nerve fibers and
retinal ganglion cells (RGC), resulting in progressive visual field loss and
excavation of the optic nerve head. As a leading cause of blindness
worldwide, glaucoma continues to be a clinical problem. Optic nerve damage in
most forms of glaucoma is commonly associated with increased intraocular
pressure (IOP) caused by impairment in the normal aqueous humor outflow
pathway. Yet, increased IOP may not be the sole risk factor for glaucomatous
disease, and because the eye is a highly perfused organ, the vascular system
may also play a key role in disease progression. Indeed, death of RGC is
thought to be initiated after ischemia resulting from a prolonged
vasoconstriction and/or vasospasms from abnormal auto-regulation of the
retinal microcirculation. Recent experimental evidence suggests that
endothelin-1 (ET-1), a potent vasoconstrictor, may be an important
contributor to glaucoma pathophysiology. Studies indicate that perfusion
instability, resulted from a disturbed auto-regulation in the context of a
general vascular dysregulation, might contribute to glaucoma. Indeed, ET-1
levels in the aqueous humor and plasma are higher in patients with primary
open-angle glaucoma. Both vascular (endothelial) and glial (astrocytic) ET-1
are involved in the regulation of retinal micro-circulation; yet, the
involvement of these two components in pathophysiology of glaucoma
retinopathy is poorly understood. Transient ischemia-related diseases, such
as central retinal artery occlusion, angle-closure glaucoma and carotid
artery disease, also affect vision. Various methods, such as raising the IOP
through cannulation of the eye and ligation of the central retinal artery
together with the optic nerve, have been used to create a model of retinal
ischemia-reperfusion, but they have drawbacks and limitations. In light of
this, we decided to investigate the contribution of vascular and glial ET-1
to the pathogenesis of glaucoma and retinal ischemia-reperfusion using animal
models. As validation and interpretation of the published data on human
studies are difficult, animal systems may provide insights into exploring the
underlying molecular mechanisms of development of glaucoma. Dogs and rats
have provided valuable information on the pathogenesis of experimental
glaucoma in the form of ocular hypertension, but they are poorly suited for
these studies using modern methods of genetic manipulations. Building on our
longstanding expertise in studies using transgenic mice, we propose to use
transgenic mouse models to study the involvement of ET |
List of Research Outputs |
Chan B.P., Hui T.Y., Chan C.M., So K.F., Lu W., Cheung K.M.C., Salomatina E. and Yaroslavsky A., Photochemical cross-linking for collagen-based scaffolds: a study on optical properties, mechanical properties, stability, and hematocompatibility, Tissue Engineering. 2007, 13(1): 73-85. |
Chan
C.M., So K.F. and Chan B.P., Photochemically crosslinked
collagen microspheres for controlled drug release, Proceedings on the
Conference on Biomedical Engineering, BME2006. |
Chan H.C., Chang R.C.C., Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y. and So K.F., Neuroprotective effects of Lycium barbarum Lynn on protecting retinal ganglion cells in an ocular hypertension model of glaucoma, Experimental Neurology. 2006, 203: 269-273. |
Chen B., So K.F., Yu E. and Tay D.K.C., Expression of nicotinamide adenine dinucleotide phosphate-diaphorase in the retina of postnatal golden hamsters deprived of light stimulation, Neuroscience Letters. 2006, 405: 74-78. |
Chiu K., Ji J., Yu M.S., So K.F. and Chang R.C.C., Activation of microglia/macrophages determines the fate of retinal ganglion cell survival in rat chronic ocular hypertension model, Neurosignals. 2006, 15: 139. |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., So K.F. and Wu E.X., Chronic Injury Visualization of Regenerating Axons in Vivo in Hamster Optic Tract Transection Utilizing a 7 Tesla FMRI and a Nano Contrast Agent, Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
Ellis-Behnke R.G., So K.F. and Zhang S., Molecular repair of the brain using self-assembling peptides., Chimica Oggi. 2006, 24: 12-14. |
Ellis-Behnke R.G., So K.F. and Zhang S., Molecular repair of the brain using self-assembling peptides, Chemistry Today. 2006, 24(4): 42-43. |
Ellis-Behnke R.G., Teather L.A. and So K.F., Molecular restoration of the body: nano neuro knitting for brain repair, British Anti-Ageing Medical Journal. 2006, 4: 35-37. |
Ellis-Behnke R.G., Liang Y., You S.W., Tay D.K.C., Zhang S., Schneider G.E. and So K.F., Nano Neuro Knitting Nanotechnology For Hemostasis, Brain And Organ Repair, Inaugural Tissue Engineering (SuTEN), Australia, November 23, 2006. |
Ellis-Behnke
R.G., Liang Y., You S., Tay D.K.C., Zhang S., Wu W., So K.F. and Schneider G.E., Nano Neuro Knitting:
Using Nanotechnology To Repair The CNS, NSTI Nanotech and Bio Nano, |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., Wu W., Schneider G.E., Zhang S. and So K.F., Nano hemostat solution: Immediate hemostasis at the nanoscale, Nanomedicine: Nanotechnology, Biology and Medicine. 2006, 2(4): 207-215. |
Ellis-Behnke R.G., Liang Y., Wu W., You S.W., Schneider G. and So K.F., Nanotechnology and CNS repair, The 5th Asia-Pacific Symposium on Neural Regeneration, Shanghai, December 8-10, 2006, Shanghai, China. 2006, P15/72. |
Ellis-Behnke R.G., So K.F. and Schneider G.E., The 4 P's of CNS regeneration: A framework for approaching the repair of neural trauma using nanotechnology and combination therapies, Society for Neuroscience. 2006, Program No. 228.23. |
Fu Q., Wu W., Hu B., Chan S.Y.M., Shao Z., Pepinsky R.B., Mi S. and So K.F., LINGO-1 Antagonists protects retinal ganglion cells in a chronic hypertensive model of glaucoma, the 29th Annual Meeting of the Japan Neuroscience Society, Kyoto, Japan, July 19-21, 2006. S119 No. PS1P-E073. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Alkaline extract of lycium barbarum protects against beta-amyloid peptide neurotoxicity in rat cortical neurons by activation of AKT, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 126-127 No. P-C36. |
Ho
Y.S., Yu M.S., So K.F., Yuen W.H. and Chang R.C.C., Attenuation of unfolded
protein responses by reducing stress: an example of neuroprotective effect of
Lycium barbarum, 2006 Hong Kong-Macau Postgraduate Symposium on
Chinese Medicine, August 17, 2006, |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Characterizing the Neuroprotective Effects of Alkaline Extract of Lycium Barbarumon b-amyloid Peptide Neurotoxicity , Brain Research . 2007, 1158: 123-134. |
Ho Y.S., Yu M.S., Lai S.W., So K.F. and Chang R.C.C., Glycoconjugates from anti-aging Lycium Barbarum protect primary cortical neurons from beta-amyloid neurotoxicity, Second International Symposium on Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4, 2007 Hong Kong. 2007, 53 P2. |
Ho Y.S., Yu M.S., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity, Society for Neuroscience. 2006, Program No. 826.10. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Neuroprotective effects of anti-aging Lycium barbarum by a novel extraction method, 2006 World Congress on Chinese Medicine: Charting the Course of Development, Hong Kong, November 23-25, 2006. 247. |
Ip K.C., Chiu K., Yuen W.H., Zee S.S.Y., Chang R.C.C. and So K.F., Neuroprotective effect of Lycium barbarum in rat chronic ocular hypertension model via immunomodulation of macrophages/microglia, Neurosignals. 2006, 15: 145. |
Ji B., Li M., Wu W., Yick L.W., Lee X., Shao Z., Wang J., So K.F., McCoy J.M., Pepinsky R.B., Mi S. and Relton J.K., LINGO-1 antagonist promotes functional recovery and axonal sprouting after spinal cord injuiry, Molecular and Cellular Neuroscience. 2006, 33: 311-320. |
Lai S.W., So K.F. and Chang R.C.C., Aggregation/collapse of endoplasmic retiulum induced by Ab subsequently undergo autophagy, Second International Symposium on Healthy Aging; Meeting the Challenges of an Aging Population, March 3-4, 2007, Hong Kong. 2007, 53 P4. |
Lai S.W., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Neuroprotective effects of the gandoderma lucidum aqueous extract against beta-amyloid peptide-induced neurotoxicity, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006 . 2006, 97 No. P-B36. |
Lai S.W., Yu M.S., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Potential neuroprotective agent from botanical extract: An experience of using Verbena officinalisagainst b-amyloid peptide neurotoxicity, Neurosignals. 2006, 15: 146. |
Lai S.W., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., The aqueous extract from anti-aging Ganoderma lucidum inhibits beta-amyloid peptide-induced neurotoxicity, Society for Neuroscience. 2006, Program No. 826.11. |
Lau K.W., Lai C.S., Yuen W.H., So K.F. and Chang R.C.C., Differential effects of parkinsonism mimetics on SH-SY5Y neuroblastoma , Society for Neuroscience. 2006, Program No. 824.19. |
Lau K.W., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma. Does it differentiate them?, The 4th Congress of the Federation of Asian-Oceanian Neuroscience societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 97-98 No. P-B37. |
Lau
K.W., Lai S.W., So K.F. and Chang R.C.C., Impact of retinoic acid on
SH-SY5Y neuroblastoma to neurotoxins, Second International Symposiunm on
Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4,
2007, |
Lau
W.M., Tsao G.S.W., So K.F. and Yip H.K.F., Expression Of Telomerase
Reverse Transcriptase In Adult Goldfish Retina, J. Molecular Neuroscience.
|
Lau W.M., Qiu G., Helmeste D.M., Lee T.M.C., Tang S.W. and So K.F., Reversal of corticosterone-induced subventricular zone cell proliferation decrease by paroxetine, Restorative Neurology and Neuroscience. 2007, 25: 17-23. |
Lau W.M., Yau S.Y., Qiu G., Helmeste D.M., Lee T.M.C., Tang S.W. and So K.F., Selective serotonin reuptake inhibitor treatment diminishes inhibition of masculine sexual behavior caused by corticosterone, Thd 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 120 No. P-C22. |
Lee
W.S., Shen J., So K.F. and Tong Y., NITRIC OXIDE STIMULATES
CAVEOLIN-1 EXPRESSION AND INTERACTION OF CAVEOLIN-1 AND NITRIC OXIDE SYNTHASE
REGULATES NITRIC OXIDE PRODUCTION IN HUMAN SK-N-MC NEURAL CELLS UNDER HYPOXIA
CONDITION, Second International Symposium on Healthy Aging: “Meeting the
Challenges of an Aging Population”. |
Li S.Y., Tay D.K.C., Chan H.H.L. and So K.F., Changes of retinal functions following the induction of ocularhypertension in rats using argon laser photocoagulation, Clinical and Experimental Ophthalmology. 2006, 34: 575-583. |
Li S.Y., Chen B., Tay D.K.C., Chan H.H.L., Pu M.L. and So K.F., Melanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension model, Investigative Ophthalmology & Visual Science. 2006, 47(7): 2951-2958. |
Liang Y., Ellis-Behnke R.G., Tay D.K.C., You S., Schneider G.E. and So K.F., Creation of a more permissive environment using self-assembling peptide nanofiber scaffold in combination with chondroitinase ABC for brain lesion repair and functional return of vision, Society for Neuroscience. 2006, Program No. 720.6. |
Lukehurst S.S., King C.E., Beazley L.D., Tay D.K.C., So K.F. and Rodger J., Graded ephrin-A2 expression in the developing hamster superior colliculus, Experimental Brain Research. 2006, 173: 546-552. |
Lukehurst S.S., King C.E., Beazley L.D., Tay D.K.C., So K.F. and Rodger J., Graded ephrin-A2 expression in the developing hamster superior colliculus, The Sixteenth Annual Combined Biological Sciences Meeting, Australia, August 18, 2006. 2006, 37 No. 08. |
Nan Y., Xiao C.X., Zhang Y., Chen B.Y., Yu E.H., Ellis-Behnke R.G., So K.F., Lewis G.P., Fisher S.K. and Pu M., Visual response properties of cat retinal ganglion cells after retinal detachment, Association for Research in Vision and Ophthalmology, Ft Lauderdale USA. 2007, Program No. 5754 No. B73. |
Pu M., Chen B., Li S.Y., Tay D.K.C. and So K.F., A suprachiasmatic nucleus projecting retinal ganglion cell exhibits an unusually large dendritic field in the hamster, NeuroReport. 2006, 17(14): 1469-1472. |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, Neuroscience Bulletin. 2007, 23: 131-136. |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, The 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 84 No. P-B05. |
Schneider G.E., Ellis-Behnke R.G., Liang Y., Lui Kau K.W.F., Tay D.K.C. and So K.F., Behavioral testing and analysis of the hamster visual system, Nature Protocols. 2006, 1(4): 1898-1901. |
So K.F., Axonal regeneration in the central nervous system, Neuro-signals . 2006, 15(3): 113 No. PL-1/2. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of Self-assembling Peptide Nanofiber Scaffold and Chondroitinase-ABC Appear to Create a More Permissive Environment in Optic Tract Brain Lesion Repair Resulting in Return of Vision, Annual Meeting of the Association for Vision and Research in Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of self-assembling peptide nanofiber scaffold and chondroitinase-ABC appear to create a more permissive environment in optic tract brain lesion repair resulting in return of vision, Association for Research in Vision and Ophthalmology, Ft Lauderdale USA, . 2007, Poster No. 3168. |
So K.F., Depression and sexual dysfunction: a matter of neurogenesis and neural connections?, Acta Biophysica Sinica. 2006, 22 suppl.2: 7. |
So K.F., Ho L.S.H., Tay D.K.C. and Lee T.M.C., Light delays synaptic deafferentation and potentiates the survival of axotomized retinal ganglion cells. , Neuroscience Letters. 2006, 395: 255-260. |
So K.F., Chan H.C., Chang R.C.C., Chan S.Y.M., Yuen W.H. and Zee S.S.Y., Modulation of microglia by Chinese herbal medicine Lycium barbarum and neuroprotection of retinal ganglion cells in experimental glaucoma, 4th Asian-Pacific International Congress of Anatomists, September 7-10, 2005, Kusadasi, Turkey. 2006, 37. |
So
K.F. and Chang
R.C.C., Molecular basis of neuroprotection in glaucoma and Alzheimer's
disease using Gouqizi, 2006 World Congress on Chinese Medicine: Charting
the Course of Development, November 23-26, 2006, |
So K.F. and Chang R.C.C., Molecular basis of neuroprotection using anti-ageing chinese herb, 6th International Symposium on Frontiers in Life Sciences, Qingdao, China, September 20-23, 2006. |
So
K.F. and Ellis-Behnke
R.G., Nano neuro knitting: peptide nanofiber scaffold for brain repair
and axon regeneration with functional return of vision, International
Symposium on Applied Neuroscience: Recovering from Brain Trauma, May 4-5,
2007, |
So K.F. and Ellis-Behnke R.G., Nanomedicine for CNS regeneration, wound healing, and instant hemostasis, 第二届全国組织工程干細胞与神经再生學術会議論文集. 2006, 4. |
So K.F., Liang Y., You S., Tay D.K.C., Schneider G.E. and Ellis-Behnke R.G., Promotion of axonal growth by CNTF in a permissive environment of self-assembling peptide nanofiber scaffold for brain lesion repair and functional return of vision in adult hamsters, Society for Neuroscience. 2006, Program No. 522.14. |
So
K.F., Protection of retinal ganglion cells in
glaucoma, The 5th Asia-pacific symposium on Neural Regeneration, December
8-10, 2006, shanghai, |
Tang H.C., Leung F.P., Huang Y., Feletou M., So K.F., Man R.Y.K. and Vanhoutte P.M.G.R., Calcium and rea tive oxygen species increase in endothelial cells in response to releasers of endothelium-derived contracting factor, British Journal of Pharmacology. 2007, 151(1): 15-23. |
Yau S.Y., Lau W.M., Tang S.W., Lee T.M.C. and So K.F., Counteractive effect of voluntary exercise on depression-like behavior of corticoseterone treated rats. , The University of Hong Kong Research postgraduate Symposium. 2007. |
Yeung
S.C., Chiu K., So K.F. and Chang R.C.C., The effects of
intravitreal injection of IL-10 on the survival of retinal ganglion cells in
the rat glaucoma model, The 5th Asia-Pacific symposium on Neural
Regeneration, |
Yik
S.Y., Ho Y.S., Lai S.W., So K.F. and Chang R.C.C., Significance of dsRNA
produced by viral infection in neurodegeneration, Second International
Symposium on Healthy Aging: Meeting the Challenges of an Aging Population,
March 3-4, 2007, |
Yu
M.S., So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., A new polysaccharide from
nerium indicum elicits neuroprotection against beta-amyloid peptides-induced
apoptosis, Second International Symposium on Healthy Aging: Meeting the
challenges of an Aging Population, March 3-4, 2007, |
Yu M.S., Lai S.W., Suen K.A., So K.F., Hugon J. and Chang R.C.C., Absence of unfolded protein responses in extracellular beta-amyloid peptide-induced neuronal apoptosis, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 129 No. P-C41. |
Yu M.S., Ho Y.S., So K.F., Yuen W.H. and Chang R.C.C., Cytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulum, Neurosignals. 2006, 15: 145. |
Yu M.S., Lai S.W., Suen K.C., Kwok N.S., So K.F., Hugon J. and Chang R.C.C., Extracellular accumulation of beta-amyloid peptides induces apoptosis in cultured neurons via a mechanism independent of unfolded protein responses, Neurosignals. 2006, 15: 141. |
Yu M.S., Wong Y.Y., So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., New polysaccharide from Nerium indicum protects neurons via stress kinase signaling pathway, Brain Research. 2007, 1153: 221-230. |
Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Protein kinases as technological platforms to screen neuroprotective agents from chinese medicine, Neurosignals. 2006, 15: 133. |
Yu M.S., Yuen W.H., So K.F. and Chang R.C.C., Significance of neuroprotective polysaccharide from the flowers of Nerium indicum in beta-amyloid peptides neurotoxicity, Society for Neuroscience. 2006, Program No. 826.9. |
Yuan Q.J., Scott D.E., So K.F. and Wu W., A subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats, Brain Research. 2007, 1159: 18-27. |
Yuan Q.J., Scott D.E., So K.F. and Wu W., The response of magnocellular neurons of the hypothalamo-neurohyphyseal system to hypophysectomy, nitric oxide synthase expression as well as survival and regeneration in developing vs. adult rats, Brain Research. 2006, 1113: 45-53. |
Researcher
: Su H |
List of Research Outputs |
Guo J., Zeng Y.S., Liang Y., Wang L., Su H. and Wu W., Cyclosporine affects the proliferation and differentiation of neural stem cells in culture, NeuroReport. 2007, 18(9): 863-868. |
Su H., Chu T.H. and Wu W., Lithium enhances proliferation and neuronal differentiation of neural progenitor cells in vitro and after transplantation into the adult rat spinal cord, Experimental Neurology. 2007, 206: 296-307. |
Researcher
: Suen KC |
List of Research Outputs |
Suen
K.C. and Chang
R.C.C., Increment of sleeping time does not implicate enhancement on
memory and learning in adolescents (Neuroscientist - Teacher Partner Award), Society
for |
Yu M.S., Lai S.W., Suen K.C., Kwok N.S., So K.F., Hugon J. and Chang R.C.C., Extracellular accumulation of beta-amyloid peptides induces apoptosis in cultured neurons via a mechanism independent of unfolded protein responses, Neurosignals. 2006, 15: 141. |
Researcher
: Tang SW |
List of Research Outputs |
Qiu G., Helmeste D.M., Samaranayake N.A., Lau W.M., Lee T.M.C., Tang S.W. and So K.F., Modulation by paroxetine of suppressive effect of corticosterone on adult hippocampal cell proliferation, The 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 84 No. P-B05. |
List of Research Outputs |
Chen B., So K.F., Yu E. and Tay D.K.C., Expression of nicotinamide adenine dinucleotide phosphate-diaphorase in the retina of postnatal golden hamsters deprived of light stimulation, Neuroscience Letters. 2006, 405: 74-78. |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., So K.F. and Wu E.X., Chronic Injury Visualization of Regenerating Axons in Vivo in Hamster Optic Tract Transection Utilizing a 7 Tesla FMRI and a Nano Contrast Agent, Annual Meeting of the Association for Research in Vision and Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
Ellis-Behnke R.G., Liang Y., You S.W., Tay D.K.C., Zhang S., Schneider G.E. and So K.F., Nano Neuro Knitting Nanotechnology For Hemostasis, Brain And Organ Repair, Inaugural Tissue Engineering (SuTEN), Australia, November 23, 2006. |
Ellis-Behnke
R.G., Liang Y., You S., Tay D.K.C., Zhang S., Wu W., So
K.F. and Schneider G.E., Nano
Neuro Knitting: Using Nanotechnology To Repair The CNS, NSTI Nanotech and
Bio Nano, |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., Wu W., Schneider G.E., Zhang S. and So K.F., Nano hemostat solution: Immediate hemostasis at the nanoscale, Nanomedicine: Nanotechnology, Biology and Medicine. 2006, 2(4): 207-215. |
Li S.Y., Tay D.K.C., Chan H.H.L. and So K.F., Changes of retinal functions following the induction of ocularhypertension in rats using argon laser photocoagulation, Clinical and Experimental Ophthalmology. 2006, 34: 575-583. |
Li S.Y., Chen B., Tay D.K.C., Chan H.H.L., Pu M.L. and So K.F., Melanopsin-expressing retinal ganglion cells are more injury-resistant in a chronic ocular hypertension model, Investigative Ophthalmology & Visual Science. 2006, 47(7): 2951-2958. |
Liang Y., Ellis-Behnke R.G., Tay D.K.C., You S., Schneider G.E. and So K.F., Creation of a more permissive environment using self-assembling peptide nanofiber scaffold in combination with chondroitinase ABC for brain lesion repair and functional return of vision, Society for Neuroscience. 2006, Program No. 720.6. |
Lukehurst S.S., King C.E., Beazley L.D., Tay D.K.C., So K.F. and Rodger J., Graded ephrin-A2 expression in the developing hamster superior colliculus, Experimental Brain Research. 2006, 173: 546-552. |
Lukehurst S.S., King C.E., Beazley L.D., Tay D.K.C., So K.F. and Rodger J., Graded ephrin-A2 expression in the developing hamster superior colliculus, The Sixteenth Annual Combined Biological Sciences Meeting, Australia, August 18, 2006. 2006, 37 No. 08. |
Pu M., Chen B., Li S.Y., Tay D.K.C. and So K.F., A suprachiasmatic nucleus projecting retinal ganglion cell exhibits an unusually large dendritic field in the hamster, NeuroReport. 2006, 17(14): 1469-1472. |
Schneider G.E., Ellis-Behnke R.G., Liang Y., Lui Kau K.W.F., Tay D.K.C. and So K.F., Behavioral testing and analysis of the hamster visual system, Nature Protocols. 2006, 1(4): 1898-1901. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of Self-assembling Peptide Nanofiber Scaffold and Chondroitinase-ABC Appear to Create a More Permissive Environment in Optic Tract Brain Lesion Repair Resulting in Return of Vision, Annual Meeting of the Association for Vision and Research in Ophthalmology (ARVO), Ft Lauderdale USA. 2007. |
So K.F., Liang Y., Tay D.K.C. and Ellis-Behnke R.G., Combinations of self-assembling peptide nanofiber scaffold and chondroitinase-ABC appear to create a more permissive environment in optic tract brain lesion repair resulting in return of vision, Association for Research in Vision and Ophthalmology, Ft Lauderdale USA, . 2007, Poster No. 3168. |
So K.F., Ho L.S.H., Tay D.K.C. and Lee T.M.C., Light delays synaptic deafferentation and potentiates the survival of axotomized retinal ganglion cells. , Neuroscience Letters. 2006, 395: 255-260. |
So K.F., Liang Y., You S., Tay D.K.C., Schneider G.E. and Ellis-Behnke R.G., Promotion of axonal growth by CNTF in a permissive environment of self-assembling peptide nanofiber scaffold for brain lesion repair and functional return of vision in adult hamsters, Society for Neuroscience. 2006, Program No. 522.14. |
Researcher
: Tipoe GL |
Project Title: |
Does nitric oxide modulate the development of liver fibrosis? |
Investigator(s): |
Tipoe GL |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2004 |
Completion Date: |
10/2006 |
Abstract: |
To determine the level of oxidative stress in our experimental model; to determine the expression of NO through its enzyme activity such as inducible NO synthase (iNOS) and eNOS mRNAs; to quantify the amount of fibrotic formation; to determine the activity of α-SMA, MMPs TIMPs and TGF-β1; to determine the levels of transcriptional factors such as NF-kappaB and AP-1. |
Project Title: |
Glycolytic adaptive response mediated by hypoxic inducible factors in chronic liver hypoxia |
Investigator(s): |
Tipoe GL, Fung ML |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2005 |
Abstract: |
Prolonged hepatic hypoxia is a common
underlying event in most chronic liver diseases. The metabolic response for
adaptation in chronic liver hypoxia is poorly understood. Chronic hypoxia
results in an increase in nutrient supply which provides cells with energy
for survival. This is accomplished by increase uptake of glucose through the
plasma membrane via glucose transporter 1, 2 and 3. Hypoxia-inducible factors
(HIFs) activate transcription of genes encoding proteins that mediate the
adaptive response to hypoxia. These genes include erythropoietin, VEGF and
glycolytic enzymes. Hypoxia inducible factors are the key transcriptional
factor that regulate vascular motor response and cellular metabolism through
their down-stream genes carrying hypoxic response element (HRE). HIFs play an
essential role in embryonic development, tumor pathogenesis and tissue
ischemia (Huang & Bunn, 2003). In our previous study, we have shown that
chronic hypoxia induces upregulation of HIF-1 alpha and their down-stream HRE
genes involved in the regulation of the vasomotor response such as VEGF, eNOS
and iNOS (Tipoe et al., 2004). These genes produce and maintain the levels of
nitric oxide which counteracts the vasoconstrictive effect of ET-1 (Lau et
al., 2005). Apart from the vasodilation at the sinosiodal level, the HRE
genes mentioned above also support cellular proliferation and survival either
direcctly acting on the liver cells or indirectly by increasing hepatic
perfusion. Up todate, these findings have not been demonstrated by others.
Hence we would like to extend the current work in relation to the metabolic
adaptive mechainism in chronic liver hypoxia by assessing the diffenrent
types of glucose transporter genes that regulate the uptake of glucose in the
liver cells. The transport of glucose in mammalian cells is mediated by a
class of proteins known as glucose transporters. There are 13 isoforms of
glucose transporter and classsified into three clasess (Joost et al., 2002).
Our major interest is on class I [Glucose transporter 1-4] because Glucose
transporter 1 and 3 are induced by hypoxia and Glucose transporter -2 is also
found in liver (Heidbreder et al., 2003). Glucose transporter 1 and 3 are
transcriptionlly regulated by HIF-1 alpha and are considered HRE genes
whereas Glucose transporter -2 is a non-HRE gene. Glucose transporter -1 is
upregulated in glucose deprivation under hypoxic condition in cultured
myocytes and 3T3 fibroblasts and alveolar epithelial cells (Wertheimer et
al., 1991). Alterations in Gluts 1 has been observed in an alcoholic rat
liver from Glucose transporter -2 to Glucose transporter -1 activity in the
centrilobular hepatocytes where hypoxia is more severe (Nanji et al., 1995).
As to date, there are no studies that show the regulation of Glucose
transporter 1, 2 and |
Project Title: |
(-) Epigallocatechinc-3- gallate (EGCG) modulates the progression of liver fibrosis in vivo |
Investigator(s): |
Tipoe GL, Fung ML |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
10/2006 |
Abstract: |
Our in vivo model, the induction of hepatoxin (CCl4) would initiate lipid peroxidation and oxidative stress. In prolonged carbon tetrachloride [CCl4] administration, the presence of oxidative stress would induce chronic inflammation and later would initiate repair and remodelling process in the liver known as fibrosis. Tumor ncerosis factor alpha, iNOS and COX-2 are some of the pro-inflammatory mediators that are commonly induced in chronic liver injury. These pro-inflamatory mediators are mediated by nuclear transcription factors such NF-kB. The extent of liver remodelling would depend on the balance between collagen formatoin and degradation. The key molecules are TGF-beta 1 (mainly produced by the Kupffer cells), metalloproteinases and tissue inhibitor metalliproteinases (mainly produced by hepatic stellate cells). Green tea polyphenols has been known to possess anti-inflammatory, anti-oxidative, anti-fibrongenetic and anti-carcinogenic effects (Hertog et al., 1997; Alvarez et al., 2002; Higdon and Frei, 2003). (-) Epigallocatechins-3-gallate is one of the components of green tea polyphenols which has the strongest anti-oxidant effect. Currently, there are a few studies that investigate the effects of EGCG in liver fibrosis. Most of these studies are in vitro models (Nakamuta et al., 2005; Sakata et al., 2004). Our current study hypothesize that EGCG could modulate the oxidative stress, inflammation and pro-fibrotic mediators by down-regulating the nuclear transcription factors, thereby attenuate the development of liver fibrosis in vivo.The present study has three main objectives: 1) To determine whether EGCG could reduce the level of oxidative stress and the degree of inflammation during the development of liver fibrosis. 2) To investigate whether EGCG could modulate the expression of pro-fibrotic factors such as TGF-beta, procollagen, MMP-2, MMP-9, TIMP-1 and TIMP-2 during the development liver fibrosis.. 3) To evaluate whether EGCG could down-regulate the expression of nuclear transcirption factor, NF-kB, in liver fibrosis. Our group previously showed that EGCG effectively downregulated carbon tetrachloride (CCl4)-induced acute liver injury in association with reduced expression of proinflammatory mediators (iNOS and COX-2) (Chen et al., 2004). Our preliminary findings on the effect of EGCG in CCl4-induced liver fibrosis showed that CCl4-induced liver fibrosis was significantly reduced with EGCG treatment as indicated by the reduced serum ALT level, expression of TNF-alpha and the amount of collagen accumulated in the liver (Sirius Red staining). EGCG also effectively suppressed the formation of nitrotyrosine (as determined by Western Blotting analysis), which is an oxidative stress marker, indicating the antioxidant effect of EGCG. Furthermore, administration of EGCG decreased the expression of iNOS, COX-2 and alpha-smooth muscle actin (alpha-SMA; a marker of activated hepatic stellate cell) . These preliminary results revealed the potent effect of EGCG in mitigating the progression of liver fibrosis by inhibiting inflammatory response and oxidative stress. The future work will adress the last two objectives of this study.References:Alvarez E et al., 2002 Int Immunopharmacol. 2(6):849-855.Chen et al., 2004 Am J Clin Nutr 80: 742-751.bHertog MG et al., 1997 Lancet. 349(9053):699.Higdon JV and Frei B. 2003 Crit Rev Food Sci Nutr. 43(1):89-143.Nakamuta M et al., 2005 Int J Mol Med. 16:677-681.Sakata R et al., 2004 J Hepatol. 40:52-59. |
List of Research Outputs |
Cheung R.T.F., Tipoe G.L., Tam S., Ma E.S.K., Zou L. and Chan P.S., Preclinical evaluation of pharmacokinetics and safety of melatonin in propylene glycol for intravenous administration, Journal of Pineal Research. 2006, 41: 337-343. |
Guo
X., Irwin M.G., Chan D., Tipoe G.L. and Cheung K.M.C., The Role of
Cyclooxygenase 1 and |
Hung M.W., Tipoe G.L., Poon A.M.S., Shiu S.Y.W. and Fung M.L., Anti-inflammatory effect of melatonin on hippocampal injury in rats in intermittent hypoxia, 11th Research Postgraduate Symposium HKU. 2006, 2: 14. |
Hung M.W., Tipoe G.L., Poon A.M.S., Shiu S.Y.W. and Fung M.L., Anti-inflammatory effect of melatonin on the aortic artery in rats exposed to intermittent hypoxia, Journal of the Hong Kong College of Cardiology. 2006, 14(2):88. |
Hung M.W., Tipoe G.L., Poon A.M.S., Shiu S.Y.W. and Fung M.L., The protective effect of melatonin on hippocampal injury of rats in intermittent hypoxia, FASEB Journal . 2007, 21(6): A824: 733.11. |
Hwang I.S.S., Fung M.L., Liong E.C., Tipoe G.L. and Tang F., Age-related changes in adrenomedullin expression and hypoxia-inducible factor 1 activity in the rat lung and their responses to hypoxia, The Fourth International Huaxia Congress of Endocrinology. 2007. |
Hwang
I.S.S., Fung M.L., Liong E.C., Tipoe G.L. and Tang F., Age-related changes in
adrenomedullin expression and hypoxia-inducible factor-1 activity in the rat
lung and their responses to hypoxia., Journal of Gerontology. 2007, |
Hwang
I.S.S., Fung M.L., Liong E.C., Tipoe G.L. and Tang F., Age-related changes in
adrenomedullin expresssion and hypoxia-inducible factor-1 activity in the rat
lung and their responses to hypoxia, Journal of Gerontology:Biological
Sciences. 2007, |
Lam S.S.Y., Tipoe G.L., Liong E.C. and Fung M.L., Expressions of hypoxia-inducible factor-1alpha, -2alpha, -3alpha in the rat carotid body during chronic and intermittent hypoxia, Journal of the Hong Kong College of Cardiology. 2006, 14(2):89. |
Lam S.S.Y., Tipoe G.L., Tjong Y.W., Liong E.C. and Fung M.L., Intermittent hypoxia regulates the renin-angiotensin system in the rat carotid body, 11th Research Postgraduate Symposium HKU. 2006, 2:16. |
Lam
S.S.Y., Tipoe G.L., Liong E.C., Leung P.S. and Fung M.L., Up-regulation of a local
renin-angiotensin system in the rat carotid body during chronic intermittent
hypoxia, The Physiology Symposium (Hong Kong-Taiwan) |
Leung T.M., Liong E.C., Fung M.L., Lau T.Y.H., Nanji A.A. and Tipoe G.L., Inhibition of inducible nitric oxide synthase (iNOS) or administration of L-arginine reverses the induction of hypoxia-inducible factor (HIF-1a) in experimental liver fibrosis, AALSD Meeting, San Francisco USA. 2006. |
Pan N.Y., Hui W.S., Tipoe G.L., Taylor G.W., Leung Y.H., Lam W.K., Tsang K.W.T. and Mak J.C.W., Inhibition of pyocyanin-potentiated IL-8 release by steroids in bronchial epithelial cells, Respiratory medicine. 2006, 100(9): 1614-1622. |
Tipoe
G.L., Ho
M.C.T., Liong E.C., Leung T.M., Lau T., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcholic fatty liver disease (NAFLD) not
requiring a high fat diet, Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho
M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., A clinically
relevant animal model of non-alcoholic fatty liver disease (NAFLD) not
requiring a high fat diet 1275 Suppl, Hepatology. 2006, 44(4): |
Tipoe
G.L., Leung
T.M., Liong E.C., Fung M.L., Lau T. and Nanji A.A.,
Antioxidant and antiinflammatory effects of green tea polyphenols in carbon
tetrachloride induced liver fibrosis in mice, Hepatology. 2006, 44(4)
Suppl. 1: |
Tipoe
G.L., Ho
M.C.T., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorate pathological changes, oxidative stress and
pro-inflammatory markrs in an animal model of non-alcoholic fatty liver
disease [NAFLD], Hepatology. 2006, 44(4) Suppl. 1: |
Tipoe
G.L., Ho
M.C.T., Liong E.C., Leung T.M., Lau T.H.Y., Fung M.L. and Nanji A.A., Green tea
polyphenols ameliorated pathological changes, oxidative stress and
pro-inflammatory markers in an animal model of non-alcoholic fatty liver
disease (NAFLD) animal model 1063 Suppl 1, Hepatology . 2006, 44(4): |
Tipoe G.L., Leung T.M., Hung M.W. and Fung M.L., Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection, Cardiovascular & Hamatological Disorders Drug Targets. 2007, 7(2): 135-144. |
Tipoe G.L., Leung T.M., Hung M.W. and Fung M.L., Green tea polyphenols as an anti-oxidant and anti-inflammatory agent for cardiovascular protection, Cardiovascular & Hematological Disorders - Drug Targets. 2007, 7(2): 135-144. |
Tipoe G.L., Leung T.M., Liong E.C., So H.S.H., Leung K.M., Lau T.Y., Tom W.M., Fung M.L., Fan S.T. and Nanji A.A., Inhibitors of inducible nitric oxide (NO) synthase are more effective than an NO donor in reducing carbon-tetrachloride induced acute liver injury, Histology and Histopathology. 2006, 21(11): 1157-1165. |
Tipoe
G.L., Leung
T.M., Liong E.C., Fung M.L., Lau T.Y.H. and Nanji A.A.,
Lau and A.A. Nanji. Antioxidant and antiinflammatory effects of green tea
polyphenols in carbon tetrachloride induced liver fibrosis in mice, Hepatology.
2006, 44(4): |
Researcher
: Tsao GSW |
Project Title: |
Defining the genetic elements involved in immortalization and malignant transformation of primary nasopharyngeal epithelial cells |
Investigator(s): |
Tsao GSW, Jin Y |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
10/2004 |
Abstract: |
To examine the roles of inactivation of the P16 and RASSFIA genes, overexpression of deltaNp63 and telomerase reconstitution in the immortatlization of primary nasopharyngeal epithelial cells; to establish an immortalized nasopharyngeal epithelial cells using defined genetic elements for NPC oncogenesis study; to examine the susceptibility of immortalized nasopharyngeal cells to the malignant transformation by EBV latent genes/EBV infection. |
Project Title: |
Mapping and identification of gene(s) on chromosome 20q involved in cell immortalization |
Investigator(s): |
Tsao GSW, Cheung A, Kwong YL |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2004 |
Completion Date: |
10/2006 |
Abstract: |
To map and define the regions of amplification on chromosome 20q in immortalized cells; to identify the gene on chromosome 20q overexpressed in immortalized cells; to carry our functional analysis of the overexpressed genes on chromosome 20q in normal epithelial cells before immortalization. |
Project Title: |
Functional study of EBV infection and genetic alterations in immortalized epithelial cells |
Investigator(s): |
Tsao GSW, Chen H |
Department: |
Anatomy |
Source(s) of Funding: |
NSFC/RGC Joint Research Scheme |
Start Date: |
12/2004 |
Abstract: |
To study intracellular signaling events
regulating latent infection of EBV infection in nasopharyngeal epithelial
cells; to study the role of the EBV encoded LMP |
Project Title: |
Chromosome instabiliy induced by Id1 expression in nasopharyngeal epithelial cells |
Investigator(s): |
Tsao GSW |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
12/2005 |
Abstract: |
BackgroundChromosome instability is a
hallmark of human cancers. Immortalization is a pre-requisite property of all
cancer cells and is considered an early event in the carcinogenesis process.
Events occurring at the immortalization process may be earlier steps involved
in carcinogenesis. Nasopharyngeal carcinoma is a common cancer among Southern
Chinese. Our laboratory has been involved in investigation of critical events
underlying the immortalization and malignant transformation of human
nasopharyngeal epithelial cells. Several events are critical for
immortalization of nasopharyngeal epithelial cells. Among them are telomerase
activation, p16 inactivation and downregulation of p21 gene which are
commonly observed in our immortalized nasopharyngeal epithelial cells. In
addition, we observed that Id1 expression is common in immortalized
nasopharyngeal epithelial cells. Id1 expression could suppress p16 and p21
expression in cells and may play a role in cell immortalization. We have
previously reported that Id1 is commonly overexpressed in nasopharyngeal
carcinoma (Wang et al., 2002). Id1 expression could accelerate cell cycle
progression in nasopharyngeal carcinoma cell. Interestingly, we observed that
an Epstein Barr virus encoded protein- LMP1 could effectively upregulate Id1
expression in an immortalized nasopharyngeal epithelial cell established in
our laboratory (Tsao et al., 2002; Li HM et al., 2004 ). In order to study
the functional significance of Id1 expression in nasopharyngeal epithelial
cells, we have overexpressed Id |
Project Title: |
Mechanisms involved in Id1 induced centrosome abnormalities |
Investigator(s): |
Tsao GSW, Wong YC |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2006 |
Abstract: |
(1) To examine and confirm the involvement of Aurora A expression and NFkB in Id1 induced centrosome abnormalities in cells. (2) To elucidate the events involved in the actigvation of NFkB by Id1. |
Project Title: |
Downregulation
of RASSF |
Investigator(s): |
Tsao GSW, Deng W, Cheung A |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
01/2007 |
Abstract: |
Background and Objectives: Nasopharyngeal
carcinoma (NPC) is a common disease among Cantonese population living in Hong
Kong and southern |
List of Research Outputs |
Chan
D.W., Liu V.W.S., Furukawa T., Tsao G.S.W., Yao K.M., Chan K.K.L. and Ngan H.Y.S., Loss of MKP3 is associated
with cisplatin-resistance and tumorigenicity of ovarian cancer, American
Association for Cancer Research. Annual Meeting 2007. |
Cheung A., Cheung P.Y., Deng W. and Tsao G.S.W., Immortalization of human esophageal epithelial cells by human telomerase reverse transcriptase (hTERT), Proceedings o the American Association for Cancer Research Annual Meeting, April 14-18, Los Angeles, U. S. A.. 2007, No. 4268. |
Chu Q., Lee D.T.W., Tsao G.S.W., Wang X. and Wong Y.C., S-allycysteine, a water-soluble garlic derivative, suppresses the growth of a human androgen-independent prostate cancer xenograft, CWR22R, under in vivo conditions, BJU International. 2006, 99: 925-932. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-Beta1 sensitivity in prostate epithelial cells, Biology of the Cell. 2006, 98(9): 523-533. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-β1 sensitivity in prostate epithelial cells. , Biology of the Cell. 2006, 98: 523-533. |
Jin Y., Tsao G.S.W. and Kwong Y.L., Re: Wilting et al. Increased gene copy numbers at chromosome 20q are frequent in both squamous cell carcinomas and adenocarcinomas of the cervix, The Journal of Pathology. 2006, 209: 220-230. |
Lau
E.P.W., Tsao G.S.W., Tong Y. and Feng Y., Study on the anti proliferative
effect and its mechanism of berberine in various cancer cell lines. , 2006
Hong Kong-Macau Postgraduate Symposium on Chinese Medicine. . |
Lau
W.M., Tsao G.S.W., So K.F. and Yip H.K.F., Expression Of Telomerase
Reverse Transcriptase In Adult Goldfish Retina, J. Molecular Neuroscience.
|
Li B., Cheung P.Y., Wang X., Tsao G.S.W., Ling M.T., Wong Y.C. and Cheung A., Id-1 protects esophageal cancer cells from TNF-a-induced apoptosis through activation of P13K/AKT/NFkb signaling pathway, Proceedings of the Aerican Association for Cancer Research Annual Meeting, Los Angeles, U. S. A., April 14-18, 2007.. 2007, No. 5162. |
Lung H.L., Cheung A.K.L., Xie D., Cheng Y., Kwong F.M., Murakami Y., Guan X.Y., Sham J.S.T., Chua D.T.T., Protopopov A.I., Zabarovsky E.R., Tsao G.S.W., Stanbridge E.J. and Lung M.L., TSLC1 is a tumor suppressor gene associated with metastasis in nasopharyngeal carcinoma, Cancer Research. 2006, 66(19): 9385-9392. |
Man
C.W.Y., Doxsey S., Rosa J. and Tsao
G.S.W., Id1 induces centrosome abnormalities, disrupt microtubule
integrity and stabilizes Aurora A in human epithelial cells. , In: CSHL, |
Mönkkönen K.S., Aflatoonian R., Lee C.K.F., Yeung W.S.B., Tsao G.S.W., Laitinen J.T.,
Tuckerman E.M., Li T.C. and Fazeli A., Localization and variable expression
of Galphai |
Nicholls J.M., Chan M.C.W., Chan W.Y., Wong C.H.K., Cheung C.Y., Kwong D.L., Wong M.P., Chui W.H., Poon L.L.M., Tsao G.S.W., Guan Y. and Peiris J.S.M., Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract, In: Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract, Nature Medicine. 2007, 13(2): 147-9. |
Nicholls J.M., Chan M.C.W., Chan W.Y., Wong H.K., Kwong D.L.W., Wong M.P., Chui W.H., Poon L.L.M., Tsao G.S.W., Guan Y. and Peiris J.S.M., Tropism of avian influenza A (H5N1) in the upper and lower respiratory tract, Nature Medicine. 2007, 13(2): 147-149. |
Tang W.K., Chui C.H., Fatima S., Kok S.H., Pak K.C., Ou T.M., Hui K.S., Wong M.M., Wong J., Law S.Y.K., Tsao G.S.W., Lam K.Y., Beh P.S., Srivastava G., Ho K.P., Chan A.S. and Tang J.C.O., Inhibitory effects of gleditsia sinensis fruit extract on telomerase activity and oncogenic expression in human esophageal squamous cell carcinoma, International Journal of Molecular Medicine. 2007, 19(6): 953-960. |
Tang W.K., Chui C.H., Fatima S., Kok S.H., Pak K.C., Ou T.M., Hui K.S., Wong M.M., Wong J., Law S.Y.K., Tsao G.S.W., Lam K.Y., Beh P.S., Srivastava G., Chan A.S., Ho K.P. and Tang J.C.O., Oncogenic properties of a novel gene JK-1 located in chromosome 5p and its overexpression in human esophageal squamous cell carcinoma, International Journal of Molecular Medicine. 2007, 19(6): 915-923. |
Tsai C.L., Li H.P., Lu Y.J., Hsueh C., Liang Y., Chen C.L., Tsao G.S.W., Tse K.P., Yu J.S. and Chang Y.S., Activation of DNA methyltransferase 1 by EBV LMP-1 involves c-Jun NH2-terminal kinase signaling, Cancer Research. 2006, 66: 11668-11676. |
Tsao G.S.W., Current Cancer Drug Targets. 2006. |
Tsao
G.S.W., Induction of mitotic dysregulation
and chromosomal aberrations by the Epstein Barr-virus encoded LMP1 protein:
Implication in the pathogenesis of nasopharyngeal carcinoma. , 7th
International Chromosome Segregatin and Aneuploidy Workshop. June 16-20, |
Wang L.D., Qin Y., Fan Z., Kwong D.L.W., Guan X.Y., Tsao G.S.W., Sham J., Li J.L. and Feng X.S., Comparative genomic hybridization: comparison between esophageal squamous cell carcinoma and gastric cardia adenocarcinoma from a high-incidence area for both cancers in Henan, northern China., Diseases of Esophagus. 2006, 19: 459-467. |
Yau W.L., Lung H.L., Zabarovsky E.R.,
Lerman M.I., Sham J.S.T., Chua D.T.T., Tsao G.S.W., Stanbridge E.J. and
Lung M.L., Functional studies of the chromosome 3p21.3 candidate tumor
suppressor gene BLU/ZMYND |
Zhang H., Jin Y., Chen X., Jin C., Law S.Y.K., Tsao G.S.W. and Kwong Y.L., Papillomavirus type 16 E6/E7 and human telomerase reverse transcriptase in esophageal cell immortalization and early transformation, Cancer Letters. 2007, 245(1-2): 184-194. |
Researcher
: Wang L |
List of Research Outputs |
Guo J., Zeng Y.S., Liang Y., Wang L., Su H. and Wu W., Cyclosporine affects the proliferation and differentiation of neural stem cells in culture, NeuroReport. 2007, 18(9): 863-868. |
Researcher
: Wang X |
Project Title: |
Downregulation of MAD2 expression and its significance in chemodrug sensitization in nasopharyngeal carcinoma cells |
Investigator(s): |
Wang X, Nicholls JM, Tsao GSW, Jin D |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2003 |
Abstract: |
To confirm that MAD2 expression is downregulated in clinical samples of NPC; to determine the mechanism by which MAD2 is downregulated in NPC; to establish whether upregulation of MAD2 expression can sensitize NPC cells to chemotherapy. |
Project Title: |
The role fo MAD |
Investigator(s): |
Wang X, Jin D |
Department: |
Anatomy |
Source(s) of Funding: |
Lance Armstrong Foundation - General Award |
Start Date: |
10/2003 |
Abstract: |
To investigate the association between
decreased MAD2 protein expression and cisplatin resistance in TGCT cells; to
study if exogenous expression of the MAD2 gene in TGCT cells can lead to
chemosensitization to cisplatin in TGCT cells; to demonstrate that
downregulation of MAD2 results in resistance ot cisplatin in TGCT cells; to
characterize the role of MAD |
Project Title: |
Significance of MAD2 expression to chromosomal instability in prostate cancer |
Investigator(s): |
Wang X, Jin D Y |
Department: |
Anatomy |
Source(s) of Funding: |
Association for International Cancer Research - General Award |
Start Date: |
04/2004 |
Abstract: |
To correlate MAD2 expression with genomic instability in prostate cancer specimens; to show that MAD2 expression is essential for a functional mitotic checkpoint in prostate cancer cells; to demonstrate that downregulation of MAD2 leads to mitotic checkpoint defect and increased CIN in prostate cancer cells. • To investigate if promoter hypermethylation contributes to decreased MAD2 expression in prostate cancer |
Project Title: |
Molecular mechanisms involved in the suppressive effects of garlic derivatives on cell growth and motility in prostate cancer cells |
Investigator(s): |
Wang X, Jin D Y |
Department: |
Anatomy |
Source(s) of Funding: |
American Institute for Cancer Research (AICR) - General Award |
Start Date: |
08/2005 |
Abstract: |
To study the role of the mitotic checkpoint in SAC and SAMC-induced androgen independent prostate cancer cell death; to investigate if SAC and SAMC have any inhibitory effect on the invasive ability of prostate cancer cells and to determine the molecular mechanisms responsible. Specific Aim 3. To examine if SAC and SAMC can enhance the sensitivity of prostate cancer cells to chemotherapeutic drugs. |
Project Title: |
Significance of Id-1 upregulation and its association with EGFR in bladder cancer |
Investigator(s): |
Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
06/2006 |
Abstract: |
Background: Bladder cancer is one of the
common urological cancers. Although majority of bladder cancers are
superficial at presentation, 20% of the bladder cancer patients present with
muscle invasive disease at diagnosis (i.e. stage T2-T4 tumours). If
untreated, fewer than 15% of those patients can survive more than two years.
Two main challenges remain for the treatment of this cancer which are (i) the
ability to identify the small but significant number of patients with
non-muscle-invasive disease who will progress to muscle-invasive disease, and
(ii) to improve current treatment of the muscle invasive bladder cancer.
Recently, our group identified a potential oncogene, Id-1 (inhibitor of
differentiation or DNA binding), and demonstrated its significance in the
development of human prostate cancer (Ouyang et al., J Urol 167: 2598, 2002),
nasopharyngeal carcinoma (Wang et al., 35:42-49, 2002) and ovarian cancer
(Zhang et al., Br J Cancer 91: 2042, 2004). Since then, upregulation of Id-1
has been reported in over 20 types of human cancer such as breast, pancreas,
cervical, melanoma, and head and neck (Reviewed by Wong et al., 9:279-289,
2004). In addition, increased Id-1 expression levels are associated with
advanced tumour stage as well as poor prognosis (Maruyama et al., Am J Pathol
155: 815, 1999; Schindl et al., Cancer Res 61: 5703, 2001; Ouyang et al., J
Urol 167: 2598, 2002). Furthermore, patients with higher levels of Id-1 have
much shorter overall survival than the patients with relatively lower Id-1
expression in ovarian cancer (Schindl et al., Clin Cancer Res 9:779, 2003).
Recent evidence also shows that Id-1 is able to induce epidermal growth
factor receptor (EGFR) expression, indicating that the oncogenic effect of
Id-1 may be mediated through activation of the EGFR pathway (Ling et al.,
Carcinogenesis 25: 517, 2004; Zhang et al., Br J Cancer 91: 2042, 2004). In
bladder cancer, upregulation of EGFR has been frequently reported in tissue
samples (Neal et al., Lancet 1: 366, 1985; Mellon et al., J Urol 153: 919,
1995). Several studies have correlated EGFR positively with tumour stage,
tumour progression, and poor clinical outcome in bladder cancer patients
(Neal et al., Cancer 65: 1619, 1990; Mellon et al., J Urol 153: 919, 1995;
Nguyen et al., Am J Clin Pathol 101: 166, 1994). Furthermore, it was recently
reported that treatment of bladder cancer cells with ZD1839 (or Iressa), a
highly selective EGFR inhibitor, resulted in radiosensitization to ionizing
radiation (Maddineni et al., Br J Cancer 92: 125, 2005), indicating that EGFR
may be a potential therapeutic target in improving treatment efficiency of
bladder cancer. Based on the evidence that Id-1 is able to activate EGFR
pathway, we hypothesized that activation of the EGFR pathway observed in
bladder cancer may be a result of overexpression of Id-1, as indicated in
other cancers (Ling et al., Carcinogenesis 25: 517, 2004; Zhang et al., Br J
Cancer 91: 2042, 2004 ). Purpose of the project: The aim of this project is
to study the significance of Id |
List of Research Outputs |
Chu
Q., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Garlic derivatives inhibit
prostate cancer cell growth and invasion through restoration of E-cadherin
expression, 8th Asian Congress of Urology, |
Chu Q., Lee D.T.W., Tsao G.S.W., Wang X. and Wong Y.C., S-allycysteine, a water-soluble garlic derivative, suppresses the growth of a human androgen-independent prostate cancer xenograft, CWR22R, under in vivo conditions, BJU International. 2006, 99: 925-932. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-Beta1 sensitivity in prostate epithelial cells, Biology of the Cell. 2006, 98(9): 523-533. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-β1 sensitivity in prostate epithelial cells. , Biology of the Cell. 2006, 98: 523-533. |
Fung K.L., Cheung H.W., Wong H.L., Yuen H.F., Ling M.T., Chan K.W., Wong Y.C., Cheung A. and Wang X., MAD2 expression and its significance in mitotic checkpoint control in testicular germ cell tumour. , Biochimica et Biophysica Acta - Molecular Cell Research . 2007, 1773: 821-832. |
Fung K.L., Cheung H.W., Ling M.T., Cheung A., Wong Y.C. and Wang X., Role of MEK/ERK pathway in the MAD2-mediated cisplatin sensitivity in testicular germ cell tumour cells, British Journal of Cancer. 2006, 95: 475-484. |
Howard E.W., Chua C.W., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Potent suppression of distant metastasis by garlic compound S-allylmercaptocysteine in an in vivo androgen independent prostate cancer model, AACR Special Conference in Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006, San Francisco, U. S. A.. 2006, A1. |
Lee K.W., Poon R.T.P., Yuen P.W., Ling M.T., Wang X., Wong Y.C., Guan X.Y., Man K., Tang Z.Y. and Fan S.T., Regulation of angiogenesis by Id-1 through hypoxia-inducible factor-1 mediated vascular endothelial growth factor up-regulation in hepatocellular carcinoma, Clinical Cancer Research. 2006, 12(23): 6910-6919. |
Lee K.W., Poon R.T.P., Yuen P.W., Ling M.T., Kwok W.K., Wang X., Wong Y.C., Guan X.Y., Man K., Chau K.L. and Fan S.T., Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition, Clinical Cancer Research. 2006, 12(18): 5369-5376. |
Li B., Cheung P.Y., Wang X., Tsao G.S.W., Ling M.T., Wong Y.C. and Cheung A., Id-1 protects esophageal cancer cells from TNF-a-induced apoptosis through activation of P13K/AKT/NFkb signaling pathway, Proceedings of the Aerican Association for Cancer Research Annual Meeting, Los Angeles, U. S. A., April 14-18, 2007.. 2007, No. 5162. |
Wong Y.C., Zhang X., Ling M.T. and Wang X., Inactivation of Id-1 gene induces sensitivity of prostate cancer cells to chemotherapeutic drugs, 5th International Symposium on Hormonal Carcinogenesis, Mountpellier, France, September 10-13, 2006. |
Wong
Y.C., Chu Q., Lee D.T.W. and Wang X., S-allylcysteine (SAC), a
garlic derivative, suppresses the growth fo androgen-independent prostate
cancer xenograft under in vivo conditiion, AACR Special Conference in
Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006,
|
Xu K., Ling M.T., Wang X. and Wong Y.C., Evidence of a novel biomarker, s1-Casein, a milk protein, in benign prostatic hyperplasia., Prostate cancer and Prostate Diseases. 2006, 9: 293-297. |
Yuen
H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Id proteins expression in
prostate cancer: high-level expression of Id |
Yuen H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Significance of TWIST and E-cadherin expression in the metastatic progression of prostatic cancer, Histopathology. 2007, 50(5): 648-58. |
Yuen H.F., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., The prognostic significance of Id proteins in Esophageal Squamous Cell Carcinoma, The 97th American Association of Cancer Research Annual Meeting, Los Angeles, USA, April. 2007. |
Yuen H.F., Chan Y.P., Wong M.L.Y., Kwok W.K., Chan K.K., Lee P.Y., Srivastava G., Law S.Y.K., Wong Y.C., Wang X. and Chan K.W., Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis, Journal of Clinical Pathology. 2006, 60(5): 510-514. |
Zhang Z., Xie D., Li X., Wong Y.C., Xin D., Guan X.Y., Chua C.W., Leung S.C., Na Y. and Wang X., Significance of TWIST expression and its association with E-cadherin in bladder cancer., Human Pathology. 2007, 38: 598-606. |
Researcher
: Wong HL |
List of Research Outputs |
Fung K.L., Cheung H.W., Wong H.L., Yuen H.F., Ling M.T., Chan K.W., Wong Y.C., Cheung A. and Wang X., MAD2 expression and its significance in mitotic checkpoint control in testicular germ cell tumour. , Biochimica et Biophysica Acta - Molecular Cell Research . 2007, 1773: 821-832. |
Researcher
: Wong YC |
Project Title: |
Induction of breast carcinogenesis by a combination of androgens and oestrogens |
Investigator(s): |
Wong YC, Xie B |
Department: |
Anatomy |
Source(s) of Funding: |
Other Funding Scheme |
Start Date: |
07/1997 |
Abstract: |
To examine the role of both oestrogens and androgens in the initiation and progression of breast carcinogenesis in an animal model. |
Project Title: |
The |
Investigator(s): |
Wong YC, Wang X, Ho KMT |
Department: |
Anatomy |
Source(s) of Funding: |
The |
Start Date: |
07/2003 |
Abstract: |
To investigate molecular mechanisms responsible for racial differences in prostate cancer incidence. |
Project Title: |
Id-1 activation
of |
Investigator(s): |
Wong YC, Tsao GSW, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2003 |
Abstract: |
To investigate the correlation between the over-expression of Id-1 and activation of NF-[kappa]B in prostate cancer progression; to examine the sensitivity of LNCaP-Id-1 clones towards TNF-[alpha] induced apoptosis; to explore the effect of inhibition of Id-1 expression by antisense Id-1 oligonucleotide on TNF[alpha] induced apoptosis; to study the role of Id-1 gene in development of AI prostate cancer in vivo; to investigate the effect of Id-1 gene silencing on growth of prostate cancer using RNA interference on human prostate cancer xenograft, CWR22. |
Project Title: |
Identification and evaluation of specific marker proteins from secretions of benign prostatic hyperplasia (BPH) |
Investigator(s): |
Wong YC, Tam PC, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
NSFC/RGC Joint Research Scheme |
Start Date: |
12/2003 |
Abstract: |
To confirm and characterize these proteins in the secretion of BPH by 2-D gel electrophoresis followed by mass spectrometry and mass sequencing; to examine the sources of these differetially expressed proteins including the PSP61, lwPSA and protein X, and to investigate the value of these proteins as markers of BPH; to examine whether these proteins are present in serum and to examine the potential of using these proteins as markers in clinical diagnosis of PBPH; to assess correlation of the levels of these newly identified proteins to PSA levels in prostate cancer and BPH patient and to examine their association with PSA. |
Project Title: |
The role of Id-1 gene in initiation of prostate carcinogenesis |
Investigator(s): |
Wong YC, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
01/2005 |
Abstract: |
To investigate whether over-expression of Id1 could lead to extension of the life span, possibly immortalization, of HPrE cells; to examine whether Id1 expression in combination with additional factors is able to induce malignant transformation of HPrE cells; to study the molecular mechanisms responsible for the Id1-induced malignant transformation of HPrE cells; to identify novel factors (pathways) responsbile for Id1-induced malignant transformation of HPrE cells. |
Project Title: |
The role of ld-1 gene in prostate cancer angiogenesis |
Investigator(s): |
Wong YC |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2006 |
Abstract: |
Id-1 (inhibitor of differentiation/DNA
synthesis) protein belongs to the Id family of helix-loop-helix proteins. It
lacks the basic domain for DNA binding and functions mainly as a dominant
inhibitor of the bHLH transcription factor through heterodimerization (1).
Id-1 has been shown to play a critical role in the regulation of cell
proliferation (2), differentiation (3) and senescence (4-6), and recent
studies suggest that Id-1 may function as an oncogene. For example, Id-1 is
shown to inhibit replicative senescence and promotes life span of primary
cells through inactivation of p16/RB pathway (6). In addition, elevated Id-1
expression either at transcriptional or translational levels has been
reported in over 20 types of human cancer including prostate, breast,
cervical, colon, liver cancers (7). Furthermore, ectopic expression of Id-1
is able to promote cancer cell proliferation and protect against apoptosis
under sub-optimal culture conditions (2, 8). In addition to its potential
oncogenic actions, Id-1 has also been suggested to take part in the malignant
progression of human cancer. For example, in breast cancer, Id-1 is found to
be constitutively expressed in the highly aggressive but not the
non-aggressive cancer cells (9). In endometrial carcinoma, Id-1 expression is
high in high grade and invasive tumors (10). Early stage cervical cancer
patients with high Id-1 expression have poor prognosis compared with patients
with relatively low Id-1 expression (11). In breast and cervical cancers,
increased Id-1 is associated with more aggressive clinical behavior as well
as poor clinical outcome in patients (9, 11, 12). In our previous studies, in
human prostate cancer, Id-1 expression is found to be increased with
increased Gleason score of the tumors (12) and ectopic expression of Id |
List of Research Outputs |
Chu
Q., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Garlic derivatives inhibit
prostate cancer cell growth and invasion through restoration of E-cadherin
expression, 8th Asian Congress of Urology, |
Chu Q., Lee D.T.W., Tsao G.S.W., Wang X. and Wong Y.C., S-allycysteine, a water-soluble garlic derivative, suppresses the growth of a human androgen-independent prostate cancer xenograft, CWR22R, under in vivo conditions, BJU International. 2006, 99: 925-932. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-Beta1 sensitivity in prostate epithelial cells, Biology of the Cell. 2006, 98(9): 523-533. |
Di K., Ling M.T., Tsao G.S.W., Wong Y.C. and Wang X., Id-1 modulates senescence and TGF-β1 sensitivity in prostate epithelial cells. , Biology of the Cell. 2006, 98: 523-533. |
Fung K.L., Cheung H.W., Wong H.L., Yuen H.F., Ling M.T., Chan K.W., Wong Y.C., Cheung A. and Wang X., MAD2 expression and its significance in mitotic checkpoint control in testicular germ cell tumour. , Biochimica et Biophysica Acta - Molecular Cell Research . 2007, 1773: 821-832. |
Fung K.L., Cheung H.W., Ling M.T., Cheung A., Wong Y.C. and Wang X., Role of MEK/ERK pathway in the MAD2-mediated cisplatin sensitivity in testicular germ cell tumour cells, British Journal of Cancer. 2006, 95: 475-484. |
Howard E.W., Chua C.W., Ling M.T., Cheung H.W., Wang X. and Wong Y.C., Potent suppression of distant metastasis by garlic compound S-allylmercaptocysteine in an in vivo androgen independent prostate cancer model, AACR Special Conference in Cancer Research: Innovations in Prostate Cancer Research, December 6-9, 2006, San Francisco, U. S. A.. 2006, A1. |
Lee K.W., Poon R.T.P., Yuen P.W., Ling M.T., Wang X., Wong Y.C., Guan X.Y., Man K., Tang Z.Y. and Fan S.T., Regulation of angiogenesis by Id-1 through hypoxia-inducible factor-1 mediated vascular endothelial growth factor up-regulation in hepatocellular carcinoma, Clinical Cancer Research. 2006, 12(23): 6910-6919. |
Lee K.W., Poon R.T.P., Yuen P.W., Ling M.T., Kwok W.K., Wang X., Wong Y.C., Guan X.Y., Man K., Chau K.L. and Fan S.T., Twist overexpression correlates with hepatocellular carcinoma metastasis through induction of epithelial-mesenchymal transition, Clinical Cancer Research. 2006, 12(18): 5369-5376. |
Li B., Cheung P.Y., Wang X., Tsao G.S.W., Ling M.T., Wong Y.C. and Cheung A., Id-1 protects esophageal cancer cells from TNF-a-induced apoptosis through activation of P13K/AKT/NFkb signaling pathway, Proceedings of the Aerican Association for Cancer Research Annual Meeting, Los Angeles, U. S. A., April 14-18, 2007.. 2007, No. 5162. |
Wong Y.C., Id-1 gene and development of chemodrug resistance in prostate cancer cells, Chinese Journal of Anatomy. 2006, 29 (Suppl): 153.49. |
Wong
Y.C., Zhang
X., Ling M.T. and Wang X., Inactivation of Id-1 gene
induces sensitivity of prostate cancer cells to chemotherapeutic drugs, 5th
International Symposium on Hormonal Carcinogenesis, |
Wong
Y.C., Research in morphological sciences in |
Wong
Y.C., Chu
Q., Lee D.T.W. and Wang X., S-allylcysteine (SAC), a garlic
derivative, suppresses the growth fo androgen-independent prostate cancer
xenograft under in vivo conditiion, AACR Special Conference in Cancer
Research: Innovations in Prostate Cancer Research, December 6-9, 2006, |
Xu K., Ling M.T., Wang X. and Wong Y.C., Evidence of a novel biomarker, s1-Casein, a milk protein, in benign prostatic hyperplasia., Prostate cancer and Prostate Diseases. 2006, 9: 293-297. |
Yuen
H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Id proteins expression in
prostate cancer: high-level expression of Id |
Yuen H.F., Chua C.W., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., Significance of TWIST and E-cadherin expression in the metastatic progression of prostatic cancer, Histopathology. 2007, 50(5): 648-58. |
Yuen H.F., Chan Y.P., Wong Y.C., Wang X. and Chan K.W., The prognostic significance of Id proteins in Esophageal Squamous Cell Carcinoma, The 97th American Association of Cancer Research Annual Meeting, Los Angeles, USA, April. 2007. |
Yuen H.F., Chan Y.P., Wong M.L.Y., Kwok W.K., Chan K.K., Lee P.Y., Srivastava G., Law S.Y.K., Wong Y.C., Wang X. and Chan K.W., Upregulation of Twist in oesophageal squamous cell carcinoma is associated with neoplastic transformation and distant metastasis, Journal of Clinical Pathology. 2006, 60(5): 510-514. |
Zhang Z., Xie D., Li X., Wong Y.C., Xin D., Guan X.Y., Chua C.W., Leung S.C., Na Y. and Wang X., Significance of TWIST expression and its association with E-cadherin in bladder cancer., Human Pathology. 2007, 38: 598-606. |
Researcher
: Wong YY |
List of Research Outputs |
Yu M.S., Wong Y.Y., So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., New polysaccharide from Nerium indicum protects neurons via stress kinase signaling pathway, Brain Research. 2007, 1153: 221-230. |
Researcher
: Wu W |
Project Title: |
Effects of chondroitinase ABC and lithium chloride on neuronal survival and regeneration after spinal cord injury |
Investigator(s): |
Wu W, Yick LW, So KF |
Department: |
Anatomy |
Source(s) of Funding: |
Matching Fund for National Key Basic Research Development Scheme (973 Projects) |
Start Date: |
11/2003 |
Abstract: |
To study the effects of chondroitinase ABC and lithium chloride on neuronal survival and regneration after spinal cord injury. |
Project Title: |
The role of neuronal nitric oxide synthase in motoneuron degeneration |
Investigator(s): |
Wu W, Huang J |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2004 |
Abstract: |
To examine the effect of nNOS inhibitors on the expression of injury-induced nNOS and the effect on motoneuron survival and regeneration in adult animals following spinal root avulsion; to investigate the effect of blockage of nNOS expression by antisense nNOS oligodeoxynuleotide (ODN) on motoneuron degeneration and regeneration after root avulsion; to examine how motoneurons respond to axonal injury and whether they can regenerate after root avulsion in nNOS knockout animals; to investigate the effect of nNOS gene silencing on survival and growth of cultured spinal motoneurons using RNA interference technique. |
Project Title: |
Effect of LINGO-1 antibody on demyelinating desease |
Investigator(s): |
Wu W |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
01/2006 |
Abstract: |
Backgroud: Demyelinating diseases are
characterized by the loss of myelin sheath due to extensive inflammation and
gliosis in the central nervous system (CNS). A typical example of
demyelinating diseases is multiple sclerosis (MS). The main pathological
characteristics of MS are widespread demyelination and oligodendrocyte
degeneration [1]. Degeneration of oligodendrocytes not only results in
demyelination but also cause degeneration of axons and neurons in the late
stage of MS [2]. Axonal loss and neuronal degeneration in MS contribute
directly to the disability motor and sensory functions [3]. The key issue in
MS is the degenrartion of oligodendrocytes. Therefore, preventing
degeneration of oligodendrocytes may be critical for the progress of MS and
could become a potential theraputical procedure. The control of myelination
by oligodendrocytes in the CNS is poorly understood. Recently, a study in
Mi's group [4,5] demonstrated that LINGO-1 (LRR and Ig domain-containing,
Nogo Receptor-interacting proten) is an important negative regulator of
myelination. LINGO-1 is expressed in oligodendrocytes. Overexpression of LINGO-1
leads to inhibition of oligodendrocyte differentiation and myelination.
Attenuation of its function by dominat-negative LINGO-1, LINGO-1 RNA-mediated
interference (RNAi) or soluble human LINGO-1 (LINGO-1-Fc) leads to
differentiation of oligodendrocytes and myelination competence [5]. The
ability to recapitulate CNS myelination is verified in vivo through the
analsis of LINGO-1 knochout mice [5], which indicate that LINGO-1 signaling
may be critical for CNS myelination. However, precise mechanisms of LINGO |
Project Title: |
Axonal regeneration of CNS neurons after spinal cord injury |
Investigator(s): |
Wu W |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
10/2006 |
Abstract: |
The aim of this study is to examine the effects of combined treatments, lithium chloride (LiC1) plus chondroitinase ABC (ChABC), on neuronal regeneration after spinal cord injury (SCI) and to investigate the potential mechanisms of the treatments. Work programme: by using in vivo model the proposed study will investigate: (1) effects of combined treatment of ChABC and Lithium on axonal regeneration after SCI; (2) potential mechanisms of combined treatments on axonal regeneration after SCI. |
List of Research Outputs |
Chu T.H. and Wu W., Nitric oxide synthase inhibitor attenuates number of regenerating spinal motoneurons in adult rats, NeuroReport. 2006, 17: 969-973. |
Ellis-Behnke
R.G., Liang Y., You S., Tay D.K.C., Zhang S., Wu W., So K.F. and Schneider G.E., Nano Neuro Knitting:
Using Nanotechnology To Repair The CNS, NSTI Nanotech and Bio Nano, |
Ellis-Behnke R.G., Liang Y., Tay D.K.C., Wu W., Schneider G.E., Zhang S. and So K.F., Nano hemostat solution: Immediate hemostasis at the nanoscale, Nanomedicine: Nanotechnology, Biology and Medicine. 2006, 2(4): 207-215. |
Ellis-Behnke R.G., Liang Y., Wu W., You S.W., Schneider G. and So K.F., Nanotechnology and CNS repair, The 5th Asia-Pacific Symposium on Neural Regeneration, Shanghai, December 8-10, 2006, Shanghai, China. 2006, P15/72. |
Fu Q., Wu W., Hu B., Chan S.Y.M., Shao Z., Pepinsky R.B., Mi S. and So K.F., LINGO-1 Antagonists protects retinal ganglion cells in a chronic hypertensive model of glaucoma, the 29th Annual Meeting of the Japan Neuroscience Society, Kyoto, Japan, July 19-21, 2006. S119 No. PS1P-E073. |
Guo J., Zeng Y.S., Liang Y., Wang L., Su H. and Wu W., Cyclosporine affects the proliferation and differentiation of neural stem cells in culture, NeuroReport. 2007, 18(9): 863-868. |
Ji B., Li M., Wu W., Yick L.W., Lee X., Shao Z., Wang J., So K.F., McCoy J.M., Pepinsky R.B., Mi S. and Relton J.K., LINGO-1 antagonist promotes functional recovery and axonal sprouting after spinal cord injuiry, Molecular and Cellular Neuroscience. 2006, 33: 311-320. |
Su H., Chu T.H. and Wu W., Lithium enhances proliferation and neuronal differentiation of neural progenitor cells in vitro and after transplantation into the adult rat spinal cord, Experimental Neurology. 2007, 206: 296-307. |
Yuan Q.J., Scott D.E., So K.F. and Wu W., A subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats, Brain Research. 2007, 1159: 18-27. |
Yuan Q.J., Scott D.E., So K.F. and Wu W., The response of magnocellular neurons of the hypothalamo-neurohyphyseal system to hypophysectomy, nitric oxide synthase expression as well as survival and regeneration in developing vs. adult rats, Brain Research. 2006, 1113: 45-53. |
Zeng Y.S., Nie J.H., Zhang W., Chen S.J. and Wu W., Morphone acts via m-opioid receptors to enhance spinal regeneration and synaptic reconstruction of primary afferent fibers injured by sciatic nerve crush, Brain Research. 2007, 1130: 108-113. |
Researcher
: Xu K |
List of Research Outputs |
Xu K., Ling M.T., Wang X. and Wong Y.C., Evidence of a novel biomarker, s1-Casein, a milk protein, in benign prostatic hyperplasia., Prostate cancer and Prostate Diseases. 2006, 9: 293-297. |
Researcher
: Yau SY |
List of Research Outputs |
Lau W.M., Yau S.Y., Qiu G., Helmeste D.M., Lee T.M.C., Tang S.W. and So K.F., Selective serotonin reuptake inhibitor treatment diminishes inhibition of masculine sexual behavior caused by corticosterone, Thd 4th Congress of Federation of Asian-Oceanian Neuroscience Societies, November 30 - December 2, 2006, Hong Kong.. 2006, 120 No. P-C22. |
List of Research Outputs |
Yeung
S.C., Chiu
K., So K.F. and Chang R.C.C., The effects of
intravitreal injection of IL-10 on the survival of retinal ganglion cells in
the rat glaucoma model, The 5th Asia-Pacific symposium on Neural
Regeneration, |
Researcher
: Yick LW |
Project Title: |
Defining the roles of epidermal growth factor (EGF) in the regulation of chondrocyte differentiation and endochondral bone formation |
Investigator(s): |
Yick LW, Chan SY |
Department: |
Paediatrics & Adolescent Med |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2004 |
Abstract: |
To understand the role of EGF in chondrocyte differentiation using our 'knock-in mice' with targeted expression of EGF in prehypertrophic chondrocytes. |
List of Research Outputs |
Ji B., Li M., Wu W., Yick L.W., Lee X., Shao Z., Wang J., So K.F., McCoy J.M., Pepinsky R.B., Mi S. and Relton J.K., LINGO-1 antagonist promotes functional recovery and axonal sprouting after spinal cord injuiry, Molecular and Cellular Neuroscience. 2006, 33: 311-320. |
Researcher
: Yik SY |
List of Research Outputs |
Yik
S.Y., Ho
Y.S., Lai S.W., So K.F. and Chang R.C.C., Significance of dsRNA
produced by viral infection in neurodegeneration, Second International
Symposium on Healthy Aging: Meeting the Challenges of an Aging Population,
March 3-4, 2007, |
Researcher
: Yip HKF |
Project Title: |
The role of microglia in the survival of retinal ganglion cells (RGCs) following transient retinal ishemia |
Investigator(s): |
Yip HKF, Chang RCC |
Department: |
Anatomy |
Source(s) of Funding: |
Small Project Funding |
Start Date: |
11/2002 |
Abstract: |
To study the effects of: (1) Ischemia on the survival of RGCs and microglial response; (2) Neurotrophic factors (CNTF, BDNF, and bFGF) and cytokines (TGF-[gamma], IL-4, IL-10, TNF-[gamma], CSF-1, and IFN-/LPS) on the survival of RGCs and microglial response after transient retinal ischemia; (3) Neurotrophic factors and cytokines on the microglia from ischemic retina in vitro. |
Project Title: |
In vivo gene transfer of the catalytic subunit of telomerase protects retinal ganglion cells against axotomy-induced cell death |
Investigator(s): |
Yip HKF, Tsao GSW, Chung SK |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
05/2005 |
Abstract: |
To compare TERT mRNA expression levels in axotomized mouse and fish RGCs; to determine whether TERT upregulation protects mouse RGCs from axotomy-induced cell death; to determine signaling pathways involved in the TERT-induced survival of axotomized RGCs. |
Project Title: |
The role of telomerase in protecting retinal ganglion cells against axonal injury |
Investigator(s): |
Yip HKF, Tsao GSW, Leung AYH |
Department: |
Anatomy |
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
Start Date: |
09/2005 |
Abstract: |
To establish the anti-apoptotic role of telomerase in regulating RGC survival after optic nerve (ON) lesion in fish and mice; to examine the possibility that telomerase mediate RGC survival-promoting actions of brain-derived neurotrophic factor (BDNF); to determine possible mechanisms whereby telomerase prevent apoptosis. |
Project Title: |
The interaction of Id2, bHLH and retinoblastoma proteins in the neurogenesis of zebrafish retina |
Investigator(s): |
Yip HKF, Chung SK, Wong YC, Wang X |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
04/2006 |
Abstract: |
During development of the retina,
progenitor cells change their competency over time under the control of
extrinsic (such as neurotrophic factors) and intrinsic regulators (such as
transcription factors). In mouse, retinal progenitors initially proliferate
extensively to increase the cell number but, from embryonic day (E) 10.5
onward, proliferating progenitors start cell differentiation. In the neural
retina, there are six types of neurons and one type of glial cells (Müller
cells). These seven types of cells are differentiated from common progenitors
in an order conserved among many species: ganglion cells first and Müller
cells last. Thus, retinal development consists of three successive processes:
(i) proliferation of progenitors, (ii) neurogenesis, and (iii) gliogenesis.
It has been shown that these processes are controlled by a number of basic
helix-loop-helix (bHLH) genes, which function as intrinsic regulators (1).
Another family of HLH genes, which lack the basic DNA-binding domain, is
known as inhibitors of DNA binding or Id genes. In mammals, there are four
known Id gene family members. Id1, Id2, Id3, and Id4 are expressed in
progenitors of the central nervous system (CNS) in overlapping but
distinctive patterns. The proteins encoded by these genes form inactive
heterodimeric complexes and negatively influences the ability of
tissue-specific bHLH transcription factors to bind to DNA. Thus, Id proteins
function as negative regulators by sequestering cell type-restricted bHLH
transcription factors, and act as a negative regulator of differentiation
(2). Similarly, expression of the mammalian prototype Id gene Id1 is
down-regulated upon differentiation in many cell types, including neurons
(3). Inconsistent with the model mentioned above, however, is the expression
of Id genes in functional, mature cells of several types. For example, Id2
expression is not restricted to early newborn neurons but is also found in
specific neurons throughout the development and adulthood in the mouse brain
(4). Id1, Id2 and Id3 have been shown to interact with cell cycle regulatory
molecules (5). In addition, both genetic and biochemical evidence indicates
that Id2 inhibits retinoblastoma (pRb) protein function to enhance the G1 to
S transition in proliferating cells (6). Together, these data suggest that Id
proteins perform a dual function in the control of cell proliferation and
differentiation. We have provided the first evidence that Id3 protein is
present in the postnatal and adult mouse retina. Id3 are found in the retinal
ganglion cells and amacrine cells (7). The spatial expression pattern of Id2
overlaps and yet is distinct with the pattern of Id3. Id2 expression is
restricted to the amacrine and bipolar cells (8). Basic helix-loop-helix
(bHLH) genes play a key role in the induction of cell type-specific gene
expression. During early development, retinal progenitor cells proliferate in
the ventricular zones and eventually commit to a neuronal fate, progenitor
cells then exit cell cycle and undergo terminal mitosis and at the same time
induce neuron-specific genes. Retinoblastoma tumor suppressor protein (pRb)
has been indicated in this transition. Because pRb plays a role in cell cycle
regulation, inactivation of pRb results in ectopic mitoses and extensive cell
death in the developing nervous system (9). Studies demonstrated a crucial
temporal requirement for the pRb during neuronal phenotypic determination;
these cell cycle regulatory molecules were pivotal for termination of mitosis
and survival of progenitor cells, however, it is not required for the
induction of neurogenesis. The role of pRB and the molecular mechanisms
responsible for coordinately inducing neuronal gene expression are largely
unknown. Interestingly, both positive bHLH and dominant-inhibitory HLH
proteins Id2 are thought to interact with pRb to regulate muscle and brain
development, although it is not known whether similar interactions between
transcription factors and cell cycle proteins regulate neurogenesis in the
retina. Towards this aim, we propose the following objectives: 1. To examine
the expression of endogenous and exogenous Id |
Project Title: |
Bone morphogenetic proteins (BMPs) regulation of the Id gene family in neural progenitors and glial cells of the rat spinal cord following contusion injury |
Investigator(s): |
Yip HKF, Wu W |
Department: |
Anatomy |
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
Start Date: |
04/2007 |
Abstract: |
Injury to the spinal cord leads to a
complex sequence of cellular responses, including axonal degeneration, demyelination,
marcrophage/microglia activation, astrocyte hypertrophy, and neuronal cell
death (1). Reactive astrocytes and activated microglia are histological
hallmarks of central nervous system (CNS) disease or injury. Pronounced
increases in the number of reactive astrocytes form the glial scars at the
lesioned site (2). It is generally believed that failure of axonal
regeneration in the CNS is attributed to the formation of astroglial scars
after injury. In addition, activated astrocytes and reactive marcophates/microglia
can also cause neuronal and oligodendrocyte cell death by releasing
neurotoxins and cytokines (3). This combination of events leads to severe
secondary degeneration in the lesioned spinal cord. Thus, it is thought that
spinal cord regeneration after injury cannot readily occur. Neural
progenitors in the subventricular/ventricular zone of the brain proliferate
and give rise to neurons, astrocytes and oligodendrocytes during CNS
development (4). Recent studies indicate that neural progenitors in adult CNS
retain the ability to proliferate and differentiate (5). Although the
neuronal cell death and activation of astrocyte/microglial after CNS injury
are extensively studied, the response of neural progenitors to CNS injury
remains to be elucidated. Cell fate determination during development and
after injury usually involves transcription factors (6). In the nervous
system, neurogenesis is promoted by proneural basic helix-loop-helix (bHLH)
transcription factors such as NeuroD, neurogenin, and Mash1. These tissue
specific bHLH proteins form heterodimers with E box binding proteins
(E12/E47) and in turn activate gene transcription (7). Functions of bHLH are
negatively regulated by the Id (inhibitor of DNA) family of proteins. Ids,
including Id1-Id4, lack a basic domain (8). Id proteins inhibit neurogenic
bHLH by binding to its heterodimeric bHLH partners and E12/E47 (14). The
expression of Id gene is high in proliferating cells during embryogenesis,
but decrease in differentiating cells in the CNS (9). We have recently
demonstrated that Ids are expressed by differentiated neurons in the adult
mouse retina (10). Id gene family is involved in the regulation of cell
proliferation and differentiation. Studies had shown that Id gene was
expressed in immature and mature astrocytes during development and
upregulated in reactive astrocytes after spinal cord injury (11). These
results suggest that Id genes may play an important role in regulating
astrocyte development and in the formation of reactive astrocytes after CNS
injury; however, little is known about the factors regulating Id expression
in astrocytes. Precise mechanisms by which neurogenesis and gliogenesis are
regulated in the CNS are not fully understood. Embryonic subventricular zone
progenitor cells give rises to the neuronal lineage and glial lineage, which
include astrocytes and oligodendrocytes (12). The fate of neural precursors
in the developing brain is determined by intrinsic cellular programs and by
external cues, such as bone morphogenetic proteins (BMPs) (12). BMPs are
members of the large transforming growth factor-ß (TGF-ß) superfamily. BMPs
have recently emerged as important regulators of nervous system development.
Several lines of evidence have been implicating BMPs in neuronal and glial
development (13). BMPs mediate their effects by heterotetrameric
serine/threonine kinase receptors (BmprI and BmprII) and downstream
transcription factors Smad-1, -5 or -8. The transcription factors are
phosphorylated and form a complex with Smad-4, and the complex is
translocated into the nucleus to activate transcription of specific genes
(14). BMP-2 and BMP-7 are highly expressed in the developing nervous system
(13). It has also been demonstrated that BMP-2 and BMP-7 can induce cortical
neuroepithelial or stem cells to differentiate as astrocytes (15), but the
molecular mechanisms underlying this effect remain largely elusive. BMP
expression in the adult CNS is relatively low, but is dramatically increased
in glial cells and neurons after insult or injury to the CNS (16). The rapid
increase of BMP expression in the CNS after injury is analogous to the
effects of BMP during development, and it may be responsible for the
astrocytic response and gliosis that accompany to many CNS insults. As Smad
binding elements are found on the promoter of Id gene (17), and BMPs have
been shown to regulate Id expression in several cell types, including
embryonic stem cells (17). We therefore propose that the antineurogenic
effect of BMP is induced by a mechanism by which BMP signaling activates Id
genes and in turn suppresses neurogenesis of progenitor cells mediated by
inhibiting the function of neurogenic bHLH, suggesting that injury-induced
increase of BMP expression may regulate glial cell differentiation from progenitor
cells, which are present in the adult spinal cord, and may induce gliosis
after CNS injury. Objectives: 1. To examine whether injury can induce
upregulation of BMP-2, -4, -7 and BmprII expression and to identify
BMP-expressing cells in adult rat spinal cord and in neural stem cells
isolated from adult spinal cord. 2. To examine whether BMP-2, -4 and -7
mediates cell fate alteration from neurogenesis to astrocytogenesis of adult
spinal cord neural stem cells. 3. To examine whether repression of neurogenesis
by BMP-2, -4 and -7 is mediated by Ids. Reference: 1. Young W (1993) J Emerg
Med 11:13-22. 2. Reier PJ et al (1989) In: Seil FJ, ed. Neural regeneration
and transplantation. |
List of Research Outputs |
Du Y.
and Yip H.K.F., Expression
Patterns And Localization Of Dna-binding Protein Inhibitor Id |
Lau
W.M., Tsao G.S.W., So K.F. and Yip H.K.F., Expression Of Telomerase
Reverse Transcriptase In Adult Goldfish Retina, J. Molecular Neuroscience.
|
Niu C. and Yip H.K.F., Neuronal Injury Affects Telomerase Activity In The Adult Rat., The 4th Congress Of Federation Of Asian-oceanian Neuroscience Societies (faons) & Annual Meeting Of The Hong Kong Society Of Neurosciences.. 2006. |
Yip
H.K.F., The Role Of Telomerase In Neuronal
Injury, Second International Symposium On Healthy Aging, "Meeting The
Challenges Of An Aging Population", |
Researcher
: You S |
List of Research Outputs |
Ellis-Behnke
R.G., Liang Y., You S., Tay D.K.C., Zhang S., Wu W., So
K.F. and Schneider G.E., Nano
Neuro Knitting: Using Nanotechnology To Repair The CNS, NSTI Nanotech and
Bio Nano, |
Liang Y., Ellis-Behnke R.G., Tay D.K.C., You S., Schneider G.E. and So K.F., Creation of a more permissive environment using self-assembling peptide nanofiber scaffold in combination with chondroitinase ABC for brain lesion repair and functional return of vision, Society for Neuroscience. 2006, Program No. 720.6. |
So K.F., Liang Y., You S., Tay D.K.C., Schneider G.E. and Ellis-Behnke R.G., Promotion of axonal growth by CNTF in a permissive environment of self-assembling peptide nanofiber scaffold for brain lesion repair and functional return of vision in adult hamsters, Society for Neuroscience. 2006, Program No. 522.14. |
Researcher
: Yu MS |
List of Research Outputs |
Chang R.C.C., Lai S.W. and Yu M.S., Collapse of endoplasmic reticulum as a new mechanism mediating beta-amyloid peptide-triggered neurotoxicity, Alzheimer's and Parkinson's Diseases: Progress and New Perspectives, 8th International Conference AD/PD, Salzburg, Austria, March 14-18, 2007. 4(suppl 1): 61-62. |
Chang R.C.C., Suen A.K.C., Yu M.S., Lai S.W., Cheung Y.T. and Hugon J., Roles of protein translation control in neurodegeneration of Alzheimer's Disease, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientifiic Meeting of The Hong Kong society of Neurosciences, Nov. 30 - Dec. 2, 2006. 7 No. S6. |
Chang R.C.C., Yu M.S. and Lai S.W., Significance of molecular signaling for protein translation control in neurodegenerative diseases, Neurosignals. 2007, 15: 249-258. |
Chang R.C.C., Lai S.W., Yu M.S., Cheung Y.T. and Ho Y.S., The impact from understanding the biological mechanisms of neurodegeneration in Alzheimer's disease to the development of neuroprotective agents, Asian Journal of Gerontology & Geriatrics. 2007, 2(1): 31. |
Chiu K., Ji J., Yu M.S., So K.F. and Chang R.C.C., Activation of microglia/macrophages determines the fate of retinal ganglion cell survival in rat chronic ocular hypertension model, Neurosignals. 2006, 15: 139. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Alkaline extract of lycium barbarum protects against beta-amyloid peptide neurotoxicity in rat cortical neurons by activation of AKT, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 126-127 No. P-C36. |
Ho
Y.S., Yu M.S., So K.F., Yuen W.H. and Chang R.C.C., Attenuation of unfolded
protein responses by reducing stress: an example of neuroprotective effect of
Lycium barbarum, 2006 Hong Kong-Macau Postgraduate Symposium on
Chinese Medicine, August 17, 2006, |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Characterizing the Neuroprotective Effects of Alkaline Extract of Lycium Barbarumon b-amyloid Peptide Neurotoxicity , Brain Research . 2007, 1158: 123-134. |
Ho Y.S., Yu M.S., Lai S.W., So K.F. and Chang R.C.C., Glycoconjugates from anti-aging Lycium Barbarum protect primary cortical neurons from beta-amyloid neurotoxicity, Second International Symposium on Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4, 2007 Hong Kong. 2007, 53 P2. |
Ho Y.S., Yu M.S., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity, Society for Neuroscience. 2006, Program No. 826.10. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Neuroprotective effects of anti-aging Lycium barbarum by a novel extraction method, 2006 World Congress on Chinese Medicine: Charting the Course of Development, Hong Kong, November 23-25, 2006. 247. |
Lai S.W., Yu M.S., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Potential neuroprotective agent from botanical extract: An experience of using Verbena officinalisagainst b-amyloid peptide neurotoxicity, Neurosignals. 2006, 15: 146. |
Yu M.S.,
So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., A new polysaccharide from
nerium indicum elicits neuroprotection against beta-amyloid peptides-induced
apoptosis, Second International Symposium on Healthy Aging: Meeting the
challenges of an Aging Population, March 3-4, 2007, |
Yu M.S., Lai S.W., Suen K.A., So K.F., Hugon J. and Chang R.C.C., Absence of unfolded protein responses in extracellular beta-amyloid peptide-induced neuronal apoptosis, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 129 No. P-C41. |
Yu M.S., Ho Y.S., So K.F., Yuen W.H. and Chang R.C.C., Cytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulum, Neurosignals. 2006, 15: 145. |
Yu M.S., Lai S.W., Suen K.C., Kwok N.S., So K.F., Hugon J. and Chang R.C.C., Extracellular accumulation of beta-amyloid peptides induces apoptosis in cultured neurons via a mechanism independent of unfolded protein responses, Neurosignals. 2006, 15: 141. |
Yu M.S., Molecular mechanisms of neuronal death in beta-amyloid peptide toxicity: from basic science to translational research. 2007, 183 pages. |
Yu M.S., Wong Y.Y., So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., New polysaccharide from Nerium indicum protects neurons via stress kinase signaling pathway, Brain Research. 2007, 1153: 221-230. |
Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Protein kinases as technological platforms to screen neuroprotective agents from chinese medicine, Neurosignals. 2006, 15: 133. |
Yu M.S., Yuen W.H., So K.F. and Chang R.C.C., Significance of neuroprotective polysaccharide from the flowers of Nerium indicum in beta-amyloid peptides neurotoxicity, Society for Neuroscience. 2006, Program No. 826.9. |
Researcher
: Yu MS |
List of Research Outputs |
Chang R.C.C., Lai S.W. and Yu M.S., Collapse of endoplasmic reticulum as a new mechanism mediating beta-amyloid peptide-triggered neurotoxicity, Alzheimer's and Parkinson's Diseases: Progress and New Perspectives, 8th International Conference AD/PD, Salzburg, Austria, March 14-18, 2007. 4(suppl 1): 61-62. |
Chang R.C.C., Suen A.K.C., Yu M.S., Lai S.W., Cheung Y.T. and Hugon J., Roles of protein translation control in neurodegeneration of Alzheimer's Disease, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientifiic Meeting of The Hong Kong society of Neurosciences, Nov. 30 - Dec. 2, 2006. 7 No. S6. |
Chang R.C.C., Yu M.S. and Lai S.W., Significance of molecular signaling for protein translation control in neurodegenerative diseases, Neurosignals. 2007, 15: 249-258. |
Chang R.C.C., Lai S.W., Yu M.S., Cheung Y.T. and Ho Y.S., The impact from understanding the biological mechanisms of neurodegeneration in Alzheimer's disease to the development of neuroprotective agents, Asian Journal of Gerontology & Geriatrics. 2007, 2(1): 31. |
Chiu K., Ji J., Yu M.S., So K.F. and Chang R.C.C., Activation of microglia/macrophages determines the fate of retinal ganglion cell survival in rat chronic ocular hypertension model, Neurosignals. 2006, 15: 139. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Alkaline extract of lycium barbarum protects against beta-amyloid peptide neurotoxicity in rat cortical neurons by activation of AKT, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 126-127 No. P-C36. |
Ho
Y.S., Yu M.S., So K.F., Yuen W.H. and Chang R.C.C., Attenuation of unfolded
protein responses by reducing stress: an example of neuroprotective effect of
Lycium barbarum, 2006 Hong Kong-Macau Postgraduate Symposium on
Chinese Medicine, August 17, 2006, |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Characterizing the Neuroprotective Effects of Alkaline Extract of Lycium Barbarumon b-amyloid Peptide Neurotoxicity , Brain Research . 2007, 1158: 123-134. |
Ho Y.S., Yu M.S., Lai S.W., So K.F. and Chang R.C.C., Glycoconjugates from anti-aging Lycium Barbarum protect primary cortical neurons from beta-amyloid neurotoxicity, Second International Symposium on Healthy Aging: Meeting the Challenges of an Aging Population, March 3-4, 2007 Hong Kong. 2007, 53 P2. |
Ho Y.S., Yu M.S., Lai S.W., Yuen W.H., So K.F. and Chang R.C.C., Neuroprotective effects of alkaline extract of Lycium barbarum on beta-amyloid peptide neurotoxicity, Society for Neuroscience. 2006, Program No. 826.10. |
Ho Y.S., Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Neuroprotective effects of anti-aging Lycium barbarum by a novel extraction method, 2006 World Congress on Chinese Medicine: Charting the Course of Development, Hong Kong, November 23-25, 2006. 247. |
Lai S.W., Yu M.S., Yuen W.H., Zee S.S.Y., So K.F. and Chang R.C.C., Potential neuroprotective agent from botanical extract: An experience of using Verbena officinalisagainst b-amyloid peptide neurotoxicity, Neurosignals. 2006, 15: 146. |
Yu M.S.,
So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., A new polysaccharide from
nerium indicum elicits neuroprotection against beta-amyloid peptides-induced
apoptosis, Second International Symposium on Healthy Aging: Meeting the
challenges of an Aging Population, March 3-4, 2007, |
Yu M.S., Lai S.W., Suen K.A., So K.F., Hugon J. and Chang R.C.C., Absence of unfolded protein responses in extracellular beta-amyloid peptide-induced neuronal apoptosis, The 4th Congress of the Federation of Asian-Oceanian Neuroscience Societies and The 26th Scientific Meeting of The Hong Kong Society of Neurosciences, Nov. 30 - Dec. 2, 2006. 129 No. P-C41. |
Yu M.S., Ho Y.S., So K.F., Yuen W.H. and Chang R.C.C., Cytoprotective effects of Lycium barbarum on cultured neurons against reducing stress on the endoplasmic reticulum, Neurosignals. 2006, 15: 145. |
Yu M.S., Lai S.W., Suen K.C., Kwok N.S., So K.F., Hugon J. and Chang R.C.C., Extracellular accumulation of beta-amyloid peptides induces apoptosis in cultured neurons via a mechanism independent of unfolded protein responses, Neurosignals. 2006, 15: 141. |
Yu M.S., Molecular mechanisms of neuronal death in beta-amyloid peptide toxicity: from basic science to translational research. 2007, 183 pages. |
Yu M.S., Wong Y.Y., So K.F., Fang J.N., Yuen W.H. and Chang R.C.C., New polysaccharide from Nerium indicum protects neurons via stress kinase signaling pathway, Brain Research. 2007, 1153: 221-230. |
Yu M.S., Lai S.W., So K.F., Yuen W.H. and Chang R.C.C., Protein kinases as technological platforms to screen neuroprotective agents from chinese medicine, Neurosignals. 2006, 15: 133. |
Yu M.S., Yuen W.H., So K.F. and Chang R.C.C., Significance of neuroprotective polysaccharide from the flowers of Nerium indicum in beta-amyloid peptides neurotoxicity, Society for Neuroscience. 2006, Program No. 826.9. |
Researcher
: Yuan QJ |
List of Research Outputs |
Yuan Q.J., Scott D.E., So K.F. and Wu W., A subpopulation of reactive astrocytes at affected neuronal perikarya after hypophysectomy in adult rats, Brain Research. 2007, 1159: 18-27. |
Yuan Q.J., Scott D.E., So K.F. and Wu W., The response of magnocellular neurons of the hypothalamo-neurohyphyseal system to hypophysectomy, nitric oxide synthase expression as well as survival and regeneration in developing vs. adult rats, Brain Research. 2006, 1113: 45-53. |
Researcher
: Zhang X |
List of Research Outputs |
Wong
Y.C., Zhang X., Ling M.T. and Wang X., Inactivation of Id-1 gene
induces sensitivity of prostate cancer cells to chemotherapeutic drugs, 5th
International Symposium on Hormonal Carcinogenesis, |
Researcher
: Zhang Z |
List of Research Outputs |
Zhang Z., Xie D., Li X., Wong Y.C., Xin D., Guan X.Y., Chua C.W., Leung S.C., Na Y. and Wang X., Significance of TWIST expression and its association with E-cadherin in bladder cancer., Human Pathology. 2007, 38: 598-606. |
Researcher
: Zhou Y |
List of Research Outputs |
Zhou Y., Bhata I., He Q.Y., Cheung P.T. and Chiu J., Hypoxia-ischemia induces dephosphorylation of collapsin mediator proteins ( CRMPs) in neonatal mice through down regulation of Cdk5/p35, 46th Annual meeting of The American Society for Cell Biology, December 9-13, 2006, San Diego, California, U. S. A.. 2006. |
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