DEPT OF ORTHOPAEDICS AND TRAUMATOLOGY



Researcher : Aladin KDM

List of Research Outputs

Aladin K.D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nanostructure of Collagen Fibrils in Human Nucleus Pulposus and Its Correlation with Macroscale Tissue Mechanics, J. Ortho. Res.. 2009, 28: 497-502.


Researcher : Aladin Kaderbatcha DM

List of Research Outputs

Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nano-structure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics, Journal of Orthopedic Research. 2010, 28: 497-502.
Lu W.W., Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T. and Luk K.D.K., Correlation between the nano-structure and macro-mechanics of human nucleus pulposus, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Zheng Z.M., Yu B.S., Chen H., Aladin Kaderbatcha D.M., Zhang K.B., Zhang J.F., Liu H., Luk K.D.K. and Lu W.W., 251. Effect of iliac screw insertion depth on the stability and strength of lumbo-iliac fixation constructs. An anatomical and biomechanical study, Spine. 2009, 34(16): E565-E572.


Researcher : Borkhuu BB

List of Research Outputs

Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Chan CCY

List of Research Outputs

Choi M.Y., Chan C.C.Y., Chan D., Luk K.D.K., Cheah K.S.E. and Tanner J.A., Biochemical consequences of sedlin mutations that cause spondyloepiphyseal dysplasia tarda, Biochemical Journal. Great Britain, Biochemical Society, 2009, 423: 233-242.


Researcher : Chan CF

List of Research Outputs

Chan C.F., Yuen W.Y.G. and Leung F.K.L., Fixation of distal humeral fracture in elderly patient by locking compression plate Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1285-89, 2009.


Researcher : Chan CW

List of Research Outputs

Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow-derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486-496.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow–derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486–496.
Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.


Researcher : Chan CW

List of Research Outputs

Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow-derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486-496.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow–derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486–496.
Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.


Researcher : Chan KY

List of Research Outputs

Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, Orthopaedic Research Society. 2010.
Chan K.Y., Leung Y.L., Tam V., Lu W.W., Sze K.Y. and Cheung K.M.C., Production of a natural disc scaffold for intervertebral disc tissue engineering, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.


Researcher : Chan KY

List of Research Outputs

Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, Orthopaedic Research Society. 2010.
Chan K.Y., Leung Y.L., Tam V., Lu W.W., Sze K.Y. and Cheung K.M.C., Production of a natural disc scaffold for intervertebral disc tissue engineering, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.


Researcher : Chan YK

List of Research Outputs

Luk K.D.K., Wan T., Wong Y.W., Cheung K.M.C., Chan Y.K., Cheng A., Kwan M., Law K., Lee P.W.H. and Cheing G.L.Y., A multidisciplinary functional restoration program for patients with chronic back pain, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.


Researcher : Chang KC

List of Research Outputs

Chang K.C., Samartzis D., Fuego S.M., Dhatt S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Cheung KMC

Project Title:Setting Up of a Stem Cell Research Programme for Musculoskeletal Tissue Repair
Investigator(s):Cheung KMC, Chan D
Department:Orthopaedics and Traumatology
Source(s) of Funding:The University of Hong Kong Foundation Seed Grant
Start Date:07/2003
Abstract:
To explore a novel method of intervertebral disc repair by implanting autologous MSCs into the degenerated intervertebral disc using a rabbit model.


Project Title:Intervertebral disc regeneration by use of mesenchymal stem cells
Investigator(s):Cheung KMC, Chan D, Wu EX, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2006
Abstract:
(1) What is the fate of the transplanted MSCs within the IVD; (2) at what stage of degeneration is cell therapy most effective; (3) could allogeneic MSCs be used for transplantation; (4) what are the long term effects of transplanted MSCs.


Project Title:Roles and properties of notochordal descendent cells in the intervertebral disc
Investigator(s):Cheung KMC, Chan D, Cheah KSE, Leung YL
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2008
Abstract:
To investigate the distribution and phenotype of the NC cells in infant, adolescent, adult, and aged IVD; to investigate the fate and function of NC cells during disc degeneration and MSC-induced repair.


Project Title:Plasma ion implanted anti-bacterial surface coating for orthopaedic metallic implants
Investigator(s):Cheung KMC, Kao RYT, Yang D
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2008
Completion Date:12/2009
Abstract:
To develop an anti-bacterial surface coating using plasma immersion ion implantation technology for all orthopaedic metallic implants. Seed funding is sought to perform a pilot study including surface mechanical and biological testing in order to demonstrate the feasibility of the anti-bacterial ability in vitro. Metallic materials continue to play an essential role as biomaterials in repairing or replacement of bone tissues that have become diseased or damaged [1]. Titanium Ti-6Al-4V alloy is widely applied because of its high mechanical strength, corrosion resistance and superior fatigue properties as well as biocompatibility [2]. However, debridement of wound or implant due to bacterial infection is sometime seen in bone fracture fixation and deformity correction. Bacterial adhersion on the implant surface is considered to be an important event in the pathogenesis of bacterial infection. The mechanism involves non-specific and specific adherence. The nonspecific one is thought to involve nonspecific processes mediated by physico-chemical interactions such as hydrophobic interactions and electrostatic interactions, whereas the specific one is mediated by extracellular polysaccharides and lectin-like substances [3]. To avoid bacterial infection, inhibition of initial adhesion of bacteria on the implant surface is essential. An implant surface with anti-bacterial activity is an advantage [4]. Antibiotic coating is recently used to inhibit bacterial adhesion. Local delivery of antibiotics is required to avoid systemic side effects of parenteral antibiotics. Also, local delivery can yield higher concentrations in the relevant tissues, thereby improving efficacy and possibly reducing the necessary duration of treatment. Since the majority of orthopaedic implant device-related infections are Staphylococci strain, the major choice of antibiotics loaded on implant surface is vancomycin using sol-gel film formation [7]. However, the properties of vancomycin containing sol-gel films extremely rely on several processing parameters such as the composition of sol gel, speed of withdrawal, and number of loaded layers. The total amount and release rates of loaded antibiotics increase along with number of layers. Although the release of such antibiotics can be controlled by those parameters, the study suggests that the concentration of vancomycin exceeds the minimal inhibitory concentration (MIC) of vancomycin against bacteria (S. sureus) [7]. Therefore, the release rate of drug-containing films has to be controlled carefully [7-9]. However, the optimization process in sol-gel technique is difficult. Excess release may spoil the normal function of the peripheral cells. Apart from sol-gel technology, antibiotics can be also loaded to the orthopaedic implant surface by an alternative method so as to render the surface with bactericidal function. With the use of solid phase peptide synthesis, the antibiotic can be bonded to aminopropylated titanium implant surface [10]. This solid phase antibiotic is able to retain the activity of the soluble antibiotic and prevent biofilm formation at the site of the implant. The most critical issue is that the titanium-bonded antibiotics will not lose from the surface during bacteria incubation [10]. However, the peptide synthesis is an expensive process. This will undoubtedly add costs to manufacturing and health care system eventually. In addition to the limited yield, the peptide coating also becomes unstable so that it is not allowed for long term storage. Other previous publications revealed that the surface antibacterial property of implantable material is varied by surface chemistry and morphology [5-6]. The bacteria can actively proliferate when the surface roughness is high. Therefore, the modifications of surface properties such as topography and chemistry using chemical vapor deposition (CVD), physical vapor deposition (PVD), plasma ion implantation and laser-assisted treatments are the alternative in addition to antibiotics coating. Plasma Immersion Ion Implantation (PIII) was first introduced in the early 1980’s. This burgeoning technology has found many applications, ranging from the enhancement of aerospace and industrial components to the fabrication of new materials used in the microelectronics and biomedical industry. This surface coating technique has a number of advantages such as simplicity, low cost, efficiency, large coating area and batch processing [6]. The use of this advanced technology in biomaterials has been widely investigated [7]. The preliminary result of our recent study suggests that the medical grade titanium alloy implanted with oxygen using plasma immersion ion implantation technology can significantly reduce the colony formation of bacteria. After PIII treatment, a layer of titanium oxide has been formed on the surface. This modified surface demonstrates a significant reduction of the presence of bacteria, thereby minimizing the possibility of post-operative inflammation. The preliminary work has demonstrated the feasibility of the use of oxygen PIII in reducing the bacterial adhesion and proliferation. However, the science of this novel antibacterial property using PIII has not yet been studied. Therefore, the aim of the present study is to investigate the correlation between antibacterial capability of plasma engineered surface and bacterial adhesion. If successful, we could establish a new antibacterial method by using a convenient and economical technology. The reason to apply for seed funding is to study which surface parameters such as roughness and chemistry of plasma treated medical grade metallic materials may achieve superior antibacterial function. Firstly, we aim at implanting oxygen plasma to the material surface at various implantation voltages. This treatment may help achieve various surface roughnesses. Secondly, other elements such as carbon will also be implanted in order to obtain various chemical compositions on the surface. After PIII treatment simple bacterial cultures will be used to evaluate the antibacterial ability of plasma-treated metallic materials. Once the results are available, we will submit a more elaborate application to RGC for a comprehensive study on the use of plasma-treated antibacterial metallic medical materials for clinical use. We believe that the success of the external grant applications can be significantly increased if some preliminary results are available. # Please refer to Appendix I for the list of references.


Project Title:Uncovering new compounds and mechanisms for treatment of intervertebral disc degeneration by Chemical Genetics
Investigator(s):Cheung KMC, Chan D, Kao RYT, Leung YL
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2009
Abstract:
(1) Perform a secondary screen on a smaller number of compounds to precisely identify the most potent ones; (2) Perform a tertiary screen in human disc cells and chondrocytes; (3) Examine the effect of the potent compounds from Objective 2 on proteoglycan metabolism; (4) Study the potent compounds from Objective 3 using an established rabbit model of disc degeneration.


Project Title:Development of an Intervertebral disc organ culture with human nucleus pulposus cells and xenogeneic scaffold
Investigator(s):Cheung KMC, Leung YL
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2009
Completion Date:05/2010
Abstract:
Introduction Low back pain is common and may cause severe disability. It is one of the most frequent reasons for clinic visits and loss of work hours, leading to considerable expenditure in treatment and wage compensation. Although low back pain is complex in aetiology, it is associated with degeneration of the intervertebral disc (IVD). IVDs are avascular fibrocartilagenous structures between the vertebral bodies of the spine that functions as a joint and allows spinal motion. The IVD consists of three components: an outer fibrous ring (annulus fibrosus, AF), an inner gelatinous filling (nucleus pulposus, NP), and the upper and lower cartilaginous endplates (EP) which sandwich the former two structures. The specific cell phenotype of IVD cells has not yet been clearly characterized, but the in general the IVD is populated by three major cell types: chondrocytes in EP, vacuolated notochordal cells and chondrocyte-like cells in the NP, and fibroblast-like cells in the AF. The NP and AF contain abundant extracellular matrix, particularly collagens and proteoglycans27. In humans, the NP loses notochordal cells and is dominated by chondrocyte-like cells during IVD maturation10,11. The mature IVD is thought to be similar to hyaline cartilage in that it expresses phenotypic markers of chondrocytes such as collagen II, collagen IX, aggrecan, and SOX98,19,23,31. It is generally accepted that these NP cells are similar, but not the same, as chondrocytes and their exact origin and phenotype are not clear. IVD degeneration involves changes in disc morphology and composition of extracellular matrix. It is believed that the process of degeneration begins in the NP with a loss of proteoglycans and water. The consequential loss of shock-absorbing capacity in the degenerated NP has been speculated to cause the structural failure of IVD. Current methods of treating severe back pain due to IVD degeneration involve surgical fusion of the spine. While this is successful in treating the pain, it renders the motion segment immobile and is non-physiological. Methods of treating disc degeneration, such as prosthetic IVD replacements22 and IVD transplantation20, have been developed in an attempt to preserve segment motion. Various biological therapies under investigation for treating IVD degeneration aim to preserve or restore motion of the affected spinal segment though regenerating disc cells and stimulating their cellular function. Considerable effort is currently placed on biological therapies including direct injection of growth factors16, their introduction via gene therapies26, and nucleus pulposus and stem cell transplantation3,9,15. By high-throughput screening of chemical libraries, our group has shown that small compounds may also have a potential in the stimulation of proteoglycan production in chondrocytes and possibly have similar action on IVD cells25. Key Issues and Problems The majority of the biological therapies are currently being tested in animal models which is an important step before clinical trials. To date, however, all animal models for IVD degeneration suffer from deficiencies (reviewed in reference Alini et al.1). This is partly due to the unclear aetiology of human IVD degeneration and the different cellular makeup, geometry and mechanical environment of animal IVDs from that of humans. While large animal models are more relevant in spine biomechanics, up-keeping the animals are expensive and histological analyses are difficult. Small animal models such as rodents have more favourable molecular tools, but their IVDs are small and difficult to handle, and their cellar makeup is largely different from that of adult humans. In addition, the uncertainties in the nature and phenotype of nucleus pulposus (NP) cells in normal and degenerated human IVD pose difficulties in generation and testing of hypotheses in the animal models. Given the above limitations and uncertainties, study on human IVD would have advantages over the animal models and be an important alternative for testing the biologic therapies. To this end, creating an in vitro whole organ human disc culture model would be ideal. However, it is unlikely that one could secure large numbers of whole human IVD of equivalent quality and degeneration status for multiple testing. The use of human IVD cells would be more practical, but primary cells under in vitro culture conditions are prone to lose their phenotype, particularly in monolayer cultures. While 3D cultures in a synthetic scaffold may possibly overcome the problem, they still differ from an intact IVD in their micro- and mechanical environment, and demonstration of the disc cell phenotype in the scaffold is limited. Purpose of this proposal Because anatomical and cellular dissimilarities exist between animal and human IVD, a human IVD model is needed to better understand disc cell biology, the pathophysiology of degeneration, and for testing potential regenerative therapies. Culturing whole human IVD explants in a bioreactor would appear to be the most appropriate, but access to whole fresh human IVD is not practical owing to limited availability of cadaveric specimens. Human IVD cells may be more readily available via surgical or cadaveric retrieval. Our group has developed a mechanically loaded organ culture system for whole IVD (IVD bioreactor system). We hypothesize that human NP cells can be implanted into a decellularized xenogeneic IVD in our bioreactor system, and able to maintain their viability and native phenotype after the culture, thereby providing a more realistic human model to test different biologic therapies. This proposal aims to validate and optimize the setup of this culture system, and to obtain data to evaluate its validity through a pilot short-term culture investigation. We target to make use of our experience in decellularization of intervertebral discs, our existing methodology on human IVD gene expression profiling, our established IVD bioreactor system, and our ample clinical IVD specimens, to develop such an in vitro human NP cell organ culture model. In this study, the efficiency of IVD decellularization technique and the viability of the human NP cells implanted in the proposed culture system will be first examined. The hypothesis will then be preliminarily tested by comparing the gene expression among the human NP cells cultured in the system, with NP cells cultured using current 3D culture techniques and uncultured NP cells from non-degenerated human IVD. Objectives 1) To determine the efficiency of decellularization in IVD 2) To determine the viability of human NP cells cultured in xenogeneic IVD within the bioreactor system. 3) To compare human NP cells cultured in IVD bioreactor system, with NP cells cultured in 3D-scaffold and uncultured NP cells by gene expression profiling.


Project Title:Molecular Analysis of the BMP-2 Action on Intervertebral Disc Cells
Investigator(s):Cheung KMC, Chan D, Zhou G
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:10/2009
Abstract:
1) To comparatively analyze the role of BMP-2 and BMP-7 in regulating the major functional properties of AF and NP cells of healthy and degenerative IVD; 2) To elucidate the signaling mechanisms underlying the role of BMP-2 and BMP-7 in NP and AF cells, with the emphasis on exploring feasible approaches to intensifying BMP signaling and potentiating BMP action.


Project Title:Cardiovascular risk factors associated with intervertebral disc degeneration/low back pain
Investigator(s):Cheung KMC, Samartzis D, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Health and Health Services Research Fund - Full Grants
Start Date:01/2010
Abstract:
As part of the largest radiographic and genetic-based study in the world assessing DDD and associated symptoms, we will attempt to address the role of cardiovascular risk factors associated with DDD.


List of Research Outputs

Aladin K.D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nanostructure of Collagen Fibrils in Human Nucleus Pulposus and Its Correlation with Macroscale Tissue Mechanics, J. Ortho. Res.. 2009, 28: 497-502.
Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nano-structure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics, Journal of Orthopedic Research. 2010, 28: 497-502.
Chan B.P., Cheng H.W., Chik T.K., Cheung K.M.C. and Luk K.D.K., Methods for Complex Tissue Engineering, Provisional Patent Application (filed on 15 June 2010) (Application No. 61/354,869). 2010.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow-derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486-496.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow–derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486–496.
Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, Orthopaedic Research Society. 2010.
Chan K.Y., Leung Y.L., Tam V., Lu W.W., Sze K.Y. and Cheung K.M.C., Production of a natural disc scaffold for intervertebral disc tissue engineering, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Chan Q., Kim M., Anthony M., Cheung K.M.C., Chan A., Chan T. and Khong P.L., Quantitative comparison of T1r with T2 in intervertebral disc in vivo at 3T, Joint ISMRM-ESMRMB Annual Scientific Meeting, Stockholm, Sweden, 1-7 May . 2010.
Chan Q., Anthony M., Zhang Z., Cheung K.M.C. and Kim M., Quantitative evaluation of diffusion tensor imaging at 3T in the human lumbar intervertebral disc degeneration , Joint ISMRM-ESMRMB Annual Scientific Meeting, Stockholm, Sweden, 1-7 May . 2010.
Chang K.C., Samartzis D., Luk K.D.K. and Cheung K.M.C., Cervical spine disease in Asian populations, Hong Kong Medical Journal. 2010, 16: 69-70.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S.S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Cheah K.S.E., Au Y.K., Szeto Y.Y., Wynn S., Chan Y.S., Cheung K.M.C., Chan W.Y., Lovell-Badge R.H., Chan D. and Fritzsch B., Molecular and developmental insights into the pathogenesis of the SOX9Y440Xcampomelic dysplasia mutation, 16th International Society of Developmental Biologists Congress 6-10th September 2009 Edinburgh International Conference Centre, UK . 2009.
Cheng H.W., Tsui Y.K., Cheung K.M.C., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres - a 3D model to study the effects of acellular matrix on stem cell fate, Tissue Engineering Part C Methods . 2009, 15(4): 697-706.
Cheng H.W., Tsui Y.K., Cheung K.M.C., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres. , WACBE (World Association for Chinese Biomedical Engineers) World Congress on Bioengineering 2009. 26-29 Jul 2009, Hong Kong, China. . 2009, 58.
Cheung K.M.C., Biologic motion preservation, Argentinian Spine Society Meeting, Buenos Aires, Argentina, September 4-5, 2009.
Cheung K.M.C., Case presentations on lumbar disc pathologies , AOSpine Course on Disc Pathology, Buenos Aires, Argentina, September 3, 2009.
Cheung K.M.C., Cement augmentation of pedicle screws, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Cheung K.M.C., Clinical examination in myelopathy , Argentinian Spine Society Meeting, Buenos Aires, Argentina, September 4-5, 2009.
Cheung K.M.C., Degeneration and the aging spine, AOSpine Global Interactive-Advanced Course, Kuala Lumpur, Malaysia, October 26-30, 2009.
Cheung K.M.C., Yeung K.W.K., Lu W.W. and Chung C.Y., Device for correcting spinal deformities, Japan patent no. 4423197, granted on December 11, 2009, 2009.
Cheung K.M.C., Editorial Board Member for International Journal of Artificial Organs (2009-2011). 2009.
Cheung K.M.C., Editorial Board of Orthopaedic Journal of China (2009-2012). 2009.
Cheung K.M.C., En-bloc resection of spinal tumours , Argentinian Spine Society Meeting, Buenos Aires, Argentina, September 4-5, 2009.
Cheung K.M.C., Etiology and pathology of disc degeneration , AOSpine Course on Disc Pathology, Buenos Aires, Argentina, September 3, 2009 . 2009.
Cheung K.M.C., Yeung K.W.K., Lu W.W., Poon T.L. and Chow S.P., Fixation device, US Regular patent no. 7,695,471, granted on April 13, 2010, 2010.
Cheung K.M.C., Fixation strategies in osteoporosis , Argentinian Spine Society Meeting, Buenos Aires, Argentina, September 4-5, 2009.
Cheung K.M.C., Fusion level determination in AIS , Argentinian Spine Society Meeting, Buenos Aires, Argentina, September 4-5, 2009.
Cheung K.M.C., Genetics of IVD degeneration , AOSpine Course on Disc Pathology, Buenos Aires, Argentina, September 3, 2009.
Cheung K.M.C., How many screws are needed for AIS , 2nd Spinal Defomrity Study Group (China Section) meeting, Nanjing, PR China, October 9-11, 2009.
Cheung K.M.C., Instrumentation strategies in scoliosis correction , Argentinian Spine Society Meeting, Buenos Aires, Argentina, September 4-5, 2009.
Cheung K.M.C., International Advisory Board of Journal of Orthopaedic, Trauma and Rehabilitation since 2010. 2010.
Cheung K.M.C., Management of TB kyphosis, 3rd China International Congress of Orthopaedics, Beijing, PR China, August 6-9, 2009.
Cheung K.M.C., Management of black disc in lumbar spine , AOSpine Global Interactive-College Course, Kuala Lumpur, Malaysia, October 26-30, 2009.
Cheung K.M.C., Management osteoporotic fracture with spinal deformity , HA Commissioned Training in Adult Spinal Deformity, Hong Kong, November 26-27, 2009.
Cheung K.M.C., Non-PMMA cement for vertebroplasty and kyphoplasty, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Cheung K.M.C., Overview of biological motion preservation , AOSpine Global Interactive-College Course, Kuala Lumpur, Malaysia, October 26-30, 2009.
Cheung K.M.C., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Cheung K.M.C., Samartzis D., Natarajan D., Li J., Cheung W.Y., Lenke L.G. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Cheung K.M.C., Posterior techniques in scoliosis correction, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in scoliosis correction with alternate level pedicle screw fixation, Journal of Bone and Joint Surgery. 2010, 92: 169-176.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation., 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.. 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, J Bone Joint Surg (Am). 2010, 92: 169-76.
Cheung K.M.C., Regional Editor of Evidence-based Spine-Care Journal since 2010. 2010.
Cheung K.M.C., Mok P.S., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl's nodes of the lumbar spine: prevalence, classification, associated risk factors and clinical relevance, 83rd Annual Meeting of the Japanese Orthopaedic Association, Tokyo, Japan, May 27-30, 2010.
Cheung K.M.C., Samartzis D., Karppinen J., Mok P.S., Ho D.W.H., Fong D.Y.T. and Luk K.D.K., “Skipped” level disc degeneration of the lumbar spine, a MRI study of 1989 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., In-vivo demonstration of spontaneous vertebral derotation in thoracic adolescent idiopathic scoliosis with fulcrum bending radiograph, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Chik T.K., Luk K.D.K., Cheung K.M.C. and Chan B.P., Creation of a tissue interface between bone-like construct and soft tissue-like construct. , TERMIS 2nd World Congress, Seoul, Korea, Aug 31- 3 Sep 2009. S211: 802.
Duong L., Cheriet F., Labelle H., Cheung K.M.C., Abel M.F., Newton P.O., McCall R.E., Lenke L.E. and Stoke I.A.F., Inter- and intra-observer variability in the identification of the Lenke classification lumbar modifier in adolescent idiopathic scoliosis, Journal of Spinal Disorders and Techniques. 2009.
Fok A., Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The prevalence, factors, and clinical profile associated with Tarlov cysts in Southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Fok A.W.M., Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The prevalence, factors, and clinical profile associated with tarlov cysts in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Fong D.Y.T., Lee C.F., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., A meta-analysis of the clinical effectiveness of school scoliosis screening, Spine. 2010, 35(10): 1061-1071.
Fong D.Y.T., Lee C.F., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., A meta-analysis of the clinical effectiveness of school scoliosis screening, Spine (Phila Pa 1976). 2010, 35(10): 1061-1071.
Garcia-Barcelo M.M., Yeung M.Y., Miao X.P., Tang S.M., Chen G., So M.T., Ngan E.S.W., Lui V.C.H., Chen Y., Liu X., Hui K.J.W.S., Li L., Guo W.H., Sun X.B., Tou J.F., Chan K.W., Wu X.Z., Song Y., Chan D., Cheung K.M.C., Chung P.H.Y., Wong K.K.Y., Sham P.C., Cherny S.S. and Tam P.K.H., Genome-wide association study identifies a susceptibility locus for biliary atresia on 10q24.2, Human Molecular Genetics. 2010, 19 (14): 2917-2925.
Guo J.J., Luk K.D.K., Karppinen J., Yang H.L. and Cheung K.M.C., Prevalence, distribution, and morphology of ossification of the ligamentum flavum, Spine. 2010, 35(1): 51-56.
Kao Y.P.P., Chan D., Cheah K.S.E., Cheung K.M.C., Ho D.W.H., Karppinen J., Leong J.C.Y., Yip S.P., Song Y. and Sham P.C., Genome-wide Association Study of Degenerative Disc Disease (DDD), 14th Research Postgraduate Symposium, December 2 & 3, 2009, The University of Hong Kong. 2009.
Kim M., Chan Q., Anthony M., Cheung K.M.C., Samartzis D., Chan T. and Khong P.L., Assessment of glycosaminoglycan distribution in human lumbar intervertebral discs using chemical exchange saturation transfer, Joint ISMRM-ESMRMB Annual Scientific Meeting, Stockholm, Sweden, 1-7 May . 2010.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Spine Chapter's Most Promising Spine Paper Award. Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Lee C.F., Fong D.Y.T., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Mak K.H., Yip P.S.F. and Luk K.D.K., A meta-analysis of the clinical effectiveness of school scoliosis screening, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lee C.F., Fong D.Y.T., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., Costs of School Scoliosis Screening: A Large, Population-Based Study, Spine (Phila Pa 1976). 2010, Epub ahead of print.
Lee K.S., Lam T.K., Song Y., Cheah K.S.E., Cheung K.M.C. and Chan D., In vivo study of Asporin, a genetic risk factor for Osteoarthritis and degenerative disc disease, 2010 Hong Kong Inter-University Biochemistry Postgraduate Symposium, CUHK, Hong Kong, 15 May,. 2010.
Leung H.B., Cheung K.M.C., Lu W.W., Tseng H. and Luk K.D.K., The effect of insertion timing on pullout strength of pedicle screws from polymethylmethacrylate bone cement, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Leung Y., Natarajan D., Samartzis D., Borkhuu B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis (AIS) using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Leung Y.L., Tsui Y.K., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 55th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA. 2010.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Li J., Cheung K.M.C., Samartzis D., Zhu X., Li M. and Luk K.D.K., Key –Vertebral Screw Strategy (KVSS): an alternative surgical strategy for main thoracic curve correction in patients with adolescent idiopathic scoliosis, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Li J., Cheung K.M.C., Samartzis D., Zhu X.D., Li M. and Luk K.D.K., Key-Vertebral Screw Strategy (KVSS): an alternative surgical strategy for main thoracic curve correction in patients with adolescent idiopathic scoliosis, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009 . 2009.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Canada patent no. 2,395,910, granted on September 22, 2009, 2009.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Hong Kong Standard patent no. HK1046378, granted on July 17, 2009, 2009.
Li Y.Y., Cheng H.W., Wong M.Y., Cheung K.M.C., Chan D. and Chan B.P., Collagen-mesenchymal stem cell microspheres for cartilage tissue engineering. , TERMIS 2nd World Congress, Seoul, Korea, Aug 31- 3 Sep 2009. . 2009, S69:222.
Li Y.Y., Cheng H.W., Chan P.M., Wong M.Y., Teng W.K., Chow S.T., Cheung K.M.C., Chan D. and Chan B.P., Repair of osteochondral defects with collagen-mesenchymal stem cell microspheres in a rabbit model. , ISSCR 8th Annual Meeting. Jun 16-19, 2010, Moscone West, San Francisco, CA USA. . 2010, #W-50, p250.
Liao H., Huang S., Cheung K.M.C., Chan D., Luk K.D.K. and Zhou G., Functional characterization of miRNA125b, miRNA140 and miRNA199a in chondrogenetic differentiation of mesenchymal stem cells, Cell Adhesion & Communication.. 2009, In press.
Lu W.W., Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T. and Luk K.D.K., Correlation between the nano-structure and macro-mechanics of human nucleus pulposus, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of PEEK Bioactivity by Plasma Surface Modification., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug-3Sep 2009. Poster presentation.. 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of Osteoblast Attachment and Growth on PEEK by Water and Ammonia Plasma Surface Treatment. (6-9 Mar 2010), 56th Annual Meeting of the Orthopaedic Research Society, New Orleans. 2010, Paper 1193.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of osteoblast attachment and growth on PEEK by water and ammonia plasma surface treatment, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Lui S.C., Yeung K.W.K., Chu P.K., Kwok T.K., Pan H., Luk K.D.K. and Cheung K.M.C., Plasma-modified polyetheretherketone for orthopaedic applications: bulk and surface mechanical characterisations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Lui S.C., Cheung K.M.C. and Yeung K.W.K., Surface bioactivity enhancement of polyetheretherketone by plasma immersion ion implantation, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., The Effect of Plasma Technology in Bioactivity Enhancement of PEEK. , 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep2009. Poster presentation.. 2009.
Luk K.D.K., Wan T., Wong Y.W., Cheung K.M.C., Chan Y.K., Cheng A., Kwan M., Law K., Lee P.W.H. and Cheing G.L.Y., A multidisciplinary functional restoration program for patients with chronic back pain, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Luk K.D.K., Vidyadhara S., Lu D.S., Wong Y.W., Cheung W.Y. and Cheung K.M.C., Coupling between sagittal and frontal plane deformity correction in idiopathic thoracic scoliosis and its relationship with post-operative sagittal alignment, Spine. 2010, 35(11): 1158-1164.
Mok F., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., 2010 ISSLS Prize Winner: Prevalence, determinants, and association of Schmorl's nodes of the lumbar spine with disc degeneration: a population-based study of 2,449 individuals, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Mok F., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in Southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Mok F., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in Southern Chinese, 4th International Congres of Chinese Orthopaedic Association. Xiamen, China, 2009.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., ISSLS Prize for Lumbar Spine Research. Prevalence, determinants and association of Schmorl’s Nodes of the lumbar spine with disc degeneration: A population-based study of 2499 individuals., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010. 2010.
Mok P.S., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., Prevalence, determinants and association of Schmorl’s nodes of the lumbar spine with disc degeneration: a population-based study of 2449 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Mok P.S., Cheung K.M.C., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl’s nodes of the lumbar spine: prevalence, classification, associated risk factors, and clinical relevance, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Best Clinical Paper Award for Associate Member. Is there a relationship between intervertebral disc degeneration based on MRI and low back pain?, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 4th International Congress of the Chinese Orthopaedic Association. Xiamen, China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the Orthopaedic Research Society. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Determinants of lumbar disc degeneration in older individuals, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Determinants of lumbar disc degeneration in older individuals, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on MRI and low back pain? Best Clinical Paper Award, Hong Kong Orthopaedic Association. 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on MRI and low back pain?, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on magnetic resonance imaging and low back pain, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS). Most Promising Spine Paper Award, Hong Kong Orthopaedic Association. 2009.
Samartzis D., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., Prevalence, determinants, and association of Schmorl's nodes of the lumbar spine with disc degeneration: a population-based study of 2,449 individuals. Best Clinical Study Prize, International Society for the Study of the Lumbar Spine. 2010.
Samartzis D., Karppinen J., Mok P.S., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Skipped level disc degeneration of the lumbar spine, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Samartzis D., Karppinen J., Mok P.S., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Skipped level disc degeneration of the lumbar spine, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 4th International Congress of the Chinese Orthopaedic Association. Xiamen, China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between concomitant cervical and lumbar disc degeneration and associated abnormalities, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between concomitant cervical and lumbar disc degeneration and associated abnormalities, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between overweight / obesity and low back pain in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between overweight/obesity and low back pain in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the Orthopaedic Research Society. New Orleans, Louisiana, USA, 2010.
Saw L.B., Cheung K.M.C., Grozman S.A.M. and Luk K.D.K., A practical classification of the distal radius and ulna radiograph for assessing skeletal maturity, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Sze K.L., Nagy A.N.D.R.A.S., Fosang A.M.A.N.D.A., McCulloch D.A.N.I.E.L., Cheung K.M.C. and Chan D., Poster Presentation: Impact Of Activating Adamts-5 In Cartilage-related Degenerative Disorders , UGC AoE Site Visit . 2010.
Tong W.Y., Yip K., Mo S., Cheung K.M.C., Luk K.D.K. and Yeung K.W.K., Promotion of bone formation at implant interface by local injection of mouse bone marrow-derived mesenchymal stem cells, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Tong W.Y.T., Liang Y.M., Tam V., Yip H.K., Kao R.Y.T., Cheung K.M.C., Yeung K.W.K. and Lam Y.W., Biochemical characterization of the cell-biomaterial interface by quantitative proteomics, Mol Cell Proteomics. 2010.
Tsang K.Y., Cheung K.M.C., Chan D. and Cheah K.S.E., The developmental roles of the extracellular matrix: beyond structure to regulation, Cell Tissue Res. 2010, 339(1): 93-110.
Tsui Y.K., Leung Y.L., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Tsui Y.K., Cheung K.M.C., Leung Y.L., Chan D. and Kao R.Y.T., Uncovering new compounds for treatment of interveretebral disc degeneration by chemical genetics, 2010.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A Biodegradable Polymer-Based Coating To Control The Performance Of Magnesium Alloy Orthopaedic Implants, Biomaterials. 2010, 31(8): 2084-209.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., A New PCL Based Porous Membrane to Control the Degradation of Biodegradable Metallic Materials., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug–3Sep 2009. Poster presentation.. 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants biomaterials, Biomaterials. 2010, 31: 2084-2096.
Wong H.M., Yeung K.W.K., Leung Y.L., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., In-vitro analysis of gene expressions of osteoblastic cells while exposing to various concentrations of magnesium ions, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., Reduction of the Corrosion Rate of Biodegradable Metallic Materials by Al2O3 PIII surface Treatment., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24pril2010.. 2010, 868.
Wong Y.W., Cheung K.M.C., Cheung W.Y. and Luk K.D.K., The importance of spinal cord monitoring before and after turning the patients prone for operations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., 250. Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009, 17: 1959-1966.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of ninety-one cases, Spine. 2010, 35(6): 635-641.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yee F.Y.A., Cheung K.M.C. and Chan D., Transcriptome and proteome of the intervertebral disc in health and disease, 2010.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, Spine. 2010, 35: 635-641.
Yeung C.Y., Leung K.Y., Yeung K.W.K., Kao R.Y.T., Chu P.K., Cheung K.M.C. and Luk K.D.K., Inhibition of metallic implant-related bacterial infections by novel water plasma surface treatment, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Tu H., Chu P.K. and Liu X.Y., Alterations of Surface Mechanics of Nickel Titanium Shape Memory Alloys after Plasma Surface Treatment. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010).. 2010, 110-111.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. PCT Patent Application (No.: PCT/CN2010/000216) Filed: Feb 18, 2010., PCT Patent Application. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010., U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010.. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, PCT Patent Application (No.: PCT/CN2010/000216) filed 18 February 2010. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, U.S. Non-Provisional Patent (No.: 12/706,483). filed 16 February 2010. 2010.
Yeung K.W.K., Lai D.W.T., Lai D.W.T., Lai D.W.T., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Wu S.L., Tu H., Chu P.K. and Chung C.Y., Biomechanical Analysis of Plasma Treated NiTi Materials for Spinal Deformity Correction., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May 2010). 2010, 24-25.
Yeung K.W.K., Lui S.C., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of Cell Adhesion and Proliferation on Polyetheretherketone by Using Plasma Surface Modification., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24April2010.. 2010, 864.
Yeung K.W.K., Leung K.Y., Kao R.Y.T., Chu P.K., Yip K., Luk K.D.K. and Cheung K.M.C., Feasibility Study of a New Plasma Coating to Suppress Bacterial Adhesion in Orthopaedic, Proceedings 4th International Conference on Technological Advances of Thin Films and Surface Coatings (ThinFilms 2008) (July 13-16, 2008). 2009, (Paper 4150) 35.
Yeung K.W.K., Lam K.O., Luk K.D.K., Cheung K.M.C., Wu S.L., Hu T., Liu X.Y., Chu C.L., Chu P.K. and Chan D., Long Term Cytocompatibility and In vivo Investigation of Nitrogen Plasma Implanted Shape Memory Alloy. , International Conference on Shape Memory ands Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 104-105.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K. and Chung C.Y., Metallic Implants for Spinal Deformity Correction, Advances in Biomedical Sciences and Engineering. USA, Bentham Science Publisher Ltd, 2009, 1-44.
Yeung K.W.K., Lam W.N., Natarajan D., Wu S.L., Hu T., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Nickel Ion Level in Scoliotic Patients Implanted with Nitrogen Plasma Surface Modified Nickel-Titanium Superelastic Spinal Implant., 3rd IEEE International NanoElectronics Conference (INEC), Hong Kong, 3-8 January 2010. Oral presentation.. 2010, Paper BC410, p. 134.
Yeung K.W.K., Kao R.Y.T., Chu P., Luk K.D.K. and Cheung K.M.C., Oxygen Plasma Implanted Titanium Surface to Resist Staphylococcus Aureus Adhesion., 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep 2009. Oral presentation.. 2009.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Wu S.L., Liu X.Y., Chu C.L., Chung C.Y. and Chu P., Plasma Surface Modification of Nickel Titanium Shape Memory Alloys, Advances in Biomedical Sciences and Engineering. USA, Bentham Science Publisher Limited, 2009, 45-68.
Yeung K.W.K., Lam W.N., Wu S.L., Tu H., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Post-Operative Monitoring of Nickel Ion Level in Scoliotic Patients Operated with Novel Nickel-Titanium Superelastic Spinal Implant. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20 May 2010). 2010, 22-23.
Yeung K.W.K., Wu S.L., Tu H., Chu P.K., Liu X.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Suppression of Surface Nickel Concentration of Nickel Titanium Shape Memory Alloys by Plasma Surface Treatment., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 112-113.


Researcher : Cheung WY

List of Research Outputs

Chang K.C., Samartzis D., Fuego S.M., Dhatt S.S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Cheung K.M.C., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Cheung K.M.C., Samartzis D., Natarajan D., Li J., Cheung W.Y., Lenke L.G. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in scoliosis correction with alternate level pedicle screw fixation, Journal of Bone and Joint Surgery. 2010, 92: 169-176.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation., 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.. 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, J Bone Joint Surg (Am). 2010, 92: 169-76.
Cheung W.Y., Lenke L.G. and Luk K.D.K., Best Spine Paper Award. Prediction of scoliosis correction with thoracic segmental pedicle screw constructs using fulcrum bending radiograph., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., In-vivo demonstration of spontaneous vertebral derotation in thoracic adolescent idiopathic scoliosis with fulcrum bending radiograph, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Cheung W.Y., Lenke L.G. and Luk K.D.K., Prediction of scoliosis correction with thoracic segmental pedicle screw constructs using fulcrum bending radiographs, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Spine Chapter's Most Promising Spine Paper Award. Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Leung Y., Natarajan D., Samartzis D., Borkhuu B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis (AIS) using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Luk K.D.K., Vidyadhara S., Lu D.S., Wong Y.W., Cheung W.Y. and Cheung K.M.C., Coupling between sagittal and frontal plane deformity correction in idiopathic thoracic scoliosis and its relationship with post-operative sagittal alignment, Spine. 2010, 35(11): 1158-1164.
Wong Y.W., Cheung K.M.C., Cheung W.Y. and Luk K.D.K., The importance of spinal cord monitoring before and after turning the patients prone for operations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Chiu PKY

Project Title:The effect of bisphosphonate on established osteolytic lesions--a study of different approach and dosage in rabbit hip replacement model
Investigator(s):Chiu PKY, Tang WM, Zhu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Abstract:
To study the effect of different approach and dosage of bisphosphonate on bone loss in previous established rabbit osteolysis model after hip joint replacement.


Project Title:Role of Osteocyte in Sclerotic Subchondral Bone - A New Therapeutic Target for Osteoarthritis?
Investigator(s):Chiu PKY, Lu WW, Yan CH, Wen C
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:03/2010
Abstract:
Key issues Osteoarthritis (OA) is the most common form of arthritic disease, and it is a major cause of disability and impaired quality of life among the elderly. With a rapidly aging population in Hong Kong, OA causes a heavy socioeconomic burden. The Queen Marry Hospital, the investigators’ clinic, provides treatment for thousands of patients with end-stage hip or knee OA through arthroplasty, with a direct cost of 40,000 HKD/case/year [1-3]. Various methods, including matrix-associated autologous chondrocyte implantation (MACI), tissue engineering approach, stem cell therapy etc., have been studied in an attempt to slow down OA progression and thus reduce the demand on arthroplasty [4-7]. Yet those attempts have not demonstrated any significant clinical outcome because the pathophysiology of OA progression is not fully understood. OA is characterized by erosion of the articular cartilge, sclerosis of the subchondral bone and formation of osteophytes [8, 9]. It is conceptualized that the cross-talk between the subchondral bone and the articular cartilage is crucial for the initiation and progression of OA. Studies of human and animal OA models have demonstrated that sclerotic changes in bone are detected in the early stage of OA as “bone marrow lesions (BML)”and might even precede cartilage degeneration and loss [10-17]. Furthermore, the indices of bone resorption and formation are also found to be increased in OA patients [18, 19]. Yet the BMLs are sclerotic, and not well mineralized [11]. The enhanced bone remodeling and abnormal bone metabolism are associated with abnormal behavior of osteoarthritic osteoblasts [20]. Evidences also show that normal articular cartilage cocultured with osteoarthritic bone-derived cells lead to release of proteoglycans from the cartilage matrix. Whereas no such effect was observed when coculturing articular cartilages with normal bone-derived cells [21]. Thus, a primary osteoblastic defect has been proposed by some research groups to explain the subchondral bone disturbance and the breakdown of articular cartilage in the progression of OA. However, this postulation is challenged by the fact that osteoblasts have no direct physical contact with chondrocytes, and the evidence for the transmission of biochemical signals through the subchondral plate to have an effect on the overlying cartilage is lacking. Considering the difficulties in quantification of osteocytes number, their morphology and their alignment in three dimensions using the routine light and confocal microscopy, we recently identified nano-computed tomography (nano-CT) as a potential technique to overcome the limitations with the traditional microscopy. Our preliminary results from the nano-CT study have shown that the number of osteocyte lacunae increased but the individual lacunae were small in size with loss of alignment in the osteoarthritic bone. It was also noted that the osteoarthritic subchondral plate and articular cartilage were undermineralized, and the articular cartilage in higher Outbridge-grade OA lesions possessed lower mechanical stiffness. It is known that osteocytes play a central role in the regulation of bone remodeling and osteoblastic function by their production of sclerostin. In addition, osteocyte lacunae in subchondral plate directly contact with chondrocyte lacunae in calcified cartilage. Thus, we hypothesize that abnormal osteocytes are associated with the disturbance of subchondral bone metabolism, and mechanical failure of the overlying cartilage. Objectives This study is designed to (1) evaluate 3-dimensional osteocyte lacunae using nano-CT, (2) evaluate osteocyte-producing sclerostin expression, and (3) explore the potential relations of osteocyte morphology and function with bone miernal density distribution (BMDD), an indicator of bone metabolism, in osteoarthritic femoral heads taken from patients who undergo arthroplasty.


List of Research Outputs

Chiu P.K.Y., 1. Instructional Course Lecture: Revision total hip arthroplasty - hemispherical cementless acetabular component 2. Guest lecture: Factors affecting range of motion after total knee arthroplasty, Eleventh National Congress of Orthopaedic Surgery and the Fourth International Congress of Chinese Orthopaedic Association, organied by Chinese Medical Association and Chinese Orthopaedic Association, November 19-22, 2009 at Xiamen, China. 2009.
Chiu P.K.Y., 1. Revision total hip arthroplasty - the acetabular side 2. Management of bone defects in total knee arthroplasty, Henan Provincial Symposium on New Technologies in Total Joint Replacements, organized by Joint Surgery Section, Henan Orthopaedic Association, January 30-31, 2010 at Zhengzhou, Henan, China. 2010.
Chiu P.K.Y., 1. Selection of total knee prostheses in Chinese patients 2. Advances in revision total knee arthroplasty, The 2nd International Symposium on Orthopaedic Translational Research and Technical Advance, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, June 26-28, 2010 at Shanghai, China. 2010.
Chiu P.K.Y., Ng F.Y., Lee O.B., Tsang W.L., Wong H.L., Leung K.H., Wu W.C., Tang W.M., Chan W.S. and Lau W.L., A prospective, randomized, inter-regional, multi-centre study on gender solutions knee prostheses in Chinese females, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.
Chiu P.K.Y., Partial knee replacement - Does it work? Will it play more role?, Second National Advanced Seminar on total knee arthroplasty, organized by Joint Section of the Chinese Orthopaedic Association and Capitol Medical University Affiliated Chaoyang Hospital, June 12-13, 2010 at Beijing, China. 2010.
Chiu P.K.Y., Cheng H.C., Yau W.P., Tang W.M. and Ho H.S., Reading Radiographs after Total Knee Arthroplasty, Hong Kong Journal of Orthopaedic Surgery . 2010, 14 (1): 25 - 39.
Chiu P.K.Y., Revision total knee arthroplasty, 2009 Henan Provincial Annual Meeting of Chinese Medicine and Combined Chinese-Western Medicine in Orthopaedics & Traumatology, organized by Louyang Orthopaedic & Traumatology Hospital, November 26-27, 2009 at Louyang, Henan, China. 2009.
Chiu P.K.Y., The position of acetabular component in total hip arthroplasty for patients with ankylosing spondylitis, The third joint surgery seminar - Standards and developments in total hip arthroplasty surgical techniques, organized by First Affiliated Hospital of Sun Yat Sen University, September 19-20, 2009 at Guangzhou, China. 2009.
Chiu P.K.Y., Total hip replacement in ankylosing spondylitis, Eleventh scientific congress of Asia-Pacific Arthroplasty Society - September 4-6, 2009 at Xian, China. 2009.
Kuang G., Yau W.P., Lu W.W. and Chiu P.K.Y., Methods in enhancing osteointegration of soft tissue grafts within the bone tunnels in anterior cruciate ligament reconstruction, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S125.
Kuang G.M., Yau W.P., Lu W.W. and Chiu P.K.Y., Methods in Enhancing Osteointegration of Soft Tissue Grafts within the Bone Tunnels in Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Kuong E., Ng F.Y., Yan C.H., Chiu P.K.Y. and Fang C., Antibiotic Prophylaxis After Total Joing Replacements, Hong Kong Medical Journal. 2009, 15: 458-62.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Li Z., Lu W.W., Deng L., Chiu P.K.Y., Fang D., Lam R.W.M., Leong J.C.Y. and Luk K.D.K., The morphology and lattice structure of bone crystal after strontium treatment in goats, Journal Of Bone And Mineral Research. 2010, 28: 25-34.
Ma W., Ng F.Y., Chiu P.K.Y., Li Z. and Lu W.W., The effects of acetabular component position and hip ball diameter on posterior stability after total hip replacement, Chinese Journal Of Joint Surgery. 2009, 3: 624-629.
Ng F.Y., Chiu P.K.Y. and Yan C.H., Anterior Dislocation After Total Hip Replacement - Effects Of Pelvic Rotation And Hip Ball Size, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Ng F.Y., Jiang J.T., Chiu P.K.Y., Yan C.H. and Choi C.L., Anterior dislocation after total hip replacement - effects of pelvic rotation and hip ball size , The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S56.
Ng F.Y., Chiu P.K.Y., Yan C.H. and Ng K.F., Pain control after total knee replacement - comparison of local anaesthetics via continuous femoral catheter with patient-controlled analgesia, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.
Ng F.Y., Chiu P.K.Y. and Yan C.H., Posterior Dislocation After Total Hip Replacement - Effects Of Position Of Acetabular Shell And Hip Ball Size, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., Posterior dislocation after total hip replacement - the effect of position of acetabular shell and hip ball size, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S33.
Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., 全髋關節置換術後的前脫位- 骨盆旋轉和股骨頭大小的影響, The Eleventh National Congress Of Orthopaedic Surgery And The Fourth International Congress Of Chinese Orthopaedic Association. 2009, 64-65.
Ng J.K.F., Lawmin J.C., Tsang S.F., Tang W.M. and Chiu P.K.Y., The value of a single preoperative PFA-100® measurement in assessing the risk of bleeding in patients taking cyclooxygenase inhibitors and undergoing total knee replacement, British Journal of Anaesthesia. 2009, 102: 779-84.
Ni G., Lin J.H., Chiu P.K.Y., Li Z. and Lu W.W., Effect of strontium-containing hydroxyapatite bone cement on bone remodeling following hip replacement, Journal Of Material Science,. Materials In Medicine. 2010, 21: 377-384.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.
Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented Revision Total Hip Arthroplasty Using Impaction Bone Grafting And Elite Plus Stem: A Retrospective Review Of 15 Cases, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented revision total hip arthroplasty using impaction bone grafting and Elite plus stem: A retrospective review of 15 cases, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S58.
Yan C.H., Chiu P.K.Y. and Ng F.Y., QMH Experience in Primary TKR for Primary Knee Osteoarthritis 2000 - 2009, 7th Hong Kong International Orthopaedic Forum. 2010.
Yan C.H., Chiu P.K.Y. and Ng F.Y., 36毫米股骨頭在國人全髋置換中之效果, The Eleventh National Congress Orthopaedic Surgery And The Fourth International Congress Of Chinese Orthopaedic Association. 2009, 57.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The Radiographic Prevalence Of Knee Osteoarthritis In Local Chinese Subjects: A Retrospective Review Of 102 Angiogram Images, 29th Annual Congress Of The Hong Kong Orthopaedics Association. 2009.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The radiographic prevalence of knee osteoarthritis in local Chinese subjects: a retrospective review of 102 angiogram images, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S56.
Yan C.H. and Chiu P.K.Y., Total Hip Arthroplasty For Failed Internal Fixation Of Inter-trochanteric Fractures, 11th Asia-pacific Arthroplasty Society Annual Scientific Meeting. 2009.
Yan C.H., Chiu P.K.Y. and Ng F.Y., Total Hip Arthroplasty with 36mm Articulation in Chinese Patients, Annual Congress of Hong Kong Orthopaedic Association. 2009.
Yau W.P., Chiu P.K.Y. and Yan C.H., Cutting errors in Total Knee Replacement, 10th Annual Meeting of Computer Assisted Orthopaedic Surgery – International. 2010.
Yau W.P. and Chiu P.K.Y., Ligament balance for varus knee in computer navigation total knee replacement, 10th Annual Meeting of Computer Assisted Orthopaedic Surgery – International. 2010.
Zhao Z.Y., Chiu P.K.Y., Zhang S.D., Ma W.H., Yau W.P., Tang W.M., Wang S.J. and Wang W.G., A study on the effects of tibial cutting jig fixation on varus-valgus and anteroposterior slope of the tibial osteotomy, 胫骨平台切朴的因定方法对胫骨平台截骨在内外翻和前后倾方面影响的研究, Chinese Journal of Joint Surgery . 中国关节外科杂志, 2009, 3: 750 - 754.


Researcher : Chow SP

Project Title:Combined Congress of APFSSH, ISSH & ISRM Problems in Treatment of Phalangeal Fractures
Investigator(s):Chow SP
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:08/2000
Abstract:
N/A


List of Research Outputs

Cheung K.M.C., Yeung K.W.K., Lu W.W., Poon T.L. and Chow S.P., Fixation device, US Regular patent no. 7,695,471, granted on April 13, 2010, 2010.
Chow S.P. and Leung F.K.L., Chow SP, Leung F (2010): Radial and Ulnar Shaft fractures in Rockwood and Green’s Fractures in adults, Lippincott Williams & Wilkins; 7th edition: 881-904, USA, Lippincott Williams & Wilkins, 2010.
Ip W.Y., Chow S.P., Fung B.K.K. and Cheung J.P.Y., Occupational Repetitive Strain Injuries In Hong Kong, Hong Kong Journal Of Orthopaedic Surgery. Hong Kong, 2009, 13: 128.
Ip W.Y., Fung B.K.K., Chow S.P. and Wong S.S.Y., Treatment Of Mycobacterium Marinum Infection Of The Hand And Wrist In 166 Patients, The Hong Kong Journal Of Orthopaedic Surgery. Hong Kong, 2009, 65.


Researcher : Chow W

List of Research Outputs

To M.K.T., Chow W. and Umrani S., Early radiological results of the interlocking triple innominate osteotomy for hip dysplasia in adolescents, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
To M.K.T., Chow W. and Eid M., Hodgson Award, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
To M.K.T., Chow W., Li Y.H. and Eid M., Long-term efficacy of orthotic devices in children with flexible pes planus, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
To M.K.T., Chow W., Choi K.Y., Cheung H.M. and Liu K.L., Supracondylar fracture of humerus in paediatric and adolescent patients of Hong Kong, In: Michael To, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
To M.K.T., Chow W. and Tong S., Tong S, Chow W, To M. Ultrasound assessment and screening of developmental dysplasia of hips in Hong Kong, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Cui J

List of Research Outputs

Cui J., Wen C., Hu Y. and Luk K.D.K., Precise Diagnosis of Cervical Spondylotic Myelopathy based on Quantitative Fiber Tractography, The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Wen C., Cui J., Hu Y. and Luk K.D.K., Loss of Entropy in Fractional Anisotropy Mapping of Spinal Cord in Patients of Cervical Spondylotic Myelopathy., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010. . 2010.


Researcher : Fang D

List of Research Outputs

Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Li Z., Lu W.W., Deng L., Chiu P.K.Y., Fang D., Lam R.W.M., Leong J.C.Y. and Luk K.D.K., The morphology and lattice structure of bone crystal after strontium treatment in goats, Journal Of Bone And Mineral Research. 2010, 28: 25-34.


Researcher : Fok AWM

List of Research Outputs

Fok A.W.M. and Yau W.P., Isolated Bundle Tears of Anterior Cruciate Ligament: Incidence, Correlation with Tests and Injury to Surgery Duration, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Fok A.W.M. and Yau W.P., Isolated Bundle Tears of Anterior Cruciate Ligament: Incidence, Correlation with Tests and Injury to Surgery Duration, The Forth International Congress of Chinese Orthopaedic Association. 2009.
Fok A.W.M., Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The prevalence, factors, and clinical profile associated with tarlov cysts in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Fong MK

List of Research Outputs

Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.


Researcher : Fuego SM

List of Research Outputs

Chang K.C., Samartzis D., Fuego S.M., Dhatt S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Fung BKK

List of Research Outputs

Ip W.Y., Fung B.K.K. and Lee Y.E., Mycobacterium Chelonae Hand Infection:a Review Of Hand Sugery, Edmond Ted U. 2009, 14 (1): 7-13.
Ip W.Y., Chow S.P., Fung B.K.K. and Cheung J.P.Y., Occupational Repetitive Strain Injuries In Hong Kong, Hong Kong Journal Of Orthopaedic Surgery. Hong Kong, 2009, 13: 128.
Ip W.Y., Fung B.K.K. and Cheung J.P., Review Article: Mycobacterium Marinum Infection Of The Hand And Wrist, Journal Of Orthop Surgery (Hong Kong). Hong Kong, Hong Kong Medical Journal, 2010, 18: 98-103.
Ip W.Y., Fung B.K.K., Cheung J.P. and Wong S.S., Review Article: Mycobacterium Marinum Infection Of The Hand And Wrist, Journal Orthop Surgery (hong Kong). Hong Kong, 2010, 18(1): 98-103.
Ip W.Y., Fung B.K.K., Chow S.P. and Wong S.S.Y., Treatment Of Mycobacterium Marinum Infection Of The Hand And Wrist In 166 Patients, The Hong Kong Journal Of Orthopaedic Surgery. Hong Kong, 2009, 65.


Researcher : Grozman SAM

List of Research Outputs

Saw L.B., Cheung K.M.C., Grozman S.A.M. and Luk K.D.K., A practical classification of the distal radius and ulna radiograph for assessing skeletal maturity, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.


Researcher : Guo XX

List of Research Outputs

Chu C.L., Guo X.X., Sheng X.B., Dong Y.S., Lin P.H., Yeung K.W.K. and Chu P.K., Microstructure, Nickel Suppression, and Mechanical Characteristics of Electropolished and Photoelectrocatalytically Oxidized Biomedical Nickel Titanium Shape Memory Alloy, Acta Biomaterialia. 2009, 5(6): 2238-2245.
Peng S.L., Liu X.W., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., Effect of different timing of strontium treatment on bone remodeling in ovariectomized rats, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Hu L

List of Research Outputs

Hu L., Mouraux A., Hu Y. and Iannetti G.D., A novel approach for enhancing the signal-to-noise ratio and detecting automatically event-related potentials (ERPs) in single trials., Neuroimage. 2010, 50: 99-111.


Researcher : Hu Y

Project Title:Novel method for fast extraction and auto-recognition of somatosensory evoked potentials
Investigator(s):Hu Y, Luk KDK, Chan SC, Chang C
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2007
Abstract:
(1) Purpose of investigation: Intraoperative spinal cord monitoring can significantly minimize the risks of iatrogenic injury to spinal cord during spinal surgery, neurosurgery and aortic surgery. Somatosensory evoked potential (SEP) is the most popular technique currently being used in the operating room. The ensemble averaging method (EA) is the only practical method to extract SEP, but it will considerably lengthen the surgical time and hinder the surgical procedures, and more importantly, it cannot provide the spine surgeon with a timely warning of the eminent danger of cord injury. This motivates us to study and develop a new and improved method for fast extraction and automatic recognition of SEP.(2) Key issues and major problems: The main problem in SEP monitoring is its extremely low signal to noise ratio (SNR) and variability in its signal waveform. The EA method is used to extract SEP from background noise, but it is time-consuming. Moreover, the noisy waveform makes the auto-recognition of abnormal changes in SEP a difficult task. Sometimes, latency/amplitude measurement based on time-domain SEP waveform is highly variable.(3) Objectives and Possible outcomes: This project has 3 main objectives: i) To study and develop a signal processing technique for improving the SNR of SEP signals. We shall study and apply a multi-adaptive filter in combination with a novel data-reusing non-linear method based on radial basis function neural network (RBFNN) for improving the SNR of SEP signals. (4) ii) To study and develop a fast extraction method for separating the SEP signal from other interference based on multi-channel blind source separation. The use of a multi-adaptive filter can improve the SNR of SEP signals, but it may not be sufficient to obtain a reliable SEP signal by a single trial. The multi-channel blind source separation (BSS) will be used to enhance the performance of the algorithm so that a more reliable and reproducible SEP can be obtained from every single trial. Moreover, the time-frequency feature of SEP will be investigated to establish a time-frequency model for auto-classification and identification of SEP waveform. (5) iii) To investigate and develop an auto-recognition method based on time-frequency analysis of SEP. The developed fast extraction and auto-recognition methods will be applied to clinical and animal experiments and the specificity and sensitivity can be then evaluated. (6) Long-term impact, relevance, significance and value: The findings of this research will contribute to the further development of fast and reliable techniques for SEP monitoring. The ultimate goal is to provide safer and more reliable monitoring, which is more easy to carry out during surgery. In addition, the results of this research would benefit the further development in SEP diagnosis of neuromuscular disorders. On the engineering perspective, the signal processing algorithms and results so developed will have considerable impact on the theory and practice of multi-channel signal processing, especially in neural engineering and electroencephalograph extraction. Last but not least, the future application of this study can be developed into a product with economical benefit for Hong Kong.


Project Title:Studying Effectiveness and Compliance of Neck Support for Car Repairing Workers
Investigator(s):Hu Y, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:The OSH Enhancement Scheme for SMEs
Start Date:01/2007
Abstract:
To study the effectiveness and compliance of neck support to prevent car repairing workers from chronic occupational neck injury, so as to improve the design of neck support to be a popular acceptable product.


Project Title:Motor neurological functional reconstruction and rehabilitation of lower limb nervous system in paraplegics
Investigator(s):Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:Travel Grants for NSFC/RGC JRS
Start Date:12/2007
Abstract:
Travel grants for NSFC/RGC JRS.


Project Title:Biomedical and electrophysiological guidance for low back pain rehabilitation and prevention
Investigator(s):Hu Y, Luk KDK, Kwok HY
Department:Orthopaedics and Traumatology
Source(s) of Funding:S.K. Yee Medical Foundation - General Award
Start Date:01/2008
Abstract:
To establish a practical guidance for identifying those LBP who are likely to benefit from non surgical treatments; to develop individualized biomechanical and electrophysiological guidance rehabilitation service which reveals underlying functioning status of back muscles under treatment, and provide guidance for patients to achieve optimal back muscle activity which provides better rehabilitation outcomes.


Project Title:Time-frequency componets analysis of evoked potentials for determination of function deficits localization in spinal cord
Investigator(s):Hu Y, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:04/2008
Completion Date:03/2010
Abstract:
The objectives of this research are 1) to develop the signal processing technique based on time-frequency analysis to extract various components in time-frequency distribution of somatosensory evoked potential (SEP) signals; 2) to investigate the correlation between the changes of different signal components with pathology causes (ischemia or mechanical). SEP has been widely used in intraoperative spinal cord monitoring. However, from the surgeon's perspective, intraoperative electrophysiological monitoring should also aid identification of the neurological cause of abnormality and location of the damaged cord tracts, so as to indicate clear correlation between changes of SEP and action of the surgeon. The problem faced is how to manipulate the SEP waveform changes to provide meaningful indications of pathomechanism cause and location. Therefore, the key issue here is to disclose the injury-associated time-frequency distribution of SEP, identify the component reflecting different cord tracts, and develop novel signal processing techniques and decomposition algorithms that can extract precise information about the nature of spinal cord injury. The expected outcome of this project is a precise intraoperative spinal cord monitoring technique, allowing the accurate identification of the pathological cause and location of the spinal cord injury.


Project Title:Hardware oriented processor for evoked potential fast extraction and auto-detection
Investigator(s):Hu Y, Luk KDK, Cheung PYS, Wong YW, Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Innovation and Technology Support Programme (Tier 3)
Start Date:12/2008
Completion Date:05/2010
Abstract:
1. Real-time signal processor for fast extraction of evoked potential: we will develop hardware oriented signal noise ratio enhancer to extract evoked potential from noisy background. This technology will be implement in FPGA based circuit module, which can be adopted by different electrophysiology equipments to improve recording speed and quality. 2. Automatic evoked potential detector for intraoperative monitoring: we will develop a real-time detector based on hardware environment to classify normal and abnormal signals in reflecting spinal cord functional change.


Project Title:Phase-based spectral analysis of surface array EMG for lumbar neuromuscular functional assessment
Investigator(s):Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:Travel Grants for NSFC/RGC JRS
Start Date:12/2008
Abstract:
Travel grants for NSFC/RGC JRS


Project Title:Dynamic surface electromyography topography for objective assessment of low back pain
Investigator(s):Hu Y, Chen YH, Luk KDK, Wang WP
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2009
Abstract:
1. To develop techniques to quantify the sEMG topography patterns and time-varing features of dynamic sEMG; 2.To investigate the dynamic sEMG topography patterns associated with LBP and prove their significant difference from the patterns in healthy subjects; 3. To investigate the feature changes of dynamic sEMG topography patterns in subjects with LBP between pain situation and pain release situation, therefore to demonstrate the ability of sEMG to assess the LBP rehabilitation progress; 4. To develop a demonstration system of dynamic sEMG topography analysis.


Project Title:Development of a biomedical signal acquisition and processing experiment and teaching kit
Investigator(s):Hu Y, Chang C
Department:Orthopaedics and Traumatology
Source(s) of Funding:Leung Kau Kui Research and Teaching Endowment Fund - Teaching Grants
Start Date:04/2009
Abstract:
The objectives of this study are 1: to develop a demonstration and teaching kit for undergraduation and post-graduation courses on Biomedical engineering, biosignal processing and system, signal processing, and clinical electrophysiology relevant courses, 2:To improve the teaching efficiency and quality of biomedical signal acquisition and processing. The key issues and problems: it is always a difficult topic in the detection, understanding and identification of biomedical signals and signal processing. Up to now, the learning of signal processing and biomedical engineering is mainly by lectures and only can be described in theory express. On the other hand, the undergraduate students in medical engineering programme and research students in both faculty of medicine and Engineering usually feel very difficulty to understand the principle and application of various signal processing, especially some advanced signal processing algorithms for non-stationary biomedical signals (e.g. electromyogram and electroencephalogram).


Project Title:Using Multi-channel electroencephalography (EEG) to Reveal The Reality And Intensity Of Pathological Back Pain
Investigator(s):Hu Y, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:04/2009
Abstract:
The project objectives are: 1) establish multi-channel EEG method with somatosensory stimulation under pain and pain-free situation; 2) develop new signal analysis of SEP patterns, source tracking and connectivity for difference of EEG activities in back pain from non-pain; 3) identify and quantize special components related to painful sensation input.


Project Title:Hardware Oriented Processor for Evoked Potential Fast Extraction and Auto-detection
Investigator(s):Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:Innovation and Technology Fund Internship Programme
Start Date:09/2009
Abstract:
a) Real-time signal processor for fast extraction of evoked potential: we will develop hardware oriented signal noise ratio enhancer to extract evoked potential from noisy background. This technology will be implement in FPGA based circuit module, which can be adopted by different electrophysiology equipments to improve recording speed and quality. b) Automatic evoked potential detector for intraoperative monitoring: we will develop a real-time detector based on hardware environment to classify normal and abnormal signals in reflecting spinal cord functional change.


Project Title:Hardware Oriented Processor for Evoked Potential Fast Extraction and Auto-detection
Investigator(s):Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:Innovation and Technology Fund Internship Programme
Start Date:09/2009
Abstract:
a) Real-time signal processor for fast extraction of evoked potential: we will develop hardware oriented signal noise ratio enhancer to extract evoked potential from noisy background. This technology will be implement in FPGA based circuit module, which can be adopted by different electrophysiology equipments to improve recording speed and quality. b) Automatic evoked potential detector for intraoperative monitoring: we will develop a real-time detector based on hardware environment to classify normal and abnormal signals in reflecting spinal cord functional change.


Project Title:Skeletal muscular dynamic regulatory mechanisms during neurological functional reconstruction and rehabilitation of paraplegic walking
Investigator(s):Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:Travel Grants for NSFC/RGC JRS
Start Date:12/2009
Abstract:
Travel grants for NSFC/RGC JRS


Project Title:Implementation of individual brain coputer interface design for spinal cord injury patients
Investigator(s):Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:S.K. Yee Medical Foundation - General Award
Start Date:03/2010
Abstract:
n/a


Project Title:Maximum lumbar muscle activity estimation by multivariate functional data analysis of surface EMG in sudden-load release
Investigator(s):Hu Y, Zhang Z, Chen YH, Chan SC
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:03/2010
Abstract:
Maximum voluntary activation is defined as the level of neural drive to a muscle during maximum contraction. If the maximum voluntary activation could be feasibly measured in the lumbar muscles, it could be used to further our understanding of how back muscle physiology is altered in sufferers of chronic low back pain, to accurately assess the muscle strength, and to provide correct baseline for bio-feedback treatment. However, the measurement of lumbar muscle maximum activation is not an easy task as there can be errors caused by the level of sincerity or motivation of the subjects, or as is the case in some populations, such as back pain patients, due to fear of (re)injury or pain. This project proposes a new method to predict maximum voluntary activation by measuring involuntary lumbar muscular response based on surface electromyography (sEMG) under an experimental protocol of sudden load release. Since this quick involuntary muscle response during sudden load release is an indicator of a reflex rather than a voluntary response, it avoids possible bias introduced by subjectivity of people under test and provides a more objective measurement of muscle activity. In combination of regressive prediction by a series of sub-maximum muscle activity and in-volitional activation test by sudden load-release, a new method to estimate maximum activity in lumbar muscles will be developed in this study. The Objectives of this study are: 1) A system for sudden load release test will be designed to generate sudden load release at different weights, to collect surface electromyegraphy (sEMG) signals in a high precision, and to monitor other useful information. In addition, advanced sEMG signal processing and features extraction techniques will be developed or applied to provide low-SNR signals and accurate features. 2) a functional linear regression model to describe the relationship between maximum activities (as responses) and functional sEMG features (as predicators) will be developed. Some important scalar sEMG features and anthropometric data will be recruited as predicators in the model as well. Furthermore, we shall develop appropriate variable selection and model estimation methods to find significant variables (features) with good predictability for the maximum activities and try to establish a predication model.


Project Title:26th Annual Meeting of the European Section of the Cervical Spine Research Society The Entropy in Fractional Anisostropy Mapping of Healthy and Injured Spinal Cord of Human beings Ultrastructural imaging of Cervical Spinal Cord with Induced Compressive Injuries in a Rat Model
Investigator(s):Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:05/2010
Completion Date:05/2010
Abstract:
N/A


Project Title:Development of novel surface electromyegraphy dynamic topography analysis system for back pain assessment
Investigator(s):Hu Y, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:06/2010
Abstract:
Low back pain (LBP) is a complex symptom, making objective measurement and precise diagnosis difficult. Dynamic sEMG topography is capable of revealing and recording in vivo trunk muscular activity non-invasively. Our previous study had found the quantitative analysis of dynamic sEMG topography could enable the ability of LBP assessment and the indication of pain release. The objectives of this project are: 1. To design a flexible electrode-array and prepare a patent application 2. To develop techniques to quantify the sEMG topography patterns and time-varing features of dynamic sEMG, and to develop an autometic sEMG topography analysis program. 3. To develop a demonstration system capable of dynamic sEMG topography analysis and computer-user interaction interface.


List of Research Outputs

Arjunan S.P., Kumar D.K., Poon W.M., Rudolph H. and Hu Y., Variability in Surface Electromyogram During Gait Analysis of Low Back Pain Patients, Journal of Medical and Biological Engineering. 2009, 30(3): 133-138.
Cui J., Wen C., Hu Y. and Luk K.D.K., Precise Diagnosis of Cervical Spondylotic Myelopathy based on Quantitative Fiber Tractography, The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Hu L., Mouraux A., Hu Y. and Iannetti G.D., A novel approach for enhancing the signal-to-noise ratio and detecting automatically event-related potentials (ERPs) in single trials., Neuroimage. 2010, 50: 99-111.
Hu Y., Siu H.F., Mak N.F.J. and Luk K.D.K., Lumbar Muscle Electromyographic Dynamic Topography During Flexion-extension., J Electromyogr Kinesiol.. 2010, 20: 246-55.
Hu Y., Siu H.F., Mak J.N.F. and Luk K.D.K., 256. Lumbar muscle electromyographic dynamic topography during flexion-extension, Journal of Electromyography and Kinesiology. 2010, 20: 246-255.
Hu Y., Brain computer interface and its potential use in rehabilitation., Trauma Rehabilitation- a multidisciplinary approach.. Hong Kong, 2010.
Hu Y., Wong Y.L., Lu W.W. and Kawchuk G.N., Creation of an asymmetrical gradient of back muscle activity and spinal stiffness during asymmetrical hip extension., Clin Biomech. 2009, 24: 799-806.
Hu Y., Measurement and instrument technology in neuralengineering: the state-of-the-art. , 2010 International Conference on Measuring Technology and Mechatronic Automation(ICMTMA2010). Changsha, China, 2010.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Multiple Bioimaging Modalities Application for Chronic Compressive Spinal Cord of Rat. , the 54th Annual Meeting of the Orthopedic Research Society. New Orleans, Louisiana; 2010.
Hu Y., Multiple Bioimaging Modalities Application for Spinal Cord Chronic Compression in a Rat Model., Fourth International Congress of Chinese Orthopaedic Association (COA2009). Xiamen, China, 2009.
Hu Y., Siu H.F. and Luk K.D.K., Quantitative surface electromyography topography for low back pain assessment, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Hu Y., Liu H. and Luk K.D.K., Signal-to-Noise Ratio of Intraoperative Tibial Nerve Somatosensory-Evoked Potentials. , J Clin Neurophysiol.. 2010, 27: 30-3.
Hu Y., Liu H. and Luk K.D.K., Signal-to-noise ratio of intraoperative tibial nerve somatosensory-evoked potentials, Journal of Clinical Neurophysiology. 2010, 27(1): 30-33.
Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment. , The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK).. Aalborg Denmark., 2010.
Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment., The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK). 16-19 June, 2010, Aalborg Denmark. 2010.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Ultrastructural imaging of Cervical Spinal Cord with Induced Compressive Injuries in a Rat Model., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Li T.H., Luk K.D.K., Hu Y. and Wu E.X., Ultrastructural imaging of the cervical spinal cord , 2010.
Long H.O.U.-.Q.I.N.G., Wen C., Hu Y., Liu S.H.A.O.-.Y.U. and Li F.U.-.B.A.O., The Establishment Of The Experimental Chronic Compressive Myelopathy And The Mechanism Of Somatosensory-evoked Potential's Evaluation , 慢性压迫性脊髓症研究平台的建立及体感诱发电位功能评价的机制, Chinese Journal of Orthopedics. 中华骨科杂志, 2010.
Ming D., Bai Y., Liu X., Qi H., Cheng L., Wan B., Hu Y., Wong Y.W., Luk K.D.K. and Leong J.C.Y., A gait stability investigation into FES-assisted paraplegic walking based on the walker tipping index. , J Neural Eng. 2009, 6: 6600-7.
Ming D., Hu Y., Zhang G.J., Xue Z.J., Li L.Y., Liu X.Y., Bai Y.R., Cheng L.L., Qi H.Z., Wan B.K. and Luk K.D.K., Measurement of upper extremity joint moments in walker-assisted gait, IET Science, Measurement & Technology. 2009, 3(5): 343-353.
Ming D., Hu Y., Wong Y.W., Wan B.K., Luk K.D.K. and Leong J.C.Y., Risk-tendency graph (RTG): a new gait-analysis technique for monitoring FES-assisted paraplegic walking stability., Med Sci Monit. . 2009, 15: 105-12.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Ren Z.L., Zou Y.X., Zhang Z. and Hu Y., Fast extraction of somatosensory evoked potential using RLS adaptive filter algorithms, International Conference on Image and Signal Processing/International Conference on BioMedical Engineering and Informatics (CISP2009-BMEI2009). 2009.
Wen C., Cui J., Hu Y. and Luk K.D.K., Loss of Entropy in Fractional Anisotropy Mapping of Spinal Cord in Patients of Cervical Spondylotic Myelopathy., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010. . 2010.
Wen C., Hu Y., Li T.H., Lam K.O., Wu E.X. and Luk K.D.K., Multiple bio-imaging evaluations of spinal cord chronic compression in rat, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Zhang Y.Q., Cui H.Y., Zhang Z., Xie X.B. and Hu Y., Real-time somatosensory evoked potential monitoring using FPGA-based adaptive filter, The 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC 2009). 2009.
Zhang Z., Luk K.D.K. and Hu Y., Identification of detailed time-frequency components in somatosensory evoked potentials, IEEE Transactions on Neural Systems and Rehabilitation Engineering. 2010, 18: 245-254.
Zhang Z., Hung Y.S., Chan S.C., Xu W. and Hu Y., Modeling and identification of gene regulatory networks: A Granger causality approach, International Conference on Machine Learning and Cybernetics (ICMLC2010). 2010.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: Methods and comparisons. , J Electromyogr Kinesiol.. 2010, 20: 89-101.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: methods and comparisons, Journal of Electromyography and Kinesiology. 2010, 20: 89-101.
Zhu F., Maxwell J.P., Hu Y., Zhang Z...G., Lam W.K., Poolton J.M. and Masters R.S.W., EEG activity during the verbal-cognitive stage of motor skill acquisition.. Biological Psychology, 2010, 84(2): pp.221-227.
Zhu F., Maxwell J.P., Hu Y., Zhang Z., Lam W.K., Poolton J.M. and Masters R.S.W., EEG activity during the verbal-cognitive stage of motor skill acquisition. , Biological Psychology . Elsevier, 2010, 84: 221-227.


Researcher : Huang S

List of Research Outputs

Liao H., Huang S., Cheung K.M.C., Chan D., Luk K.D.K. and Zhou G., Functional characterization of miRNA125b, miRNA140 and miRNA199a in chondrogenetic differentiation of mesenchymal stem cells, Cell Adhesion & Communication.. 2009, In press.


Researcher : Ip WY

Project Title:Study on wrist problems
Investigator(s):Ip WY, Chow SP
Department:Orthopaedics and Traumatology
Source(s) of Funding:Other Funding Scheme
Start Date:01/1996
Abstract:
To study the clinical problems of chronic wrist pain. The individual specific clinical problems including sequalae of fracture distal radius, carpal instability, distal radio-ulnar joint problems, triangular fibrocartilage complex lesions and ulnar abatment syndromes would be studied in details. The objectives are to find the best treatment methods and to improve the functional results.


Project Title:The study of work related hand injuries in Hong Kong
Investigator(s):Ip WY, Chow SP
Department:Orthopaedics and Traumatology
Source(s) of Funding:Occupational Safety and Health Council - Research Grant Projects
Start Date:01/1998
Abstract:
To study the epidemiology of severe hand injuries which are work related in Hong Kong Island; to suggest more accurate prognostic factors and more reasonable disability assessment system of hand injury; to give suggestions in industrial safety; to improve management of severe hand injuries; to minimise the social cost of severe hand injuries.


Project Title:29th Annual Meeting of the Controlled Release Society Case Report Using Resorbable Polylactide Sponge for Controlled Release of Vancomycin in Methicillin Resistant Staph Aureus Osteomyelitis
Investigator(s):Ip WY
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:07/2002
Abstract:
N/A


Project Title:International Meeting on Upper Limb in Tetraplegia A Local Experience of Upper Limb Surgery for Tetraplegia in Hong Kong
Investigator(s):Ip WY
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:09/2007
Abstract:
N/A


Project Title:Investigation on advanced composite material with bioactivity and degradability for tendon regeneration
Investigator(s):Ip WY
Department:Orthopaedics and Traumatology
Source(s) of Funding:Small Project Funding
Start Date:01/2009
Abstract:
PURPOSE The current project aims to develop an innovative composite biomaterial that has desirable mechanical properties abd bioactivity. KEY ISSUES Polyurethane is a class of elastic poltmer with wide range of industrial and medical applications, and its use as a biomaterial has been emerging. Its elasticity is much higher than most medical polymeric materials, and the acidic product produced is much less than polylactides and polyglycolides. Similar toother polymers, however, the bioactivity of polyurethane is not sufficiently high. It was found that magnesium, tungsten, zinc and their alloys may be resorbed in vivo and act as temporary implants. Magnesium implants were also found to induce bone healing, and it is total non-toxic when used in a controlled amount. However, the stiffness will likely disallow the use of metallic magnesium as a biomaterial for soft tissue engineering when used alone. We propose that: 1) The innovative inclusion of magnesium wires into a polymeric polyurethane scaffold will: (a) reinforce the polymer; (b) reduce the acidity during the degradation process (acidity can cause tissue damage); (c) bioactivity of the composite will be increased when magnesium ions are released, as it was previously found that magnesium ions can promote bone healing. 2) The elasticity of polyurethane will be restored when the magnesium wires partially degrade in the body; providing an elastic environment similar to natural tendon, in turn stimulating the normal regeneration of tendon. Our experiments will determine whether the hypotheses above are valid.


Project Title:Flexible nerve guidance conduit for repairing peripheral nerve injury
Investigator(s):Ip WY, Yeung KWK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:01/2010
Abstract:
To investigate a flexible nerve guidance conduit fabricated by a newly developed biodegradable polymer accommodating for long peripheral nerve regeneration On the peripheral nerve injury (PNI), the common practices are coaptation for short lesions while suture with a nerve autograft for longer gaps. However, both of them are not perfect even the latter one was called ‘the golden method’. The coaptation may generate a certain degree of stress on PNI, which will suppress the regeneration of axon as well as the functional recovery of the nerve. ‘The golden method’ requires multiple surgeries for the extraction and the implantation of the nerve autograft. Also, the nerve donor site morbidity is a major concern due to functional loss. Furthermore, the supply of the nerve autograft is limited due to the dimensional suitability. Concerning all these limitations, an artificial nerve guidance conduit (NGC) has been developed over 20 years but the breakthrough is limited due to the limited knowledge of the interaction between the cells and scaffold. At present, the clinically used NGCs have a critical length of repair, 3cm , which is relatively short comparing to most of the nerve injuries. Thus, there is a need to develop a novel NGC solving the structural and functional deficits of existing nerve conduits. Currently, degradable biomaterials are mainly used as nerve guidance conduit (NGC) since it does not require the post-extraction operation. Also, the presence of non-degradable NGC may induce inflammation as it is a foreign material to our body. In the peripheral nerve repair strategies, NGC is used to direct and protect the regenerated nerve fibers from proximal stump to the proper distal stump so as to the appropriate targets. The popular biomaterials being investigated include poly (lactic acid) [1, 2], poly (lactic-co-glycolic acid) [3, 4, 5, 6], poly (ε-caprolactone) [7, 8], polyhydroxybutarate [9] and poly (glycolic acid) [10, 11]. Neither natural nor synthetic polymers can be a perfect NGC. The former one will lose its mechanical properties during the early degradation, whereas the latter one is usually hydrophobic, thereby influencing cell adhesion [12]. To tackle the problems of these polymers, a new biodegradable cross-linked polymer, crosslinked urethane-doped polyester elastomer (CUPE), has been invented [13]. To fabricate an ideal NGC, the mechanical and biological properties of materials are critical. Ideally, the materials should be biodegradable, biocompatible, mechanically robust, good permeability and excellent flexibility. Lastly, the degradation rate of NGC should be similar to the rate of axonal regeneration such that the presence of NGC will not induce any compression on the regenerated axons. During the repairing process of PNI, cells require nutrients and oxygen to maintain their growth. Therefore, the permeability of NGC is a concern. More importantly, the initial stage of regeneration may also be affected by permeability of NGC. The permeability of NGC influences the formation of fibrin matrix. With a stable fibrin matrix, the neural cell migration and regeneration can be improved. [15, 16] For the long lesion repairing, the flexibility of NGC is counted as a factor since the NGC may also need to be implanted across movable joints. Unfortunately, the flexible materials are often weak such that the flexible NGC may be easily broken or torn. Thus, the balance on the mechanical properties of the materials should be done carefully in term of different dimensions and internal frameworks. [17] The degradation of biodegradable NGC may also contribute to the swelling and compression to the regenerated axons due to the formation of degradation product [17]. If the degradation is too fast, the patient will be suffered from swelling. On the other hand, the slow degradation of NGC will lead to the compression on the regenerated axons or even a chronic foreign body reaction [17]. Therefore, the degradation rate of NGC has to be adjusted. The newly developed CUPEs from our oversea co-investigator, Dr. Yang’s group [13] can absolutely fulfill all the requirements as NGCs. Generally, the polymers are made of citric acid, 1,8-octanediol and 1,6-hexamethyl diisocyanate (HDI). Within the structure, there are many cross-linkages, which connect the polymer chains and provide the ability on resisting the mechanical distortion. When force is applied, the cross-linkages will be distored and the internal force will be stored. Once the force is removed, the internal force is released such that the structure is restored. Therefore, CUPEs have an excellent elasticity. This specific property favors for long peripheral nerve lesion, as NGC can provide sufficient mechanical flexibility when the connection involves joints in between. Apart from the special restoration property, the mechanical properties and the biodegradability of the polymers can be modulated by controlling the synthesis conditions as well as the monomers used. Therefore, the properties of these polymers can be adjusted as similar as the natural nerves. [13, 14] More importantly, CUPEs have been tested for it biocompatibility with NIH 3T3 fibroblast and human aortic smooth muscle cells (HASMCs). The inflammatory response for CUPEs is weaker than the PLLA implants. Therefore, it implies the CUPEs are more suitable as an implantation than PLLA. [13] Since the polymers have not yet investigated as materials for NGCs, there is a need to study if it is suitable for nerve regeneration. If succeeded, we could establish this new biomaterial, which is easy to fabricate and has controllable properties. The reason to apply for seed funding is to investigate the feasibility of this new polymer, CUPE, as NGCs. Firstly, cell culture with the use of hMSC derived Schwann cells will be carried out to evaluate the biocompatibility of this cross-linked polymer as well as the growth pattern of Schwann cells. Secondly, the polymer will be fabricated into a designed shape for in vivo study. After obtaining the preliminary results, we will submit a more elaborate application to external funding bodies such as RGC GRF for comprehensive mechanical and biological studies to expand this new NGC for long peripheral nerve lesion in clinical use. The proof of concept must be done and help build up the foundation for substantial grant application in future.


List of Research Outputs

Ip W.Y., Bone Regeneration with resorbable Poly-lactide membrance & sponge in a unstable fracture model in rabbit radii & Clinical application, Post conjoint examination academic conference in Orthopaedic Surgery. 2009.
Ip W.Y., Bone Regeneration with resorbable poly-lactide membrance & spongy in an unstable fracture model in rabit radii & clincal application, Universities of Texas Conjoint conference in Biomedical Engineering . 2009.
Ip W.Y., Diabetic Peripheral Artery Disease, 6th Asia Pacific Diabetic Limb Problems Meeting. 2009.
Ip W.Y., Diabetic peripheral artery disease, 6th Asia Pacific Diabetic Limb Problems Meeting & 5th Beijing International Forum on Diabetic Foot and Related Disease . 2009.
Ip W.Y., Finger Joint Arthroplasty and the New Design Concepts , 8th Congress of Asia Pacific Federation for Surgery of the Hand. 2009.
Ip W.Y., Finger Joint Arthroplasty and the New Design Concepts , Post conjoint examination academic conference. 2009.
Ip W.Y. and Yuen C.K., Irrelevance Of Simple Immersion Models On The Degradation Behavior Of Degradable Metallic Implants, The Society For Biomaterials 201 Annual Meeting. USA, 2010.
Ip W.Y., Models Of Multi-disciplinary Collaborative Platform For Early Intervention Of Patients With Ischaemic Diabetic Limb, 4th Scientfifc Congress Of Vascular And Intervenational Radiology Foundation . 2009.
Ip W.Y., Fung B.K.K. and Lee Y.E., Mycobacterium Chelonae Hand Infection:a Review Of Hand Sugery, Edmond Ted U. 2009, 14 (1): 7-13.
Ip W.Y. and Lee Y.E., Necrotizing Fasciitis Of The Extremity Caused By Haemophilus Influenzae Serotype B In A Healthy Adult, Clin Orthop Relat Res. 2010, 468(5): 1436-9.
Ip W.Y., New Endoscopic Assisted Cubutal Tunnel & Carpal Tunnel Release , Post conjoint examination academic conference. 2009.
Ip W.Y., Chow S.P., Fung B.K.K. and Cheung J.P.Y., Occupational Repetitive Strain Injuries In Hong Kong, Hong Kong Journal Of Orthopaedic Surgery. Hong Kong, 2009, 13: 128.
Ip W.Y., Chen P.P., Lam M.K., Mok V., Tsoi T.H., Wong C.P. and Wong H.S., Recommendation For The Management Of Crps, Medical Progress. Hong Kong, 2009, 421-427.
Ip W.Y., Fung B.K.K. and Cheung J.P., Review Article: Mycobacterium Marinum Infection Of The Hand And Wrist, Journal Of Orthop Surgery (Hong Kong). Hong Kong, Hong Kong Medical Journal, 2010, 18: 98-103.
Ip W.Y., Fung B.K.K., Cheung J.P. and Wong S.S., Review Article: Mycobacterium Marinum Infection Of The Hand And Wrist, Journal Orthop Surgery (hong Kong). Hong Kong, 2010, 18(1): 98-103.
Ip W.Y., Chen P.P., Lam M.K., Mok V., Tsoi T.K., Wong C.P. and Wong H.S., Review Of Fibromyalgia , Challenge In Neuropathic Pain. 2009, 18.
Ip W.Y. and Yuen C.K., Theoretical Risk Assessment Of Magnesium Alloys As Degradable Biomedical Implants, Acta Biomaterials. Europe, 2010, 6(5): 1808-12.
Ip W.Y., Fung B.K.K., Chow S.P. and Wong S.S.Y., Treatment Of Mycobacterium Marinum Infection Of The Hand And Wrist In 166 Patients, The Hong Kong Journal Of Orthopaedic Surgery. Hong Kong, 2009, 65.
Ip W.Y., Wound Coverage And Nerve Decompression Surgery To Prevent Major Amputation In Diabetic Foot Disease, 6th Asia Pacific Diabetic Limb Problems Meeting. 2009.
Ip W.Y., sub-editor, Hand Surgery. 2010.
Zhou W., Wang M., Cheung W.L. and Ip W.Y., Selective Laser Sintering of Poly(L-Lactide)/Carbonated Hydroxyapatite Nanocomposite Porous Scaffolds for Bone Tissue Engineering, In: Daniel Eberli , Tissue Engineering. Vienna, Austria, IN-TECH, 2010, 179-204.


Researcher : Kuang G

List of Research Outputs

Kuang G., Yau W.P., Lu W.W. and Chiu P.K.Y., Methods in enhancing osteointegration of soft tissue grafts within the bone tunnels in anterior cruciate ligament reconstruction, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S125.


Researcher : Kwok HY

List of Research Outputs

Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment. , The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK).. Aalborg Denmark., 2010.
Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment., The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK). 16-19 June, 2010, Aalborg Denmark. 2010.


Researcher : Lai DWT

List of Research Outputs

Yeung K.W.K., Lai D.W.T., Lai D.W.T., Lai D.W.T., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Wu S.L., Tu H., Chu P.K. and Chung C.Y., Biomechanical Analysis of Plasma Treated NiTi Materials for Spinal Deformity Correction., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May 2010). 2010, 24-25.


Researcher : Lam KO

List of Research Outputs

Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A Biodegradable Polymer-Based Coating To Control The Performance Of Magnesium Alloy Orthopaedic Implants, Biomaterials. 2010, 31(8): 2084-209.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., A New PCL Based Porous Membrane to Control the Degradation of Biodegradable Metallic Materials., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug–3Sep 2009. Poster presentation.. 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., Reduction of the Corrosion Rate of Biodegradable Metallic Materials by Al2O3 PIII surface Treatment., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24pril2010.. 2010, 868.
Yeung K.W.K., Lam K.O., Luk K.D.K., Cheung K.M.C., Wu S.L., Hu T., Liu X.Y., Chu C.L., Chu P.K. and Chan D., Long Term Cytocompatibility and In vivo Investigation of Nitrogen Plasma Implanted Shape Memory Alloy. , International Conference on Shape Memory ands Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 104-105.


Researcher : Lam RWM

List of Research Outputs

Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Pan H., Li Z., Chen Y., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Fatty acid affects the nucleation of strontium-substituted biominerals, Ceram Int. 2010, 36(2): 683-688.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Li Z., Lu W.W., Deng L., Chiu P.K.Y., Fang D., Lam R.W.M., Leong J.C.Y. and Luk K.D.K., The morphology and lattice structure of bone crystal after strontium treatment in goats, Journal Of Bone And Mineral Research. 2010, 28: 25-34.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Zhao X., Li Z., Liu W.G., Pan H., Lam R.W.M., Luk K.D.K. and Lu W.W., Characterization and evaluation of octa-arginine conjugated chitosan as non-biral vector for gene transfer, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Lam SKL

List of Research Outputs

Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.
Lam S.K.L., Luk K.D.K. and Lu W.W., Biomechanics of the intervertebral disc allograft transplantation , 2010.
Lam S.K.L., Luk K.D.K., Ruan D.K., Ding Y. and Lu W.W., In-vivo kinematics of the IVD allograft transplantation, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lam S.K.L., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., In-vivo kinematics of the intervertebral disc allograft transplantation, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Lam S.K.L., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., The effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Lam S.K.L., Luk K.D.K., Xiao J., Ruan D.K., Ding Y. and Lu W.W., The kinematics of the mal-placement of the intervertebral disc allograft transplant on the segmental mobility, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Luk K.D.K., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Lam S.K.L., Effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.


Researcher : Lam SKL

List of Research Outputs

Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.
Lam S.K.L., Luk K.D.K. and Lu W.W., Biomechanics of the intervertebral disc allograft transplantation , 2010.
Lam S.K.L., Luk K.D.K., Ruan D.K., Ding Y. and Lu W.W., In-vivo kinematics of the IVD allograft transplantation, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lam S.K.L., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., In-vivo kinematics of the intervertebral disc allograft transplantation, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Lam S.K.L., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., The effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Lam S.K.L., Luk K.D.K., Xiao J., Ruan D.K., Ding Y. and Lu W.W., The kinematics of the mal-placement of the intervertebral disc allograft transplant on the segmental mobility, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Luk K.D.K., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Lam S.K.L., Effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.


Researcher : Lam TK

List of Research Outputs

Lee K.S., Lam T.K., Song Y., Cheah K.S.E., Cheung K.M.C. and Chan D., In vivo study of Asporin, a genetic risk factor for Osteoarthritis and degenerative disc disease, 2010 Hong Kong Inter-University Biochemistry Postgraduate Symposium, CUHK, Hong Kong, 15 May,. 2010.


Researcher : Lam WMR

List of Research Outputs

Lam W.M.R., Pan H., Li Z., Yang C., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Strontium-substituted calcium phosphates prepared by hydrothermal method under linoleic acid-ethanol solution, CERAMICS INTERNATIONAL. 2010, 36(2): 683-688.
Pan H., Lam W.M.R., Zhao X., Li Z., Lu W.W., Wang D., Huang W., Lin K. and Chang J., Borosilicate for tissue engineering, World Association for Chinese Biomedical Engineers (WACBE). 2009.
Tong H.W., Wang M., Li Z., Lam W.M.R. and Lu W.W., Electrospinning of surface-porous fibers and In vitro biological study of these fibers, Proceedings of WACBE World Congress on Bioengineering 2009. Hong Kong, 38.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.


Researcher : Lam WN

List of Research Outputs

Yeung K.W.K., Lam W.N., Natarajan D., Wu S.L., Hu T., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Nickel Ion Level in Scoliotic Patients Implanted with Nitrogen Plasma Surface Modified Nickel-Titanium Superelastic Spinal Implant., 3rd IEEE International NanoElectronics Conference (INEC), Hong Kong, 3-8 January 2010. Oral presentation.. 2010, Paper BC410, p. 134.
Yeung K.W.K., Lam W.N., Wu S.L., Tu H., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Post-Operative Monitoring of Nickel Ion Level in Scoliotic Patients Operated with Novel Nickel-Titanium Superelastic Spinal Implant. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20 May 2010). 2010, 22-23.


Researcher : Lau TW

List of Research Outputs

Kwan K., Lau T.W. and Leung F.K.L., Operative treatment of distal radial fractures with locking plate system – a prospective study International Orthopaedics 2010 (doi 10.1007/s00264-010, 2010.
Lau T.W., Leung F.K.L. and Lu Y.G., Application of Minimally Invasive Locking Compression Plate in the treatment of proximal humeral fractures Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1282-84, 2009.
Lau T.W. and Leung F.K.L., Osteoporotic pelvic fractures : Diagnosis and Management , SICOT/SIROT 2008 XXIV Triennial World Congress, Hong Kong, 24-28 Aug,2008. 2009.
Lau T.W. and Leung F.K.L., Prospective study of the use of new dynamic hip screw-blade system in trochanteric fractures 29th Annual Congress, Hong Kong Orthopaedic Association, Hong Kong, 28-29 Nov, 2009, 2009.
Leung F.K.L. and Lau T.W., Application of minimally invasive locking compression plate in treatment of distal tibia fractures Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1323-25, 2009.
Loong H.N.C., Chan Y.Y., Lau T.W., Leung F.K.L. and Kung A.W.C., A Secondary Fracture Prevention Programme to Reduce Fractures, Hospital Admissions, and Mortality Rates at One and Five Years Osteoporosis Society of Hong Kong, 11th Regional Osteoporosis Conference, Hong Kong, 15-16 May, 2010, 2010.
Loong H.N.C., Chan Y.Y., Lau T.W., Leung F.K.L., Luk K.D.K. and Kung A.W.C., Evaluation of the Osteoporosis Secondary Fracture Prevention Program at Queen Mary Hospital: Successful Recruitment is associated with Lower Re-fracture and Mortality Rates at One and Five years Osteoporosis Society of Hong Kong, 11th Regional Osteoporosis Conference, Hong Kong, 15-16 May, 2010, 2010.
Yuen W.Y.G., Lau T.W. and Leung F.K.L., A Cadaveric study of Asian distal radius morphoanatomy in the application of volar locking plate fixation Poster presentation at the Orthopedic Research Society Meeting, New Orleans, USA, 6-9 March, 2010, USA, 2010.
Zhou Z.G., Kwan K., Lau T.W. and Leung F.K.L., Clinical effects of locking compression plate system in treating distal radial fractures Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1294-97, 2009.


Researcher : Lau WL

List of Research Outputs

Chiu P.K.Y., Ng F.Y., Lee O.B., Tsang W.L., Wong H.L., Leung K.H., Wu W.C., Tang W.M., Chan W.S. and Lau W.L., A prospective, randomized, inter-regional, multi-centre study on gender solutions knee prostheses in Chinese females, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.


Researcher : Lee OB

List of Research Outputs

Chiu P.K.Y., Ng F.Y., Lee O.B., Tsang W.L., Wong H.L., Leung K.H., Wu W.C., Tang W.M., Chan W.S. and Lau W.L., A prospective, randomized, inter-regional, multi-centre study on gender solutions knee prostheses in Chinese females, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.


Researcher : Lee YE

List of Research Outputs

Ip W.Y., Fung B.K.K. and Lee Y.E., Mycobacterium Chelonae Hand Infection:a Review Of Hand Sugery, Edmond Ted U. 2009, 14 (1): 7-13.
Ip W.Y. and Lee Y.E., Necrotizing Fasciitis Of The Extremity Caused By Haemophilus Influenzae Serotype B In A Healthy Adult, Clin Orthop Relat Res. 2010, 468(5): 1436-9.


Researcher : Leong JCY

List of Research Outputs

Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Canada patent no. 2,395,910, granted on September 22, 2009, 2009.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Hong Kong Standard patent no. HK1046378, granted on July 17, 2009, 2009.
Li Z., Lu W.W., Deng L., Chiu P.K.Y., Fang D., Lam R.W.M., Leong J.C.Y. and Luk K.D.K., The morphology and lattice structure of bone crystal after strontium treatment in goats, Journal Of Bone And Mineral Research. 2010, 28: 25-34.
Ming D., Bai Y., Liu X., Qi H., Cheng L., Wan B., Hu Y., Wong Y.W., Luk K.D.K. and Leong J.C.Y., A gait stability investigation into FES-assisted paraplegic walking based on the walker tipping index. , J Neural Eng. 2009, 6: 6600-7.
Ming D., Hu Y., Wong Y.W., Wan B.K., Luk K.D.K. and Leong J.C.Y., Risk-tendency graph (RTG): a new gait-analysis technique for monitoring FES-assisted paraplegic walking stability., Med Sci Monit. . 2009, 15: 105-12.


Researcher : Leung FKL

Project Title:Effects of Alendronate on TGF Beta Signaling: An In Vitro Study with Co-culture of Osteoblastic and Osteoclastic Cell Lines
Investigator(s):Leung FKL, Lu WW, Chan D
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:10/2009
Abstract:
Bisphosphonates (BPs) are potent anti-resorptive agents and their therapeutic use in osteoporosis treatment and fracture prevention has been widely established1-3. However, the paradigm for drug discovery which requires identification of a target involved in the disease process (e.g. enzyme or receptor) and the development of an appropriate ligand (activator, inhibitor or selective modulator)4 has not been followed despite its prevalence in prescription and administration. BPs are analogs of pyrophosphate (P-O-P) in which the germinal oxygen has been substituted by carbon (P-C-P) that bind to hydroxyapatite found in bone4. BPs are inhibitors of bone resorption by reducing the recruitment and activity of osteoclasts as well as enhancing apoptosis of osteoclasrs5,6. BPs are rapidly cleared from the circulation and due to their hydrophilic phosphonate property, limits the penetration through cellular lipid bilayer membranes, thus distribution of BPs is confined to bone. Most of the published findings focused on clinical efficacy suggesting increased bone mineral density and the resultant reduction of fracture risk after BP treatment7-26. At the molecular level, nitrogen-containing BPs (N-BPs) inhibit farnesyl diphosphate (FPP) synthase in the mevalonate pathway of cholesterol synthesis, resulting in a decrease of prenylation of small GTPases, those are vital in regulating cell processes required for osteoclast function, including cytoskeletal regulation, ruffle border formation and apoptosis control4, 27. Whereas, the most probable mechanism of non-nitrogen-containing BPs (non-N-BPs) involves the incorporation of the P-C-P backbone into β and γ positions of adenosine triphosphate (ATP) and BPs will be converted to toxic ATP metabolite, AppCCl2p. It substitutes for pyrophosphate in the reverse the reaction of ATP-dependent tRNA formation, thus leading to cell death4, 27. After mechanism of action from BPs on osteoclasts being condignly addressed, there were several in-vitro studies reporting inconsistent effects of BPs on osteoblasts. the results are incomparable due to variations in BPs, outcome measures and cell lines selections1, 27-31. But, a significant finding of BPs act on osteoclastic bone resorption through mediation of osteoblasts had been reported by Sahni et al in 199332. Transforming growth factor-beta (TGF-β) is abundant in bone matrix which plays a critical role in bone remodeling. Bone remodeling depends on the spatial and temporal coupling of bone formation by osteoblasts and bone resorption by osteoclasts33-35. TGF-β is the core of osteoblastic-osteoclastic coupling36-37 in bone formation and resorption; hence, it is a key in etiology of osteoporosis. In another study, the sequence variation, 713–8delC in the TGF-β1 gene was found to be associated with very low bone mass in osteoporotic women36, 38; with increased bone turnover in both osteoporotic and normal women38; and prevalence of osteoporotic fractures36. On top, Erlebacher & Derynck found increased expression of TGF-β2 in osteoblasts results in an osteoporosis-like phenotype39. On the other hand, inhibition of TGF-β type II receptor signaling in osteoblasts was shown to increase trabecular bone mass caused by unaltered osteoblastic bone formation with decreased bone resorption by osteoclasts, in an in-vivo study with transgenic mice40. This is similar to vivo/clinical findings after BPs treatment. In summary, TGF-β is essential in osteoblastic-osteoclastic coupling in bone formation-resorption and a possible contributing factor to osteoporosis. The cause of inconsistency among previous research findings on effects of BPs on osteoblasts remains unidentified. But, single cell culture should not be a topic being disregarded since bone formation-reorption is coupled with osteoclasts. Purpose of Study With unknown impact from bisphosphonates on osteoblasts molecularly, we would like to propose an in-vitro study to explore effects of alendronate, the commonest N-BP in market, on osteoblasts with active TGF-β in co-culture of osteoclastic and osteoblastic cell lines. Objectives 1)To evaluate the effects of alendronate with active TGF-β 1/2/3 on osteoblast proliferation, osteogenic differentiation and gene expression 2) To analyse the influence of alendronate with active TGF-β 1/2/3 on the osteoblast cell-surface expression of individual TGF-βR proteins


List of Research Outputs

Chan C.F., Yuen W.Y.G. and Leung F.K.L., Fixation of distal humeral fracture in elderly patient by locking compression plate Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1285-89, 2009.
Chow S.P. and Leung F.K.L., Chow SP, Leung F (2010): Radial and Ulnar Shaft fractures in Rockwood and Green’s Fractures in adults, Lippincott Williams & Wilkins; 7th edition: 881-904, USA, Lippincott Williams & Wilkins, 2010.
Fok M.W.M., Leung H.B. and Leung F.K.L., Cortex-medulla width ratio as a diagnostic means for adynamic bone disease 29th Annual Congress, Hong Kong Orthopaedic Association, Hong Kong, 28-29 Nov, 2009, 2009.
Kwan K., Lau T.W. and Leung F.K.L., Operative treatment of distal radial fractures with locking plate system – a prospective study International Orthopaedics 2010 (doi 10.1007/s00264-010, 2010.
Lau T.W., Leung F.K.L. and Lu Y.G., Application of Minimally Invasive Locking Compression Plate in the treatment of proximal humeral fractures Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1282-84, 2009.
Lau T.W. and Leung F.K.L., Osteoporotic pelvic fractures : Diagnosis and Management , SICOT/SIROT 2008 XXIV Triennial World Congress, Hong Kong, 24-28 Aug,2008. 2009.
Lau T.W. and Leung F.K.L., Prospective study of the use of new dynamic hip screw-blade system in trochanteric fractures 29th Annual Congress, Hong Kong Orthopaedic Association, Hong Kong, 28-29 Nov, 2009, 2009.
Leung F.K.L., 160. Principles of open fracture management AO Course, Principles of Operative Fracture Management, Shenyang, China, Sept 22-24, 2009, 2009.
Leung F.K.L., 161. Principles of treatment of articular fractures AO Course, Principles of Operative Fracture Management, Shenyang, China, Sept 22-24, 2009, 2009.
Leung F.K.L., Advances in femoral shaft IM nailing Intramedullary Nailing Symposium, Pre-congress symposium, Xiamen, China, 19 Nov, 2009, 2009.
Leung F.K.L., Advances in proximal tibial plating Satellite symposium, 4th Chinese Orthopaedic Association Meeting, Xiamen, China, 19 Nov, 2009, 2009.
Leung F.K.L., Advances in tibial nailing Intramedullary Nailing Symposium, Pre-congress symposium, Xiamen, China, 19 Nov, 2009, 2009.
Leung F.K.L., Anatomical consideration in design of distal radius plating 16th CUHK-AADO-Stryker Advanced Trauma Course, Hong Kong, 5-6 June, 2010, 2010.
Leung F.K.L. and Lau T.W., Application of minimally invasive locking compression plate in treatment of distal tibia fractures Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1323-25, 2009.
Leung F.K.L. and Yuen W.Y.G., Cadaveric study of Asian distal radius morphoanatomy in the application of volar locking plate fixation The 4th Chinese Orthopaedic Association Meeting, Xiamen, China, 19-22 November, 2009 , 2009.
Leung F.K.L., Choice of fixation in intertrochanteric fractures Orthopaedic Symposium, Gweizhou Hospital, Shunde, China 23 January, 2010, 2010.
Leung F.K.L., Co-managed Care in Geriatric Hip fractures The First Asian Federation of Osteoporosis Society Scientific Meeting, Guangzhou, China, Nov 13-14, 2009, 2009.
Leung F.K.L., Complex elbow fracture dislocations Gurajat Orthopaedic Association Congress, Surat, India, 5-7 Feb, 2010, 2010.
Leung F.K.L., Complications and pitfalls in the application of locking plates and how to avoid 16th CUHK-AADO-Stryker Advanced Trauma Course, Hong Kong, 5-6 June, 2010, 2010.
Leung F.K.L., Development of locking plates 16th CUHK-AADO-Stryker Advanced Trauma Course, Hong Kong, 5-6 June, 2010, 2010.
Leung F.K.L., Distal femur: Clinical issues with nailing Synthes ‘Meet the Experts’ seminar, Suzhou, China, 18-19 May , 2010, 2010.
Leung F.K.L., Distal femur: Clinical issues with nailing 4th AO Experts Symposium on IM nailing and plating, Shanghai, China, 22-23 May , 2010, 2010.
Leung F.K.L., Distal radius ORIF- volar approach 3rd AO Asia-Pacific Experts’ Seminar , 11 July 2009, 2009.
Leung F.K.L., Distal radius fractures Gurajat Orthopaedic Association Congress, Surat, India, 5-7 Feb, 2010, 2010.
Leung F.K.L., Distal tibia: Clinical issues with nailing 4th AO Experts Symposium on IM nailing and plating, Shanghai, China, 22-23 May , 2010, 2010.
Leung F.K.L., Editorial board member, Journal of Orthopaedic Surgery (Official Journal of Korean Orthopaedic Association. South Korea, 2009.
Leung F.K.L., Femoral Shaft Fractures: Controversies on IM nailing AOTrauma Course, Advances in Operative Fracture Management, Nanjing, China, 14-16 May , 2010, 2010.
Leung F.K.L., Fractures of the Calcaneus AOTrauma Course, Advances in Operative Fracture Management, Nanjing, China, 14-16 May , 2010, 2010.
Leung F.K.L., Infection after osteosynthesis AO Course, Principles of Operative Fracture Management, Shanghai, China, Sept 18-20, 2009, 2009.
Leung F.K.L., Infection after osteosynthesis AO Course, Principles of Operative Fracture Management, Shenyang, China, Sept 22-24, 2009, 2009.
Leung F.K.L., Injuries to acromioclavicular joint Gurajat Orthopaedic Association Congress, Surat, India, 5-7 Feb, 2010, 2010.
Leung F.K.L., Intramedullary Nailing AO Course, Principles of Operative Fracture Management, Shenyang, China, Sept 22-24, 2009, 2009.
Leung F.K.L., Intramedullary Nailing AO Course, Priniciples of Operative Fracture management, Shanghai, China, Sept 18-20,2009, 2009.
Leung F.K.L., Locked compression plate- why and what for AO Course, Principles of Operative Fracture Management, Shanghai, China, Sept 18-20, 2009, 2009.
Leung F.K.L., Management of Open fracture Post-registration Certificate Course in Orthopaedics and Traumatology Nursing, Hong Kong, 18 June, 2010, 2010.
Leung F.K.L., Management of pilon fractures: modern plating techniques Gurajat Orthopaedic Association Congress, Surat, India, 5-7 Feb, 2010, 2010.
Leung F.K.L., Management of sacral fractures Gurajat Orthopaedic Association Congress, Surat, India, 5-7 Feb, 2010 , 2010.
Leung F.K.L., Minimally Invasive Surgery- Is it the Future? AOTrauma Course, Advances in Operative Fracture Management, Nanjing, China, 14-16 May , 2010, 2010.
Leung F.K.L., Nonunion in upper extremity The 23rd Hong Kong Society For Surgery of the Hand (HKSSH) Annual congress, Hong Kong, 20-21 Mar, 2010, 2010.
Leung F.K.L., Pitfalls in treating ankle fractures Sixth SICOT/SIROT Annual International Conference, Combined Meeting with the Royal College of Orthopaedic Surgeons of Thailand (RCOST), Pattaya, Thailand, Oct 29-Nov 1, 2009, 2009.
Leung F.K.L., Plate fixation of distal radius fractures 29th Annual Congress, the Hong Kong Orthopaedic Association, Hong Kong, 28-29 Nov, 2009, 2009.
Leung F.K.L., Principles and Techniques of Osteosynthesis in Fractures of the Acetabulum The Acetabular Fracture, edited by L Maini, A Dogra: 38-43 , 2009.
Leung F.K.L., Principles of open fracture management AO Course, Principles of Operative Fracture Management, Shanghai, China, Sept 18-20, 2009, 2009.
Leung F.K.L., Principles of treatment of articular fractures AO Course, Principles of Operative Fracture Management, Shanghai, China, Sept 18-20, 2009, 2009.
Leung F.K.L., Proximal femoral nailing Proximal femoral nailing Symposium, Philippine Orthopedic Center, Manila, Oct 16-17, 2009, 2009.
Leung F.K.L., Proximal femoral nailing Intramedullary Nailing Symposium, Pre-congress symposium, Xiamen, China, 19 Nov, 2009, 2009.
Leung F.K.L., Proximal humerus- fixation with LCP pearls and pitfalls 3rd AO Asia-Pacific Experts’ Seminar , 11 July 2009, 2009.
Leung F.K.L., Reduction of fractures with minimally invasive techniques AO Course- Advances in paediatric fractures and minimally invasive osteosynthesis, Hong Kong, 29-31 January, 2010, 2010.
Leung F.K.L., Reduction techniques AO Course, Principles of Operative Fracture Management, Shenyang, China, Sept 22-24, 2009, 2009.
Leung F.K.L., Reduction techniques AO Course, Principles of Operative Fracture Management, Shanghai, China, Sept 18-20,2009, 2009.
Leung F.K.L., Repair and Reconstruction of Bone Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1281, 2009.
Leung F.K.L., The Unstable Elbow Dislocation AOTrauma Course, Advances in Operative Fracture Management, Nanjing, China, 14-16 May , 2010, 2010.
Leung F.K.L., Updates in the management of osteoporotic fractures Hong Kong Academy of Medicine - CME lecture, Hong Kong, 14 Apr, 2010, 2010.
Leung F.K.L., Wrist fractures in older patients: What counts Plenary lectures, 29th Annual Congress, the Hong Kong Orthopaedic Association, Hong Kong, 28-29 Nov, 2009 , 2009.
Loong H.N.C., Chan Y.Y., Lau T.W., Leung F.K.L. and Kung A.W.C., A Secondary Fracture Prevention Programme to Reduce Fractures, Hospital Admissions, and Mortality Rates at One and Five Years Osteoporosis Society of Hong Kong, 11th Regional Osteoporosis Conference, Hong Kong, 15-16 May, 2010, 2010.
Loong H.N.C., Chan Y.Y., Lau T.W., Leung F.K.L., Luk K.D.K. and Kung A.W.C., Evaluation of the Osteoporosis Secondary Fracture Prevention Program at Queen Mary Hospital: Successful Recruitment is associated with Lower Re-fracture and Mortality Rates at One and Five years Osteoporosis Society of Hong Kong, 11th Regional Osteoporosis Conference, Hong Kong, 15-16 May, 2010, 2010.
Yuen W.Y.G. and Leung F.K.L., A Cadaveric study of Asian distal radius morphoanatomy in the application of volar locking plate fixation, 2009.
Yuen W.Y.G., Lau T.W. and Leung F.K.L., A Cadaveric study of Asian distal radius morphoanatomy in the application of volar locking plate fixation Poster presentation at the Orthopedic Research Society Meeting, New Orleans, USA, 6-9 March, 2010, USA, 2010.
Yuen W.Y.G. and Leung F.K.L., Best basic science paper: A cadaveric study of anatomical landmarks and guidelines for volar locking plate application in the distal radius , The Hong Kong Orthopaedic Association Annual Congress 2009. 2009.
Zhou Z.G., Kwan K., Lau T.W. and Leung F.K.L., Clinical effects of locking compression plate system in treating distal radial fractures Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1294-97, 2009.


Researcher : Leung HB

List of Research Outputs

Leung H.B., Cheung K.M.C., Lu W.W., Tseng H. and Luk K.D.K., The effect of insertion timing on pullout strength of pedicle screws from polymethylmethacrylate bone cement, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Leung KL

Project Title:Effects of Alendronate on TGF Beta Signaling: An In Vitro Study with Co-culture of Osteoblastic and Osteoclastic Cell Lines
Investigator(s):Leung FKL, Lu WW, Chan D
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:10/2009
Abstract:
Bisphosphonates (BPs) are potent anti-resorptive agents and their therapeutic use in osteoporosis treatment and fracture prevention has been widely established1-3. However, the paradigm for drug discovery which requires identification of a target involved in the disease process (e.g. enzyme or receptor) and the development of an appropriate ligand (activator, inhibitor or selective modulator)4 has not been followed despite its prevalence in prescription and administration. BPs are analogs of pyrophosphate (P-O-P) in which the germinal oxygen has been substituted by carbon (P-C-P) that bind to hydroxyapatite found in bone4. BPs are inhibitors of bone resorption by reducing the recruitment and activity of osteoclasts as well as enhancing apoptosis of osteoclasrs5,6. BPs are rapidly cleared from the circulation and due to their hydrophilic phosphonate property, limits the penetration through cellular lipid bilayer membranes, thus distribution of BPs is confined to bone. Most of the published findings focused on clinical efficacy suggesting increased bone mineral density and the resultant reduction of fracture risk after BP treatment7-26. At the molecular level, nitrogen-containing BPs (N-BPs) inhibit farnesyl diphosphate (FPP) synthase in the mevalonate pathway of cholesterol synthesis, resulting in a decrease of prenylation of small GTPases, those are vital in regulating cell processes required for osteoclast function, including cytoskeletal regulation, ruffle border formation and apoptosis control4, 27. Whereas, the most probable mechanism of non-nitrogen-containing BPs (non-N-BPs) involves the incorporation of the P-C-P backbone into β and γ positions of adenosine triphosphate (ATP) and BPs will be converted to toxic ATP metabolite, AppCCl2p. It substitutes for pyrophosphate in the reverse the reaction of ATP-dependent tRNA formation, thus leading to cell death4, 27. After mechanism of action from BPs on osteoclasts being condignly addressed, there were several in-vitro studies reporting inconsistent effects of BPs on osteoblasts. the results are incomparable due to variations in BPs, outcome measures and cell lines selections1, 27-31. But, a significant finding of BPs act on osteoclastic bone resorption through mediation of osteoblasts had been reported by Sahni et al in 199332. Transforming growth factor-beta (TGF-β) is abundant in bone matrix which plays a critical role in bone remodeling. Bone remodeling depends on the spatial and temporal coupling of bone formation by osteoblasts and bone resorption by osteoclasts33-35. TGF-β is the core of osteoblastic-osteoclastic coupling36-37 in bone formation and resorption; hence, it is a key in etiology of osteoporosis. In another study, the sequence variation, 713–8delC in the TGF-β1 gene was found to be associated with very low bone mass in osteoporotic women36, 38; with increased bone turnover in both osteoporotic and normal women38; and prevalence of osteoporotic fractures36. On top, Erlebacher & Derynck found increased expression of TGF-β2 in osteoblasts results in an osteoporosis-like phenotype39. On the other hand, inhibition of TGF-β type II receptor signaling in osteoblasts was shown to increase trabecular bone mass caused by unaltered osteoblastic bone formation with decreased bone resorption by osteoclasts, in an in-vivo study with transgenic mice40. This is similar to vivo/clinical findings after BPs treatment. In summary, TGF-β is essential in osteoblastic-osteoclastic coupling in bone formation-resorption and a possible contributing factor to osteoporosis. The cause of inconsistency among previous research findings on effects of BPs on osteoblasts remains unidentified. But, single cell culture should not be a topic being disregarded since bone formation-reorption is coupled with osteoclasts. Purpose of Study With unknown impact from bisphosphonates on osteoblasts molecularly, we would like to propose an in-vitro study to explore effects of alendronate, the commonest N-BP in market, on osteoblasts with active TGF-β in co-culture of osteoclastic and osteoblastic cell lines. Objectives 1)To evaluate the effects of alendronate with active TGF-β 1/2/3 on osteoblast proliferation, osteogenic differentiation and gene expression 2) To analyse the influence of alendronate with active TGF-β 1/2/3 on the osteoblast cell-surface expression of individual TGF-βR proteins


List of Research Outputs



Researcher : Leung KY

List of Research Outputs

Yeung C.Y., Leung K.Y., Yeung K.W.K., Kao R.Y.T., Chu P.K., Cheung K.M.C. and Luk K.D.K., Inhibition of metallic implant-related bacterial infections by novel water plasma surface treatment, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Leung K.Y., Kao R.Y.T., Chu P.K., Yip K., Luk K.D.K. and Cheung K.M.C., Feasibility Study of a New Plasma Coating to Suppress Bacterial Adhesion in Orthopaedic, Proceedings 4th International Conference on Technological Advances of Thin Films and Surface Coatings (ThinFilms 2008) (July 13-16, 2008). 2009, (Paper 4150) 35.


Researcher : Leung KY

List of Research Outputs

Yeung C.Y., Leung K.Y., Yeung K.W.K., Kao R.Y.T., Chu P.K., Cheung K.M.C. and Luk K.D.K., Inhibition of metallic implant-related bacterial infections by novel water plasma surface treatment, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Leung K.Y., Kao R.Y.T., Chu P.K., Yip K., Luk K.D.K. and Cheung K.M.C., Feasibility Study of a New Plasma Coating to Suppress Bacterial Adhesion in Orthopaedic, Proceedings 4th International Conference on Technological Advances of Thin Films and Surface Coatings (ThinFilms 2008) (July 13-16, 2008). 2009, (Paper 4150) 35.


Researcher : Leung YL

List of Research Outputs

Aladin K.D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nanostructure of Collagen Fibrils in Human Nucleus Pulposus and Its Correlation with Macroscale Tissue Mechanics, J. Ortho. Res.. 2009, 28: 497-502.
Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nano-structure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics, Journal of Orthopedic Research. 2010, 28: 497-502.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow-derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486-496.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow–derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486–496.
Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, Orthopaedic Research Society. 2010.
Chan K.Y., Leung Y.L., Tam V., Lu W.W., Sze K.Y. and Cheung K.M.C., Production of a natural disc scaffold for intervertebral disc tissue engineering, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Leung Y.L., Tsui Y.K., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 55th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA. 2010.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Leung Y.L., Gao B., Melhado I.G., Leung K.K.H., Wynn S.L., Chan D. and Cheah K.S.E., Sox9 mediates gene expression specificity in growth plate chondrocytes via concomitant positive and negative regulation, 55th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA. 2010.
Leung Y.L., Webster Jee Young Investigator Award "Sox9 mediates gene expression specificity in growth plate chondrocytes via concomitant positive and negative regulation", 15th Annual meeting of International Chinese Hard Tissue Society. 2010.
Lu W.W., Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T. and Luk K.D.K., Correlation between the nano-structure and macro-mechanics of human nucleus pulposus, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Tsui Y.K., Leung Y.L., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Tsui Y.K., Cheung K.M.C., Leung Y.L., Chan D. and Kao R.Y.T., Uncovering new compounds for treatment of interveretebral disc degeneration by chemical genetics, 2010.
Wong H.M., Yeung K.W.K., Leung Y.L., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., In-vitro analysis of gene expressions of osteoblastic cells while exposing to various concentrations of magnesium ions, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., 250. Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009, 17: 1959-1966.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009.


Researcher : Leung YL

List of Research Outputs

Aladin K.D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nanostructure of Collagen Fibrils in Human Nucleus Pulposus and Its Correlation with Macroscale Tissue Mechanics, J. Ortho. Res.. 2009, 28: 497-502.
Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nano-structure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics, Journal of Orthopedic Research. 2010, 28: 497-502.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow-derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486-496.
Chan C.W., Gantenbein-Ritter B., Leung Y.L., Chan D., Cheung K.M.C. and Ito K., Cryopreserved intervertebral disc with injected bone marrow–derived stromal cells: a feasibility study using organ culture, The Spine Journal. 2010, 10: 486–496.
Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, Orthopaedic Research Society. 2010.
Chan K.Y., Leung Y.L., Tam V., Lu W.W., Sze K.Y. and Cheung K.M.C., Production of a natural disc scaffold for intervertebral disc tissue engineering, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Leung Y.L., Tsui Y.K., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 55th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA. 2010.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Leung Y.L., Gao B., Melhado I.G., Leung K.K.H., Wynn S.L., Chan D. and Cheah K.S.E., Sox9 mediates gene expression specificity in growth plate chondrocytes via concomitant positive and negative regulation, 55th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA. 2010.
Leung Y.L., Webster Jee Young Investigator Award "Sox9 mediates gene expression specificity in growth plate chondrocytes via concomitant positive and negative regulation", 15th Annual meeting of International Chinese Hard Tissue Society. 2010.
Lu W.W., Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T. and Luk K.D.K., Correlation between the nano-structure and macro-mechanics of human nucleus pulposus, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Tsui Y.K., Leung Y.L., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Tsui Y.K., Cheung K.M.C., Leung Y.L., Chan D. and Kao R.Y.T., Uncovering new compounds for treatment of interveretebral disc degeneration by chemical genetics, 2010.
Wong H.M., Yeung K.W.K., Leung Y.L., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., In-vitro analysis of gene expressions of osteoblastic cells while exposing to various concentrations of magnesium ions, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., 250. Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009, 17: 1959-1966.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009.


Researcher : Li J

List of Research Outputs

Cheung K.M.C., Samartzis D., Natarajan D., Li J., Cheung W.Y., Lenke L.G. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Spine Chapter's Most Promising Spine Paper Award. Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Li J., Cheung K.M.C., Samartzis D., Zhu X.D., Li M. and Luk K.D.K., Key-Vertebral Screw Strategy (KVSS): an alternative surgical strategy for main thoracic curve correction in patients with adolescent idiopathic scoliosis, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009 . 2009.


Researcher : Li TH

List of Research Outputs

Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Multiple Bioimaging Modalities Application for Chronic Compressive Spinal Cord of Rat. , the 54th Annual Meeting of the Orthopedic Research Society. New Orleans, Louisiana; 2010.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Ultrastructural imaging of Cervical Spinal Cord with Induced Compressive Injuries in a Rat Model., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Li T.H., Luk K.D.K., Hu Y. and Wu E.X., Ultrastructural imaging of the cervical spinal cord , 2010.
Wen C., Hu Y., Li T.H., Lam K.O., Wu E.X. and Luk K.D.K., Multiple bio-imaging evaluations of spinal cord chronic compression in rat, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Li TH

List of Research Outputs

Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Multiple Bioimaging Modalities Application for Chronic Compressive Spinal Cord of Rat. , the 54th Annual Meeting of the Orthopedic Research Society. New Orleans, Louisiana; 2010.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Ultrastructural imaging of Cervical Spinal Cord with Induced Compressive Injuries in a Rat Model., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Li T.H., Luk K.D.K., Hu Y. and Wu E.X., Ultrastructural imaging of the cervical spinal cord , 2010.
Wen C., Hu Y., Li T.H., Lam K.O., Wu E.X. and Luk K.D.K., Multiple bio-imaging evaluations of spinal cord chronic compression in rat, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Li YH

List of Research Outputs

To M.K.T., Chow W., Li Y.H. and Eid M., Long-term efficacy of orthotic devices in children with flexible pes planus, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Li Z

Project Title:Peptide functionalized chitosan as nonviral vector for mediating gene therapy
Investigator(s):Li Z, Lu WW, Liu WG, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Small Project Funding
Start Date:01/2009
Abstract:
In Hong Kong, the number of osteoporotic fracture cases is ever increasing and it was ranked among the top-five conditions causing disability, which prolonged the hospital stay of such patients. The cost for treatment is expected to increase 3 times by 2010 as compared to that in 1995 [1]. Unfortunately, the conventional fracture fixation techniques for treating osteoporotic bone fractures are unsatisfactory owing to the fragility and extremely low bone volume at the osteoporotic fracture sites. The bone morphogenetic proteins (BMPs) activity, such as BMP2, is required for the initiation of fracture healing [2]. However, the applications of BMPs have also been limited by the requirement of large doses and short-term bioavailability. Alternatively, gene therapy that was originally devised for the treatment of inherited genetic disorders provides an alternative method for the delivery of BMP protein to overcome these problems. The success of gene therapy is largely dependent on the development of a vector or vehicle that can selectively and efficiently deliver a gene to target cells with minimal toxicity. Viral vector based gene transfer is the most efficient technologies available today which exploit the natural ability of viruses to infect and deliver genes into animal cells. Viral vectors provide the best prospect of performing gene therapy at the present time, but their safety, such as immunogenicity and cytotoxicity, have limited the clinical usefulness of this method [3]. Nonviral vectors are promising, safe, therapeutic tool for BMPs gene delivery with sustainable mode in regenerative medicine. Chitosan, as a naturally occurring macromolecule, has been used to mediate gene delivery into various cell types [4,5]. Possessing positive charges, it can be complexed with negatively charged DNA [6]. Moreover, chitosan protects DNA from nuclease degradation [7,8]. However, as nonviral vector, it is safe to use but less efficient when comparing to viral vector. Cell-penetrating peptides modified chitosan increases cell penetrating function, whereas it is not stable because of enzyme degradation [9,10]. It is known that arginine residues play an important role in the translocation [11]. Arginine-rich peptide and oligoarginine are able to translocate through cell membrane very easily, and these cell penetrating peptides have been used as protein, DNA and oligoDNA carriers [12,13]. Octaarginine is the most effective among the oligoarginine [14], so we will develop octaarginine modified chitosan derivatives to increase the transfection efficiency and sustainability. Objectives: Develop a nonviral vector mediated gene therapy as the means of inducing bone regeneration at osteoporotic sites for osteoporotic fracture prevention and treatment. Key issues and deliverables: 1. Bone morphogenetic protein (BMP) has been shown to be effective in treating osteoporotic fractures, but the short half-life and the lack of a practical method for sustained delivery of these proteins have greatly limited the application of BMPs in clinics. 2. Chitosan is a promised nonviral vector for gene delivery, but it is less efficient when comparing to viral vector. 3. We will synthesize octaarginine modified chitosan as nonviral vector and deliver BMPs gene into C2C12 efficiently by applying this novel vector system. 4. The possible mechanism of improvement on transfection efficiency will be investigated in vitro. 5. Above investigations will provide information for optimization of the chitosan-based nonviral vector in terms of transfection efficiency, chemical properties, biocompatibility and efficacy on stimulating bone formation. References: 1. Lau EMC et al. (1997). Osteoporosis in Asia. World Scientific. 2. Tsuji K et al. Nat Genet. (2006) 38:1424-9. BMP2 activity, although dispensable for bone formation, is required for the initiation of fracture healing. 3. Glover, D.J., Lipps, H.J. & Jans, D.A. Nature Reviews Genetics (2005) 6, 299-310. Towards safe, non-viral therapeutic gene expression in humans.. 4. Liu WG et al. J Control Release (2002) 83:1–11.Chitosan-and its derivatives-a promising non-viral vector for gene transfection. 5. Corsi K et al. Biomaterials (2003) 24:1255-64. Mesenchymal stem cells, MG63 and HEK293 transfection using chitosan-DNA nanoparticles. 6. MacLaughlin FC et al. J Control Release. (1998) 56:259-72. Chitosan and depolymerized chitosan oligomers as condensing carriers for in vivo plasmid delivery. 7. Cui Z et al. J Control Release. (2001) 75:409-19. Chitosan-based nanoparticles for topical genetic immunization. 8. Illum L et al. Adv Drug Deliv Rev. (2001) 51:81-96. Chitosan as a novel nasal delivery system for vaccines. 9. Zorko M et al. Adv Drug Deliv Rev. (2005) 57:529-45. Cell-penetrating peptides: mechanism and kinetics of cargo delivery. 10. Chung SS et al. Int J Pharm. (2007), online published. Advances in cell-penetrating, non-peptide molecular carriers. 11. Khalil, I.A., Futaki, S., Niwa, M. & Baba, Y. Gene Ther. (2004) 11, 636-44. Mechanism of improved gene transfer by the N-terminal stearylation of octaarginine: enhanced cellular association by hydrophobic core formation. 12. Futaki, S. Adv Drug Deliv Rev (2005) 57, 547-58. Membrane-permeable arginine-rich peptides and the translocation mechanisms. 13. Rudolph, C., Plank, C., Lausier, J. & Schillinger, U. J. Biol. Chem. (2003) 278, 11411-8. Oligomers of the arginine-rich motif of the HIV-1 TAT protein arecapable of transferring plasmid DNA into cells. 14. Futaki, S., Ohashi, W., Suzuki, T. & Niwa, M. Bioconjugate Chem. (2001) 12, 1005-11 Stearylated arginine-rich peptides: A new class of transfection systems.


List of Research Outputs

Duan B., Wang M., Li Z. and Lu W.W., Bone morphogenetic protein incorporated nanocomposite scaffolds and induction of osteogenic differentiation of mesenchymal stem cells, Proceedings of the Tissue Engineering and Regenerative Medicine International Society – EU Meeting -2010. Galway, Ireland, Paper #459.
Duan B., Wang M., Li Z. and Lu W.W., Ca-P/PHBV nanocomposite scaffolds modified by gelatin and heparin and their biological evaluation, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 94.
Duan B., Wang M., Li Z. and Lu W.W., Cytocompatibility and osteoconductivity of three-dimensional selective laser sintered nanocomposite scaffolds, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 95.
Duan B., Wang M., Li Z. and Lu W.W., In Vitro Biological assessment of nanocomposite scaffolds for bone tissue engineering, Proceedings of WACBE World Congress on Bioengineering 2009. Hong Kong, 20.
Duan B., Wang M., Li Z. and Lu W.W., Surface modification of selective laser sintered nanocomposite scaffolds and osteogenic differentiation of mesenchymal stem cells on the scaffolds, Transactions of the Society For Biomaterials 2010 Annual Meeting. Seattle, WA, USA, 2010, Paper #421.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Pan H., Li Z., Chen Y., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Fatty acid affects the nucleation of strontium-substituted biominerals, Ceram Int. 2010, 36(2): 683-688.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Lam W.M.R., Pan H., Li Z., Yang C., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Strontium-substituted calcium phosphates prepared by hydrothermal method under linoleic acid-ethanol solution, CERAMICS INTERNATIONAL. 2010, 36(2): 683-688.
Li Z., Pan H., Luk K.D.K. and Lu W.W., Bone Mechanical Properties Determination from Macro- to Nanoscopic Scale, The 3rd China International Congress and Exibition of Orthopaedics. 2009.
Li Z., Luk K.D.K. and Lu W.W., Strontium Containing Bioactive Bone Cement for Orthopaedic Applications, In: S. C. Tjong, Advances in Biomedical Sciences and Engineering. Bentham, 2009, 69-81.
Li Z., Lu W.W., Deng L., Chiu P.K.Y., Fang D., Lam R.W.M., Leong J.C.Y. and Luk K.D.K., The morphology and lattice structure of bone crystal after strontium treatment in goats, Journal Of Bone And Mineral Research. 2010, 28: 25-34.
Luk K.D.K., Lu W.W., Zhou G., Peng S.L., Wang T. and Li Z., New Investigator Recognition Award., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010. 2010.
Ma W., Ng F.Y., Chiu P.K.Y., Li Z. and Lu W.W., The effects of acetabular component position and hip ball diameter on posterior stability after total hip replacement, Chinese Journal Of Joint Surgery. 2009, 3: 624-629.
Ni G., Lin J.H., Chiu P.K.Y., Li Z. and Lu W.W., Effect of strontium-containing hydroxyapatite bone cement on bone remodeling following hip replacement, Journal Of Material Science,. Materials In Medicine. 2010, 21: 377-384.
Pan H., Lam W.M.R., Zhao X., Li Z., Lu W.W., Wang D., Huang W., Lin K. and Chang J., Borosilicate for tissue engineering, World Association for Chinese Biomedical Engineers (WACBE). 2009.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Peng S.L., Liu X.W., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., Effect of different timing of strontium treatment on bone remodeling in ovariectomized rats, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Tong H.W., Wang M., Li Z. and Lu W.W., Effects of the alignment, diameter and surface morphology of electrospun fibers on the behaviour of osteoblastic cells, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 107.
Tong H.W., Wang M., Li Z., Lam W.M.R. and Lu W.W., Electrospinning of surface-porous fibers and In vitro biological study of these fibers, Proceedings of WACBE World Congress on Bioengineering 2009. Hong Kong, 38.
Tong H.W., Wang M., Li Z. and Lu W.W., In vitro biological assessment of electrospun composite fibers containing hydroxyapatite nanoparticles, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 108.
Tong H.W., Wang M., Li Z. and Lu W.W., The influence of fiber diameter, morphology and alignment on osteoblastic cell behaviour, Proceedings of the National Biomaterials Congress. Chengdu, China, 2010, CD-ROM: 1pp.
Tong H.W., Li Z., Wang M. and Lu W.W., 电纺纤维直径、表面形貌及排列状态对细胞的影响, 全国生物材料大会, 2010.
Zhao X., Li Z., Liu W.G., Pan H., Lam R.W.M., Luk K.D.K. and Lu W.W., Characterization and evaluation of octa-arginine conjugated chitosan as non-biral vector for gene transfer, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Lim CT

List of Research Outputs

Lu W.W., Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T. and Luk K.D.K., Correlation between the nano-structure and macro-mechanics of human nucleus pulposus, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.


Researcher : Liu H

List of Research Outputs

Hu Y., Liu H. and Luk K.D.K., Signal-to-Noise Ratio of Intraoperative Tibial Nerve Somatosensory-Evoked Potentials. , J Clin Neurophysiol.. 2010, 27: 30-3.
Hu Y., Liu H. and Luk K.D.K., Signal-to-noise ratio of intraoperative tibial nerve somatosensory-evoked potentials, Journal of Clinical Neurophysiology. 2010, 27(1): 30-33.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: Methods and comparisons. , J Electromyogr Kinesiol.. 2010, 20: 89-101.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: methods and comparisons, Journal of Electromyography and Kinesiology. 2010, 20: 89-101.


Researcher : Liu H

List of Research Outputs

Hu Y., Liu H. and Luk K.D.K., Signal-to-Noise Ratio of Intraoperative Tibial Nerve Somatosensory-Evoked Potentials. , J Clin Neurophysiol.. 2010, 27: 30-3.
Hu Y., Liu H. and Luk K.D.K., Signal-to-noise ratio of intraoperative tibial nerve somatosensory-evoked potentials, Journal of Clinical Neurophysiology. 2010, 27(1): 30-33.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: Methods and comparisons. , J Electromyogr Kinesiol.. 2010, 20: 89-101.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: methods and comparisons, Journal of Electromyography and Kinesiology. 2010, 20: 89-101.


Researcher : Liu WC

List of Research Outputs

Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.


Researcher : Liu WC

List of Research Outputs

Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.


Researcher : Lu WW

Project Title:Optimization and commercialization of strontium containing bioactive bone cement for various orthopaedic applications
Investigator(s):Lu WW, Chan WK, Xu B, Chiu PKY, Luk KDK, Ngan AHW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Guangdong-Hong Kong Technology Cooperation Funding Scheme
Start Date:04/2007
Abstract:
(1) Optimize and commercialize three new brands of Sr-HA bone cement to fulfill the application in three major areas: (i) Osteoporotic spinal fracture (Sr-HA Spine), (ii) Total hip replacement implant augmentation (Sr-HA Hip) and (iii) Bone tumor, or bone cavity filler (Sr-HA Filler). (2) Provide a complete mechancial and biocompatibility profile of the modified Sr-HA cement formulation as per the ISO 5883 and ISO 10993 standards. (3) Carry out 30-35 cases of clinical trials on bioactive bone cement as a total hip replacement fixation agent. (4) Filing the standards for application to local or international medical device licensing organizations for marketing. The key issue of this study is to optimize present Sr-HA bioactive bone cement by modifying its composition to fulfill a wied variety of orthopaedic application requirements while preserving its desired properties of bioactivity and osteointegration. Ultimately, we aim at replacing the current non-bioactive PMMA bone cement with bioactive bone cements suitable for the particular needs which can significantly reduce clincial complications and improve the quality of life among the patients.


Project Title:Guanidinylated chitosan-mediated gene transfected cell therapy to induce in situ bone regeneration
Investigator(s):Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Travel Grants for NSFC/RGC JRS
Start Date:12/2007
Abstract:
Travel grants for NSFC/RGC JRS.


Project Title:Functionalized multiwalled carbon nanotubes reinforced Sr-HA bioactive bone cement.
Investigator(s):Lu WW, Luk KDK, Wong CT
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:12/2007
Completion Date:06/2010
Abstract:
(1) To functionalize the multiwalled carbon nanotubes (MWNT) with double bond containing compounds that can be crosslinked with the cement. (2) To develop a reinforced Sr-HA bioactive bone cement. (3) To re-evaluate the mechanical properties and biocompatibility of the developed Sr-HA bioactive bone cement.


Project Title:Developing of Sr based small molecular biomaterials for bone repair and mechanism study
Investigator(s):Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Travel Grants for NSFC/RGC JRS
Start Date:12/2008
Abstract:
Travel grants for NSFC/RGC JRS


Project Title:Nano Mechanics of Individual Collagen Fibrils of the Human Intervertebral Discs Using Optical Trap Technique
Investigator(s):Lu WW, Ngan AHW, Luk KDK, Tang B
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:12/2008
Abstract:
1. Quantify the mechanical properties of individual collagen fibrils of human or animal intervertebral discs or related tissues more accurately through tensile tests using the Optical Tweezers (OT). 2. Investigate the change in the mechanics and structure-property relationship of the collagen fibrils or tissues with reference to the degenerated tissues.


Project Title:Guanidinylated polyethylenimine (PEI) as nano nonviral vector to transfer human umbilical cord mesenchymal stem cells (UC-hMSC) with BMP2 gene for bone formation
Investigator(s):Lu WW, Li Z, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2009
Abstract:
In Hong Kong, the number of osteoporotic fracture cases is ever increasing and it was ranked among the top-five conditions causing disability, prolonging the hospital stay of such patients [1]. According to a report from Hong Kong Hospital Authority, the projected cost for treating osteoporotic fracture cases will be 3-times higher by 2010 that in 1995 [2]. This being the scenario, the conventional fracture fixation techniques currently in practice for treating osteoporotic bone fractures are also proving unsatisfactory owing to the fragility and extremely low bone volume at the osteoporotic fracture sites. It is known that the bone morphogenetic proteins’ (BMPs) activity is essential for the initiation of fracture healing [3]. However, the key issue is that direct therapy with BMPs is limited by rapid protein inactivation, short-term bioavailability, requirement of large doses and high cost [4]. This calls for the support of gene therapy as a means to prolong the delivery of BMPs by in vivo synthesis. Success in gene therapy is largely dependent on the development of a safe and efficient vector (or vehicle) for gene transfer [5]. Molecular biologists have exploited viruses as vectors to efficiently transport the desired genetic materials inside the cells. Such viral vectors offer high efficiency in transducing the cells. Previously, we had developed adenoviral and adeno-associated viral vectors to transfer BMP2 and BMP4 gene for stimulating bone formation [6-9]. However, the immunogenicity and cytotoxicity of virus has limited the clinical usefulness of these methods. The high safety risk involved with using such viral vectors is evident from the first therapy-related fatality which was attributed to an inflammatory reaction to an adenovirus vector [10]. These side reactions provoked by the viral vectors, and other established and hypothetical safety concerns have prompted efforts to explore and improve non-viral delivery systems (vectors). Non-viral vectors are particularly suitable because of their higher genetic material carrying capacity while simple to use and easy to produce in large-scale. More importantly, unlike viral vectors, non-viral vectors lack specific immune response [5]. We have been investigating chitosan, its derivatives and other cationic polymers as non-viral vectors for gene transfer since year 2001 [11-14]. The unsatisfactory transfection efficiency resulting from the barriers encountered in the intracellular routing of plasmid DNA impose major hurdles to their clinical applications [15]. Polyethylenimine (PEI), a readily available synthetic polycation introduced for transfection a few years ago, has proven to be one of the most powerful and versatile members in the non-viral vector family due to its relatively high transfection efficiency and ease of functionalization [16]. It has been applied successfully for in vivo gene delivery to a variety of tissues [17-19]. However, the relatively high cytotoxicity of PEI has hindered its application in the clinical side. Some studies have tried coupling certain cell penetrating and pH-sensitive fusogenic peptides with PEI to enhance the transfection efficiency and reduce its cytotoxicity. These conjugated peptides may undergo degeneration by endogenous peptidases or may even induce immunogenicity. This has attracted scientists to chemically modify PEI by key moieties that are safer [20-22]. The high cytotoxic level of PEI is due to the accumulation of high charge density during protonation of the amino groups [23,24]. Guanidinium moieties, on the other hand, are known to play a key role in cellular uptake because of their highly basic nature and the formation of a specific bidentate hydrogen-bonding with the cell membrane [25]. Thus we arrived at the hypothesis that transforming the amino groups by guanidinium moiety of arginine residues will reduce the cytotoxicity of PEI while preserving and possibly further enhancing its already high transfection efficiency. We have confirmed the validity and viability of this hypothesis through pilot studies. human umbilical cord mesenchymal stem cells (UC-hMSC) is an important category of adult stem cell, which could be induced to differentiate into osteogenic, chondrogenic and adipogenic lineages under specific conditions. Compared with other source of mesenchymal stem cell, it has several advantages; firstly it has a wider source, the cells could be isolated from the mesenchymal tissue of umbilical cord during the birth and isolation process is easy to manipulate. Secondly, UC-hMSC has short doubling time (24 hours), proliferating fast in vitro culture, enabling the obtaining of large quantity of cells in relative short in vitro culture time. Thirdly UC-hMSC has immune modulating functions; immune response of allogenic UC-hMSC transplantation is very low compared with bone marrow derived mesenchyam stem cells (BMSC). The last and most important advantage is that UC-hMSC have more potential to differentiate into osteogenic lineage under specific conditions (such as BMP2 stimulation) [26], suggesting UC-hMSC is suitable for transplantation and enhancing bone formation. In this proposed study, we will optimize the guanidinylated polyethylenimine (G-PEI) further by changing the molecular weight of PEI and the substitution degree of guanidinium group, and apply this novel non-viral vector of optimized formula to transfect UC-hMSC with BMP2 gene to evaluate its efficacy in vitro. Objectives 1. Optimize the guanidinylated polyethylenimine (G-PEI) by altering (i) the molecular weight of PEI (800, 1200 and 25000Da) and (ii) the substitution degree of guanidinium group to construct a novel non-viral vector with improved transfection efficiency and reduced cytotoxicity. 2. Apply the G-PEI of optimized formula to transfect UC-hMSC with BMP2 gene and evaluate its efficacy in-vitro.


Project Title:Strontium-incorporated Coral as a Novel Biomaterial for Osteoporotic bone filler
Investigator(s):Lu WW, Pan H, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:06/2009
Completion Date:06/2010
Abstract:
Natural coral has been studied as a bone substitute experimentally for over 20 years. It also has been used for more than 10 years as a filling for defects or as only grafts in orthopedics and traumatology, in cranio facial, in dental and in neurosurgery. Bone substitutes also might be used to replace harvested autogenous bone at the donor site, if necessary. The prerequisite for reharvesting is that the bone substitute should resorb and be replaced by new bone tissue over time. Natural coral could be such a material as it is both biocompatible and osteoconductive and its mechanical properties are comparable to those of cancellous bone. The coral species most often used have pores measuring between 100 and 800 µm, allowing the ingrowth of fibrovascular and bone tissue. After implantation of natural coral, its calcium carbonate framework resorbs over time, meanwhile the new bone is formed. Strontium plays important role in the treatment of osteoporosis. It was reported that strontium can enhance preosteoblast differentiation, inhibit osteoclast differentiation, and therefore reduce osteoclast function. In our research team, we have successfully developed strontium-reinforced hydroxyapatite and related bone cement, showing excellent bioactivity and biocompatibility. The aim of this project is therefore to (1) prepare novel biomimetic strontium-incorporated hydroxyapatite from the source of coral by hydrothermal synthesis (2) prepare biomimetic coral scaffold coated by strontium-hydroxyapatite via hydrothermal treatment (3) file patent and seek industrial support for large scale fabrication Detailed aim of this work: 1) To select suitable coral for bone tissue engineering (support from Shanghai Ocean University has been confirmed, see attached letter) 2) To prepare biomimetic strontium-incorporated apatite for bone filler by hydrothermal synthesis from the source of natural coral 3) To coat natural coral by strontium-hydroxyapatite via hydrothermal treatment for bone replacement 4) To evaluate biocompatibility of this synthetic biomimetic product in vitro 5) Industrial support for large scale fabrication (support from Mr. Wong, Kyorin Group China Ltd, see attached letter) Key points: 1) There are hundreds of coral can be found in sea, the suitable candidate for biological application is the first step. Therefore, characterization of the source is important. 2) If apatite-like material can be formed by hydrothermal treatment, the control of pH are particularly important: high pH leads to the formation of apatite, the limitation of the pH has therefore to be determined. 3) Since phosphate is the requirement to form apatite, the addition of different amount of phosphate has to be studied due to the phase purity depends on Ca/P ratio. 4) If calcium can be easily substituted by strontium, the substitution limit and different substitution degree have to be studied and understood. Such different strontium-substituted apatite may act as the source of novel biomaterial. 5) The degradation rate of coral can be controlled by the surface coating of strontium-incorporated apatite, so the coating thickness needs to be studied. 6) Mechanical property of coral coated by strontium-substituted apatite has to be investigated. Problems: It seems there is no significant technical problem to prepare biomimetic strontium-incorporated apatite from natural coral and surface treatment of natural coral by hydrothermal treatment due to strong background in previous synthesis of strontium-related products in our research group. However, the problem may come from the source of coral, since collecting live coral is illegal, so we will collaborate with Shanghai Ocean University to resolve this problem (actually the collecting dead coral is on going).


Project Title:World Congress 2009 on Medical Physics and Biomedical Engineering (WC2009) Strontium phosphate chloride nanowire reinforced bioactive bone cement for Orthopaedic applications
Investigator(s):Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:09/2009
Completion Date:09/2009
Abstract:
N/A


Project Title:Role of strontium-calcium silicate - a novel biomaterial in the regeneration of osteoporotic bone - an in-vivo/ vitro study
Investigator(s):Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Travel Grants for NSFC/RGC JRS
Start Date:12/2009
Abstract:
Travel grants for NSFC/RGC JRS


Project Title:Optimization and commercialization of strontium containing bioactive bone cement for various orthopaedic applications
Investigator(s):Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Innovation and Technology Fund Internship Programme
Start Date:12/2009
Abstract:
(1) Optimize and commercialize three new brands of Sr-HA bone cement to fulfill the application in three major areas: (i) Osteoporotic spinal fracture (Sr-HA Spine), (ii) Total hip replacement implant augmentation (Sr-HA Hip) and (iii) Bone tumor, or bone cavity filler (Sr-HA Filler). (2) Provide a complete mechancial and biocompatibility profile of the modified Sr-HA cement formulation as per the ISO 5883 and ISO 10993 standards. (3) Carry out 30-35 cases of clinical trials on bioactive bone cement as a total hip replacement fixation agent. (4) Filing the standards for application to local or international medical device licensing organizations for marketing. The key issue of this study is to optimize present Sr-HA bioactive bone cement by modifying its composition to fulfill a wied variety of orthopaedic application requirements while preserving its desired properties of bioactivity and osteointegration. Ultimately, we aim at replacing the current non-bioactive PMMA bone cement with bioactive bone cements suitable for the particular needs which can significantly reduce clincial complications and improve the quality of life among the patients.


List of Research Outputs

Aladin K.D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nanostructure of Collagen Fibrils in Human Nucleus Pulposus and Its Correlation with Macroscale Tissue Mechanics, J. Ortho. Res.. 2009, 28: 497-502.
Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nano-structure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics, Journal of Orthopedic Research. 2010, 28: 497-502.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, Orthopaedic Research Society. 2010.
Chan K.Y., Leung Y.L., Tam V., Lu W.W., Sze K.Y. and Cheung K.M.C., Production of a natural disc scaffold for intervertebral disc tissue engineering, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Cheung K.M.C., Yeung K.W.K., Lu W.W. and Chung C.Y., Device for correcting spinal deformities, Japan patent no. 4423197, granted on December 11, 2009, 2009.
Cheung K.M.C., Yeung K.W.K., Lu W.W., Poon T.L. and Chow S.P., Fixation device, US Regular patent no. 7,695,471, granted on April 13, 2010, 2010.
Deng L.I.A.N.-.F.U., Wen C. and Lu W.W., Orthopaedic Research in China: Prospective Review, 我国骨科基础科学研究的现状与未来发展方向, Orthopedic Journal of China . 中国矫形外科杂志, 2009.
Duan B., Wang M., Li Z. and Lu W.W., Bone morphogenetic protein incorporated nanocomposite scaffolds and induction of osteogenic differentiation of mesenchymal stem cells, Proceedings of the Tissue Engineering and Regenerative Medicine International Society – EU Meeting -2010. Galway, Ireland, Paper #459.
Duan B., Wang M., Li Z. and Lu W.W., Ca-P/PHBV nanocomposite scaffolds modified by gelatin and heparin and their biological evaluation, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 94.
Duan B., Wang M., Li Z. and Lu W.W., Cytocompatibility and osteoconductivity of three-dimensional selective laser sintered nanocomposite scaffolds, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 95.
Duan B., Wang M., Li Z. and Lu W.W., In Vitro Biological assessment of nanocomposite scaffolds for bone tissue engineering, Proceedings of WACBE World Congress on Bioengineering 2009. Hong Kong, 20.
Duan B., Wang M., Li Z. and Lu W.W., Surface modification of selective laser sintered nanocomposite scaffolds and osteogenic differentiation of mesenchymal stem cells on the scaffolds, Transactions of the Society For Biomaterials 2010 Annual Meeting. Seattle, WA, USA, 2010, Paper #421.
Hu Y., Wong Y.L., Lu W.W. and Kawchuk G.N., Creation of an asymmetrical gradient of back muscle activity and spinal stiffness during asymmetrical hip extension., Clin Biomech. 2009, 24: 799-806.
Kuang G., Yau W.P., Lu W.W. and Chiu P.K.Y., Methods in enhancing osteointegration of soft tissue grafts within the bone tunnels in anterior cruciate ligament reconstruction, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S125.
Kuang G.M., Yau W.P., Lu W.W. and Chiu P.K.Y., Methods in Enhancing Osteointegration of Soft Tissue Grafts within the Bone Tunnels in Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Kuang G.M., Yau W.P. and Lu W.W., Osteointegration of soft tissue grafts within the bone tunnels in Anterior Cruciate Ligament reconstruction can be enhanced, Knee Surgery, Sports Traumatology, Arthroscopy . 2009, E publication.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Pan H., Li Z., Chen Y., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Fatty acid affects the nucleation of strontium-substituted biominerals, Ceram Int. 2010, 36(2): 683-688.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Lam S.K.L., Luk K.D.K. and Lu W.W., Biomechanics of the intervertebral disc allograft transplantation , 2010.
Lam S.K.L., Luk K.D.K., Ruan D.K., Ding Y. and Lu W.W., In-vivo kinematics of the IVD allograft transplantation, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lam S.K.L., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., In-vivo kinematics of the intervertebral disc allograft transplantation, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Lam S.K.L., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., The effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Lam S.K.L., Luk K.D.K., Xiao J., Ruan D.K., Ding Y. and Lu W.W., The kinematics of the mal-placement of the intervertebral disc allograft transplant on the segmental mobility, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lam W.M.R., Pan H., Li Z., Yang C., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Strontium-substituted calcium phosphates prepared by hydrothermal method under linoleic acid-ethanol solution, CERAMICS INTERNATIONAL. 2010, 36(2): 683-688.
Leung H.B., Cheung K.M.C., Lu W.W., Tseng H. and Luk K.D.K., The effect of insertion timing on pullout strength of pedicle screws from polymethylmethacrylate bone cement, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Canada patent no. 2,395,910, granted on September 22, 2009, 2009.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Hong Kong Standard patent no. HK1046378, granted on July 17, 2009, 2009.
Li Z., Pan H., Luk K.D.K. and Lu W.W., Bone Mechanical Properties Determination from Macro- to Nanoscopic Scale, The 3rd China International Congress and Exibition of Orthopaedics. 2009.
Li Z., Luk K.D.K. and Lu W.W., Strontium Containing Bioactive Bone Cement for Orthopaedic Applications, In: S. C. Tjong, Advances in Biomedical Sciences and Engineering. Bentham, 2009, 69-81.
Li Z., Lu W.W., Deng L., Chiu P.K.Y., Fang D., Lam R.W.M., Leong J.C.Y. and Luk K.D.K., The morphology and lattice structure of bone crystal after strontium treatment in goats, Journal Of Bone And Mineral Research. 2010, 28: 25-34.
Liang G.L., Yang Z.M., Zhang R.J., Li L.H., Fan Y.J., Kuang Y., Gao Y., Wang T., Lu W.W. and Xu B., Supramolecular Hydrogel of a D-Amino Acid Dipeptide for Controlled Drug Release in Vivo , LANGMUIR. 2009, 25(15): 8419-8422.
Lu W.W., Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T. and Luk K.D.K., Correlation between the nano-structure and macro-mechanics of human nucleus pulposus, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lu W.W., Tang W.K., Yeung K.W.K. and Luk K.D.K., In vitro kinematics study of human lumbar facet joints, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Luk K.D.K., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Lam S.K.L., Effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Luk K.D.K., Lu W.W., Zhou G., Peng S.L., Wang T. and Li Z., New Investigator Recognition Award., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010. 2010.
Ma W., Ng F.Y., Chiu P.K.Y., Li Z. and Lu W.W., The effects of acetabular component position and hip ball diameter on posterior stability after total hip replacement, Chinese Journal Of Joint Surgery. 2009, 3: 624-629.
Ni G., Lin J.H., Chiu P.K.Y., Li Z. and Lu W.W., Effect of strontium-containing hydroxyapatite bone cement on bone remodeling following hip replacement, Journal Of Material Science,. Materials In Medicine. 2010, 21: 377-384.
Pan H., Lam W.M.R., Zhao X., Li Z., Lu W.W., Wang D., Huang W., Lin K. and Chang J., Borosilicate for tissue engineering, World Association for Chinese Biomedical Engineers (WACBE). 2009.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Peng S.L., Liu X.W., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., Effect of different timing of strontium treatment on bone remodeling in ovariectomized rats, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Tang W.K., Luk K.D.K., Yeung K.W.K. and Lu W.W., In vivo kinematics of the lumbar facet joints for the development of a facet fixator, 2009.
Tang W.K., Yeung K.W.K., Lu W.W. and Luk K.D.K., In-vitro kinematics study of human lumbar facet joints, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Tong H.W., Wang M. and Lu W.W., Cell behaviour on electrospun biodegradable fibrous membranes: Effects of fiber diameter and fiber alignment, Proceedings of Materials Science & Technology 2009 Conference & Exhibition. Pittsburg, USA, 2009, CD-ROM: 1pp.
Tong H.W., Wang M., Li Z. and Lu W.W., Effects of the alignment, diameter and surface morphology of electrospun fibers on the behaviour of osteoblastic cells, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 107.
Tong H.W., Wang M., Li Z., Lam W.M.R. and Lu W.W., Electrospinning of surface-porous fibers and In vitro biological study of these fibers, Proceedings of WACBE World Congress on Bioengineering 2009. Hong Kong, 38.
Tong H.W., Wang M. and Lu W.W., In Vitro Biological evaluation of fibrous PHBV polymer and CHA/PHBV nanocomposite scaffolds developed for tissue engineering applications, Proceedings of the 22nd International Symposium on Ceramics in Medicine (Bioceramics 22). Daegu, Korea, 2009, 453-456.
Tong H.W., Wang M., Li Z. and Lu W.W., In vitro biological assessment of electrospun composite fibers containing hydroxyapatite nanoparticles, Proceedings of the 2nd International Symposium on Surface and Interface of Biomaterials (ISSIB-II). Hong Kong, 2010, 108.
Tong H.W., Wang M. and Lu W.W., In vitro biological evaluation of fibrous PHBV polymer and CHA/PHBV nanocomposite scaffolds developed for tissue engineering applications, The 22nd International Symposium on Ceramics in Medicine (Bioceramics 22), Daegu, South Korea. 2009.
Tong H.W., Wang M., Li Z. and Lu W.W., The influence of fiber diameter, morphology and alignment on osteoblastic cell behaviour, Proceedings of the National Biomaterials Congress. Chengdu, China, 2010, CD-ROM: 1pp.
Tong H.W., Li Z., Wang M. and Lu W.W., 电纺纤维直径、表面形貌及排列状态对细胞的影响, 全国生物材料大会, 2010.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.
Yeung K.W.K., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Tu H., Chu P.K. and Liu X.Y., Alterations of Surface Mechanics of Nickel Titanium Shape Memory Alloys after Plasma Surface Treatment. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010).. 2010, 110-111.
Yeung K.W.K., Lai D.W.T., Lai D.W.T., Lai D.W.T., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Wu S.L., Tu H., Chu P.K. and Chung C.Y., Biomechanical Analysis of Plasma Treated NiTi Materials for Spinal Deformity Correction., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May 2010). 2010, 24-25.
Yeung K.W.K., Lam W.N., Natarajan D., Wu S.L., Hu T., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Nickel Ion Level in Scoliotic Patients Implanted with Nitrogen Plasma Surface Modified Nickel-Titanium Superelastic Spinal Implant., 3rd IEEE International NanoElectronics Conference (INEC), Hong Kong, 3-8 January 2010. Oral presentation.. 2010, Paper BC410, p. 134.
Yeung K.W.K., Lam W.N., Wu S.L., Tu H., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Post-Operative Monitoring of Nickel Ion Level in Scoliotic Patients Operated with Novel Nickel-Titanium Superelastic Spinal Implant. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20 May 2010). 2010, 22-23.
Yeung K.W.K., Wu S.L., Tu H., Chu P.K., Liu X.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Suppression of Surface Nickel Concentration of Nickel Titanium Shape Memory Alloys by Plasma Surface Treatment., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 112-113.
Zhao X., Li Z., Liu W.G., Pan H., Lam R.W.M., Luk K.D.K. and Lu W.W., Characterization and evaluation of octa-arginine conjugated chitosan as non-biral vector for gene transfer, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Zheng Z.M., Yu B.S., Chen H., Aladin Kaderbatcha D.M., Zhang K.B., Zhang J.F., Liu H., Luk K.D.K. and Lu W.W., 251. Effect of iliac screw insertion depth on the stability and strength of lumbo-iliac fixation constructs. An anatomical and biomechanical study, Spine. 2009, 34(16): E565-E572.


Researcher : Lui SC

List of Research Outputs

Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of PEEK Bioactivity by Plasma Surface Modification., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug-3Sep 2009. Poster presentation.. 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of Osteoblast Attachment and Growth on PEEK by Water and Ammonia Plasma Surface Treatment. (6-9 Mar 2010), 56th Annual Meeting of the Orthopaedic Research Society, New Orleans. 2010, Paper 1193.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of osteoblast attachment and growth on PEEK by water and ammonia plasma surface treatment, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Lui S.C., Yeung K.W.K., Chu P.K., Kwok T.K., Pan H., Luk K.D.K. and Cheung K.M.C., Plasma-modified polyetheretherketone for orthopaedic applications: bulk and surface mechanical characterisations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Lui S.C., Cheung K.M.C. and Yeung K.W.K., Surface bioactivity enhancement of polyetheretherketone by plasma immersion ion implantation, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., The Effect of Plasma Technology in Bioactivity Enhancement of PEEK. , 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep2009. Poster presentation.. 2009.
Yeung K.W.K., Lui S.C., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of Cell Adhesion and Proliferation on Polyetheretherketone by Using Plasma Surface Modification., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24April2010.. 2010, 864.


Researcher : Lui SC

List of Research Outputs

Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of PEEK Bioactivity by Plasma Surface Modification., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug-3Sep 2009. Poster presentation.. 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of Osteoblast Attachment and Growth on PEEK by Water and Ammonia Plasma Surface Treatment. (6-9 Mar 2010), 56th Annual Meeting of the Orthopaedic Research Society, New Orleans. 2010, Paper 1193.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of osteoblast attachment and growth on PEEK by water and ammonia plasma surface treatment, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Lui S.C., Yeung K.W.K., Chu P.K., Kwok T.K., Pan H., Luk K.D.K. and Cheung K.M.C., Plasma-modified polyetheretherketone for orthopaedic applications: bulk and surface mechanical characterisations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Lui S.C., Cheung K.M.C. and Yeung K.W.K., Surface bioactivity enhancement of polyetheretherketone by plasma immersion ion implantation, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., The Effect of Plasma Technology in Bioactivity Enhancement of PEEK. , 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep2009. Poster presentation.. 2009.
Yeung K.W.K., Lui S.C., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of Cell Adhesion and Proliferation on Polyetheretherketone by Using Plasma Surface Modification., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24April2010.. 2010, 864.


Researcher : Luk KDK

Project Title:Provision of individualized biomechanical and electrophysiological service for occupational low back disorder patients
Investigator(s):Luk KDK, Lu WW, Hu Y
Department:Orthopaedics and Traumatology
Source(s) of Funding:S.K. Yee Medical Foundation - General Award
Start Date:12/2003
Abstract:
To assess the low back muscular function and biomechanical problems of low back disorder patients to identify, evaluate and quantify potential of factors of OLBD; to study the drawbacks and benefits of conventional treatments; to introduce an individualized biomechanical and electrophysiological assessment service to identify specific OLBD problems in individual patients, and provide guidelines both for an optimal rehabilitation treatment and for a future workplace regimen to prevent recurrence.


Project Title:Biomedical Engineering
Investigator(s):Luk KDK, Cheung PYS
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding for Strategic Research Theme
Start Date:06/2008
Completion Date:05/2011
Abstract:
n/a


Project Title:A functional MRI study of the chronically compressed spinal cord in HUMANS.
Investigator(s):Luk KDK, Hu Y, Wu EX
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2009
Abstract:
(1) To develop an in-vivo MRI protocol using anatomical, perfusion and BOLD functional imaging to study the anatomic and hemodynamic changes of the spinal cord in CM; (2) To develop a MRI protocol using DTI to monitor the ultrastructural changes (demyelination and axonal degeneration) in the spinal cord in CM; (3) To assess the efficacy of such MRI measurements by correlating with the clinical presentation, electrophysiological and radiographic measurements.


Project Title:Development of a facet joint fixator
Investigator(s):Luk KDK, Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2009
Completion Date:06/2010
Abstract:
The objectives are: • To investigate the kinematics of the facet joints at L4/5 in vitro using motion analysis system, and • To develop a novel facet joint fixator so as to limit the degree of motion of the L4/5 facet joint. Facet joints (FJ), working with the intervertebral disc as three-joint complex, share loadings, provide spinal motion and maintain spinal integrity [1]. Osteoarthritis of the facet joints, may create central and/or lateral stenosis and hence back pain [2]. Besides, any anatomical defects in the joints may lead to spinal instabilities [3]. Clinical treatments on facet joints include joint injection, artificial joint replacement and spinal fusion. Facet joint injection has been presented as the therapeutic option for low back pain to act as nerve blocks of the joints. Literatures show it provides a short-term pain relief. However, since the drug is only injected at the facet joints, it cannot alleviate back pain primarily from the disc, ligaments or bone. More importantly, the effect is not long-lasting and no help with the instabilities introduced by impaired joints [4, 5]. Artificial facet joint replacement acts as an alternative to spinal fusion. It replaces painful facet joints and provides dynamic stabilization for the functional segment as an adjunct to disc arthroplasty or facetectomy performed for neural decompression. Examples are Total Facet Arthroplasty System (TFAS) and the Total Posterior Element Replacement System (TOPS). By motion preservation, the prostheses may prevent adjacent-segment disease by maintaining normal load transmission and flexibility [6]. In vitro biomechanical studies have proven that they restore the range of motion comparable to that of an intact segment [7, 8], and short-term clinical study shows excellent short-term functional and radiographic outcomes in treating degenerative spondylolisthesis [9]. However, previous in vitro studies demonstrated the contribution of the joint capsule to total segment stiffness in flexion [10, 11]. Joint replacement may relieve pain, but cannot fully restore joint functionality due to lack of capsular ligaments. Therefore, the first criteria is to introduce limitations to the spinal movement in order to stabilize the facet joints and hence the spinal segment. With a long history, spinal fusion is a standard surgical procedure indicated for a wide range of spinal disorders [12]. It aims to establish structural balance through fusing the two adjacent vertebrae by bone grafts. Long-term clinical studies reveal promising results in pain relief and maintenance of stability with high fusion rates. Many studies have illustrated the problems of adjacent segment degeneration associated with spinal fusion due to altered stress phenomenon and hyper-mobility at adjacent levels [13]. However, whether adjacent degeneration is attributed to spinal fusion or natural history of underlying degenerative disease remains questionable. Even when equipped with dynamic stabilization device, progressive disc degeneration was observed at adjacent levels [14]. Since the range of motion in a spinal segment is relatively small comparing to hip or knee joint, the effect of minimizing the motion at one segment would not be so significant. In contrast, it is believed that establishing restrains on the range of motion is always essential to restore the stability of a destabilized segment. In general, spinal fusion consists of posterolateral fusion, interbody fusion and interfacet fusion. Studies show, even when solid posterolateral fusion is achieved, persisting micromotion of the fused segments sensitizes the nociceptors within the disc annulus, which may produce continued low back pain [15]. Interbody fusion offers some advantages over posterolateral fusion that the interbody grafts are compressed by a greater proportion of spinal loads and can better restore coronal and sagittal balance [16]. Besides, in both posterolateral fusion and interbody fusion, bony fusion is commonly assisted with bone screws applying through the pedicles attaching to a metal rod or plate bilaterally. The superior initial immobilization favors the process of arthrodesis [17]. However, it is important to be aware of the risks induced by transpedicular instrumentation. The bulky device involves lengthy surgery and significant blood loss. The associated complications include neurological injury, deep tissue infection and failure of instrumentation. Furthermore, coupling failure between rods and nuts, and screw breakage, may inevitably lead to loss of stabilization offered by the implants. [18, 19]. Current interfacet fusion techniques include transfacet screw fixation and translaminar facet screw fixation [17]. Facet fixation involves fewer surgical procedures compared to pedicle screw-rod system, resulting in less dorsal destruction of bony elements, lower incidence of neurological damage and lower complication rates with shorter operation time. The effectiveness is supported by biomechanical studies and clinical results with very stiff and strong fixation [20-23]. Moreover, facet lag screws and a novel transfacet fixation screw that exert compression at the joint surfaces have been found to increase the biomechanical stability of the bone-implant interface and provide comparable stiffness to pedicle screw systems [24, 25]. On the other hand, the problem of current technique is that the placement of facet screws penetrating the facet joint surfaces requires significant precision in terms of screw position and screw length, which is technically demanding. The high requirement in surgical technique, as well as possible screw loosening and breakage, would certainly hinder the use of facet joint fixation. Therefore, improvement of the implant specially designed for facet joint fixation is needed. A non-screw type device with simple surgical procedures would be desired to encourage the use of facet joint fixation. Besides, a new clamp-like fixator has been proposed in our pilot studies to provide restraints on the joints and manifested to be effective in reducing the spinal motion. It is believed a fixator, exhibiting the ability to apply clamping forces across the facet joints without the need of screw insertion, has the potential to become a promising technique in facet joint fixation. To facilitate the design of new fixator, the kinematics of the facet joints should be carefully investigated. This would be essential to design an appropriate implant that can abolish the segment motion and enhance the fusion process. It is hypothesized a fixator applying clamping forces across the facet joint surfaces can significantly reduce the overall spinal motion in all anatomical planes.


Project Title:A mesenchymal stem cell-based approach to rescue intervertebral disc allograft from post-transplantation degeneration
Investigator(s):Luk KDK, Cheung KMC, Leung YL, Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2010
Abstract:
1) Test if MSC-implanted disc allografts show reduced degeneration after transplantation in an ovine model; 2) Test if endplate healing can affect the regenerative potential and fate of the implanted MSC; 3) Test if MSC implantation may improve the biomechanics/kinematics of the transplanted spinal segment.


Project Title:Haemodynamic Dysfunction in Cervical Spine Chronic Compressive Injuries: An Experimental Study
Investigator(s):Luk KDK, Hu Y, Wen C
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:03/2010
Abstract:
(a) Key issues Cervical myelopathy (CM) is one of the most common spinal cord disorders affecting the elderly [1, 2]. It is caused by the chronic compression of the spinal cord due to spinal stenosis as a result of spondylosis, disc degeneration, ossification of the posterior longitudinal ligament, congenital narrow canal etc, ultimately leading to the loss of motor and sensory function of the spinal cord. With a rapidly growing aging population in Hong Kong, CM is anticipated to cause a heavy burden on the region’s public health services and its socioeconomic system. Yet the pathophysiology of CM is uncertain due to its insidious onset, variety of clinical signs and symptoms and lack of any pathognomonic signs, thus posing a big challenge on clinical diagnosis and referral to surgery. So far, the putative mechanism of CM is thought to be ischemia associated with static or dynamic mechanical compression of the spinal cord [3]. Ishemia may result from direct compression of the large vessels such as the anterior spinal artery, or overall reduction of flow in the pial plexuses and the penetrating small arteries that supply the cord, or impairment of venous flow leading to venous congestion [4]. Histological examination of autopsy specimens have shown a loss of motoneurons and vacuolar degeneration in the gray matter in the late stage of CM [5, 6]. As motoneurons and oligodendrocytes are susceptible to ischemia, ischemia may account for demyelization and motor deficit in CM patients. However, such a simple pathoanatomic concept that a narrowed spinal canal causes compression of the enclosed cord, leading to local tissue ischemia, injury and neurological impairment, fails to explain the entire spectrum of clinical findings observed in CM patients. A growing body of evidence, from our studies and those of others, show that the compression ratio of spinal cord does not necessarily correlate with the neurological functional deficit or recovery. Moreover, cell death following chronic compression of the spinal cord is apoptosis related rather than due to necrosis. All these clues indicate that there is a “secondary pathogenesis” beyond the primary chronic compression injuries and the associated ischemia. Recently, the investigators have developed an experimental rat model to mimic cervical spinal cord chronic compressive injuries for studying the natural course of CM’s pathophysiology. In rats with severe neurological deficit, the cell number in gray matter and myelin in white matter are significantly lower with more blood vessels than in rats with moderate neurological deficit. These preliminary findings suggest that the vascular response to initial mechanical compression and associated ischemia may be involved in “secondary pathophysiology”. The exact mechanism of vascular events during the natural course of chronic spinal cord compressive injuries remains to be elucidated. (b) Objectives (1) establish a protocol to in-vivo monitor the haemodynamic function of rat spinal cord using contrast enhanced micro-computed , and (2)apply it to evaluate haemodynamic dysfunction during the natural course of chronic spinal cord compressive injuries in our established rat model. Refeerence: 1.McCormick WE, Steinmetz MP, Benzel EC. Cervical spondylotic myelopathy: make the difficult diagnosis, then refer for surgery. Cleve Clin J Med 2003;70: 899-904. 2.Ichihara K, Taguchi T, Sakuramoto I, Kawano S, Kawai S. Mechanism of the spinal cord injury and the cervical spondylotic myelopathy: new approach based on the mechanical features of the spinal cord white and gray matter. J Neurosurg 2003;99: 278-285. 3.Baron EM, Young WF. Cervical Spondylotic Myelopathy: A Brief Review of its Pathophysiology, Clinical Course, and Diagnosis. Neurosurgery 2007;60. 4.Yoshizawa H. Presidential address: pathomechanism of myelopathy and radiculopathy from the viewpoint of blood flow and cerebrospinal fluid flow including a short historical review. Spine (Phila Pa 1976) 2002;27: 1255-63. 5.Zhang ZG, Yang JL, Chan SC, Luk KD, Hu Y. Time-frequency component analysis of somatosensory evoked potentials in rats. Biomed Eng Online 2009;8: 4. 6.Hu Y, Ding Y, Ruan D, Wong YW, Cheung KMC, Luk KDK. Prognostic Value of Somatosensory-Evoked Potentials in the Surgical Management of Cervical Spondylotic Myelopathy. Spine 2008;33: E305-E310. 7.Kim P, Haisa T, Kawamoto T, Kirino T, Wakai S. Delayed Myelopathy Induced by Chronic Compression in the Rat Spinal Cord. Ann Neurol 2004;55: 503-511. 8.Wen CY, Qin L, Lee KM, Chan KM. The use of brushite calcium phosphate cement for enhancement of bone-tendon integration in an anterior cruciate ligament reconstruction rabbit model. J Biomed Mater Res B Appl Biomater 2009;89B: 466-74. 9.Wen CY, Qin L, Lee KM, Chan KM. Peri-graft bone mass and connectivity as predictors for the strength of tendon-to-bone attachment after anterior cruciate ligament reconstruction. Bone 2009;45: 545-52. 10.Wen CY, Qin L, Lee KM, Wan-Nar Wong M, Chan KM. Influence of bone adaptation on tendon-to-bone healing in bone tunnel after anterior cruciate ligament reconstruction in a rabbit model. J Orthop Res 2009.


List of Research Outputs

Aladin K.D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nanostructure of Collagen Fibrils in Human Nucleus Pulposus and Its Correlation with Macroscale Tissue Mechanics, J. Ortho. Res.. 2009, 28: 497-502.
Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T., Luk K.D.K. and Lu W.W., Nano-structure of collagen fibrils in human nucleus pulposus and its correlation with macroscale tissue mechanics, Journal of Orthopedic Research. 2010, 28: 497-502.
Chan B.P., Cheng H.W., Chik T.K., Cheung K.M.C. and Luk K.D.K., Methods for Complex Tissue Engineering, Provisional Patent Application (filed on 15 June 2010) (Application No. 61/354,869). 2010.
Chan C.W., Lam S.K.L., Leung Y.L., Chan D., Luk K.D.K. and Cheung K.M.C., Minimizing cryopreservation-induced loss of disc cell activity for storage of whole intervertebral discs, European Cells and Materials . 2010, 19: 273-283.
Chang K.C., Samartzis D., Luk K.D.K. and Cheung K.M.C., Cervical spine disease in Asian populations, Hong Kong Medical Journal. 2010, 16: 69-70.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S.S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Cheung K.M.C., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Cheung K.M.C., Samartzis D., Natarajan D., Li J., Cheung W.Y., Lenke L.G. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in scoliosis correction with alternate level pedicle screw fixation, Journal of Bone and Joint Surgery. 2010, 92: 169-176.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation., 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.. 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, J Bone Joint Surg (Am). 2010, 92: 169-76.
Cheung K.M.C., Mok P.S., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl's nodes of the lumbar spine: prevalence, classification, associated risk factors and clinical relevance, 83rd Annual Meeting of the Japanese Orthopaedic Association, Tokyo, Japan, May 27-30, 2010.
Cheung K.M.C., Samartzis D., Karppinen J., Mok P.S., Ho D.W.H., Fong D.Y.T. and Luk K.D.K., “Skipped” level disc degeneration of the lumbar spine, a MRI study of 1989 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Cheung W.Y., Lenke L.G. and Luk K.D.K., Best Spine Paper Award. Prediction of scoliosis correction with thoracic segmental pedicle screw constructs using fulcrum bending radiograph., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., In-vivo demonstration of spontaneous vertebral derotation in thoracic adolescent idiopathic scoliosis with fulcrum bending radiograph, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Cheung W.Y., Lenke L.G. and Luk K.D.K., Prediction of scoliosis correction with thoracic segmental pedicle screw constructs using fulcrum bending radiographs, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Chik T.K., Luk K.D.K., Cheung K.M.C. and Chan B.P., Creation of a tissue interface between bone-like construct and soft tissue-like construct. , TERMIS 2nd World Congress, Seoul, Korea, Aug 31- 3 Sep 2009. S211: 802.
Choi M.Y., Chan C.C.Y., Chan D., Luk K.D.K., Cheah K.S.E. and Tanner J.A., Biochemical consequences of sedlin mutations that cause spondyloepiphyseal dysplasia tarda, Biochemical Journal. Great Britain, Biochemical Society, 2009, 423: 233-242.
Cui J., Wen C., Hu Y. and Luk K.D.K., Precise Diagnosis of Cervical Spondylotic Myelopathy based on Quantitative Fiber Tractography, The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Fok A., Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The prevalence, factors, and clinical profile associated with Tarlov cysts in Southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Fok A.W.M., Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The prevalence, factors, and clinical profile associated with tarlov cysts in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Fong D.Y.T., Lee C.F., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., A meta-analysis of the clinical effectiveness of school scoliosis screening, Spine. 2010, 35(10): 1061-1071.
Fong D.Y.T., Lee C.F., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., A meta-analysis of the clinical effectiveness of school scoliosis screening, Spine (Phila Pa 1976). 2010, 35(10): 1061-1071.
Guo J.J., Luk K.D.K., Karppinen J., Yang H.L. and Cheung K.M.C., Prevalence, distribution, and morphology of ossification of the ligamentum flavum, Spine. 2010, 35(1): 51-56.
Hu Y., Siu H.F., Mak N.F.J. and Luk K.D.K., Lumbar Muscle Electromyographic Dynamic Topography During Flexion-extension., J Electromyogr Kinesiol.. 2010, 20: 246-55.
Hu Y., Siu H.F., Mak J.N.F. and Luk K.D.K., 256. Lumbar muscle electromyographic dynamic topography during flexion-extension, Journal of Electromyography and Kinesiology. 2010, 20: 246-255.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Multiple Bioimaging Modalities Application for Chronic Compressive Spinal Cord of Rat. , the 54th Annual Meeting of the Orthopedic Research Society. New Orleans, Louisiana; 2010.
Hu Y., Siu H.F. and Luk K.D.K., Quantitative surface electromyography topography for low back pain assessment, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Hu Y., Liu H. and Luk K.D.K., Signal-to-Noise Ratio of Intraoperative Tibial Nerve Somatosensory-Evoked Potentials. , J Clin Neurophysiol.. 2010, 27: 30-3.
Hu Y., Liu H. and Luk K.D.K., Signal-to-noise ratio of intraoperative tibial nerve somatosensory-evoked potentials, Journal of Clinical Neurophysiology. 2010, 27(1): 30-33.
Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment. , The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK).. Aalborg Denmark., 2010.
Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment., The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK). 16-19 June, 2010, Aalborg Denmark. 2010.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Ultrastructural imaging of Cervical Spinal Cord with Induced Compressive Injuries in a Rat Model., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Kung A.W.C., Xiao S., Cherny S.S., Li H.Y., Gao Y., Tso G., Lau K., Luk K.D.K., Liu J.M., Cui B., Zhang M.J., Zhang Z.L., He J.W., Yue H., Xia W.B., Luo L.M., He S.L., Kiel D.P., Karasik D., Hsu Y.H., Cupples L.A., Demissie S., Styrkarsdottir U., Halldorsson B.V., Sigurdsson G., Thorsteinsdottir U., Stefansson K., Richards B., Zhai G., Soranzo N., Valdes A., Spector T.D. and Sham P.C., Association of JAG1 with Bone Mineral Density and Osteoporotic Fractures: A Genome-wide Association Study and Follow-up Replication Studies, American Journal of Human Genetics. 2010, 86 (2): 229-239.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Spine Chapter's Most Promising Spine Paper Award. Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Pan H., Li Z., Chen Y., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Fatty acid affects the nucleation of strontium-substituted biominerals, Ceram Int. 2010, 36(2): 683-688.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Lam S.K.L., Luk K.D.K. and Lu W.W., Biomechanics of the intervertebral disc allograft transplantation , 2010.
Lam S.K.L., Luk K.D.K., Ruan D.K., Ding Y. and Lu W.W., In-vivo kinematics of the IVD allograft transplantation, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lam S.K.L., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., In-vivo kinematics of the intervertebral disc allograft transplantation, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Lam S.K.L., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., The effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Lam S.K.L., Luk K.D.K., Xiao J., Ruan D.K., Ding Y. and Lu W.W., The kinematics of the mal-placement of the intervertebral disc allograft transplant on the segmental mobility, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lam W.M.R., Pan H., Li Z., Yang C., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Strontium-substituted calcium phosphates prepared by hydrothermal method under linoleic acid-ethanol solution, CERAMICS INTERNATIONAL. 2010, 36(2): 683-688.
Lee C.F., Fong D.Y.T., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Mak K.H., Yip P.S.F. and Luk K.D.K., A meta-analysis of the clinical effectiveness of school scoliosis screening, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lee C.F., Fong D.Y.T., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., Costs of School Scoliosis Screening: A Large, Population-Based Study, Spine (Phila Pa 1976). 2010, Epub ahead of print.
Leung H.B., Cheung K.M.C., Lu W.W., Tseng H. and Luk K.D.K., The effect of insertion timing on pullout strength of pedicle screws from polymethylmethacrylate bone cement, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Leung Y., Natarajan D., Samartzis D., Borkhuu B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis (AIS) using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Li J., Cheung K.M.C., Samartzis D., Zhu X., Li M. and Luk K.D.K., Key –Vertebral Screw Strategy (KVSS): an alternative surgical strategy for main thoracic curve correction in patients with adolescent idiopathic scoliosis, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Li J., Cheung K.M.C., Samartzis D., Zhu X.D., Li M. and Luk K.D.K., Key-Vertebral Screw Strategy (KVSS): an alternative surgical strategy for main thoracic curve correction in patients with adolescent idiopathic scoliosis, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009 . 2009.
Li T.H., Luk K.D.K., Hu Y. and Wu E.X., Ultrastructural imaging of the cervical spinal cord , 2010.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Canada patent no. 2,395,910, granted on September 22, 2009, 2009.
Li Y.W., Lu W.W., Cheung K.M.C., Luk K.D.K. and Leong J.C.Y., Bioactive and osteoporotic bone cement, Hong Kong Standard patent no. HK1046378, granted on July 17, 2009, 2009.
Li Z., Pan H., Luk K.D.K. and Lu W.W., Bone Mechanical Properties Determination from Macro- to Nanoscopic Scale, The 3rd China International Congress and Exibition of Orthopaedics. 2009.
Li Z., Luk K.D.K. and Lu W.W., Strontium Containing Bioactive Bone Cement for Orthopaedic Applications, In: S. C. Tjong, Advances in Biomedical Sciences and Engineering. Bentham, 2009, 69-81.
Li Z., Lu W.W., Deng L., Chiu P.K.Y., Fang D., Lam R.W.M., Leong J.C.Y. and Luk K.D.K., The morphology and lattice structure of bone crystal after strontium treatment in goats, Journal Of Bone And Mineral Research. 2010, 28: 25-34.
Liao H., Huang S., Cheung K.M.C., Chan D., Luk K.D.K. and Zhou G., Functional characterization of miRNA125b, miRNA140 and miRNA199a in chondrogenetic differentiation of mesenchymal stem cells, Cell Adhesion & Communication.. 2009, In press.
Loong C.H.N., Leung F., Lau T.W., Leung E., Chan Y.Y., Yee A., Ma L.F., Soong S.S., Bow C.H.Y., Yeung S.C., Luk K.D.K. and Kung A.W.C., Predictive Factors for Re-fracture in Chinese Population with Previous Osteoporotic Fractures, 11th Regional Osteoporosis Conference, Hong Kong. 2010.
Loong H.N.C., Chan Y.Y., Lau T.W., Leung F., Bow C.H.Y., Soong S.S., Ma L.F., Leung E., Yee A., Yeung S.C., Luk K.D.K. and Kung A.W.C., A Secondary Fracture Prevention Programme to Reduce Fractures, Hospital Admissions, and Mortality, Hospital Authority Convention 2010, Hong Kong. 2010.
Loong H.N.C., Chan Y.Y., Lau T.W., Leung F., Bow C.H.Y., Soong S.S., Ma L.F., Leung E., Yee A., Yeung S.C., Luk K.D.K. and Kung A.W.C., Evaluation of the Osteoporosis Secondary Fracture Prevention Program at Queen Mary Hospital: Successful Recruitment is Associated with a Lower Re-fracture Rate and Mortality Rate at One Year, Hospital Authority Convention 2010, Hong Kong. 2010.
Loong H.N.C., Chan Y.Y., Lau T.W., Leung F.K.L., Luk K.D.K. and Kung A.W.C., Evaluation of the Osteoporosis Secondary Fracture Prevention Program at Queen Mary Hospital: Successful Recruitment is associated with Lower Re-fracture and Mortality Rates at One and Five years Osteoporosis Society of Hong Kong, 11th Regional Osteoporosis Conference, Hong Kong, 15-16 May, 2010, 2010.
Lu W.W., Aladin Kaderbatcha D.M., Cheung K.M.C., Ngan A.H.W., Chan D., Leung Y.L., Lim C.T. and Luk K.D.K., Correlation between the nano-structure and macro-mechanics of human nucleus pulposus, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lu W.W., Tang W.K., Yeung K.W.K. and Luk K.D.K., In vitro kinematics study of human lumbar facet joints, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of PEEK Bioactivity by Plasma Surface Modification., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug-3Sep 2009. Poster presentation.. 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of Osteoblast Attachment and Growth on PEEK by Water and Ammonia Plasma Surface Treatment. (6-9 Mar 2010), 56th Annual Meeting of the Orthopaedic Research Society, New Orleans. 2010, Paper 1193.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of osteoblast attachment and growth on PEEK by water and ammonia plasma surface treatment, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Lui S.C., Yeung K.W.K., Chu P.K., Kwok T.K., Pan H., Luk K.D.K. and Cheung K.M.C., Plasma-modified polyetheretherketone for orthopaedic applications: bulk and surface mechanical characterisations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., The Effect of Plasma Technology in Bioactivity Enhancement of PEEK. , 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep2009. Poster presentation.. 2009.
Luk K.D.K., Wan T., Wong Y.W., Cheung K.M.C., Chan Y.K., Cheng A., Kwan M., Law K., Lee P.W.H. and Cheing G.L.Y., A multidisciplinary functional restoration program for patients with chronic back pain, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Luk K.D.K., Bone mechanical properties determination from macro- to nanoscopic scale, 3rd China International Congress of Orthopaedics, Beijing, PR China, August 6-9, 2009.
Luk K.D.K., Kamath V., Avadhani A. and Rajasekaran S., Cervical laminoplasty, Euoropean Spine Journal. 2010, 19: 347-348.
Luk K.D.K., Cervical radiculopathy: when to intervene surgically , AOSpine Global Interactive-College Course, Kuala Lumpur, Malaysia, October 26-30, 2009.
Luk K.D.K., Classifications of adolescent idiopathic scoliosis – how good are they? , Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Luk K.D.K., Coronal-sagittal-axial deformity coupling in thoracic AIS, 2nd China SRS International Meeting, Nanjing, PR China, June 4-6, 2010.
Luk K.D.K., Vidyadhara S., Lu D.S., Wong Y.W., Cheung W.Y. and Cheung K.M.C., Coupling between sagittal and frontal plane deformity correction in idiopathic thoracic scoliosis and its relationship with post-operative sagittal alignment, Spine. 2010, 35(11): 1158-1164.
Luk K.D.K., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Lam S.K.L., Effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Luk K.D.K., In-vivo kinematics of the IVD allograft transplantation, 3rd Asia Pacific Cervical Spine Society 2009, Beijing, PR China, November 7-8, 2009.
Luk K.D.K., Intervertebral disc allograft transplantation a biological approach to disc replacement. , 5th International Symposium of Chosun University Hospital: Spinal Disorders, Gwangju, Korea, November 14, 2009.
Luk K.D.K., Intervertebral disc transplantation – from laboratory to clinical trial., Frontiers in Biomedical Research HKU 2009, Hong Kong, December 4, 2009.
Luk K.D.K., Limitations of the MRC trials on TB spine and late paraplegia from myelomalacia , 3rd China International Congress of Orthopaedics, Beijing, PR China, August 6-9, 2009.
Luk K.D.K., Management of post-infection kyphosis , HA Commissioned Training in Adult Spinal Deformity, Hong Kong, November 26-27, 2009.
Luk K.D.K., Lu W.W., Zhou G., Peng S.L., Wang T. and Li Z., New Investigator Recognition Award., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010. 2010.
Luk K.D.K., Role of motion preservation vs fusion , AOSpine Global Interactive-College Course, Kuala Lumpur, Malaysia, October 26-30, 2009.
Luk K.D.K., Strontium fortified calcium nano- and microparticle compositions and methods of making and using thereof, PCT Patent No.: 2008001211 filed 27 January 2010. 2010.
Luk K.D.K., Vertical instability in spondylolisthesis, management principles, 4th International Congress of Chinese Orthopaedic Association, Xiamen, PR China, November 19-22, 2009.
Ming D., Bai Y., Liu X., Qi H., Cheng L., Wan B., Hu Y., Wong Y.W., Luk K.D.K. and Leong J.C.Y., A gait stability investigation into FES-assisted paraplegic walking based on the walker tipping index. , J Neural Eng. 2009, 6: 6600-7.
Ming D., Hu Y., Zhang G.J., Xue Z.J., Li L.Y., Liu X.Y., Bai Y.R., Cheng L.L., Qi H.Z., Wan B.K. and Luk K.D.K., Measurement of upper extremity joint moments in walker-assisted gait, IET Science, Measurement & Technology. 2009, 3(5): 343-353.
Ming D., Hu Y., Wong Y.W., Wan B.K., Luk K.D.K. and Leong J.C.Y., Risk-tendency graph (RTG): a new gait-analysis technique for monitoring FES-assisted paraplegic walking stability., Med Sci Monit. . 2009, 15: 105-12.
Mok F., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., 2010 ISSLS Prize Winner: Prevalence, determinants, and association of Schmorl's nodes of the lumbar spine with disc degeneration: a population-based study of 2,449 individuals, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Mok F., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in Southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Mok F., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in Southern Chinese, 4th International Congres of Chinese Orthopaedic Association. Xiamen, China, 2009.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., ISSLS Prize for Lumbar Spine Research. Prevalence, determinants and association of Schmorl’s Nodes of the lumbar spine with disc degeneration: A population-based study of 2499 individuals., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010. 2010.
Mok P.S., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., Prevalence, determinants and association of Schmorl’s nodes of the lumbar spine with disc degeneration: a population-based study of 2449 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Mok P.S., Cheung K.M.C., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl’s nodes of the lumbar spine: prevalence, classification, associated risk factors, and clinical relevance, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Peng S.L., Liu X.W., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., Effect of different timing of strontium treatment on bone remodeling in ovariectomized rats, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Best Clinical Paper Award for Associate Member. Is there a relationship between intervertebral disc degeneration based on MRI and low back pain?, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 4th International Congress of the Chinese Orthopaedic Association. Xiamen, China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the Orthopaedic Research Society. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Determinants of lumbar disc degeneration in older individuals, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Determinants of lumbar disc degeneration in older individuals, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on MRI and low back pain? Best Clinical Paper Award, Hong Kong Orthopaedic Association. 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on MRI and low back pain?, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on magnetic resonance imaging and low back pain, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS). Most Promising Spine Paper Award, Hong Kong Orthopaedic Association. 2009.
Samartzis D., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., Prevalence, determinants, and association of Schmorl's nodes of the lumbar spine with disc degeneration: a population-based study of 2,449 individuals. Best Clinical Study Prize, International Society for the Study of the Lumbar Spine. 2010.
Samartzis D., Karppinen J., Mok P.S., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Skipped level disc degeneration of the lumbar spine, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Samartzis D., Karppinen J., Mok P.S., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Skipped level disc degeneration of the lumbar spine, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 4th International Congress of the Chinese Orthopaedic Association. Xiamen, China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between concomitant cervical and lumbar disc degeneration and associated abnormalities, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between concomitant cervical and lumbar disc degeneration and associated abnormalities, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between overweight / obesity and low back pain in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between overweight/obesity and low back pain in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the Orthopaedic Research Society. New Orleans, Louisiana, USA, 2010.
Saw L.B., Cheung K.M.C., Grozman S.A.M. and Luk K.D.K., A practical classification of the distal radius and ulna radiograph for assessing skeletal maturity, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Tang W.K., Luk K.D.K., Yeung K.W.K. and Lu W.W., In vivo kinematics of the lumbar facet joints for the development of a facet fixator, 2009.
Tang W.K., Yeung K.W.K., Lu W.W. and Luk K.D.K., In-vitro kinematics study of human lumbar facet joints, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Tong W.Y., Yip K., Mo S., Cheung K.M.C., Luk K.D.K. and Yeung K.W.K., Promotion of bone formation at implant interface by local injection of mouse bone marrow-derived mesenchymal stem cells, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Wen C., Cui J., Hu Y. and Luk K.D.K., Loss of Entropy in Fractional Anisotropy Mapping of Spinal Cord in Patients of Cervical Spondylotic Myelopathy., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010. . 2010.
Wen C., Hu Y., Li T.H., Lam K.O., Wu E.X. and Luk K.D.K., Multiple bio-imaging evaluations of spinal cord chronic compression in rat, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A Biodegradable Polymer-Based Coating To Control The Performance Of Magnesium Alloy Orthopaedic Implants, Biomaterials. 2010, 31(8): 2084-209.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., A New PCL Based Porous Membrane to Control the Degradation of Biodegradable Metallic Materials., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug–3Sep 2009. Poster presentation.. 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants biomaterials, Biomaterials. 2010, 31: 2084-2096.
Wong H.M., Yeung K.W.K., Leung Y.L., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., In-vitro analysis of gene expressions of osteoblastic cells while exposing to various concentrations of magnesium ions, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., Reduction of the Corrosion Rate of Biodegradable Metallic Materials by Al2O3 PIII surface Treatment., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24pril2010.. 2010, 868.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.
Wong Y.W., Cheung K.M.C., Cheung W.Y. and Luk K.D.K., The importance of spinal cord monitoring before and after turning the patients prone for operations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., 250. Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009, 17: 1959-1966.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of ninety-one cases, Spine. 2010, 35(6): 635-641.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, Spine. 2010, 35: 635-641.
Yeung C.Y., Leung K.Y., Yeung K.W.K., Kao R.Y.T., Chu P.K., Cheung K.M.C. and Luk K.D.K., Inhibition of metallic implant-related bacterial infections by novel water plasma surface treatment, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Tu H., Chu P.K. and Liu X.Y., Alterations of Surface Mechanics of Nickel Titanium Shape Memory Alloys after Plasma Surface Treatment. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010).. 2010, 110-111.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. PCT Patent Application (No.: PCT/CN2010/000216) Filed: Feb 18, 2010., PCT Patent Application. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010., U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010.. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, PCT Patent Application (No.: PCT/CN2010/000216) filed 18 February 2010. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, U.S. Non-Provisional Patent (No.: 12/706,483). filed 16 February 2010. 2010.
Yeung K.W.K., Lai D.W.T., Lai D.W.T., Lai D.W.T., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Wu S.L., Tu H., Chu P.K. and Chung C.Y., Biomechanical Analysis of Plasma Treated NiTi Materials for Spinal Deformity Correction., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May 2010). 2010, 24-25.
Yeung K.W.K., Lui S.C., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of Cell Adhesion and Proliferation on Polyetheretherketone by Using Plasma Surface Modification., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24April2010.. 2010, 864.
Yeung K.W.K., Leung K.Y., Kao R.Y.T., Chu P.K., Yip K., Luk K.D.K. and Cheung K.M.C., Feasibility Study of a New Plasma Coating to Suppress Bacterial Adhesion in Orthopaedic, Proceedings 4th International Conference on Technological Advances of Thin Films and Surface Coatings (ThinFilms 2008) (July 13-16, 2008). 2009, (Paper 4150) 35.
Yeung K.W.K., Lam K.O., Luk K.D.K., Cheung K.M.C., Wu S.L., Hu T., Liu X.Y., Chu C.L., Chu P.K. and Chan D., Long Term Cytocompatibility and In vivo Investigation of Nitrogen Plasma Implanted Shape Memory Alloy. , International Conference on Shape Memory ands Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 104-105.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K. and Chung C.Y., Metallic Implants for Spinal Deformity Correction, Advances in Biomedical Sciences and Engineering. USA, Bentham Science Publisher Ltd, 2009, 1-44.
Yeung K.W.K., Lam W.N., Natarajan D., Wu S.L., Hu T., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Nickel Ion Level in Scoliotic Patients Implanted with Nitrogen Plasma Surface Modified Nickel-Titanium Superelastic Spinal Implant., 3rd IEEE International NanoElectronics Conference (INEC), Hong Kong, 3-8 January 2010. Oral presentation.. 2010, Paper BC410, p. 134.
Yeung K.W.K., Kao R.Y.T., Chu P., Luk K.D.K. and Cheung K.M.C., Oxygen Plasma Implanted Titanium Surface to Resist Staphylococcus Aureus Adhesion., 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep 2009. Oral presentation.. 2009.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Wu S.L., Liu X.Y., Chu C.L., Chung C.Y. and Chu P., Plasma Surface Modification of Nickel Titanium Shape Memory Alloys, Advances in Biomedical Sciences and Engineering. USA, Bentham Science Publisher Limited, 2009, 45-68.
Yeung K.W.K., Lam W.N., Wu S.L., Tu H., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Post-Operative Monitoring of Nickel Ion Level in Scoliotic Patients Operated with Novel Nickel-Titanium Superelastic Spinal Implant. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20 May 2010). 2010, 22-23.
Yeung K.W.K., Wu S.L., Tu H., Chu P.K., Liu X.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Suppression of Surface Nickel Concentration of Nickel Titanium Shape Memory Alloys by Plasma Surface Treatment., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 112-113.
Zhang Z., Luk K.D.K. and Hu Y., Identification of detailed time-frequency components in somatosensory evoked potentials, IEEE Transactions on Neural Systems and Rehabilitation Engineering. 2010, 18: 245-254.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: Methods and comparisons. , J Electromyogr Kinesiol.. 2010, 20: 89-101.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: methods and comparisons, Journal of Electromyography and Kinesiology. 2010, 20: 89-101.
Zhao X., Li Z., Liu W.G., Pan H., Lam R.W.M., Luk K.D.K. and Lu W.W., Characterization and evaluation of octa-arginine conjugated chitosan as non-biral vector for gene transfer, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Zheng Z.M., Yu B.S., Chen H., Aladin Kaderbatcha D.M., Zhang K.B., Zhang J.F., Liu H., Luk K.D.K. and Lu W.W., 251. Effect of iliac screw insertion depth on the stability and strength of lumbo-iliac fixation constructs. An anatomical and biomechanical study, Spine. 2009, 34(16): E565-E572.


Researcher : Ma W

List of Research Outputs

Ma W., Ng F.Y., Chiu P.K.Y., Li Z. and Lu W.W., The effects of acetabular component position and hip ball diameter on posterior stability after total hip replacement, Chinese Journal Of Joint Surgery. 2009, 3: 624-629.


Researcher : Mak JNF

List of Research Outputs

Hu Y., Siu H.F., Mak J.N.F. and Luk K.D.K., 256. Lumbar muscle electromyographic dynamic topography during flexion-extension, Journal of Electromyography and Kinesiology. 2010, 20: 246-255.


Researcher : Mak KH

List of Research Outputs

Fong D.Y.T., Lee C.F., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., A meta-analysis of the clinical effectiveness of school scoliosis screening, Spine (Phila Pa 1976). 2010, 35(10): 1061-1071.
Lee C.F., Fong D.Y.T., Cheung K.M.C., Cheng J.C.Y., Ng B.K.W., Lam T.P., Mak K.H., Yip P.S.F. and Luk K.D.K., Costs of School Scoliosis Screening: A Large, Population-Based Study, Spine (Phila Pa 1976). 2010, Epub ahead of print.


Researcher : Mak NFJ

List of Research Outputs

Hu Y., Siu H.F., Mak N.F.J. and Luk K.D.K., Lumbar Muscle Electromyographic Dynamic Topography During Flexion-extension., J Electromyogr Kinesiol.. 2010, 20: 246-55.
Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment. , The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK).. Aalborg Denmark., 2010.
Hu Y., Mak N.F.J., Kwok H.Y. and Luk K.D.K., Surface electrography(semg) topography for low back pain assessment., The XVIII International Conference of the Society of Electrophysiology and Kinesiology (ISEK). 16-19 June, 2010, Aalborg Denmark. 2010.


Researcher : Mok PS

List of Research Outputs

Cheung K.M.C., Mok P.S., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl's nodes of the lumbar spine: prevalence, classification, associated risk factors and clinical relevance, 83rd Annual Meeting of the Japanese Orthopaedic Association, Tokyo, Japan, May 27-30, 2010.
Cheung K.M.C., Samartzis D., Karppinen J., Mok P.S., Ho D.W.H., Fong D.Y.T. and Luk K.D.K., “Skipped” level disc degeneration of the lumbar spine, a MRI study of 1989 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., ISSLS Prize for Lumbar Spine Research. Prevalence, determinants and association of Schmorl’s Nodes of the lumbar spine with disc degeneration: A population-based study of 2499 individuals., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010. 2010.
Mok P.S., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., Prevalence, determinants and association of Schmorl’s nodes of the lumbar spine with disc degeneration: a population-based study of 2449 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Mok P.S., Cheung K.M.C., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl’s nodes of the lumbar spine: prevalence, classification, associated risk factors, and clinical relevance, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Samartzis D., Karppinen J., Mok P.S., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Skipped level disc degeneration of the lumbar spine, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Samartzis D., Karppinen J., Mok P.S., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Skipped level disc degeneration of the lumbar spine, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Natarajan D

List of Research Outputs

Cheung K.M.C., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Cheung K.M.C., Samartzis D., Natarajan D., Li J., Cheung W.Y., Lenke L.G. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in scoliosis correction with alternate level pedicle screw fixation, Journal of Bone and Joint Surgery. 2010, 92: 169-176.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation., 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.. 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, J Bone Joint Surg (Am). 2010, 92: 169-76.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Spine Chapter's Most Promising Spine Paper Award. Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Leung Y., Natarajan D., Samartzis D., Borkhuu B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis (AIS) using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Lam W.N., Natarajan D., Wu S.L., Hu T., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Nickel Ion Level in Scoliotic Patients Implanted with Nitrogen Plasma Surface Modified Nickel-Titanium Superelastic Spinal Implant., 3rd IEEE International NanoElectronics Conference (INEC), Hong Kong, 3-8 January 2010. Oral presentation.. 2010, Paper BC410, p. 134.


Researcher : Ng FY

List of Research Outputs

Chiu P.K.Y., Ng F.Y., Lee O.B., Tsang W.L., Wong H.L., Leung K.H., Wu W.C., Tang W.M., Chan W.S. and Lau W.L., A prospective, randomized, inter-regional, multi-centre study on gender solutions knee prostheses in Chinese females, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.
Kuong E., Ng F.Y., Yan C.H., Chiu P.K.Y. and Fang C., Antibiotic Prophylaxis After Total Joing Replacements, Hong Kong Medical Journal. 2009, 15: 458-62.
Ma W., Ng F.Y., Chiu P.K.Y., Li Z. and Lu W.W., The effects of acetabular component position and hip ball diameter on posterior stability after total hip replacement, Chinese Journal Of Joint Surgery. 2009, 3: 624-629.
Ng F.Y., Chiu P.K.Y. and Yan C.H., Anterior Dislocation After Total Hip Replacement - Effects Of Pelvic Rotation And Hip Ball Size, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Ng F.Y., Jiang J.T., Chiu P.K.Y., Yan C.H. and Choi C.L., Anterior dislocation after total hip replacement - effects of pelvic rotation and hip ball size , The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S56.
Ng F.Y., Chiu P.K.Y., Yan C.H. and Ng K.F., Pain control after total knee replacement - comparison of local anaesthetics via continuous femoral catheter with patient-controlled analgesia, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.
Ng F.Y., Chiu P.K.Y. and Yan C.H., Posterior Dislocation After Total Hip Replacement - Effects Of Position Of Acetabular Shell And Hip Ball Size, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., Posterior dislocation after total hip replacement - the effect of position of acetabular shell and hip ball size, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S33.
Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., 全髋關節置換術後的前脫位- 骨盆旋轉和股骨頭大小的影響, The Eleventh National Congress Of Orthopaedic Surgery And The Fourth International Congress Of Chinese Orthopaedic Association. 2009, 64-65.
Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented Revision Total Hip Arthroplasty Using Impaction Bone Grafting And Elite Plus Stem: A Retrospective Review Of 15 Cases, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented revision total hip arthroplasty using impaction bone grafting and Elite plus stem: A retrospective review of 15 cases, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S58.
Yan C.H., Chiu P.K.Y. and Ng F.Y., QMH Experience in Primary TKR for Primary Knee Osteoarthritis 2000 - 2009, 7th Hong Kong International Orthopaedic Forum. 2010.
Yan C.H., Chiu P.K.Y. and Ng F.Y., 36毫米股骨頭在國人全髋置換中之效果, The Eleventh National Congress Orthopaedic Surgery And The Fourth International Congress Of Chinese Orthopaedic Association. 2009, 57.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The Radiographic Prevalence Of Knee Osteoarthritis In Local Chinese Subjects: A Retrospective Review Of 102 Angiogram Images, 29th Annual Congress Of The Hong Kong Orthopaedics Association. 2009.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The radiographic prevalence of knee osteoarthritis in local Chinese subjects: a retrospective review of 102 angiogram images, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S56.
Yan C.H., Chiu P.K.Y. and Ng F.Y., Total Hip Arthroplasty with 36mm Articulation in Chinese Patients, Annual Congress of Hong Kong Orthopaedic Association. 2009.


Researcher : Ng TP

List of Research Outputs

Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented Revision Total Hip Arthroplasty Using Impaction Bone Grafting And Elite Plus Stem: A Retrospective Review Of 15 Cases, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented revision total hip arthroplasty using impaction bone grafting and Elite plus stem: A retrospective review of 15 cases, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S58.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The Radiographic Prevalence Of Knee Osteoarthritis In Local Chinese Subjects: A Retrospective Review Of 102 Angiogram Images, 29th Annual Congress Of The Hong Kong Orthopaedics Association. 2009.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The radiographic prevalence of knee osteoarthritis in local Chinese subjects: a retrospective review of 102 angiogram images, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S56.


Researcher : Ni G

List of Research Outputs

Ni G., Lin J.H., Chiu P.K.Y., Li Z. and Lu W.W., Effect of strontium-containing hydroxyapatite bone cement on bone remodeling following hip replacement, Journal Of Material Science,. Materials In Medicine. 2010, 21: 377-384.


Researcher : Pan H

Project Title:Nano Strontium involved Natural Coral as Novel Biomaterials
Investigator(s):Pan H, Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Small Project Funding
Start Date:12/2009
Abstract:
Aims of this project: 1) Select coral as natural source for bone tissue engineering as novel biomaterial, supported by Shanghai Ocean University for coral sources) 2) To synthesize nano strontium-incorporated coral by hydrothermal treatment, remaining interconnected structure and high mechanical strength 3) To evaluate biocompatibility and mechanics of this novel biomimetic product in vitro follow ISO standard. Key points and challenges: 1) The treatment of osteoporosis is still a globally and locally challenge, although some drugs such as strontium ranelate (SrR) can increase bone mineral density, no effectiveness can be achieved in decreasing bone porosity. The related surgical operations are technically complex and high cost. In particular, the conventional polymethylmethacrylate (PMMA)-contained bone cement, currently widely used as bone filler, still triggers lots of side effects (e.g. thermal and chemical bone damages) due to its non-bioactive nature, monomer cytotoxicity and high setting temperature, as indicated by the FDA in a November 2002 warning notice. Therefore, the development novel bioactive material is in great urgent and necessary, which should be bioactive, nontoxic, high mechanical strength comparable to cancellous bone, meanwhile the porous structure induce bone ingrowth. 2) Bioactive and induce bone formation Upon above requirement, coral is such a material, showing bioactive because it is a natural mineral, non-toxicity, the porous structure with pore size between 100-500 µm is necessary for bone ingrowth. Therefore, coral has been paid more attention in bone tissue engineering as natural bone filler for twenty years. The novelty in this work lies in the incorporation of strontium, the release of strontium can stimulate new bone formation. 3) Low costs for massive production Coral is a kind of animal that lives in the ocean. It is hard and comes in many colors and structures with different sizes and shapes. Although arbitrary logging, the protection of coral is necessary, the sufficient source still can provide enough for scientific study and further industrial application. 4) Structure close to human bone and highly biocompatibility The increased interest using coral as bone substitution material lies in its high mechanical property similar to cancellous bone with high porosity. The synthetic scaffold always suffers the problem of lower mechanical strength, although it is highly porous. The advantage of coral is therefore structurally close to cancellous bone, particularly with high mechanical strength. Due to as natural animal, it is highly biocompatibility. 5) Technical challenge The selection of coral is the first and most important step since hundreds of coral can be collected in sea, but grown under different condition, e.g. temperature, sea composition, pH. Osteoblast cell may proliferate and grow on it, but depends on its unique structure and composition. Therefore, under the help of Shanghai Ocean University, the selection and preliminary evaluation of its bioactivity and biocompatibility are necessary. In addition, the synthetic condition of strontium incorporation may vary due to different situation such as synthetic temperature, pH and time, which return to affect the incorporation of strontium.


List of Research Outputs

Lam R.W.M., Pan H., Li Z., Chen Y., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Fatty acid affects the nucleation of strontium-substituted biominerals, Ceram Int. 2010, 36(2): 683-688.
Lam W.M.R., Pan H., Li Z., Yang C., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Strontium-substituted calcium phosphates prepared by hydrothermal method under linoleic acid-ethanol solution, CERAMICS INTERNATIONAL. 2010, 36(2): 683-688.
Li Z., Pan H., Luk K.D.K. and Lu W.W., Bone Mechanical Properties Determination from Macro- to Nanoscopic Scale, The 3rd China International Congress and Exibition of Orthopaedics. 2009.
Liu X., Xie Z., Zhang C., Pan H., Rahaman M., Zhang X., Fu Q. and Huang W., Bioactive borate glass scaffolds: in vitro and in vivo evaluation for use as a drug delivery system in the treatment of bone infection, J Mater Sci: Mater Med . 2010, 21: 575-582.
Lui S.C., Yeung K.W.K., Chu P.K., Kwok T.K., Pan H., Luk K.D.K. and Cheung K.M.C., Plasma-modified polyetheretherketone for orthopaedic applications: bulk and surface mechanical characterisations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Pan H., Lam W.M.R., Zhao X., Li Z., Lu W.W., Wang D., Huang W., Lin K. and Chang J., Borosilicate for tissue engineering, World Association for Chinese Biomedical Engineers (WACBE). 2009.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Pan H. and Darvell B.W., Solubility of tetra-calcium phosphate (TTCP) and tricalcium phosphate (β-TCP) by solid titration, Arch Oral Biol. 2009, 54(7): 671-677.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.
Zhao X., Li Z., Liu W.G., Pan H., Lam R.W.M., Luk K.D.K. and Lu W.W., Characterization and evaluation of octa-arginine conjugated chitosan as non-biral vector for gene transfer, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Peng SL

List of Research Outputs

Luk K.D.K., Lu W.W., Zhou G., Peng S.L., Wang T. and Li Z., New Investigator Recognition Award., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010. 2010.
Peng S.L., Liu X.W., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., Effect of different timing of strontium treatment on bone remodeling in ovariectomized rats, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Poon TL

List of Research Outputs

Cheung K.M.C., Yeung K.W.K., Lu W.W., Poon T.L. and Chow S.P., Fixation device, US Regular patent no. 7,695,471, granted on April 13, 2010, 2010.


Researcher : Samartzis D

List of Research Outputs

Chang K.C., Samartzis D., Luk K.D.K. and Cheung K.M.C., Cervical spine disease in Asian populations, Hong Kong Medical Journal. 2010, 16: 69-70.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S.S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Cheung K.M.C., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Cheung K.M.C., Samartzis D., Natarajan D., Li J., Cheung W.Y., Lenke L.G. and Luk K.D.K., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in scoliosis correction with alternate level pedicle screw fixation, Journal of Bone and Joint Surgery. 2010, 92: 169-176.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation., 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.. 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, J Bone Joint Surg (Am). 2010, 92: 169-76.
Cheung K.M.C., Mok P.S., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl's nodes of the lumbar spine: prevalence, classification, associated risk factors and clinical relevance, 83rd Annual Meeting of the Japanese Orthopaedic Association, Tokyo, Japan, May 27-30, 2010.
Cheung K.M.C., Samartzis D., Karppinen J., Mok P.S., Ho D.W.H., Fong D.Y.T. and Luk K.D.K., “Skipped” level disc degeneration of the lumbar spine, a MRI study of 1989 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Danon-Hersch N., Samartzis D., Wietlisbach V., Porchet F. and Vader J., Appropriateness criteria for improved surgery outcomes in patients with low back pain and/or sciatica, Spine. 2010, 35: [Epub ahead of print].
Fok A., Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The prevalence, factors, and clinical profile associated with Tarlov cysts in Southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Fok A.W.M., Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The prevalence, factors, and clinical profile associated with tarlov cysts in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kim M., Chan Q., Anthony M., Cheung K.M.C., Samartzis D., Chan T. and Khong P.L., Assessment of glycosaminoglycan distribution in human lumbar intervertebral discs using chemical exchange saturation transfer, Joint ISMRM-ESMRMB Annual Scientific Meeting, Stockholm, Sweden, 1-7 May . 2010.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Lenke L., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS), 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Spine Chapter's Most Promising Spine Paper Award. Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Leung Y., Natarajan D., Samartzis D., Borkhuu B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis (AIS) using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Li J., Cheung K.M.C., Samartzis D., Zhu X., Li M. and Luk K.D.K., Key –Vertebral Screw Strategy (KVSS): an alternative surgical strategy for main thoracic curve correction in patients with adolescent idiopathic scoliosis, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Mok F., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., 2010 ISSLS Prize Winner: Prevalence, determinants, and association of Schmorl's nodes of the lumbar spine with disc degeneration: a population-based study of 2,449 individuals, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Mok F., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in Southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Mok F., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in Southern Chinese, 4th International Congres of Chinese Orthopaedic Association. Xiamen, China, 2009.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., ISSLS Prize for Lumbar Spine Research. Prevalence, determinants and association of Schmorl’s Nodes of the lumbar spine with disc degeneration: A population-based study of 2499 individuals., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010. 2010.
Mok P.S., Samartzis D., Karppinen J., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Modic changes of the lumbar spine in southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Mok P.S., Samartzis D., Karppinen J., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., Prevalence, determinants and association of Schmorl’s nodes of the lumbar spine with disc degeneration: a population-based study of 2449 individuals, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Mok P.S., Cheung K.M.C., Samartzis D., Karppinen J., Cauilan G.E., Luk K.D.K. and Fong D.Y.T., Schmorl’s nodes of the lumbar spine: prevalence, classification, associated risk factors, and clinical relevance, 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.
Samartzis D., Wein S., Shen F.H., Beazell J., Francke E. and Anderson D.G., A revisitation of distractive-extension injuries of the subaxial cervical spine: a cadaveric and radiographic soft tissue analysis, Spine. 2010, 35: 395-402.
Samartzis D., AO Spine Global Spine Congress Best Poster Award. Juvenile disc degeneration: epidemiology, risk factors, and clinical relevance, Global Spine Congress, San Francisco, California, USA. 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Shen F.H., Herman J. and Mardjetko S.M., Atlantoaxial rotatory fixation in the setting of associated congenital malformations: a modified classification system, Spine. 2010, 35: E119-E127.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Best Clinical Paper Award for Associate Member. Is there a relationship between intervertebral disc degeneration based on MRI and low back pain?, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 4th International Congress of the Chinese Orthopaedic Association. Xiamen, China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, Annual Meeting of the Orthopaedic Research Society. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Body mass index and its association with disc degeneration of the lumbar spine in adults, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Samartzis D., Cervical Arthroplasty vs. ACDF: a Meta-Analysis, Peking Union Medical College, Beijing, China. 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Determinants of lumbar disc degeneration in older individuals, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Determinants of lumbar disc degeneration in older individuals, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Is Motion Preservation Better than Fusion for Cervical Disc Disease, Hong Kong Orthopaedic Forum, Hong Kong. 2010.
Samartzis D., Is motion preservation better than fusion for cervical disc disease?, 7th Hong Kong International Orthopaedic Forum. Hong Kong, 2010.
Samartzis D., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on MRI and low back pain? Best Clinical Paper Award, Hong Kong Orthopaedic Association. 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on MRI and low back pain?, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., Is there a relationship between intervertebral disc degeneration based on magnetic resonance imaging and low back pain, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Meta-Analysis in Spine Research, Peking Union Medical College. 2010.
Samartzis D., New Insights on Disc Degeneration and Low Back Pain, Department of Orthopaedic Surgery, Navy General Hospital, Beijing, China. 2010.
Samartzis D., New Insights on Disc Degeneration and Low Back Pain, Peking Union Medical College. 2010.
Samartzis D., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis (AIS). Most Promising Spine Paper Award, Hong Kong Orthopaedic Association. 2009.
Samartzis D., Luk K.D.K., Fong D.Y.T. and Cheung K.M.C., Prevalence, determinants, and association of Schmorl's nodes of the lumbar spine with disc degeneration: a population-based study of 2,449 individuals. Best Clinical Study Prize, International Society for the Study of the Lumbar Spine. 2010.
Samartzis D., Karppinen J., Mok P.S., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., Skipped level disc degeneration of the lumbar spine, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Samartzis D., Nishi N., Hayashi M., Cologne J., Kodama K., Miles E.F., Funamoto S., Suyama A., Soda M. and Kasagi F., Soft tissue sarcomas in atomic-bomb survivors of Hiroshima and Nagasaki, 16th Hong Kong International Cancer Congress and 6th Annual Meeting Centre for Cancer Research‏. Hong Kong, SAR China, 2009.
Samartzis D., Nishi N., Hayashi M., Cologne J., Kodama K., Miles E.F., Funamoto S., Suyama A., Soda M. and Kasagi F., Soft tissue sarcomas in atomic-bomb survivors of Hiroshima and Nagasaki, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Nishi N., Hayashi M., Cologne J., Kodama K., Miles E.F., Funamoto S., Suyama A., Soda M. and Kasagi F., Soft tissue sarcomas in atomic-bomb survivors of Hiroshima and Nagasaki, 3rd Joint Scientific Meeting of The Royal College of Radiologists & Hong Kong College of Radiologists and the17th Annual Scientific Meeting of Hong Kong College of Radiologists. Hong Kong, SAR China, 2009.
Samartzis D. and LaMarca F., Spinal flexibility in scoliosis: roots "bending" to antiquity, Spine J. 2010, 10: 277-278.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, 4th International Congress of the Chinese Orthopaedic Association. Xiamen, China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between cigarette smoking and intervertebral disc degeneration of the lumbar spine, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between concomitant cervical and lumbar disc degeneration and associated abnormalities, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between concomitant cervical and lumbar disc degeneration and associated abnormalities, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between overweight / obesity and low back pain in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Samartzis D., Karppinen J., Luk K.D.K. and Cheung K.M.C., The association between overweight/obesity and low back pain in adults, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Samartzis D., The association of ionizing radiation and bone sarcomas: new insights based on atomic-bomb survivors of Hiroshima and Nagasaki. Best Poster Award, Chinese Orthopaedic Association. 2009.
Samartzis D., Nishi N., Hayashi M., Cologne J., Sakamoto Y., Cullings H.M., Kodama K., Miles E.F., Funamoto S., Suyama A., Soda M. and Kasagi F., The association of ionizing radiation and bone sarcomas: new insights based on atomic-bomb survivors of Hiroshima and Nagasaki, 4th International Congress of the Chinese Orthopaedic Association. Xiamen, China, 2009.
Samartzis D., Travel Award, Mainland China Visiting Lecturer/Professorship. 2010.
Samartzis D., Traveling Fellow Scholarship Award, Hong Kong Orthopaedic Association. 2009.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the American Academy of Orthopaedic Surgeons. New Orleans, Louisiana, USA, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the International Society for the Study of the Lumbar Spine. Auckland, New Zealand, 2010.
Samartzis D., Karppinen J., Mok F., Fong D.Y.T., Luk K.D.K. and Cheung K.M.C., “Skipped” level disc degeneration of the lumbar spine: new insights on the etiology & clinical relevance association with disc degeneration, Annual Meeting of the Orthopaedic Research Society. New Orleans, Louisiana, USA, 2010.
Vavken P. and Samartzis D., Efficacy of autologous chondrocyte implantation in cartilage repair of the knee: a systematic review of randomized controlled trials, Osteoarthritis Cartilage. 2010, 18: 857-863.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of ninety-one cases, Spine. 2010, 35(6): 635-641.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, Spine. 2010, 35: 635-641.


Researcher : Saw LB

List of Research Outputs

Saw L.B., Cheung K.M.C., Grozman S.A.M. and Luk K.D.K., A practical classification of the distal radius and ulna radiograph for assessing skeletal maturity, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.


Researcher : Siu HF

List of Research Outputs

Hu Y., Siu H.F., Mak N.F.J. and Luk K.D.K., Lumbar Muscle Electromyographic Dynamic Topography During Flexion-extension., J Electromyogr Kinesiol.. 2010, 20: 246-55.
Hu Y., Siu H.F., Mak J.N.F. and Luk K.D.K., 256. Lumbar muscle electromyographic dynamic topography during flexion-extension, Journal of Electromyography and Kinesiology. 2010, 20: 246-255.
Hu Y., Siu H.F. and Luk K.D.K., Quantitative surface electromyography topography for low back pain assessment, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.


Researcher : Siu HF

List of Research Outputs

Hu Y., Siu H.F., Mak N.F.J. and Luk K.D.K., Lumbar Muscle Electromyographic Dynamic Topography During Flexion-extension., J Electromyogr Kinesiol.. 2010, 20: 246-55.
Hu Y., Siu H.F., Mak J.N.F. and Luk K.D.K., 256. Lumbar muscle electromyographic dynamic topography during flexion-extension, Journal of Electromyography and Kinesiology. 2010, 20: 246-255.
Hu Y., Siu H.F. and Luk K.D.K., Quantitative surface electromyography topography for low back pain assessment, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.


Researcher : Tam V

List of Research Outputs

Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Chan K.Y., Lu W.W., Sze K.Y., Leung Y.L., Tam V. and Cheung K.M.C., Development of decellularized intervertebral disc scaffold for tissue engineering, Orthopaedic Research Society. 2010.
Chan K.Y., Leung Y.L., Tam V., Lu W.W., Sze K.Y. and Cheung K.M.C., Production of a natural disc scaffold for intervertebral disc tissue engineering, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Tong W.Y.T., Liang Y.M., Tam V., Yip H.K., Kao R.Y.T., Cheung K.M.C., Yeung K.W.K. and Lam Y.W., Biochemical characterization of the cell-biomaterial interface by quantitative proteomics, Mol Cell Proteomics. 2010.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A Biodegradable Polymer-Based Coating To Control The Performance Of Magnesium Alloy Orthopaedic Implants, Biomaterials. 2010, 31(8): 2084-209.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants biomaterials, Biomaterials. 2010, 31: 2084-2096.


Researcher : Tang WK

List of Research Outputs

Lu W.W., Tang W.K., Yeung K.W.K. and Luk K.D.K., In vitro kinematics study of human lumbar facet joints, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Tang W.K., Luk K.D.K., Yeung K.W.K. and Lu W.W., In vivo kinematics of the lumbar facet joints for the development of a facet fixator, 2009.
Tang W.K., Yeung K.W.K., Lu W.W. and Luk K.D.K., In-vitro kinematics study of human lumbar facet joints, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Tang WK

List of Research Outputs

Lu W.W., Tang W.K., Yeung K.W.K. and Luk K.D.K., In vitro kinematics study of human lumbar facet joints, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Tang W.K., Luk K.D.K., Yeung K.W.K. and Lu W.W., In vivo kinematics of the lumbar facet joints for the development of a facet fixator, 2009.
Tang W.K., Yeung K.W.K., Lu W.W. and Luk K.D.K., In-vitro kinematics study of human lumbar facet joints, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Tang WM

List of Research Outputs

Chiu P.K.Y., Ng F.Y., Lee O.B., Tsang W.L., Wong H.L., Leung K.H., Wu W.C., Tang W.M., Chan W.S. and Lau W.L., A prospective, randomized, inter-regional, multi-centre study on gender solutions knee prostheses in Chinese females, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.
Chiu P.K.Y., Cheng H.C., Yau W.P., Tang W.M. and Ho H.S., Reading Radiographs after Total Knee Arthroplasty, Hong Kong Journal of Orthopaedic Surgery . 2010, 14 (1): 25 - 39.
Ng J.K.F., Lawmin J.C., Tsang S.F., Tang W.M. and Chiu P.K.Y., The value of a single preoperative PFA-100® measurement in assessing the risk of bleeding in patients taking cyclooxygenase inhibitors and undergoing total knee replacement, British Journal of Anaesthesia. 2009, 102: 779-84.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.
Zhao Z.Y., Chiu P.K.Y., Zhang S.D., Ma W.H., Yau W.P., Tang W.M., Wang S.J. and Wang W.G., A study on the effects of tibial cutting jig fixation on varus-valgus and anteroposterior slope of the tibial osteotomy, 胫骨平台切朴的因定方法对胫骨平台截骨在内外翻和前后倾方面影响的研究, Chinese Journal of Joint Surgery . 中国关节外科杂志, 2009, 3: 750 - 754.


Researcher : To MKT

Project Title:Deformity correction at the diaphysis of the long bone using a shape memory alloy plate
Investigator(s):To MKT, Yeung KWK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:12/2009
Abstract:
Purpose: This study is to design a pilot study to investigate a new method of deformity correction of long bone using a shape memory alloy plate as a gradual bone modeling device. The study will include a prototype development of the device, mechanical characterization of the material, and a pilot animal study. Hypothesis: The superelasticity and shape memory properties of the shape memory alloy can be used to model an intact long bone. Introduction: Limb deformity in paediatric patients1-5 can be due to congenitial anomalies, physiological development, malunion after fracture, bone dysplasia, physeal abnormality and metabolic diseases. Depending on the site of the deformity, such deformity can pose functional, social and cosmetic problems to the patients. Though the remodeling potential of the immature skeleton in paediatric patients allows some of the limb deformity to remodel, those fail to remodel before adulthood will persist. The remodeling potential of a long bone is governed by the site of the deformity and the plane of the deformity in relation to the neighbouring joint. According to Wolff’s law6, bone remodels in response to mechanical stress. Increasing mechanical stress leads to significant bone gain and removing external mechanical stress can lead to significant bone loss7-10. We would like to utilize this biomechanical property of the bone in deformity correction. Deformity correction in an immature long bone largely depends on the site and severity of the deformity as well as the remaining growth potential of the children. Surgical correction of the deformity can be either an acute or gradual correction. The acute correction11 includes cutting open the bone at the deformity, immediate correction of the deformity, followed by fixation of the bone with an implant or external fixator. These surgeries are usually complicated and carry certain morbidities12-14 including the surrounding neurovascular injury. Patients are not allowed to weight-bear after the surgery because the fixations are not stable. Gradual correction, on the other hand, includes cutting open the bone at the deformity and fixing the bone using an external fixator. The deformity is corrected by adjusting the external fixator after the surgery until it is fully corrected. The correction is usually spread out in terms of weeks or even months. The bone takes more than 6 weeks to heal after stopping the adjustment and at least 3 months to consolidate. Similar to the acute correction, patients are not allowed to weight-bear because the fixations are not stable. The complications related to the use of external fixation are significantly higher than internal fixation (implants). These include pin tract infection, unsightly scaring, and patients’ discomfort. An alternate method is to control the remaining growth of the long bone in order to correct the deformity. Hemiepiphysiodesis15,16, stapling17 and eight plates18 are commonly used as a guided growth control method or device. By suppressing the growth of part of the physis, the remaining physis will produce an asymmetric growth to compensate the original deformity. Such method is good for patients with significant growth potential remaining and with deformity near the growth plate. The drawback of this method is that the correction is slow and the effect will not be obvious until at least one year after the surgery. However, this method is less invasive with less morbidity and patients can resume full weight bearing immediate after surgery. Our department is the pioneer in using shape memory alloy in the scoliosis surgery. We have obtained much experience and knowledge over the past few years in its biomechanical property. We believe that shape memory alloy has a very good potential in long bone deformity correction and we would like to investigate on this. Shape memory alloy is a special material that has a superelastic and shape memory properties where it can deform more than normal materials, yet able to recover its original shape upon unloading. There are three main types of shape memory alloys, copper-zinc-aluminum-nickel, cooper-aluminum-nickel, and nickel titanium. Nitinol (Nickel Titanium Naval Ordnance Laboratory) is the most commonly used shape memory alloy due to its biocompatibility. Its application is wide including compression bone staples and clamps19-23, suture anchors used in tendon and ligament repair, rods for correction of scoliosis24,25, and Ti-Ni clamps in small bone surgery26,27. The shape memory effect is a unique property of these alloys where it undergoes a complex crystalline solid phase change called martensite-austenite transformation. When the alloy is heated up to a high temperature to reach the austenite phase, the metal stiffens and the shape is “memorized”. When the metal is cooled to a lower temperature, it reaches the martensite phase. The metal becomes less stiff and more malleable. But the metal will recover its original shape and stiffness when it is heated up to the high-temperature austenite phase. Not only does the shape of the metal changes during the phase transformation, significant force is generated when the metal changes from martensite to austenite phase known as shape memory effect. The bending force of the shape memory alloy generated by the nitinol intramedullary rods had been shown to be able to produce bending in an intact tibia of rabbits28. Since the bending force generated by the shape memory alloy is gradual and continuous, the superelasticity and shape memory effect of this alloy has good potential to be used as a gradual bone-modeling device. At present, the knowledge of the use of shape memory alloy as a long bone deformity correction device is limited. Even though long bone modelling has been shown to be possible using a shape memory rod28. The amount of modeling generated is rather less. The insertion of the rod through the physis may potentially damage the growth potential of the physis, giving rise to growth retardation or angular deformity. Therefore, the clinical application of shape memory alloy intrameduallary rod may be limited in long bone deformity correction.


List of Research Outputs

El-fiky T., Chow W., Li Y.H. and To M.K.T., Hereditary multiple exostoses of the hip, In: David Fang, Journal of Orthopaedic Surgery. Hong Kong, Journal of Orthopaedic Surgery, 2009, 17: 161-165.
To M.K.T., AO Course for Operating Room Personnels, In: Winnie Chan, AO Foundation. 2010.
To M.K.T., AO Course for Surgeons: Advances in Paediatric Fractures and Minimal Invasive Osteosynthesis , In: Frankie Leung, AO Foundation. 2010.
To M.K.T., Chow W. and Umrani S., Early radiological results of the interlocking triple innominate osteotomy for hip dysplasia in adolescents, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
To M.K.T., Chow W. and Eid M., Hodgson Award, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
To M.K.T., Hong Kong International Orthopaedic Forum, Department of Orthopaedics & Traumatology, HKU. 2010.
To M.K.T., Long Bone Deformity Correction Using Shape Memory Alloy Plate, CRDG & SRT Development and Reproduction joint seminar. 2010.
To M.K.T., Chow W., Li Y.H. and Eid M., Long-term efficacy of orthotic devices in children with flexible pes planus, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
To M.K.T., Chow W., Choi K.Y., Cheung H.M. and Liu K.L., Supracondylar fracture of humerus in paediatric and adolescent patients of Hong Kong, In: Michael To, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
To M.K.T., Chow W. and Tong S., Tong S, Chow W, To M. Ultrasound assessment and screening of developmental dysplasia of hips in Hong Kong, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Tong WY

List of Research Outputs

Tong W.Y., Yip K., Mo S., Cheung K.M.C., Luk K.D.K. and Yeung K.W.K., Promotion of bone formation at implant interface by local injection of mouse bone marrow-derived mesenchymal stem cells, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.


Researcher : Tong WYT

List of Research Outputs

Tong W.Y.T., Liang Y.M., Tam V., Yip H.K., Kao R.Y.T., Cheung K.M.C., Yeung K.W.K. and Lam Y.W., Biochemical characterization of the cell-biomaterial interface by quantitative proteomics, Mol Cell Proteomics. 2010.


Researcher : Tsang WL

List of Research Outputs

Chiu P.K.Y., Ng F.Y., Lee O.B., Tsang W.L., Wong H.L., Leung K.H., Wu W.C., Tang W.M., Chan W.S. and Lau W.L., A prospective, randomized, inter-regional, multi-centre study on gender solutions knee prostheses in Chinese females, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.


Researcher : Tsui YK

List of Research Outputs

Cheng H.W., Tsui Y.K., Cheung K.M.C., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres - a 3D model to study the effects of acellular matrix on stem cell fate, Tissue Engineering Part C Methods . 2009, 15(4): 697-706.
Cheng H.W., Tsui Y.K., Cheung K.M.C., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres. , WACBE (World Association for Chinese Biomedical Engineers) World Congress on Bioengineering 2009. 26-29 Jul 2009, Hong Kong, China. . 2009, 58.
Cheng H.W., Tsui Y.K., Cheung K.M., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres: a three-dimensional model to study the effects of acellular matrix on stem cell fate , Tissue Engineering, Part C Methods. 2009, 15(4): 697-706.
Leung Y.L., Tsui Y.K., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 55th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA. 2010.
Tsui Y.K., Leung Y.L., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Tsui Y.K., Cheung K.M.C., Leung Y.L., Chan D. and Kao R.Y.T., Uncovering new compounds for treatment of interveretebral disc degeneration by chemical genetics, 2010.


Researcher : Tsui YK

List of Research Outputs

Cheng H.W., Tsui Y.K., Cheung K.M.C., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres - a 3D model to study the effects of acellular matrix on stem cell fate, Tissue Engineering Part C Methods . 2009, 15(4): 697-706.
Cheng H.W., Tsui Y.K., Cheung K.M.C., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres. , WACBE (World Association for Chinese Biomedical Engineers) World Congress on Bioengineering 2009. 26-29 Jul 2009, Hong Kong, China. . 2009, 58.
Cheng H.W., Tsui Y.K., Cheung K.M., Chan D. and Chan B.P., Decellularization of chondrocyte-encapsulated collagen microspheres: a three-dimensional model to study the effects of acellular matrix on stem cell fate , Tissue Engineering, Part C Methods. 2009, 15(4): 697-706.
Leung Y.L., Tsui Y.K., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 55th Annual Meeting of the Orthopaedic Research Society, New Orleans, USA. 2010.
Tsui Y.K., Leung Y.L., Kao R.Y.T., Masuda K., Chan D. and Cheung K.M.C., Identifying therapeutic chemical agents for intervertebral disc degeneration by high throughput screening, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Tsui Y.K., Cheung K.M.C., Leung Y.L., Chan D. and Kao R.Y.T., Uncovering new compounds for treatment of interveretebral disc degeneration by chemical genetics, 2010.


Researcher : Vidyadhara S

List of Research Outputs

Luk K.D.K., Vidyadhara S., Lu D.S., Wong Y.W., Cheung W.Y. and Cheung K.M.C., Coupling between sagittal and frontal plane deformity correction in idiopathic thoracic scoliosis and its relationship with post-operative sagittal alignment, Spine. 2010, 35(11): 1158-1164.


Researcher : Wang Q

List of Research Outputs

Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., Posterior dislocation after total hip replacement - the effect of position of acetabular shell and hip ball size, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S33.
Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., 全髋關節置換術後的前脫位- 骨盆旋轉和股骨頭大小的影響, The Eleventh National Congress Of Orthopaedic Surgery And The Fourth International Congress Of Chinese Orthopaedic Association. 2009, 64-65.


Researcher : Wang T

List of Research Outputs

Liang G.L., Yang Z.M., Zhang R.J., Li L.H., Fan Y.J., Kuang Y., Gao Y., Wang T., Lu W.W. and Xu B., Supramolecular Hydrogel of a D-Amino Acid Dipeptide for Controlled Drug Release in Vivo , LANGMUIR. 2009, 25(15): 8419-8422.
Luk K.D.K., Lu W.W., Zhou G., Peng S.L., Wang T. and Li Z., New Investigator Recognition Award., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010. 2010.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Wang T

List of Research Outputs

Liang G.L., Yang Z.M., Zhang R.J., Li L.H., Fan Y.J., Kuang Y., Gao Y., Wang T., Lu W.W. and Xu B., Supramolecular Hydrogel of a D-Amino Acid Dipeptide for Controlled Drug Release in Vivo , LANGMUIR. 2009, 25(15): 8419-8422.
Luk K.D.K., Lu W.W., Zhou G., Peng S.L., Wang T. and Li Z., New Investigator Recognition Award., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010. 2010.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Wen C

List of Research Outputs

Cui J., Wen C., Hu Y. and Luk K.D.K., Precise Diagnosis of Cervical Spondylotic Myelopathy based on Quantitative Fiber Tractography, The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Deng L.I.A.N.-.F.U., Wen C. and Lu W.W., Orthopaedic Research in China: Prospective Review, 我国骨科基础科学研究的现状与未来发展方向, Orthopedic Journal of China . 中国矫形外科杂志, 2009.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Multiple Bioimaging Modalities Application for Chronic Compressive Spinal Cord of Rat. , the 54th Annual Meeting of the Orthopedic Research Society. New Orleans, Louisiana; 2010.
Hu Y., Wen C., Li T.H., Wu E.X. and Luk K.D.K., Ultrastructural imaging of Cervical Spinal Cord with Induced Compressive Injuries in a Rat Model., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010.. 2010.
Long H.O.U.-.Q.I.N.G., Wen C., Hu Y., Liu S.H.A.O.-.Y.U. and Li F.U.-.B.A.O., The Establishment Of The Experimental Chronic Compressive Myelopathy And The Mechanism Of Somatosensory-evoked Potential's Evaluation , 慢性压迫性脊髓症研究平台的建立及体感诱发电位功能评价的机制, Chinese Journal of Orthopedics. 中华骨科杂志, 2010.
Qin L.I.N.G., Wang L.I.N., Wong M.A.R.G.R.E.T. .W., Wen C., Wang G.A.N.G., Zhang G.E., Chan K.A.I.M.I.N.G., Cheung W.H. and Leung K.S., Osteogenesis induced by extracorporeal shockwave in treatment of delayed osteotendinous junction healing, Journal of Orthopaedic Research. 2010, 28: 70-6.
Wen C., Qin L., Lee K.M., Wong M.W. and Chan K.M., Grafted tendon healing in tibial tunnel is inferior to healing in femoral tunnel after anterior cruciate ligament reconstruction: a histomorphometric study in rabbits., Arthroscopy: the Journal of Arthroscopic and Related Surgery. 2010.
Wen C., Qin L., Lee K.M., Wong M.W. and Chan K.M., Influence of bone adaptation on tendon-to-bone healing in bone tunnel after anterior cruciate ligament reconstruction in a rabbit model., Journal Of Orthopaedic Research. 2009.
Wen C., Cui J., Hu Y. and Luk K.D.K., Loss of Entropy in Fractional Anisotropy Mapping of Spinal Cord in Patients of Cervical Spondylotic Myelopathy., The 26th CSRS ANNUAL MEETING, CORFU ISLAND, GREECE, 26-29 MAY 2010. . 2010.
Wen C., Hu Y., Li T.H., Lam K.O., Wu E.X. and Luk K.D.K., Multiple bio-imaging evaluations of spinal cord chronic compression in rat, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Wen C., Qin L., Lee K.M. and Chan K.M., Peri-graft bone mass and connectivity as predictors for the strength of tendon-to-bone attachment after anterior cruciate ligament reconstruction., Bone. 2009.


Researcher : Wong CT

List of Research Outputs

Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., 252. L-Asparagine on strontium hydroxyapatite nanorods morphology and biocompatibility , International Journal of Biomedical Engineering and Technology. 2009, 2(4): 303-318.
Lam R.W.M., Pan H., Li Z., Chen Y., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Fatty acid affects the nucleation of strontium-substituted biominerals, Ceram Int. 2010, 36(2): 683-688.
Lam R.W.M., Wong C.T., Li Z., Luk K.D.K., Cheung K.M.C., Chiu P.K.Y., Xu B., Chan W.K., Fang D., Leong J.C.Y. and Lu W.W., L-asparagine on strontium hyroxyapatite nanorods morphology and biocompatibility, International Journal Of Biomedical Engineering And Technology. 2009, 2: 303-318.
Lam W.M.R., Pan H., Li Z., Yang C., Chan W.K., Wong C.T., Luk K.D.K. and Lu W.W., Strontium-substituted calcium phosphates prepared by hydrothermal method under linoleic acid-ethanol solution, CERAMICS INTERNATIONAL. 2010, 36(2): 683-688.
Pan H., Li Z., Wang T., Lam R.W.M., Wong C.T., Darvell B.W., Luk K.D.K., Hu Y. and Lu W.W., Nucleation of strontium-substituted apatite, Crystal Growth & Design. 2009, 9: 33342-3345.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.


Researcher : Wong HM

List of Research Outputs

Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A Biodegradable Polymer-Based Coating To Control The Performance Of Magnesium Alloy Orthopaedic Implants, Biomaterials. 2010, 31(8): 2084-209.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., A New PCL Based Porous Membrane to Control the Degradation of Biodegradable Metallic Materials., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug–3Sep 2009. Poster presentation.. 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants biomaterials, Biomaterials. 2010, 31: 2084-2096.
Wong H.M., Yeung K.W.K., Leung Y.L., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., In-vitro analysis of gene expressions of osteoblastic cells while exposing to various concentrations of magnesium ions, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., Reduction of the Corrosion Rate of Biodegradable Metallic Materials by Al2O3 PIII surface Treatment., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24pril2010.. 2010, 868.


Researcher : Wong HM

List of Research Outputs

Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A Biodegradable Polymer-Based Coating To Control The Performance Of Magnesium Alloy Orthopaedic Implants, Biomaterials. 2010, 31(8): 2084-209.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., A New PCL Based Porous Membrane to Control the Degradation of Biodegradable Metallic Materials., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug–3Sep 2009. Poster presentation.. 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants biomaterials, Biomaterials. 2010, 31: 2084-2096.
Wong H.M., Yeung K.W.K., Leung Y.L., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., In-vitro analysis of gene expressions of osteoblastic cells while exposing to various concentrations of magnesium ions, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., Reduction of the Corrosion Rate of Biodegradable Metallic Materials by Al2O3 PIII surface Treatment., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24pril2010.. 2010, 868.


Researcher : Wong KL

List of Research Outputs

Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.


Researcher : Wong KL

List of Research Outputs

Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam R.W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., 254. Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. 2009, 30: 3810-3817.
Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M.R., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K., Lu W.W., Wong K.L., Wong C.T., Liu W.C., Pan H., Fong M.K., Lam W.M., Cheung W.L., Tang W.M., Chiu P.K.Y., Luk K.D.K. and Lu W.W., Mechanical properties and in vitro response of strontium-containing hydroxyapatite/polyetheretherketone composites, Biomaterials. Elsevier Ltd, 2009, 30: 3810-3817.


Researcher : Wong YL

List of Research Outputs

Hu Y., Wong Y.L., Lu W.W. and Kawchuk G.N., Creation of an asymmetrical gradient of back muscle activity and spinal stiffness during asymmetrical hip extension., Clin Biomech. 2009, 24: 799-806.


Researcher : Wong YW

List of Research Outputs

Chang K.C., Samartzis D., Fuego S.M., Dhatt S.S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Chang K.C., Samartzis D., Fuego S.M., Dhatt S., Wong Y.W., Cheung W.Y., Luk K.D.K. and Cheung K.M.C., The role of C1 posterior arch excision upon fusion in atlantoaxial arthrodesis with C1-C2 transarticular screws fixation, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in scoliosis correction with alternate level pedicle screw fixation, Journal of Bone and Joint Surgery. 2010, 92: 169-176.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation., 16th International Meeting on Advanced Spine Techniques, Vienna, Austria, July 15-18, 2009.. 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, 16th International Meeting on Advanced Spine Techniques (IMAST). Vienna, Austria, 2009.
Cheung K.M.C., Natarajan D., Samartzis D., Wong Y.W., Cheung W.Y. and Luk K.D.K., Predictability of the fulcrum bending radiograph in thoracic adolescent idiopathic scoliosis correction with alternate level pedicle screw fixation, J Bone Joint Surg (Am). 2010, 92: 169-76.
Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., In-vivo demonstration of spontaneous vertebral derotation in thoracic adolescent idiopathic scoliosis with fulcrum bending radiograph, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Kwan K., Samartzis D., Natarajan D., Cheung W.Y., Li J., Wong Y.W., Lenke L.G., Luk K.D.K. and Cheung K.M.C., Spine Chapter's Most Promising Spine Paper Award. Pedicle screw fixation strategies of the thoracic curve in adolescent idiopathic scoliosis., 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009. 2009.
Leung Y., Natarajan D., Samartzis D., Borkhuu B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis (AIS) using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Leung Y.L., Natarajan D., Samartzis D., Borkhuu B.B., Cheung W.Y., Wong Y.W., Cheung K.M.C. and Luk K.D.K., Selection of fusion levels in adolescent idiopathic scoliosis using the fulcrum bending radiograph prediction: verification based on pedicle screw strategy, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Luk K.D.K., Wan T., Wong Y.W., Cheung K.M.C., Chan Y.K., Cheng A., Kwan M., Law K., Lee P.W.H. and Cheing G.L.Y., A multidisciplinary functional restoration program for patients with chronic back pain, International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Luk K.D.K., Vidyadhara S., Lu D.S., Wong Y.W., Cheung W.Y. and Cheung K.M.C., Coupling between sagittal and frontal plane deformity correction in idiopathic thoracic scoliosis and its relationship with post-operative sagittal alignment, Spine. 2010, 35(11): 1158-1164.
Ming D., Bai Y., Liu X., Qi H., Cheng L., Wan B., Hu Y., Wong Y.W., Luk K.D.K. and Leong J.C.Y., A gait stability investigation into FES-assisted paraplegic walking based on the walker tipping index. , J Neural Eng. 2009, 6: 6600-7.
Ming D., Hu Y., Wong Y.W., Wan B.K., Luk K.D.K. and Leong J.C.Y., Risk-tendency graph (RTG): a new gait-analysis technique for monitoring FES-assisted paraplegic walking stability., Med Sci Monit. . 2009, 15: 105-12.
Wong Y.W., Cheung K.M.C., Cheung W.Y. and Luk K.D.K., The importance of spinal cord monitoring before and after turning the patients prone for operations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of ninety-one cases, Spine. 2010, 35(6): 635-641.
Yee D., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, Spine. 2010, 35: 635-641.


Researcher : Xiao J

List of Research Outputs

Lam S.K.L., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Luk K.D.K., The effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., International Society for the Study of the Lumbar Spine Annual Meeting, Auckland, New Zealand, April 13-17, 2010.
Luk K.D.K., Xiao J., Ruan D.K., Ding Y., Lu W.W. and Lam S.K.L., Effects of mal-placement of intervertebral disc allograft transplant on the kinematics of the spine., 2010 Annual Meeting of the American Academy of Orthopaedic Surgeons, New Orleans, Louisiana, March 9-13, 2010.


Researcher : Yan CH

Project Title:The genetic association between TRP2 allele of COL9A2 and knee osteoarthritis
Investigator(s):Yan CH, Chiu PKY, Cheung KMC, Chan D
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:11/2009
Abstract:
Osteoarthritis (OA) is one of most common skeletal diseases characterized by the progressive loss of articular cartilage in synovial joints. OA is also the most common cause of the limitation of activities of daily living after middle age. The prevalence of self-reported knee pain in the UK adults at least 40 years of age is between 20-28%, with approximately 50% reporting disability as a result of their knee pain. In the Framingham OA knee study showed of Caucasian subjects over 60 years of age, the radiographic prevalence was 34.4% in women and 30.9% in men; the symptomatic prevalence 11.4%, and 6.8%, respectively [1]. Recent studies in China showed that the incidence of radiographic and symptomatic knee OA is higher in Chinese women compared to their Caucasian counterparts. The Beijing OA knee study showed that the radiographic and symptomatic prevalence in elderly women was 42.8% and 15% respectively [2]. OA is characterized by both degenerative and reactive changes in the articular cartilage and subchondral bone, thought to be driven by a spectrum of environmental and genetic factors operating via both biomechanical and biochemical mechanisms. The changes in articular cartilage include the progressive loss of extracellular matrix (ECM) and collagen breakdown due to a combination of increased catabolism and decreased synthesis. In the aetiology of OA, human genetic approaches are the most valuable tools. Genetic variations are nature’s experiment on humans, and the investigating such variation brings the greatest insight into chronic disease like OA. The genetic association study is a simple two-step procedure, much like arresting and convicting a criminal. First we investigate our suspect - a sequence variation in a gene of interest, and then collect the evidence in the form of DNA – the sequence variations’ genotype distribution in affected patients (cases) and unaffected controls. If we can find an association between the crime (disease) and the suspect (candidate gene), we can convict the criminal (culprit gene). The articular cartilage consists primarily of extra-cellular matrix, with a sparse population of cells. The matrix consists of 2 components: tissue fluid and structural macromolecules. The latter includes collagens, proteoglycans and noncollagenous proteins which contribute 20% to 40% of the wet weight of the tissue. The major collagen type is II (90% - 95%), with small amounts of type VI, IX, X and XI collagens. Collagen IX is found throughout all cartilage layers in healthy and OA tissue. Koelling et al. [3] found that cells deposited collagen IX in the pericellular and inter-territorial matrix and a 66% higher amount of collagen IX was detected in the pericellular matrix of the weight bearing areas adjacent to the main defect in comparison to the intact area in knee joints. There was also 3.72 times higher amount of the respective mRNA. It might reflect an attempt on the part of the diseased cartilage to stabilize and protect the remaining matrix of late-stage osteoarthritis cartilage from further destruction. The co-investigators (KMC Cheung & D Chan) are involved in the Degenerative Disc Disease (DDD) study, as a part of the Area of Excellence (AoE) programme in “Developmental genomics and skeletal research” established by the Hong Kong Government. This programme is a collaboration between scientists and clinicians from four Universities in Hong Kong (HKU, HKUST, PolyU and OpenU) combining diverse and complementary expertise. The team employs a variety of state-of-the-art technologies in genomics, proteomics, cell biology and animal models to identify the genes, proteins and regulatory networks that are responsible for regulating chondrocyte differentiation and for maintaining the integrity of the skeleton. Part of the goals is to identify genetic risk factors in DDD. Several genetic risk factors have been identified by using candidate gene approach (Song et al., 2008; Iita et al., 2007; Yeun et al., 2007; Cheung et al., 2006; Jim et al., 2005) and a major locus was detected in early onset familial DDD patients (Ho et al., 2007). More recently, a small scale genome-wide association studies (GWAS) of a 400 DDD sample set was performed (Kao et al., 2008). Borrowing the experience from the DDD study, we aim to establish a genetic database on OA knee that will contribute to the understanding and management of the disease, and as an independent cohort to identify new risk factors as as well validating previous findings, as replication of association findings in different cohort is the gold standard for a ‘true’ association. The human lumbar intervertebral disc (IVD) is a complex fibro-cartilaginous structure consisting of three distinct components: 1) the nucleus pulposus; 2) the annulus fibrosus; and 3) the cartilaginous endplates, all of which are rich in proteoglycan and collagen. With many similarities in the molecular composition of the articular cartilage and IVD, it is therefore logical to suggest that OA knee and disc DDD of lumbar spine might share some common risk factors that included some collagen genes, vitamin D receptor, cartilage intermediate layer protein, asporin, and matrix metalloproteinases [4] [5]. The traditional view is that the factors associated with both diseases are related to age, gender, occupation and cigarette smoking etc. With the increase in understanding of the genetics contribution, we can begin to address the role of genetic factors in the development of these two degenerative diseases. Finnish studies found as association between low back pain and sciatica with 2 variants of the α-chain of collagen IX, encoded by Trp2 and Trp3 alleles. Trp2 was only found in affected individuals (4%) [6] [7]. Our group, in one of the largest known studies for DDD and genetic predisposition, demonstrated an association between the two alleles and lumbar disc degeneration in Southern Chinese volunteers. We found the Trp 2 allele was present in 20% of the Hong Kong population and was associated with an increase in the risk of developing annular tear, disc degeneration and disc herniation. Affected Trp2 individuals had more severe degeneration, and that the mechanical properties of Trp2 intervertebral discs are impaired [8]. So far there is no published literature on the association between the Trp2 allele and knee osteoarthritis. Our hypothesis is Trp2 allele polymorphism is associated with OA knee in Southern Chinese subjects. Thus the aim of our study is to demonstrate such association. Our project will be the first one to investigate this possible link. Trp2 was showed to have high frequency in the local population (20%) [8], therefore the chance of establishing such association is high.


List of Research Outputs

Kuong E., Ng F.Y., Yan C.H., Chiu P.K.Y. and Fang C., Antibiotic Prophylaxis After Total Joing Replacements, Hong Kong Medical Journal. 2009, 15: 458-62.
Ng F.Y., Chiu P.K.Y. and Yan C.H., Anterior Dislocation After Total Hip Replacement - Effects Of Pelvic Rotation And Hip Ball Size, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Ng F.Y., Jiang J.T., Chiu P.K.Y., Yan C.H. and Choi C.L., Anterior dislocation after total hip replacement - effects of pelvic rotation and hip ball size , The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S56.
Ng F.Y., Chiu P.K.Y., Yan C.H. and Ng K.F., Pain control after total knee replacement - comparison of local anaesthetics via continuous femoral catheter with patient-controlled analgesia, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S61.
Ng F.Y., Chiu P.K.Y. and Yan C.H., Posterior Dislocation After Total Hip Replacement - Effects Of Position Of Acetabular Shell And Hip Ball Size, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., Posterior dislocation after total hip replacement - the effect of position of acetabular shell and hip ball size, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S33.
Ng F.Y., Wang Q., Chiu P.K.Y. and Yan C.H., 全髋關節置換術後的前脫位- 骨盆旋轉和股骨頭大小的影響, The Eleventh National Congress Of Orthopaedic Surgery And The Fourth International Congress Of Chinese Orthopaedic Association. 2009, 64-65.
Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented Revision Total Hip Arthroplasty Using Impaction Bone Grafting And Elite Plus Stem: A Retrospective Review Of 15 Cases, 29th Annual Congress Of The Hong Kong Orthopaedic Association. 2009.
Yan C.H., Ng T.P., Chiu P.K.Y. and Ng F.Y., Cemented revision total hip arthroplasty using impaction bone grafting and Elite plus stem: A retrospective review of 15 cases, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S58.
Yan C.H., Chiu P.K.Y. and Ng F.Y., QMH Experience in Primary TKR for Primary Knee Osteoarthritis 2000 - 2009, 7th Hong Kong International Orthopaedic Forum. 2010.
Yan C.H., Chiu P.K.Y. and Ng F.Y., 36毫米股骨頭在國人全髋置換中之效果, The Eleventh National Congress Orthopaedic Surgery And The Fourth International Congress Of Chinese Orthopaedic Association. 2009, 57.
Yan C.H., The Musculo-skeletal System, The Hong Kong Association Of The Pharmaceutical Industry Training. 2009.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The Radiographic Prevalence Of Knee Osteoarthritis In Local Chinese Subjects: A Retrospective Review Of 102 Angiogram Images, 29th Annual Congress Of The Hong Kong Orthopaedics Association. 2009.
Yan C.H., Chiu P.K.Y., Ng T.P. and Ng F.Y., The radiographic prevalence of knee osteoarthritis in local Chinese subjects: a retrospective review of 102 angiogram images, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S56.
Yan C.H. and Chiu P.K.Y., Total Hip Arthroplasty For Failed Internal Fixation Of Inter-trochanteric Fractures, 11th Asia-pacific Arthroplasty Society Annual Scientific Meeting. 2009.
Yan C.H., Chiu P.K.Y. and Ng F.Y., Total Hip Arthroplasty with 36mm Articulation in Chinese Patients, Annual Congress of Hong Kong Orthopaedic Association. 2009.
Yau W.P., Chiu P.K.Y. and Yan C.H., Cutting errors in Total Knee Replacement, 10th Annual Meeting of Computer Assisted Orthopaedic Surgery – International. 2010.


Researcher : Yang F

List of Research Outputs

Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., 250. Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009, 17: 1959-1966.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009.


Researcher : Yang F

List of Research Outputs

Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., 250. Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009, 17: 1959-1966.
Yang F., Leung Y.L., Luk K.D.K., Chan D. and Cheung K.M.C., Mesenchymal stem cells arrest intervertebral disc degeneration through chondrocytic differentiation and stimulation of endogenous cells, Molecular Therapy. 2009.


Researcher : Yau PWP

Project Title:Development of Novel Plasma Modified Metallic Materials for Anterior Cruciate Ligament Reconstruction
Investigator(s):Yau WP
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2008
Abstract:
To develop a novel plasma modified metallic material for the use in Anterior Cruciate Ligament reconstruction. Seed funding is sought to perform preliminary plasma modified metallic materials sample preparation and optimization; and development of a new small animal model (rabbit model) utilizing double fixation technique in Anterior Cruciate Ligament Reconstruction for future testing of the newly developed biomaterial. Anterior Cruciate Ligament (ACL) Rupture is one of the commonest injuries in Sports Medicine. The mainstay of treatment is Arthroscopic assisted intra-articular ACL Reconstruction. Soft tissue tendon graft (e.g. hamstring graft) is commonly employed because of low donor site morbidity and high initial tensile strength. It is estimated that there are more than 100,000 anterior cruciate ligament reconstructions performed in United States each year [1]. Despite the prevalence of ACL reconstruction surgery, method in achieving prompt and reproducible osteointegration of the graft within the bone tunnel is still a significant issue to address. Results from a number of animal studies show that early graft healing in terms of Sharpey-like fiber formation takes place only at 12 weeks after the index procedure [2-7]. A common cause for failed ACL reconstruction is a failure of the graft-to-bone healing [8]. It is estimated that there are around 3,000 to 10,000 revision ACL reconstructions in United States per annum [1], accounting for 3 – 10% of the total number of ACL reconstruction surgery. The initial stability of the graft-bone interface is secured by the use of orthopaedic implants. This can be in the form of suspensory device (e.g. EndoButton* CL, Smith and Nephew) or aperture fixation device such as interference screw (e.g. Softsilk* 1.5, Smith and Nephew). There is a concern regarding graft-tunnel micromotion in suspensory device, which leads to a worry in the reproducibility of graft-host incorporation [8]. Thus, the aperture device (in terms of the interference screw fixation) is still the commonest method used in fixing graft material in ACL reconstruction. The aperture device (interference screws) works by friction. The initial pull out strength is satisfactory [9]. However, there is a concern on the loss of initial fixation strength due to thermonecrosis and bone resportion, leading to implant migration in aperture fixation method [10]. Beside, the use of implant in aperture fixation method will decrease the effective surface area available for graft-host incorporation [8]. This can lead to a suboptimal healing in the graft-bone interface and subsequently increasing the possibility of failure and hence revision surgery. Thus, there is a need of developing an implant which provides adequate stability to the graft-tunnel interface before graft-bone incorporation occurs. There was an attempt in improving the fixation of interference screw using hydroxyapatite and tricalcium phosphate coating. However, the hypothesis of an improved graft-tunnel stability using this type of surface treatment was not supported by clinical study [11]. We plan to use plasma immersion ion implantation (PIII) to modify and enhance the bioactivity of implantable metallic materials such as titanium alloys, which is one of the commonly used materials in orthopaedic implant in ACL reconstruction. This technology can directly modify the surface chemistry and topography together with mechanical properties of the materials by implanting different elements into the surface region. We have shown in our previous study in improving the bioactivity of titanium alloy by demonstrating superior adhesion and bone growth after hydrogen and water plasma implantation [12]. In addition, our team also demonstrates the alteration of surface topography of nickel titanium alloy in nano scale by applying various implantation voltages. Our results suggest that the ripple surface in nano scale favors osteoblast proliferation as compared with the untreated sample [13, 14]. The success of applying this technology in interference screw will lead to a rapid, reproducible, stable interface between the implant and the host bone. We plan to further use this technology (PIII) to enhance fibroblast proliferation at the implant-graft interface. The aim is to encourage Sharpley-like fiber formation also in the implant-graft interface, allowing the graft not only having biological integration with the host bone, but also integration with the implant. This is expected to result in reduction in the failure of the ACL reconstruction. In order to test the biointegradability of the newly developed PIII modified titanium implants, we plan to develop our own small animal model (rabbit) using double fixation technique. Most of the small animal models concerning ACL reconstruction experiment in the literature utilize only suspensory fixation method [3, 15]. This is not adequate for testing of possible biointegration of the implants placed in the bone tunnel. Thus, we need to develop our own animal model for future testing of the biointegration of both the graft and the implants. The primary objective of the proposed project is: (1) to develop a novel plasma modified metallic materials for the use in Anterior Cruciate Ligament reconstruction. The secondary objective is: (2) to develop a small animal model of ACL reconstruction using double fixation technique for comparing the performance of the newly developed novel biomaterials with the traditional titanium implant.


Project Title:Use of Strontium in enhancing Tendon Osteointegration in a Rabbit Anterior Cruciate Ligament Reconstruction Model
Investigator(s):Yau WP, Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:10/2008
Completion Date:09/2010
Abstract:
Objective: To test the possibility of using Strontium-containing Hydroxyapatite (Sr-HA) bioactive bone cement in accelerating osteointegradation of soft tissue tendon graft in a New Zealand Rabbit Anterior Cruciate Ligament reconstruction model. Seed funding is sought to perform preliminary small animal studies in order to demonstrate the superiority of Sr-HA over the traditional allograft reconstruction and recently experimented Tricalcium phosphate enhancement technique. Anterior Cruciate Ligament (ACL) Rupture is one of the commonest injuries in Sports Medicine. The mainstay of treatment is Arthroscopic assisted intra-articular ACL Reconstruction. Soft tissue tendon graft (e.g. hamstring graft) is commonly employed because of low donor site morbidity and high initial tensile strength. It is estimated that there are more than 100,000 anterior cruciate ligament reconstructions performed in United States each year [1]. Despite the prevalence of ACL reconstruction surgery, method in achieving prompt and reproducible osteointegration of the graft within the bone tunnel is still a significant issue to address. Results from a number of animal studies show that early graft healing in terms of Sharpey-like fiber formation takes place only at 12 weeks after the index procedure [2-7]. It is a common practice to protect the graft-host interface before osteointegration occurs. Patients are required to abstain from pivoting activity for at least six to nine months. Otherwise, instability may recur. Despite a reported initial success rate of 60% to 90% after ACL Reconstruction, it is estimated that there are around 3,000 to 10,000 revision ACL reconstructions in United States per annum [1]. Many surgeons believe that the problem of incorporation of the graft in the bone tunnel is one of the significant issues in leading to this problem. Recent attempts have been made in enhancing graft incorporation using stem cell technology [8] and osteoconductive substance (e.g. tricalcium phosphate) [2] in the graft-host junction. Results from preliminary animal studies are encouraging. The mesenchymal stem cell enhanced ACL reconstruction showed a mature zone of fibrocartilage blending from bone to the graft in eight weeks time. However, the need of an additional procedure for harvesting the mesenchymal stem cell made this technique less appealing. On the other hand, the use of osteoconductive substance (e.g. tricalcium phosphate) produced superior osteointegration than the control limb in animal study at three months time. However, osteointegration was observed still only in limited parts of the bone-tendon junction, with the majority of the interface having a Sharpey-like fiber link between the bone and the graft. We have developed a strontium-containing hydroxyapatite (Sr-HA) bioactive bone cement [9-13]. We have shown that this bioactive cement promotes osteoblasts attachment and mineralization in vitro and bone growth and osteointegration in vivo. Oral strontium has been shown to induce new bone formation. Our data suggest that strontium delivered locally has the same effect. We will like to introduce this strontium-containing hydroxyapatite bioactive bone cement as a novel substance to enhance osteointegration of soft tissue tendon graft in Anterior Cruciate Ligament Reconstruction. We hypothesize that the local release of strontium can lead to an accelerated osteointegration of soft tissue tendon graft within the bone tunnel in ACL Reconstruction. We plan to conduct short term small animal experiment to test this hypothesis. The aim is to accelerate the post-ACL reconstruction rehabilitation in short term and to reduce the number of revision surgery in long run. The primary objective of the proposed project is: (1) to study the biological response of soft tissue tendon allograft coated with Sr-HA bioactive bone cement in a New Zealand Rabit Anterior Cruciate Ligament Reconstruction model The secondary objective is: (2) to compare the different stages of osteointegration among Sr-HA treated allograft, Tricalcium phosphate treated allograft and control group (i.e. allograft without any surface treatment). References: 1. Gordon MD and Steiner ME. Anterior cruciate ligament injuries. In: Garrick JG, editor. Orthopaedic knowledge update. Sports medicine. Edition 3. Rosemont: American Academy of Orthopaedic Surgeons; 2004. p. 169-181. 2. Huangfu XQ and Zhao JZ. Tednon-bone healing enhancement using injectable tricalcium phosphate in a dog anterior cruciate ligament reconstruction model. Arthroscopy. 2007; 23(5):455-62. 3. Kohno T, Ishibashi Y, Tsuda E, Kusumi T, Tanaka M and Toh S. Immunohistochemical demonstration of growth factors at the tendon-bone interface in anterior cruciate ligament reconstruction using a rabbit model. Journal of Orthopaedic Science. 2007; 12:67-73. 4. Yoshikawa T, Tohyama H, Katsura T, Kondo E, Kotani Y, Matsumoto H, et al. Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep. American Journal of Sports Medicine. 2006; 34(12): 1918-25. 5. Goradia VK, Rochat MC, Kida M and Grana WA. Natural history of a hamstring tendon autograft used for anterior cruciate ligament reconstruction in a sheep model. American Journal of Sports Medicine. 2000; 28(1): 40-6. 6. Goradia VK, Rochat MC, Grana WA, Rohrer MD and Prasad HS. Tendon-to-bone healing of a semitendinosus tendon autograft used for ACL reconstruction in a sheep model. American Journal of Knee Surgery. 2000; 13:143-51. 7. Weiler A, Hoffmann RFG, Bail HJ, Rehm O and Sudkamp NP. Tendon healing in a bone tunnel. Part II: histological analysis after biodegradable interference fit fixation in a model of anterior cruciate ligament reconstruction in sheep. Arthroscopy. 2002; 18(2): 124-35. 8. Soon MYH, Hassan A, Hui JHP, Goh JCH and Lee EH. An analysis of soft tissue allograft anterior cruciate ligament reconstruction in a rabbit model. A short-term study of the use of mesenchymal stem cells to enhance tendon osteointegration. American Journal of Sports Medicine. 2007; 35(6): 962-71. 9. Cheung KMC, Lu WW, Luk KDK, Wong CT, Chan D, Shen JX, et al. Vertebroplasty by use of a strontium-containing bioactive bone cement. Spine. 2005; 30(17S): S84-91. 10. Wong CT, Lu WW, Chan WK, Cheung KMC, Luk KDK, Lu DS, et al. In vivo cancellous bone remodeling on a strontium-containing hydroxyapatite (Sr-HA) bioactive cement. J Biomed Mater and Res. 2004; 68A:513-21. 11. Lu WW, Cheung KMC, Li YW, Luk KDK, Holmes AD, Zhu QA, et al. Bioactive bone cement as a principal fixture for spinal burst fracture: an in vitro biomechanical and morphologic study. Spine. 2001; 26(24):2684-91. 12. Wong CT, Chen QZ, Lu WW, Leong JCY, Chan WK, Cheung KMC, et al. Ultrastructural study of mineralization of a strontium-containing hydroxyapatite (Sr-HA) cement in vivo. J Biomed Mater and Res. 2004; 70:428-35. 13. Li YW, Leong JCY, Lu WW, Luk KDK, Cheung KMC, Chiu KY, et al. A novel injectable bioactive bone cement for spinal surgery: a developmental and preclinical study. J Biomed Mater and Res. 2000; 52:164-70.


Project Title:Development of a large animal knee osteoarthritis model using high tibial varus osteotomy in sheep
Investigator(s):Yau WP, Chiu PKY, Lie CWH
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2009
Abstract:
Objective: The primary objective is to develop a large animal model of knee osteoarthritis (OA) with varus lower limb malalignment using high tibial varus osteotomy in sheep. The secondary objective is to evaluate the relationship between the different types of articular cartilage assessment between OA model induced by extra-articular (High tibial varus osteotomy) and intra-articular method (medial meniscectomy). Osteoarthritis of knee is the most common form of knee arthritis and a leading cause of total knee arthroplasty. It is estimated that there are around 140,000 total knee replacements performed in United States each year (1). As a leading joint replacement center in Hong Kong, we observe that there is a relatively higher incidence of total knee replacement in Hong Kong when compared with the Caucasian statistics. We also observed that there was a higher degree of coronal and sagittal plane bowing of both femur and tibia (2-3) and more severe varus malalignment in coronal plane in Chinese population receiving total knee arthroplasty (4). This observation was echoed by similar studies reported in other oriental population, including Japan (5). We had studied the osteometry of lower limb in fifty asymptomatic young skeletally mature volunteers. We found that there was a statistically significant more varus alignment of lower limb and varus inclination of the joint line in the coronal plane in Chinese when compared with similar Caucasian report (6). Varus malalignment of the lower limb is important in the development and progression of osteoarthritis of knee (7-8). During activities of daily living, the medial compartment is loaded about 50% more than the lateral compartment due to the adduction moment produced during weight bearing (9–11). Varus malalignment shifts the load bearing axis to the medial which further increases the total load passing the medial compartment during gait (12). A vicious cycle will continue and lead to further progression of medial compartment OA. The observation of a more varus lower limb alignment in asymptomatic young skeletally mature Chinese volunteers (6) leads us to believe that the increased incidence of end stage osteoarthritis of knee joint observed in Chinese population may be partially related to this mechanical disadvantage in the osteometry of lower limb (i.e. varus lower limb malalignment), which is present after skeletal maturity in Chinese. However, the available knee osteoarthritis models in animal fail to address the significance of this mechanical disadvantage in production of knee joint osteoarthritis. The current available knee osteoarthritis animal model include [1] spontaneous model in aging animals, [2] genetically modified mice, [3) surgically induced (including meniscectomy and Anterior cruciate ligament deficient knee model), [4] enzymatically induced and [5] chemically induced model (13-15). Spontaneous models best mimic the slow progression of the human disease but they are time consuming and progression is variable (16). Genetically modified mice constitute the best tools for understanding the functional role of specific molecules in cartilage homeostasis and OA pathology. However, the size of the animal makes this model not a good representative of human condition, especially in weight bearing areas including the knee and hip joint (17). Surgically (18) and enzymatically induced models (19) develop rapid and reproducible damage, but might be more relevant to the traumatic forms of OA than to the classical degenerative form of OA. Chemically induced models develop even more rapid damage, but their physiological relevance is debatable (20). All of the above osteoarthritis animal models fail to address the contribution of the adverse mechanical lower limb environment (i.e. varus malalignment of lower limb in coronal plane) in the pathophysiology of osteoarthritis. In view of the prevalence of varus malalignment in the young healthy local Chinese population, the development of such a malalignment osteoarthritis model in a large animal setting is essential for us to understand the development of osteoarthritis in Chinese and to evaluate different type of potential interventional treatment in our local population. Thus, we propose to develop a new large animal model of knee osteoarthritis using varus high tibial osteotomy in Merino sheep to reproduce the adverse mechanical malalignment that is observed in Chinese population. Reference: 1. Mahomed: Epidemiology of Total Knee Replacements in United States. Medicare Population. Journal Bone and Joint Surgery, 2005; 87A:1222-8. 2. Yau WP: Coronal Bowing of the Femur and Tibia in Chinese – Its incidence and effect in total knee arthroplasty planning. J Orthop Surg 2007; 15:32-6. 3. Tang WM: Sagittal bowing in distal femur and total knee arthroplasty. J Orthop Research 2005, 23:41-5. 4. WP Yau: Computer navigation did not improve alignment in a lower-volume total knee practice. Clinical Orthopaedics and Related Research 2008, 466(4):935-45. 5. Nagamine: Anatomical variations should be considered in total knee arthroplasty. J Orthop Science 2000, 5:232-7. 6. WM Tang: Axial alignment of the lower extremity in Chinese Adults. J Bone Joint Surg 2000, 82A, 1603-8. 7. Brouwer: Association between valgus and varus alignment and the development and progression of radiographic osteoarthritis of the knee. Arthritis Rheum 2007; 1204-11. 8. Sharma: The role of knee alignment in disease progression and functional decline in knee osteoarthritis. JAMA 2001; 286:188-95. 9. Andriachhi: Dynamics of knee malalignment. Orthop Chin North Am 1994; 25: 395-403. 10. Morrison: The mechanics of the knee joint in relation to normal walking. J Biomech 1970; 3: 51-56. 11. Moskowitz: Osteoarthritis 4th edition: Ch 21B: p.395-414. 12. Hsu RW: Normal axial alignment of the lower extremity and load-bearing distribution at the knee. Clin Orthop 1990; 255: 215-27. 13. Bendele: Animal models of osteoarthritis. J Musculoskelet Neuronal Interact 2001; 1: 363-376. 14. Van den Berg WB: Lessons from animal models of osteoarthritis. Curr Opin Rheumatol 2001; 13: 452-456. 15. Ameye: Animal models of osteoarthritis: lessons learned while seeking the “Holy Grail” Curr Opin Rheumatol 2006, 18:537-47. 16. Jallali: Vulnerability to ROS-induced cell death in ageing articular cartilage: analysis of cell density, apoptosis-related gene expression and mechanism controlling chondrocyte apoptosis. Osteoarthritis Cartilage 2004, 12:917-23. 17. Glasson: Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature 2005, 434:644-8. 18. Quasnichka: Cruciate ligament laxity and femoral intercondylar notch narrowing in early-stage knee osteoarthritis. Arthritis Rheum 2005, 52:3100-9. 19. Blom: Synovial lining macrophages mediate osteophyte formation during experimental osteoarthritis. Osteoarthritis Cartilage 2004, 12:627-35. 20. Bove: Weight bearing as a measure of disease progression nand efficacy of anti-inflammatory compounds in a model of monosodium iodoacetate-induced osteoarthritis. Osteoarthritis Cartilage 2003, 11:821-30.


Project Title:The Fourth International Congress of Chinese Orthopaedic Association Prospective Case Control Study comparing "Transportal"versus "Transtibial" technique in preparation of femoral tunnel in Arthroscopic Assisted Single bundle Hamstring Anterior Cruciate Ligament Reconstruction
Investigator(s):Yau WP
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:11/2009
Completion Date:11/2009
Abstract:
N/A


List of Research Outputs



Researcher : Yau WP

Project Title:Development of Novel Plasma Modified Metallic Materials for Anterior Cruciate Ligament Reconstruction
Investigator(s):Yau WP
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2008
Abstract:
To develop a novel plasma modified metallic material for the use in Anterior Cruciate Ligament reconstruction. Seed funding is sought to perform preliminary plasma modified metallic materials sample preparation and optimization; and development of a new small animal model (rabbit model) utilizing double fixation technique in Anterior Cruciate Ligament Reconstruction for future testing of the newly developed biomaterial. Anterior Cruciate Ligament (ACL) Rupture is one of the commonest injuries in Sports Medicine. The mainstay of treatment is Arthroscopic assisted intra-articular ACL Reconstruction. Soft tissue tendon graft (e.g. hamstring graft) is commonly employed because of low donor site morbidity and high initial tensile strength. It is estimated that there are more than 100,000 anterior cruciate ligament reconstructions performed in United States each year [1]. Despite the prevalence of ACL reconstruction surgery, method in achieving prompt and reproducible osteointegration of the graft within the bone tunnel is still a significant issue to address. Results from a number of animal studies show that early graft healing in terms of Sharpey-like fiber formation takes place only at 12 weeks after the index procedure [2-7]. A common cause for failed ACL reconstruction is a failure of the graft-to-bone healing [8]. It is estimated that there are around 3,000 to 10,000 revision ACL reconstructions in United States per annum [1], accounting for 3 – 10% of the total number of ACL reconstruction surgery. The initial stability of the graft-bone interface is secured by the use of orthopaedic implants. This can be in the form of suspensory device (e.g. EndoButton* CL, Smith and Nephew) or aperture fixation device such as interference screw (e.g. Softsilk* 1.5, Smith and Nephew). There is a concern regarding graft-tunnel micromotion in suspensory device, which leads to a worry in the reproducibility of graft-host incorporation [8]. Thus, the aperture device (in terms of the interference screw fixation) is still the commonest method used in fixing graft material in ACL reconstruction. The aperture device (interference screws) works by friction. The initial pull out strength is satisfactory [9]. However, there is a concern on the loss of initial fixation strength due to thermonecrosis and bone resportion, leading to implant migration in aperture fixation method [10]. Beside, the use of implant in aperture fixation method will decrease the effective surface area available for graft-host incorporation [8]. This can lead to a suboptimal healing in the graft-bone interface and subsequently increasing the possibility of failure and hence revision surgery. Thus, there is a need of developing an implant which provides adequate stability to the graft-tunnel interface before graft-bone incorporation occurs. There was an attempt in improving the fixation of interference screw using hydroxyapatite and tricalcium phosphate coating. However, the hypothesis of an improved graft-tunnel stability using this type of surface treatment was not supported by clinical study [11]. We plan to use plasma immersion ion implantation (PIII) to modify and enhance the bioactivity of implantable metallic materials such as titanium alloys, which is one of the commonly used materials in orthopaedic implant in ACL reconstruction. This technology can directly modify the surface chemistry and topography together with mechanical properties of the materials by implanting different elements into the surface region. We have shown in our previous study in improving the bioactivity of titanium alloy by demonstrating superior adhesion and bone growth after hydrogen and water plasma implantation [12]. In addition, our team also demonstrates the alteration of surface topography of nickel titanium alloy in nano scale by applying various implantation voltages. Our results suggest that the ripple surface in nano scale favors osteoblast proliferation as compared with the untreated sample [13, 14]. The success of applying this technology in interference screw will lead to a rapid, reproducible, stable interface between the implant and the host bone. We plan to further use this technology (PIII) to enhance fibroblast proliferation at the implant-graft interface. The aim is to encourage Sharpley-like fiber formation also in the implant-graft interface, allowing the graft not only having biological integration with the host bone, but also integration with the implant. This is expected to result in reduction in the failure of the ACL reconstruction. In order to test the biointegradability of the newly developed PIII modified titanium implants, we plan to develop our own small animal model (rabbit) using double fixation technique. Most of the small animal models concerning ACL reconstruction experiment in the literature utilize only suspensory fixation method [3, 15]. This is not adequate for testing of possible biointegration of the implants placed in the bone tunnel. Thus, we need to develop our own animal model for future testing of the biointegration of both the graft and the implants. The primary objective of the proposed project is: (1) to develop a novel plasma modified metallic materials for the use in Anterior Cruciate Ligament reconstruction. The secondary objective is: (2) to develop a small animal model of ACL reconstruction using double fixation technique for comparing the performance of the newly developed novel biomaterials with the traditional titanium implant.


Project Title:Use of Strontium in enhancing Tendon Osteointegration in a Rabbit Anterior Cruciate Ligament Reconstruction Model
Investigator(s):Yau WP, Lu WW
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:10/2008
Completion Date:09/2010
Abstract:
Objective: To test the possibility of using Strontium-containing Hydroxyapatite (Sr-HA) bioactive bone cement in accelerating osteointegradation of soft tissue tendon graft in a New Zealand Rabbit Anterior Cruciate Ligament reconstruction model. Seed funding is sought to perform preliminary small animal studies in order to demonstrate the superiority of Sr-HA over the traditional allograft reconstruction and recently experimented Tricalcium phosphate enhancement technique. Anterior Cruciate Ligament (ACL) Rupture is one of the commonest injuries in Sports Medicine. The mainstay of treatment is Arthroscopic assisted intra-articular ACL Reconstruction. Soft tissue tendon graft (e.g. hamstring graft) is commonly employed because of low donor site morbidity and high initial tensile strength. It is estimated that there are more than 100,000 anterior cruciate ligament reconstructions performed in United States each year [1]. Despite the prevalence of ACL reconstruction surgery, method in achieving prompt and reproducible osteointegration of the graft within the bone tunnel is still a significant issue to address. Results from a number of animal studies show that early graft healing in terms of Sharpey-like fiber formation takes place only at 12 weeks after the index procedure [2-7]. It is a common practice to protect the graft-host interface before osteointegration occurs. Patients are required to abstain from pivoting activity for at least six to nine months. Otherwise, instability may recur. Despite a reported initial success rate of 60% to 90% after ACL Reconstruction, it is estimated that there are around 3,000 to 10,000 revision ACL reconstructions in United States per annum [1]. Many surgeons believe that the problem of incorporation of the graft in the bone tunnel is one of the significant issues in leading to this problem. Recent attempts have been made in enhancing graft incorporation using stem cell technology [8] and osteoconductive substance (e.g. tricalcium phosphate) [2] in the graft-host junction. Results from preliminary animal studies are encouraging. The mesenchymal stem cell enhanced ACL reconstruction showed a mature zone of fibrocartilage blending from bone to the graft in eight weeks time. However, the need of an additional procedure for harvesting the mesenchymal stem cell made this technique less appealing. On the other hand, the use of osteoconductive substance (e.g. tricalcium phosphate) produced superior osteointegration than the control limb in animal study at three months time. However, osteointegration was observed still only in limited parts of the bone-tendon junction, with the majority of the interface having a Sharpey-like fiber link between the bone and the graft. We have developed a strontium-containing hydroxyapatite (Sr-HA) bioactive bone cement [9-13]. We have shown that this bioactive cement promotes osteoblasts attachment and mineralization in vitro and bone growth and osteointegration in vivo. Oral strontium has been shown to induce new bone formation. Our data suggest that strontium delivered locally has the same effect. We will like to introduce this strontium-containing hydroxyapatite bioactive bone cement as a novel substance to enhance osteointegration of soft tissue tendon graft in Anterior Cruciate Ligament Reconstruction. We hypothesize that the local release of strontium can lead to an accelerated osteointegration of soft tissue tendon graft within the bone tunnel in ACL Reconstruction. We plan to conduct short term small animal experiment to test this hypothesis. The aim is to accelerate the post-ACL reconstruction rehabilitation in short term and to reduce the number of revision surgery in long run. The primary objective of the proposed project is: (1) to study the biological response of soft tissue tendon allograft coated with Sr-HA bioactive bone cement in a New Zealand Rabit Anterior Cruciate Ligament Reconstruction model The secondary objective is: (2) to compare the different stages of osteointegration among Sr-HA treated allograft, Tricalcium phosphate treated allograft and control group (i.e. allograft without any surface treatment). References: 1. Gordon MD and Steiner ME. Anterior cruciate ligament injuries. In: Garrick JG, editor. Orthopaedic knowledge update. Sports medicine. Edition 3. Rosemont: American Academy of Orthopaedic Surgeons; 2004. p. 169-181. 2. Huangfu XQ and Zhao JZ. Tednon-bone healing enhancement using injectable tricalcium phosphate in a dog anterior cruciate ligament reconstruction model. Arthroscopy. 2007; 23(5):455-62. 3. Kohno T, Ishibashi Y, Tsuda E, Kusumi T, Tanaka M and Toh S. Immunohistochemical demonstration of growth factors at the tendon-bone interface in anterior cruciate ligament reconstruction using a rabbit model. Journal of Orthopaedic Science. 2007; 12:67-73. 4. Yoshikawa T, Tohyama H, Katsura T, Kondo E, Kotani Y, Matsumoto H, et al. Effects of local administration of vascular endothelial growth factor on mechanical characteristics of the semitendinosus tendon graft after anterior cruciate ligament reconstruction in sheep. American Journal of Sports Medicine. 2006; 34(12): 1918-25. 5. Goradia VK, Rochat MC, Kida M and Grana WA. Natural history of a hamstring tendon autograft used for anterior cruciate ligament reconstruction in a sheep model. American Journal of Sports Medicine. 2000; 28(1): 40-6. 6. Goradia VK, Rochat MC, Grana WA, Rohrer MD and Prasad HS. Tendon-to-bone healing of a semitendinosus tendon autograft used for ACL reconstruction in a sheep model. American Journal of Knee Surgery. 2000; 13:143-51. 7. Weiler A, Hoffmann RFG, Bail HJ, Rehm O and Sudkamp NP. Tendon healing in a bone tunnel. Part II: histological analysis after biodegradable interference fit fixation in a model of anterior cruciate ligament reconstruction in sheep. Arthroscopy. 2002; 18(2): 124-35. 8. Soon MYH, Hassan A, Hui JHP, Goh JCH and Lee EH. An analysis of soft tissue allograft anterior cruciate ligament reconstruction in a rabbit model. A short-term study of the use of mesenchymal stem cells to enhance tendon osteointegration. American Journal of Sports Medicine. 2007; 35(6): 962-71. 9. Cheung KMC, Lu WW, Luk KDK, Wong CT, Chan D, Shen JX, et al. Vertebroplasty by use of a strontium-containing bioactive bone cement. Spine. 2005; 30(17S): S84-91. 10. Wong CT, Lu WW, Chan WK, Cheung KMC, Luk KDK, Lu DS, et al. In vivo cancellous bone remodeling on a strontium-containing hydroxyapatite (Sr-HA) bioactive cement. J Biomed Mater and Res. 2004; 68A:513-21. 11. Lu WW, Cheung KMC, Li YW, Luk KDK, Holmes AD, Zhu QA, et al. Bioactive bone cement as a principal fixture for spinal burst fracture: an in vitro biomechanical and morphologic study. Spine. 2001; 26(24):2684-91. 12. Wong CT, Chen QZ, Lu WW, Leong JCY, Chan WK, Cheung KMC, et al. Ultrastructural study of mineralization of a strontium-containing hydroxyapatite (Sr-HA) cement in vivo. J Biomed Mater and Res. 2004; 70:428-35. 13. Li YW, Leong JCY, Lu WW, Luk KDK, Cheung KMC, Chiu KY, et al. A novel injectable bioactive bone cement for spinal surgery: a developmental and preclinical study. J Biomed Mater and Res. 2000; 52:164-70.


Project Title:Development of a large animal knee osteoarthritis model using high tibial varus osteotomy in sheep
Investigator(s):Yau WP, Chiu PKY, Lie CWH
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:06/2009
Abstract:
Objective: The primary objective is to develop a large animal model of knee osteoarthritis (OA) with varus lower limb malalignment using high tibial varus osteotomy in sheep. The secondary objective is to evaluate the relationship between the different types of articular cartilage assessment between OA model induced by extra-articular (High tibial varus osteotomy) and intra-articular method (medial meniscectomy). Osteoarthritis of knee is the most common form of knee arthritis and a leading cause of total knee arthroplasty. It is estimated that there are around 140,000 total knee replacements performed in United States each year (1). As a leading joint replacement center in Hong Kong, we observe that there is a relatively higher incidence of total knee replacement in Hong Kong when compared with the Caucasian statistics. We also observed that there was a higher degree of coronal and sagittal plane bowing of both femur and tibia (2-3) and more severe varus malalignment in coronal plane in Chinese population receiving total knee arthroplasty (4). This observation was echoed by similar studies reported in other oriental population, including Japan (5). We had studied the osteometry of lower limb in fifty asymptomatic young skeletally mature volunteers. We found that there was a statistically significant more varus alignment of lower limb and varus inclination of the joint line in the coronal plane in Chinese when compared with similar Caucasian report (6). Varus malalignment of the lower limb is important in the development and progression of osteoarthritis of knee (7-8). During activities of daily living, the medial compartment is loaded about 50% more than the lateral compartment due to the adduction moment produced during weight bearing (9–11). Varus malalignment shifts the load bearing axis to the medial which further increases the total load passing the medial compartment during gait (12). A vicious cycle will continue and lead to further progression of medial compartment OA. The observation of a more varus lower limb alignment in asymptomatic young skeletally mature Chinese volunteers (6) leads us to believe that the increased incidence of end stage osteoarthritis of knee joint observed in Chinese population may be partially related to this mechanical disadvantage in the osteometry of lower limb (i.e. varus lower limb malalignment), which is present after skeletal maturity in Chinese. However, the available knee osteoarthritis models in animal fail to address the significance of this mechanical disadvantage in production of knee joint osteoarthritis. The current available knee osteoarthritis animal model include [1] spontaneous model in aging animals, [2] genetically modified mice, [3) surgically induced (including meniscectomy and Anterior cruciate ligament deficient knee model), [4] enzymatically induced and [5] chemically induced model (13-15). Spontaneous models best mimic the slow progression of the human disease but they are time consuming and progression is variable (16). Genetically modified mice constitute the best tools for understanding the functional role of specific molecules in cartilage homeostasis and OA pathology. However, the size of the animal makes this model not a good representative of human condition, especially in weight bearing areas including the knee and hip joint (17). Surgically (18) and enzymatically induced models (19) develop rapid and reproducible damage, but might be more relevant to the traumatic forms of OA than to the classical degenerative form of OA. Chemically induced models develop even more rapid damage, but their physiological relevance is debatable (20). All of the above osteoarthritis animal models fail to address the contribution of the adverse mechanical lower limb environment (i.e. varus malalignment of lower limb in coronal plane) in the pathophysiology of osteoarthritis. In view of the prevalence of varus malalignment in the young healthy local Chinese population, the development of such a malalignment osteoarthritis model in a large animal setting is essential for us to understand the development of osteoarthritis in Chinese and to evaluate different type of potential interventional treatment in our local population. Thus, we propose to develop a new large animal model of knee osteoarthritis using varus high tibial osteotomy in Merino sheep to reproduce the adverse mechanical malalignment that is observed in Chinese population. Reference: 1. Mahomed: Epidemiology of Total Knee Replacements in United States. Medicare Population. Journal Bone and Joint Surgery, 2005; 87A:1222-8. 2. Yau WP: Coronal Bowing of the Femur and Tibia in Chinese – Its incidence and effect in total knee arthroplasty planning. J Orthop Surg 2007; 15:32-6. 3. Tang WM: Sagittal bowing in distal femur and total knee arthroplasty. J Orthop Research 2005, 23:41-5. 4. WP Yau: Computer navigation did not improve alignment in a lower-volume total knee practice. Clinical Orthopaedics and Related Research 2008, 466(4):935-45. 5. Nagamine: Anatomical variations should be considered in total knee arthroplasty. J Orthop Science 2000, 5:232-7. 6. WM Tang: Axial alignment of the lower extremity in Chinese Adults. J Bone Joint Surg 2000, 82A, 1603-8. 7. Brouwer: Association between valgus and varus alignment and the development and progression of radiographic osteoarthritis of the knee. Arthritis Rheum 2007; 1204-11. 8. Sharma: The role of knee alignment in disease progression and functional decline in knee osteoarthritis. JAMA 2001; 286:188-95. 9. Andriachhi: Dynamics of knee malalignment. Orthop Chin North Am 1994; 25: 395-403. 10. Morrison: The mechanics of the knee joint in relation to normal walking. J Biomech 1970; 3: 51-56. 11. Moskowitz: Osteoarthritis 4th edition: Ch 21B: p.395-414. 12. Hsu RW: Normal axial alignment of the lower extremity and load-bearing distribution at the knee. Clin Orthop 1990; 255: 215-27. 13. Bendele: Animal models of osteoarthritis. J Musculoskelet Neuronal Interact 2001; 1: 363-376. 14. Van den Berg WB: Lessons from animal models of osteoarthritis. Curr Opin Rheumatol 2001; 13: 452-456. 15. Ameye: Animal models of osteoarthritis: lessons learned while seeking the “Holy Grail” Curr Opin Rheumatol 2006, 18:537-47. 16. Jallali: Vulnerability to ROS-induced cell death in ageing articular cartilage: analysis of cell density, apoptosis-related gene expression and mechanism controlling chondrocyte apoptosis. Osteoarthritis Cartilage 2004, 12:917-23. 17. Glasson: Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature 2005, 434:644-8. 18. Quasnichka: Cruciate ligament laxity and femoral intercondylar notch narrowing in early-stage knee osteoarthritis. Arthritis Rheum 2005, 52:3100-9. 19. Blom: Synovial lining macrophages mediate osteophyte formation during experimental osteoarthritis. Osteoarthritis Cartilage 2004, 12:627-35. 20. Bove: Weight bearing as a measure of disease progression nand efficacy of anti-inflammatory compounds in a model of monosodium iodoacetate-induced osteoarthritis. Osteoarthritis Cartilage 2003, 11:821-30.


Project Title:The Fourth International Congress of Chinese Orthopaedic Association Prospective Case Control Study comparing "Transportal"versus "Transtibial" technique in preparation of femoral tunnel in Arthroscopic Assisted Single bundle Hamstring Anterior Cruciate Ligament Reconstruction
Investigator(s):Yau WP
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:11/2009
Completion Date:11/2009
Abstract:
N/A


List of Research Outputs

Chan C.X. and Yau W.P., Mechanical Alignment of Lower Limbs in Chinese: Incidence and Association with Bowing in Various Segments of Femur and Tibia, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Chan C.X. and Yau W.P., Mechanical Alignment of Lower Limbs in Chinese: Incidence and Association with Bowing in Various Segments of Femur and Tibia, The Forth International Congress of Chinese Orthopaedic Association. 2009.
Chiu P.K.Y., Cheng H.C., Yau W.P., Tang W.M. and Ho H.S., Reading Radiographs after Total Knee Arthroplasty, Hong Kong Journal of Orthopaedic Surgery . 2010, 14 (1): 25 - 39.
Fok A.W.M. and Yau W.P., Isolated Bundle Tears of Anterior Cruciate Ligament: Incidence, Correlation with Tests and Injury to Surgery Duration, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Fok A.W.M. and Yau W.P., Isolated Bundle Tears of Anterior Cruciate Ligament: Incidence, Correlation with Tests and Injury to Surgery Duration, The Forth International Congress of Chinese Orthopaedic Association. 2009.
Kuang G., Yau W.P., Lu W.W. and Chiu P.K.Y., Methods in enhancing osteointegration of soft tissue grafts within the bone tunnels in anterior cruciate ligament reconstruction, The 29th Annual Congress Of The Hong Kong Orthopaedic Association, Hong Kong Journal Of Orthopaedic Surgery. 2009, 13: S125.
Kuang G.M., Wong C.W., Yeung K.W.K. and Yau W.P., Development of Small Animal Model for Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Kuang G.M., Yau W.P., Lu W.W. and Chiu P.K.Y., Methods in Enhancing Osteointegration of Soft Tissue Grafts within the Bone Tunnels in Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Kuang G.M., Yau W.P. and Lu W.W., Osteointegration of soft tissue grafts within the bone tunnels in Anterior Cruciate Ligament reconstruction can be enhanced, Knee Surgery, Sports Traumatology, Arthroscopy . 2009, E publication.
Wong C.W., Yeung K.W.K., Kuang G.M., Chu P.K. and Yau W.P., Mechanical and In-Vitro Analyses of Plasma Surface-modified Ti-6AI-4V Alloy Interference Screw for Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Yau W.P., ACL Reconstruction, Depuy Mitek Shoulder Cadaver Workshop. 2009.
Yau W.P., Case of the Month, SICOT e-Newsletter. 2009, 10.
Yau W.P., Chiu P.K.Y. and Yan C.H., Cutting errors in Total Knee Replacement, 10th Annual Meeting of Computer Assisted Orthopaedic Surgery – International. 2010.
Yau W.P., Ipsilateral Hamstring Autograft Single-bundle versus Double-bundle Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Yau W.P., Length and Size of Medial Hamstring Tendon Graft in Southern Chinese, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Yau W.P. and Chiu P.K.Y., Ligament balance for varus knee in computer navigation total knee replacement, 10th Annual Meeting of Computer Assisted Orthopaedic Surgery – International. 2010.
Yau W.P., Prospective Case Control Study comparing “Transportal” versus “Transtibial” technique in preparation of femoral tunnel in Arthroscopic Assisted Single bundle Hamstring Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Yau W.P., Prospective Case Control Study comparing “Transportal” versus “Transtibial” technique in preparation of femoral tunnel in Arthroscopic Assisted Single bundle Hamstring Anterior Cruciate Ligament Reconstruction, The Forth International Congress of Chinese Orthopaedic Association. 2009.
Yau W.P., Should adolescent ACL injury be treated surgically?, 7th Hong Kong International Orthopaedic Forum – Common Orthopaedic Myths. 2010.
Yau W.P., Sports and Arthroscopy, Preparatory Course for HKCOS / FRCS (Orth) Examination – 2010. 2010.
Yau W.P. and Fang C., Validation of International Knee Documentation Committee Subjective Knee Form (Chinese Translation – The University of Hong Kong Version), 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Yau W.P., Where are we with the double bundle ACL Reconstruction?, Hong Kong Orthopaedic Association – Sports Medicine Chapter Trimonthly Meeting. 2010.
Zhao Z.Y., Chiu P.K.Y., Zhang S.D., Ma W.H., Yau W.P., Tang W.M., Wang S.J. and Wang W.G., A study on the effects of tibial cutting jig fixation on varus-valgus and anteroposterior slope of the tibial osteotomy, 胫骨平台切朴的因定方法对胫骨平台截骨在内外翻和前后倾方面影响的研究, Chinese Journal of Joint Surgery . 中国关节外科杂志, 2009, 3: 750 - 754.


Researcher : Yee FYA

List of Research Outputs

Yee F.Y.A., Cheung K.M.C. and Chan D., Transcriptome and proteome of the intervertebral disc in health and disease, 2010.


Researcher : Yee PK

List of Research Outputs

Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in Southern Chinese: a descriptive and comparative study of 91 cases, 29th Annual Congress of the Hong Kong Orthopaedic Association. Hong Kong, SAR China, 2009.
Yee P.K., Samartzis D., Wong Y.W., Luk K.D.K. and Cheung K.M.C., Infective spondylitis in southern Chinese: a descriptive and comparative study of 91 cases, Spine. 2010, 35: 635-641.


Researcher : Yeung CY

List of Research Outputs

Yeung C.Y., Leung K.Y., Yeung K.W.K., Kao R.Y.T., Chu P.K., Cheung K.M.C. and Luk K.D.K., Inhibition of metallic implant-related bacterial infections by novel water plasma surface treatment, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. PCT Patent Application (No.: PCT/CN2010/000216) Filed: Feb 18, 2010., PCT Patent Application. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010., U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010.. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, PCT Patent Application (No.: PCT/CN2010/000216) filed 18 February 2010. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, U.S. Non-Provisional Patent (No.: 12/706,483). filed 16 February 2010. 2010.


Researcher : Yeung CY

List of Research Outputs

Yeung C.Y., Leung K.Y., Yeung K.W.K., Kao R.Y.T., Chu P.K., Cheung K.M.C. and Luk K.D.K., Inhibition of metallic implant-related bacterial infections by novel water plasma surface treatment, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. PCT Patent Application (No.: PCT/CN2010/000216) Filed: Feb 18, 2010., PCT Patent Application. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010., U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010.. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, PCT Patent Application (No.: PCT/CN2010/000216) filed 18 February 2010. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, U.S. Non-Provisional Patent (No.: 12/706,483). filed 16 February 2010. 2010.


Researcher : Yeung KWK

Project Title:Materials Science & Technology 2006 Conference and Exhibition New Plasma Surface Treated Memory Alloys: Towards A New Generation of "Smart" Orthopaedic Materials
Investigator(s):Yeung KWK
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:10/2006
Abstract:
N/A


Project Title:Development of Novel Biodegradable Metallic Materials for Orthopedics
Investigator(s):Yeung KWK, Chan D, Cheung KMC, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2008
Abstract:
1 To perform comprehensive mechanical, biomechanical, short term in-vitro and in-vivo biological studies in order to assess the feasibility of adopting plasma-treated biodegradable metals in orthopedic implants, and 2 To examine the mechanical integrity of the plasma modified magnesium alloys under in-vitro environment and hydrogen release under in-vivo testing, and 3 To examine for toxic effects due to ion release and bone formation of plasma modified magnesium alloys at the implant-bone junction, and 4 To provide sufficient experimental data so as to optimize the plasma treatment conditions to obtain controllable degradation rates and optimal mechanical and biological surface properties in future.


Project Title:Development of Novel Plasma Modified Metallic Materials for Anterior Cruciate Ligament Reconstruction
Investigator(s):Yeung KWK, Chan D, Cheung KMC, Yau WP
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2009
Abstract:
1 Due to a 25% budget cut, it is not feasible to carry out the comprehensive mechanical characterizations, long term biological and biomechanical testing. As an alternative, we will only carry out surface mechanical testing, and use established cultured cell lines such as SaOs-2 (osteoblasts) and HEK-293 (fibroblasts) to evaluate biological performance in vitro. This approach will be within the revised budget. If necessary, the long term tests will be evaluated in a future RGC or ITF application following the in vitro analysis. Thus, the revised objectives are: to modify the surface chemistry and nano topography of current metallic interference screw using PIII technology so as to enhance (a) osteointegration in the bone-screw interface; (b) osteointegration in the graft-screw interface for bone-patellar tendon-bone graft and (c) Sharpey fibre and firbrocartilage formation in graft-screw interface for soft tissue graft (e.g. hamstring tendons graft), and 2 to perform mechanical characterizations in order to assess surface mechanical properties of modified samples, and 3 to evaluate bioactivity of the surface modified metallic interference screw using short term in-vitro tests in order to screen out the sample with best biological performance for the subsequent in-vivo biological studies, and 4 to comprehensively study the short term in-vivo biological response such as tensile property of inserted graft, osteogenesis, Sharpey fibers and fibrocartilage formation in order to assess the feasibility of plasma modified metallic interference screws in future ACL reconstruction.


Project Title:22th European Conference of Biomaterials (ESB 2009) Oxygen plasma implanted titanium surface to resist Staphylococcus aureus
Investigator(s):Yeung KWK
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:09/2009
Completion Date:09/2009
Abstract:
N/A


Project Title:Improvement of the biofunctionality of PEEK materials using novel plasma surface technology
Investigator(s):Yeung KWK, Cheung KMC, Luk KDK, Pan H
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:01/2010
Abstract:
Objective: To investigate the feasibility of biofunctionality enhancement of orthopaedic polymeric material (PEEK) by using novel plasma surface technology. Seed Funding is sought to perform surface characterizations and in-vitro biological tests of those plasma treated materials. Metallic biomaterials such as titanium alloys in orthopaedic implantation are popular due to their excellent corrosion resistance, high mechanical strength as well as biocompatibility. However, due to the mismatched mechanical properties between metals and human bone, the high mechanical strength has been suspected to correlate to a serious post-operative complication called stress-shielding effect [1, 2]. This unavoidable complication will be therefore leading to bone loss and bone degradation around implant, thereby resulting to implant loosening and failure of implant. In addition, metallic materials are radio-opaque in essence. Hence, the blackout of surgical site during radiography assessment is another concern. Therefore, a new polymeric biomaterial polyetheretherketone (PEEK) has been developed in which this new biopolymer is able to remedy those major concerns. The mechanical properties of this polymer are close to that of human bones, thereby significantly reducing the post-operative stress shielding effect. In addition, its radiolucent properties greatly facilitate the x-ray assessment. Hence, it has attracted many applications in orthopaedics such as intervertebral spacer, spinal cage and prosthesis. Although clinical studies revealed that patients implanted with implants made of PEEK material can achieve bony fusion at long-term follow-up[3-6], practical problems such as implant migration and subsidence of PEEK implant have been observed. The reasons have not been well addressed and yet a concern on the tissue-implant integration using PEEK material is raised . PEEK is described as a bio-inert material in which the material neither stimulates nor suppresses the response of cells. Therefore, unsatisfactory bone-implant interaction is one of major suspects. Various techniques to turn on the bioactivity of PEEK material have been investigating by others and their efforts mainly focus on two aspects: (1) fabrication of new composite incorporating PEEK and other bioactive materials, and (2) surface modification to activate the material bioactivity. The first approach has been demonstrated by incorporating beta-tricalcium phosphate powder into PEEK substrate while manufacturing [7]. The subsequent experimental results reported the new composite was not only unable to improve the bioactivity, but the reduction of cell proliferation in in-vivo test was also found as compared with pure PEEK material. Another composite, hydroxyaptite (HA) and PEEK, was fabricated as a new bioactive material. In immersion testing with simulated body fluid, apatite layer formation was observed on the surface, whereas pure PEEK was not found [8]. However, the elastic modulus of original PEEK material has been altered while mixing with HA powders [9]. Incorporating new material into original PEEK is therefore not a good way to improve its bioactivity. An alternative is to establish biofunctional groups over material surface. To avoid altering the original mechanical properties of PEEK material, surface modification seems to be a light on this issue. A study suggested that tissue-material interaction is highly governed by surface topography, chemistry, roughness and micro-pattern [10]. Bone-implant interaction refers to the ability of cell deposition on implant surface. It is believed that cell adhesion on biomaterial starts with adhesion of membrane fusion proteins such as fibronectin (FN), vitronectin (VN) and laminin (LM) to the surface. Cells then interact with these adhered proteins in a receptor-ligand interaction through specific cell-surface receptors [11], thereby leading to cell attachment. If material surface favors to these interactions, it can be regarded as bioactive material in which direct formation of a bond between tissue and material is resulted [12]. Conventional surface ion implantations with oxygen, ammonia and argon to improve the integration of tissue-material interface of PEEK were studied long time ago [13-17]. However, coating will be delaminated while subjecting to bending moment. Among various surface treatments, plasma immersion ion implantation (PIII) is an attractive approach, as modified surface does not delaminate as other conventional coatings while subjecting to mechanical loading [14]. This surface technology can only modify surface chemistry of materials by bombarding and implanting plasma ions onto the surface, and without compromising the bulk mechanical properties. New biofunctional groups may be therefore established on material surface due to the molecules’ interaction. In addition to the improvement of corrosion resistance, plasma surface modification has been applied to the enhancement of blood compatibility, the control of protein adsorption and cell adhesion as well as tissue growth [18]. Few plasma treatments had been employed to modify polymer surface properties. For instance, PIII has been using to modify the bioactivity of polylactide material [19]. The results suggested that the growth and attachment of osteoblasts on the plasma-treated polylactide membrane are significant superior to other untreated controls [20]. Another study suggested that PEEK materials treated with ammonia/argon microwave plasma could stimulate proliferation of fibroblasts and osteoblasts [17]. In general, cell proliferation on such modified surface has been improved in-vitro due to the formation of carboxyl, nitrogen- and oxygen-containing functional groups on the material surface and carboxyl group has been identified as a significant matter in protein adhesion early in 1984 [14]. Although those previous studies successfully demonstrated promising in-vitro results, the reality of how the surface treated materials react with tissues under in-vivo environment is still not yet known. Therefore, we aim at adopting this advance surface technique to establish the biofunctional groups such as carboxyl, nitrogen-containing and oxygen-containing groups on PEEK surface so as to enhance its bioactivity in-vitro and tissue-material interaction in-vivo. If succeeded, we could establish a simple and economy method to enhance the biofunctionality of PEEK materials. The reason to apply for seed funding is to study which PIII treatments such as ammonium, oxygen and nitrogen may achieve better biofunctionality on PEEK materials. After PIII treatments simple cell cultures will be used to evaluate the biofunctionality of treated surfaces. Once the results are available, we will submit a more elaborate application to external funding bodies such as RGC for a comprehensive study of these plasma-treated PEEK materials in clinical use, and ITF for commercialization of the processes. We believe that the success of the external grant applications can be significantly increased if such preliminary work can be done in advance, and in particular, proof of Principal experiments are needed for such competitive grant applications. # Please refer to Appendix I for the list of references.


Project Title:Development of a novel method to suppress bacterial adhesion on orthopaedic implant surface using plasma-based technology
Investigator(s):Yeung KWK, Cheung KMC, Kao RYT, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:General Research Fund (GRF)
Start Date:01/2010
Abstract:
1) The revised objectives of this research grant are sought: to modify the surface chemistry and topography of currently used medical grade titanium materials using oxygen and water PIII; 2) to evaluate the bioactivity and the antibacterial properties of the surface modified materials using short term in-vitro tests in order to attain the best biological and antibacterial performance for the subsequent in-vivo biological studies; 3) to comprehensively study the short term in-vivo antibacterial and biological response of the plasma modified metallic materials under the in-vivo environment in order to pave the way for future human clinical trials.


Project Title:Prevention of orthopaedic implant related bacterial infections using novel plasma surface treatments
Investigator(s):Yeung KWK, Leung FKL, Kao RYT, Cheung KMC, Luk KDK, Tam V
Department:Orthopaedics and Traumatology
Source(s) of Funding:AO Foundation Research Fund
Start Date:02/2010
Abstract:
The plasma treated metallic surfaces can reduce bacterial adhesion to the implant surface, therefore reducing/preventing bacterial infection in-vivo when used for implantation. The aim of the project is to establish the correlations between the antibacterial capability of oxygen and water plasma engineered surfaces and bacterial adhesion by altering implantation parameters such as voltage, time and frequency, and to also compare the effectiveness of these plasma samples treated with various implantation parameters in terms of the suppression of S. aureus and P. aeruginosa adhesion and growth by conducting short term in-vitro and in-vivo biological studies. (1) To establish the correlations between the plasma treatment parameters and bacterial reduction; (2) To characterize the surface properties of the PIII-treated materials including topography, roughness, pattern, chemistry etc.; (3) To conduct both in-vitro and in vivo tests to validate this application for the use of orthopaedic implants (4) To optimize plasma treatment parameters in order to obtain better in-vitro and in-vivo results.


Project Title:Prevention of Orthopaedic Implant Related Bacterial Infections by Using Novel Plasma Surface Treatments
Investigator(s):Yeung KWK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Innovation and Technology Fund Internship Programme
Start Date:04/2010
Abstract:
Patients with orthopaedic fractures or deformities are treated by surgically implanting metallic materials, as metals are more appropriate for load-bearing applications. However, debridement of wound or implant removal due to bacterial infection is occasionally needed in such orthopaedic cases if conventional antibiotic therapy fails. To date, implants coated with antibiotics, nitric oxide, titanium oxide, silver and copper ions have been used to solve problems associated with bacterial implant infection. However, the complications related to low yield of antibiotics, expensive processing, unknown compatibility with surrounding tissues and expeditious degradation of coatings are still concerned. The approach suggested in this proposal is to alter the surface chemistry and topography of the materials using plasma immersion ion implantation technology in order to suppress the adhesion of bacteria on the material surfaces. Orthopaedic implants modified by this novel surface approach should be more durable and biocompatible, as this method does not depend on the release of coatings. The modified surface has the ability to resist bacterial adhesion intrinsically after plasma surface treatment. This study is vital to achieving superior inhibition of bacteria on the metallic implant surface clinically. The reason to apply this ITF funding is to characterize which implantation parameters such as implantation source, voltage, frequency and time can achieve superior antibacterial property on medical grade titanium alloys. When all the characterizations have been completed by the end of this grant, we plan to submit another ITF TCFS application and/or solicit supports from industrial partners to commercialize these plasma-treated implant materials. For instance, tuning-up of the plasma surface treatment process into industrial scale is suggested. We believe that the potential of commercialization will be significantly enhanced if the preliminary work described in this proposal can be carried out in advance. The objectives of this ITF Tier 3 funding include: (1) to modify the surface chemistry and topography of currently used medical grade titanium materials with the use of various plasma implantation parameters, (2) to evaluate the surface mechanical, biofunctionality and the antibacterial properties of the surface modified materials using short term in-vitro tests in order to attain the best biological and antibacterial performance for the subsequent in-vivo biological studies, and (3) to comprehensively study the short term in-vivo antibacterial and biological response of the plasma modified metallic materials under the in-vivo environment in order to pave the way for future human clinical trials and commercialization.


Project Title:Prevention of Orthopaedic Implant Related Bacterial Infections by Using Novel Plasma Surface Treatments
Investigator(s):Yeung KWK, Kao RYT, Cheung KMC, Luk KDK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Innovation and Technology Support Programme (Tier 3)
Start Date:04/2010
Abstract:
Patients with orthopaedic fractures or deformities are treated by surgically implanting metallic materials, as metals are more appropriate for load-bearing applications. However, debridement of wound or implant removal due to bacterial infection is occasionally needed in such orthopaedic cases if conventional antibiotic therapy fails. To date, implants coated with antibiotics, nitric oxide, titanium oxide, silver and copper ions have been used to solve problems associated with bacterial implant infection. However, the complications related to low yield of antibiotics, expensive processing, unknown compatibility with surrounding tissues and expeditious degradation of coatings are still concerned. The approach suggested in this proposal is to alter the surface chemistry and topography of the materials using plasma immersion ion implantation technology in order to suppress the adhesion of bacteria on the material surfaces. Orthopaedic implants modified by this novel surface approach should be more durable and biocompatible, as this method does not depend on the release of coatings. The modified surface has the ability to resist bacterial adhesion intrinsically after plasma surface treatment. This study is vital to achieving superior inhibition of bacteria on the metallic implant surface clinically. The reason to apply this ITF funding is to characterize which implantation parameters such as implantation source, voltage, frequency and time can achieve superior antibacterial property on medical grade titanium alloys. When all the characterizations have been completed by the end of this grant, we plan to submit another ITF TCFS application and/or solicit supports from industrial partners to commercialize these plasma-treated implant materials. For instance, tuning-up of the plasma surface treatment process into industrial scale is suggested. We believe that the potential of commercialization will be significantly enhanced if the preliminary work described in this proposal can be carried out in advance. The objectives of this ITF Tier 3 funding include: (1) to modify the surface chemistry and topography of currently used medical grade titanium materials with the use of various plasma implantation parameters, (2) to evaluate the surface mechanical, biofunctionality and the antibacterial properties of the surface modified materials using short term in-vitro tests in order to attain the best biological and antibacterial performance for the subsequent in-vivo biological studies, and (3) to comprehensively study the short term in-vivo antibacterial and biological response of the plasma modified metallic materials under the in-vivo environment in order to pave the way for future human clinical trials and commercialization.


Project Title:Prevention of Orthopaedic Implant Related Bacterial Infections by Using Novel Plasma Surface Treatments
Investigator(s):Yeung KWK
Department:Orthopaedics and Traumatology
Source(s) of Funding:Innovation and Technology Fund Internship Programme
Start Date:04/2010
Abstract:
Patients with orthopaedic fractures or deformities are treated by surgically implanting metallic materials, as metals are more appropriate for load-bearing applications. However, debridement of wound or implant removal due to bacterial infection is occasionally needed in such orthopaedic cases if conventional antibiotic therapy fails. To date, implants coated with antibiotics, nitric oxide, titanium oxide, silver and copper ions have been used to solve problems associated with bacterial implant infection. However, the complications related to low yield of antibiotics, expensive processing, unknown compatibility with surrounding tissues and expeditious degradation of coatings are still concerned. The approach suggested in this proposal is to alter the surface chemistry and topography of the materials using plasma immersion ion implantation technology in order to suppress the adhesion of bacteria on the material surfaces. Orthopaedic implants modified by this novel surface approach should be more durable and biocompatible, as this method does not depend on the release of coatings. The modified surface has the ability to resist bacterial adhesion intrinsically after plasma surface treatment. This study is vital to achieving superior inhibition of bacteria on the metallic implant surface clinically. The reason to apply this ITF funding is to characterize which implantation parameters such as implantation source, voltage, frequency and time can achieve superior antibacterial property on medical grade titanium alloys. When all the characterizations have been completed by the end of this grant, we plan to submit another ITF TCFS application and/or solicit supports from industrial partners to commercialize these plasma-treated implant materials. For instance, tuning-up of the plasma surface treatment process into industrial scale is suggested. We believe that the potential of commercialization will be significantly enhanced if the preliminary work described in this proposal can be carried out in advance. The objectives of this ITF Tier 3 funding include: (1) to modify the surface chemistry and topography of currently used medical grade titanium materials with the use of various plasma implantation parameters, (2) to evaluate the surface mechanical, biofunctionality and the antibacterial properties of the surface modified materials using short term in-vitro tests in order to attain the best biological and antibacterial performance for the subsequent in-vivo biological studies, and (3) to comprehensively study the short term in-vivo antibacterial and biological response of the plasma modified metallic materials under the in-vivo environment in order to pave the way for future human clinical trials and commercialization.


List of Research Outputs

Cheung K.M.C., Yeung K.W.K., Lu W.W. and Chung C.Y., Device for correcting spinal deformities, Japan patent no. 4423197, granted on December 11, 2009, 2009.
Cheung K.M.C., Yeung K.W.K., Lu W.W., Poon T.L. and Chow S.P., Fixation device, US Regular patent no. 7,695,471, granted on April 13, 2010, 2010.
Chu C.L., Hu T., Yin L.H., Pu Y.P., Dong Y.S., Lin P.H., Chung C.Y., Yeung K.W.K. and Chu P.K., Microstructural Characteristics and Biocompatibility of a Type-B Carbonated Hydroxyapatite Coating Deposited on NiTi Shape Memory Alloy. , Bio-Medical Materials and Engineering. 2009, 19(6): 401-408.
Chu C.L., Guo X.X., Sheng X.B., Dong Y.S., Lin P.H., Yeung K.W.K. and Chu P.K., Microstructure, Nickel Suppression, and Mechanical Characteristics of Electropolished and Photoelectrocatalytically Oxidized Biomedical Nickel Titanium Shape Memory Alloy, Acta Biomaterialia. 2009, 5(6): 2238-2245.
Hu T., Chu C.L., Xin Y.C., Wu S.L., Yeung K.W.K. and Chu P.K., Corrosion Products and Mechanism on NiTi Shape Memory Alloy in Physiological Environment. , Journal of Materials Research. 2010, 25(2): 350-358.
Kuang G.M., Wong C.W., Yeung K.W.K. and Yau W.P., Development of Small Animal Model for Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Kwok D.T.K., Wang H.Y., Zhang Y.M., Yeung K.W.K. and Chu P.K., Effects of Long Pulse Width and High Pulsing Frequency on Surface Super-Hydrophobicity of Polytetrafluoroethylene in Quasi Direct-Current Plasma Immersion Ion Implantation., Journal of Applied Physics. 2009, 105(5): 053302-1 - 053302-4.
Lu W.W., Tang W.K., Yeung K.W.K. and Luk K.D.K., In vitro kinematics study of human lumbar facet joints, Combined SICOT/RCOST 2009 Annual Meeting, Pattaya, Thailand, October 29-November 1, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of PEEK Bioactivity by Plasma Surface Modification., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug-3Sep 2009. Poster presentation.. 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of Osteoblast Attachment and Growth on PEEK by Water and Ammonia Plasma Surface Treatment. (6-9 Mar 2010), 56th Annual Meeting of the Orthopaedic Research Society, New Orleans. 2010, Paper 1193.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., Improvement of osteoblast attachment and growth on PEEK by water and ammonia plasma surface treatment, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Lui S.C., Yeung K.W.K., Chu P.K., Kwok T.K., Pan H., Luk K.D.K. and Cheung K.M.C., Plasma-modified polyetheretherketone for orthopaedic applications: bulk and surface mechanical characterisations, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Lui S.C., Cheung K.M.C. and Yeung K.W.K., Surface bioactivity enhancement of polyetheretherketone by plasma immersion ion implantation, 2009.
Lui S.C., Yeung K.W.K., Chu P.K., Luk K.D.K. and Cheung K.M.C., The Effect of Plasma Technology in Bioactivity Enhancement of PEEK. , 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep2009. Poster presentation.. 2009.
Tang W.K., Luk K.D.K., Yeung K.W.K. and Lu W.W., In vivo kinematics of the lumbar facet joints for the development of a facet fixator, 2009.
Tang W.K., Yeung K.W.K., Lu W.W. and Luk K.D.K., In-vitro kinematics study of human lumbar facet joints, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Tong W.Y., Yip K., Mo S., Cheung K.M.C., Luk K.D.K. and Yeung K.W.K., Promotion of bone formation at implant interface by local injection of mouse bone marrow-derived mesenchymal stem cells, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Tong W.Y.T., Liang Y.M., Tam V., Yip H.K., Kao R.Y.T., Cheung K.M.C., Yeung K.W.K. and Lam Y.W., Biochemical characterization of the cell-biomaterial interface by quantitative proteomics, Mol Cell Proteomics. 2010.
Wong C.W., Yeung K.W.K., Kuang G.M., Chu P.K. and Yau W.P., Mechanical and In-Vitro Analyses of Plasma Surface-modified Ti-6AI-4V Alloy Interference Screw for Anterior Cruciate Ligament Reconstruction, 29th Annual Congress of the Hong Kong Orthopaedic Association. 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A Biodegradable Polymer-Based Coating To Control The Performance Of Magnesium Alloy Orthopaedic Implants, Biomaterials. 2010, 31(8): 2084-209.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., A New PCL Based Porous Membrane to Control the Degradation of Biodegradable Metallic Materials., 2nd World Congress of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), Seoul, Korea, 31Aug–3Sep 2009. Poster presentation.. 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Tam V., Chu P.K., Luk K.D.K. and Cheung K.M.C., A biodegradable polymer-based coating to control the performance of magnesium alloy orthopaedic implants biomaterials, Biomaterials. 2010, 31: 2084-2096.
Wong H.M., Yeung K.W.K., Leung Y.L., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., In-vitro analysis of gene expressions of osteoblastic cells while exposing to various concentrations of magnesium ions, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Wong H.M., Yeung K.W.K., Lam K.O., Chu P.K., Luk K.D.K. and Cheung K.M.C., Reduction of the Corrosion Rate of Biodegradable Metallic Materials by Al2O3 PIII surface Treatment., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24pril2010.. 2010, 868.
Yeung C.Y., Leung K.Y., Yeung K.W.K., Kao R.Y.T., Chu P.K., Cheung K.M.C. and Luk K.D.K., Inhibition of metallic implant-related bacterial infections by novel water plasma surface treatment, 29th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 28-29, 2009.
Yeung K.W.K., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Tu H., Chu P.K. and Liu X.Y., Alterations of Surface Mechanics of Nickel Titanium Shape Memory Alloys after Plasma Surface Treatment. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010).. 2010, 110-111.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. PCT Patent Application (No.: PCT/CN2010/000216) Filed: Feb 18, 2010., PCT Patent Application. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication. U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010., U.S. Non-Provisional Patent (No.: 12/706,483). Filed: Feb 16, 2010.. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, PCT Patent Application (No.: PCT/CN2010/000216) filed 18 February 2010. 2010.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Yeung C.Y., Kao R.Y.T. and Chu P.K., Antibacterial surface and method of fabrication, U.S. Non-Provisional Patent (No.: 12/706,483). filed 16 February 2010. 2010.
Yeung K.W.K., Biological response of cells on plasma treated metallic surfaces, First Biomedical Materials Surface Engineering Young Academics Forum, Chengdu, PR China. 2009.
Yeung K.W.K., Biological response of cells on plasma treated metallic surfaces, Hubei University Biomaterial Seminar, Hubei University, Hubei, PR China. 2009.
Yeung K.W.K., Biological response of cells on plasma treated metallic surfaces, UTA/UTSW Bioengineering Videoconference Seminar, The University of Texas at Arlington, Texas, USA. 2009.
Yeung K.W.K., Lai D.W.T., Lai D.W.T., Lai D.W.T., Lu W.W., Luk K.D.K., Cheung K.M.C., Wu S.L., Wu S.L., Tu H., Chu P.K. and Chung C.Y., Biomechanical Analysis of Plasma Treated NiTi Materials for Spinal Deformity Correction., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May 2010). 2010, 24-25.
Yeung K.W.K., Certificate of Merit, Young Engineer Award, Hong Kong Institution of Engineers. 2010.
Yeung K.W.K., Editorial Board Member, Smart Materials Research Journal . Hindawi Publishing Corporation, 2010.
Yeung K.W.K., Lui S.C., Chu P.K., Luk K.D.K. and Cheung K.M.C., Enhancement of Cell Adhesion and Proliferation on Polyetheretherketone by Using Plasma Surface Modification., 2010 Annual Meeting & Exposition of the Society For Biomaterials, Seattle, Washington, 21-24April2010.. 2010, 864.
Yeung K.W.K., Lam K.O., Luk K.D.K., Cheung K.M.C., Wu S.L., Hu T., Liu X.Y., Chu C.L., Chu P.K. and Chan D., Long Term Cytocompatibility and In vivo Investigation of Nitrogen Plasma Implanted Shape Memory Alloy. , International Conference on Shape Memory ands Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 104-105.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K. and Chung C.Y., Metallic Implants for Spinal Deformity Correction, Advances in Biomedical Sciences and Engineering. USA, Bentham Science Publisher Ltd, 2009, 1-44.
Yeung K.W.K., Lam W.N., Natarajan D., Wu S.L., Hu T., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Nickel Ion Level in Scoliotic Patients Implanted with Nitrogen Plasma Surface Modified Nickel-Titanium Superelastic Spinal Implant., 3rd IEEE International NanoElectronics Conference (INEC), Hong Kong, 3-8 January 2010. Oral presentation.. 2010, Paper BC410, p. 134.
Yeung K.W.K., Kao R.Y.T., Chu P., Luk K.D.K. and Cheung K.M.C., Oxygen Plasma Implanted Titanium Surface to Resist Staphylococcus Aureus Adhesion., 22nd European Conference on Biomaterials (ESB2009), Lausanne, Switzerland, 7-11Sep 2009. Oral presentation.. 2009.
Yeung K.W.K., Cheung K.M.C., Luk K.D.K., Wu S.L., Liu X.Y., Chu C.L., Chung C.Y. and Chu P., Plasma Surface Modification of Nickel Titanium Shape Memory Alloys, Advances in Biomedical Sciences and Engineering. USA, Bentham Science Publisher Limited, 2009, 45-68.
Yeung K.W.K., Lam W.N., Wu S.L., Tu H., Chu P.K., Chung C.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Post-Operative Monitoring of Nickel Ion Level in Scoliotic Patients Operated with Novel Nickel-Titanium Superelastic Spinal Implant. , International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20 May 2010). 2010, 22-23.
Yeung K.W.K., Wu S.L., Tu H., Chu P.K., Liu X.Y., Lu W.W., Luk K.D.K. and Cheung K.M.C., Suppression of Surface Nickel Concentration of Nickel Titanium Shape Memory Alloys by Plasma Surface Treatment., International Conference on Shape Memory and Superelastic Technologies (SMST), Pacific Grove, California (16-20May2010). 2010, 112-113.


Researcher : Yip DKH

Project Title:To evaluate biomechanically the strongest host bone and allograft bone docking site geometry - for application in bone transplant patients undergoing limb salvage surgery
Investigator(s):Yip DKH, Chien EP
Department:Orthopaedics and Traumatology
Source(s) of Funding:Low Budget High Impact Programme
Start Date:11/2000
Abstract:
To find the strongest, bone docking site geometry between the host bone and massive bone allograft in limb salvage patients.


Project Title:Biomechanical evaluation of the effect of incomplete cortical erosion of a diaphyseal long bone
Investigator(s):Yip DKH
Department:Orthopaedics and Traumatology
Source(s) of Funding:Small Project Funding
Start Date:11/2002
Abstract:
To determine the critical failure strength of a diaphyseal long bone which has been eroded by metastatic disease?


Project Title:Strength reduction of suture due to abrasion of the interference screw
Investigator(s):Yip DKH
Department:Orthopaedics and Traumatology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To compare the failure strength of two types of sutures before and after the abrasive wear by the screw against bone.




Researcher : Yuen WYG

List of Research Outputs

Chan C.F., Yuen W.Y.G. and Leung F.K.L., Fixation of distal humeral fracture in elderly patient by locking compression plate Chinese Journal of Reparative and Reconstructive Surgery 2009:23(11):1285-89, 2009.
Leung F.K.L. and Yuen W.Y.G., Cadaveric study of Asian distal radius morphoanatomy in the application of volar locking plate fixation The 4th Chinese Orthopaedic Association Meeting, Xiamen, China, 19-22 November, 2009 , 2009.
Yuen W.Y.G. and Leung F.K.L., A Cadaveric study of Asian distal radius morphoanatomy in the application of volar locking plate fixation, 2009.
Yuen W.Y.G., Lau T.W. and Leung F.K.L., A Cadaveric study of Asian distal radius morphoanatomy in the application of volar locking plate fixation Poster presentation at the Orthopedic Research Society Meeting, New Orleans, USA, 6-9 March, 2010, USA, 2010.
Yuen W.Y.G. and Leung F.K.L., Best basic science paper: A cadaveric study of anatomical landmarks and guidelines for volar locking plate application in the distal radius , The Hong Kong Orthopaedic Association Annual Congress 2009. 2009.


Researcher : Zhang Z

Project Title:A Study of Robust Multivariate Local Polynomial Modelling and its Application for Estimating Time-varying Cortical Connectivity
Investigator(s):Zhang Z, Hung YS, Chan SC, Hu Y
Department:Electrical & Electronic Engg
Source(s) of Funding:Small Project Funding
Start Date:01/2010
Abstract:
The objective of this project is to develop a robust multivariate local polynomial modelling method for estimation of time-varying cortical functional connectivity from electroencephalography (EEG) data. The investigation of cortical functional connectivity (i.e., the correlation between spatially remote neurophysiological events) is a central research topic in neuroscience field, as it can convey important information for understanding the highly organized mechanisms of human brain during motor and cognitive tasks [1]. Nowadays, a number of approaches have been proposed for estimating the functional connectivity based on high-density EEG, and one type of effective approaches were developed on the basis of a multivariate autoregressive (MVAR) modelling of EEG [2, 3]. The MVAR coefficient estimates can provide a series of measures for the cortical connectivity in frequency domain, including the directed transfer function (DTF), the partial directed coherence (PDC), etc. The classical MVAR estimators cannot yield time-varying coefficient estimates and time-varying cortical connectivity patterns because they require the assumption of signal stationarity [4]. To address the problem, it is common to employ a sliding window to obtain the time-varying coefficient estimates locally. However, improper window length selection cannot adapt well to instantaneous EEG characteristics and may lead to a wrong interpretation of brain connectivity. Another problem of classical MVAR estimators is that these least-squares (LS) based estimators are very sensitive to short-duration and high amplitude impulsive artifacts, which are frequently encountered in EEG [5]. EEG trials contaminated by impulsive artifacts will generally be discarded from further analysis, which lowers the efficiency of the MVAR estimators. Therefore, there are two fundamental problems in existing MVAR estimators: 1) the difficulty to select a proper window length for time-varying estimates, and 2) the adverse effects of impulsive noise. These problems seriously impede the wider use of MVAR estimators, and limit our knowledge of detailed instantaneous cortical function connectivity. Recently, the investigators have proposed a local polynomial modelling (LPM) method for estimating a univariate time-varying linear regression model. LPM is a flexible and efficient technique in statistics and it has been widely applied in many research areas such as data smoothing, density estimation, and nonlinear modelling [6]. The proposed LPM-based linear regression estimator describes the time-varying regression coefficients locally by polynomials with a certain window, and solves the coefficients using weighted least-squares criterion. The investigators also developed a data-driven variable window selection scheme for LPM so that the LPM method had an outstanding adaptability to signal’s local characteristics. The attractive merits and encouraging performances of the LPM method prompt us to further investigate its extension to MVAR models, which can be viewed as a multivariate generalization of the univariate linear regression model. Furthermore, motivated by the sensitivity of LS-based MVAR estimators to impulsive noise, we shall employ M-estimation [7], instead of the LS estimation, in the LPM method to improve its robustness. Finally, the new M-estimation-based multivariate LPM method (MM-LPM) will be applied to estimate time-varying cortical functional connectively from real EEG data recorded during motor or cognitive tasks. The key issues of this project include: 1) to develop a robust multivariate local polynomial modelling method for estimation of MVAR models, and 2) to apply the method to fast and accurate estimation of time-varying cortical connectivity patterns from high-density EEG. 1) To develop a new robust multivariate LPM method with adaptive window selection and M-estimation. We shall develop an M-estimation-based multivariate LPM (MM-LPM) for estimating multivariate linear regression models and MVAR models on the basis of our previously proposed LS-based univariate LPM. Asymptotical properties of the MM-LPM estimator will be derived to reveal the relationships between bias/variance and window length. Then, we shall design a data-driven variable window selection scheme for the MM-LPM method. We expect that, compared with existing methods, the MM-LPM method can significantly improve the adaptation capability and robustness. A detailed comparison will be performed in a large variety of simulation conditions. 2) To estimate cortical connectivity from EEG by means of the new LPM estimator. We shall first test the MM-LPM-based MVAR estimator on simulated EEG data generated through known models, in order to provide quantitative measures about its reliability and applicability. The most efficient algorithms and parameters will be selected based on the comparison between the results and the imposed EEG model. A carefully-designed experiment paradigm will be used to assess the method in practice. The time-varying patterns, such as DTF and PDC, obtained by the new MVAR estimator are expected to reflect instantaneous EEG characteristics accurately and to exhibit stability in heavy noise environment. We shall further check whether our findings are consistent with previous evidences of brain connectivity. References: [1] K. J. Friston, “Models of brain function in neuroimaging,” Annu. Rev. Psychol., vol. 56, pp. 57-87, 2005. [2] A. Schlögl and G Supp, “Analyzing event-related EEG data with multivariate autoregressive parameters,” in Progress in Brain Research, vol. 159, C. Neuper and W. Klimesch, Ed. Oxford: Elsevier, 2006, pp. 135-147. [3] L. Astolfi, F. Cincotti, D. Mattia, M. G. Marciani, L. A. Baccala, F. de Vico Fallani, S. Salinari, M. Ursino, M. Zavaglia, L. Ding, J. C. Edgar, G. A. Miller, B. He, and F. Babiloni, “Comparison of different cortical connectivity estimators for high-resolution EEG recordings,” Hum. Brain Mapp., vol. 28, no. 2, pp. 143-157, Feb. 2007. [4] M. Ding, S. L. Bressler, W. Yang, and H. Liang, “Short-window spectral analysis of cortical event-related potentials by adaptive multivariate autoregressive modeling: data preprocessing, model validation, and variability assessment,” Biol. Cybern., vol. 83, no. 1, pp. 35-45, Jul. 2000. [5] L. C. Parra, C. D. Spence, A. D. Gerson, and P. Sajda, “Recipes for the linear analysis of EEG,” Neuroimage, vol. 28, no. 2, pp. 326-341, Nov. 2005. [6] J. Fan and I. Gijbels, Local Polynomial Modelling and Its Applications, London: Chapman and Hall, 1996. [7] P. J. Huber, Robust Statistics, NY: John Wiley, 1981.


List of Research Outputs

Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: Methods and comparisons. , J Electromyogr Kinesiol.. 2010, 20: 89-101.
Zhang Z., Liu H., Chan S.C., Luk K.D.K. and Hu Y., Time-dependent power spectral density estimation of surface electromyography during isometric muscle contraction: methods and comparisons, Journal of Electromyography and Kinesiology. 2010, 20: 89-101.


Researcher : Zhao X

List of Research Outputs

Pan H., Lam W.M.R., Zhao X., Li Z., Lu W.W., Wang D., Huang W., Lin K. and Chang J., Borosilicate for tissue engineering, World Association for Chinese Biomedical Engineers (WACBE). 2009.
Zhao X., Li Z., Liu W.G., Pan H., Lam R.W.M., Luk K.D.K. and Lu W.W., Characterization and evaluation of octa-arginine conjugated chitosan as non-biral vector for gene transfer, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Zhao X

List of Research Outputs

Pan H., Lam W.M.R., Zhao X., Li Z., Lu W.W., Wang D., Huang W., Lin K. and Chang J., Borosilicate for tissue engineering, World Association for Chinese Biomedical Engineers (WACBE). 2009.
Zhao X., Li Z., Liu W.G., Pan H., Lam R.W.M., Luk K.D.K. and Lu W.W., Characterization and evaluation of octa-arginine conjugated chitosan as non-biral vector for gene transfer, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


Researcher : Zhou G

Project Title:A mesenchymal stem cell and firefly luciferase-based high-throughput screening (HTS) platform for osteogenesis-promoting small molecule compounds
Investigator(s):Zhou G, Cheung KMC, Luk KDK, Kao RYT
Department:Orthopaedics and Traumatology
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:08/2008
Abstract:
Bone is a dynamic tissue undergoing continuing remodeling, in which mesenchymal stem cells (MSCs) play a key role in the balance of the bone remodeling cycle by synthesizing and depositing new bone matrix (osteogenesis). Reduced osteogenesis is associated with many skeletal disorders and there currently is no effective cure. On the other hand, pharmacologic targeting of stem cells in vivo toward particular lines of differentiation is an important aspect of regenerative medical research. Chemical genetics is an emerging technique that uses small chemical compounds to alter the functions of proteins to which they bind and subsequently the particular cellular processes. Potent and specific small-molecule modulators of stem cell differentiation may therefore be identified as a compound with great therapeutic values for degeneration diseases or injury. In the meantime, such chemicals may also serve as useful tools to study the molecular mechanisms of stem cell differentiation. In this respect, our ongoing research projects have identified several small molecule compounds that have potentials to promote chondrocytic function in vitro. In doing this research, we have the central facilities already established, including the chemical library containing a total of 50,240 small-molecule compounds, the liquid handling machine for 384-well plates and absorbance/luminancence detection system for HTS. Furthermore, a small molecule chemical, AOG001, provided by a collaborator, is under investigation in our laboratory for its in vivo effects in stimulating fracture union in an animal model. Normal osteogenesis is regulated by a variety of systemic and local factors that exert osteogenic functions on MSCs by activating the central transcription factor Runx2 through multiple interactive signaling pathways. Runx2 in turn regulates a cascade of osteogenic differentiation process, with the activation of a set of osteogenic genes and enzymes, including osteoterix, osteocalcin, alkaline phosphate (ALP), and type I collagen (Col1a). These molecules are often used as osteogenic markers to monitor the osteogenic-differentiating potential of a given cell type or the efficacy of an agent in promoting osteogenesis. However, most current methodologies for detecting the expression or activity of these osteogenic markers, such as real-time PCR, immunostaining and ALP activity measurement, are multiple-stepping, insensitive, or expensive, and in practice NOT suitable for high-throughput screening (HTS) of a library containing a large number of compounds. Firefly luciferase is widely used as a reporter for studying gene expression and regulation. It is a very sensitive genetic reporter due to the lack of any endogenous activity in mammalian cells. The Firefly luciferase is a 62 kD protein, which is active as a monomer. The enzyme catalyzes ATP-dependent D-luciferin oxidation by oxygen into oxyluciferin with emission of light centered on 560 nm. Using modified reagents and substrates that are commercially available, the enzymatic reaction yield a long lasting steady (3-5 hours) and highly sensitive (up to hundred thousand folds beyond the background noise) signal, which makes it very rather easy to quantify the firefly luciferase expression in mammalian cells. As a result, luciferase activity-based HTS approaches have proven successful in a number of pharmaceutical screening studies. Our recent work has been employing a mammalian expression plasmid vector PG70, where the luciferase expression is under the control of the Col1a promoter/enhancer that is always activated when Runx2 and other osteogenic-transcription factors are present in the same host cells. When the vector was transfected to the available murine MSC (e.g. CH3T10T1/2) or immortalized human MSC (e.g HtertMSC) lines undergoing osteogenic differentiation, we could see the luciferase activity dramatically increased within approximately 3-5 days. The transient transfection of the vector into these cells or primary MSCs, however, is not straightforwardly achievable and transfection efficiency may significantly vary time by time. The more efficient and stable techniques, such as a lentiviral system, which has also used in our other stem cell research projects, would be highly desirable. In this proposal, the ultimate goal is to establish a HTS platform for seeking small molecule chemicals that promote osteogenesis and are of potential pharmaceutical value. The following objectives are set: 1) To establish murine and human MSC lines infected with the recombinant lentivirus and stably expressing luciferase under the control of murine Col1a promoter/enhancer and the neomycine resistant phenotype; 2) To establish and validate the protocol monitoring the osteogenic differentiation of above cell lines using known osteogenesis-promoting agents by measuring the luciferase activity in comparison with the endogenous osteogenic markers; 3) To conduct the preliminary HTS experiments (in intermediate scale) for novel MSC osteogenesis-promoting small molecules.


Project Title:Xth European Cell & Materials (ECM) Conference: Stem Cells for Musculoskeletal Regeneration Isolation and characterization of cell subpopulation with stem Cell properties in human and monkey intervertebral disc (IVD)
Investigator(s):Zhou G
Department:Orthopaedics and Traumatology
Source(s) of Funding:URC/CRCG - Conference Grants for Teaching Staff
Start Date:06/2009
Completion Date:07/2009
Abstract:
N/A


List of Research Outputs

Liao H. and Zhou G., Development and progress of engineering of skeletal muscle tissue, In: Development and progress of engineering of skeletal muscle tissue. Liao H, Zhou GQ. Tissue Eng Part B Rev. 2009 Sep;15(3):319-31., Tissue Engineering. USA, Mary Ann Liebert Inc., 2009, 15: 319-31.
Liao H., Huang S., Cheung K.M.C., Chan D., Luk K.D.K. and Zhou G., Functional characterization of miRNA125b, miRNA140 and miRNA199a in chondrogenetic differentiation of mesenchymal stem cells, Cell Adhesion & Communication.. 2009, In press.
Luk K.D.K., Lu W.W., Zhou G., Peng S.L., Wang T. and Li Z., New Investigator Recognition Award., 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010. 2010.
Peng S.L., Liu X.W., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., Effect of different timing of strontium treatment on bone remodeling in ovariectomized rats, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.
Peng S.L., Liu X.W., Wang T., Zhou G., Li Z., Luk K.D.K., Guo X.X. and Lu W.W., In vivo anabolic effect of strontium on trabecular bone was associated with increased osteoblastogenesis of bone marrow stromal cells, 56th Annual Meeting of the Orthopaedic Research Society, New Orleans, Louisiana, March 6-9, 2010.


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