DENTAL FACULTY
| Researcher : Ajonuma LC |
| List of Research Outputs |
| Ajonuma L.C., Lu Q., Cheung B.P.K., Leung W.K., Samaranayake L.P. and Jin L.J., Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva, Cell Biology International. 2010, 34: 147-152. |
| Researcher : Anthonappa RP |
| List of Research Outputs |
| Anthonappa R.P. and King N.M., An unusual white lesion in a 10-month-old child, Dental Update. 2010, 37: 110-114. |
| Anthonappa R.P. and King N.M., An unusual white lesion in a 10-month-old child, Dental Update. 2010, 37: 110-114. |
| Omer R.S.M., Anthonappa R.P. and King N.M., Determination of the optimum time for surgical removal of unerupted anterior supernumerary teeth, In: Omer RSM, Anthonappa RP, King NM, Pediatric Dentistry. 2010, 32(1): 14-20. |
| Researcher : Au AHO |
| List of Research Outputs |
| Au A.H.O., Hui K.K.K., Lau E.W.S., Tong L.S.M. and Yiu C.K.Y., Guidelines on sedation in pediatric patients, Hong Kong Society of Pediatric Dentistry . 2009. |
| Researcher : Au-Yeung NKH |
| List of Research Outputs |
| Au-Yeung N.K.H. and Pow E.H.N., Minimally invasive sinus-lift technique with simultaneous installation of a wide diameter implant: a case report, Hong Kong Dental Journal. 2009, 6: 98-102. |
| Researcher : Bandara HMHN |
| List of Research Outputs |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development, BMC Microbiology. 2010, 10: 125 (pages 1-9). |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation, Journal of Medical Microbiology. 2009, 58: 1623-1631. |
| Researcher : Bendeus SAM |
| Project Title: | 80th Congress of the European Orthodontic Society Does Class II Correction Result Growth Modification? LeFort 1: Distraction Osteogenesis VS Conventional Surgery |
| Investigator(s): | Bendeus SAM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2004 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Bootvong K., Liu Z., McGrath C.P.J., Hagg E.U.O., Wong R.W.K., Bendeus S.A.M. and Yeung S., Virtual model analysis as an alternative approach to plaster model analysis: reliability and validity, The European Journal of Orthodontics. 2010, 32: 589-595. |
| Chua H.D.P., Bendeus S.A.M. and Cheung L.K., Cleft maxillary distraction versus orthognathic surgery – which one is more stable in 5 years?, Oral Surg, Oral Med, Oral Pathol, Oral Rad and Endod . 2010, 109: 803-14. |
| Researcher : Botelho MG |
| Project Title: | The effectiveness of professional and home bleaching in the treatment of tetracycline stained teeth |
| Investigator(s): | Botelho MG, Chan AWK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Low Budget High Impact Programme |
| Start Date: | 11/2000 |
| Abstract: |
| To compare the clinical efficacy or professional chairside bleaching with self-administered home bleaching for the aesthetic management of tetracycline stained teeth; to determine the cost effectiveness of the two bleaching procedures. |
| Project Title: | Antimicrobial effect of antibacterial conditioners on bacterial biofilms associated with dental caries |
| Investigator(s): | Botelho MG |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To determine the inhibitory effects of dental antibacterials and novel dental antibacterial tooth conditioners against bacteria in biofilms. |
| Project Title: | Reduction of bacterial adhesion using a novel flurocarbon coating |
| Investigator(s): | Botelho MG |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2006 |
| Abstract: |
| The objectives of the proposed study it to test a novel fluorocarbon coating applied using a novel application technique developed at the Hong Kong University of Science and Technology. In preliminary studies this nano-sized coating shows a reduced ability of single species of oral bacteria to adhere to the test surface. The aim of the study is to quantify this effect using dental pathogenic bacteria using a flow cell. It is aimed to test this coating on dental material used in prosthetic appliances. These surfaces in the oral environment have been shown to readily form dental biofilm growth that may contribute to dental caries or periodontal disease. The aim of the study is to analyze to what extent this nanolayer coating may have on preventing early biofilm formation. |
| Project Title: | The effect of Silver Diammine Fluoride on demineralized dentine in vitro |
| Investigator(s): | Botelho MG |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 10/2006 |
| Abstract: |
| The aims of this project are: 1 To investigate the effects of SDF on artificially de-mineralized dentin in vitro so as to determine its anticaries effect on treating active caries. This will involve an analysis of the effects of concentration and time of SDF on arresting caries as well as the variable of depth of the lesion. This will be investigated using micro-hardness testing, energy dispersive X-ray microanalysis (EDAX) and electron microprobe analysis (EPMA) 2 To investigate the potential effects of other caries arresting agents similar to SDF (eg Ag NO3, AgF, TiF6, SiF6, ZrF6, SnF6, GeF6, Sr F2, NaF, KI…) but that do not have the deleterious effects of blackening associated with Ag ions. 3 To investigate the effect of SDF on treated deminerlized dentine that is subjected to demineralization cycles and determine its resistance to acid attack. While various forms of fluoride have been shown to be effective in preventing dental caries, a relatively new form, Silver Diammine Fluoride (SDF) has shown potential in arresting existing enamel and dentine caries. The advantage of this agent is that it is clinically easy to apply, can be administrated by oral health care personal with minimal training and is cost effective. However, its main disadvantage is that the silver oxidizes and turns the tooth black. This would make it unacceptable by most adult patients in the general population if aesthetically visible |
| List of Research Outputs |
| Botelho M.G., "Introductory PBL Training Workshop Programme" , Penang International Dental College, Kuala Lumpur. Kuala Lumpur, Penang International Dental College, 2010, . |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience - Journal Based Learning and Fundamentals of Dental Research – a pilot programme, the 6th International Conference on PBL in Dentistry. Hong Kong, 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience-journal based learning and fundamentals of dental research-a pilot programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.26. |
| Botelho M.G., External Examiner to the Showa University Medical and Dental School, Tokyo Japan , External Examiner. Tokyo Japan, Showa University Medical and Dental School, 2010. |
| Bridges S.M., Botelho M.G., Dyson J.E., McGrath C.P.J., Tsang P.C.S. and Yiu C.K.Y., Blended learning and interactive pedagogies: Supporting problem-based learning in dental education, CITE Research Symposium 2010. 2010. |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Lou Y., Botelho M.G. and Darvell B.W., The Effect of Silver Diammine Fluoride on Demineralized Enamel and Dentine, European Organisation for Caries Research, Budapest, Hungary.. ORCA, 2009, 56th ORCA Congress: Abstract 89. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Researcher : Bridges SM |
| Project Title: | Investigating online learning in dentistry - an interactional approach |
| Investigator(s): | Bridges SM, Botelho MG, Dyson JE |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2008 |
| Completion Date: | 07/2009 |
| Abstract: |
| Problem-based learning (PBL) in Dentistry is a recognised approach to curriculum design that has been adopted in a variety of forms throughout the world from Sweden (Haghparast et al, 2007) to the USA (Fincham et al, 2001) to Hong Kong (Dyson et al, 2006, Samaranayake, 2007). PBL curricula reflect constructivist principles of a) collaborative learning through small group problem-solving, and b) content integration through the examination of real-life, complex problems. Prosser’s (2004) review of PBL across disciplines indicated better assessment outcomes for PBL students in clinical assessments. Research in PBL in dental education, however, has been relatively sparse in comparison with medicine. The majority of published work has focussed on issues related to pedagogy and curriculum design with few studies on student and staff perceptions of PBL in Dentistry (Haghparast et al, 2007). Prosser (2004) also noted a lack of studies on “the way students experience and understand” PBL courses (p. 57). The focus of this study will therefore be on the ‘way’ students experience and understand a facet of their PBL learning. Face-to-face training in the PBL process provides important scaffolding for learners in group problem solving. Independent learning is also a major facet of PBL and in Hong Kong the issue of supporting student learning, particularly for undergraduates making the transition from traditional secondary curricula, is a pressing one. One practical solution is to build the infrastructure for online support of independent learning. There is general consensus that online learning in Dentistry has a growing future with a recent survey of faculty administrators in the USA showing strong support in their vision and mission (Hillenburg et al, 2006). In 2005, the Faculty of Dentistry at The University of Hong Kong (HKU) began use of WebCT to support online learning in PBL. Since then, purpose-built ‘learning objects’ have been incorporated in the WebCT platform. The definition of ‘learning object’ is still relatively unstable with the broadest definition incorporating all digital resources (Liber, 2005). Churchill’s (2005) conceptualisation is useful for this study with its focus on interactivity defining ‘learning objects’ as “a mediating tool and amplifier of the learners’ physical and intellectual capacity to deal with the complexity of a learning activity” (p. 334). Therefore, the value of the incorporation of learning objects is not their novelty effect in stimulating student interest but rather their usefulness as cognitive tools to support deeper engagement and authentic learning in a constructivist framework (Herrington & Kervin, 2007). To date, the HKU Faculty of Dentistry has developed and integrated learning objects such as: a) video recordings of clinical dental procedures and b) surrogate virtual reality (VR) simulations as navigable QTVR (QuickTime VR) panoramic files. The former facilitate more detailed examination (via close up, time delay, replay functions) than possible in a live, clinical situation with associated limits on time and restricted visibility. The latter allow students to navigate study casts (synthetic models) as photographically captured panoramic learning objects. Images of the study casts are taken at multiple viewing angles and ‘stitched’ together as QTVR panoramas to create a virtual object that can be rotated and viewed from multiple angles via ‘grab’ and ‘drag’ functions. While there is consensus as to the value of incorporating web-based innovations into higher education curricula, little has been written in dental education. Recent surveys of dental curriculum managers (Andrews & Demps, 2003, Hillenburg et al, 2006, Kassebaum et al, 2004) indicated strong support with North American dental institutions reporting the most frequent innovation to be the increased use of computer and web-based learning (Kassebaum et al, 2004). This rapid growth is reflected in recent calls for guidelines for the use and quality assurance of ICTs in dental education (Nattestad et al, 2002). Studies on dental student engagement with online curriculum innovations have been scarce with two notable exceptions. Ludlow & Platin’s (2000) survey comparing dental student perceptions of learning from web page panoramas to slide tapes found a general preference for web page panoramas due to their easier use and greater flexibility. This is positive in terms of student perceptions but does not provide insights into performance. Wong et al’s (2000) study into the effectiveness of abstract animations and QTVR in teaching operative dentistry. This study used control groups to examine the impact of modalities on students’ preparation for an amalgam restoration as a summative assessment task. Findings indicated the potential for QTVR to a) increase skill acquisition and b) the quality of restorations. Foundations were laid for interactional analysis based on time on task but other interactional features were not the subject of the study. Research in curriculum innovation in dental education has relied mainly on evaluative data such as student and staff surveys with concerns expressed regarding the need to “look inside PBL programmes” (Prosser, 2004, p.52). In the field of online learning, the reliance on self-reporting data methods such as student surveys and interviews or observations in investigating student learning has been criticized for its reliance on in a ‘consumer satisfaction’ model (Levy, 2007). When investigating online curriculum innovations, methodological issues such as time delay and recall as well as subjective variables affecting participant perceptions (including the ‘novelty effect’) can limit the outcomes of research based solely on evaluative, post-performance data. Open for investigation in dental education is the collection and analysing of real-time data of student interactions in online learning. Some recent investigations into synchronous online chat in medical education (Strømsø et al, 2007) have begun to explore patterns of interaction (such as turn taking and content analysis). No such studies have been undertaken in Dentistry to date. The key objective of this project, therefore, is to examine real-time student interaction with online learning objects with the aim of providing an evidence base for analysis of student engagement and learning. This study aims to: a) build on current interest in providing online materials for dental education by providing a research base to inform the field, and b) change the research focus in online dental education from reflective evaluations after the event to examining dental students’ interactions with online learning objects in-situ. As such, it will investigate real-time, empirical data that will provide an evidence base of actual interaction in process rather than evaluative data that provides information on reported interaction. This will be achieved through the use of screen data capture software (Camtasia TM) that will record students’ online activity as they work with the video and QTVR learning objects. Screen recordings will then form a database of real-time student interaction with online learning tools that have been purpose-designed and constructed for the BDS program. Analysis of this evidence base can provide insights into dental students’ learning processes and should inform future directions in the design and development of learning objects and in the development online curriculum innovations. |
| Project Title: | Professional Interaction in Dentistry |
| Investigator(s): | Bridges SM, Botelho MG, Corbet EF, Dyson JE, McGrath CPJ, Yiu CKY |
| Department: | Dental Faculty |
| Source(s) of Funding: | Teaching Development Grants |
| Start Date: | 03/2008 |
| Completion Date: | 02/2010 |
| Abstract: |
| The Faculty of Dentistry has identified the need for: a) A review of the behavioural and social sciences aspects of the PBL problems themselves; b) The explicit teaching of effective professional interaction skills across the years of the curriculum , particularly focussing on the PBL tutorials and clinical activities; and c) The provision of in-service support to teaching staff and the development of learning materials for students in critically and productively engaging with behavioural and sociological issues. As such, the focus of this application is a) to focus on improving the behavioural and social sciences aspects of PBL as interdisciplinary inquiry and b) to anticipate the needs of the younger 2012 undergraduate student profile. In this way, the project will engage with the intent of the 4-year Undergraduate Curriculum reform reflecting a holistic approach to education. The objectives of the project are to: 1. Revise the existing behavioural and social components of problems designed for the undergraduate PBL curriculum; 2. Further develop undergraduate students' understandings of professional interactions with patients and staff in clinical settings; 3. Further encourage critical reflection by undergraduates on the behavioural and social dimensions of their role as health care professionals. |
| Project Title: | Blended learning and interactive pedagogies: Supporting problem-based learning in dental education |
| Investigator(s): | Bridges SM, Dyson JE, Tsang PCS, McGrath CPJ, Yiu CKY, Yip HK, Botelho MG |
| Department: | Dental Faculty |
| Source(s) of Funding: | Teaching Development Grants |
| Start Date: | 02/2009 |
| Completion Date: | 02/2010 |
| Abstract: |
| The aim of this project is to move from the current PBL learning model of asynchronous interaction for problem resourcing to synchronous interaction incorporated into the face-to-face tutorials (Tutorials 1 & 2 ). This moves away from a ‘digital repository’ approach where student engagement with the available technology is out-of-step with the knowledge co-construction process during tutorials towards an interactive approach for resourcing problem scenarios linked to current Web2.0 trends. Although recent Faculty initiatives have stimulated innovative resource development, the majority of the current problem stimuli are print-based. Under this project, these resources will be technologically upgraded to include interactive 3-D objects, in-house videos of clinical procedures using intra-oral cameras etc. High resolution radiographs and oral photographs will be posted in WebCT and students will be able to manipulate these objects using zoom functions etc to examine evidence in greater depth and with improved visual clarity. The objectives of the project, therefore, are to: 1. Enhance student engagement and motivation through further incorporating interactive technologies into the problem-based curriculum 2. Extend the use of real-time learning technologies 3. Integrate face-to-face and virtual modalities within the problem process In fulfilling these objectives, The Faculty sees congruence between the wider curriculum reform intent, the philosophy of the Faculty’s PBL curriculum, and current educational trends towards increased interactivity (both in face-to-face and online teaching). |
| Project Title: | 11th International Pragmatics Association Conference Multilingual clinical interactions in Dentistry – a microanalysis |
| Investigator(s): | Bridges SM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 07/2009 |
| Completion Date: | 07/2009 |
| Abstract: |
| N/A |
| Project Title: | Oral health literacy - Implications for Hong Kong's children |
| Investigator(s): | Bridges SM, Au TKF, McGrath CPJ, Wong HM, Yiu CKY |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2010 |
| Abstract: |
| 1) To develop a new conceptual framework for oral health literacy; 2) To develop new, locally-relevant instruments to assess oral health literacy in Hong Kong; 3) To describe the relationship between oral health literacy and the oral health status and management of pre-school children in Hong Kong. |
| List of Research Outputs |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience - Journal Based Learning and Fundamentals of Dental Research – a pilot programme, the 6th International Conference on PBL in Dentistry. Hong Kong, 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience-journal based learning and fundamentals of dental research-a pilot programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.26. |
| Bridges S.M., Botelho M.G., Dyson J.E., McGrath C.P.J., Tsang P.C.S. and Yiu C.K.Y., Blended learning and interactive pedagogies: Supporting problem-based learning in dental education, CITE Research Symposium 2010. 2010. |
| Bridges S.M., Case studies of good practice: The Faculty of Dentsitry approach to blended learning, Technology Enhanced Learning@HKU. 2010. |
| Bridges S.M., Celebrating the First 10 Years of PBL – The Hong Kong Experience, 6th International Conference on Problem-based Learning in Dentistry. Hong Kong, 2009. |
| Bridges S.M., Chair, U21 Dentistry Meeting, 2009. |
| Bridges S.M. and Bartlett B.J., Constructing knowledge schemas in the workplace: An exploration of the social and the cognitive, In: Hanna Pishwa, Language and Social Cognition - Expression of the Social Mind. Germany, Mouton de Gruyter, 2009, 230-258. |
| Bridges S.M., Editorial Board, Editorial Board, Journal of Investigative and Clinical Dentistry. Wiley-Blackwell, 2009. |
| Bridges S.M., Editorial Board, European Journal of Dental Education. 2010. |
| Bridges S.M., McGrath C.P.J., Wong H.M. and Au T.K.F., Exploring The Dimensions Of Oral Health Literacy. , In: Bridges, S. M., McGrath, C. P. J., Wong, H. M., & Au, T. K. , The 9th World Congress On Preventive Dentistry, Phuket, Thailand.. 2009. |
| Bridges S.M. and Poyatos Matas C., Framing multicultural capital to understand multicultural education in practice, The International Journal of Learning. Illinois, USA, Common Ground, 2009, 16 (10): 289-395. |
| Bridges S.M., Health literacy - Whose literacy?, Frontiers in Medical and Health Sciences Education - HKU 2009 Conference: Making Sense in Communication. 2009. |
| Bridges S.M., Corbet E.F., Samaranayake L.P., Dyson J.E., Leung W.K. and McGrath C.P.J., Improving programme evaluations: The effect of curriculum review, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 37. |
| Bridges S.M. and Bartlett B.J., Moving teachers: Public texts and institutional power, In: Richard Fitzgerald William Housley, Media, Policy and Interaction. Farnham, England, Ashgate, 2009, 185-203. |
| Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., Multilingual clinical interactions in Dentistry: A microanalysis, 11th International Pragmatics Conference. Australia, 2009. |
| Bridges S.M., Multilingual clinical interactions in dentistry, Griffith Institute for Educational Research, Griffith University. 2009. |
| Bridges S.M. and Lo E.C.M., PBL Programme design - past, present and future, Faculty of Education, Division of Speech & Hearing Sciences, The University of Hong Kong. 2009. |
| Bridges S.M., McGrath C.P.J., Yiu C.K.Y. and Cheng S.S., Reassuring' during clinical examinations: Novice and expert talk in dentistry, Journal of Asian Pacific Communication. John Benjamins, 2010, 20:2: 185-206. |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Poyatos Matas C. and Bridges S.M., Framing multicultural capital to understand multicultural education in practice, Sixteenth International Conference on Learning. Barcelona, Spain, 2009. |
| Yiu C.K.Y., Bridges S.M. and Cheung R.T.F., Evaluation of Medical Case-based Learning as a Dental Curriculum Enhancement Programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.21. |
| Researcher : Chai L |
| List of Research Outputs |
| Chai L., Song Y., Zee K.Y. and Leung W.K., Single nucleotide polymorphisms of complement component 5 and periodontitis, J Periodontal Res. 2010, 45(3): 301-308. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of Vascular Endothelial Growth Factor (VEGF) on Osteoblastic Cells in vitro, Houston Poster Award - the 86th Congress of the European Orthodontic Society, Portoroz, Slovenia, June 15-19, 2010. 2010. |
| Yang Y., Chin A., Tan Y.Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of VEGF and medicinal herb SM on bone cells, Honourable Mention Award of poster presentation at the 1st Hong Kong International Dental Expo and Symposium (HKIDEAS), Hong Kong, 18-20 June 2010. 2010. |
| Yang Y., Wong H.M. and Chai L., Poster Presentation: Attitudes of BDSI and BDSII Students to Self-assessment of Performance in PBL Tutorials, The 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 Nov. 2009. |
| Researcher : Chan AWK |
| Project Title: | A study to investigate the attitude and effect of magnification on the clinical performance by dental undergraduates in the University of Hong Kong |
| Investigator(s): | Chan AWK, Cheung GSP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Low Budget High Impact Programme |
| Start Date: | 11/2000 |
| Abstract: |
| To evaluate dental students' attitudes towards the use of magnification in dentistry; to investigate the effect of magnification on the quality of typical restorative procedures undertaken by dental students; to investigate the influence of the use of magnification on decision-making behaviour in restorative treatment planning. |
| Researcher : Chan CKP |
| List of Research Outputs |
| Chan C.K.P., Hands on Practice on Scaling & Root Debridement , 牙周基础治疗基本技术示范与 仿真头模训练, Worldwide Universities Network Oral Health Science, Nanjing Forum 2009. 2009. |
| Chan C.K.P., Hands on Practice on Scaling & Root Debridement, 牙周基础治疗基本技术示范与 仿真头模训练, Worldwide Universities Network Oral Health Science, Nanjing Forum 2009. 2009. |
| Researcher : Chan EKK |
| List of Research Outputs |
| Cheung L.K., Leung Y.Y., Chow L.K., Wong M.C.M., Chan E.K.K. and Fok Y.H., Incidence of neurosensory deficits and recovery after lower third molar surgery: a prospective clinical study of 4338 cases, International Journal of Oral and Maxillofacial Surgery. 2010, 39: 320-326. |
| Researcher : Chang JWW |
| Project Title: | Efficacy of root canal irrigation for debris and tissue removal |
| Investigator(s): | Chang JWW, Cheung GSP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 06/2009 |
| Abstract: |
| Purpose The purpose of this study is to examine the efficacy of various types of irrigation techniques used during endodontic treatment for remaining debris and (simulated) necrotic tissue from the root canal space. Key issues and problems addressed The process of preparing the root canal (during clinical root canal treatment) invariably produces dentinal debris that may comprise of both organic and inorganic materials. These debris have been shown to accumulate and can block the canal in due course leading to other iatrogenic errors. On the other hand the debris may get extruded; extrusion of debris into the periapical tissues can lead to inflammation, pain and delayed healing (Seltzer et al. 1968; Torneck et al. 1973; Seltzer & Naidorf 1985). The most common method of removing these dentinal debris is by flushing the canal with a solution. The use of a positive i.e injection with pressure from an irrigating syringe is a typical example. Various factors have been known to influence the efficacy of such irrigation methods in the root canal; 1) Taper of the canal (i.e. thus affecting the depth of needle penetration) (van der Sluis et al. 2005); 2) The relative diameter of the apical preparation and the size of the irrigating needle (Falk & Sedgley 2005); 3) Type of irrigating needle (Vinothkumar et al. 2007); 4) Type of irrigating solution; 5) Volume of the irrigating solution; 6) Ultrasonic activation of the irrigating solution (Ahmed et al. 1987); 7) Canal curvature (Nguy & Sedgley 2006); 8) Surface tension of the root canal walls (Giardino et al. 2006); 9)Concentration of irrigating solution (Baumgartner & Cuenin 1992). Recently, reversing the flow of the irrigating solution by introducing a partial vacuum in the root canal has been suggested (Nielsen & Baumgartner 2007). Most studies evaluated the debris removal from extracted natural teeth using digital photography of the root canal after splitting the root apart (Crumpton et al. 2005) or under SEM with a 5-step grading system (Naaman et al. 2007). In these studies, the canal dimensions and the volume of irrigating solution were standardized, but each tooth may have a different root canal anatomy and curvature which can lead to variation in the reported irrigating efficacy and debris removal thus making it difficult to correlate the results from different studies. Few have tried to standardize the size of the anatomical defect (e.g. fins) by incorporating a standardized artificial grooves in an already instrumented plastic block (Lee et al. 2004) or single-rooted teeth (van der Sluis et al. 2005). There is a lack of information on the extrusion of solution with various irrigation techniques as well as their efficacy on the removal of debris and pulp tissue within the canal. A standardized laboratory-based study is needed to compare the effect of these various techniques. |
| List of Research Outputs |
| Mahindre P.P., Cheung G.S.P. and Chang J.W.W., Micro-push-out bond strengths of an adhesively luted fiber-post subjected to simulated occlusal loads, Annual Scientific Meeting of the Faculty of Dentistry, The University of Hong Kong, Hong Kong, Nov. 2009. |
| Researcher : Chau CM |
| Project Title: | The use of magnetic resonance imaging (MRI) in daily dentistry |
| Investigator(s): | Chau CM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2008 |
| Abstract: |
| The current use of magnetic resonance imaging (MRI) in dentistry is limited to temporomandibular joint (TMJ) assessment and soft tissue tumour examination. The ability of MRI in daily dentistry such as caries detection, bone and apical lesion exploration, implant and third molar assessments, and endodontic treatment have not been fully investigated. Interproximal caries is detected by conventional intraoral radiographs. Demineralization of enamel and dentine will result in decease in x-ray attenuation and will display as a radiolucent region on a radiograph. The limitation of intraoral radiography in caries detection is low sensitivity. Radiolucent region can only be shown when more than fifty percent of enamel is demineralised. The detection of secondary caries under amalgams and crowns has been difficult due to overlap of opacity from the restorative materials in conventional radiography. There is a ground breaking potential in the use of MRI in detection of secondary caries. We hypothesize that carious demineralization could be demonstrated in MRI because of an increase in free mobile ions, including hydrogen ions. The caries could be shown as a hyperintense region on MRI images acquiring sequences such as MPRAGE (T1-weighted 3D GRE sequence) and 3D vibe (spoiled 3D GRE sequence with ultrashort TR). These sequences are made for MR angiography and they are sensitive in water content differentiation. The use of these sequences is potentially good for secondary caries detection because metallic restorative materials will not generate extensive artefact on images like conventional radiography. We already have preliminary evidences supporting our hypothesis. To the best our knowledge, no journal has been published on this area. Bone and apical lesions used to be difficult to detect on traditional MR images especially when there are dental fillings or crowns because of the appearance of artefacts. Both bone and enamel are shown as negative contrast on MRI, which are totally opposite to conventional radiography. This difference in interpretation skill makes the MRI less popular than conventional radiography in dental radiology. With the development of advanced MR imaging sequences such as MPRAGE and 3D vibe, they can overcome noises induced by dental fillings and crowns. These sequences are able to acquire high-resolution isotropic images up to 0.6mm. They also allow multiplanar reformatting, which simplifies imaging protocols and reduces acquisition time when 3D model (imaging in multiple planes) is required. We hypothesize that customization of the above mentioned MR sequence could make images that have enough diagnostic value for bone and apical lesion. Dental implant and third molar assessments are now major requests for computed tomography (CT) examinations. The essential information obtained is multiplanar images and position of vital anatomic structures such as inferior dental nerve. The nerve cannot be shown directly on CT or conventional radiograph because soft tissue is not able to absorb enough radiation in order to produce an image. We hypothesize that MRI could provide better identification on inferior dental nerve bundle because the nerve itself would alter magnetic signal to form a positive image as compared with the negative one in CT. Bone quality could be well demonstrated by analyzing cancellous bone in the jaws which again is not possible in CT. Compatible resolution for dimensional measurements can be achieved. Dental pulp imaging is possible using high resolution MRI. Dental pulp can be well differentiated from enamel and dentine because of its fluid content from blood vessel, nerve and fibroblastic tissue. Necrotic or non-vital pulp will have much less MR signals. MPRAGE and 3D vibe sequences can enhance MR signals from dental pulp. They are very sensitive to small pulpal change. This method will avoid the administration of contrast medium like other studies. Dental pulp can be investigated in a non invasive way. An animal study has been done on a 7T MRI scanner, which shows detailed morphology of the pulp. We hypothesize that freshly extracted human tooth should have adequate water content for imaging. Applying the above sequences in a high magnetic field scanner can elucidate pathological changes in human dental pulp together with morphological progress. |
| Project Title: | XIIth European Congress of Dento-maxillo Facial Radiology Technical concerns of 3D cephalometric measurement using CBCT images |
| Investigator(s): | Chau CM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2010 |
| Completion Date: | 06/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Chau C.M., Wong R.W.K. and Hagg E.U.O., 3-Dimensional Cone-Beam Computerized Tomographic Cephalometric Database on Jaw Dimensions in Chinese, The Open Anthropology Journal 2010. 2010, 3: 2-7. |
| Chau C.M., Cheung R.T.F., Jiang X., Au-Yeung P.K.M. and Li L.S.W., Acupuncture of motor-implicated acupoints on subacute stroke patients: an fMRI evaluation study, Medical Acupuncture. 2009, 21(4): 233-241. |
| Chau C.M., Cheung R.T.F., Jiang X., Au Yeung P.K.M. and Li L.S.W., An fMRI study showing effect of acupuncture in chronic stage stroke patients with aphasia, Journal of Acupuncture & Meridian Studies. 2010, 3: 53-57. |
| Chau C.M., Cheung R.T.F., Jiang X., Au-Yeung P.K.M. and Li L.S.W., Increased brain activation in motor cortex after acupuncture treatment for motor recovery in chronic stroke patients, The Open Rehabilitation Journal. 2009, 2: 89-94. |
| Chau C.M., MRI signals of dental caries, Conjoint Scientific Sessions, Faculty of Dentistry, The University of Hong Kong and Peking University, School of Stomatology, Hong Kong, China, 16 – 17 November, 2009. |
| Chau C.M., Hagg E.U.O. and Wong R.W.K., Oral Presentations: Technical Concerns of 3D Cephalometric Measurement Using CBCT Images, The XIIth European Congress of Dento-Maxillo Facial Radiology, Istanbul Turkiye, 2 – 5 June, 2010. |
| Chau C.M., Oral Radiography, HKU Space, Dental Hygenists course. 2010. |
| Chau C.M. and Wong R.W.K., Use of Cone Beam CT in Orthodontics, Oral Health and Science Seminars. 31 March. 2010. |
| Researcher : Chee C |
| List of Research Outputs |
| Chee C., Ho K.W., Yau S.W., Chu C.H. and Lo E.C.M., Oral hygiene and gingival status of Hong Kong pre-school children, Journal of Dental Research. 2009, 88 (Spec Iss B): 656 (PAPF/APR). |
| Researcher : Chen K |
| List of Research Outputs |
| Chen K., Wong R.W.K. and Rabie A.B.M., Title: LEPR is a Predisposing Factor of Mandibular Retrognathism; A SNP Association and Animal Study , GSK Outstanding Oral Presentation (Junior). Awarded by the Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. 2009. |
| Researcher : Chen L |
| Project Title: | C-fos expression and chromosomal changes in human ameloblastoma |
| Investigator(s): | Chen L, Cheung LK, Wong MP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To study the overall pattern of chromosomal alteration in multicystic and unicystic ameloblastoma by CGH and identify the important regions which may harbor critical genes involved in ameloblastoma pathogenesis. |
| Researcher : Chen Y |
| List of Research Outputs |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Antimicrobial activity of Prunes extract on Streptococcus mutans, Second Prize of E-poster Competition for Postgraduate Students. Awarded by IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. 2010. |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Poster Presentation: Antimicrobial activity of Prunes extract on Streptococcus mutans, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Researcher : Chen Z |
| List of Research Outputs |
| Pow E.H.N., Chen Z. and Leung K.C.M., Complications and their managing principles for dental implant therapy, In: Chen ZF, Research and clinical application of dental implant therapy. Beijing, People’s Military Medical Press, 2010, 235-246. |
| Researcher : Cheng SS |
| List of Research Outputs |
| Bridges S.M., McGrath C.P.J., Yiu C.K.Y. and Cheng S.S., Reassuring' during clinical examinations: Novice and expert talk in dentistry, Journal of Asian Pacific Communication. John Benjamins, 2010, 20:2: 185-206. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Researcher : Cheung BPK |
| List of Research Outputs |
| Ajonuma L.C., Lu Q., Cheung B.P.K., Leung W.K., Samaranayake L.P. and Jin L.J., Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva, Cell Biology International. 2010, 34: 147-152. |
| Researcher : Cheung GSP |
| Project Title: | Retention and leakage pattern of dentine-bonded root canal posts |
| Investigator(s): | Cheung GSP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 10/2008 |
| Abstract: |
| PURPOSE OF PROPOSED STUDY To compare the retention of fiber-post cemented with various luting systems, and to correlate this retentive strength with the marginal seal of posts made of a different material, after simulated occlusal loading. KEY ISSUES Current trends are moving towards the use of the more aesthetically pleasing fibre-reinforced resin-based post (fibre-post) systems for those teeth that have been root canal-treated [1]. These relatively flexible fibre-posts, when used to support the final crown, have been shown to produce a more favorable pattern of root fracture than the more rigid, stainless steel or nickel-chrome, posts in the event of a sudden impact to the treated tooth [2,3]. While the fibre-posts may be bonded in place with an adhesive resin cement, hence the possibility of a greater retention and reinforcing the remaining root structure than metallic posts, the marginal seal of a dentine-bonded post has been dubious due to the high C-Factor (as a result of the long, cylindrical configuration) that can prevent an effective adhesion to entire perimeter of the root canal dentine [4]. Leakage via the margin of the final restoration is an important cause of failure of endodontic treatment [5]. Thus, there is a dilemma in the choice between the more rigid metallic and the flexible fibre-posts. There is also a scarcity of report of the effect on the sealing ability of repeated occlusal loads acting on the restoration [6]. References: 1. Christensen GJ. Post concepts are changing. J Am Dent Assoc 2004; 135: 1308-10. 2. Schmitter M, Huy C, Ohlmann B, Gabbert O, Gilde H, Rammelsberg P. Fracture resistance of upper and lower incisors restored with glass fiber reinforced posts. J Endod 2006; 32: 328-30. 3. Stricker EJ, Göhring TN. Influence of different posts cores on marginal adaptation, fracture resistance, and fracture mode of composite resin crowns on human mandibular premolars. An in vitro study. J Dent 2006; 34: 326-35. 4. Tay FR, Pashley DH. Monoblocks in root canals: a hypothetical or a tangible goal. J Endod 20007; 33: 391-8. 5. Saunders WP, Saunders EM. Coronal leakage as a cause of failure in root-canal therapy: a review. Endod Dent Traumatol 1994; 10: 105-8. 6. Chang JWW. Leakage of post-endodontic restorations after simulated occlusal loading. MDS(Endodontics) Thesis, Faculty of Dentistry, The University of Hong Kong, 2006. |
| Project Title: | Development of a novel root canal post system |
| Investigator(s): | Cheung GSP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 02/2010 |
| Abstract: |
| To investigate the applicability of shape memory alloys of various formulations for the restoration of endodontically treated teeth. |
| List of Research Outputs |
| Cheung G.S.P. and Liu C.S.Y., A retrospective study of endodontic treatment outcome between nickel-titanium rotary and stainless steel hand filing techniques, Journal of Endodontics. 2009, 35: 938-943. |
| Cheung G.S.P., Cyclic fatigue of NiTi instruments, World Dental Show, Indian Dental Association, Mumbai, India, Oct. 2009. 2009. |
| Cheung G.S.P., Dental Tribune International, Journal editor of Roots: the International Magazine of Endodontics. 2010. |
| Cheung G.S.P., Evidenced based approach to root canal cleaning and shaping, F.D.I. Continuing Education Programme, Varanasi, India, Nov. 2009. 2009. |
| Cheung G.S.P., Journal editor of Endodontic Practice Today. 2010. |
| Cheung G.S.P., Journal editor of Hong Kong Dental Journal. 2010. |
| Cheung G.S.P., Journal editor of International Endodontic Journal. 2010. |
| Cheung G.S.P., Managing the diseased pulp: from diagnosis to restoration to health and function, F.D.I. Continuing Education Programme, Yangon, Myanmar, Jan. 2010. 2010. |
| Cheung G.S.P., Surgical endodontics, Invited lecture at the Guanghau School of Stomatology, Sun-yat-sen University, Guangzhou, China, Nov. 2009. 2009. |
| Cheung G.S.P., 專家視頻講座: 根管治療失敗的根尖外科處理, Clin J Stomatol Res (Electronic Edition). 2010, 4: 33. |
| Fan B., Min Y., Lu G., Yang J., Cheung G.S.P. and Gutmann J.L., Negotiation of C-shaped canal systems in mandibular second molars, Journal of Endodontics. 2009, 35: 1003-1008. |
| Kim H.C., Yum J., Hur B. and Cheung G.S.P., Cyclic fatigue and fracture characteristics of ground and twisted nickel-titanium rotary files, Journal of Endodontics. 2010, 36: 147-152. |
| Mahindre P.P., Cheung G.S.P. and Chang J.W.W., Micro-push-out bond strengths of an adhesively luted fiber-post subjected to simulated occlusal loads, Annual Scientific Meeting of the Faculty of Dentistry, The University of Hong Kong, Hong Kong, Nov. 2009. |
| Wang Y., Cheung G.S.P., Xu X., Zhao S. and Zhang C., The effect of cultured autologous periodontal ligament cells on the healing of delayed autotransplanted dog's teeth, Journal of Endodontics. 2010, 36: 264-267. |
| Yin X., Cheung G.S.P., Zhang C., Masuda Y.M., Kimura Y. and Matsumoto K., Micro-computed tomograhic comparison of nickel-titanium rotary versus traditional instruments in c-shaped root canal system, Journal of Endodontics. 2010, 36: 708-712. |
| Researcher : Cheung LK |
| Project Title: | Gene therapy for tissue-engineered craniofacial bone reconstruction |
| Investigator(s): | Cheung LK, Zheng L, Chan BP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2008 |
| Abstract: |
| Purpose: To evaluate the effectiveness of the custom-desgined collagen microspheres seeded with genetically modified bone marrow stromal cells (BMSCs) overexpressing bone morphogenetic protein (BMP)-2 in craniofacial bone reconstruciton. Key issues and problems: 1. Radical surgical resections of benign or malignant tumours create large craniofacial bony defects. Autologous bone grafts from the ribs, fibula, iliac crest or cranial vault are widely accepted surgical methods for bone reconstruction. However, both the size of the bone transplant and the additional lengthening of the operation time for autologous bone harvest increase the donor-site morbidities. 2. Efforts of using recombinant proteins such as recombinant human BMP-2 have been reported for years. One of the major problems in clinical application is the fast dissipaton rate after administeration. Thus high concentration of growth factors are usually required to achieve satisfying results. High dosages of these proteins, however, raise concerns regarding the safety as well as costs. 3. Porous block ceramics or polymers are widely used scaffolds for three dimensional cell culture. These porous scaffolds provide a suitable micro-enviroment for cells to regenerate tissue. However, vascularization and nutrition remains a problem thus limiting the size of these constructs. Hypothesis of the study: The custom-desgined collagen microspheres provide an ideal enviroment for the proliferation and differentiation of the BMSCs, and the genetically modified BMSCs overexpressing BMP-2 can enhance bone formation in bone reconstruction of critical size defects. Objectives: 1. To design and construct an adeno-associated virus (AAV) containing the human BMP-2 gene. 2. To evaluate the response of the genentically modified BMSCs overexpressing BMP-2 to the custom-designed three-dimensional bio-resorbable collagen microspheres on bone production and mineralization in vitro. 3. To assess the effectiveness of the tissue-engineered construct on reconstructing critical size mandibular defects using a rabbit model. |
| Project Title: | Can continuous distraction osteogenesis shorten the treatment time? |
| Investigator(s): | Cheung LK, Wong MCM, Zheng L |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 09/2008 |
| Abstract: |
| (1) To develop an auto-driven distraction system incorporated with a memory chip which can record the data of the distraction process; (2)to evaluate the healing process of the distraction callus at routine and rapid rates by continuous distraction using a rabbit model of mandibular lengthening; (3) to study the adaptation of the gingiva, muscles and nerves in association with distraction osteogenesis at routine and rapid rates by continuous distraction;(4) to assess the possibility of shortening the treatment course by achieving a rapid rate continuous distraction osteogenesis. |
| Project Title: | Visiting Research Professors Scheme 2009-10 |
| Investigator(s): | Cheung LK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Visiting Research Professors Scheme |
| Start Date: | 09/2009 |
| Abstract: |
| To support the appointment of Professor Herman S. Cheung as Visiting Research Professor in the Faculty of Dentistry. |
| Project Title: | Biological characterization of different sources of dental stem cells |
| Investigator(s): | Cheung LK, Ma L, Zheng L |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 03/2010 |
| Abstract: |
| Purpose: To evaluate the Biological characteristics of various sources of dental stem cells from different sources by comparing the cell markers, immunophenotype characteristics, and proliferation and pluripotent differentiation between dental pulp stem cells (DPSCs), dental follicle precursor cells (DFPCs), stem cells from human exfoliated deciduous teeth (SHEDs), stem cells from apical papilla (SCAP), and periodontal ligament stem cells (PDLSCs). Key issues and problems: 1. Adult mesenchynal stem cells (MSCs) are multipotent cells capable of giving rise to cells of mesodermal origin, including bone, cartilage, fat, tendon, muscle and blood vessel, thus making these cells promising candidates for cell-based tissue engineering for the repair of lost or damaged tissues. The sources of MSCs in adults are limited to bone marrow and adipose tissues. 2. Several sources of dental stem cells have been isolated from third molars and exfoliated teeth, including DPSCs, DFPCs, SHED, SCAP, and PDLSCs. Although studies showed that they exhibit the capacity of self renewal and multiple differentiations, their biological properties are not fully defined nor have not been properly compared among themselves. 3. Cell surface molecules play an important role in cell signaling leading to cellular proliferation and differentiation whereas the immunogenic properties define the transplantation possibility to the same donor or other hosts. Most of our understanding on the biological properties and characteristics of MSCs is limited from bone marrow stem cells. No single marker that definitively designates the MSCs in vivo has been identified. 4. Although the potential of dental stem cells in tooth tissue engineering have been explored, the data to show their capabilities on osteogenic and chondrogenic differentiation are limited. Their potential of differentiating into the other tissues, such as fat, blood vessel, muscle and nerve, remains unknown. The differentiation capacity among these dental stem cells has not been systematically compared. Hypothesis: Different sources of dental stem cells have different biological characteristics including expession of various surface markers, immunologic properties, proliferation ability, colony-forming efficiency, and differentiation ability. Objectives: 1. To evaluate and compare the cell markers and immunophenotype characteristics of different dental stem cells. 2. To evaluate and compare the proliferative capability and pluripotent differentiation of different dental stem cells. |
| List of Research Outputs |
| Cheung L.K., 3D surgical planning of patients with facial deformities, 3rd International Congress & Workshops 3D Diagnosis and Virtual Treatment Planning of Cranio-Maxillo-Facial Deformity. 2010, p 57. |
| Cheung L.K., 3D surgical planning of patients with facial deformities, 3rd International Congress & Workshops - 3D Diagnosis and Virtual Treatment Planning of Cranio-Maxillo-Facial Deformity, 25-27 March 2010, Eindhoven, Netherlands. 2010, 57. |
| Cheung L.K., A patient complains of numbness after wisdom tooth surgery: what would you do? Obstructive sleep apnoea syndrome - what is the role of the dental profession., Symposium 4 - Oral Surgery, Hong Kong International Dental Expo and Symposium, Hong Kong, June 2010. p. 66. |
| Cheung L.K. and Chua H.D.P., A top 10 cited paper (2006-2008) award from the publisher Elsevier for article "A meta-analysis of cleft maxillary osteotomy and distraction osteogenesis",, International Journal of Oral and Maxillofacial Surgery. 2009. |
| Cheung L.K., Advisory Board Member (since January 2009), Archives of Orofacial Sciences. 2010. |
| Cheung L.K., Associate Editor (since April 1999), International Journal of Oral and Maxillofacial Surgery. 2009. |
| Cheung L.K., Associate Editor for the section on Oral and Maxillofacial Surgery (since November 2009), Journal of Investigative and Clinical Dentistry (JICD). 2009. |
| Cheung L.K., Associate Editor-in-Chief, Chinese Journal of Oral Implantology. 2009. |
| Cheung L.K., Chairman, BIOMET 3i HK Symposium 2009, Hong Kong. 2009. |
| Cheung L.K., Chief Examiner of Oral and Maxillofacial Surgery (since 2004), Hong Kong Dental Council Examination. 2009. |
| Cheung L.K., Chief Examiner of Specialty Board Examination (since 2004), Hong Kong College of Dental Surgeons, Hong Kong Academy of Medicine. 2009. |
| Cheung L.K., Cleft distraction vs osteotomy. Midface augmentation - implants versus osteotomy, 2009 CUHK ENT Conference Technological Innovations in Otorhinolaryngology - Head and Neck Surgery. 2009, 49-50. |
| Cheung L.K., Dental implant rehabilitation of the atrophic mandible, 32nd Asia Pacific Dental Congress 2010, Colombo, Sri Lanka, May 2010. p. 50. |
| Cheung L.K., Chua H.D.P., Hariri F., Lo J., Ow A. and Zheng L., Distraction Osteogenesis (Chapter 48), Oral and Maxillofacial Surgery, edited by Lars Andersson, Karl-Erik Kahnberg and M. Anthony Pogrel. Wiley-Blackwell, 2010, 1027 - 1059. |
| Cheung L.K., Editor (since 1996), Stomatology Progress. 2009. |
| Cheung L.K., Editor (since 1997), Chinese Journal of Oral and Maxilloffacial Surgery. 2009. |
| Cheung L.K., Editor (since 2000), Asian Journal of Oral and Maxillofacial Surgery. 2009. |
| Cheung L.K., Editor (since 2001), Chinese Journal of Stomatological Information. 2009. |
| Cheung L.K., Editor (since 2002), West China Journal of Stomatology. 2009. |
| Cheung L.K., Editor (since 2002), Year-Book of Chinese Stomatology. 2009. |
| Cheung L.K. and Lo J., Endoscope-assisted rigid fixation for intraoral vertical subsigmoid osteotomy: a preliminary clinical study, Journal of Oral and Maxillofacial Surgery. 2010, 68: 8-14. |
| Cheung L.K., Examiner of the conjoint MOSRCS and Intermediate Examination of FCDHK (since 1998), Royal College of Surgeons of Edinburgh and College of Dental Surgeons of Hong Kong. 2009. |
| Cheung L.K., Guest Professor (from 2001), School of Stomatology, Peking University, Beijing, PR China. 2009. |
| Cheung L.K., Guest Professor (from 2005), Faculty of Stomatology, Shanghai Second Medical University, Shanghai, PR China. 2009. |
| Cheung L.K., Honorary Advisor (since 2003), Hong Kong Association of Cleft Lip and Palate. 2009. |
| Cheung L.K., Honorary Associate Professor (from 1996), China Dental Implantology Center, Ministry of Public Health, PR China. 2009. |
| Cheung L.K., Honorary Associate Professor (from 1996), Faculty of Stomatology, West China University of Medical Sciences, Chengdu, PR China. 2009. |
| Cheung L.K., Honorary Associate Professor (from 1996), School of Stomatology, Wuhan Medical University, Wuhan, PR China. 2009. |
| Cheung L.K., Honorary Consultant (April 2007 - March 2010; 1/4/2010 - 31/3/2013), Department of Oral Maxillofacial & Dental Surgery, Hong Kong West Cluster Hospitals, Hospital Authority of Hong Kong. 2009. |
| Cheung L.K., Honorary Consultant in Dentistry (April 2008 - March 2010), United Christian Hospital, Hong Kong. 2009. |
| Cheung L.K., Honorary Editorial Advisor (since 2003), Hong Kong Dental Journal. 2009. |
| Cheung L.K., Honorary Professor (from 1998), Faculty of Stomatology, Nanjing Medical University, Nanjing, PR China. 2009. |
| Cheung L.K., Honorary Professor (from 2000), Faculty of Stomatology, Fourth Military Hospital, Xian, PR China. 2009. |
| Cheung L.K., Leung Y.Y., Chow L.K., Wong M.C.M., Chan E.K.K. and Fok Y.H., Incidence of neurosensory deficits and recovery after lower third molar surgery: a prospective clinical study of 4338 cases, International Journal of Oral and Maxillofacial Surgery. 2010, 39: 320-326. |
| Cheung L.K., Invited International Editorial Board Member (since 2002), China Journal of Oral and Maxillofacial Surgery. 2009. |
| Cheung L.K., Invited Speaker, 3D surgical planning of patients with facial deformities, 3D Facial Imaging Research Group International Congress & Workshops, Eindhoven, Netherlands. 2010. |
| Cheung L.K., Invited Speaker, Management of dentofacial deformity by distraction osteogenesis, Scientific Meeting, School of Dentistry, Chonnam National University, Korea. 2009. |
| Cheung L.K., Invited Speaker, The application of surgical navigation in maxillofacial and implant dentistry, Advances and High-tech Dentistry 2009. Jointly organized by the College of Dental Surgeons of Hong Kong and Faculty of Dentistry, The University of Hong Kong. 2009. |
| Cheung L.K., Invited local speaker, The role of dental profession in management of obstructive sleep apnoea, Guangdong-Hong Kong Academic Exchange Program, Hong Kong Dental Association, Hong Kong. 2009. |
| Cheung L.K., Invited spaker, 1) A patient complains of numbness after wisdom tooth surgery: what would you do? 2) Obstructive sleep apnoea syndrome - what is the role of the dental profession?, 1st Hong Kong International Dental Expo and Symposium Hong Kong. 2010. |
| Cheung L.K., Invited speaker / moderator, 14th Dentsply Friadent World Implant Symposium, Barcelona, Spain. 2010. |
| Cheung L.K., Invited speaker, 1) Current therapies and future trends, 2) Distraction osteogenesis - principles and protocol, 3) Risk assessment and management, and 4) Corrective soft tissue flaps and grafts, FGDP (UK) Diploma in Implant Dentistry, The Royal College of Surgeons of England, COHORT 8, Leeds, United Kingdon. 2010. |
| Cheung L.K., Invited speaker, 1) Minor oral surgery: a patient complains of numbness after wisdom tooth surgery: what would you do? 2) Dental implant rehabilitation of the atrophic mandible and 3) Risk management in dental implant treatment, 32th Asia Pacific Dental Congress, Colombo, Sri Lanka. 2010. |
| Cheung L.K., Invited speaker, A patient complains of numbness following wisdom tooth removal - what would you do?, 2010 Spring Scientific Meeting. The College of Dental Surgeons of Hong Kong. Hong Kong Academy of Medicine. 2010. |
| Cheung L.K., Invited speaker, Advances in distraction osteogenesis for oro-facial deformities, 50th Anniversary of Philippine College of Oral & Maxillofacial Surgeons, Manila, Philippines. 2010. |
| Cheung L.K., Invited speaker, Cleft distraction vs osteotomy; Midfacial augmentation - implant versus osteotomy, Conjoint Meeting of the Chinese University of Hong Kong ENT Conference & 26th Congress of the Pan Pacific Surgical Association, Hong Kong. 2009. |
| Cheung L.K., Invited speaker, Implication of the latest prophylactic regimes for the prevention of infective endocarditis on dentistry, Dental Library, HKU fund raising lecture, University of Hong Kong. 2010. |
| Cheung L.K., Invited speaker, Micrognathia with congenital bilateral anklyosis: how can we do better; Le Fort I with and without distraction: what is the more stable?, International Society of Craniofacial Surgery - XIII Biennial International Congress and Paris Distraction Symposium 2009, Oxford, UK. 2009. |
| Cheung L.K., Joint Organizer & Invited Speaker, Functional reconstruction with dental implants; Dental implant rehabilitation in atrophic jaws; Joint implant symposium, Philippine Academy of Implant Dentistry and Oral & Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Hong Kong. 2009. |
| Cheung L.K., Keynote Speaker, Distraction osteogenesis - from basic science to clinical applications, 2009 Conjoint Scientific Sessions. Faculty of Dentistry, University of Hong Kong - Peking University School of Stomatology.. 2009. |
| Cheung L.K., Keynote speaker, Mandibular distraction; History & development choices of distractors; Stereo model technology; Craniofacial distraction, Synthes distraction observership course, Hong Kong. 2009. |
| Cheung L.K., Management of dentofacial deformity by distraction osteogenesis, 2009 International Symposium "Current Trends in Dental Sciences" School of Dentistry, Chonnam National University, Gwangju, Korea Session IV Dental Deformity. 2009, 16. |
| Cheung L.K., Member of Advisory Board in Oral & Maxillofacial Surgery (since October 2009), Egyptian Journal of Oral & Maxillofacial Surgery. 2009. |
| Cheung L.K., Member of Editorial Board (since June 2008), International Journal of Dentistry . 2009. |
| Cheung L.K., Member of Education and Accreditation Committee (since November 2009), Dental Council of Hong Kong. 2009. |
| Cheung L.K., Member of editorial board (since January 2008), Oral Disease. 2010. |
| Cheung L.K., Member of the International Advisory Board, The Surgeon (since June 2005), Journal of the Royal College of Surgeons of Edinburgh and Ireland. 2009. |
| Cheung L.K., Oral & Maxillofacial Trauma, First Aid M, Hong Kong Red Cross. 2009, Chapter 8: 105-107 & 141-142. |
| Cheung L.K., Reviewer (since March 2006), Journal of Biomechanics. 2009. |
| Cheung L.K., Risk management in dental implant treatment, 32rd Asia Pacific Dental Congress 2010, Colombo, Sri Lanka. 2010, p. 49. |
| Cheung L.K., Ow T.-.C.A. and Chua H.D.P., Simultaneous modified oblique Le Fort III and segmentalized Le Fort I osteotomies, J Oral Maxillofac Surg. 2010, 68: 915-23. |
| Cheung L.K., Ow A.T.C. and Chua H.D.P., Simultaneous modified oblique Le Fort III and segmentalized Le Fort I osteotomies, Journal of Oral and Maxillofacial Surgery. 2010, 68: 915 - 923. |
| Cheung L.K., Special Invited Editor (since 2003), Chinese Journal of Craniomaxillofacial Surgery. 2009. |
| Cheung L.K., Special Invited Editor (since 2003), Chinese Journal of Stomatological Research. 2009. |
| Cheung L.K., Symposium on Minor Oral Surgery: A patient complains of numbness after wisdom tooth surgery: what would you do?, 32nd Asia Pacific Dental Congress 2010, Colombo, Sri Lanka, May 2010. p. 71. |
| Cheung L.K., Training Program Supervisor in Oral & Maxillofacial Surgery (Since December 2009), United Christian Hospital, Hong Kong. 2009. |
| Cheung L.K., Visiting Associate Professor (from 1996), Faculty of Stomatology, Zhejiang Medical University, Hangzhou, PR China. 2009. |
| Cheung L.K., Visiting Professor (November 2009 - October 2010), Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, University of Malaya, Malaysia. 2009. |
| Cheung L.K., Visiting Professor (since 1997), Shanghai Tiedao University, Shanghai, PR China. 2009. |
| Cheung L.K., Visiting Professor (since April 2004), Oral and Maxillofacial Surgery Unit, University of Adelaide, Adelaide, South Australia 5005, Australia. 2009. |
| Chua H.D.P., Bendeus S.A.M. and Cheung L.K., Cleft maxillary distraction versus orthognathic surgery – which one is more stable in 5 years?, Oral Surg, Oral Med, Oral Pathol, Oral Rad and Endod . 2010, 109: 803-14. |
| Chua H.D.P., Whitehill T.L., Samman N. and Cheung L.K., Maxillary distraction versus orthognathic surgery in cleft lip and palate patients: Effects on speech and velopharyngeal function , Int J Oral Maxillofac Surg. 2010, 39: 633-40. |
| Chua H.D.P. and Cheung L.K., The Roles of dental professionals in the management of obstructive sleep apnoea, The Hong Kong Medical Diary. 2010, 15 (3): 4-6. |
| Jayaratne Y.S.N., Lo J., Zwahlen R.A. and Cheung L.K., 3-D Photogrammetry for Surgical Planning of Tissue Expansion in Hemifacial Microsomia., Head Neck. PMID 19862829, 2009. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Computer Aided Maxillofacial Surgery: An Update (accepted), Surgical Innovations. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A. and Cheung L.K., Three-dimensional anthropometric analysis of the Chinese nose, J Plast Reconstr Aesthet Surg. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Three-dimensional color maps: a novel tool for assessing craniofacial changes., Surg Innov. 2010. |
| Ke J., Cheung L.K. and Zheng L., The best poster award at the 3rd Hiroshima Conference, Hiroshima University. 2009. |
| Leung Y.Y. and Cheung L.K., New approach in extraction of impacted wisdom teeth, The Hong Kong Medical Diary. 2010, 15 (3): 8-9. |
| Leung Y.Y. and Cheung L.K., Safety of coronectomy versus excision of wisdom teeth: a randomized controlled trial, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2009, 108(6): 821-7. |
| Lo J., Fang D.T.S. and Cheung L.K., Long term Clinical Analysis of Immediate Implant in Aesthetic Zone, Annual Meeting of Philippine Academy of Implant Dentistry. 2009. |
| Lo J., Xia J.J., Zwahlen R.A. and Cheung L.K., Surgical navigation in correction of hemimandibular hyperplasia: a new treatment strategy, J Oral Maxillofac Surg. 2010, 68: 1444-50. |
| Lo J., Yau Y.Y. and Cheung L.K., The use of skeletal of skeletal scintigraphy in the assessment of mandibular asymmetry, Conjoint Scientific Meeting of HKU and Peking University . 2009. |
| Ma L., Zheng L., Sham M.H. and Cheung L.K., Effect of nicotine on gene expression of angiogenic and osteogenic factors in a rabbit model of bone regeneration, Journal of Oral and Maxillofacial Surgery. 2010, 68: 777 - 781. |
| Ma L., Sham M.H., Zheng L. and Cheung L.K., Influence of low dose nicotine on bone healing. , J Trauma . 2010, In press. |
| Ma L., Zheng L., Sham M.H. and Cheung L.K., Uncoupled angiogenesis and osteogenesis in nicotine compromised bone healing, J Bone Miner Res . 2010, 25: 1305-13. |
| Ow A... and Cheung L.K., Bilateral sagittal split osteotomies and mandibular distraction osteogenesis: a randomized controlled trial comparintg skeletal stability, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2010, 109 (1): 17-23. |
| Ow T.-.C.A. and Cheung L.K., Skeletal stability and complications of bilateral sagittal split osteotomies and mandibular distraction osteogenesis: an evidence based review, International Journal of Oral and Maxillofacial Surgery. 2009, 67: 2344 - 2353. |
| Tam C.K., Luk W.K., Pow E.H.N., Lo J. and Cheung L.K., Immediate Implant-Supported Auricular Prosthesis Using Surgical Navigation and CAD/CAM Technology - An In Vitro Trial, 9th Meeting of International Congress on Maxillofacial Rehabilitation. 2010. |
| Zhang W., Zheng L. and Cheung L.K., Bone regeneration following radiotherapy: an animal model, J Oral Maxillofacial Surg . 2010, In press. |
| Zheng L., Ma L. and Cheung L.K., Angiogenesis is enhanced by continuous traction in rabbit mandibular distraction osteogenesis, Journal of Cranio-Maxillofacial Surgery. 2009, 37(7): p405-11. |
| Zheng L., Ma L. and Cheung L.K., Angiogenesis is enhanced by high frequency traction during distraction osteogenesis, J Craniomaxillofac Surg. 2009, 37: 405-11. |
| Zheng L., Ma L., Shi X. and Cheung L.K., Comparison of distraction osteogenesis versus costochondral graft in reconstruction of tempormandibular joint condylectomy with disc preservation, J Oral Maxillofac Surg . 2010, In press. |
| Zheng L., Ma L. and Cheung L.K., Comparison of gene expression of osteogenic factors between continuous and intermittent distraction osteogenesis in rabbit mandibular lengthening, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2009, 108 (4): 496-9. |
| Zheng L. and Cheung L.K., Stem cells in dentistry, Stem Cell and Regenerative Medicine by Herman S. Cheung (Ed.). Bentham Science Publishers Ltd, 2010, Chapter 10: 176-186. |
| Zheng L., Hua H. and Cheung L.K., Traditional Chinese medicine and oral diseases: today and tomorrow., Oral Dis . 2010, In press. |
| Zwahlen R.A., Cheung L.K., Zheng L., Chow R.L.K., Li T., Schuknecht B., Grätz K.W. and Weber F.E., Comparison of two resorbable membrane systems in bone regeneration after removal of wisdom teeth: a randomized-controlled clinical pilot study, Clinical Oral Implants Research. 2009, 20(10): 1084-91. |
| Researcher : Chiu WK |
| Project Title: | Pregabalin versus Carbamazepine for Patients with Neuropathic Orofacial Pain |
| Investigator(s): | Chiu WK, Cheung CW, Irwin MG |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2006 |
| Abstract: |
| Neuropathic orofacial pain, particularly trigeminal neuralgia is a common and extremely unpleasant and debilitating condition which can severely affect sufferers quaity of life. Carbamazepine is an anticonvulsant blocking sodium channels and is currently the standard treatment for patients with neuropathic orofacial pain. However, its use is frequently limited by side effects such as dizziness, ataxia, marrow suppression, hypersensitivity reaction and liver dysfunction. These are potentially dangerous and close monitoring of complete blood picture, liver function and renal function are essential. Pregabalin (S-enantiomer of 3-aminomethyl-hexanoic acid) is a structural analogue of gamma-aminobutyric acid (GABA) but is functionally unrelated to it. It acts as a ligand at the alpha-2-delta binding site associated with voltage-gated calcium channels in the central nervous system. It is pharmacologically similar to gabapentin which is now well established in the treatment of neuropathic pain. However, it has a superior pharmacological profile: more predictable response with linear pharmacokinetic, higher oral bioavailability, fast onset, lack of drug interactions and better tolerability. Pregabalin has been shown to be effective in the treatment of diabetic neuropathy and post-herpetic neuralgia, and as well been used to treat epilepsy, neuropathic pain and anxiety. It is generally much well tolerated than carbamazepine. Objectives: 1. To evaluate and compare the efficacy of pregabalin and carbamazepine in patients with neuropathic orofacial pain 2. To evaluate and compare the side effects and tolerability of pregabalin and carbamazepine in patients with neuropathic orofacial pain 3. To evaluate the effects of pregabalin and carbamazepine on mood and sleep and quality of life of patients |
| Researcher : Choi MY |
| List of Research Outputs |
| Choi M.Y., Wang Y. and Watt R.M., Versatile Enzymatic System For The Production Of Guanosine Polyphosphates, Society for General Microbiology Spring Conference, Edinburgh. 2010, ED02/20. |
| Researcher : Choi WWS |
| List of Research Outputs |
| Choi W.W.S., Lee S..., McGrath C.P.J. and Samman N., Change in quality of life after combined orthodontic-surgical treatment of dentofacial deformities, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2010, 109 (1): 46-51. |
| Researcher : Chow RLK |
| List of Research Outputs |
| Zwahlen R.A., Cheung L.K., Zheng L., Chow R.L.K., Li T., Schuknecht B., Grätz K.W. and Weber F.E., Comparison of two resorbable membrane systems in bone regeneration after removal of wisdom teeth: a randomized-controlled clinical pilot study, Clinical Oral Implants Research. 2009, 20(10): 1084-91. |
| Researcher : Chow TW |
| Project Title: | Oral rehabilitation of edentulous patients with osseointegrated implant-supported complete dentures |
| Investigator(s): | Chow TW, Chu FCS, Cheung LK, Chai JYC |
| Department: | Dental Faculty |
| Source(s) of Funding: | S.K. Yee Medical Foundation - General Award |
| Start Date: | 04/2007 |
| Abstract: |
| To improve the masticatory function, nutritional status and quality of life of poor edentulous patients with unsatisfactory dentures by the provision of implant-supported complete mandibular complete dentures. |
| List of Research Outputs |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Siu S.C., Chu F.C.S., Li T.K.L., Chow T.W. and Deng F., Imaging modalities for preoperative assessment in dental implant therapy: an overview, Hong Kong Dental Journal. 2010, 7(1): 23-30. |
| Researcher : Chu CH |
| Project Title: | Effects of silver diamine fluoride on enamel and dentin with caries-like lesions against biofilm challenge |
| Investigator(s): | Chu CH, Lo ECM, Yip HK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2008 |
| Completion Date: | 03/2010 |
| Abstract: |
| Purpose The purpose of this basic research is to study the effects of SDF on mineral content of demineralised enamel and dentin incubated with an oral biofilm generated in an artificial mouth. Hypotheses 1. Silver diamine fluoride has no effect on the change in mineral content in enamel under the oral biofilm generated in an artificial mouth. 2. Silver diamine fluoride has no effect on the change in mineral content in dentin under the oral biofilm generated in an artificial mouth. Keys issues and problem addressed In the mouth, there is always a dynamic process of demineralisation and remineralisation of the exposed teeth. Caries develops when the balance tips to demineralisation; but it can be arrested when the lesion is overwhelmed by remineralisation. Results from clinical trials show that silver diamine fluoride (SDF) has an effect on caries arrest and rehardening of carious lesions (Chu et al., 2002). In vitro studies also found treatment with SDF and potassium iodide (KI) on dentin reduced caries development and inhibited surface biofilm formation (Knight et al., 2007). In addition SDF and KI treatment was found to reduce the permeability of demineralized dentin to Streptococcus mutans (Knight et al., 2005). Various research techniques have been successfully used to investigate the level of mineralization in dental hard tissue (Yip et al., 1995). Microradiography has been used in studies on mineralization of tooth tissue (Yip et al., 2007). X-ray microanalysis (XMA) is another common method to study the dental hard tissue and a variant of the method of XMA is energy-dispersive analysis (EDS). EDS has been used to study the decalcified regions of carious dentin lesions and has shown that there was a reduction in the concentrations of Ca, P, Mg and Cl, usually accompanied by an increase in concentrations of S and Zn (Sakoolnamarka et al., 2005). The level of calcium can be used as an indicator of the level of mineralization in dentin (Figures et al., 1990). In texts on cariology, data on the elemental concentration in dentin usually relates to whole dentin, and in fact the range of concentrations within any one sample is quite wide. The EDS technique allows the analysis of minute areas of highly differing structures, and may also allow the study of the variation in concentration of an element within a very small area of a specimen using internal controls. Yip and his co-workers (1995) used the EDS technique to measure the level of mineralization of dentin and reported that there was only a slight difference between the superficial dentin on the floor of a cavity where the carious tissues had been removed and the underlying sound dentin. Deeply stained and shiny lesion which is hard on probing can be observed in arrested carious lesions. A pilot test on the micro-hardness of such lesion showed there might be a change in mineralization (Chu and Lo, 2007). Caries arrest should be the result of remineralization; however, in vitro studies are few and inconsistent in supporting remineralisation effect of SDF on dental tissue. A study on sound enamel block did not find significant changes in fluoride concentration and increase in micro-hardness (Delbem et al., 2006). There is a lack of information on the mineral content of carious dentin of carious dentin lesion after SDF treatment. A laboratory based study is needed to examine the effect of SDF on mineral content of caries-like enamel and dentin which is vital in elucidating the mechanism of action of SDF in achieving the clinical results seen in our earlier studies (Chu et al, 2002). |
| Project Title: | Outreach dental caries control service for kindergarten children |
| Investigator(s): | Chu CH, Lo ECM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Health Care and Promotion Fund - Research Grant Projects |
| Start Date: | 12/2008 |
| Abstract: |
| L |
| Project Title: | Oral health status and oral features of Chinese people with Systemic Sclerosis (Scleroderma) in Hong Kong |
| Investigator(s): | Chu CH, Leung WK, Mok TMY |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2008 |
| Abstract: |
| 1.Preamble Systemic Sclerosis (SSc) is a clinically heterogeneous, systemic disorder which affects the connective tissue of the skin, internal organs and the walls of blood vessels. Scleroderma is a common symptom of people with SSc. (Scully and Cawson, 1998) People with SSc also have a high prevalence of periodontal disease (Raynaud’s & Scleroderma Association, 2008). Constriction of the oral orifice is a common finding. It can cause progressively limited mouth opening which is termed a fish-mouth. Clinical findings such as widening of periodontal spaces (Rout et al. 1996), abnormalities in periodontal microcirculation (Scardina et al., 2005), loss of gingival attachment and prominent buccal mucosal crenations (Eversole et al., 1984) were reported. Other common findings including loss of tongue mobility with fibrotic indurations (Eversole et al., 1984), dry mouth (salivary hypofunction) and telangiectasia (Nagy et al., 1994) were also mentioned in individual reports. People with SSc are at risk of dental caries, oral ulcers and fungal infections (Raynaud’s & Scleroderma Association, 2008). Erosion of facial bones may be found. Individual studies and case reports have shown osseous resorption at mandibular angles, coronoid processes and zygomatic arches (Rout et al., 1996). A Medline search of the literature was performed on July 31, 2008 and yielded no English articles published in the period of 1980 to 2008 bearing keywords of “Systemic Sclerosis” or “scleroderma” and “dental” or “oral features”, this study aims to study the oral health status and oral features of Chinese people with SSc. 2.Aims This study aims to study the oral health status, oral features and salivary function of Chinese people with SSc. 3. References 1. Eversole LR, Jacobsen PL, Stone CE (1984). Oral and gingival changes in Systemic Sclerosis (scleroderma). J Periodontol; 55:175-178. 2. Nagy G, Kovács J, Zeher M, Czirják L (1994). Analysis of the oral manifestations of Systemic Sclerosis. Oral Surg Oral Med Oral Pathol; 77:141-146. 3. Raynaud’s & Scleroderma Association. Oral and Dental Aspects of Scleroderma. Cheshire: Raynaud’s & Scleroderma Association, 2008. 4. Rout PG, Hamburger J, Potts AJ (1996). Orofacial radiological manifestations of Systemic Sclerosis. Dentomaxillofac Radiol; 25:193-196. 5. Scardina GA, Pizzigatti ME, Messina P (2005). Periodontal Microciculatory Abnormaolities in Patients with Systemic Sclerosis. J Periodontol; 76:1991-1995. 6. Scully C, Cawson RA. Medical Problems in Dentistry. New York: Churchill Livingstone, 1998. |
| Project Title: | 39th Annual Meeting of the AADR Oral health of children in rural area of the Philippines |
| Investigator(s): | Chu CH |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 03/2010 |
| Completion Date: | 03/2010 |
| Abstract: |
| N/A |
| Project Title: | Clinical evaluation of single versus multiple visit endodontic therapy |
| Investigator(s): | Chu CH, Zhang C, Lo ECM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 04/2010 |
| Abstract: |
| Preamble Despite clinical case reports were published on the success of single appointment root canal therapy, surveys found many dentists still perform root canal treatment in a conventional way i.e. multiple visits. There are numerous advantages to completing root canal therapy in a single appointment such as no risk of flare-up induced by leakage of the temporary seal between appointments and materials needed for separate visits are saved (Wahl, 1996). However, patients undergoing a single visit may experience a higher frequency of swelling and are more likely to take painkillers (Figini et al., 2007). The introduction of magnifying loupes in endodontic treatment enhances the visualisation of the surgical field. Hence, it is considered using magnifying loupes in endodontic treatment will improve clinical and radiographic outcomes. However, Del Fabbro et al (2009) reviewed the literature and found clinical trial is necessary to substantiate this affirmation. Furthermore, it may take longer time to perform the operative procedures when magnifying loupes are used. Aim The aim of this prospective clinical trial is to compare the outcome of endodontic treatment with single or multiple visit endodontic therapy. Objectives The specific objectives are firstly, to study the clinical success in single and multiple visit endodontic therapy; and secondly to compare the time used in single and multiple visit endodontic therapy with or without magnifying loupes. References 1. Wahl MJ. Myths of single-visit endodontics. Gen Dent 1996; 2: 126-130. 2. Figini L, Lodi G, Gorni F, Gagliani M. Single versus multiple visits for endodontic treatment of permanent teeth. Cochrane Database Syst Rev. 2007 Oct 17;(4):CD005296. 3. Del Fabbro M, Taschieri S, Lodi G, Banfi G, Weinstein RL. Magnification devices for endodontic therapy. Cochrane Database Syst Rev. 2009 Jul 8;(3):CD005969. |
| List of Research Outputs |
| Chee C., Ho K.W., Yau S.W., Chu C.H. and Lo E.C.M., Oral hygiene and gingival status of Hong Kong pre-school children, Journal of Dental Research. 2009, 88 (Spec Iss B): 656 (PAPF/APR). |
| Chu C.H., Clinical Trial of 8% Arginine Containing Desensitizing Paste in Hong Kong, Abstracts and Proceedings of the First Hong Kong International Dental Expo and Symposium. Hong Kong SAR, China, Hong Kong Dental Association, 2010, 59. |
| Chu C.H., Clinical Trial of 8% Arginine Containing Desensitizing Paste in Hong Kong, First Hong Kong International Dental Expo and Symposium, 2010, Hong Kong, China. 2010. |
| Chu C.H., Lo E.C.M. and You D.S.H., Clinical diagnosis of fissure caries with conventional and laser-induced fluorescence techniques, Lasers Medical Science. 2010, 25: 355-362. |
| Chu C.H., Clinical, Cosmetic and Investigational Dentistry, Editor. 2010. |
| Chu C.H. and Lo E.C.M., Dentin hypersensitivity: a review, Hong Kong Dental Journal. 2010, 7: 15-22. |
| Chu C.H., Pang K.K.L. and Lo E.C.M., Dietary behavior and knowledge of dental erosion among Chinese adults, BMC Oral Health. 2010, 10: 13. |
| Chu C.H., Lau K.P., Lui K.S., Kwok C.M. and Huang T., Effects of 8% arginine-calcium carbonate desensitizing paste on hypersensitivity teeth [Abstract], Journal of Dental Research in IADR 88th General Session 2009. 2010, 89 (Spec Iss B): 442. |
| Chu C.H., Lui K.S., Woo K. and Huang T., Effects of 8% arginine-calcium carbonate desensitizing paste on hypersensitivity teeth, In programme book of FDI Annual World Congress 2010: Invited Poster #442. 2010. |
| Chu C.H., Mei L., Lo E.C.M., Seneviratne C.J. and Yip H.K., Effects of silver diamine fluoride on mono-species cariogenic biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 363 (PAPF/APR). |
| Chu C.H., Home Remedies for Dentin Hypersensitivity, , Oral Care Link,. 2010, 1. |
| Chu C.H., Management of dentine hypersensitivity, The Hong Kong Medical Diary 2010. 2010, 15: 22-24. |
| Chu C.H., Oral Health and dental Care in Hong Kong. , In Abstracts of papers. Inaugural meeting of Family Dentistry in Taiwan, Mainland and Hong Kong 2009, Taipei, Taiwan, 2009. . 2009, 42-43. |
| Chu C.H., Chan Y.C., Cheung W.P., Ho T.K., Lau C.K., Mak K.M., Ng A., Tam A.H.S. and Woo T.C.H., Oral health status of Chinese elderly people with dementia in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 166: 1-52. |
| Chu C.H. and Lo E.C.M., Outreach caries control service for kindergarten children in Hong Kong, In: Programme book of Health Research Symposium 2010: Improving Health and Recognising Excellence. 2010, Ab01: p75. |
| Chu C.H. and Lo E.C.M., Outreach caries control service for kindergarten children in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss C): 47 (WCPD). |
| Chu C.H., Mei L. and Lo E.C.M., Use of fluorides in dental caries management, General Dentistry. 2010, 58: 37-43. |
| Gao X., Seneviratne C.J., Lo E.C.M., Chu C.H. and Samaranayake L.P., Novel and conventional assays for assessing risk of dental caries, In: Programme book of Health Research Symposium 2010: Improving Health and Recognising Excellence. 2010, Ab56: p75. |
| Ho P.L., Liu H.N., Lam C., Chu C.H. and Lo E.C.M., Occlusal featurs of primary dentition in Chinese preschool children, Journal of Dental Research. 2009, 88 (Spec Iss B): 601 (PAPF/APR). |
| Lam A. and Chu C.H., Oral health of children in rural area of the Philippines, Journal of Dental Research. 2010, 89 (Spec Iss A): 1270 in AADR 39th Annual Meeting. |
| Leung W.K., Chu C.H., Mok T.M.Y. and Ng S.K.S., Periodontal status of adults with systemic sclerosis in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 655 (PAPF/APR). |
| Lo E.C.M., Xiao Y., Chu C.H. and Liang R., Preventing fissure caries by sealants and fluorides - 30-month results, Journal of Dental Research. 2009, 88 (Spec Iss C): 127 (WCPD). |
| Lui W.Y., Sum C.K., Chan H.W.L., Chu C.H. and Lo E.C.M., Dental caries status of preschool children in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 1 (PAPF/APR). |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Action of Silver Diamine Fluoride on Dentin Caries [Abstract], First Hong Kong International Dental Expo and Symposium; Hong Kong Dental Association. 2010, In Abstracts of papers: 84. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Effects of multi-species biofilm dentin caries-like lesions in artificial mouth, Journal of Dental Research. 2010, 89 (Spec Iss A): 1393 in AADR 39th Annual Meeting. |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., Effects of silver-diamine fluoride on caries-like lesion and cariogenic biofilm, Journal of Dental Research. 2009, 88 (Spec Iss B): 40 (PAPF/APR). |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., International Association for Dental Research / Unilever Hatton Travel Award for M.Lei (co-author), 23nd Annual Scientific Meeting, Southeast Asian Division of International Association for Dental Research 2009. . 2009. |
| Wu D., Lo E.C.M., Zhang Y. and Chu C.H., Oral health and hygiene status of Hong Kong kindergarten children, Journal of Dental Research. 2009, 88 (Spec Iss B): 310 (PAPF/APR). |
| Researcher : Chu FCS |
| Project Title: | A microbiological study of bone collected during immediate dental implant surgery |
| Investigator(s): | Chu FCS, Tsang PCS, Chow TW |
| Department: | Dental Faculty |
| Source(s) of Funding: | Low Budget High Impact Programme |
| Start Date: | 11/2001 |
| Abstract: |
| To compare the microbial contamination of bone collected from surgical sites with at least healing period of four weeks, and from fresh extraction sockets prepared for dental implants; to determine if bone grafts have to be collected from areas other than fresh extraction sockets; to identify if particular pre-extraction conditions. |
| List of Research Outputs |
| Chu F.C.S., Member, Scientific Advisory Committee, Journal of Endodontics. 2009. |
| Siu S.C., Chu F.C.S., Li T.K.L., Chow T.W. and Deng F., Imaging modalities for preoperative assessment in dental implant therapy: an overview, Hong Kong Dental Journal. 2010, 7(1): 23-30. |
| Researcher : Chua HDP |
| List of Research Outputs |
| Cheung L.K. and Chua H.D.P., A top 10 cited paper (2006-2008) award from the publisher Elsevier for article "A meta-analysis of cleft maxillary osteotomy and distraction osteogenesis",, International Journal of Oral and Maxillofacial Surgery. 2009. |
| Cheung L.K., Chua H.D.P., Hariri F., Lo J., Ow A. and Zheng L., Distraction Osteogenesis (Chapter 48), Oral and Maxillofacial Surgery, edited by Lars Andersson, Karl-Erik Kahnberg and M. Anthony Pogrel. Wiley-Blackwell, 2010, 1027 - 1059. |
| Cheung L.K., Ow T.-.C.A. and Chua H.D.P., Simultaneous modified oblique Le Fort III and segmentalized Le Fort I osteotomies, J Oral Maxillofac Surg. 2010, 68: 915-23. |
| Cheung L.K., Ow A.T.C. and Chua H.D.P., Simultaneous modified oblique Le Fort III and segmentalized Le Fort I osteotomies, Journal of Oral and Maxillofacial Surgery. 2010, 68: 915 - 923. |
| Chua H.D.P., Bendeus S.A.M. and Cheung L.K., Cleft maxillary distraction versus orthognathic surgery – which one is more stable in 5 years?, Oral Surg, Oral Med, Oral Pathol, Oral Rad and Endod . 2010, 109: 803-14. |
| Chua H.D.P., Distraction osteogenesis versus orthognathic surgery - which is better for cleft lip and palate patients? (PhD Thesis, 2009). 2009. |
| Chua H.D.P., Whitehill T.L., Samman N. and Cheung L.K., Maxillary distraction versus orthognathic surgery in cleft lip and palate patients: Effects on speech and velopharyngeal function , Int J Oral Maxillofac Surg. 2010, 39: 633-40. |
| Chua H.D.P., Orthognathic surgery versus Distraction osteogenesis, 12th Postgraduate Course, FEU-NRMF Medical Center, Dept of Otolaryngology- Head and Neck Surgery . 2010. |
| Chua H.D.P. and Cheung L.K., The Roles of dental professionals in the management of obstructive sleep apnoea, The Hong Kong Medical Diary. 2010, 15 (3): 4-6. |
| Researcher : Corbet EF |
| Project Title: | Effects of periodontal therapy on the control of hypertension |
| Investigator(s): | Corbet EF, Lai SML |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 02/2009 |
| Abstract: |
| To investigate the effects of periodontal therapy on the control of essential hypertension To compare the periodontal status of patients with essential hypertension receiving intensive periodontal care at a University clinic with those receiving routine community-based dental care To determine risk factors contributing to the development of gingival enlargements in patients taking antihypertensive drugs |
| List of Research Outputs |
| Bridges S.M., Corbet E.F., Samaranayake L.P., Dyson J.E., Leung W.K. and McGrath C.P.J., Improving programme evaluations: The effect of curriculum review, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 37. |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Corbet E.F., Editorial Board, Clinical Oral Implants Research. 2009. |
| Corbet E.F., Editorial Board, Clinical Oral Implants Research. 2010. |
| Corbet E.F., Editorial Board, European Journal of Dental Education. 2009. |
| Corbet E.F., Editorial Board, European Journal of Dental Education. 2010. |
| Corbet E.F., Editorial Board, Journal of Periodontal Research. 2009. |
| Corbet E.F., Editorial Board, Journal of Periodontal Research. 2010. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Corbet E.F., Honorary Editorial Advisor, Hong Kong Dental Journal. 2009. |
| Corbet E.F., Honorary Editorial Advisor, Hong Kong Dental Journal. 2010. |
| Corbet E.F., Invited lecture, "Crown lengthening surgery", Kuwait University, January 20. 2010. |
| Corbet E.F., Invited lecture, "Imaging in Periodontology", Philippines Dental Association, Manila, Philippines, September 27. 2009. |
| Corbet E.F., Invited lecture, "Innovations in mechanical non-surgical periodontal therapy", Spring Scientific Meeting, College of Dental Surgeons of Hong Kong, May 23. 2010. |
| Corbet E.F., Invited lecture, "Innovations in mechanical non-surgical periodontal treatment", Faculty of Dentistry, University of Malaya, May 3. 2010. |
| Corbet E.F., Invited lecture, "Innovations in nonsurgical periodontal treatment", The 1st China International Symposium on Periodontology & Implant Dentistry, Hangzhou, China, May 7. 2010. |
| Corbet E.F., Invited lecture, "Periodontics - beyond the pocket, and now totally lost", 8th Asian Pacific Society of Periodontology Meeting, Singapore, August 29-30. 2009. |
| Corbet E.F., Invited lecture, "Periodontology Today", Dentistry Today - The Current Status, The Faculty of Dentistry, Royal College of Surgeons in Ireland, Dublin, Ireland, October 30. 2009. |
| Corbet E.F., Invited lecture, “Periodontal tissue management to facilitate restorative eentistry”, Continuing Education Programme, FDI World Dental Federation, Myanmar January 28. 2010. |
| Corbet E.F., PBL implementation at The University of Hong Kong, 6th International Conference on PBL in Dentistry, Hong Kong, November 13-15. 2009, 15. |
| Corbet E.F., Periodontology beyond the pocket: Now totally lost, In: P.M. Bartold, K.M. Chung, Proceedings of the 8th Asian Pacific Society of Periodontology Meeting. Asian Pacific Society of Periodontology, 2009, 115-127. |
| Corbet E.F., Ho D.K.L. and Lai S.M.L., Radiographs in periodontal disease diagnosis and management, Australian Dental Journal. 2009, 54 (Suppl 1): S27-S43. |
| Corbet E.F., Reviewer, Community Dental Health. 2009. |
| Corbet E.F., Reviewer, European Journal of Oral Sciences. 2010. |
| Corbet E.F., Reviewer, International Dental Journal. 2009. |
| Corbet E.F., Reviewer, Journal of Dental Research. 2009. |
| Corbet E.F., Reviewer, Journal of Investigative and Clinical Dentistry. 2010. |
| Corbet E.F., Reviewer, Journal of Periodontology. 2009. |
| Corbet E.F., Reviewer, Oral Health and Preventive Dentistry. 2009. |
| Corbet E.F., Reviewer, Quintessence International. 2009. |
| Corbet E.F., Reviewer, Quintessence International. 2010. |
| Tan H., Lo E.C.M., Luo Y., Dyson J.E. and Corbet E.F., Prevention of root caries in Chinese institutionalized elders, Journal of Dental Research. 2009, 88 (Spec Iss C): 129 (WCPD). |
| Researcher : Darveau RP |
| List of Research Outputs |
| Jin L.J., Lu Q., Darveau R.P., Samaranayake L.P. and Wang C.Y., P. gingivalis LPS modulates hBD-2 expression through TLR2 and 4, Journal of Dental Research. 2009, 88 (Spec Iss B): 749 (PAPF/APR). |
| Lu Q., Darveau R.P., Samaranayake L.P., Wang C.Y. and Jin L.J., Differential modulation of human b-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- and penta-acylated lipid A structures, Innate Immunity. 2009, 15: 325-335. |
| Researcher : Darvell BW |
| Project Title: | 40th Annual Scientific Meeting, IADR-ANZ Perth 2000 The Future of Materials Science in Dentistry, the Antidote to Advertising |
| Investigator(s): | Darvell BW |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 07/2000 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Lou Y., Botelho M.G. and Darvell B.W., The Effect of Silver Diammine Fluoride on Demineralized Enamel and Dentine, European Organisation for Caries Research, Budapest, Hungary.. ORCA, 2009, 56th ORCA Congress: Abstract 89. |
| Pan H. and Darvell B.W., Solubility of tetra-calcium phosphate (TTCP) and tricalcium phosphate (β-TCP) by solid titration, Arch Oral Biol. 2009, 54(7): 671-677. |
| Researcher : Deng F |
| List of Research Outputs |
| Siu S.C., Chu F.C.S., Li T.K.L., Chow T.W. and Deng F., Imaging modalities for preoperative assessment in dental implant therapy: an overview, Hong Kong Dental Journal. 2010, 7(1): 23-30. |
| Researcher : Dias APLH |
| Project Title: | 4th Sudanese Dental Conference Rare-earth Alloy Magnets and their Use in Dentistry. |
| Investigator(s): | Dias APLH |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 01/2001 |
| Abstract: |
| N/A |
| Project Title: | Australian Prosthodontic Society Victorian Branch Meeting Dental Magnets and the Magfit Magnetic Retention System |
| Investigator(s): | Dias APLH |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 03/2003 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Researcher : Dyson JE |
| Project Title: | Dental air turbine handpiece bearing failure. |
| Investigator(s): | Dyson JE |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 10/2007 |
| Abstract: |
| Background The air turbine handpiece has been used for most routine dental cutting for over forty years and it remains an essential instrument for much day-to-day clinical dental work. At the time it was introduced in the late 1950s, this instrument was not capable of withstanding heat sterilization due to its corrodible components and, as infection control was not given a high priority at the time, this was accepted by the profession (Dyson and Darvell 1993a, 1993b). However, most air turbine handpieces on the market in recent years have been specified by their manufacturers as being autoclavable although it has been generally accepted that autoclaving results in earlier failure of the handpiece bearings. The need for dental handpieces to be sterilized before each patient use has been the subject of some controversy (Hardie, 1992, 1993). Indeed studies have indicated that many dentists around the world fail to autoclave their handpieces between patients (Treasure and Treasure, 1994, Deskins-Kneble and Rosen, 1993, Lloyd et al., 1995, Dyson et al., 2004). This appears to be related to many dentists’ concern that routine autoclaving will shorten handpiece bearing life (Dyson et al., 2004). In this latter study, 88% of a surveyed group of dentists in Hong believed that autoclaving would shorten handpiece service life. Irrespective of such concerns, it has been recommended (Dyson and Darvell, 1997) that routine autoclaving of all handpieces should be mandatory. A number of studies of the effects of autoclaving air turbine handpiece have been reported based on simulated (Wirthlin, 1981, Angelini, 1992, Worthington et al. 1998, Leonard et al. 1999) and real clinical (Monaghan, 2005) use. Such studies, however, have not revealed the precise mechanisms by which handpiece failure occurs and, furthermore, the relationship of bearing damage to mechanical performance has not been revealed. One recent study (Dyson et al., 2004) involving scanning electron microscopy (SEM) has allowed a system to be developed to categorize deterioration and failure of air turbine handpiece bearings. With further development this would provide a useful tool for studying deterioration of dental bearings and would lead to an improved understanding of their failure. Aims The aims of the proposed work are to develop: • a better understanding of the modes of failure of air turbine handpiece bearings and their effects on handpiece performance • improved guidelines for handpiece selection, sterilization and clinical use • advice for manufacturers on improved handpiece design. Key Issues The key issues to be addressed include elucidation of the influences on handpiece bearing failure of such variables as: • bearing type/design and construction • autoclaving time and temperature • lubrication (lubricant and methods) • contaminants • corrosion inhibitors • handpiece use/abuse between autoclaving cycles. References Angelini E. Influence of sterilization on the corrosion resistance of high-speed dental handpieces. Quintessence Int 1992, Mar; 23(3):215-22. Deskins-Kneble D, Rosen S. Comparison of handpiece asepsis among Ohio dentists: 1991 & 1992. Focus Ohio Dent 1993, 67(2):8-12. Dyson J.E. and Darvell B.W. The development of the dental high speed air turbine handpiece - Part 1. Aust. Dent. J. 1993(a); 38 (1): 49-58. Dyson J.E. and Darvell B.W. The development of the dental high speed air turbine handpiece - Part 2. Aust. Dent. J. 1993(b); 38 (2): 131-144. Dyson J.E. and Darvell B.W. A laboratory evaluation of two brands of disposable air turbine handpiece. Brti. Dent. J. 1997; 182 (1): 15-21. Dyson JE Chau SF and Darvell BW. Air turbine handpiece bearing failure in clinical practice. J Dent Res 2004, 83, Special Issue B, (www.dentalresearch.org) Hardie J. Infectious or not? The handpiece controversy. J Can Dent Assoc 1992, 58 (4) : 281-282. Hardie J. Handpiece sterilization - The debate continues. J Can Dent Assoc 1993, 59 : 355-362. Leonard DL, Charlton DG. Performance of high-speed dental handpieces subjected to simulated clinical use and sterilization. J Am Dent Assoc 1999 Sep 130 (9):1301-11. Lloyd L, Burke FJ, Cheung SW. Handpiece asepsis: a survey of the attitudes of dental practitioners. Br Dent J 1995, 178(1):23-27. Monaghan DM, Wilson NHF, Darvell BW. The Performance of Air-turbine Handpieces in General Dental Practice. Oper Dent 2005, 30-1, 16-25 Treasure P and Treasure ET. Survey of infection control procedures in New Zealand dental practices. Int Dent J 1994, 44(4):342-348. Wirthlin MR Jr, Shklair IL, The performance of autoclaved high-speed dental handpieces. J Am Dent Assoc 1981 Oct;103(4):584-7. Worthington L & Martin MV An investigation of the effect of repeated autoclaving on the speed of some turbines in general dental practice. J Dent 1998, 26(1) 75-77. |
| List of Research Outputs |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience - Journal Based Learning and Fundamentals of Dental Research – a pilot programme, the 6th International Conference on PBL in Dentistry. Hong Kong, 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience-journal based learning and fundamentals of dental research-a pilot programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.26. |
| Bridges S.M., Botelho M.G., Dyson J.E., McGrath C.P.J., Tsang P.C.S. and Yiu C.K.Y., Blended learning and interactive pedagogies: Supporting problem-based learning in dental education, CITE Research Symposium 2010. 2010. |
| Bridges S.M., Corbet E.F., Samaranayake L.P., Dyson J.E., Leung W.K. and McGrath C.P.J., Improving programme evaluations: The effect of curriculum review, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 37. |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Tan H., Lo E.C.M., Luo Y., Dyson J.E. and Corbet E.F., Prevention of root caries in Chinese institutionalized elders, Journal of Dental Research. 2009, 88 (Spec Iss C): 129 (WCPD). |
| Researcher : Fang DTS |
| List of Research Outputs |
| Lo J., Fang D.T.S. and Cheung L.K., Long term Clinical Analysis of Immediate Implant in Aesthetic Zone, Annual Meeting of Philippine Academy of Implant Dentistry. 2009. |
| Researcher : Gao X |
| Project Title: | Validation of caries risk assessment programs in Hong Kong children |
| Investigator(s): | Gao X, Lo ECM, Chu CH |
| Department: | Dental Faculty |
| Source(s) of Funding: | Health and Health Services Research Fund - Full Grants |
| Start Date: | 10/2009 |
| Abstract: |
| To evaluate and compare the performance of various CRA programs (NUS-CRA, CAT, and Cariogram) in Hong Kong preschoolers. |
| Project Title: | Refining a Biopsychosocial Caries Risk Assessment Program with a Chairside Test of Salivary mutans Streptococci |
| Investigator(s): | Gao X, Lo ECM, Chu CH |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2009 |
| Abstract: |
| The goal of this study is to refine a biopsychosocial caries risk assessment program by incorporating an innovative immunoassay product Saliva-Check® mutans. This refined program could potentially serve as a clinically and epidemiologically practical tool for evidence-based treatment planning and cost-effective control of dental caries (the most common chronic childhood disease). The specific aims include: 1. To assess the sensitivity and specificity of a culture-based product (Dentocult® SM Strip mutans) and an immunoassay product (Saliva-Check® mutans), at a single-test basis, in caries prediction among preschoolers. 2. To compare the performance (sensitivity, specificity etc) of the original biopsychosocial program (with Dentocult® SM Strip mutans) and the modified biopsychosocial program (with Saliva-Check® mutans) for predicting caries in preschoolers. |
| List of Research Outputs |
| Gao X., Hsu C.Y.S., Xu Y.C., Loh T., Koh D. and Hwarng H.B., Behavioral Pathways Explaining Oral Health Disparity in Children, Journal of Dental Research. U.S., International Association for Dental Research, 2010, 89(9): 985-90. |
| Gao X., Hsu C.Y.S., Xu Y., Loh T., Koh D. and Hwarng H.B., Behavioral pathways of oral health disparity in children, 2nd Meeting of International Association for Dental Research Pan Asian Pacific Federation and the 1st Meeting of International Association for Dental Research Asia/Pacific Region. U.S., International Association for Dental Research, 2009, . |
| Gao X., Hsu C.Y., Xu Y., Hwarng H.B., Loh T. and Koh D., Building Caries Risk Assessment Models for Children, Journal of Dental Research. 2010, 89(6): 637-643. |
| Gao X., Certificate of Merit, International Association for Dental Research (IADR) South East Asia Division. 2009. |
| Gao X., Seneviratne C.J., Lo E.C.M., Chu C.H. and Samaranayake L.P., Novel and conventional assays for assessing risk of dental caries, In: Programme book of Health Research Symposium 2010: Improving Health and Recognising Excellence. 2010, Ab56: p75. |
| Gao X., McGrath C.P.J. and Lin H.C., Oral Health Status of Rural-Urban Migrant Children in South China, International Dental Association for Dental Research 88th General Session. 2010. |
| Lo E.C.M. and Gao X., Caries risk assessment - practical procedures beyond clinical impression, Oral Health and Science Seminar, Faculty of Dentistry, University of Hong Kong . 2010. |
| Lo E.C.M., Gao X., Lui K.C., Chan B.K.L., Chan Y.H., Cheung S.S., Hung Y.W., Lau K.H.Y., Man W.H.C. and Wu K.W., Oral health status of asthmatic preschoolers in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 167: 1-37. |
| Lo E.C.M., Gao X., Lui K.C., Chan B.K.L., Chan Y.H., Cheung S.S., Hung Y.W., Lau K.H.Y., Man W.H.C. and Wu K.W., Oral-B Prize in Community Oral Health, University of Hong Kong. 2010. |
| Researcher : Hagg EUO |
| List of Research Outputs |
| Bootvong K., Liu Z., McGrath C.P.J., Hagg E.U.O., Wong R.W.K., Bendeus S.A.M. and Yeung S., Virtual model analysis as an alternative approach to plaster model analysis: reliability and validity, The European Journal of Orthodontics. 2010, 32: 589-595. |
| Chau C.M., Wong R.W.K. and Hagg E.U.O., 3-Dimensional Cone-Beam Computerized Tomographic Cephalometric Database on Jaw Dimensions in Chinese, The Open Anthropology Journal 2010. 2010, 3: 2-7. |
| Chau C.M., Hagg E.U.O. and Wong R.W.K., Oral Presentations: Technical Concerns of 3D Cephalometric Measurement Using CBCT Images, The XIIth European Congress of Dento-Maxillo Facial Radiology, Istanbul Turkiye, 2 – 5 June, 2010. |
| Chen Q.G., Zeng Y.S., Qu Z.Q., Tang J.Y., Qin Y.J., Chung P., Wong R.W.K. and Hagg E.U.O., The effects of Rhodiola rosea extract on 5-HT level, cell proliferation and quantity of neurons at cerebral hippocampus of depressive rats, Phytomedicine 2009. 2009, 16: 830-838. |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Antimicrobial activity of Prunes extract on Streptococcus mutans, Second Prize of E-poster Competition for Postgraduate Students. Awarded by IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. 2010. |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Poster Presentation: Antimicrobial activity of Prunes extract on Streptococcus mutans, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Hagg E.U.O., Khanoengnit B., Liu Z., McGrath C.P.J. and Wong R.W.K., Virtual models as an alternative approach to plaster model assessment of tooth dimension, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p106 (SP284). |
| Hibino K., Wong R.W.K., Hagg E.U.O. and Samaranayake L.P., The effects of orthodontic appliances on Candida in the human mouth, The International Journal of Paediatric Dentistry . 2009, 19: 301-308. |
| Hibino K., Samaranayake L.P., Hagg E.U.O., Wong R.W.K. and Lee W., The role of salivary factors in persistent oral carriage of Candida in orthodontic patients, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p151 (SP475). |
| Ikegami T., Wong R.W.K., Lee W., Hibino K. and Hagg E.U.O., The Hybrid Orthodontic Treatment System (HOTS), World Journal of Orthodontics. 2010, 11: 168-179. |
| Mok W., Wong R.W.K. and Hagg E.U.O., Orthodontic treatment planning through spreadsheet programme, Dental Asia July/August 2009. 2009, 43-46. |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia . 2010, March/April 2010: 21-27. |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia November/December 2009. 2009, 24-29. |
| Seneviratne C.J., Samaranayake L.P., Wong R.W.K. and Hagg E.U.O., Antimicrobial activity of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study.Int J Oral Maxillofac Surg. 2010 Apr 23, 2010. |
| Tang A.T.H. and Hagg E.U.O., Composite occlusal bite blocks bonded without liquid resin, 86th Congress of the European Orthodontic Society. 2010. |
| Tang A.T.H. and Hagg E.U.O., Lingual retainers bonded without liquid resin - a 5-year clinical study, The Second International Symposium on Surface and Interface of Biomaterials. 2010. |
| Tang A.T.H. and Hagg E.U.O., Survival analysis of occlusal bite blocks bonded without liquid resin, Annual Conference of International Association of Dental Research, Southeast Asian Division. 2009. |
| Verayannont P., Hagg E.U.O., Wong R.W.K., McGrath C.P.J. and Yeung S., Maxillary growth during puberty determined by the implant method compared with cephalometry, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p70 (SP119). |
| Wey M.C., Wu C.L., Wong R.W.K., Zamri R. and Hagg E.U.O., Posterior Teeth Mesialization With Mini-implants In An Oligodontia Patient, Malaysian Dental Journal. 2009, 29(2): 113-118. |
| Wiechmann D., Wong R.W.K. and Hagg E.U.O., Orthodontic Treatment using Incognito; new lingual orthodontic appliance applicating CAD/CAM, Journal of Orthodontic Practice (Japanese version). 2009, 83-90. |
| Wong R.W.K., Hagg E.U.O., Samaranayake L.P., Yuen M.K.Z., Seneviratne C.J. and Kao R., Antimicrobial activities of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study, International Journal of Oral & Maxillofacial Surgery. 2010, 39: 599-605. |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath C.P.J., Comprehensive Cephalometric Analyses of 10 to 14-Year Old Southern Chinese, The Open Anthropology Journal 2010. 2010, 3: 85-95. |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath M., Poster Presentations: Sagittal Occlusal Analysis of 12-year old Southern Chinese Children, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Yuen K., Wong R.W.K., Hagg E.U.O. and Samaranayake L.P., Antimicrobial activities of forty traditional Chinese medicines on oral bacteria: A experimental study, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p152 (SP480). |
| Researcher : Hiraishi N |
| List of Research Outputs |
| Hiraishi N., Yiu C.K.Y., King N.M., Tagami J. and Tay F.R.C.M., Antimicrobial efficacy of 3.8% silver diamine fluoride and its effect on root dentin, Journal of Endodontics. 2010, 36(6): 1026-1029. |
| Researcher : Ho KW |
| List of Research Outputs |
| Chee C., Ho K.W., Yau S.W., Chu C.H. and Lo E.C.M., Oral hygiene and gingival status of Hong Kong pre-school children, Journal of Dental Research. 2009, 88 (Spec Iss B): 656 (PAPF/APR). |
| Researcher : Ho PL |
| List of Research Outputs |
| Ho P.L., Liu H.N., Lam C., Chu C.H. and Lo E.C.M., Occlusal featurs of primary dentition in Chinese preschool children, Journal of Dental Research. 2009, 88 (Spec Iss B): 601 (PAPF/APR). |
| Researcher : Itthagarun A |
| Project Title: | New concepts for selective removal of carious dentition |
| Investigator(s): | Itthagarun A, King NM, Tay FRCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To evaluate and compare the bonding efficacy of cavities prepared by 'SmartPrep' & 'CalrisolvTM on primary teeth, with those prepared by a conventional bur; to evaluate and compare the bonding efficacy of cavities prepared by 'CalrisolvTM, on primary teeth, with those prepared by a conventional bur; to evaluate and compare the bonding efficacy of cavities prepared, in primary teeth, by 'SmartPrep' with those prepared by CarisolvTM. |
| Project Title: | The effect of R.O.C.S (remineralizing oral care system) toothpaste on artificial caries like lesions: An in vitro pH cycling study. |
| Investigator(s): | Itthagarun A, King NM, Rana R |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 10/2005 |
| Abstract: |
| Recent advances in the technique of processing microradiographs by computer-aided image analysis allow the mineral profiles of enamel lesions to be assessed with great accuracy (Strang et al., 1987; Heaven et al., 1990; Chow et al., 1992). It is now possible to measure small changes in these profiles following exposure of the lesions to demineralization and/or remineralization in laboratory models, as well as in the oral environment (Strang et al., 1986). Unfortunately, the comparison of experimental and control sections is hampered by the biological variation in the enamel itself, especially by inhomogeneous lesions, even when they are prepared under standardized conditions (Strang et al., 1986). Several research groups have developed a 'single section technique', mainly for the purpose of determining differences between pre- and post experimental profiles at exactly the same spot (Creanor et al., 1986; Mellberg et al., 1986; Wefel et al., 1987). Since thin sections can be repeatedly subjected to microradiographic assessment during or between experimental treatments, this technique offers the possibility of determining time-dependent changes in lesion profiles resulting from localized dissolution or deposition of enamel. It may thus help to resolve the mechanisms which underlie the formation of subsurface lesions and laminations (Driessens et al., 1986; Palamara et al., 1986; Sato and Yamamoto, 1986), and the effects of both caries-protective agents and inhibitors of remineralization. Ten Cate and Duijsters (1982) developed a so-called 'pH cycling model' involving tooth enamel and intra-oral-like conditions of de/remineralization solution, and pH ranges. This in vitro technique has been widely accepted because it can simulate in vivo situations more closely than traditional experimental studies. Since then, many researchers have adopted and modified such pH cycling methods to use in both enamel and root lesion experiments (Featherstone, 1983; Ten Cate et al., 1988; Heilman et al., 1991; Itthagarun et al., 1997). The version developed by Itthagarun et al., has been used extensively to evaluate remineralizing agents in toothpaste, chewing gums and lozenges (Itthagarun et al., 1999; 2000; 2004; 2005). Recently R.O.C.S toothpastes (Remineralizing oral care systems, DRC Ltd, Moscow, Russia) have been marketed for school children and adults. It has been claimed by the company that these toothpastes 'accelerate the formation of enamel' and 'primary caries focus is enriched with minerals'. The main active ingredients of R.O.C.S. for school-children are complex AMIFLUOR® (minerals) with fluorine and xylitol, while the R.O.C.S toothpaste intend for use by adults contains MINERALIN® and bromelaine, a source of calcium, phosphorus and magnesium. Currently, no published data are available in the English literature. Xylitol has been found to induce remineralization of demineralized enamel by facilitating calcium movement and accessibility (Miake et al, 2003). It also raises the pH of saliva and changes both the quantity and quality of saliva, thus aiding the remineralization process. Bromelaine has been found to provide an anti-inflammatory effect and has been used in medicine and in dentistry, following third molar extractions (Hotz et al, 1989) Consequently, the essence of the remineralizing concept of R.O.C.S. might be achieved by simultaneously supplying calcium, phosphate, and fluoride ions to the teeth in order to induce the formation of calcium fluoroapatite which remineralizes and strengthens the tooth (Torrado et al, 2004). However, as no evidence on the remineralization effects of R.OC.S toothpaste has been published in the English literature, it is appropriate to evaluate the remineralizing effects claimed and also to determine the mechanics involved. Objectives of the study: The aims of this in vitro pH cycling study is to use the single-section technique together with our modified pH cycling model to evaluate the effects of R.O.C.S schooldchildren and R.O.C.S adult toothpastes on the remineralization of enamel artificial caries-like lesions. Their remineralization efficacy will also be compared with that of a standard fluoride toothpastes intended for both children and adults. Key issues and problems to be addressed: Hypothesis to be tested o The new R.O.C.S. toothpastes for children will enhance remineralization of initial carious lesions in tooth enamel more effectively than non-fluoride child toothpaste o The new R.O.C.S. toothpastes for children will show similar 'healing' efficacy of the enamel initial cariuos lesions when compared to that of a standard fluoride toothpaste for children (500ppmF) o The new R.O.C.S. toothpastes for adults will enhance remineralization of initial carious lesion in tooth enamel more effectively than non-fluoride toothpaste o The new R.O.C.S. toothpastes for adults will show similar 'healing' efficacy of the enamel initial carious lesions when compared to that of the standard fluoride toothpaste for adults (1000ppmF) |
| List of Research Outputs |
| Lo E.C.M., Zhi Q. and Itthagarun A., Comparing two quantitative methods for studying remineralization of artificial caries, Journal of Dentistry. 2010, 38: 352-359. |
| Researcher : Jayaratne YSN |
| List of Research Outputs |
| Jayaratne Y.S.N., Lo J., Zwahlen R.A. and Cheung L.K., 3-D Photogrammetry for Surgical Planning of Tissue Expansion in Hemifacial Microsomia., Head Neck. PMID 19862829, 2009. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Computer Aided Maxillofacial Surgery: An Update (accepted), Surgical Innovations. 2010. |
| Jayaratne Y.S.N., Fulbright fellowship at the Harvard Medical School, USA. (This visit will be funded by RGC-Fulbright Hong Kong Dissertation Research Program and augmented with the stipend provided by the University of Hong Kong for postgraduate students) July 2010 - March 2011. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A. and Cheung L.K., Three-dimensional anthropometric analysis of the Chinese nose, J Plast Reconstr Aesthet Surg. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Three-dimensional color maps: a novel tool for assessing craniofacial changes., Surg Innov. 2010. |
| Researcher : Jayaratne YSN |
| List of Research Outputs |
| Jayaratne Y.S.N., Lo J., Zwahlen R.A. and Cheung L.K., 3-D Photogrammetry for Surgical Planning of Tissue Expansion in Hemifacial Microsomia., Head Neck. PMID 19862829, 2009. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Computer Aided Maxillofacial Surgery: An Update (accepted), Surgical Innovations. 2010. |
| Jayaratne Y.S.N., Fulbright fellowship at the Harvard Medical School, USA. (This visit will be funded by RGC-Fulbright Hong Kong Dissertation Research Program and augmented with the stipend provided by the University of Hong Kong for postgraduate students) July 2010 - March 2011. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A. and Cheung L.K., Three-dimensional anthropometric analysis of the Chinese nose, J Plast Reconstr Aesthet Surg. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Three-dimensional color maps: a novel tool for assessing craniofacial changes., Surg Innov. 2010. |
| Researcher : Jayatilake JAMS |
| List of Research Outputs |
| Samaranayake Y.H., Yau J.Y.Y., THEIN Z.M., Jayatilake J.A.M.S., Yeung S.K.W. and Samaranayake L.P., The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its imact on the colonization traits of Candida glabrata, Medical Mycology. 2010, 1-10. |
| Researcher : Jin LJ |
| Project Title: | Tongue cleaning versus mouthrinsing in periodontal patients –their effect on halitosis and quality of life before and after non-surgical therapy |
| Investigator(s): | Jin LJ, McGrath CPJ, Corbet EF |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2007 |
| Completion Date: | 09/2009 |
| Abstract: |
| Tongue cleaning versus mouthrinsing on halitosis Halitosis (bad breath or oral malodor) which describes any disagreeable odor of expired air regardless of origin is a frequent complaint by many dental patients (Tangerman 2002). Its etiology can be exogenous (e.g. food, smoking and alcohol), endogenous (e.g. oral, drug-induced or systemic diseases) or psychogenic (e.g. pseudohalitosis and halitophobia). When amino acids are degraded by anaerobic and predominantly gram-negative bacteria, unpleasant smelling volatile sulphur compounds (VSC) including hydrogen sulfide, methyl mercaptan, dimethyl sulfide are produced (Tonzetich 1964, Rosenberg 1996). About 90% of halitosis cases originate within the oral cavity (Tonzetich 1977), mostly associated with periodontal diseases and/or tongue coating (van Steenberghe 2004). Patients with periodontal diseases tend to exhibit a higher prevalence of the problem compared to healthy individuals (Yaegaki 1992), likely due to higher levels of anaerobic, gram-negative bacteria residing in periodontal pockets (Morita 2001), increased gingival bleeding tendency which may provide essential substrates for odor production (Quriynen 1998) as well as increased flow of gingival crevicular fluid providing more methionine (Yaegaki 1992). Various methods have been used to assess the levels of malodor. Organoleptic measurement with the human nose has been claimed the most reliable and practical way (Rosenberg 1996). However, the use of a halimeter or sulphide monitor gives more reproducible results (Silwood 2001). Other methods include culture of plaque and periodontal pocket exudates (Loesche 1995) and gas chromatography coupled with flame-photometric detection (Solis-Gaffar 1975). At present, there is no consensus on a clinical protocol for the management of halitosis (Pratibha 2006). The tongue forms the largest oral niche for microorganism colonization in the oral cavity and acts as a reservoir for the accumulation of bacteria and food residues (Scully 1997). Tongue cleaning has been shown to effectively reduce halitosis (Suarez et al 2000, Danser 2003) by preventing putrefaction and removing the substratum for VSC-producing bacteria (Quirynen 2004). However, it may cause discomfort, nausea, gagging and trauma to the tongue mucosa (Ito et. al. 2000), if it is performed inappropriately. The use of a toothbrush for tongue cleaning is convenient for most people because it does not require an additional tool, but tongue scrapers have been shown to give a significantly more reduction in VSCs than toothbrush (Pedrazzi et. al. 2004). Mouthrinsing is a socially acceptable way to alleviate or mask halitosis (Wennstrom 1998, Borden 2002). Chlorhexidine (CHX) has been “the leading oral antiseptic” for the last two decades due to its anti-plaque effect. Its oral use does not give rise to systemic toxicity, microbial resistance or suprainfection (Strydonck et al 2005). Numerous studies have compared different concentrations of CHX with other mouthwashes for their anti-halitosis effect, including triclosan, essential oils, cetylpyridinium chloride, and zinc acetate (Rosing 2002, Carvalho 2004). All gave a reduction in VSC level when compared to negative controls. However, 0.2% CHX (Corsodyl® ) gives superior results because of its better substantivity and it is therefore accepted as the gold standard (Kozlovesky et. al. 1996, van Steenberghe et. al. 2001, Young et. al. 2003). Its side effects from long-term use include tooth discoloration, taste perturbation and mucosal irritation. CHX efficacy is more dependent on dose than its concentration (Bonesvoll and Germo 1978, Keijser et al 2003). The optimal dose of CHX is generally considered to be in the region of 20mg (e.g. 10mL 0.2% CHX or 15mL 0.12% CHX) twice daily, rinsing for 60s each time (Cummings and Loe 1973, Agerbaek et al 1975, Jenkins et al 1994), which balances efficacy against local side effects and user acceptability (Flotra et. Al. 1971, Van Strydonck et. al. 2005). A combination of tongue cleaning and mouthrinsing helps to reduce halitosis significantly (Roldan et al 2005). Currently, there has not been any randomized, controlled trial directly comparing tongue cleaning against mouthrinsing (Outhouse et. al. 2006). Impact of halitosis on the quality of life of periodontal patients Oral health-related quality of life is defined as an individual’s assessment of how the following affect his or her wellbeing: functional factors, psychological factors, social factors, and experience of pain/discomfort in relation to orofacial concerns (Inglehart & Bagramian 2002). Halitosis can be one of the important factors impacting on quality of life, contributing to social embarrassment, low self-esteem, social isolation and emotional stress (Needleman 2004). Efforts have been made in recent years to understand and embrace patient perceptions of the impact of oral health on their lives. It has been shown that periodontal status impacts on the life quality of periodontal patients, and those patients complaining of halitosis have significantly lower life quality scores than those without the complaint (Needleman et. al. 2004, Ng & Leung 2006). Currently, the Oral Health Impact Profile (OHIP) is one of the most comprehensive subjective oral health status measures consists of 49 items divided into seven domains (Locker 1988), and it has been used in numerous clinical trials relevant to studies on quality of life (Allen & McMillan 1999, McGrath et. al. 2003, John et. al. 2004). Tongue cleaning versus mouthrinsing on periodontal treatment outcomes Results showed that one-stage full mouth disinfection (Koshy 2004) gives superior outcomes than other forms in both periodontal parameters and halitosis reduction (Quirynen 1998, 2006). Previous studies regarding tongue cleaning have focused on periodontitis-free patients, or those with treated periodontitis (Roldan 2005, Faveri 2006). Few have looked into its effect on patients undergoing active periodontal therapy. In addition, no research has yet explored whether tongue cleaning, without the use of CHX rinses or irrigation, can significantly reduce the decolonization of debrided periodontal pockets. If this is the case, then root debridement supplemented with tongue cleaning may give effective clinical outcomes without the use of CHX which can cause the aforementioned side effects. The objectives of the present study are i) to compare the effect of tongue cleaning and mouthrinsing on halitosis in periodontal patients; ii) to assess the impact of halitosis reduction on the quality of life; and iii) to compare the change in periodontal parameters following non-surgical therapy between the control, tongue cleaning and mouthrinsing groups. |
| Project Title: | Effects of a diode laser in modulating inflammation in Chinese adults with chronic periodontitis - A randomised split-mouth design study |
| Investigator(s): | Jin LJ, Corbet EF |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 10/2008 |
| Abstract: |
| Laser is an acronym for Light Amplification by Stimulated Emission of Radiation, and this has been described by the European Standard IEC 601 as “any device which can be made to produce or amplify electromagnetic radiation in the wavelength range from 180nm to 1mm primarily by the process of controlled stimulated emission.” The first laser use of dentistry was revealed in 1989 using the active medium of Nd:YAG emitted pulsed light. Currently, there are several types of dental lasers available, named according to their active medium, wavelength, delivery system, emission mode(s), tissue absorption and clinical applications, including Argon lasers, diode, neodynium:YAG, Holmium:YAG, erbium family and CO2 lasers. The shorter wavelengths are absorbed more readily by soft tissues but not dental hard tissues, whilst the longer wavelengths can be absorbed by water and hydroxyapatite and therefore can be used to cut into dental hard tissue such as bone or enamel. Another classification can be derived from the effect of a particular laser on the target cell, tissue or organism, such as photochemical, photothermal and photoablative or photomechanical effects (Wilson, 1994). Soft lasers such diode lasers are generally used for soft tissue sculpting and bacterial reduction, which induce the photochemical changes. Low power lasers produce less than one Joule at an energy density of only several J/cm2. Low power laser light can only affect the viability or activities of a bacterial cell, if it is absorbed by one or more of its constituents. Once absorption occurs, this promotes the electrons to a higher energy level. When the molecule returns to its ground state, the excess energy is released as heat, light or inducing a chemical reaction. The triplet energy exchange can cause oxygen to undergo an electronic transition to its singlet state, which can cause microbial cell death by interfering with metabolic activities or by disrupting certain structures like lipid peroxidation and protein agglutination. Multiple uses of lasers in periodontal treatment have been tested, such as CO2 and Nd:YAG lasers (Cobb et al., 1992; Pick and Colvard, 1993; Gold and Vilardi, 1994; White et al., 1998; Eberhard et al., 2003; Schwarz et al., 2003). The protohaemin and protoporphyrin black-pigmented bacteria seem to be most susceptible to short wavelength lasers, including the Porphyromonas and Prevotella species (Wilson, 1994). The development of diode cells to produce laser light allows these machines to be much smaller and less bulky than others (Parker, 2007). The active medium in diode laser includes Indium-Gallium-Arsenide-Phosphide, Gallium-Aluminium-Arsenide or Gallium-arsenide sandwiched between silicone wafers. The wavelengths are in the 655, 810 or 980 nm ranges. Diode lasers have been compared to Nd:YAG lasers, as both emit radiation within the infrared range at a very similar wavelength (Moritz et al., 1998). However whilst the Nd:YAG laser can create a marked temperature rise in the deeper tissues, the diode laser is absorbed by the superficial periodontal tissues. Diode lasers have been increasingly used in dentistry. It could be used safely in non-surgical periodontal treatment (Castro et al., 2006). Some previous studies showed that there are measurable changes in periodontal status when a diode laser is introduced in combination with routine periodontal treatment. (Moritz et al., 1998; Borrajo et al., 2004; Kreisler et al., 2005; Qadri et al., 2005). It resulted in a significant reduction in bacterial populations and improved the bleeding on probing scores and pocket depths as well as reduced the incidence of bacteraemia, when compared to periodontal treatment alone (Moritz et al., 1998; (Kimura et al., 2000; Mohammad et al., 2007). Diode laser irradiation has shown in vitro to stimulate proliferation in periodontal ligament cells (Kreisler et al., 2003). Recently, it is also suggested that the diode laser’s ability to de-epithelize the periodontal pocket can lead to improved connective tissue attachment and therefore a reduction in tooth mobility and probing depths (Kreisler et al., 2005). Currently, there is a lack of clinical studies examining the use of diode lasers in periodontal treatment and an inconsistency regarding the wavelength and power and whether continuous or pulsation beam modes could provide the appropriate treatment benefit. Gingival crevicular fluid (GCF) is an inflammatory exudate which originates from periodontal tissues. Its components come primarily from the leakage of postcapillary venules, with additional input from the intercellular fluid and cellular cytoplasm. The constituents of GCF are derived from multiple sources including serum, connective tissue and epithelium, inflammatory cells and bacteria. The collection of GCF has shown to be a non-invasive method of detecting inflammatory markers for periodontal disease progression (Offenbacher et al., 1993). The collection of GCF using pre-cut filter strips have shown to be reproducible and reliable, and a quantitative means of evaluating the effectiveness of periodontal treatment (Lamster, 1997; Jin and Darveau 2001; Jin et al. 2003). Several host response mediators in GCF have been proposed as potential diagnostic tests for periodontal disease, such as prostaglandin E2 (Offenbacher et al., 1981), granulocyte elastase (Jin et al. 1999), IL-1ra (Rawlinson et al., 2000) and IL-1β (Zhong et al., 2007). There are currently few studies exploring the adjunctive in-vivo effects of diode laser in modulating gingival inflammatory response. This study aims to investigate the effects of diode laser as an adjunct of non-surgical periodontal treatment on early inflammatory response of periodontal tissues. In addition to clinical parameters, inflammatory markers in GCF will be measured as a mean of assessing local expressions of periodontal inflammation and healing response. Aim To investigate the effects of diode laser therapy as an adjunct to non-surgical periodontal treatment on modulating inflammation in subjects with moderate to advanced periodontitis. Objectives -To evaluate the effects of diode laser treatment on the inflammatory components in GCF in subjects with moderate to advanced periodontitis. -To evaluate the clinical effects of diode laser treatment in subjects with moderate to advanced periodontitis. |
| Project Title: | A clinical investigation on the association of periodontal disease with endothelial biomarkers and dysfunction |
| Investigator(s): | Jin LJ, Tse HF, Li X |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2009 |
| Abstract: |
| Introduction Periodontal diseases, including gingivitis and periodontitis, are among the most commonly occurring infections in humans. They are initiated by pathogenic plaque biofilms and characterized by bacteria-induced inflammatory destruction of tooth-supporting structures and alveolar bone. Currently, periodontitis remains the most common cause of tooth loss in adults. Severe periodontal disease affects 5–20% of adult populations worldwide (Petersen et al. 2005). About 72% of adults in Hong Kong aged 35-44 years have mild forms of periodontal disease, and approximately 17% may experience more advanced forms of the disease (Holmgren et al. 1994). In periodontitis, the highly inflamed periodontal pocket epithelia are easily broached. The impacts of uncontrolled periodontal infections on systemic health are apparent and they may therefore serve as a reservoir for spillover of bacteria, bacterial products and inflammatory cytokines/mediators (Page et al. 1997, Jin et al. 2003) into the circulation with resultant various levels of bacteremias, endotoxemias and cytokinemias, subsequently affecting the vascular endothelium and other cells and tissues distant from periodontal tissues (Thoden et al. 1984, Jin 2008, Parahitiyawa et al. 2009). Key issues and problems addressed Emerging evidence suggests that periodontal disease may be associated with cardiovascular disease (CVD) by contributing to an increased level of systemic inflammation (Jin 2008). Periodontitis is associated with a cluster of systemic biomarkers of inflammation including C-reactive protein (CRP) (D'Aiuto et al. 2004, Joshipura et al. 2004, Buhlin et al. 2009) and Interleukin-6 (IL-6) (D'Aiuto et al. 2004), high- and low-density lipoprotein cholesterols (Katz et al. 2001), von Willebrand factor, tissue plasminogen activators (Joshipura et al. 2004), and levels of antibodies induced by periodontal pathogens (Pussinen et al. 2003). Endothelial dysfunction has been recognized as an early marker of preclinical CVD (Faulx et al. 2003). Brachial artery flow-mediated dilation (FMD) is a noninvasive measurement of endothelial dysfunction (Faulx et al. 2003). In recent years, circulating endothelial progenitor cells (EPC) have received much attention (Asahara et al. 1997, Hristov et al. 2003). They can express surface antigens of both hematopoietic stem cells and endothelial cells (Hristov et al. 2003), and participate in endothelial repair of vasculature and maintenance of vascular integrity (Asahara et al. 1997). The commonly used markers for EPC identification include CD34, CD133 and kinase-insert-domain-containing receptor (KDR). CD34 and CD133 are antigens of hematopoietic stem cells, and KDR is a marker of endothelial cells (Fadini et al. 2006). CD34-/CD133+/KDR+ reflects less mature EPC and CD34+/CD133-/KDR+ reflects more mature EPC (Wojakowski et al. 2008). The number and function of EPC are related to CVD, and this association may reflect the host capacity to repair the injured vasculature (Kunz et al. 2006). Indeed, the number of EPC is proposed as a surrogate marker for endothelial dysfunction and cumulative cardiovascular risk (Hill et al. 2003), and shown to be correlated with the endothelial function as assessed by FMD (Hill et al. 2003, Wojakowski et al. 2008). Control of periodontal infection could reduce serum levels of certain biomarkers related with atherosclerosis including CRP and IL-6 (Fokkema et al. 2003, D'Aiuto et al. 2004, Pussinen et al. 2004), and significantly improve endothelial function assessed by both FMD (Mercanoglu et al. 2004, Blum et al. 2007, Tonetti et al. 2007) and carotid intima-media thickness (IMT) (Piconi et al. 2009). However, the relevant mechanism remains to be clarified. Currently, no study is available on the association between periodontal disease and EPC. Hypotheses - Periodontal disease is associated with EPC in the blood circulation and endothelial dysfunction. - Periodontal treatment may affect the levels of EPC and improve endothelial dysfunction with the decrease of systemic inflammation. Objectives - To investigate the association of periodontal disease with circulating EPC and endothelial dysfunction in Hong Kong Chinese. - To investigate the effects of periodontal treatment on the levels of EPC and endothelial dysfunction through randomized, controlled clinical trials. References Asahara T, Murohara T, Sullivan A. et al. Science 1997; 275:964-967. Blum A, Kryuger K, Mashiach Eizenberg M. et al. Eur J Intern Med 2007; 18:295-298. Buhlin K, Hultin M, Norderyd O. et al. J Clin Periodontol 2009; 36:541-549. D'Aiuto F, Parkar M, Andreou G. et al. J Dent Res 2004; 83:156-160. Fadini GP, de Kreutzenberg SV, Coracina A. et al. Eur Heart J 2006; 27:2247-2255. Faulx MD, Wright AT, Hoit B. Am Heart J 2003; 145:943-951. Fokkema SJ, Loos BG, Hart AA et al. J Clin Periodontol 2003; 30:756-760. Hill JM, Zalos G, Halcox JP. Et al. N Eng J Med 2003; 348:593-600. Holmgren CJ, Corbet EF, Lim LP. Community Dent Oral Epidemiol. 1994; 22(5 Pt 2):396-402. Hristov M, Erl W, Weber PC. Arterioscler Thromb Vasc Biol 2003; 23:1185-1189. Jin LJ. A perspective of periodontal systemic relationships for the Asian Pacific region. eds. Bartold PM, Ishikawa I, Zhang J.; Adelaide: Asian Pacific Society of Periodontology, 2008; p.114-121. Jin LJ, Chiu GKC, Corbet EF. Hong Kong Med J 2003; 9:31-37. Joshipura KJ, Wand HC, Merchant AT et al. J Dent Res 2004; 83:151-155. Katz J, Chaushu G, Sharabi Y. J Clin Periodontol 20001; 28:865-868. Kunz GA, Liang G, Cuculi F. et al. Am Heart J 2006; 152: 190-195. Lau KK, Chan YH, Yiu KH. et al. J Hum Hypertens 2007; 21:445-451. Lee IG, Chae SL, Kim JC. Eye 2006; 20:546-552. Mercanoglu F, Oflaz H, Oz O, et al. J Periodontol 2004; 75: 1694-1700. Page RC, Offenbacher S, Schroeder HE, et al. Periodontol 2000 1997; 14:216-248. Parahitiyawa NB, Jin LJ, Leung WK et al. Clinical Microbiology Reviews 2009; 22:46-64. Petersen PE, Bourgeois D, Ogawa H, et al. Bull World Health Organ 2005; 83:661-669. Petrie A, Bulman JS, Osborn JF. Br Dent J. 2002; 193:557-561. Piconi S, Trabattoni D, Luraghi C, et al. FASEB J 2009; 23:1196-1204. Pussinen PJ, Jauhiainen M, Vilkuna-Rautiainen T, et al. J Lipid Res 2004; 45:139-147. Pussinen PJ, Jousilahti P, Alfthan G, et al. Arterioscler Thromb Vasc Biol. 2003; 23:1250-1254. Thoden van Velzen SK, Abraham-Inpijn L, Moorer WR. J Clin Periodontol 1984; 11:209-220. Tonetti MS, D'Aiuto F, Nibali L, et al. N Eng J Med 2007; 356:911-920. Wojakowski W, Kucia M, Kazmierski M, et al. Heart 2008; 94:27-33. |
| Project Title: | Modulation of innate immune responses in human gingiva by P. gingivalis lipopolysaccharide with lipid A heterogeneity Novel molecular mechanisms and clinical implications |
| Investigator(s): | Jin LJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 11/2009 |
| Abstract: |
| 1) Determine the roles of TLRs in P. gingivalis LPS-induced modulation of global expression profiles of innate immune responses in gingival epithelial cells; 2) Investigate the inhibitory effects of two synthetic LPS antagonists (E5564 and E5531) on P. gingivalis LPS-induced modulation of innate immune responses in gingival epithelial cells; 3) Determine the roles of IKK-NF-{happa}B signaling on P. gingivalis LPS-induced modulation of the expression profiles of hBDs as well as pro- and anti-inflammatory cytokines in gingival epithelial cells. |
| List of Research Outputs |
| Ajonuma L.C., Lu Q., Cheung B.P.K., Leung W.K., Samaranayake L.P. and Jin L.J., Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva, Cell Biology International. 2010, 34: 147-152. |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development, BMC Microbiology. 2010, 10: 125 (pages 1-9). |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation, Journal of Medical Microbiology. 2009, 58: 1623-1631. |
| Jin L.J., Co-author of the Research Output Prize, Faculty of Dentistry, The University of Hong Kong, June 2009: Seneviratne J., Wang Y., Jin L.J. Abiko Y. & Samaranayake L.P. (2008). Candida albicans biofilm formation is associated with increased anti-oxidative capacities, Proteomics 2009; 8:2936-2947. 2009. |
| Jin L.J., Editorial Board Member (2010.1- 2012.12) , Journal of Dental Research . 2010. |
| Jin L.J., Lu Q., Darveau R.P., Samaranayake L.P. and Wang C.Y., P. gingivalis LPS modulates hBD-2 expression through TLR2 and 4, Journal of Dental Research. 2009, 88 (Spec Iss B): 749 (PAPF/APR). |
| Li X., Tse H.F., Yiu K.H., Jia N., Chen H., Li L.S.W. and Jin L.J., Increased levels of circulating endothelial progenitor cells in subjects with moderate to severe chronic periodontitis, Journal of Clinical Periodontology. 2009, 36: 933-939. |
| Li X., Tse H.F., Li L.S.W. and Jin L.J., Salivary MIF is associated with gingival inflammation and periodontopathogens, Journal of Dental Research. 2009, 88 (Spec Iss B): 288 (PAPF/APR). |
| Loo T...Y...W..., Jin L.J., Cheung N.B.M. and Chow L.W.C., Evaluation of Ellagic acid on the activities of oral bacteria with the use of adenosine triphosphate (ATP) bioluminescence assay, African Journal of Biotechnology. 2010, 9: 3938-3943. |
| Loo T.Y., Jin L.J., Cheung M.N.B. and Dou Y.D., Detection of Bacteroides forsythus and Porphyromonas gingivalis in infected root canals during periapical periodontitis by 16S rDNA, African Journal of Biotechnology. 2009, 8: 2021-2026. |
| Loo T.Y., Jin L.J., Cheung M.N.B. and Wang M., The impact of diabetes on the success of dental implants and periodontal healing, African Journal of Biotechnology. 2009, 8: 5122-5127. |
| Loo T.Y., Jin L.J., Cheung M.N.B., Dou Y.D. and Chen J.P., Polistes olivaceous decreases biotic surface colonization, African Journal of Biotechnology. 2009, 8: 7094-7100. |
| Loo T.Y.W., Jin L.J., Cheung N.B.M., Chow L.W.C. and Wang M., Combination of radiological and biochemical methods to assess bone mineral density of mandible in fully edentulous patients after chemotherapy: a 5-year prospective study, Expert Opinion on Investigational Drugs. 2010, 19 (Suppl 1): S109-S115. |
| Loo T.Y.W., Jin L.J., Chow L.W.C., Cheung M.N.B. and Wang M., Rhodiola algida improves chemotherapy-induced oral mucositis in breast cancer patients, Expert Opinion on Investigational Drugs. 2010, 19 (Suppl 1): S91-S100. |
| Lu Q., Darveau R.P., Samaranayake L.P., Wang C.Y. and Jin L.J., Differential modulation of human b-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- and penta-acylated lipid A structures, Innate Immunity. 2009, 15: 325-335. |
| Parahitiyawa N.B., Scully C., Leung W.K., Yam W.C., Jin L.J. and Samaranayake L.P., Exploring the oral bacterial flora: current status and future directions, Oral Diseases. 2010, 16: 136-145. |
| Pienvichit P., Tongson L.S. and Jin L.J., Periodontal disease and systemic diseases: An inter-link, Medical Tribune. 2009, January: Hong Kong Focus - IV. |
| Ren L., Fu Y., Deng Y., Qi L. and Jin L.J., Advanced glycation end products inhibit the expression of collagens type I and III by human gingival fibroblasts, Journal of Periodontology. 2009, 80: 1166-1173. |
| Ren L., Jiang Z.Q., Fu Y., Leung W.K. and Jin L.J., The interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease, Journal of Clinical Periodontology. 2009, 36: 619-626. |
| Seneviratne C.J., Silva W.J., Jin L.J., Samaranayake Y.H. and Samaranayake L.P., Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms, Archives of Oral Biology. 2009, 54: 1052-1060. |
| Seneviratne C.J., Wang Y., Jin L.J., Abiko Y. and Samaranayake L.P., Proteomics of drug resistance in Candida glabrata biofilms, Proteomics 2010 Apr;10(7):1444-54., 2010. |
| Seneviratne C.J., Wang Y., Jin L.J., Abiko Y., Watamoto T. and Samaranayake L.P., Shotgun proteomics elucidates the regulatory pathway of Candida biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 254 (PAPF/APR). |
| Researcher : Ke J |
| List of Research Outputs |
| Ke J., Cheung L.K. and Zheng L., The best poster award at the 3rd Hiroshima Conference, Hiroshima University. 2009. |
| Researcher : King NM |
| Project Title: | Aesthetic concerns of adolescents and young adults in relation to development defects of enamel |
| Investigator(s): | King NM, Lo ECM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2002 |
| Abstract: |
| To develop a set of standardized photographic images showing permanent anterior teeth with developmental enamel defects of different types suitable for investigating people's perception of the aesthetics; to determine the aesthetic perception and the need for treatment of adolescents and young adults in relation of different enamel defects in Chinese (Hong Kong); to evaluate the effect of gender, age, dental training (knowledge) and dental impairment on people's aesthetic perception of developmental enamel defects. |
| Project Title: | Remineralization ability of hydroxyapatite containing dentifrices |
| Investigator(s): | King NM, Itthagarun A, Ng YW |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To explore an in vitro pH cycling study of the progression of initial enamel caries lesion treated with commercially available dentifrices contain hydroxyapatite crystals. |
| Project Title: | The effects of different concentrations of water on the bonding efficacy to dentin of self-etching primers |
| Investigator(s): | King NM, Tay FRCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To create experimental self-etching primers using one acidic resin monomer, one hydrophilic resin monomer, a fixed amount of ethanol and variable amounts of water for simultaneous etching and priming of dentin. |
| Project Title: | Microtensile bond testing of luting cements to dentin |
| Investigator(s): | King NM, Yiu CKY, Hiraishi N |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 07/2007 |
| Completion Date: | 12/2009 |
| Abstract: |
| Objectives: (1) To compare the microtensile bond strength of two self-etch resin luting cements to dentin prepared with either a coarse or superfine diamond bur. (2) To evaluate the effect of simulated pulpal pressure on permeability and microtensile bond strength of two self-etch resin luting cements to dentin. (3) To evalauate the effect of seating pressure on the microtensile bond strength of two self-etch resin luting cements to perfused dentin. The null hypothesis: (1) There is no difference in the microtensile bond strength of self-etch resin luting cements to dentin prepared with different surface treatments. (2) The increase in adhesive permeability induced by simulated pulpal pressure has no effect on microtensile bond strength of self-etch resin luting cements to dentin. (3) Seating pressure has no effect on the microtensile bond strength of self-etch resin luting cements to perfused dentin. Key issues and problems: 1. Tooth-colored resin composites have become the primary aesthetic restorative material to replace tooth structure lost through dental caries and trauma. Indirect composite restorations are preferred over direct restorations because of their improved mechanical properties and reduced polymerization contraction stress. A key element for the success of these indirect bonded restorations is the intimate interaction of the luting cement with dentin, which depends on the wetting ability and the extent to which these adhesives penetrate the tooth surface. The wetting ability of the luting cement is influenced by the surface topography of the prepared tooth surface. Smear layer, containing denatured collagen fibrils and hydroxyapatite crystals, is formed on the tooth surface following rotary instrumentation. Its thickness on prepared dentin surface is influenced by the types of burs used. Coarse diamond bur produced a thicker smear layer, which can interfere bonding with luting cement. Hence, information on the effect of different burs on bond strength of self-etch resin luting cement to dentin is essential for the clinical success of indirect restorations. 2. While many studies have compared the tensile or shear bond strength of resin luting cements to dentin, very few have assessed the bond strength under simulated pulpal pressure. The presence of a positive pulpal pressure may dilute the luting cement when bonding is performed on deep dentin and result in a weaker adhesive interface. Thus, in order to predict the clinical behaviour of these cements on vital teeth, we plan to examine the effect of simulated pulpal pressure on permeability and bond strength of luting cements to dentin. 3. Water sorption from underlying dentin is further facilitated by the increased concentration of hydrophilic ionic resin monomers in self-etch luting cement and their longer setting time. Recent studies have indicated that pressure application during the setting of resin cement suppress the absorption of water and improves bond strength. However, it is not known whether similar improvement in bond strengths could be observed in perfused teeth. Hence, we decide to examine the effect of sustained seating pressure on bond strength of luting cement to perfused dentin. |
| Project Title: | 10th Congress of the European Academy of Paediatric Dentistry jointly with the British Society of Paediatric Dentistry (EAPD 2010) Infraoccluded primary mandibular molars do (not) erupt - an atypical outcome |
| Investigator(s): | King NM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2010 |
| Completion Date: | 06/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Anthonappa R.P. and King N.M., An unusual white lesion in a 10-month-old child, Dental Update. 2010, 37: 110-114. |
| Anthonappa R.P. and King N.M., An unusual white lesion in a 10-month-old child, Dental Update. 2010, 37: 110-114. |
| Chan Y.L., Ngan A.H.W. and King N.M., Degraded prism sheaths in the transition region of hypomineralized teeth, Journal of Dentistry. 2010, 38: 237-244. |
| Chung C.W., Yiu C.K.Y., King N.M., Hiraishi N. and Tay F.R., Effect of saliva contamination on bond strength of resin luting cements to dentin. , Journal of Dentistry. 2009, 37: 923-31. |
| Du R.Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health- and oral health-related quality of life among preschool children with cerebral palsy, Quality of Life Research. 2010, 19: 1367-1371. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Du RY, McGrath C, Yiu CKY, King NM. Health-related and oral health-related quality of life among preschool children with cerebral palsy. Best Poster Presentation , Conjoint scientific sessions – Faculty of Dentistry, the University of Hong –Peking University School of Stomatology, 16-17 November 2009. 2009. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health and oral health-related quality of life among preschool children with Cerebral Palsy, 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health-related and oral health-related quality of life among preschool children with cerebral palsy. , Conjoint Scientific Session. The University of Hong Kong-Peking University School of Stomatology, 16-17 November, 2009. p.32. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Oral health in the primary dentition of children with cerebral palsy. , 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R.C.M., Anti-microbial efficacy of 3.8% silver diamine fluoride and its effect on root dentin., Journal of Endodontics. 2010, 36:: 1026-1029. |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R., Antibacterial effect of experimental chlorhexidine-releasing polymethyl methacrylate-based root canal sealers. , Journal of Endodontics. 2009, 35: 1255-8. |
| Hiraishi N., Yiu C.K.Y., King N.M., Tagami J. and Tay F.R.C.M., Antimicrobial efficacy of 3.8% silver diamine fluoride and its effect on root dentin, Journal of Endodontics. 2010, 36(6): 1026-1029. |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R.C.M., Effect of chlorhexidine incorporation into a self-etching primer on dentine bond strength of a luting cement., Journal of Dentistry. 2010, 38: 496-502. |
| King N.M., Tsai J.S.J. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part I: Anomalies in the permanent dentition, The Open Anthropology Journal. 2010, 3: 54-64. |
| King N.M., Tsai J.S.J. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part II: Traits in the permanent dentition , The Open Anthropology Journal. 2010, 3: 71-84. |
| King N.M., Tongkoom S. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part III: Anomalies in the primary dentition, The Open Anthropology Journal. 2010, 3: 25-36. |
| King N.M., Tongkoom S. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part IV: Traits in the primary dentition, The Open Anthropology Journal. 2010, 3: 37-53. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and Responsiveness of ChineseECOHIS to Dental Treatment, 1st Hong Kong International Dental Expo and Symposium. 2010. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and responsiveness of the Chinese version of the Early Childhood Oral Health Impact Scale (ECOHIS) to dental treatment under general anaesthesia. , Conjoint Scientific Session 2009. The University of Hong Kong-Peking University School of Stomatology, 16-17 Kong November 2009. p.32. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Translation and validation of a Chinese language version of the Early Childhood Oral Health Impact Scale (ECOHIS), International Journal of Paediatric Dentistry. 2009, 19: 399-405. |
| Omer R.S.M., Anthonappa R.P. and King N.M., Determination of the optimum time for surgical removal of unerupted anterior supernumerary teeth, In: Omer RSM, Anthonappa RP, King NM, Pediatric Dentistry. 2010, 32(1): 14-20. |
| Sanares A.M.E., King N.M., Itthagarun A. and Wong H.M., Chewing gum as a medium for the delivery of anticariogenic therapeutic agents: a review , Hong Kong Dental Journal. 2009, 6: 13-22. |
| Yiu C.K.Y., Chung C.W.M., Hiraishi N., King N.M. and Tay F.R., Effect of chlorhexidine concentration on the mechanical properties of dental adhesive resins., Conjoint Scientific Session 2009. The University of Hong Kong-Peking University School of Stomatology, 16-17 November 2009. p.20. |
| Researcher : Kuo DDW |
| Project Title: | Periodontal Biotypes in Chinese Population |
| Investigator(s): | Kuo DDW, Corbet EF, Jin LJ, Leung WK, Lai SML |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 10/2008 |
| Abstract: |
| The severity of presentations in periodontal disease has been proposed to differ, among various periodontal tissue morphologic entities or "biotypes". Gingival dimensions show great intra- and inter-individual variation in the clinical manifestations and gingival biotype not only affects the dentition and the esthetic outcome of periodontal disease, but it will also affect the non-surgical and surgical post-therapy outcomes. The human gingival biotype is classified as thin-scalloped or thick-flat. According to Weisgold's (1977) classification, there are two major periodontal biotypes, and their characteristics are as follows: Thin-Scalloped • Distinct disparity between height of gingival margin on direct labial/buccal and height of gingival margin inter-proximally. • Delicate and friable soft-tissue curtain • Underlying osseous form scalloped, dehiscences and fenestrations often present. • Small amount of attached masticatory mucosa (quantitative and qualitative) . • Reacts to insult by recession. • Subtle, diminutive convexities in cervical thirds of labial/buccal surfaces. • Contact areas of adjacent teeth located toward the incisal or occlusal thirds. • Teeth triangular in shape. • Small contact areas of adjacent teeth bucco-lingually and inciso-gingivally. Thick-Flat • Not as great a disparity between height of gingival margin on direct labial/buccal surface and height of gingival margin interproximally. • Denser, more fibrotic soft-tissue curtain. • Underlying osseous form is flatter and thicker. • Large amount of attached masticatory mucosa (quantitative and qualitative). • Reacts to insult by pocket formation. • More prominent, bulbous convexities in cervical thirds of labial/buccal surfaces. • Contact areas of adjacent teeth located more toward the apical. • Teeth more square in shape. • Large contact areas of adjacent teeth bucco-lingually and inciso-gingivally Different biotypes have a tendency to respond differently during inflammation and resolution of inflammation following non-surgical and surgical interventions. In a thin-scalloped periodontium, it has been speculated that thin tissues are more vulnerable to plaque and trauma and respond with gingival recession following the non-surgical periodontal treatment (Claffey &Shanley 1986). Invasive surgical and restorative intervention result in some degree of soft-tissue recession especially in the papilla area, which may create a great esthetic concern. A thin maxillary buccal plate is predisposed to defect formation secondary to remodeling and resorption of bone after extractions and/or surgical therapy. It has been widely accepted that the clinical impression of thin biotypes being less resistant to microbial irritants appears to have a basis (Seibert & Lindhe ,1989) and that thin bio-types more readily undergo loss of periodontal attachment. On the other hand, the thick-flat periodontal biotype has the ability to resist recession, but responds to plaque accumulation and plaque retentive restorative therapy with pocket formation. This type of tissue is predisposed to forming notches and scars that can jeopardize final esthetic and functional results. Weisgold (1977),Seibert &Lindhe (1989),Olsson et al (1991) all have attempted to correlate tooth morphology and characteristics of the periodontium. A tooth with a tapered/long crown form and small proximal contact areas with highly scalloped gingival margin seems to be associated with thin biotypes. Teeth with short but wide crowns and large proximal contact areas are associated with thick periodontal biotypes. Furthermore, the degree of interproximal gingival margin fill is also dependant of the periodontal biotype. A thick periodontal biotype encourages inter-dental fill, while a thinner tissue type creates un-aesthetic hollow gingival embrasures. This is a problem when an implant is placed next to a natural tooth. It is the interproximal bone of the adjacent natural tooth that determines the presence, or absence of a papilla, not the bone around the dental implant. For thick biotypes, the papilla may be establish easily but for thin biotypes, it is difficult to recreate a papilla longer than 4 mm from the osseous crest (Jemt , 1997). The aim of this study is to describe the periodontal biotypes in the southern Chinese population. Specifically its objectives are: a) in periodontally healthy southern Chinese individuals to characterize the periodontal soft tissue appearance and width of keratinized gingiva at different locations in the oral cavity; b) correlate the gingival soft tissue appearance in relationship to probing depths, probing attachment levels and gingival recession; c) investigate maxillary central incisor shapes as they relate to gingival biotypes. |
| List of Research Outputs |
| Kuo D.D.W., Soft Tissue Management Around Implant, The Academy Of Dental Implantology, Taiwan. 2010. |
| Kuo D.D.W., Soft Tissue Management In Periodontology, Hku-pku Conjoint Program In Beijing. 2010. |
| Researcher : Lai SML |
| List of Research Outputs |
| Corbet E.F., Ho D.K.L. and Lai S.M.L., Radiographs in periodontal disease diagnosis and management, Australian Dental Journal. 2009, 54 (Suppl 1): S27-S43. |
| Lai S.M.L., Current concept in non-surgical periodontal therapy, 牙周非手术治疗的最新进展, World Universities Network Oral Health Science, Nanjing Forum 2009. 世界大学联盟口腔医学论坛，南京·2009, 2009. |
| Lai S.M.L., Implant in periodontal patient - What did we learn and what to expect?, Implant Club Hong Kong East. 2009. |
| Researcher : Lane T |
| List of Research Outputs |
| Lai Y.Y.W. and Lane T., Characteristics of Medical Research News Reported on Front Pages of Newspapers , In: Alan Ruttenberg, Science Commons, United States of America, Public Library of Science ONE. 2009, PLoS ONE 4(7): e6103. doi:10.1371/journal.pone.0006103. |
| Lai Y.Y.W. and Lane T., Sources and Coverage of Medical News on Front Pages of US Newspapers, Public Library of Science ONE. 2009, PLoS ONE 4(9): e6856. doi:10.1371/journal.pone.0006856. |
| Researcher : Lang NP |
| List of Research Outputs |
| Burkhardt R. and Lang N.P., Role of flap tension in primary wound closure of mucoperiosteal flaps: a prospective cohort study, Clinical Oral Implants Research. 2010, 21: 50-54. |
| Caneva M., Botticelli D., Salata L.A., Scombatti Souza S.L., Carvalho Cardoso L. and Lang N.P., Collagen membranes at immediate implants: a histomorphometric study in dogs, Clinical Oral Implants Research. 2010, 21: 891-897. |
| Caneva M., Salata L.A., de Souza S.S., Bressan E., Botticelli D. and Lang N.P., Hard tissue formation adjacent to implants of various size and configuration immediately placed into extraction sockets: an experimental study in dogs, Clinical Oral Implants Research. 2010, 21: 885-890. |
| Caneva M., Salata L.A., de Souza S.S., Baffone G., Lang N.P. and Botticelli D., Influence of implant positioning in extraction sockets on osseointegration: histomorphometric analyses in dogs, Clinical Oral Implants Research. 2010, 21: 43-49. |
| Chang P.C., Lang N.P. and Giannobile W.V., Evaluation of functional dynamics during osseointegration and regeneration associated with oral implants, Clinical Oral Implants Research. 2010, 21: 1-12. |
| Ferrus J., Cecchinato D., Pjetursson E.B., Lang N.P., Sanz M. and Lindhe J., Factors influencing ridge alterations following immediate implant placement into extraction sockets, Clinical Oral Implants Research. 2010, 21: 22-29. |
| Han J., Lulic M. and Lang N.P., Factors influencing resonance frequency analysis assessed by Osstell mentor during implant tissue integration: II. Implant surface modifications and implant diameter, Clin Oral Implants Res. 2010, 21: 605-611. |
| Heitz-Mayfield L.J.A. and Lang N.P., Comparative biology of chronic and aggressive periodontitis vs. peri-implantitis, Periodontology 2000. 2010, 53: 167-181. |
| Huyn-Ba G., Brägger U., Zwahlen M., Lang N.P. and Salvi G.E., Periodontal disease progression in subjects with orofacial clefts over a 25-year follow-up period, Journal of Clinical Periodontology. 2009, 36: 836-842. |
| Huynh-Ba G., Pjetursson B.E., Sanz M., Cecchinato D., Ferrus J., Lindhe J. and Lang N.P., Analysis of the socket bone wall dimensions in the upper maxilla in relation to immediate implant placement, Clinical Oral Implants Research. 2010, 21: 37-42. |
| Jensen J., Lulic M., Heitz-Mayfield L.J.A., Joss A. and Lang N.P., Nd:YAG (1064 nm) laser for the treatment of chronic periodontitis: a pilot study, Journal of Investigative and Clinical Dentistry. 2010, 1: 16-22. |
| Kuonen P., Huynh-Ba G., Krummen V.S., Stössel E.M., Röthlisberger B., Salvi G.E., Gerber J., Pjetursson B.E., Joss A. and Lang N.P., Restoration margins in young adolescents: A clinical and radiographic study of Swiss Army Recruits, Oral Health and Preventive Dentistry. 2009, 7: 377-382. |
| Lang N.P., Associate Editor, In: Lakshman P. Samaranayake, Journal of Investigative and Clinical Research. 2010. |
| Lang N.P., Cullinan M.P., Holborow D.W. and Heitz-Mayfield L.J.A., Examiner training: standardization and calibration in periodontal studies, In: Clinical Research in Oral Health. Chapter 9. Blackwell Publishing, 2010, 159-175. |
| Lang N.P., Schätzle M.A. and Löe H., Gingivitis as a risk factor in periodontal disease, Journal of Clinical Periodontology. 2009, 36: 3-8. |
| Lang N.P., Honorary membership, Australian New Zealand Academy of Periodontology ANZAP. 2010. |
| Lang N.P., Honorary membership, Danish Society for the Study of Periodontology. 2010. |
| Lang N.P. and Teo M., How do patient data affect treatment planning and prognosis?, In: Periodontology: Beyond the Pocket. (Bartold, P.M., Chung, K.M., Eds.). Chapter 11. Asian Pacific Society of Periodontology, 2010, 112-114. |
| Lang N.P. and Jepsen S., Implant surfaces and design (Working Group 4), Clinical Oral Implants Research. 2009, 20: 228-231. |
| Lang N.P. and Tonetti M.S., Peri-implantitis: etiology, pathogenesis, prevention, and therapy, In: Dental Implant Complications. (Froum, S.J., Ed.). Chapter 7. Wiley-Blackwell, 2010, 119-133. |
| Lulic M., Görög I.L., Salvi G.E., Ramseier C.A., Mattheos N. and Lang N.P., One-year outcomes of repeated adjunctive photodynamic therapy during periodontal maintenance: a proof-of-principle radomized-controlled clinical trial, Journal of Clinical Periodontology. 2009, 36: 661-666. |
| Lulic M., Leiggener Gorog I., Salvi G.E., Ramseier C.A., Mattheos N. and Lang N.P., One-year outcomes of repeated adjunctive photodynamic therapy during periodontal maintenance: a proof-of-principle randomized-controlled clinical trial, J Clin Periodontol. 2009, 36: 661-666. |
| Matarasso S., Rasperini G., Iorio Siciliano V., Salvi G.E., Lang N.P. and Aglietta M., A 10-year retrospective analysis of radiographic bone-level changes of implants supporting single-unit crowns in periodontally compromised vs. periodontally health patients, Clinical Oral Implants Research. 2010, 21: 898-903. |
| Matuliene G., Studer R., Lang N.P., Schmidlin K., Pjetursson B.E., Salvi G.E., Brägger U. and Zwahlen M., Significance of periodontal risk assessment in the recurrence of periodontitis and tooth loss, Journal of Clinical Periodontology. 2010, 37: 191-199. |
| Pjetursson B.E., Rast C., Brägger U., Schmidlin K., Zwahlen M. and Lang N.P., Maxillary sinus floor elevation using the (transalveolar) osteotome technique with or without grafting material. Part I: implant survival and patients' perception, Clinical Oral Implants Research. 2009, 20: 667-676. |
| Pjetursson B.E., Ignjatovic D., Matuliene G., Brägger U., Schmidlin K. and Lang N.P., Transalveolar maxillary sinus floor elevation using osteotomes with or without grafting material. Part II: radiographic tissue remodeling, Clinical Oral Implants Research. 2009, 20: 677-683. |
| Salvi G.E., Chiesa A.D., Kianpur P., Attström R., Schmidlin K., Zwahlen M. and Lang N.P., Clinical effects of interdental cleansing on supragingival biofilm formation and development of experimental gingivitis, Oral Health and Preventive Dentistry. 2009, 7: 383-391. |
| Salvi G.E., Franco L.M., Braun T.M., Lee A., Persson G.R., Lang N.P. and Giannobile W.V., Pro-inflammatory biomarkers during experimental gingivitis in patients with type 1 diabetes mellitus: a proof-of-concept study, Journal of Clinical Periodontology. 2010, 37: 9-16. |
| Sanz M., Cecchinato D., Ferrus J., Pjetursson E.B., Lang N.P. and Lindhe J., A prospective, randomized-controlled clinical trial to evaluate bone preservation using implants with different geometry placed into extraction sockets in the maxilla, Clinical Oral Implants Research. 2010, 21: 13-21. |
| Schmidlin K., Schnell N., Steiner S., Salvi G.E., Pjetursson B., Matuliene G., Zwahlen M., Brägger U. and Lang N.P., Complication and failure rates in patients treated for chronic periodontitis and restored with single crowns on teeth and/or implants, Clinical Oral Implants Research. 2010, 21: 550-557. |
| Schätzle M., Männchen R., Zwahlen M. and Lang N.P., Survival and failure rates of orthodontic temporary anchorage devices: a systematic review, Clinical Oral Implantology Research. 2009, 20: 1351-1359. |
| Spahni A.I., Schawalder P., Rothen B., Bosshardt D.D., Lang N.P. and Stoffel M.H., Immunohistochemical localization of RNAK, RANKL and OPG in healthy and arthritic canine elbow joints, The American College of Veterinary Surgeons. 2009, 38: 780-786. |
| Tomasi C., Sanz M., Cecchinato D., Pjetursson B., Ferrus J., Lang N.P. and Lindhe J., Bone dimensional variations at implants placed in fresh extraction sockets: a multilevel multivariate analysis, Clinical Oral Implants Research. 2010, 21: 30-36. |
| Researcher : Lee HMG |
| List of Research Outputs |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and Responsiveness of ChineseECOHIS to Dental Treatment, 1st Hong Kong International Dental Expo and Symposium. 2010. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and responsiveness of the Chinese version of the Early Childhood Oral Health Impact Scale (ECOHIS) to dental treatment under general anaesthesia. , Conjoint Scientific Session 2009. The University of Hong Kong-Peking University School of Stomatology, 16-17 Kong November 2009. p.32. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Translation and validation of a Chinese language version of the Early Childhood Oral Health Impact Scale (ECOHIS), International Journal of Paediatric Dentistry. 2009, 19: 399-405. |
| Researcher : Lee W |
| List of Research Outputs |
| Ikegami T., Wong R.W.K., Lee W., Hibino K. and Hagg E.U.O., The Hybrid Orthodontic Treatment System (HOTS), World Journal of Orthodontics. 2010, 11: 168-179. |
| Lee W. and Wong R.W.K., Hong Kong Dental Journal, The Gold Medal of the Conjoint Membership in Orthodontics Examination of the Royal College of Surgeons of Edinburgh and the College of Dental Surgeons of Hong Kong 2007 – Non-surgical orthodontic treatment of anterior open bite. 2009, 6: 103-107. |
| Researcher : Leung KCM |
| Project Title: | Phospholipase, proteinase and haemolytic activities of oral Candida albicans of patients with Sjögren’s syndrome |
| Investigator(s): | Leung KCM, Tsang PCS |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2008 |
| Abstract: |
| Sjögren’s syndrome (SS) sufferers have higher prevalence of oral Candida albicans. C. albicans is the causative agent of oral candidiasis, a condition commonly detected in subjects with SS. It may also play a significant role in caries development. Extracellular hydrolytic enzymes such as phospholipase and secreted aspartyl proteinase (Saps) are important for candidal overgrowth by their ability to facilitate adherence and tissue penetration. Haemolysin is known to be another putative virulence factor that contributes to candidal pathogenesis. The secretion of haemolysin, followed by iron acquisition, in particular, facilitates hyphal invasion in disseminated candidiasis. Few studies have investigated the secretion of both phospholipases and Saps together in C. albicans isolated from SS patients. Furthermore, to our knowledge, the secretion of haemolysin from C. albicans isolated from SS patients has not been studied previously. Objectives: 1. to determine phospholipase, Sap and haemolysin activities of C. albicans isolated from SS patients 2. to investigate any correlations between the enzyme profiles of C. albicans isolates and patients’ oral health status, sialometry and demographic characteristics |
| Project Title: | A prospective study on the effect of new complete dentures on quality of life, emotion and nutrition |
| Investigator(s): | Leung KCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2008 |
| Abstract: |
| Edentulism has a significant impact on daily living. Edentate people without teeth replacement often experience reduction in masticatory efficiency, restriction of food choice and enjoyment of food. These do not only lead to a poor nutrition status but also have a negative impact on the oral health-related quality of life and socialization. Providing edentulous patients with complete dentures allows restoration of functions and aesthetics, expands their food choice and thus may positively affect their nutrition status and oral health-related quality of life. Better socialisation may also improve their emotion and life enjoyment. To date, few studies have investigated the direct effect of teeth replacement on emotion. It is anticipated that provision of complete dentures to edentulous patients has a positive effect on the oral health-related quality of life and emotion (happiness). Objectives: 1. to assess the within-subject effect of provision of complete dentures on quality of life using Oral health impact profile- Edent (OHIP-Edent) 2. to assess the within-subject effect of provision of complete dentures on emotion using Depression Happiness Scale (DHS) 3. to measure the change of Body Mass Index (BMI) upon provision of new complete dentures |
| Project Title: | Randomized double-blind placebo-controlled trial of fluoride varnish for caries prevention in patients with Sj鐷rens syndrome' |
| Investigator(s): | Leung KCM, Mok TMY, Pow EHN, Tsang PCS |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 12/2008 |
| Abstract: |
| (1) To measure and compare the effect of a quarterly applied fluoride varnish versus a placebo varnish on caries increment in patients with Sjögren’s syndrome: (2) To assess the effect of a quarterly applied fluoride varnish versus a placebo varnish on oral microbiological status of oral mucosa, saliva and dental plaque in patients with Sjögren’s syndrome, by measuring and comparing the levels of oral yeasts and lactobacilli. |
| Project Title: | 10th Internatinal Symposium on Sjogren's Syndrome Effect of cevimeline hydrochloride on dental caries-related micro-organisms in patients with Sjögren's syndrome |
| Investigator(s): | Leung KCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 10/2009 |
| Completion Date: | 10/2009 |
| Abstract: |
| N/A |
| Project Title: | Willingness-to-accept (WTA) for dental implant treatment |
| Investigator(s): | Leung KCM, McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2009 |
| Abstract: |
| Dental implant therapy has provided promising and predictable treatment outcomes for tooth replacement. In the replacement of a single missing tooth, dental implant restores function and aesthetics, and hence improving the quality of life. Sucess of implant treatment is often measured using clinical parameters, such as implant survival and complication rate. Satisfaction with implant treatment, in the patient's perspective, is also an important parameter to inform healthcare providers how much benefit is perceived after having this intervention. It is important to understand why patient prefers an intervention and how patient rate the benefit of the intervention. Utility associated with a particular intervention measures the expected effect of undertaking the intervention on the individual's assessment of his or her well-being. Willingness to accept (WTA) is commonly used to evaluate the cost-effectiveness of a health intervention. In patients who had received treatment and experienced the outcomes, WTA represents the minimum amount they would be willing to accept to go back to their original state of health before the intervention. Objectives: 1) To investigate the WTA value of dental implant in people who had received single tooth replacement by implant therapy 2) To investigate factors affecting WTA |
| List of Research Outputs |
| Leung K.C.M., Assessment, Updates on Clinical Teaching in Oral Rehabilitation. 2010. |
| Leung K.C.M., Designs of Prostheses: Extensive Partial Dentures, Updates on Clinical Teaching in Oral Rehabilitation. 2010. |
| Leung K.C.M., McMillan A.S. and Leung W.K., Effect of cevimeline hydrocholoride on dental caries-related micro-organisms in patients with Sjogren's syndrome, 10th International Symposium on Sjogren's Syndrome. 2009, 100. |
| Leung K.C.M. and Pow E.H.N., Implant therapy for the medically compromised patients, In: Chen ZF, Research and clinical application of dental implant therapy. Beijing, People’s Military Medical Press, 2010, 83-91. |
| Leung K.C.M., Introduction to Oral Rehabilitation, Summer Camp, HKU. 2009. |
| Leung K.C.M., Overview of the OR undergraduate curriculum, Updates on Clinical Teaching in Oral Rehabilitation. 2010. |
| Pow E.H.N., Chen Z. and Leung K.C.M., Complications and their managing principles for dental implant therapy, In: Chen ZF, Research and clinical application of dental implant therapy. Beijing, People’s Military Medical Press, 2010, 235-246. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Researcher : Leung VWH |
| Project Title: | Solution ionic effects on the demineralisation of calcified hard tissue |
| Investigator(s): | Leung VWH |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 01/1994 |
| Abstract: |
| To study the aqueous solution ionic effects on the demineralisation rate of dental calcified hard tissue. |
| Project Title: | Study of the calcium-phosphate system artificial oral environments |
| Investigator(s): | Leung VWH, Darvell BW |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 01/1994 |
| Abstract: |
| In parallel with the numerical modelling work for calcium-phosphate in human saliva, direct studies of the stabilities of the calcium phosphate systems in various aqueous ionic media re being undertaken to provide experimental tests of the theoretical predictions. |
| Project Title: | Numerical modelling of the calcium-phosphate system in human saliva |
| Investigator(s): | Leung VWH, Darvell BW |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 01/1994 |
| Abstract: |
| Use of RAMESES, software designed for the solution of multiple equilibrium equations, to model the chemistry of saliva with a view to understanding the solid / solution interactions of the calcium-phosphate system. |
| Project Title: | Further development of an artificial saliva |
| Investigator(s): | Leung VWH, Darvell BW |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 01/1994 |
| Abstract: |
| To further develop an artificial saliva for the laboratory testing of the performance of materials serving in the oral environment. |
| Project Title: | Legal aspects of dentsitry |
| Investigator(s): | Leung VWH |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 07/2001 |
| Abstract: |
| To study the legal implications on the practice of dentists and the handling of dental materials by the dental profession. |
| Researcher : Leung WK |
| Project Title: | 79th General Session and Exhibition of the International Association for Dental Research Predominant Cultivable Subgingival Flora of Renal Transplant Recipients |
| Investigator(s): | Leung WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2001 |
| Abstract: |
| N/A |
| Project Title: | A randomized clinical trial using Rofecoxib as an adjunct to promote periodontal healing in non-surgical mechanical therapy on smokers |
| Investigator(s): | Leung WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 12/2003 |
| Abstract: |
| To carry out randomized clinical trial using Rofecoxib as an adjunct to promote periodontal healing in non-surgical mechanical therapy on smokers. |
| Project Title: | Lipopolysaccharide Induces Hypoxia-Inducible Factor 1 alpha in Periodontal Epithelium through Toll-like Receptor 4 |
| Investigator(s): | Leung WK, Fung ML, Xu A |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2008 |
| Abstract: |
| Background Hypoxia-inducible factor -1 (HIF-1) is a critical nuclear transcription factor responding to intracellular hypoxia. HIF-1 is composed of a constitutively expressed β-subunit (HIF-1β) and an oxygen-sensitive α-subunit (HIF-1α). During normoxia HIF-1α is a short-lived polypeptide which is hydroxylated on several proline and asparaginyl residues. This enables high-affinity binding of HIF-1α to von Hippel–Lindau tumor suppressor protein (VHL) and the follow-up degradation of HIF-1α by an ubiquitin ligase complex. Under hypoxic conditions the O2-dependent hydroxylation of HIF-1α is relaxed, leading to the accumulation of functional HIF-1 complex and subsequently the transcription of downstream genes. HIF-1 has a broad spectrum of downstream genes including vascular endothelial growth factor (VEGF), glucose transporter-1 (GLUT-1), inducible nitric oxide synthase (iNOS), transferrin and transferrin receptor, etc. Many of them are related to local vascularization and systemic oxygen transport[1]. Not only hypoxia induces HIF-1α accumulation, non-hypoxic stimuli such as inflammatory mediators, cytokines, reactive oxygen (ROS) and nitrogen (RNS) species can also activate HIF-1α even under normoxia. Lipopolysaccharide (LPS), for instance, has been reported to induce HIF-1α transcription and accumulation in phagocytes. In these cells, HIF-1α plays an essential role in cell aggregation, motility, invasiveness, and bacterial killing[2]. Conditionally knocking out HIF-1α in myeloid cell lineage results in decreased bactericidal activity with reduced production of ATP, antimicrobial peptides and tumor necrosis factor-α [3]. In addition, in a rheumatoid arthritis model, loss of HIF-1α in myeloid cells greatly inhibited the progress of joint destruction[2]. The signaling pathway by which LPS induces HIF-1α seems to be cell-specific. In hepatocytes, JNK signaling and c-Jun activation were suggested to be required for LPS-induced HIF-1α transcription[4] while in microglial cells NADPH oxidase and sp1 were reported to be involved in the same process[5]. More recently, Toll-like receptor (TLR) 4 is indicated to play a critical role in the LPS-HIF-1α signal pathway in human myeloid monocytic leukaemia cells[6]. TLR4 is a principal relay for LPS signaling. It works synergistically with CD14 to trigger a complex signal-transduction cascade, which ultimately leads to activation of the transcription factor nuclear factor-κB (NF-κB) and increased transcription of pro-inflammatory cytokines such as interleukin-6 (IL-6) and tumor-necrosis factor (TNF)[7]. We previously reported that TLR4 is up-regulated in diseased periodontal tissue, implicating a potential signaling pathway from LPS, CD14 to TLR4 in gingiva [8-10]. This pathway might account for the induction of various pro-inflammatory cytokines by LPS in periodontal epithelium and provide a mechanism for infectious periodontal destruction. Keratinocytes, especially those in junctional and sulcular epithelium, are important elements for maintaining the well-being of periodontal tissue. Firatly, bacterial colonization always initiate in gingival sulcus. Histologically, inflammatory infiltration can be found even in clinically healthy gingiva, especially in the proximity of junctional and sulcular epithelium. Secondly, non-keratinized epithelium lining the gingival sulcus is a relatively weaker barrier against invading bacterial pathogens, compared to the keratinized epithelium covering the rest of gingiva. In fact, changes in junctional and sulcular epithelium in response to bacterial challenge are commonly regarded as the first signs of gingivitis and periodontitis, in which LPS is widely thought to play a major role[11]. Recently we found that human periodontal keratinocytes express HIF-1α in vivo. HIF-1α protein can be immunohistochemically identified in gingival, sulcular and junctional epithelium. In addition, active periodontal inflammation enhances the expression of HIF-1α and its downstream gene VEGF (Unpublished data). These new finding inspires us to hypothesize that LPS induces HIF-1α in periodontal keratinocytes through Toll-like receptor 4 pathway and plays a role in the pathogenesis of periodontal inflammation. Project Objectives 1. To investigate the mRNA and protein expression of HIF-1α and its downstream genes VEGF, iNOS and GLUT-1 in an oral keratinocyte linkage in response to LPS stimulus; 2. To confirm that LPS-induced HIF-1α is biologically functional in the oral keratinocyte lineage; 3. To examine the LPS-induced HIF-1α activity in keratinocytes from wild or TLR4 nonsense mutant mice; 4. To topically apply LPS in the gingival sulcus of TLR4-mutant or wild-type rats and monitor the induced periodontal destruction and HIF-1α expression. Key Issues 1. Does LPS induce HIF-1α through a mechanism similar to hypoxia by relaxing VHL-mediated degradation? 2. Among 10 Toll-like receptors, which one serves the major pathway to induce the expression of HIF-1α in response to LPS? 3. Do HIF-1α and its downstream genes play a role in the pathogenesis of periodontal inflammation? References 1. Schofield CJ, Ratcliffe PJ (2004). Nat Rev Mol Cell Biol 5: 343-354 2. Cramer T, Yamanishi Y, Clausen BE, et al. (2003). Cell 112: 645-657 3. Peyssonnaux C, Datta V, Cramer T, et al. (2005). J Clin Invest 115: 1806-1815 4. Kim HY, Kim YH, Nam BH, et al. (2007). Exp Cell Res 313: 1866-1876 5. Oh YT, Lee JY, Yoon H, et al. (2008. Neurosci Lett 431: 155-160 6. Sumbayev VV (2008). FEBS Lett 582: 319-326 7. Miller SI, Ernst RK, Bader MW (2005). Nat Rev Microbiol 3: 36-46 8. Ren L, Jin L, Leung WK (2004). J Periodontal Res 39: 242-248 9. Ren L, Leung WK, Darveau RP, et al. (2005). J Periodontol 76: 1950-1959 10. Ren L, Leung WK, Loo TW, et al. (2005). J Periodontal Res 40: 407-416 11. Newman MG, Takei H, Klokkevold PR, et al. (2006) Carranza’s Clinical Periodontology, 10th Edition edn. Saunders |
| Project Title: | The effect of LPS/TLR4-induced HIF-1α on Collagen I Metabolism by Mice Gingival Fibroblasts |
| Investigator(s): | Leung WK, Fung ML, Xu A |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2010 |
| Abstract: |
| Periodontitis is one of the major causes for tooth mortality. It is a chronic infectious disease caused by gram-negative anaerobic bacteria. A characteristic of periodontitis is that it results in progressive breakdown of periodontal tissue, during which lipopolysaccharide (LPS) from gram-negative bacteria through interaction with toll-like receptor 4 (TLR4) of host cells is considered to be a key process which tissue destruction is initiated. C3H/HeJ mice, which are LPS-nonresponsive because of their missense-mutant TLR4 gene, were found to suffer significantly less periodontal bone loss in response to local LPS injection, compared to wild-type C3H/HeOuJ mice[1]. However, the precise mechanism underlining the LPS-induced extracellular matrix (ECM) breakdown is still not completely understood. Hypoxia-inducible factor -1 (HIF-1) is a critical nuclear transcription factor responding to intracellular hypoxia. HIF-1 is composed of a constitutively expressed β-subunit (HIF-1β) and an oxygen-sensitive α-subunit (HIF-1α). During normoxia HIF-1α is a short-lived polypeptide which is hydroxylated on several proline and asparaginyl residues. This enables high-affinity binding of HIF-1α to von Hippel–Lindau tumor suppressor protein (VHL) and the follow-up degradation of HIF-1α by an ubiquitin ligase complex. Under hypoxic conditions the O2-dependent hydroxylation of HIF-1α is relaxed, leading to the accumulation of functional HIF-1 complex and subsequently the transcription of downstream genes[2]. Not only hypoxia induces HIF-1α accumulation. Recently our study found that LPS through TLR4 and CD14 causes nuclear accumulation and bio-activity of HIF-1α polypeptide in human gingival fibroblasts. Furthermore, immunohistochemical study revealed that significantly more gingival fibroblasts express HIF-1α in nuclei during periodontitis compared to healthy tissue (unpublished data). HIF-1α might play a role in extra-cellular matrix (ECM) metabolism. Hypoxia, the HIF-1α’s most common inducer, has been found to promote collagen synthesis in various cell types[3, 4]. Recent studies further identified several ECM-turnover-relating proteins as HIF-1α’s downstream genes, including collagen prolyl 4-hydroxylase (C-P4H, a pivotal enzyme in collagen triple helix formation[5]), some matrix metalloproteinases (MMPs)[6] and certain tissue inhibitors of metalloproteinases (TIMPs)[4]. Based on the aforementioned information as well as our own research finding, we hypothesize that LPS/TLR4-induced HIF-1α regulates collagen metabolism in gingival fibroblasts. References 1. Nakamura, H., et al., Lack of Toll-like receptor 4 decreases lipopolysaccharide-induced bone resorption in C3H/HeJ mice in vivo. Oral Microbiol Immunol, 2008. 23(3): p. 190-5. 2. Semenza, G.L., Targeting HIF-1 for cancer therapy. Nat Rev Cancer, 2003. 3(10): p. 721-32. 3. Fahling, M., et al., Regulation of collagen prolyl 4-hydroxylase and matrix metalloproteinases in fibrosarcoma cells by hypoxia. Comp Biochem Physiol C Toxicol Pharmacol, 2004. 139(1-3): p. 119-26. 4. Karakiulakis, G., et al., Cell type-specific effect of hypoxia and platelet-derived growth factor-BB on extracellular matrix turnover and its consequences for lung remodeling. J Biol Chem, 2007. 282(2): p. 908-15. 5. Takahashi, Y., et al., Hypoxic induction of prolyl 4-hydroxylase alpha (I) in cultured cells. J Biol Chem, 2000. 275(19): p. 14139-46. 6. Ahn, J.K., et al., Role of hypoxia-inducible factor-1alpha in hypoxia-induced expressions of IL-8, MMP-1 and MMP-3 in rheumatoid fibroblast-like synoviocytes. Rheumatology (Oxford), 2008. 47(6): p. 834-9. |
| List of Research Outputs |
| Ajonuma L.C., Lu Q., Cheung B.P.K., Leung W.K., Samaranayake L.P. and Jin L.J., Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva, Cell Biology International. 2010, 34: 147-152. |
| Bridges S.M., Corbet E.F., Samaranayake L.P., Dyson J.E., Leung W.K. and McGrath C.P.J., Improving programme evaluations: The effect of curriculum review, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 37. |
| Chai L., Song Y., Zee K.Y. and Leung W.K., Single nucleotide polymorphisms of complement component 5 and periodontitis, J Periodontal Res. 2010, 45(3): 301-308. |
| Fan M.H.M., Wong K.L., Wu S., Leung W.K., Yam W.C. and Wong T.M., Preconditioning with Porphyromonas gingivalis lipopolysaccharide may confer cardioprotection and improve recovery of the electrically induced intracellular calcium transient during ischemia and reperfusion, Journal of Periodontal Research. 2010, 45: 100-108. |
| Leung K.C.M., McMillan A.S. and Leung W.K., Effect of cevimeline hydrocholoride on dental caries-related micro-organisms in patients with Sjogren's syndrome, 10th International Symposium on Sjogren's Syndrome. 2009, 100. |
| Leung W.K., Chu C.H., Mok T.M.Y. and Ng S.K.S., Periodontal status of adults with systemic sclerosis in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 655 (PAPF/APR). |
| Li J., Fung M.L., Xu A., Tsao G.S.W. and Leung W.K., Lipopolysaccharide causes hypoxia-inducible factor 1-alpha accumulation in gingival fibroblasts, 39th Annual Meeting of the American Association for Dental Research, Washington DC, 3/2010. |
| Parahitiyawa N.B., Scully C., Leung W.K., Yam W.C., Jin L.J. and Samaranayake L.P., Exploring the oral bacterial flora: current status and future directions, Oral Diseases. 2010, 16: 136-145. |
| Ren L., Jiang Z.Q., Fu Y., Leung W.K. and Jin L.J., The interplay of lipopolysaccharide-binding protein and cytokines in periodontal health and disease, Journal of Clinical Periodontology. 2009, 36: 619-626. |
| You M., Mo S., Leung W.K. and Watt R.M., Diversity of oral treponemes in patients with chronic periodontitis, Journal of Dental Research. 2009, 88 (Spec Iss B): 377 (PAPF/APR). |
| Researcher : Leung YY |
| List of Research Outputs |
| Leung Y.Y. and Cheung L.K., New approach in extraction of impacted wisdom teeth, The Hong Kong Medical Diary. 2010, 15 (3): 8-9. |
| Leung Y.Y. and Cheung L.K., Safety of coronectomy versus excision of wisdom teeth: a randomized controlled trial, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2009, 108(6): 821-7. |
| Researcher : Li J |
| List of Research Outputs |
| Li J., Fung M.L., Xu A., Tsao G.S.W. and Leung W.K., Lipopolysaccharide causes hypoxia-inducible factor 1-alpha accumulation in gingival fibroblasts, 39th Annual Meeting of the American Association for Dental Research, Washington DC, 3/2010. |
| Researcher : Li KY |
| List of Research Outputs |
| Li K.Y., Wong M.C.M., Lam K.F. and Schwarz E., Marginal approach in analyzing sequential cross-sectional oral health survey data, Journal of Dental Research. 2009, 88 (Spec Iss B): 311 (PAPF/APR). |
| Liu J.K.S., Pang H.H., Li K.Y., Cheung C.K.L., Fung J., Ho R.C.Y., Lam A.K.Y., Lee C.T.Y., Ng A.K.C., Sia A.O.N., Wong J.C.L. and Yung A.K.C., The dental product advertisement in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 168: 1-68. |
| Researcher : Li TKL |
| List of Research Outputs |
| MacDonald-Jankowski D.S. and Li T.K.L., Orthokeratinized odontogenic cyst in a Hong Kong community: the clinical and radiological features, Dentomaxillofacial Radiology. 2010, 39: 240-245. |
| Siu S.C., Chu F.C.S., Li T.K.L., Chow T.W. and Deng F., Imaging modalities for preoperative assessment in dental implant therapy: an overview, Hong Kong Dental Journal. 2010, 7(1): 23-30. |
| Researcher : Li X |
| List of Research Outputs |
| Li X., Tse H.F., Yiu K.H., Jia N., Chen H., Li L.S.W. and Jin L.J., Increased levels of circulating endothelial progenitor cells in subjects with moderate to severe chronic periodontitis, Journal of Clinical Periodontology. 2009, 36: 933-939. |
| Li X., Tse H.F., Li L.S.W. and Jin L.J., Salivary MIF is associated with gingival inflammation and periodontopathogens, Journal of Dental Research. 2009, 88 (Spec Iss B): 288 (PAPF/APR). |
| Researcher : Liang R |
| List of Research Outputs |
| Lo E.C.M., Xiao Y., Chu C.H. and Liang R., Preventing fissure caries by sealants and fluorides - 30-month results, Journal of Dental Research. 2009, 88 (Spec Iss C): 127 (WCPD). |
| Researcher : Lin HC |
| List of Research Outputs |
| Lin H.C., Lai Z.-.Y. and Lo E.C.M., NCCL and its influencing factors in Chinese elderly, Journal of Dental Research. 2009, 88 (Spec Iss B): 305 (PAPF/APR). |
| Liu B., Lo E.C.M. and Lin H.C., Preventing fissure caries by sealant and fluorides - 12-month results, Journal of Dental Research. 2009, 88 (Spec Iss B): 272 (PAPF/APR). |
| Lo E.C.M., Liu B. and Lin H.C., Cost of fissure caries prevention by sealants and fluorides, Journal of Dental Research. 2009, 88 (Spec Iss B): 271 (PAPF/APR). |
| Zhou Y., Lin H.C. and Lo E.C.M., Life course approach in assessment of ECC in 2-year old children, Journal of Dental Research. 2009, 88 (Spec Iss B): 5 (PAPF/APR). |
| Researcher : Ling JYK |
| List of Research Outputs |
| Ling J.Y.K. and Wong R.W.K., Incisor Winging in Chinese, The Open Anthropology Journal 2010. 2010, 3: 8-11. |
| Ling J.Y.K. and Wong R.W.K., Molar cusps in Southern Chinese, The Open Anthropology Journal 2010. 2010, 3: 16-19. |
| Researcher : Liu B |
| List of Research Outputs |
| Liu B., Lo E.C.M. and Lin H.C., Preventing fissure caries by sealant and fluorides - 12-month results, Journal of Dental Research. 2009, 88 (Spec Iss B): 272 (PAPF/APR). |
| Lo E.C.M., Liu B. and Lin H.C., Cost of fissure caries prevention by sealants and fluorides, Journal of Dental Research. 2009, 88 (Spec Iss B): 271 (PAPF/APR). |
| Researcher : Liu JKS |
| List of Research Outputs |
| Liu J.K.S., Pang H.H., Li K.Y., Cheung C.K.L., Fung J., Ho R.C.Y., Lam A.K.Y., Lee C.T.Y., Ng A.K.C., Sia A.O.N., Wong J.C.L. and Yung A.K.C., The dental product advertisement in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 168: 1-68. |
| Researcher : Lo ECM |
| Project Title: | Outstanding Research Student Supervisor Award 2006-2007 |
| Investigator(s): | Lo ECM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Outstanding Research Student Supervisor Award |
| Start Date: | 11/2007 |
| Abstract: |
| The Outstanding Research Student Supervisor Award of The University of Hong Kong is to accord recognition to supervisors of research postgraduate students who, through their highly effective supervisory guidance, have particularly benefitted their students in the pursuit of research excellence. |
| Project Title: | A randomized controlled trial on the effectiveness of fissure sealant and topical fluoride application in preventing dental caries |
| Investigator(s): | Lo ECM, Chu CH |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2008 |
| Abstract: |
| (1) To describe longitudinally the effectiveness of (a) one-time placement of resin fissure sealant, (b) semi-annual topical applications of sodium fluoride (NaF) varnish, and (c) annual topical applications of silver diamine fluoride (SDF) solution, in a school-based programme to prevent dental caries in the pits and fissures of permanent first molars in Chinese school children; (2) to compare the cost-effectiveness of the above three caries prevention methods. |
| Project Title: | Community dental care service for disadvantaged population groups |
| Investigator(s): | Lo ECM, Dyson JE, Chu CH |
| Department: | Dental Faculty |
| Source(s) of Funding: | S.K. Yee Medical Foundation - General Award |
| Start Date: | 01/2009 |
| Abstract: |
| To provide the necessary dental preventive and curative treatment s to various disadvantaged population groups (those with special social, financial, and problems) in Hong Kong who have limited access to dental care; to improve the health and qualtiy of life os the disadvantaged population groups by removing pain and diseases of dental origin; to provide clinical training to dentists who are pursuing the training pathway to be specialist in community dentistry, thus benefiting the disadvantage population groups in the long-term through the services of community dentists with enhanced moivation and skills. |
| Project Title: | 9th World Congress on Preventive Dentistry Preventing fissure caries by sealants and fluorides - 30-month results |
| Investigator(s): | Lo ECM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 09/2009 |
| Completion Date: | 09/2009 |
| Abstract: |
| N/A |
| Project Title: | Effectiveness of training in parental toothbrushing and fluoride varnish in preventing early childhood caries |
| Investigator(s): | Lo ECM, Chu CH, Wong MCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2010 |
| Abstract: |
| 1) To describe the effectiveness of hands-on training in parental toothbrushing, reinforced by home visits every 6 months, in preventing dental caries in young children; 2) To describe the effectiveness of semi-annual application of 5% sodium fluoride varnish in addition to hands-on training in parental toothbrushing in preventing dental caries in young children. |
| List of Research Outputs |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience - Journal Based Learning and Fundamentals of Dental Research – a pilot programme, the 6th International Conference on PBL in Dentistry. Hong Kong, 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience-journal based learning and fundamentals of dental research-a pilot programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.26. |
| Bridges S.M. and Lo E.C.M., PBL Programme design - past, present and future, Faculty of Education, Division of Speech & Hearing Sciences, The University of Hong Kong. 2009. |
| Chee C., Ho K.W., Yau S.W., Chu C.H. and Lo E.C.M., Oral hygiene and gingival status of Hong Kong pre-school children, Journal of Dental Research. 2009, 88 (Spec Iss B): 656 (PAPF/APR). |
| Chu C.H., Lo E.C.M. and You D.S.H., Clinical diagnosis of fissure caries with conventional and laser-induced fluorescence techniques, Lasers Medical Science. 2010, 25: 355-362. |
| Chu C.H. and Lo E.C.M., Dentin hypersensitivity: a review, Hong Kong Dental Journal. 2010, 7: 15-22. |
| Chu C.H., Pang K.K.L. and Lo E.C.M., Dietary behavior and knowledge of dental erosion among Chinese adults, BMC Oral Health. 2010, 10: 13. |
| Chu C.H., Mei L., Lo E.C.M., Seneviratne C.J. and Yip H.K., Effects of silver diamine fluoride on mono-species cariogenic biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 363 (PAPF/APR). |
| Chu C.H. and Lo E.C.M., Outreach caries control service for kindergarten children in Hong Kong, In: Programme book of Health Research Symposium 2010: Improving Health and Recognising Excellence. 2010, Ab01: p75. |
| Chu C.H. and Lo E.C.M., Outreach caries control service for kindergarten children in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss C): 47 (WCPD). |
| Chu C.H., Mei L. and Lo E.C.M., Use of fluorides in dental caries management, General Dentistry. 2010, 58: 37-43. |
| Gao X., Seneviratne C.J., Lo E.C.M., Chu C.H. and Samaranayake L.P., Novel and conventional assays for assessing risk of dental caries, In: Programme book of Health Research Symposium 2010: Improving Health and Recognising Excellence. 2010, Ab56: p75. |
| Ho P.L., Liu H.N., Lam C., Chu C.H. and Lo E.C.M., Occlusal featurs of primary dentition in Chinese preschool children, Journal of Dental Research. 2009, 88 (Spec Iss B): 601 (PAPF/APR). |
| Lin H.C., Lai Z.-.Y. and Lo E.C.M., NCCL and its influencing factors in Chinese elderly, Journal of Dental Research. 2009, 88 (Spec Iss B): 305 (PAPF/APR). |
| Liu B., Lo E.C.M. and Lin H.C., Preventing fissure caries by sealant and fluorides - 12-month results, Journal of Dental Research. 2009, 88 (Spec Iss B): 272 (PAPF/APR). |
| Lo E.C.M. and Gao X., Caries risk assessment - practical procedures beyond clinical impression, Oral Health and Science Seminar, Faculty of Dentistry, University of Hong Kong . 2010. |
| Lo E.C.M., Zhi Q. and Itthagarun A., Comparing two quantitative methods for studying remineralization of artificial caries, Journal of Dentistry. 2010, 38: 352-359. |
| Lo E.C.M., Liu B. and Lin H.C., Cost of fissure caries prevention by sealants and fluorides, Journal of Dental Research. 2009, 88 (Spec Iss B): 271 (PAPF/APR). |
| Lo E.C.M., Gao X., Lui K.C., Chan B.K.L., Chan Y.H., Cheung S.S., Hung Y.W., Lau K.H.Y., Man W.H.C. and Wu K.W., Oral health status of asthmatic preschoolers in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 167: 1-37. |
| Lo E.C.M., Gao X., Lui K.C., Chan B.K.L., Chan Y.H., Cheung S.S., Hung Y.W., Lau K.H.Y., Man W.H.C. and Wu K.W., Oral-B Prize in Community Oral Health, University of Hong Kong. 2010. |
| Lo E.C.M., Xiao Y., Chu C.H. and Liang R., Preventing fissure caries by sealants and fluorides - 30-month results, Journal of Dental Research. 2009, 88 (Spec Iss C): 127 (WCPD). |
| Lu H.X., Wong M.C.M., Lo E.C.M. and McGrath C.P.J., Factors associated with oral health from childhood to early adulthood, Journal of Dental Research. 2009, 88 (Spec Iss B): 313 (PAPF/APR). |
| Lui W.Y., Sum C.K., Chan H.W.L., Chu C.H. and Lo E.C.M., Dental caries status of preschool children in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 1 (PAPF/APR). |
| McGrath C.P.J., Zhang W. and Lo E.C.M., A review of the effectiveness of oral health promotion activities among elderly people, Gerodontology. 2009, 26: 85-96. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Action of Silver Diamine Fluoride on Dentin Caries [Abstract], First Hong Kong International Dental Expo and Symposium; Hong Kong Dental Association. 2010, In Abstracts of papers: 84. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Effects of multi-species biofilm dentin caries-like lesions in artificial mouth, Journal of Dental Research. 2010, 89 (Spec Iss A): 1393 in AADR 39th Annual Meeting. |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., Effects of silver-diamine fluoride on caries-like lesion and cariogenic biofilm, Journal of Dental Research. 2009, 88 (Spec Iss B): 40 (PAPF/APR). |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., International Association for Dental Research / Unilever Hatton Travel Award for M.Lei (co-author), 23nd Annual Scientific Meeting, Southeast Asian Division of International Association for Dental Research 2009. . 2009. |
| Rong W.S. and Lo E.C.M., Effect of providing outreach oral health care to institutionalised elders in Hong Kong, Oral Health and Preventive Dentistry. 2009, 7: 261-267. |
| Tan H., Lo E.C.M., Luo Y., Dyson J.E. and Corbet E.F., Prevention of root caries in Chinese institutionalized elders, Journal of Dental Research. 2009, 88 (Spec Iss C): 129 (WCPD). |
| Wong M.C.M., Lu H.X., Lo E.C.M. and McGrath C.P.J., Oral health status of young adults in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 308 (PAPF/APR). |
| Wong M.C.M., Glenny A.M., Tsang B.W.K., Lo E.C.M., Worthington H.V. and Marinho V.C.C., Systematic review on topical fluoride as a cause of dental fluorosis in children, 4th International Meeting of Methodological Issues in Oral Health Research. 2010. |
| Wong M.C.M., Glenny A.-.M., Tsang B.W.K., Lo E.C.M., Worthington H.V. and Marinho V.C.C., Topical fluoride as a cause of dental fluorosis in children, Cochrane Database of Systematic Reviews. 2010, Issue 1: 1-32. |
| Wu D., Lo E.C.M., Zhang Y. and Chu C.H., Oral health and hygiene status of Hong Kong kindergarten children, Journal of Dental Research. 2009, 88 (Spec Iss B): 310 (PAPF/APR). |
| Zhang W., McGrath C.P.J. and Lo E.C.M., A comparison of root caries diagnosis based on visual-tactile criteria and DIAGNOdent in vivo, Journal of Dentistry. 2009, 37: 509-513. |
| Zhou Y., Lin H.C. and Lo E.C.M., Life course approach in assessment of ECC in 2-year old children, Journal of Dental Research. 2009, 88 (Spec Iss B): 5 (PAPF/APR). |
| Researcher : Lo J |
| Project Title: | Oral versus intravenous antibiotic treatment after bimaxillary orthognathic surgery - a prospective, randomized, double-blind, placebo-controlled clinical trial |
| Investigator(s): | Lo J, Zwahlen RA |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 07/2009 |
| Abstract: |
| The primary objective of this study is to establish evidence based protocol for the regimen of the postoperative antibiotic treatment in patients after bimaxillary orthognathic surgery. Specific objectives: 1.To investigate whether oral antibiotic treatment is as effective as intravenous antibiotic treatment in preventing infection after bimaxillary osteotomies. 2. To evaluate whether oral antibiotic treatment could substitute intravenous antibiotic treatment, being both safe and more cost-effective. 3. To determine the prevalence of infection after orthognathic surgery under antibiotic treatment. |
| List of Research Outputs |
| Cheung L.K., Chua H.D.P., Hariri F., Lo J., Ow A. and Zheng L., Distraction Osteogenesis (Chapter 48), Oral and Maxillofacial Surgery, edited by Lars Andersson, Karl-Erik Kahnberg and M. Anthony Pogrel. Wiley-Blackwell, 2010, 1027 - 1059. |
| Cheung L.K. and Lo J., Endoscope-assisted rigid fixation for intraoral vertical subsigmoid osteotomy: a preliminary clinical study, Journal of Oral and Maxillofacial Surgery. 2010, 68: 8-14. |
| Jayaratne Y.S.N., Lo J., Zwahlen R.A. and Cheung L.K., 3-D Photogrammetry for Surgical Planning of Tissue Expansion in Hemifacial Microsomia., Head Neck. PMID 19862829, 2009. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Computer Aided Maxillofacial Surgery: An Update (accepted), Surgical Innovations. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Three-dimensional color maps: a novel tool for assessing craniofacial changes., Surg Innov. 2010. |
| Lo J., Classification of the defects in the face and jaw, with its clinical problems, AOCMF Focused Seminar on Distraction Bone Transplantation in Reconstruction of Jaws. 2010. |
| Lo J., Computer-assisted planning in orall & maxillofacial surgery, Oral Health Seminar. 2010. |
| Lo J., Distraction in TMJ reconstruction, Craniofacial Distraction course - Malaysian Association of Oral & Maxillofacial Surgeons. 2009. |
| Lo J., Fang D.T.S. and Cheung L.K., Long term Clinical Analysis of Immediate Implant in Aesthetic Zone, Annual Meeting of Philippine Academy of Implant Dentistry. 2009. |
| Lo J., Options of Mandibular Reconstruction: non-vascularized bone graft vs vascularized graft, AOCMF Focused Seminar on Distraction Bone Transplantation in Reconstruction of Jaws. Tokyo, Japan, 2010. |
| Lo J., Xia J.J., Zwahlen R.A. and Cheung L.K., Surgical navigation in correction of hemimandibular hyperplasia: a new treatment strategy, J Oral Maxillofac Surg. 2010, 68: 1444-50. |
| Lo J., Surgical planning in maxillo-mandibular reconstruction: stereomodel vs virtual models, AOCMF Focused Seminar on Distraction Bone Transplantation in Reconstruction of Jaws. Tokyo, Japan, 2010. |
| Lo J., Yau Y.Y. and Cheung L.K., The use of skeletal of skeletal scintigraphy in the assessment of mandibular asymmetry, Conjoint Scientific Meeting of HKU and Peking University . 2009. |
| Tam C.K., Luk W.K., Pow E.H.N., Lo J. and Cheung L.K., Immediate Implant-Supported Auricular Prosthesis Using Surgical Navigation and CAD/CAM Technology - An In Vitro Trial, 9th Meeting of International Congress on Maxillofacial Rehabilitation. 2010. |
| Researcher : Loo TY |
| List of Research Outputs |
| Loo T.Y., Jin L.J., Cheung M.N.B. and Dou Y.D., Detection of Bacteroides forsythus and Porphyromonas gingivalis in infected root canals during periapical periodontitis by 16S rDNA, African Journal of Biotechnology. 2009, 8: 2021-2026. |
| Loo T.Y., Jin L.J., Cheung M.N.B. and Wang M., The impact of diabetes on the success of dental implants and periodontal healing, African Journal of Biotechnology. 2009, 8: 5122-5127. |
| Loo T.Y., Jin L.J., Cheung M.N.B., Dou Y.D. and Chen J.P., Polistes olivaceous decreases biotic surface colonization, African Journal of Biotechnology. 2009, 8: 7094-7100. |
| Researcher : Lou Y |
| List of Research Outputs |
| Lou Y., Botelho M.G. and Darvell B.W., The Effect of Silver Diammine Fluoride on Demineralized Enamel and Dentine, European Organisation for Caries Research, Budapest, Hungary.. ORCA, 2009, 56th ORCA Congress: Abstract 89. |
| Researcher : Low DCY |
| List of Research Outputs |
| Low D.C.Y., Differing expansion contributions of three investment materials used for casting titanium, Journal of Prosthodontics. 2009, 18: 444-449. |
| Low D.C.Y., Invited speaker for "Obturation of the cleaned and shaped root canal system" and chair moderator, The 2009 Asian Endodontic Forum, Qingdao, China, 6-9 September 2009. |
| Low D.C.Y., Invited speaker for monthly half day lectures and workshops on "Contemporary endodontic therapy - what is this all about?" for Jul 2009- Jun 2010 (total 12 workshops), Dentsply Asia, Hong Kong. 2010. |
| Researcher : Lu HX |
| List of Research Outputs |
| Lu H.X., Wong M.C.M., Lo E.C.M. and McGrath C.P.J., Factors associated with oral health from childhood to early adulthood, Journal of Dental Research. 2009, 88 (Spec Iss B): 313 (PAPF/APR). |
| Wong M.C.M., Lu H.X., Lo E.C.M. and McGrath C.P.J., Oral health status of young adults in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 308 (PAPF/APR). |
| Wong M.C.M., Lu H.X., Chan K.F., Chan T.K., Chung W.Y., Leung M.Y., Mak Y.K., Pu Y.C., Wong S.S.S., Wong W.Y. and Yeung H.W., Smoking cessation in dental practices - The perspectives of the dentists in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 164: 1-43. |
| Researcher : Lu Q |
| Project Title: | Molecular mechanisms regulating innate immune responses of oral epithelial cells to Candida albicans infection |
| Investigator(s): | Lu Q, Jin LJ, Samaranayake LP, Seneviratne CJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2008 |
| Abstract: |
| Purpose of this project: 1. to investigate the innate immune responses of oral epithelial cells in C. albicans infection; 2. to investigate the innate immune responses of oral epithelial cells to C. albicans virulence factors; 3. to investigate the possible involvements of pattern recognition receptors and protease-activated receptors in the recognition of C. albicans as well as in the regulation of the downstream innate immune responses in oral epithelial cells. Key issues: Oral epithelium, the first barrier of oral mucosa, is constantly challenged by a variety of oral microorganisms. Apart from the physical barrier function, oral epithelium has recently been recognized to provide protection against microbial invasion by eliciting a vast array of innate immune responses. The importance of host innate immune response has become clear only in the past decades when the innate immune system has been known to specifically recognize various classes of microorganisms and initiate and modulate the subsequent adaptive responses through its interaction with T cells or dendritic cells (Yang et al., 1999). A number of innate immune molecules have been identified to participate in oral epithelial defense mechanism, such as pattern recognition receptors, cytokines, and antimicrobial peptides or proteins. These molecules are usually functioning cooperatively and none of them is mutually exclusive. Candida is a ubiquitous commensal that often colonizes the oral cavity, gastrointestinal tract, vagina and skin of healthy individuals. It becomes pathogenic and causes candidal infection when the balance of the normal flora is disrupted or host immune defenses are compromised. Candidal infection, termed “candidiasis”, is a problem of growing clinical importance, as the incidence of this infection has increased dramatically over the past two to three decades. Although candidiasis is frequently seen in superficial mucosal surfaces such as oral mucosa, it can lead to serious clinical complications in immunocompromosed patient populations such as HIV/AIDS, organ transplant recipients. Among the more than 150 Candida species, C. albicans is generally considered to be the most pathogenic one. It has several widely studied virulence factors, including hyphal formation, surface recognition molecules, phenotypic switching, and extracellular hydrolytic enzyme production. Problems being addressed: A defined and balanced immunomodulatory response is crucial for the protection of mucosal surfaces against candidal assault. It is known that oral epithelium responses to candidal infection by producing a panel of cytokines and antimicrobial peptides. Candida infected reconstituted human oral epithelia has been shown to secrete an array of cytokines, such as interleukin-8 (IL-8), granulocyte-macrophages colony-stimulating factor (GM-CSF), IL-1alpha, IL-1beta, and tumor necrosis factor alpha (TNF-alpha) (Schaller et al., 2002). Further, a correlation between the virulence potentials of different Candida strains and different cytokine expression patterns has also been observed (Schaller et al., 2002). Our previous studies showed that oral epithelium is capable of recognizing the early invasion of C. albicans and responding promptly by producing antimicrobial peptides human beta-defensins (hBDs) and cytokine IL-1ra, whereas during the latter stages of infection, certain yet unrecognized factors in C. albicans appear to have the ability to paralyze the host surveillance system by inhibiting hBDs mRNA and IL-1ra expression (Lu et al., 2006; Jayatilake et al., 2007). Therefore, this project is essentially an extension of our previous work. In the present study, we intend to use novel strategies and different dimensions to obtain a holistic view of Candida and epithelial interaction. Further, we will also look into the immune response of oral epithelial cells against the well known candidal virulence factors to get a deeper insight of the latter mechanism. Until recently, little was known about the ways in which oral epithelial cells recognized C. albicans as a pathogenic microorganism, or how the fungal–keratinocyte interaction triggers an inflammatory response. Two major classes of pattern recognition receptors (PRRs) in human cells, toll-like receptors (TLRs) and C-type lectin receptors (CLRs), have been implicated in the recognition of C. albicans cell wall components. For example, TLR-2 recognizes phospholipomannan (Jouault, et al., 2003) and TLR4 recognizes O‑linked mannans (Netea et al., 2006); dectin 1 recognizes β-glucans, whereas the macrophage mannose receptor and dendritic cell receptor DC-SIGN recognize N-linked mannans. However, most of these studies examined immune cells or were based on animal studies, and few data suggested whether or how these PRRs were implicated in the Candida-oral epithelial cell interaction. Further, given that hydrolytic enzyme production is one of the major virulence factors of C. albicans, it will be interesting to examine whether protease-activated receptors, a group of G-protein-coupled receptors that mediate cellular response to extracellular proteinases, participate in the pathogenic activity of C. albicans in oral epithelia. Therefore, this project will investigate the involvement of these receptors in the recognition of C. albicans as well as their roles in the activation of the downstream host responses. References: Jayatilake J. A. et al. IL-1alpha, IL-1ra and IL-8 are differentially induced by Candida in experimental oral candidiasis. 13:426-433 (2007). Jouault, T. et al. Candida albicans phospholipomannan is sensed through Toll-like receptors. J Infect Dis. 188:165–172 (2003). Lu, Q. et al. Hyphal invasion of Candida albicans inhibits the expression of human beta-defensins in experimental oral candidiasis. J Invest Dermatol. 126:2049-2056 (2006). Netea, M. G. et al. Immune sensing of Candida albicans requires cooperative recognition of mannans and glucans by lectin and Toll-like receptors. J Clin Invest. 116:1642–1650 (2006). Schaller, M. et al. Infection of human oral epithelia with Candida species induces cytokine expression correlated to the degree of virulence. J Invest Dermatol. 118:652-657 (2002). Yang, D. et al. Beta-defensins: linking innate and adaptive immunity through dendritic and T cell CCR6. Science. 286:525-528 (1999). |
| List of Research Outputs |
| Ajonuma L.C., Lu Q., Cheung B.P.K., Leung W.K., Samaranayake L.P. and Jin L.J., Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva, Cell Biology International. 2010, 34: 147-152. |
| Jin L.J., Lu Q., Darveau R.P., Samaranayake L.P. and Wang C.Y., P. gingivalis LPS modulates hBD-2 expression through TLR2 and 4, Journal of Dental Research. 2009, 88 (Spec Iss B): 749 (PAPF/APR). |
| Lu Q., Darveau R.P., Samaranayake L.P., Wang C.Y. and Jin L.J., Differential modulation of human b-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- and penta-acylated lipid A structures, Innate Immunity. 2009, 15: 325-335. |
| Researcher : Lui KC |
| List of Research Outputs |
| Lo E.C.M., Gao X., Lui K.C., Chan B.K.L., Chan Y.H., Cheung S.S., Hung Y.W., Lau K.H.Y., Man W.H.C. and Wu K.W., Oral health status of asthmatic preschoolers in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 167: 1-37. |
| Lo E.C.M., Gao X., Lui K.C., Chan B.K.L., Chan Y.H., Cheung S.S., Hung Y.W., Lau K.H.Y., Man W.H.C. and Wu K.W., Oral-B Prize in Community Oral Health, University of Hong Kong. 2010. |
| Researcher : Luk WK |
| List of Research Outputs |
| Tam C.K., Luk W.K., Pow E.H.N., Lo J. and Cheung L.K., Immediate Implant-Supported Auricular Prosthesis Using Surgical Navigation and CAD/CAM Technology - An In Vitro Trial, 9th Meeting of International Congress on Maxillofacial Rehabilitation. 2010. |
| Researcher : Lulic M |
| List of Research Outputs |
| Crail J., Lahtinen A., Beck-Mannagetta J., Benzian H., Enmarks B., Jenner T., Knevel R., Lulic M. and Wickholm S., Role and models for compensation of tobacco use prevention and cessation by oral health professionals, Int Dent J. 2010, 60: 73-79. |
| Han J., Lulic M. and Lang N.P., Factors influencing resonance frequency analysis assessed by Osstell mentor during implant tissue integration: II. Implant surface modifications and implant diameter, Clin Oral Implants Res. 2010, 21: 605-611. |
| Jensen J., Lulic M., Heitz-Mayfield L.J.A., Joss A. and Lang N.P., Nd:YAG (1064 nm) laser for the treatment of chronic periodontitis: a pilot study, Journal of Investigative and Clinical Dentistry. 2010, 1: 16-22. |
| Lulic M., Görög I.L., Salvi G.E., Ramseier C.A., Mattheos N. and Lang N.P., One-year outcomes of repeated adjunctive photodynamic therapy during periodontal maintenance: a proof-of-principle radomized-controlled clinical trial, Journal of Clinical Periodontology. 2009, 36: 661-666. |
| Lulic M., Leiggener Gorog I., Salvi G.E., Ramseier C.A., Mattheos N. and Lang N.P., One-year outcomes of repeated adjunctive photodynamic therapy during periodontal maintenance: a proof-of-principle randomized-controlled clinical trial, J Clin Periodontol. 2009, 36: 661-666. |
| Ramseier C.A., Warnakulasuriya S., Needleman I.G., Gallagher J.E., Lahtinen A., Ainamo A., Alajbeg I., Albert D., Al-Hazmi N., Antohe M.E., Beck-Mannagetta J., Benzian H., Bergstrom J., Binnie V., Bornstein M., Buchler S., Carr A., Carrassi A., Casals Peidro E., Chapple I., Compton S., Crews K., Davis J.M., Dietrich T., Enmark B., Fine J., Gallagher J., Jenner T., Forna D., Fundak A., Gyenes M., Hovius M., Jacobs A., Kinnunen T., Knevel R., Koerber A., Labella R., Lulic M., Mattheos N., McEwen A., Ohrn K., Polychronopoulou A., Preshaw P., Radley N., Rosseel J., Schoonheim-Klein M., Suvan J., Ulbricht S., Verstappen P., Walter C., Warnakulasuriya S., Wennstrom J., Wickholm S. and Zoitopoulos L., Consensus report: 2nd European workshop on tobacco use prevention and cessation for oral health professionals, Int Dent J. 2010, 60: 3-6. |
| Researcher : Luo Y |
| List of Research Outputs |
| McMillan A.S., Wong M.C.M., Zheng J., Luo Y. and Lam C.L.K., Widespread pain symptoms and psychological distress in southern Chinese with orofacial pain, Journal of Oral Rehabilitation. 2010, 37: 2-10. |
| Tan H., Lo E.C.M., Luo Y., Dyson J.E. and Corbet E.F., Prevention of root caries in Chinese institutionalized elders, Journal of Dental Research. 2009, 88 (Spec Iss C): 129 (WCPD). |
| Zheng J., McMillan A.S., Wong M.C.M., Luo Y. and Lam C.L.K., Investigation of factors associated with treatment-seeking in southern Chinese with orofacial pain, Journal of Orofacial Pain. 2010, 24: 181-188. |
| Researcher : Ma L |
| Project Title: | ISCFS XIII Biennial International Congress Influence of platelet rich plasma on nicotine compromised distraction osteogenesis |
| Investigator(s): | Ma L |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 09/2009 |
| Completion Date: | 09/2009 |
| Abstract: |
| N/A |
| Project Title: | Effect of nicotinic acetylcholine receptors on mesenchymal stem cells |
| Investigator(s): | Ma L, Cheung LK, Zheng L, Sham MH |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2009 |
| Abstract: |
| 1. To evaluate the expression of nicotinic acetylcholine receptors (nAChR) on mesenchymal stem cells (MSCs). 2. To assess the role of nAChR in the differentiation of MSCs into endothelial cells or osteoblast cells. 3. To evaluate the role of nAChR in the stimulation of osteogenic differentiation of MSCs by endothelial cells |
| List of Research Outputs |
| Ma L., Zheng L., Sham M.H. and Cheung L.K., Effect of nicotine on gene expression of angiogenic and osteogenic factors in a rabbit model of bone regeneration, Journal of Oral and Maxillofacial Surgery. 2010, 68: 777 - 781. |
| Ma L., Sham M.H., Zheng L. and Cheung L.K., Influence of low dose nicotine on bone healing. , J Trauma . 2010, In press. |
| Ma L., The influence of nicotine on angiogenesis and osteogenesis in bone regeneration (PhD Thesis, 2009). 2009. |
| Ma L., Zheng L., Sham M.H. and Cheung L.K., Uncoupled angiogenesis and osteogenesis in nicotine compromised bone healing, J Bone Miner Res . 2010, 25: 1305-13. |
| Zheng L., Ma L. and Cheung L.K., Angiogenesis is enhanced by continuous traction in rabbit mandibular distraction osteogenesis, Journal of Cranio-Maxillofacial Surgery. 2009, 37(7): p405-11. |
| Zheng L., Ma L. and Cheung L.K., Angiogenesis is enhanced by high frequency traction during distraction osteogenesis, J Craniomaxillofac Surg. 2009, 37: 405-11. |
| Zheng L., Ma L., Shi X. and Cheung L.K., Comparison of distraction osteogenesis versus costochondral graft in reconstruction of tempormandibular joint condylectomy with disc preservation, J Oral Maxillofac Surg . 2010, In press. |
| Zheng L., Ma L. and Cheung L.K., Comparison of gene expression of osteogenic factors between continuous and intermittent distraction osteogenesis in rabbit mandibular lengthening, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2009, 108 (4): 496-9. |
| Researcher : Mahindre PP |
| List of Research Outputs |
| Mahindre P.P., Cheung G.S.P. and Chang J.W.W., Micro-push-out bond strengths of an adhesively luted fiber-post subjected to simulated occlusal loads, Annual Scientific Meeting of the Faculty of Dentistry, The University of Hong Kong, Hong Kong, Nov. 2009. |
| Researcher : Matinlinna JP |
| Project Title: | Novel adhesion promotion concept for zirconia-resin bonding in dentistry |
| Investigator(s): | Matinlinna JP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 06/2009 |
| Abstract: |
| This project aims at assessing the in vitro effect of using a novel silane coupling agent, in combination with standard silica-coating, on the strength of bonding between zirconia (zirconium dioxide) and resin-composite cement. Silanes can form a nano-level adhesion promoting film between dissimilar materials. Zirconia is a relatively new, non-metallic biomaterial that is being used in dentistry (eg as permanent crowns and long-span bridge substructures) and in implantology (as visible attachments of metal posts in the complete replacement of one or more teeth). The advantages of zirconia that make it suitable for dental use as an alternative to metal include its inertness, greater translucence than metals, radio-opacity, and excellent biomechanical strength characteristics. In addition, it is non-soluble, chemically stable, tissue-friendly, and aesthetically white, and no adverse reactions have been reported concerning its use so far. As such, zirconia represents a promising alternative to metallic crowns, partial denture substructures, and bridge substructures, and is estimated to be used much more widely in the near future. Zirconia is attached to its substrate (eg the dentine) via a resin or resin-composite cement. However, the limiting factor underlying the performance of zirconia in terms of function and longevity seems likely to be the quality of bonding between the zirconia and resin, which is hindered exactly because of the inertness and chemical stability of zirconia. Although dentine-resin adhesive bonding has been well studied, understanding the performance of zirconia-resin bonding has been a continuous challenge because no long-term clinical experience of the longevity of zirconia has yet been reported or published. Even published in vitro test results have been limited by their lack of simulation of the wet and humid conditions in the mouth. The “standard” preparation protocol for zirconia ceramics before their use in dental restorations is sandblasting only or combined with silica-coating and followed by standard silanization, ie applying a one coat of silane coupling agent onto the silica-coated surface. To react chemically, silanes require a silica-based surface. A new concept has recently been put forward that involves the use of some not previously used silane monomers and also their blends with cross-linking silanes (both methods are studied and reported by the principal investigator). The application of a silane primer is a technique of increasing the strength of bonding between zirconia and resin by virtue of the ability of the primer to couple two unlike surfaces. Still, the characterisation of the effectiveness of silanization in increasing zirconia-resin bond strength has not been published fully, and in vitro studies would merit of novel experimental silane coupling agent primers. Finally, new approaches to achieve firm cementation continually need to be sought, in order to ensure the optimal resistance of the cementation interface to the water and humidity content of the environment under oral conditions. There is currently much activity and interest in formulating the optimal bonding protocol, as indicated in numerous in vitro study publications in respected journals in the field, such as Dental Materials, Journal of Dentistry, and Journal of Adhesive Dentistry. Zirconia surface-modification by silica-coating combined with the application of new silane primers may further enhance bonding. Accordingly, several novel experimental silane coupling agents have been developed recently (by the pricipal investigator) as primers to promote adhesion between zirconia and resin composite cement. In preliminary in vitro studies based on both wet and dry testing conditions that were performed at the principal investigator’s previous institution, these newer silanes outperformed previously used silanes as priming agents. A fuller study is proposed here to further characterise the effectiveness of these newer silanes and to obtain the complete profile of zirconia-resin bonding under simulated oral conditions to guide their possible future clinical use as zirconia primers in dentistry. Seed funding is requested for this purpose, and, importantly, to set up the appropriate in vitro research infrastructure to be able to conduct bonding strength tests under simulated oral conditions to yield data that are more relevant to clinical settings than currently used conventional tests. The modernised experimental set-up will allow the Dental Materials Science laboratory of The University of Hong Kong to have the most up-to-date and effective testing infrastructure for zirconia-substrate bonding studies that will be comparable to other world-class laboratories and will help keep Hong Kong at the forefront of dental zirconia research. |
| Project Title: | Seventh International Symposium on Silanes and Other Coupling Agents Effects of Different Silane Coupling Agents on Flexural Strength of an Experimental Filled Resin Composite |
| Investigator(s): | Matinlinna JP |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 07/2009 |
| Completion Date: | 07/2009 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Aboushelib M.N., Mirmohamadi H., Matinlinna J.P., Kukk E., Ounsi H.F. and Salameh Z., Innovations in bonding to zirconia-based materials. Part II: Focusing on chemical interactions, Dental Materials. Elsevier, 2009, 25: 989-93. |
| Heikkinen T.T., Matinlinna J.P., Vallittu P.K. and Lassila L.V.J., Dental zirconia adhesion with silicon compounds using some experimental and conventional surface conditioning methods, Silicon. 2009, 1: 199-202. |
| Heikkinen T.T., Lassila L.V.J., Matinlinna J.P. and Vallittu P.K., Effect of primers and resins on the shear bond strength of resin composite to zirconia, SRX Dentistry. 2010, 2010: 1-8. |
| Heikkinen T.T., Lassila V.J., Matinlinna J.P. and Vallittu P.K., Thermocycling Effects on Resin Bond to Silicatized and Silanized Zirconia, In: J.P. Matinlinna & K.L. Mittal, Adhesion Aspects in Dentistry. Leiden, Brill, 2009, 223-231. |
| Heikkinen T.T., Lassila L.V.J., Matinlinna J.P. and Vallittu P.K., Thermocycling effects on resin bond to silicatized and silanized zirconia, Journal of Adhesion Science and Technology. Leiden, Brill, 2009, 23: 1043-1051. |
| Matinlinna J.P. and Mittal K.L., In: J.P. Matinlinna and K.L. Mittal, Adhesion Aspects in Dentistry. Leiden, The Netherlands, VSP Brill, 2009. |
| Matinlinna J.P., Amalgam Issues. Part I (in Finnish : Asiaa amalgaamista. Osa I), Hammasteknikko. 2010, 2: 20-23. |
| Matinlinna J.P., Dental Nurse is Smiling in Hong Kong (In Finnish: Hammashoitaja hymyilee Hong Kongissa), Suun Terveydeksi. 2009, 3: 12-13. |
| Matinlinna J.P., Dental Technician Education in Hong Kong (In Finnish : Hammasteknikkokoulutus Hong Kongissa), Hammasteknikko. 2010, 1: 8-9. |
| Matinlinna J.P., Editorial Board, Dental Materials. 2010. |
| Matinlinna J.P., Editorial Board, Journal of Adhesion Science and Technology (JAST). 2010. |
| Matinlinna J.P., Editorial Board, Open Dentistry Journal. 2010. |
| Matinlinna J.P., Editorial Board, Silicon. 2010. |
| Matinlinna J.P., Lassila L.V.J. and Vallittu P.K., Experimental novel silane system in adhesion promotion between dental resin and pretreated titanium, Silicon. 2009, 1: 249-254. |
| Matinlinna J.P., Guest Editorial, Journal of Adhesion Science and Technology. 2009, 23: 933-934. |
| Matinlinna J.P. and Mittal K.L., In: J.P. Matinlinna and K.L. Mittal, Journal of Adhesion Science and Technology, Special issue on "Adhesion Aspects in Dentistry". Leiden, Brill, 2009, 23: 931-1214. |
| Puska M., Lassila L., Vallittu P.K., Seppälä J. and Matinlinna J.P., Evaluation of bis-GMA/MMA Resin Adhesion to Silica-Coated and Silanized Titanium, In: J.P. Matinlinna and K.L. Mittal, Adhesion Aspects in Dentistry. Leiden, Brill, 2009, 147-162. |
| Puska M., Lassila L., Vallittu P.K., Seppälä J. and Matinlinna J.P., Evaluation of bis-GMA/MMA resin adhesion to silica-coated and silanized titanium, Journal of Adhesion Science and Technology. Leiden, Brill, 2009, 23: 991-1006. |
| Researcher : McGrath CPJ |
| Project Title: | 81st General Session and Exhibition of the International Association for Dental Research Do Parents Know Their Children's Oral Health? |
| Investigator(s): | McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2003 |
| Abstract: |
| N/A |
| Project Title: | 51st Annual ORCA (European Organisation for Caries Research) Congress Agreement between Photographic and Clinical Assessments of Development Defects of Enamel among Anterior Teeth |
| Investigator(s): | McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2004 |
| Abstract: |
| N/A |
| Project Title: | British Society for the Study of Community Dentistry (BASCD) AGM and Scientific Meeting 2005 A Comparison of Imputation and Deletion Techniques for Handling Missing Data |
| Investigator(s): | McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 03/2005 |
| Abstract: |
| N/A |
| Project Title: | A randomized clinical trial of the treatment of obstructive sleep apnoea using oral appliances |
| Investigator(s): | McGrath CPJ, Hagg EUO, Ip MSM |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 12/2009 |
| Abstract: |
| 1) To assess changes in sleep apnoea patients’ quality of life following oral appliance therapy and compare changes in patients’ quality of life following the use of two types of oral appliances (monobloc versus twinblock); 2) To determine changes in the physiological aspects of sleep apnoea following oral appliance therapy and compare changes in physiological aspects of sleep apnoea following the use of monobloc versus twinblock appliances; 3) To determine obstructive sleep apnoea patients’ compliance and satisfaction with oral appliances and compare patient compliance and satisfaction following the use of monobloc versus twinblock appliances. |
| Project Title: | A spatial analysis of the “Children of 1997” birth cohort’s oral health – is it a ‘fat’ issue? |
| Investigator(s): | McGrath CPJ, Wong HM, King NM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2010 |
| Abstract: |
| Objectives: This study aims to determine the oral health status of the “children of 1997” birth cohort and to provide a spatial analysis (geographic mapping) of their oral health in relation to socio-economic status (SES) and adiposity. Methods: A stratified random sample of 600 12-year-old children will have an oral health examination according to the World Health Organization’s methods and criteria. Anthropometric assessments of adiposity will also be conducted by a trained examiner. SES will be assessed at household and neighbourhood level. The relationship between oral health status, adiposity and SES will be analyzed using univariate, bivariate and regression analysis (including multilevel analysis). A spatial analysis using geographic information systems (GIS) will be conducting to provide a geographical mapping of oral health with respect to SES and adiposity. The findings of this study will provide for the first time in Hong Kong (i) geographical mapping of oral health data; (ii) information on the relationship between oral health and SES at both household and neighborhood levels; (iii) information on the relationship between oral health and adiposity. Such information will form the basis of preliminary work of a GRF grant on the trajectory of oral health among the ‘Children of 1997’ birth cohort. Background Information Maps have been used as a public health tool for over a century. The research of Dr. John Snow on the cause of the cholera epidemic in London in the mid-1800s is one of the earliest published examples (Lang 2000). In 1854, an outbreak of cholera occurred in the Soho district, Dr. John Snow created maps manually to show the pattern of cholera victims in the neighborhood of one well constitute a classic use of geographic information to draw epidemiological conclusions. With the development of computer technology, geographic information systems (GIS) technology has experienced a dramatic progress from 1960s (Parker 2008). Now, GIS are computer systems using numerous software applications capable of assembling, storing, manipulating, and displaying geographically referenced information, i.e. data identified according to their locations. With GIS, researches can link information to location data, such as people to addresses, allowing you to see relationships or patterns intuitively that are not easy to see with traditional charts, graphs, or spreadsheets (Steinberg 2006). Therefore, a new science named geographical epidemiology has come about via the collection and analysis of spatial patterns of disease appearance, taking into consideration the social, economic, ecological and demographic prerequisites in space and time. Geographical epidemiology covers almost every aspect of public health but unfortunately it has not been employed to any great extent in dentistry. By mapping demographic factors in relation to disease conditions it can predict target populations for a disease to support programs to prevent its spread (Lang 2000). For example, studies on the relationship between distribution of environmental factors (air, water pollution) and the incidence/outbreak of a disease (cholera), socio-economic indicators and the distribution of a disease (TB, cancer) (Chan-yeung et al 2005), inequalities of the neighborhood health facilities and health status (Berke et al 2007). To date, in public health dentistry few studies using spatial analyses as means of data analysis. Their focus has been on the relationship between inequalities of the neighborhood health facilities and/or human resources (distribution of dentist) and treatment needs or oral health status in the West (McCormick et al 2008; Borrell et al 2006; Krause et al 2005). This study aims to determine whether the household and neighbourhood SES indicators predict the distribution of dental caries in Hong Kong using GIS technology; accounting for adiposity. The findings will demonstrate the inner relationship between dental caries status and body composition. In addition, it will specify the best adiposity index to predict dental caries status. It will also provide strong evidence to explain whether SES factors affect dental caries status in Hong Kong. These findings have potential to place GIS technology at the very centre of dental public health care planning. Reference Berke EM, Koepsell TD, Moudon AV, Hoskins RE, Larson EB. Association of the built environment with physical activity and obesity in older persons. Am J Public Health 2007; 97: 486-492. Borrell LN, Northridge ME, Miller DB, Golembeski CA, Spielman SE, Sclar ED, Lamster IB. Oral health and health care for older adults: a spatial approach for addressing disparities and planning services. Spec Care Dentist 2006; 26: 252-256. Chan-yeung M, Yeh AG, Tam CM, Kam KM, Leung CC, Yew WW, Lam CW. Socio-demographic and geographic indicators and distribution of tuberculosis in Hong Kong: a spatial analysis. Int J Tuberc Lung Dis 2005; 9: 1320-1326. Krause D, Frate DA, May WL. Demographics and distribution of dentists in Mississippi: a dental work force study. J Am Dent Assoc 2005; 136: 668-677. Lang L. GIS for health organizations. ESRI Press: Redlands, Calif. 2000. McCormick RJ, Smith R, Edwards D, White D, Langford J. The distribution of general dental practitioners with NHS contract numbers in relation to the distance of their practices from the seven dental undergraduate teaching hospitals in England outside London. Community Dent Health 2008; 25: 201-204. Parker RN. GIS and spatial analysis for the social sciences : coding, mapping and modeling. Routledge: New York 2008. Steinberg SJ. GIS : geographic information systems for the social sciences : investigating space and place. SAGE Publications: Thousand Oaks, Calif. 2006. |
| List of Research Outputs |
| Bootvong K., Liu Z., McGrath C.P.J., Hagg E.U.O., Wong R.W.K., Bendeus S.A.M. and Yeung S., Virtual model analysis as an alternative approach to plaster model analysis: reliability and validity, The European Journal of Orthodontics. 2010, 32: 589-595. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience - Journal Based Learning and Fundamentals of Dental Research – a pilot programme, the 6th International Conference on PBL in Dentistry. Hong Kong, 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience-journal based learning and fundamentals of dental research-a pilot programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.26. |
| Bridges S.M., Botelho M.G., Dyson J.E., McGrath C.P.J., Tsang P.C.S. and Yiu C.K.Y., Blended learning and interactive pedagogies: Supporting problem-based learning in dental education, CITE Research Symposium 2010. 2010. |
| Bridges S.M., McGrath C.P.J., Wong H.M. and Au T.K.F., Exploring The Dimensions Of Oral Health Literacy. , In: Bridges, S. M., McGrath, C. P. J., Wong, H. M., & Au, T. K. , The 9th World Congress On Preventive Dentistry, Phuket, Thailand.. 2009. |
| Bridges S.M., Corbet E.F., Samaranayake L.P., Dyson J.E., Leung W.K. and McGrath C.P.J., Improving programme evaluations: The effect of curriculum review, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 37. |
| Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., Multilingual clinical interactions in Dentistry: A microanalysis, 11th International Pragmatics Conference. Australia, 2009. |
| Bridges S.M., McGrath C.P.J., Yiu C.K.Y. and Cheng S.S., Reassuring' during clinical examinations: Novice and expert talk in dentistry, Journal of Asian Pacific Communication. John Benjamins, 2010, 20:2: 185-206. |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Choi W.W.S., Lee S..., McGrath C.P.J. and Samman N., Change in quality of life after combined orthodontic-surgical treatment of dentofacial deformities, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2010, 109 (1): 46-51. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Du R.Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health- and oral health-related quality of life among preschool children with cerebral palsy, Quality of Life Research. 2010, 19: 1367-1371. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Du RY, McGrath C, Yiu CKY, King NM. Health-related and oral health-related quality of life among preschool children with cerebral palsy. Best Poster Presentation , Conjoint scientific sessions – Faculty of Dentistry, the University of Hong –Peking University School of Stomatology, 16-17 November 2009. 2009. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health and oral health-related quality of life among preschool children with Cerebral Palsy, 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health-related and oral health-related quality of life among preschool children with cerebral palsy. , Conjoint Scientific Session. The University of Hong Kong-Peking University School of Stomatology, 16-17 November, 2009. p.32. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Oral health in the primary dentition of children with cerebral palsy. , 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Gao X., McGrath C.P.J. and Lin H.C., Oral Health Status of Rural-Urban Migrant Children in South China, International Dental Association for Dental Research 88th General Session. 2010. |
| Hagg E.U.O., Khanoengnit B., Liu Z., McGrath C.P.J. and Wong R.W.K., Virtual models as an alternative approach to plaster model assessment of tooth dimension, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p106 (SP284). |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and Responsiveness of ChineseECOHIS to Dental Treatment, 1st Hong Kong International Dental Expo and Symposium. 2010. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and responsiveness of the Chinese version of the Early Childhood Oral Health Impact Scale (ECOHIS) to dental treatment under general anaesthesia. , Conjoint Scientific Session 2009. The University of Hong Kong-Peking University School of Stomatology, 16-17 Kong November 2009. p.32. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Translation and validation of a Chinese language version of the Early Childhood Oral Health Impact Scale (ECOHIS), International Journal of Paediatric Dentistry. 2009, 19: 399-405. |
| Lu H.X., Wong M.C.M., Lo E.C.M. and McGrath C.P.J., Factors associated with oral health from childhood to early adulthood, Journal of Dental Research. 2009, 88 (Spec Iss B): 313 (PAPF/APR). |
| McGrath C.P.J., Zhang W. and Lo E.C.M., A review of the effectiveness of oral health promotion activities among elderly people, Gerodontology. 2009, 26: 85-96. |
| McGrath C.P.J., Invited lectures, "The value and use of quality of life measures in orthodontics", "Selecting appropriate quality of life measures for orthodontics", and "Interpreting qualify of life data in clinical practice and research", Finnish Orthodontic Society, Helsinki, Finland, March 26-27. 2010. |
| McGrath C.P.J., Cheung K., Hong P.Y., Hui B.S.Y., Leung C.Y., Leung K.W., Tang C.F., Tse Y.K., Wang Z. and Wong H.C., Post-traumatic stress and oral health among asylum seekers in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 169: 1-38. |
| Verayannont P., Hagg E.U.O., Wong R.W.K., McGrath C.P.J. and Yeung S., Maxillary growth during puberty determined by the implant method compared with cephalometry, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p70 (SP119). |
| Wong M.C.M., Lu H.X., Lo E.C.M. and McGrath C.P.J., Oral health status of young adults in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 308 (PAPF/APR). |
| Wu A., McGrath C.P.J., Wong R.W.K., Wiechmann D. and Rabie A.B.M., Pain and daily oral impact and satisfaction of patients treated with labial and lingual fixed appliances, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p18 (OP033). |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath C.P.J., Comprehensive Cephalometric Analyses of 10 to 14-Year Old Southern Chinese, The Open Anthropology Journal 2010. 2010, 3: 85-95. |
| Zhang W., McGrath C.P.J. and Lo E.C.M., A comparison of root caries diagnosis based on visual-tactile criteria and DIAGNOdent in vivo, Journal of Dentistry. 2009, 37: 509-513. |
| Zhu H., McMillan A.S. and McGrath C.P.J., Oral health-related quality of life after stroke, Journal of Dental Research. 2009, 88 (Spec Iss B): 610 (PAPF/APR). |
| Researcher : McMillan AS |
| Project Title: | Orofacial Pain and its Functional and Psychosocial Impact |
| Investigator(s): | McMillan AS, Wong MCM, Lam CLK, Luo Y |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2005 |
| Abstract: |
| This is a cross-disciplinary study involving the Faculties of Dentistry and Medicine that is directly related to the major research theme of Healthy Ageing. Hypotheses: o A substantial number of community-dwelling adults in Hong Kong suffer from orofacial pain (OFP) and the majority of them do not seek professional help for their condition o OFP is an under-treated condition that causes significant disability and has a serious adverse effects on people's well-being and quality of life. Purpose: To investigate a) the prevalence of OFP and its diagnostic sub-groups in community dwelling adult people in Hong Kong, b) the adverse impact of OFP on functional and psychosocial wellbeing, and c) barriers to treatment seeking for OFP symptoms. Objectives: o To determine the frequency, severity and pattern of OFP according to diagnostic sub-groups using validated patient-centred measures and clinical assessment o To describe the treatment seeking behaviour associated with OFP and factors associated with such behaviour o To assess the impact of OFP on health-related QoL using generic and condition specific health status measures o To correlate patient descriptions of OFP with clinical diagnoses Pain is closely associated with poor health. The negative effect of pain on health outcomes such as function and quality of life (QoL) among patient populations is well documented (Ahmedzai, 1995). However, little is known about the impact of pain on subjective health and well-being in community dwelling populations (Reyes-Gibby et al, 2002). Although pain is a common symptom of dental and oral disease and can have a significant impact, there is a shortage of systematic data on the prevalence, severity and consequences of orofacial pain (OFP) in general, non-clinical populations (Nikias, 1985). In a community based study in Toronto, Locker & Grushka (1987) noted that 53% of participants had experienced some pain or discomfort in the orofacial region in the previous four weeks. The majority of pain reports were tooth or gum related whereas pain in the jaws, face and oral mucosa were less common. Epidemiological studies in the USA, Canada and the United Kingdom have shown that the prevalence of oro-facial pain symptoms in adult populations ranges from 14 to 40% (Lipton et al, 1993; Locker & Grushka, 1987; Macfarlane et al 2002). Gender and age-related differences in oro-facial pain prevalence have also been noted with women and younger adults at apparently greater risk of pain symptoms, whereas older people are more likely to suffer more severe pain (Macfarlane et al, 2002; Von Korff et al, 1988). Pain may have considerable impact on people's ability to perform effectively at work, home and school. Nonetheless, not everyone seeks professional help for pain with some symptoms being ignored while others may prompt action (Macfarlane et al, 2003). It is, therefore, important to discern factors that may distinguish individuals who seek professional care from those who do not. In a population-based study of OFP in the UK, Macfarlane et al (2003) noted that less than half of their study population with OFP sought professional advice. The majority of factors associated with seeking treatment appeared to be various characteristics of pain although the time course of these factors was unclear. Locker & Grushka (1987) observed that while more than 50% of their OFP study group described pain severity as moderately severe or severe, only 40 % of those reporting pain sought advice from a doctor of dentist. Ethnic differences in the perception of and means of managing pain account, at least in part, for observed differences in treatment seeking behaviour for orofacial pain conditions (Hastie et al, 2005). Socioeconomic status is also an important factor related to treatment seeking with oral disease generally more common among those least able to access health care and leads to needless pain and suffering (Riley et al, 2005). There is presently very little information on the prevalence and impact of oro-facial pain in Southern Chinese people in Hong Kong. Most oro-facial pain prevalence studies have focused on predominantly Caucasian populations. However, a recent study in Korean elders indicated that the prevalence was higher in this ethnic group and significant disability was associated with the condition (Chung et al, 2004). In a previous population-based survey, we have shown that self-reported pain symptoms associated with temporomandibular disorders are relatively common in the Hong Kong Chinese population (Pow et al, 2001). However, the characteristics of the pain differed from other ethnic groups, no gender bias in pain symptoms was observed and treatment seeking was much lower than in Caucasian counterparts. One of the problems associated with the available epidemiological data on prevalence of OFP is that it has been collected solely by means of questionnaires with patient self-reports not being confirmed by clinical assessment (Macfarlane et al, 2004). There is, therefore, the risk that patient described pain symptoms are misinterpreted and pain symptoms due to multiple conditions may go unrecognized. Definitive diagnoses can only be obtained by clinical assessment. References: Ahmedzai S. Recent clinical trials of pain control: impact on quality of life. Eur J Cancer 1995;31A(Suppl):S2-7 Chung JW, Kim JH, Kim HD, Kho HS, Kim YK, Chung SC. Chronic orofacial pain among Korean elders: prevalence, and impact using the graded chronic pain scale. Pain 2004;112:164-170 Hastie BA, Riley III JL, Fillingim RB. Ethnic differences and responses to pain in healthy young adults. Pain Medicine 2005;6:61-71 Lipton JA, Ship JA, Larach-Robinson D. Estimated prevalence and distribution of reported orofacial pain in the United Sates. J Am Dent Assoc 1993;124:115-121 Locker D, Grushka M. Prevalence of oral and facial pain and discomfort: preliminary results from a mail survey. Community Dent Oral Epidemiol 1987;15:169-172 Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV. Factors associated with health care seeking behaviour for orofacial pain in the general population. Community Dent Health 2003;20:20-26 Macfarlane TV, Blinkhorn AS, Craven R et al. Can one predict the likely specific orofacial pain syndrome from a self-completed questionnaire? Pain 2004;111:270-277 Nikias M. Oral disease and the quality of life. Am J Public Health 1985;75:11-12 Reyes-Gibby C, Aday L, Cleeland C. Impact of pain on self-related health in community-dwelling older adults. Pain 2002;95:75-82 Riley III JL, Gilbert GH. Orofacial pain symptoms: an interaction between age and sex. Pain 2001;90:245-256 Von Korff M, Ormel J, Keefe FJ, Dworkin SF. Grading the severity of chronic pain complaints. Pain 1988;32:173-183 |
| Project Title: | Predictors of outcome of orofacial pain: A community-based longitudinal study |
| Investigator(s): | McMillan AS, Wong MCM, Lam CLK, Luo Y, Zheng J |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2006 |
| Completion Date: | 10/2009 |
| Abstract: |
| This is a cross-disciplinary study involving the Faculties of Dentistry and Medicine that is directly related to the major research theme of Healthy Ageing. Purpose: To investigate a) the pain characteristics in community-dwelling adult Hong Kong Chinese people with OFP, b) to follow-up OFP sufferers over a 6-month period and identify presenting factors and behaviours that predict poor outcome. Null hypotheses: - Orofacial pain does not tend to persist - There are no factors that can predict the persistence of OFP Objectives: o To describe the outcome of OFP after a period of 6-months o To identify baseline factors that predict poor outcome in adults with OFP Orofacial pain (OFP) may be defined as pain related to the face and/or mouth and involves both hard and soft tissues in these anatomical regions. Epidemiological studies in the USA, Canada and the United Kingdom have shown that the prevalence of OFP symptoms in adult populations ranges from 14 to 40%.1-3 In a population-based survey of OFP symptoms in adult Chinese people in Hong Kong, the one-month period prevalence of OFP (42%) was found to be consistent with estimates in western countries.4 Pain is closely associated with poor health and may considerably impair people's ability to perform daily living activities. Despite this, not all pain sufferers seek professional help with some symptoms being ignored whilst others prompt attention. (2,3,5,6). In studies involving western cohorts 44-46% with OFP sought professional help (2,5,6). However, only 20% of Hong Kong Chinese adults with OFP symptoms sought professional treatment.4 Thus, it is important to explore the factors and barriers relating to professional treatment seeking behaviour in order to understand the disparity between Chinese adults and their western counterparts. MacFarlane et al5 noted that pain characteristics appeared to prompt treatment seeking although the time course of these factors was unclear. The decision to seek treatment may be related to the consequences of the OFP as experienced by the individual and also affected by culturally-mediated responses to the pain experience and its consequences (7). A key characteristic of OFP is that it tends to persist (8). In a longitudinal study in the United Kingdom, over half those with OFP at baseline reported pain four years later (9). Persistent pain was associated with females, increasing age, psychological distress, widespread body pain and taking pain medication at baseline. In a Swedish cohort, the follow-up of 109 patients with OFP revealed that after 4 to 9 years only 27% had resolution of the pain, with pain medication usage at baseline a strong predictor of persistent OFP (10). The consequences of OFP have an impact on various aspects of daily life and include work loss, sleep disturbance, need for more rest, avoidance of social contacts, and difficulty eating and speaking (2,11). The magnitude of the impact appears to be related to the pain characteristics and socio-demographics of the respondents (2,3,11). Pain-related sleep disturbance is also a key determinant of seeking professional treatment (6). There is scant information available on the characteristics of OFP in southern Chinese people, its potential to persist and the functional and psychosocial impact. In a population-based survey of symptoms of temporomandibular disorders in Hong Kong Chinese people, jaw pain, restricted jaw opening and jaw clicking were the most common presenting symptoms and often associated with significant morbidity (12). In contradistinction to western studies, there were no gender-related differences in symptom reporting or treatment seeking (12). In a recent study of chronic OFP in elderly Koreans the prevalence appeared to be higher compared with Caucasian populations.13Adverse impacts were common with around 30% of those with joint pain, toothache and burning mouth symptoms having significant associated disability and impairment of daily living activities (13). The ideal approach to the investigation of OFP in the community is by interview using validated questionnaires and clinical assessment. However, most studies where patient-based and clinical data are available involve a bias sample of people seeking treatment. In epidemiological studies of OFP, the most common means of obtaining information is by questionnaire, by mail or telephone survey, without clinical examination of the participants. Even with contemporary questionnaire approaches, without clinical assessment, there is always the issue of data reliability. References 1. Lipton JA, Ship JA, Larach-Robinson D. Estimated prevalence and distribution of reported orofacial pain in the United States. J Am Dent Assoc 1993;124:115-21. 2. Locker D, Grushka M. Prevalence of oral and facial pain and discomfort: preliminary results of a mail survey. Community Dent Oral Epidemiol 1987a;15:169-72. 3. Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV (2002). Oro-facial pain in the community: prevalence and associated impact. Community Dent Oral Epidemiol 30(1):52-60. 4. McMillan AS, Wong MCM, Zheng J, Lam CLK. Prevalence of orofacial pain and treatment seeking in Hong Kong Chinese. J Orofac Pain (in press) 5. Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV. Factors associated with health care seeking behaviour for orofacial pain in the general population. Community Dent Health 2003;20:20-26. 6. Locker D. The symptom iceberg in dentistry. Treatment-seeking in relation to oral and facial pain. J Can Dent Assoc 1988:54:271-4. 7. Hastie BA, Riley JL, Fillingim RB. Ethnic differences and responses to pain in healthy young adults. Pain Med 2005;6:61-71. 8.Von Korff M, Ormel J, Keefe F, Dworkin S (1992). Grading the severity of chronic pain. Pain 50(2):133-149. 9.Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV. Predictors of outcome for orofacial pain in the general population: a four-year follow-up study. 2004 J Dent Res ;83:712-7. 10. Wolf E, Nilner M, Petersson A, Petersson K (2002). Long-term follow-up by means of a questionnaire of 109 patients with long-lasting orofacial pain. Swed Dent J. 26(3):125-34. 11. Locker D, Grushka M. The impact of dental and facial pain. J Dent Res 1987;66:1414-7. 12. Pow EHN, Leung KCM, McMillan AS. Prevalence of symptoms associated with temporomandibular disorders in Hong Kong Chinese. J Orofac Pain 2001;15:228-34. 13. Chung JW, Kim JH, Kim HD, Kho HS, Kim YK, Chung SC. Chronic orofacial pain among Korean elders: prevalence, and impact using the graded chronic pain scale. Pain 2004;112:164-70. |
| Project Title: | A randomized clinical trial on the effect of Chlorhexine mouth rinse and assisted tooth brushing on the health condition and qulaity of life of elderly stroke patients |
| Investigator(s): | McMillan AS, McGrath CPJ, Li LSW, Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2007 |
| Abstract: |
| The main purpose of this oral health intervention project is to compare the effect of providing 1) professional oral hygiene instruction alone versus 2) professional oral hygiene instruction plus adjunctive Chlorhexidine mouth rinse versus 3) professional oral hygiene instruction plus adjunctive assisted tooth brushing and Chlorhexidine mouth rinse, on the oral health condition, the general health condition and health-related quality of life (HRQoL) of patients with stroke who are receiving hospital-based rehabilitation. An additional purpose is to assess potential longer term oral intervention-related health and HRQoL outcomes. Null hypotheses: 1. There is no difference in a) the amount of dental plaque, gingival bleeding and oral carriage of yeasts and coliforms, b) morbidity or mortality levels, and c) the change in HRQoL between stroke patients who have received professional oral hygiene instruction alone compared with professional oral hygiene instruction plus adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. 2. There is no difference in the change in health condition and HRQoL among the three treatment groups, 6-months after completion of the oral intervention. Objectives: To describe and compare prospectively over a 4-week period during hospital-based rehabilitation, 1a. Changes in the amount of dental plaque, gingival bleeding and oral carriage of yeasts and coliforms in stroke patients after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. b. Changes in morbidity and mortality levels in stroke patients after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. c. Changes in HRQoL in stroke patients after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. 2. To describe and compare changes in health condition and HRQoL in stroke patients 6-months after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. Stroke is one of the commonest causes of brain injury in the elderly and leads to significant disability and mortality (Counsell et al, 1995). Depending on the severity and type, stroke leaves an individual with residual impairment of physical, psychological and social functions. Limb paralysis and reduced manual dexterity are common physical features (Scully & Cawson, 1998). Up to 85% of stroke patients have an initial deficit in the arm and hand and problems remain in 55-75% of patients three to six months later (Lai et al, 2002). Oro-facial manifestations of stroke include unilateral facial palsy that mainly affects the lower face, tongue and soft palate. Stroke-related oral motor impairment is very disabling as speech, mastication and swallowing problems are common and such key behaviours do not always fully recover (Smithard, 2001). Impaired manual dexterity affects the stroke survivors’ ability to clean their teeth and dentures and maintain a healthy oral condition and together with impaired oral function-related inability to clear the mouth of food debris result in halitosis, caries and an increased risk of oral microbial infections notably Candida and coliform species (Rose et al, 2002). Tooth brushing is the mainstay of good oral hygiene and health. However, after stroke, conventional tooth brushing is difficult and often ineffective. Battery-operated tooth brushes are more effective than conventional tooth brushes for disabled patients (Kamen, 1997). Even then, good oral hygiene may be difficult to attain without assistance from a care-giver. A combination of mechanical and chemical interventions have proven efficacy in the prevention of periodontal diseases (Santos et al, 2004). Chlorhexidine, a bisguanide, is considered to be the most effective chemical agent in dental plaque control and is used routinely in mouth rinse form as an adjunct in the treatment of periodontal diseases (Van Strydonck et al, 2005). It displays low toxicity and microbial resistance and supra-infection do not appear to occur, and its persistence prolongs anti-bacterial action (Paraskevas, 2005). Measurement of health related quality of life (HRQoL) in stroke sufferers provides information on recovery, identifies predictors of patient outcome and assists in the evaluation of interventions (Pickard et al, 1999). An effective approach to the measurement of quality of life is a combination of generic and specific questionnaires (Allen, 2003). The Medical Outcomes Short-Form Health Survey questionnaire (SF-36) is a generic measure that has been validated for use in stroke patients (Dorman et al, 1999). The SF-12 also exhibits an acceptable level of responsiveness to change in stroke patients (Muller-Nordhorn et al, 2005). The Oral Health Impact Profile (OHIP-49) and its short form version are sophisticated oral specific instruments that measure the impact of oral conditions on HRQoL (Allen, 2003). References: Allen PF. Assessment of oral health related quality of life. Health Qual Life Outcomes. 2003 Sep 8;1:40 Counsell C, Wardlow C, Sandercock P et al. The Cochrane Collaboration Stroke Review Group. Meeting the need of systematic reviews in stroke care. Stroke 1995;26:498-502 Dorman PJ, Dennis M, Sandercock P. How do scores on the EuroQol relate to scores on the SF-36 after stroke? Stroke 1999;30:2146-2151 Kamen S. Oral health care for the stroke survivor. J California Dent Assoc 1997;25:297-303 Lai SM, Studenski S, Duncan PW, Perera S. Persisting consequences of stroke measured by the stroke impact scale. Stroke 2002;33:1840-1844 Muller-Nordhorn J, Nolte CH, Rossnagel K et al. The use of the 12-item short-form health status instrument in a longitudinal study of patients with stroke and transient ischaemic attack. Neuroepidemiology 2005;24:196-202 Parakevas S. randomized controlled clinical trials on agents used for chemical plaque control. Int J Dent Hygiene 2005;3:162-178 Pickard AS, Johnson JA, Penn A et al. Replicability of SF-36 summary scores by the SF-12 in stroke patients. Stroke 1999;30:1213-1217 Rose LF, Mealey B, Minsk L, Cohen W. Oral care for patients with cardiovascular disease and stroke. JADA 2002;133:375-345 Santos S, Herrera D, Lopez E, O’Connor A et al. A randomized clinical trial on the short-term clinical and microbiological effects of the adjunctive use of 0.05% chlorexidine mouth rinse for patients in supportive periodontal care. J Clin Periodontol 2005;31:45-51 Scully C, Cawson RA. Medical Problems in Dentistry. Wright, UK, 1998 Smithard DG. Swallowing and stroke. Neurological affects and recovery. Cerebrovasc Dis 2002;14:1-8 Van Strydonk DAC, Timmerman MF, van der Velden U et al. Plaque inhibition of two commercially available chlorhexidine mouthrinses. J Clin Periodontol 2005;32:30530-9 |
| Project Title: | 86th General Session & Exhibition of the International Association for Dental Research (IADR) Treatment seeking behaviour in Southern Chinese people with orofacial pain |
| Investigator(s): | McMillan AS |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 07/2008 |
| Abstract: |
| N/A |
| List of Research Outputs |
| McMillan A.S., Wong M.C.M., Zheng J., Luo Y. and Lam C.L.K., Widespread pain symptoms and psychological distress in southern Chinese with orofacial pain, Journal of Oral Rehabilitation. 2010, 37: 2-10. |
| Perera R.A.P.M., Tsang P.C.S., McMillan A.S., Miller C.S., Lee M.P., Li P. and Samaranayake L.P., Oral chemokine interactome unfolds potential anti-HIV mucosal microbicide targets, 23rd IADR Annual Scientific Meeting . 2009. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., A comparison of laboratory extraction techniques for the detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients., Oral Oncology. 2009, 3: 200. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., Detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients: a comparison of two laboratory extraction techniques, Conjoint Scientific Sessions Faculty of Dentistry, The University of Hong Kong - Peking University School of Stomatology. 2009. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Zheng J., McMillan A.S., Wong M.C.M., Luo Y. and Lam C.L.K., Investigation of factors associated with treatment-seeking in southern Chinese with orofacial pain, Journal of Orofacial Pain. 2010, 24: 181-188. |
| Zhu H., McMillan A.S. and McGrath C.P.J., Oral health-related quality of life after stroke, Journal of Dental Research. 2009, 88 (Spec Iss B): 610 (PAPF/APR). |
| Researcher : Mcgrath CPJ |
| Project Title: | 81st General Session and Exhibition of the International Association for Dental Research Do Parents Know Their Children's Oral Health? |
| Investigator(s): | McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2003 |
| Abstract: |
| N/A |
| Project Title: | 51st Annual ORCA (European Organisation for Caries Research) Congress Agreement between Photographic and Clinical Assessments of Development Defects of Enamel among Anterior Teeth |
| Investigator(s): | McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2004 |
| Abstract: |
| N/A |
| Project Title: | British Society for the Study of Community Dentistry (BASCD) AGM and Scientific Meeting 2005 A Comparison of Imputation and Deletion Techniques for Handling Missing Data |
| Investigator(s): | McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 03/2005 |
| Abstract: |
| N/A |
| Project Title: | A randomized clinical trial of the treatment of obstructive sleep apnoea using oral appliances |
| Investigator(s): | McGrath CPJ, Hagg EUO, Ip MSM |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 12/2009 |
| Abstract: |
| 1) To assess changes in sleep apnoea patients’ quality of life following oral appliance therapy and compare changes in patients’ quality of life following the use of two types of oral appliances (monobloc versus twinblock); 2) To determine changes in the physiological aspects of sleep apnoea following oral appliance therapy and compare changes in physiological aspects of sleep apnoea following the use of monobloc versus twinblock appliances; 3) To determine obstructive sleep apnoea patients’ compliance and satisfaction with oral appliances and compare patient compliance and satisfaction following the use of monobloc versus twinblock appliances. |
| Project Title: | A spatial analysis of the “Children of 1997” birth cohort’s oral health – is it a ‘fat’ issue? |
| Investigator(s): | McGrath CPJ, Wong HM, King NM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2010 |
| Abstract: |
| Objectives: This study aims to determine the oral health status of the “children of 1997” birth cohort and to provide a spatial analysis (geographic mapping) of their oral health in relation to socio-economic status (SES) and adiposity. Methods: A stratified random sample of 600 12-year-old children will have an oral health examination according to the World Health Organization’s methods and criteria. Anthropometric assessments of adiposity will also be conducted by a trained examiner. SES will be assessed at household and neighbourhood level. The relationship between oral health status, adiposity and SES will be analyzed using univariate, bivariate and regression analysis (including multilevel analysis). A spatial analysis using geographic information systems (GIS) will be conducting to provide a geographical mapping of oral health with respect to SES and adiposity. The findings of this study will provide for the first time in Hong Kong (i) geographical mapping of oral health data; (ii) information on the relationship between oral health and SES at both household and neighborhood levels; (iii) information on the relationship between oral health and adiposity. Such information will form the basis of preliminary work of a GRF grant on the trajectory of oral health among the ‘Children of 1997’ birth cohort. Background Information Maps have been used as a public health tool for over a century. The research of Dr. John Snow on the cause of the cholera epidemic in London in the mid-1800s is one of the earliest published examples (Lang 2000). In 1854, an outbreak of cholera occurred in the Soho district, Dr. John Snow created maps manually to show the pattern of cholera victims in the neighborhood of one well constitute a classic use of geographic information to draw epidemiological conclusions. With the development of computer technology, geographic information systems (GIS) technology has experienced a dramatic progress from 1960s (Parker 2008). Now, GIS are computer systems using numerous software applications capable of assembling, storing, manipulating, and displaying geographically referenced information, i.e. data identified according to their locations. With GIS, researches can link information to location data, such as people to addresses, allowing you to see relationships or patterns intuitively that are not easy to see with traditional charts, graphs, or spreadsheets (Steinberg 2006). Therefore, a new science named geographical epidemiology has come about via the collection and analysis of spatial patterns of disease appearance, taking into consideration the social, economic, ecological and demographic prerequisites in space and time. Geographical epidemiology covers almost every aspect of public health but unfortunately it has not been employed to any great extent in dentistry. By mapping demographic factors in relation to disease conditions it can predict target populations for a disease to support programs to prevent its spread (Lang 2000). For example, studies on the relationship between distribution of environmental factors (air, water pollution) and the incidence/outbreak of a disease (cholera), socio-economic indicators and the distribution of a disease (TB, cancer) (Chan-yeung et al 2005), inequalities of the neighborhood health facilities and health status (Berke et al 2007). To date, in public health dentistry few studies using spatial analyses as means of data analysis. Their focus has been on the relationship between inequalities of the neighborhood health facilities and/or human resources (distribution of dentist) and treatment needs or oral health status in the West (McCormick et al 2008; Borrell et al 2006; Krause et al 2005). This study aims to determine whether the household and neighbourhood SES indicators predict the distribution of dental caries in Hong Kong using GIS technology; accounting for adiposity. The findings will demonstrate the inner relationship between dental caries status and body composition. In addition, it will specify the best adiposity index to predict dental caries status. It will also provide strong evidence to explain whether SES factors affect dental caries status in Hong Kong. These findings have potential to place GIS technology at the very centre of dental public health care planning. Reference Berke EM, Koepsell TD, Moudon AV, Hoskins RE, Larson EB. Association of the built environment with physical activity and obesity in older persons. Am J Public Health 2007; 97: 486-492. Borrell LN, Northridge ME, Miller DB, Golembeski CA, Spielman SE, Sclar ED, Lamster IB. Oral health and health care for older adults: a spatial approach for addressing disparities and planning services. Spec Care Dentist 2006; 26: 252-256. Chan-yeung M, Yeh AG, Tam CM, Kam KM, Leung CC, Yew WW, Lam CW. Socio-demographic and geographic indicators and distribution of tuberculosis in Hong Kong: a spatial analysis. Int J Tuberc Lung Dis 2005; 9: 1320-1326. Krause D, Frate DA, May WL. Demographics and distribution of dentists in Mississippi: a dental work force study. J Am Dent Assoc 2005; 136: 668-677. Lang L. GIS for health organizations. ESRI Press: Redlands, Calif. 2000. McCormick RJ, Smith R, Edwards D, White D, Langford J. The distribution of general dental practitioners with NHS contract numbers in relation to the distance of their practices from the seven dental undergraduate teaching hospitals in England outside London. Community Dent Health 2008; 25: 201-204. Parker RN. GIS and spatial analysis for the social sciences : coding, mapping and modeling. Routledge: New York 2008. Steinberg SJ. GIS : geographic information systems for the social sciences : investigating space and place. SAGE Publications: Thousand Oaks, Calif. 2006. |
| List of Research Outputs |
| Bootvong K., Liu Z., McGrath C.P.J., Hagg E.U.O., Wong R.W.K., Bendeus S.A.M. and Yeung S., Virtual model analysis as an alternative approach to plaster model analysis: reliability and validity, The European Journal of Orthodontics. 2010, 32: 589-595. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience - Journal Based Learning and Fundamentals of Dental Research – a pilot programme, the 6th International Conference on PBL in Dentistry. Hong Kong, 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience-journal based learning and fundamentals of dental research-a pilot programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.26. |
| Bridges S.M., Botelho M.G., Dyson J.E., McGrath C.P.J., Tsang P.C.S. and Yiu C.K.Y., Blended learning and interactive pedagogies: Supporting problem-based learning in dental education, CITE Research Symposium 2010. 2010. |
| Bridges S.M., McGrath C.P.J., Wong H.M. and Au T.K.F., Exploring The Dimensions Of Oral Health Literacy. , In: Bridges, S. M., McGrath, C. P. J., Wong, H. M., & Au, T. K. , The 9th World Congress On Preventive Dentistry, Phuket, Thailand.. 2009. |
| Bridges S.M., Corbet E.F., Samaranayake L.P., Dyson J.E., Leung W.K. and McGrath C.P.J., Improving programme evaluations: The effect of curriculum review, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 37. |
| Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., Multilingual clinical interactions in Dentistry: A microanalysis, 11th International Pragmatics Conference. Australia, 2009. |
| Bridges S.M., McGrath C.P.J., Yiu C.K.Y. and Cheng S.S., Reassuring' during clinical examinations: Novice and expert talk in dentistry, Journal of Asian Pacific Communication. John Benjamins, 2010, 20:2: 185-206. |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Choi W.W.S., Lee S..., McGrath C.P.J. and Samman N., Change in quality of life after combined orthodontic-surgical treatment of dentofacial deformities, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2010, 109 (1): 46-51. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Du R.Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health- and oral health-related quality of life among preschool children with cerebral palsy, Quality of Life Research. 2010, 19: 1367-1371. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Du RY, McGrath C, Yiu CKY, King NM. Health-related and oral health-related quality of life among preschool children with cerebral palsy. Best Poster Presentation , Conjoint scientific sessions – Faculty of Dentistry, the University of Hong –Peking University School of Stomatology, 16-17 November 2009. 2009. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health and oral health-related quality of life among preschool children with Cerebral Palsy, 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health-related and oral health-related quality of life among preschool children with cerebral palsy. , Conjoint Scientific Session. The University of Hong Kong-Peking University School of Stomatology, 16-17 November, 2009. p.32. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Oral health in the primary dentition of children with cerebral palsy. , 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Gao X., McGrath C.P.J. and Lin H.C., Oral Health Status of Rural-Urban Migrant Children in South China, International Dental Association for Dental Research 88th General Session. 2010. |
| Hagg E.U.O., Khanoengnit B., Liu Z., McGrath C.P.J. and Wong R.W.K., Virtual models as an alternative approach to plaster model assessment of tooth dimension, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p106 (SP284). |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and Responsiveness of ChineseECOHIS to Dental Treatment, 1st Hong Kong International Dental Expo and Symposium. 2010. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and responsiveness of the Chinese version of the Early Childhood Oral Health Impact Scale (ECOHIS) to dental treatment under general anaesthesia. , Conjoint Scientific Session 2009. The University of Hong Kong-Peking University School of Stomatology, 16-17 Kong November 2009. p.32. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Translation and validation of a Chinese language version of the Early Childhood Oral Health Impact Scale (ECOHIS), International Journal of Paediatric Dentistry. 2009, 19: 399-405. |
| Lu H.X., Wong M.C.M., Lo E.C.M. and McGrath C.P.J., Factors associated with oral health from childhood to early adulthood, Journal of Dental Research. 2009, 88 (Spec Iss B): 313 (PAPF/APR). |
| McGrath C.P.J., Zhang W. and Lo E.C.M., A review of the effectiveness of oral health promotion activities among elderly people, Gerodontology. 2009, 26: 85-96. |
| McGrath C.P.J., Invited lectures, "The value and use of quality of life measures in orthodontics", "Selecting appropriate quality of life measures for orthodontics", and "Interpreting qualify of life data in clinical practice and research", Finnish Orthodontic Society, Helsinki, Finland, March 26-27. 2010. |
| McGrath C.P.J., Cheung K., Hong P.Y., Hui B.S.Y., Leung C.Y., Leung K.W., Tang C.F., Tse Y.K., Wang Z. and Wong H.C., Post-traumatic stress and oral health among asylum seekers in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 169: 1-38. |
| Verayannont P., Hagg E.U.O., Wong R.W.K., McGrath C.P.J. and Yeung S., Maxillary growth during puberty determined by the implant method compared with cephalometry, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p70 (SP119). |
| Wong M.C.M., Lu H.X., Lo E.C.M. and McGrath C.P.J., Oral health status of young adults in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 308 (PAPF/APR). |
| Wu A., McGrath C.P.J., Wong R.W.K., Wiechmann D. and Rabie A.B.M., Pain and daily oral impact and satisfaction of patients treated with labial and lingual fixed appliances, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p18 (OP033). |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath C.P.J., Comprehensive Cephalometric Analyses of 10 to 14-Year Old Southern Chinese, The Open Anthropology Journal 2010. 2010, 3: 85-95. |
| Zhang W., McGrath C.P.J. and Lo E.C.M., A comparison of root caries diagnosis based on visual-tactile criteria and DIAGNOdent in vivo, Journal of Dentistry. 2009, 37: 509-513. |
| Zhu H., McMillan A.S. and McGrath C.P.J., Oral health-related quality of life after stroke, Journal of Dental Research. 2009, 88 (Spec Iss B): 610 (PAPF/APR). |
| Researcher : Mcmillan AS |
| Project Title: | Orofacial Pain and its Functional and Psychosocial Impact |
| Investigator(s): | McMillan AS, Wong MCM, Lam CLK, Luo Y |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2005 |
| Abstract: |
| This is a cross-disciplinary study involving the Faculties of Dentistry and Medicine that is directly related to the major research theme of Healthy Ageing. Hypotheses: o A substantial number of community-dwelling adults in Hong Kong suffer from orofacial pain (OFP) and the majority of them do not seek professional help for their condition o OFP is an under-treated condition that causes significant disability and has a serious adverse effects on people's well-being and quality of life. Purpose: To investigate a) the prevalence of OFP and its diagnostic sub-groups in community dwelling adult people in Hong Kong, b) the adverse impact of OFP on functional and psychosocial wellbeing, and c) barriers to treatment seeking for OFP symptoms. Objectives: o To determine the frequency, severity and pattern of OFP according to diagnostic sub-groups using validated patient-centred measures and clinical assessment o To describe the treatment seeking behaviour associated with OFP and factors associated with such behaviour o To assess the impact of OFP on health-related QoL using generic and condition specific health status measures o To correlate patient descriptions of OFP with clinical diagnoses Pain is closely associated with poor health. The negative effect of pain on health outcomes such as function and quality of life (QoL) among patient populations is well documented (Ahmedzai, 1995). However, little is known about the impact of pain on subjective health and well-being in community dwelling populations (Reyes-Gibby et al, 2002). Although pain is a common symptom of dental and oral disease and can have a significant impact, there is a shortage of systematic data on the prevalence, severity and consequences of orofacial pain (OFP) in general, non-clinical populations (Nikias, 1985). In a community based study in Toronto, Locker & Grushka (1987) noted that 53% of participants had experienced some pain or discomfort in the orofacial region in the previous four weeks. The majority of pain reports were tooth or gum related whereas pain in the jaws, face and oral mucosa were less common. Epidemiological studies in the USA, Canada and the United Kingdom have shown that the prevalence of oro-facial pain symptoms in adult populations ranges from 14 to 40% (Lipton et al, 1993; Locker & Grushka, 1987; Macfarlane et al 2002). Gender and age-related differences in oro-facial pain prevalence have also been noted with women and younger adults at apparently greater risk of pain symptoms, whereas older people are more likely to suffer more severe pain (Macfarlane et al, 2002; Von Korff et al, 1988). Pain may have considerable impact on people's ability to perform effectively at work, home and school. Nonetheless, not everyone seeks professional help for pain with some symptoms being ignored while others may prompt action (Macfarlane et al, 2003). It is, therefore, important to discern factors that may distinguish individuals who seek professional care from those who do not. In a population-based study of OFP in the UK, Macfarlane et al (2003) noted that less than half of their study population with OFP sought professional advice. The majority of factors associated with seeking treatment appeared to be various characteristics of pain although the time course of these factors was unclear. Locker & Grushka (1987) observed that while more than 50% of their OFP study group described pain severity as moderately severe or severe, only 40 % of those reporting pain sought advice from a doctor of dentist. Ethnic differences in the perception of and means of managing pain account, at least in part, for observed differences in treatment seeking behaviour for orofacial pain conditions (Hastie et al, 2005). Socioeconomic status is also an important factor related to treatment seeking with oral disease generally more common among those least able to access health care and leads to needless pain and suffering (Riley et al, 2005). There is presently very little information on the prevalence and impact of oro-facial pain in Southern Chinese people in Hong Kong. Most oro-facial pain prevalence studies have focused on predominantly Caucasian populations. However, a recent study in Korean elders indicated that the prevalence was higher in this ethnic group and significant disability was associated with the condition (Chung et al, 2004). In a previous population-based survey, we have shown that self-reported pain symptoms associated with temporomandibular disorders are relatively common in the Hong Kong Chinese population (Pow et al, 2001). However, the characteristics of the pain differed from other ethnic groups, no gender bias in pain symptoms was observed and treatment seeking was much lower than in Caucasian counterparts. One of the problems associated with the available epidemiological data on prevalence of OFP is that it has been collected solely by means of questionnaires with patient self-reports not being confirmed by clinical assessment (Macfarlane et al, 2004). There is, therefore, the risk that patient described pain symptoms are misinterpreted and pain symptoms due to multiple conditions may go unrecognized. Definitive diagnoses can only be obtained by clinical assessment. References: Ahmedzai S. Recent clinical trials of pain control: impact on quality of life. Eur J Cancer 1995;31A(Suppl):S2-7 Chung JW, Kim JH, Kim HD, Kho HS, Kim YK, Chung SC. Chronic orofacial pain among Korean elders: prevalence, and impact using the graded chronic pain scale. Pain 2004;112:164-170 Hastie BA, Riley III JL, Fillingim RB. Ethnic differences and responses to pain in healthy young adults. Pain Medicine 2005;6:61-71 Lipton JA, Ship JA, Larach-Robinson D. Estimated prevalence and distribution of reported orofacial pain in the United Sates. J Am Dent Assoc 1993;124:115-121 Locker D, Grushka M. Prevalence of oral and facial pain and discomfort: preliminary results from a mail survey. Community Dent Oral Epidemiol 1987;15:169-172 Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV. Factors associated with health care seeking behaviour for orofacial pain in the general population. Community Dent Health 2003;20:20-26 Macfarlane TV, Blinkhorn AS, Craven R et al. Can one predict the likely specific orofacial pain syndrome from a self-completed questionnaire? Pain 2004;111:270-277 Nikias M. Oral disease and the quality of life. Am J Public Health 1985;75:11-12 Reyes-Gibby C, Aday L, Cleeland C. Impact of pain on self-related health in community-dwelling older adults. Pain 2002;95:75-82 Riley III JL, Gilbert GH. Orofacial pain symptoms: an interaction between age and sex. Pain 2001;90:245-256 Von Korff M, Ormel J, Keefe FJ, Dworkin SF. Grading the severity of chronic pain complaints. Pain 1988;32:173-183 |
| Project Title: | Predictors of outcome of orofacial pain: A community-based longitudinal study |
| Investigator(s): | McMillan AS, Wong MCM, Lam CLK, Luo Y, Zheng J |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2006 |
| Completion Date: | 10/2009 |
| Abstract: |
| This is a cross-disciplinary study involving the Faculties of Dentistry and Medicine that is directly related to the major research theme of Healthy Ageing. Purpose: To investigate a) the pain characteristics in community-dwelling adult Hong Kong Chinese people with OFP, b) to follow-up OFP sufferers over a 6-month period and identify presenting factors and behaviours that predict poor outcome. Null hypotheses: - Orofacial pain does not tend to persist - There are no factors that can predict the persistence of OFP Objectives: o To describe the outcome of OFP after a period of 6-months o To identify baseline factors that predict poor outcome in adults with OFP Orofacial pain (OFP) may be defined as pain related to the face and/or mouth and involves both hard and soft tissues in these anatomical regions. Epidemiological studies in the USA, Canada and the United Kingdom have shown that the prevalence of OFP symptoms in adult populations ranges from 14 to 40%.1-3 In a population-based survey of OFP symptoms in adult Chinese people in Hong Kong, the one-month period prevalence of OFP (42%) was found to be consistent with estimates in western countries.4 Pain is closely associated with poor health and may considerably impair people's ability to perform daily living activities. Despite this, not all pain sufferers seek professional help with some symptoms being ignored whilst others prompt attention. (2,3,5,6). In studies involving western cohorts 44-46% with OFP sought professional help (2,5,6). However, only 20% of Hong Kong Chinese adults with OFP symptoms sought professional treatment.4 Thus, it is important to explore the factors and barriers relating to professional treatment seeking behaviour in order to understand the disparity between Chinese adults and their western counterparts. MacFarlane et al5 noted that pain characteristics appeared to prompt treatment seeking although the time course of these factors was unclear. The decision to seek treatment may be related to the consequences of the OFP as experienced by the individual and also affected by culturally-mediated responses to the pain experience and its consequences (7). A key characteristic of OFP is that it tends to persist (8). In a longitudinal study in the United Kingdom, over half those with OFP at baseline reported pain four years later (9). Persistent pain was associated with females, increasing age, psychological distress, widespread body pain and taking pain medication at baseline. In a Swedish cohort, the follow-up of 109 patients with OFP revealed that after 4 to 9 years only 27% had resolution of the pain, with pain medication usage at baseline a strong predictor of persistent OFP (10). The consequences of OFP have an impact on various aspects of daily life and include work loss, sleep disturbance, need for more rest, avoidance of social contacts, and difficulty eating and speaking (2,11). The magnitude of the impact appears to be related to the pain characteristics and socio-demographics of the respondents (2,3,11). Pain-related sleep disturbance is also a key determinant of seeking professional treatment (6). There is scant information available on the characteristics of OFP in southern Chinese people, its potential to persist and the functional and psychosocial impact. In a population-based survey of symptoms of temporomandibular disorders in Hong Kong Chinese people, jaw pain, restricted jaw opening and jaw clicking were the most common presenting symptoms and often associated with significant morbidity (12). In contradistinction to western studies, there were no gender-related differences in symptom reporting or treatment seeking (12). In a recent study of chronic OFP in elderly Koreans the prevalence appeared to be higher compared with Caucasian populations.13Adverse impacts were common with around 30% of those with joint pain, toothache and burning mouth symptoms having significant associated disability and impairment of daily living activities (13). The ideal approach to the investigation of OFP in the community is by interview using validated questionnaires and clinical assessment. However, most studies where patient-based and clinical data are available involve a bias sample of people seeking treatment. In epidemiological studies of OFP, the most common means of obtaining information is by questionnaire, by mail or telephone survey, without clinical examination of the participants. Even with contemporary questionnaire approaches, without clinical assessment, there is always the issue of data reliability. References 1. Lipton JA, Ship JA, Larach-Robinson D. Estimated prevalence and distribution of reported orofacial pain in the United States. J Am Dent Assoc 1993;124:115-21. 2. Locker D, Grushka M. Prevalence of oral and facial pain and discomfort: preliminary results of a mail survey. Community Dent Oral Epidemiol 1987a;15:169-72. 3. Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV (2002). Oro-facial pain in the community: prevalence and associated impact. Community Dent Oral Epidemiol 30(1):52-60. 4. McMillan AS, Wong MCM, Zheng J, Lam CLK. Prevalence of orofacial pain and treatment seeking in Hong Kong Chinese. J Orofac Pain (in press) 5. Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV. Factors associated with health care seeking behaviour for orofacial pain in the general population. Community Dent Health 2003;20:20-26. 6. Locker D. The symptom iceberg in dentistry. Treatment-seeking in relation to oral and facial pain. J Can Dent Assoc 1988:54:271-4. 7. Hastie BA, Riley JL, Fillingim RB. Ethnic differences and responses to pain in healthy young adults. Pain Med 2005;6:61-71. 8.Von Korff M, Ormel J, Keefe F, Dworkin S (1992). Grading the severity of chronic pain. Pain 50(2):133-149. 9.Macfarlane TV, Blinkhorn AS, Davies RM, Kincey J, Worthington HV. Predictors of outcome for orofacial pain in the general population: a four-year follow-up study. 2004 J Dent Res ;83:712-7. 10. Wolf E, Nilner M, Petersson A, Petersson K (2002). Long-term follow-up by means of a questionnaire of 109 patients with long-lasting orofacial pain. Swed Dent J. 26(3):125-34. 11. Locker D, Grushka M. The impact of dental and facial pain. J Dent Res 1987;66:1414-7. 12. Pow EHN, Leung KCM, McMillan AS. Prevalence of symptoms associated with temporomandibular disorders in Hong Kong Chinese. J Orofac Pain 2001;15:228-34. 13. Chung JW, Kim JH, Kim HD, Kho HS, Kim YK, Chung SC. Chronic orofacial pain among Korean elders: prevalence, and impact using the graded chronic pain scale. Pain 2004;112:164-70. |
| Project Title: | A randomized clinical trial on the effect of Chlorhexine mouth rinse and assisted tooth brushing on the health condition and qulaity of life of elderly stroke patients |
| Investigator(s): | McMillan AS, McGrath CPJ, Li LSW, Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2007 |
| Abstract: |
| The main purpose of this oral health intervention project is to compare the effect of providing 1) professional oral hygiene instruction alone versus 2) professional oral hygiene instruction plus adjunctive Chlorhexidine mouth rinse versus 3) professional oral hygiene instruction plus adjunctive assisted tooth brushing and Chlorhexidine mouth rinse, on the oral health condition, the general health condition and health-related quality of life (HRQoL) of patients with stroke who are receiving hospital-based rehabilitation. An additional purpose is to assess potential longer term oral intervention-related health and HRQoL outcomes. Null hypotheses: 1. There is no difference in a) the amount of dental plaque, gingival bleeding and oral carriage of yeasts and coliforms, b) morbidity or mortality levels, and c) the change in HRQoL between stroke patients who have received professional oral hygiene instruction alone compared with professional oral hygiene instruction plus adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. 2. There is no difference in the change in health condition and HRQoL among the three treatment groups, 6-months after completion of the oral intervention. Objectives: To describe and compare prospectively over a 4-week period during hospital-based rehabilitation, 1a. Changes in the amount of dental plaque, gingival bleeding and oral carriage of yeasts and coliforms in stroke patients after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. b. Changes in morbidity and mortality levels in stroke patients after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. c. Changes in HRQoL in stroke patients after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. 2. To describe and compare changes in health condition and HRQoL in stroke patients 6-months after receiving professional oral hygiene instruction with or without adjunctive assisted tooth brushing and/or Chlorhexidine mouth rinse. Stroke is one of the commonest causes of brain injury in the elderly and leads to significant disability and mortality (Counsell et al, 1995). Depending on the severity and type, stroke leaves an individual with residual impairment of physical, psychological and social functions. Limb paralysis and reduced manual dexterity are common physical features (Scully & Cawson, 1998). Up to 85% of stroke patients have an initial deficit in the arm and hand and problems remain in 55-75% of patients three to six months later (Lai et al, 2002). Oro-facial manifestations of stroke include unilateral facial palsy that mainly affects the lower face, tongue and soft palate. Stroke-related oral motor impairment is very disabling as speech, mastication and swallowing problems are common and such key behaviours do not always fully recover (Smithard, 2001). Impaired manual dexterity affects the stroke survivors’ ability to clean their teeth and dentures and maintain a healthy oral condition and together with impaired oral function-related inability to clear the mouth of food debris result in halitosis, caries and an increased risk of oral microbial infections notably Candida and coliform species (Rose et al, 2002). Tooth brushing is the mainstay of good oral hygiene and health. However, after stroke, conventional tooth brushing is difficult and often ineffective. Battery-operated tooth brushes are more effective than conventional tooth brushes for disabled patients (Kamen, 1997). Even then, good oral hygiene may be difficult to attain without assistance from a care-giver. A combination of mechanical and chemical interventions have proven efficacy in the prevention of periodontal diseases (Santos et al, 2004). Chlorhexidine, a bisguanide, is considered to be the most effective chemical agent in dental plaque control and is used routinely in mouth rinse form as an adjunct in the treatment of periodontal diseases (Van Strydonck et al, 2005). It displays low toxicity and microbial resistance and supra-infection do not appear to occur, and its persistence prolongs anti-bacterial action (Paraskevas, 2005). Measurement of health related quality of life (HRQoL) in stroke sufferers provides information on recovery, identifies predictors of patient outcome and assists in the evaluation of interventions (Pickard et al, 1999). An effective approach to the measurement of quality of life is a combination of generic and specific questionnaires (Allen, 2003). The Medical Outcomes Short-Form Health Survey questionnaire (SF-36) is a generic measure that has been validated for use in stroke patients (Dorman et al, 1999). The SF-12 also exhibits an acceptable level of responsiveness to change in stroke patients (Muller-Nordhorn et al, 2005). The Oral Health Impact Profile (OHIP-49) and its short form version are sophisticated oral specific instruments that measure the impact of oral conditions on HRQoL (Allen, 2003). References: Allen PF. Assessment of oral health related quality of life. Health Qual Life Outcomes. 2003 Sep 8;1:40 Counsell C, Wardlow C, Sandercock P et al. The Cochrane Collaboration Stroke Review Group. Meeting the need of systematic reviews in stroke care. Stroke 1995;26:498-502 Dorman PJ, Dennis M, Sandercock P. How do scores on the EuroQol relate to scores on the SF-36 after stroke? Stroke 1999;30:2146-2151 Kamen S. Oral health care for the stroke survivor. J California Dent Assoc 1997;25:297-303 Lai SM, Studenski S, Duncan PW, Perera S. Persisting consequences of stroke measured by the stroke impact scale. Stroke 2002;33:1840-1844 Muller-Nordhorn J, Nolte CH, Rossnagel K et al. The use of the 12-item short-form health status instrument in a longitudinal study of patients with stroke and transient ischaemic attack. Neuroepidemiology 2005;24:196-202 Parakevas S. randomized controlled clinical trials on agents used for chemical plaque control. Int J Dent Hygiene 2005;3:162-178 Pickard AS, Johnson JA, Penn A et al. Replicability of SF-36 summary scores by the SF-12 in stroke patients. Stroke 1999;30:1213-1217 Rose LF, Mealey B, Minsk L, Cohen W. Oral care for patients with cardiovascular disease and stroke. JADA 2002;133:375-345 Santos S, Herrera D, Lopez E, O’Connor A et al. A randomized clinical trial on the short-term clinical and microbiological effects of the adjunctive use of 0.05% chlorexidine mouth rinse for patients in supportive periodontal care. J Clin Periodontol 2005;31:45-51 Scully C, Cawson RA. Medical Problems in Dentistry. Wright, UK, 1998 Smithard DG. Swallowing and stroke. Neurological affects and recovery. Cerebrovasc Dis 2002;14:1-8 Van Strydonk DAC, Timmerman MF, van der Velden U et al. Plaque inhibition of two commercially available chlorhexidine mouthrinses. J Clin Periodontol 2005;32:30530-9 |
| Project Title: | 86th General Session & Exhibition of the International Association for Dental Research (IADR) Treatment seeking behaviour in Southern Chinese people with orofacial pain |
| Investigator(s): | McMillan AS |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 07/2008 |
| Abstract: |
| N/A |
| List of Research Outputs |
| McMillan A.S., Wong M.C.M., Zheng J., Luo Y. and Lam C.L.K., Widespread pain symptoms and psychological distress in southern Chinese with orofacial pain, Journal of Oral Rehabilitation. 2010, 37: 2-10. |
| Perera R.A.P.M., Tsang P.C.S., McMillan A.S., Miller C.S., Lee M.P., Li P. and Samaranayake L.P., Oral chemokine interactome unfolds potential anti-HIV mucosal microbicide targets, 23rd IADR Annual Scientific Meeting . 2009. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., A comparison of laboratory extraction techniques for the detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients., Oral Oncology. 2009, 3: 200. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., Detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients: a comparison of two laboratory extraction techniques, Conjoint Scientific Sessions Faculty of Dentistry, The University of Hong Kong - Peking University School of Stomatology. 2009. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Zheng J., McMillan A.S., Wong M.C.M., Luo Y. and Lam C.L.K., Investigation of factors associated with treatment-seeking in southern Chinese with orofacial pain, Journal of Orofacial Pain. 2010, 24: 181-188. |
| Zhu H., McMillan A.S. and McGrath C.P.J., Oral health-related quality of life after stroke, Journal of Dental Research. 2009, 88 (Spec Iss B): 610 (PAPF/APR). |
| Researcher : Mei L |
| List of Research Outputs |
| Chu C.H., Mei L., Lo E.C.M., Seneviratne C.J. and Yip H.K., Effects of silver diamine fluoride on mono-species cariogenic biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 363 (PAPF/APR). |
| Chu C.H., Mei L. and Lo E.C.M., Use of fluorides in dental caries management, General Dentistry. 2010, 58: 37-43. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Action of Silver Diamine Fluoride on Dentin Caries [Abstract], First Hong Kong International Dental Expo and Symposium; Hong Kong Dental Association. 2010, In Abstracts of papers: 84. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Effects of multi-species biofilm dentin caries-like lesions in artificial mouth, Journal of Dental Research. 2010, 89 (Spec Iss A): 1393 in AADR 39th Annual Meeting. |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., Effects of silver-diamine fluoride on caries-like lesion and cariogenic biofilm, Journal of Dental Research. 2009, 88 (Spec Iss B): 40 (PAPF/APR). |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., International Association for Dental Research / Unilever Hatton Travel Award for M.Lei (co-author), 23nd Annual Scientific Meeting, Southeast Asian Division of International Association for Dental Research 2009. . 2009. |
| Researcher : Mei L |
| List of Research Outputs |
| Chu C.H., Mei L., Lo E.C.M., Seneviratne C.J. and Yip H.K., Effects of silver diamine fluoride on mono-species cariogenic biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 363 (PAPF/APR). |
| Chu C.H., Mei L. and Lo E.C.M., Use of fluorides in dental caries management, General Dentistry. 2010, 58: 37-43. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Action of Silver Diamine Fluoride on Dentin Caries [Abstract], First Hong Kong International Dental Expo and Symposium; Hong Kong Dental Association. 2010, In Abstracts of papers: 84. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Effects of multi-species biofilm dentin caries-like lesions in artificial mouth, Journal of Dental Research. 2010, 89 (Spec Iss A): 1393 in AADR 39th Annual Meeting. |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., Effects of silver-diamine fluoride on caries-like lesion and cariogenic biofilm, Journal of Dental Research. 2009, 88 (Spec Iss B): 40 (PAPF/APR). |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., International Association for Dental Research / Unilever Hatton Travel Award for M.Lei (co-author), 23nd Annual Scientific Meeting, Southeast Asian Division of International Association for Dental Research 2009. . 2009. |
| Researcher : Mo S |
| List of Research Outputs |
| You M., Mo S., Leung W.K. and Watt R.M., Diversity of oral treponemes in patients with chronic periodontitis, Journal of Dental Research. 2009, 88 (Spec Iss B): 377 (PAPF/APR). |
| Researcher : Newsome PRH |
| Project Title: | All-porcelain crowns in general dental practice. A qualitative and clinical study |
| Investigator(s): | Newsome PRH |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2006 |
| Completion Date: | 10/2009 |
| Abstract: |
| Restorative dentistry has changed dramatically in recent years. The introduction of a variety of indirect all-porcelain crown systems (eg Empress II. Cercon, Procera) has allowed dentists to place restorations that are not only aesthetic but also durable and conservative. Case selection, material choice, appropriate tooth preparation and cementation of such restorations are, however, considered to be critical and demand close adherence to a set of clinical principles if they are to be successful. Little is known of a) dentists' views and accordingly the willingness of dentists to offer them to their patients and b) the clinical ability of dentists to conform to the aforementioned principles. The aim of this research is to explore the use of such restorations in dental practice. It will also examine the level and nature of communication between dentists and laboratories, widely viewed as a critical determinant of success. Specificically, it is proposed to examine Hong Kong dentists': 1) opinions of, and experience with, the new generation of all-porcelain restorations 2) understanding of accepted principles regarding case selection and tooth preparation with respect to all-porcelain restorations 3) communication with the dental laboratory 4) assessment of all-porcelain restorations supplied by the dental laboratory |
| Project Title: | British Dental Conference & Exhibition Loss Leaders in Dentistry - A Fresh Look at an Old Idea |
| Investigator(s): | Newsome PRH |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 05/2007 |
| Abstract: |
| N/A |
| Project Title: | British Dental Conference & Exhibition (Manchester 2008) Old Dentistry New Dentistry. Is There A Difference? |
| Investigator(s): | Newsome PRH |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 05/2008 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Newsome P.R.H., A phoenix from the flames, Private Dentistry. UK, 2010, January: 58. |
| Newsome P.R.H., Reaney D. and Owen S., A systematic approach to success, Irish Dentist. UK, 2010, 13: 33-34. |
| Newsome P.R.H., Aesthetic treatment options for anterior teeth, British Dental Conference and Exhibition. 2010. |
| Newsome P.R.H., Back to basics, Private Dentistry. 2009, July: 71. |
| Newsome P.R.H., Business risks in good times and bad, Dental Protection The Premier Symposium. 2009. |
| Newsome P.R.H., Reaney D. and Penn D., Case study: a new generation PFZ crown, Irish Dentist. 2009, Oct: 15-17. |
| Newsome P.R.H., Common courtesy, Private Dentistry. 2009, October: 64. |
| Newsome P.R.H., Don't make me laugh, Private Dentsitry. 2010, May: 50. |
| Newsome P.R.H., Editorial Board Member, Private Dentistry. 2009. |
| Newsome P.R.H., Education: reaping what we sow, Private Dentistry. 2009, November: 64. |
| Newsome P.R.H., Keep calm and don't panic, Private Dentistry. 2009, December: 64. |
| Newsome P.R.H., Reaney D. and Owen S., Marketing with a difference, Irish Dentist. UK, 2010, 13: 28-32. |
| Newsome P.R.H., Old Dentistry, New Dentistry, Denplan National Conference. 2009. |
| Newsome P.R.H., Porcelain veneers - A personal perspective, General Dental Practitioner group, Enniskillen, UK. 2009. |
| Newsome P.R.H., Reaney D. and Owen S., Putting a price on your dentistry, Irish Dentist. UK, 2010, 13: 22-25. |
| Newsome P.R.H., Restorative Dentistry - Then and Now, Faculty of General Dental Practice (UK) Yorkshire Masterclass. 2009. |
| Newsome P.R.H., Restorative dentistry - then and now, FGDP (UK) Yorkshire Division. 2009. |
| Newsome P.R.H., Reaney D. and Penn D., Revisiting cast gold restorations, Irish Dentist. 2009, November: 30-32. |
| Newsome P.R.H., Stop comparing yourself to others, Private Dentistry. 2009, September: 76. |
| Newsome P.R.H., Taking the right steps, Private Dentistry. 2010, June: 58. |
| Newsome P.R.H., Reaney D. and Penn D., Technology Update: the clinical-laboratory interface Part Nine. Successful ceramic veneers, Irish Dentist. 2009, September: 20-23. |
| Newsome P.R.H., Reaney D. and Penn D., Technology update: the clinical-laboratory interface Part Eight. The surface treatment of indirect restorations , Irish Dentist. 2009, July: 18-20. |
| Newsome P.R.H., The business and practice of aesthetic dentistry, Chester and Cardiff, UK, General dental practitioner groups. 2010. |
| Newsome P.R.H., The business of dental practice. One day postgraduate course, HKU, 2010. |
| Newsome P.R.H., Reaney D. and Owen S., The importance of leadership, Irish Dentist. UK, 2010, 13: 24-26. |
| Newsome P.R.H., Reaney D. and Owen S., Understanding the business you are in, Irish Dentist. UK, 2010, 13: 24-26. |
| Newsome P.R.H., What not to wear, Private Dentistry. UK, 2010, February: 66. |
| Newsome P.R.H., What's in a name?, Private Dentistry. 2010, April: 61. |
| Newsome P.R.H., Working with your spouse, Private Dentistry. 2010, March: 77. |
| Researcher : Ng AFS |
| List of Research Outputs |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia . 2010, March/April 2010: 21-27. |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia November/December 2009. 2009, 24-29. |
| Researcher : Ng KS |
| List of Research Outputs |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Researcher : Ng SKS |
| List of Research Outputs |
| Leung W.K., Chu C.H., Mok T.M.Y. and Ng S.K.S., Periodontal status of adults with systemic sclerosis in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 655 (PAPF/APR). |
| Researcher : O'Donnell D |
| Project Title: | 79th General Session & Exhibition of the IADR Special Needs Dentistry within the Dental Curriculum |
| Investigator(s): | O'Donnell D |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2001 |
| Abstract: |
| N/A |
| Project Title: | Summer Conference of the British Society for Disability and Oral Health Special Needs Dentistry in the Undergraduate and Postgraduate Curriculum: The Hong Kong Experience |
| Investigator(s): | O'Donnell D |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 05/2002 |
| Abstract: |
| N/A |
| Researcher : Ow ATC |
| List of Research Outputs |
| Cheung L.K., Ow A.T.C. and Chua H.D.P., Simultaneous modified oblique Le Fort III and segmentalized Le Fort I osteotomies, Journal of Oral and Maxillofacial Surgery. 2010, 68: 915 - 923. |
| Researcher : Ow T-CA |
| List of Research Outputs |
| Cheung L.K., Ow T.-.C.A. and Chua H.D.P., Simultaneous modified oblique Le Fort III and segmentalized Le Fort I osteotomies, J Oral Maxillofac Surg. 2010, 68: 915-23. |
| Ow T.-.C.A. and Cheung L.K., Skeletal stability and complications of bilateral sagittal split osteotomies and mandibular distraction osteogenesis: an evidence based review, International Journal of Oral and Maxillofacial Surgery. 2009, 67: 2344 - 2353. |
| Researcher : Pang HH |
| List of Research Outputs |
| Liu J.K.S., Pang H.H., Li K.Y., Cheung C.K.L., Fung J., Ho R.C.Y., Lam A.K.Y., Lee C.T.Y., Ng A.K.C., Sia A.O.N., Wong J.C.L. and Yung A.K.C., The dental product advertisement in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 168: 1-68. |
| Researcher : Parahitiyawa NB |
| List of Research Outputs |
| Parahitiyawa N.B., Scully C., Leung W.K., Yam W.C., Jin L.J. and Samaranayake L.P., Exploring the oral bacterial flora: current status and future directions, Oral Diseases. 2010, 16: 136-145. |
| Researcher : Peng X |
| List of Research Outputs |
| Peng X., Mao C... and Yu G...Y..., Comparison of three kinds of soft tissue free flaps for oral-maxillofacial defects reconstruction, The 2009 International & the 10th National Head & Neck Cancer Conference, September 18-10, 2009, Tianjin, China. 2009, 271-272. |
| Peng X., Mao C... and Yu G...Y..., Soft tissue free flaps for oral and maxillofacial defects reconstruction, The 8th National Oral & Maxillofacial - Head & Neck Cancer Conference, September 11-13, 2009. Weihai, China, . 2009, 12-13. |
| Wang D...C..., Guo C...B..., Peng X., Su Y...J... and Chen F..., Attitudes towards truth-telling about cancer: a survey of patients and their families with oral and maxillofacial cancer, Chinese Journal of Hospital Administration. 2009, 25 (11): 769-711. |
| Yan Y...B..., Mao C..., Peng X., Yu G...Y..., Guo C...B... and Luan X...W..., Evaluation quality of life in patients with oral squamous cell carcinoma using UW-QOL, China Journal of Oral and Maxillofacial Surgery. 2009, 7 (6): 495-499. |
| Yan Y...B..., Mao C..., Peng X., Guo C...B..., Zhang J...G... and Yu G...Y..., Preliminary evaluation of quality of life in 97 patients with oral cancer before surgery, China Journal of Oral and Maxillofacial Surgery. 2009, 7(5): 411-417. |
| Yan Y...B..., Mao C..., Peng X., Yu G...Y..., Guo C...B... and Zou S...Q..., Psychometric properties of Chinese version SF-36 for measuring quality of life in patients with oral cancer, Journal of Modern Stomatology. 2009, 23 (6): 561-563. |
| Yan Y...B..., Mao C..., Peng X., Yu G...Y..., Guo C...B... and Zou S...Q..., Quality-of-life evaluation after microvascular oromandibular reconstruction: a comparative study of reconstructed and nonreconstructed patients, Journal of Modern Stomatology. 2009, 23 (5): 449-452. |
| Yang Z...C..., Wang X..., Yi B..., Li Z...L..., Mao C... and Peng X., Correction of hemifacial atrophy with free flaps, Chinese Journal of Plastic Surgery. 2009, 25 (4): 290-291. |
| Yu G...Y..., Ma D...Q..., Gao Y..., Peng X., Guo C...B..., Huang M...X..., Sun K...H..., Wu Q...G... and Mao C..., Functional surgical treatment of salivary gland tumors. Second Prize of Science and Technology Award, Ministry of Education, China, 2009. 2009. |
| Yu G...Y..., Ma D...Q..., Gao Y..., Peng X., Guo C...B..., Huang M...X..., Sun K...H..., Wu Q...G... and Mao C..., New techniques for the treatment of salivary gland tumors. First Prize of Science and Technology Award, Chinese Anti-Cancer Association, 2009. 2009. |
| Zhang L..., Xu H..., Cai Z...G..., Mao C..., Wang Y..., Peng X., Zhu Z...H... and Yu G...Y..., Clinical and anatomic study on the ducts of the submandibular and sublingual glands, Journal of Oral and Maxillofacial Surgery. 2010, 68 (3): 606-610. |
| Zhou M..., Peng X., Mao C..., Xu F..., Hu M... and Yu G...Y..., Primate mandibular reconstruction with prefabricated, vascularized tissue-engineered bone flaps and recombinant human bone morphogenetic protein-2 implanted in situ, Biomaterials. 2010, 31 (18): 4935 - 4943. |
| Researcher : Perera RAPM |
| List of Research Outputs |
| Perera R.A.P.M., Tsang P.C.S., Chan K.H., Lee M.P., Li P., Miller C.S. and Samaranayake L.P., Cytokine Interactome Alterations Promote Oropharyngeal Epstien-barr Virus (EBV) Replication During HIV Infection, Gene Expression Omnibus (GEO) Series GSE21339. 2010. |
| Perera R.A.P.M., Tsang P.C.S., McMillan A.S., Miller C.S., Lee M.P., Li P. and Samaranayake L.P., Oral chemokine interactome unfolds potential anti-HIV mucosal microbicide targets, 23rd IADR Annual Scientific Meeting . 2009. |
| Tsang P.C.S., Shibu T. and Perera R.A.P.M., Exoenzyme activities of Candida albicans in HIV-infecetd patients on HAART, 23rd IADR Annual Scientific Meeting . 2009. |
| Researcher : Pow EHN |
| Project Title: | Oral yeast profile of nasopharyngeal carcinoma patients after intensity-modulated radiotherapy |
| Investigator(s): | Pow EHN, McMillan AS, Leung WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To investigate oral colonization and phenotypic profile of oral yeasts in nasopharyngeal carcinoma (NPC) patients receiving conventional radiotherapy (CT) versus intensity-modulated radiotherapy (IMRT). |
| Project Title: | The impact of adjuvant chemotherapy on the quality of life of patients receiving radiotherapy for nasopharyngeal carcinoma |
| Investigator(s): | Pow EHN, McMillan AS, Kwong DLW, Wong MCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2006 |
| Completion Date: | 11/2009 |
| Abstract: |
| To compare oral health related quality of life in nasopharyngeal carcinoma (NPC) patients receiving radiotherapy (RT) versus radiotherapy plus chemotherapy (RTCH) |
| Project Title: | Detection of Epstein-Barr virus in saliva of patients receiving treatment for nasopharyngeal carcinoma |
| Investigator(s): | Pow EHN, Tsang PCS, Kwong DLW, McMillan AS |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2007 |
| Completion Date: | 02/2010 |
| Abstract: |
| To detect Epstein-Barr virus (EBV) DNA levels in saliva of patients receiving treatment for nasopharyngeal carcinoma (NPC) NPC is a cancer of epidermoid origin. It is rare in Caucasians but common in southern Chinese. In Hong Kong, NPC is the most common cancer in the head and neck region. The primary treatment of NPC is by radiotherapy. EBV is closely associated with NPC and it is present in virtually all of the neoplastic cells, suggesting that EBV is a marker of the neoplastic clone. With the advancement in DNA quantifying techniques such as real-time polymerase chain reaction (PCR), viral load can be accurately measured. Recent studies have shown that the level of EBV DNA in blood is proportional to the tumour burden in the NPC patients and it is shown to be a better method than conventional serologic tests in monitoring the disease. Studies have been reported using salivary EBV DNA level to monitor other medical or dental diseases such as HIV and periodontitis. However, no studies have been reported detecting the level of EBV DNA in saliva of the NPC patients. |
| Project Title: | Comparision of different extraction techniques and PCR assays for measuring salivary Epstein-Barr virus DNA levels in nasopharyngeal carcinoma patients |
| Investigator(s): | Pow EHN, Tsang PCS, Kwong DLW, McMillan AS |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2008 |
| Abstract: |
| To compare different extraction techniques and PCR assays for quantifying salivary Epstein-Barr virus (EBV) DNA levels in patients with nasopharyngeal carcinoma (NPC) NPC is a rare cancer in Caucasians but common in southern Chinese. In Hong Kong, NPC is the most common head and neck cancer. The primary treatment of NPC is by radiotherapy. EBV is closely associated with NPC and it is present in virtually all of the neoplastic cells, suggesting that EBV is a marker of the neoplastic clone. With the advancement in DNA quantifying techniques such as real-time polymerase chain reaction (PCR), viral load can be accurately measured. Recent studies have shown that the level of EBV DNA in blood is proportional to the tumour burden in the NPC patients and it is shown to be a better method than conventional serologic tests in monitoring the disease. Studies have been reported using salivary EBV DNA level to monitor other medical or dental diseases such as HIV and periodontitis. However, no studies have been reported the level of EBV DNA in saliva of the NPC patients. Our pilot data have shown that EBV DNA can be extracted and detected in saliva of NPC patients. Since only minute amount of saliva can be collected in such patients after cancer treatment, selection of extraction techniques and primer/probe sets should be optimized in order to quantity the salivary level of EBV DNA. |
| Project Title: | Second World Congress of the International Academy of Oral Oncology (IAOO 2009) A comparison of laboratory extraction techniques for the detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients |
| Investigator(s): | Pow EHN |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 07/2009 |
| Completion Date: | 07/2009 |
| Abstract: |
| N/A |
| Project Title: | Salivary anionic analysis of patients receiving radiotherapy for nasopharyngeal carcinoma |
| Investigator(s): | Pow EHN |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2009 |
| Abstract: |
| To assess changes of the major anions’ level in saliva of patients receiving radiotherapy for nasopharyngeal carcinoma (NPC) NPC is a cancer of epidermoid origin. It is rare in Caucasians but common in southern Chinese. In Hong Kong, NPC is the most common cancer in the head and neck region. The primary treatment of NPC is by radiotherapy. However, one of the most devastating complications following radiotherapy is “radiation caries”. In addition to the reduced salivary output, biochemical changes of the saliva following radiotherapy may also play an important role in causing this kind of intractable dental caries. However, limited prospective data regarding salivary ionic changes on irradiated patients were found and no such data on NPC patients are available. |
| List of Research Outputs |
| Au-Yeung N.K.H. and Pow E.H.N., Minimally invasive sinus-lift technique with simultaneous installation of a wide diameter implant: a case report, Hong Kong Dental Journal. 2009, 6: 98-102. |
| Leung K.C.M. and Pow E.H.N., Implant therapy for the medically compromised patients, In: Chen ZF, Research and clinical application of dental implant therapy. Beijing, People’s Military Medical Press, 2010, 83-91. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., A comparison of laboratory extraction techniques for the detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients., Oral Oncology. 2009, 3: 200. |
| Pow E.H.N., Advanced implant prosthodontics, Keynote lecture of Implant Prosthodontics and New Technology Course, Shanghai East Hospital, affiliated to Tongji University, Shanghai. 2010. |
| Pow E.H.N., Chen Z. and Leung K.C.M., Complications and their managing principles for dental implant therapy, In: Chen ZF, Research and clinical application of dental implant therapy. Beijing, People’s Military Medical Press, 2010, 235-246. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., Detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients: a comparison of two laboratory extraction techniques, Conjoint Scientific Sessions Faculty of Dentistry, The University of Hong Kong - Peking University School of Stomatology. 2009. |
| Pow E.H.N., Education and practice profile of implant dentistry in Hong Kong, Oral health and science seminar series, Faculty of Dentistry, The University of Hong Kong. 2010. |
| Pow E.H.N., How to minimize implant prosthodontic complications in your practice, Keynote lecture of Astra Tech Day, Tesco Dental Supplies Ltd, Hong Kong. 2010. |
| Pow E.H.N., How to prevent and manage implant prosthodontic complications, Keynote lecture of Joint Oral Implant Symposium, Changsha, Hunan. 2009. |
| Pow E.H.N., How to solve prosthodontic complications in implant dentistry, Keynote lecture of New developments in Oral Implant and Prosthodontics Symposium, Hangzhou, Zhejiang. 2009. |
| Pow E.H.N., Minimize prosthodontic complications of implant therapy, Joint Implant Symposium of Philippine Academy of Implant Dentistry and Oral & Maxillofacial Surgery, The University of Hong Kong. 2009. |
| Pow E.H.N., Oral Rehabilitation Teachers Training Workshop, Faculty of Dentistry, University of Hong Kong. 2010. |
| Pow E.H.N., Oral rehabilitation, dental implants and oral aesthetics, Once upon a tooth, Annual Oral Health Promotion Exhibition, Dental Society, HKUSU. 2009. |
| Pow E.H.N., Reviewer of the Editorial Review Board, Journal of Prosthodontics. 2010. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Student's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation 2009-2010. Hong Kong, 2009, 1-76. |
| Pow E.H.N., Surgical guide for implant therapy – Do you need it?, Joint Implant Symposium of Philippine Academy of Implant Dentistry and Oral & Maxillofacial Surgery, The University of Hong Kong. 2009. |
| Pow E.H.N., Botelho M.G., Chow T.W., Dias A.P.L.H., Dyson J.E., Leung K.C.M. and McMillan A.S., Teacher's manual to the BDS V prosthodontic clinical skills and technology course in oral rehabilitation, 2009-2010. Hong Kong, 2009, 1-89. |
| Pow E.H.N., Training workshop for additional examiners, The Dental Council of Hong Kong. 2010. |
| Tam C.K., Luk W.K., Pow E.H.N., Lo J. and Cheung L.K., Immediate Implant-Supported Auricular Prosthesis Using Surgical Navigation and CAD/CAM Technology - An In Vitro Trial, 9th Meeting of International Congress on Maxillofacial Rehabilitation. 2010. |
| Researcher : Rabie ABM |
| Project Title: | 79th European Orthodontic Society Congress Advantages of Early Treatment of Pseudo Class III |
| Investigator(s): | Rabie ABM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2003 |
| Abstract: |
| N/A |
| Project Title: | A novel treatment for Skeletal Class II malocclusion in young adults - a Bone Borne Mandibular Distractor |
| Investigator(s): | Rabie ABM, Cheung LK, Wong RWK, Dyson JE |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 10/2006 |
| Completion Date: | 09/2009 |
| Abstract: |
| (1) Enhancing the reactivation of condylar growth in young adults and late adolescents based on findings in experimental and clinical research. We aim to increase the skeletal component of the correction by avoiding the proclination of the lower incisors that occurs with the Herbst appliance. This reduces the overjet, the amount of advancement and skeletal correction. (2) The forces that this distractor will be exposed to in situ and hence the requisite bone plate configurations, screw length and diameter will be investigated. (3) The question of how to evaluate the response to treatment will also be addressed. In addition to traditional radiographic methods we will use new developments in 3D Magnetic Resonance Imaging. |
| Project Title: | Inhibit mandibular condylar growth with AAV-Vastatin |
| Investigator(s): | Rabie ABM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2008 |
| Completion Date: | 12/2009 |
| Abstract: |
| To inhibit mandibular condylar growth with AAV-Vastatin, to further investigate the role of anti-angiogenesis in the mandibular condylar growth. |
| List of Research Outputs |
| Al Khal H.M., Rabie A.B.M. and Wong R.W.K., Orthodontic tooth movement of total buccally blocked-out canine: a case report, Cases Journal 2009. 2009, 2: 7245. |
| Albaker B., Wong R.W.K. and Rabie A.B.M., Dentoskeletal effects of Herbst Appliance treatment among different skeletal maturity groups, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p27 (HP001). |
| Almoqubel A., Wong R.W.K., Zuo P. and Rabie A.B.M., Poster Presentations: Stem Cell Differentiation into Angiogenesis Lineage, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Anderson I.M., Rabie A.B.M. and Wong R.W.K., Poster Presentations: Early Treatment of Pseudo Class III Malocclusion – A 10-Year Follow-Up Study, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Cendekiawan T., Wong R.W.K. and Rabie A.B.M., Relationships Between Cranial Base Synchondroses and Craniofacial Development: A Review, The Open Anatomy Journal 2010. 2010, 2: 67-75. |
| Chaiyongsirisern A., Rabie A.B.M. and Wong R.W.K., Treatment effects and long-term stability of adult Class II patients treated with the Stepwise Advancement Herbst appliance, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p27 (HP004). |
| Chen K., Rabie A.B.M. and Wong R.W.K., Effects of LEPR fa/fa Mutation on Mandible Dimension and Their Impact and Diagnosis and Treatment of Mandibular Retrognathism, 1st Hong Kong International Dental Expo And Symposium (HKIDEAS) – Hong Kong Convention and Exhibition Centre, 18-20 June, 2010. |
| Chen K., Wong R.W.K. and Rabie A.B.M., Genetic predisposition to skeletal class II malocclusion in a Southern Chinese population, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p142 (SP441). |
| Chen K., Wong R.W.K. and Rabie A.B.M., Poster Presentation: LEPR is a Predisposing Factor of Mandibular Retrognathism; A SNP Association and Animal Study, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. |
| Chen K., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Polymorphisms of LEPR Gene in Mandibular Retrognathism, The 7th International Orthodontic Congress (IOC) World Federation of Orthodontists, Sydney – Australia, 6-9 February, 2010. |
| Chen K., Wong R.W.K. and Rabie A.B.M., Title: LEPR is a Predisposing Factor of Mandibular Retrognathism; A SNP Association and Animal Study , GSK Outstanding Oral Presentation (Junior). Awarded by the Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. 2009. |
| Chin A., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Medicinal Herb Salvia Miltiorrhiza on Osteoblastic Cell Line of MC3T3-E1, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Chin A., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Salvia Miltiorrhiza on Osteoblastic Cell Line of MC3T3-E1, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Geoghegan F., Wong R.W.K. and Rabie A.B.M., Inhibitory Effect of Quercetin on Periodontal Pathogens in vitro, Phytotherapy Research (online in Wiley InterScience). 2009. |
| Geoghegan F., Tsui V., Wong R.W.K. and Rabie A.B.M., The effects of orthodontic intrusion utilising two novel osteogenic compounds on periodontal bone levels, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June,. 2009, p83 (SP176). |
| Gu M., Rabie A.B.M. and Wong R.W.K., 邊缘骨性III類錯合手述與非手述效果的對比分析, Journal of Oral Science Research. 2009, 25 No.5: 611-614. |
| Gu M., Rabie A.B.M. and Wong R.W.K., 骨性III類錯合手述與非手述邊緣病例的顱面特征分析, 廣東牙病防治, 2009年12月. 2010, Vol 17, No.12: 571-573. |
| Hamza E., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Effect of Autotransplantation on Bone Regeneration in Orthodontic Patients, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Lei W.Y., Rabie A.B.M. and Wong R.W.K., Repair of a defect following the removal of an impacted maxillary canine by orthodontic tooth movement: a case report, The Cases Journal 2010. 2010, 3: 62. |
| Leung F.Y.C., Rabie A.B.M. and Wong R.W.K., Osteoporosis, Osteonecrosis and Orthodontics, World Journal of Orthodontics . 2009, 10: 261-271. |
| Li L., Wong R.W.K. and Rabie A.B.M., Oral Presentation: An anti-inflammatory and analgesic ointment for the relief of temporomandibular joint and masticatory muscle pain , 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p15 (OP024). |
| Li L.C.F., Wong R.W.K. and Rabie A.B.M., Clinical Effect of a Topical Herbal Ointment on Pain in Temporomandibular Disorders: A Randomized Placebo-controlled Trial, Journal of Alternative and Complementary Medicine 2009. 2009, 15 No.12: 1311-1317. |
| Li L.C.F., Rabie A.B.M. and Wong R.W.K., Management of Severe Class II Malocclusion with Sequential Fixed Functional and Orthodontic Appliances: A Case for MOrhRCSEd Examination, Singapore Dental Journal. 2010, 31 No.1: 30-35. |
| Li Q.F., Wong R.W.K. and Rabie A.B.M., Gene therapy: an alternative strategy for correction of severe craniofacial deformities, Hong Kong Dental Journal. 2010, 7: 8-13. |
| MacLaine J., Rabie A.B.M., Blechman A. and Wong R.W.K., Does orthodontic tooth movement cause an elevation in the systemic inflammatory markers? , 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p151 (SP476). |
| O'Connor D.J., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Resveratrol Inhibits Periodontal Pathogens In Vitro, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Porbunderwalla R., Wong R.W.K. and Rabie A.B.M., Tensile stress on the expression of Sox 9 and subsequent release of Type II collagen in a synchondrosis, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p28 (HP006). |
| Rabie A.B.M., Leung F.Y.C., Wong R.W.K. and Yang Y., A Bag of Tricks: Varying Timing of Extraction Facilitates Midline Correction, The Philippine Journal of Orthodontics. 2009, 8: 1-5. |
| Rabie A.B.M. and Yang Y., Perio-ortho conjoint treatment of periodontally compromised patients, Chinese Journal of Orthodontics. 2009, 16: 181-183. |
| Rizk A...E.L...S., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Transfection of Dental Pulp Stem Cells Using Electro-poration , The 7th International Orthodontic Congress (IOC) World Federation of Orthodontists, Sydney – Australia, 6-9 February, 2010. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of Vascular Endothelial Growth Factor (VEGF) on Osteoblastic Cells in vitro, Houston Poster Award - the 86th Congress of the European Orthodontic Society, Portoroz, Slovenia, June 15-19, 2010. 2010. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Vascular Endothelial Growth Factor (VEGF) on Osteoblastic Cell vitro, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Vascular Endothelial Growth Factor on MC3T3-E1 Cell Line, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Thongdee P., Rabie A.B.M. and Wong R.W.K., Poster Presentations: Use of AN ER, CR:YSGG, Waterlase Laser in Orthodontic Patients with Gingival Overgrowth, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Tsui V.W.K., Alkhal H.A., Hou H.M., Wong R.W.K. and Rabie A.B.M., The modified two-by-one fixed orthodontic appliance for bodily movement of canine: a case report, Cases Journal 2009. 2009, 2: 211. |
| Veerawattanatigul A., Wong R.W.K. and Rabie A.B.M., Poster Presentations: The Quercetin Effect on SOX9 and Collagen II Expressions in Spheno-Occipital Synchondroses, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Wangwarunyoo K., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Effect of Quercetin on Expression of BMP2 and PTHRP in Mice Spheno-Occipital Synchondroses, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Wong R.W.K. and Rabie A.B.M., Effect of Buguzhi (Psoralea corylifolia fruit) Extract on Bone Formation, Phytotherapy Research (online in Wiley InterScience). 2009. |
| Wong R.W.K. and Rabie A.B.M., Effect of Genistin on Bone Formation, Frontiers in Bioscience . 2010, E2: 764-770. |
| Wong R.W.K. and Rabie A.B.M., Grapefruit Flavonoids: naringin and naringinin, Flavonoids: Biosynthesis, Biological Effects and Dietary Sources. Nova Science Publishers, Inc. Nova publishers, 2009. |
| Wong R.W.K. and Rabie A.B.M., Oral Presentation: In vivo and in vitro effects of phytoestrogen Genistin on Bone Formation, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. 2009. |
| Wong R.W.K. and Rabie A.B.M., Oral Presentation: In vivo and in vitro effects of phytoestrogen Genistin on Bone Formation, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. |
| Wong R.W.K. and Rabie A.B.M., Phytochemicals for Bone Regeneration, Biomaterials for Bone Regenerative Medicine – Trans Tech Publications Inc. Materials Science Foundations (monograph series) . 2010, 62: 81-106. |
| Wong R.W.K., Alkhal H.A. and Rabie A.B.M., Use of cervical vertebral maturation to determine skeletal age, American Journal of Orthodontics & Dentofacial Orthopedics. 2009, 136: 484.e1-484.e6. |
| Wu A., McGrath C.P.J., Wong R.W.K., Wiechmann D. and Rabie A.B.M., Pain and daily oral impact and satisfaction of patients treated with labial and lingual fixed appliances, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p18 (OP033). |
| Xue F., Rabie A.B.M. and Wong R.W.K., Poster Presentation: Association analyses of chromosome 1p36 SNPs identify new positional candidate genes for mandibular prognathism, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. |
| Yang Y., Chin A., Tan Y.Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of VEGF and medicinal herb SM on bone cells, Honourable Mention Award of poster presentation at the 1st Hong Kong International Dental Expo and Symposium (HKIDEAS), Hong Kong, 18-20 June 2010. 2010. |
| Yang Y., Yang Y., Li X.T., Cui L., Fu M.K., Rabie A.B.M. and Zhang D., Functional analysis of core binding factor a1 and its relationship with related genes expressed by human periodontal ligament cells exposed to mechanical stress., European Journal of Orthodontics. 2010, Epub ahead of print. |
| Yang Y., Tan Y.Y., Chin A., Wong R.W.K. and Rabie A.B.M., Oral Presentation: Effects of Vascular Endothelial Growth Factor and Danshen on Osteoblastic Cell Activities, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Yang Y., Tan Y.Y., Chin A., Chai L., Wong R.W.K. and Rabie A.B.M., Oral Presentation: Effects of Vascular Endothelial Growth Factor (VEGF) and Medicinal Herb Salvia Miltiorrhiza (SM) on Osteoblastic Cell Activities, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Yang Y., Tan Y.Y., Chin A., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of VEGF and Medicinal Herb SM on bone cells, 1st Hong Kong International Dental Expo And Symposium (HKIDEAS) – Hong Kong Convention and Exhibition Centre, 18-20 June. 2010. |
| Yugaraj P., Zuo P., Rabie A.B.M. and Wong R.W.K., Poster Presentations: In Vitro Differentiation of Dental Pulp Stem Cells into the Chondrogenic Route, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Zhou W., Rabie A.B.M., Wong R.W.K. and Tang B., Nanocoating of Montmorillonite/Mg-β-Tricalcium Phosphate on Orthodontic Titanium Miniscrews, 3rd IEEE International NanoElectronics Conference (INEC). 2010, BC211. |
| Zuo P. and Rabie A.B.M., Novel method of cell-free in vitro synthesis of the human fibroblast growth factor 1 gene, Journal of Biomedicine and Biotechnology. 2010. |
| Researcher : Sam K |
| Project Title: | 79th Congress of the European Orthodontic Society Oral Appliance in Obstructive Sleep Apnea - the Efficacy and Predictive Factors |
| Investigator(s): | Sam K |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2003 |
| Abstract: |
| N/A |
| Researcher : Samaranayake LP |
| Project Title: | 79th General Session and Exhibition of the International Association for Dental Research Turbidometric Evaluation of Polyene-azole Antagonism in C. Albicans |
| Investigator(s): | Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2001 |
| Abstract: |
| N/A |
| Project Title: | Candida biofilms: molecular mechanisms and clinical implications |
| Investigator(s): | Samaranayake LP, Samaranayake YH, Jin LJ, Wang Y |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 12/2006 |
| Abstract: |
| To compare the gene and protein expression, relevant to its virulent attributes, in sessile and palnktonic phase Candida on an inert surface and an epithelial surface; to evaluate the biofilm-associated drug resistance of C. albicans to three representative, commonly used, major antifungal agents: amphotericin B (polyene group), fluconazole (azole group) and capsofungin (echinocandin group); to characterize the effects of three innate mucosal defense constituents: lactoferrin, histatins and defensins on Candida biofilms. |
| Project Title: | Biomarkers in proteomic profiles of Candida biofilms during antifungal exposure |
| Investigator(s): | Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2007 |
| Abstract: |
| Candida species are the commonest agents of oropharyngeal and systemic fungal infections both in health and disease. Candidiasis is the 4th leading cause of nosocomial infection in US, even beyond some common bacterial infections (Banerjee et al., 1991; Edmond et al., 1999). Furthermore, candidiasis is the most common fungal infection in both child and adult HIV/AIDS patients and in solid organ transplant (Patterson, 1999; Hagerty et al., 2003). Moreover, mortality rates among patients reported to be as high as 40% to 60% (Samaranayake et al, 2002; Wenzel and Gennings, 2005). Biofilms play a pivotal role in modern medicine. It is reported that at least 65% of all microbial infection are related to biofilm mode of growth (Potera, 1999). A vast majority of Candida infections are also related to its biofilm phase (i.e. attached to surfaces) as opposed to free-living or planktonic phase. Candida biofilms have been observed in most if not all medical devices in current use such as stents, shunts prosthesis (dentures, voice, heart valve, Knee, laryngeal devices etc), implants (lens, breasts, etc) endotracheal tubes, pacemakers, and various types of catheters (Ramage et al., 2006). These Candida biofilms are difficult to eradicate because of their very high antifungal resistance which can as high as 2000 times (Chandra et al., 2001;Ramage et al., 2001b).Thus, Candida biofilm associated infection followed many clinical and economical consequences. Understanding of mechanism underlying Candida biofilm development and regulation is a current challenge in the medical field. In our previous study of proteomics of Candida biofilms, we found biomarker sphingolipid base sensory protein (Pil1p) is highly expressed in the biofilm proteome compared to planktonic proteome. Interestingly, this protein possess an echinocandin binding domain. Echinocandin class of drugs such as caspofungin and micofungin have been shown to be more effective for Candida biofilms than conventional azole antifungals (Kuhn et al., 2002; Bachmann et al., 2002). Since, pil1p carries the echinocandin binding domain; we postulate that caspofungin is able to eradicate the Candida biofilms than conventional azole antifungals. Furthermore, caspofungin induced proteomic profiles will provide us valuable clues of the mechanism of action of this new antifungal against Candida biofilms. Therefore the aim of this study is, to evaluate the antifungal susceptibility to caspofungin against two Candida species namely C. albicans and C. glabrata, and then evaluate the proteomic changes associated with caspofungin. Biofilm formation is a major virulence attribute of Candida species. Especially, now it is widely evident that biofilm mode possesses higher antifungal susceptibility compared to planktonic mode (Ramage et al., 2001). Most of the traditional class of antifungals such as polyenes and azoles are inefficient against Candida biofilm related infections (Ref). In particular considered the immunocompromised host populations such as HIV/AIDS, transplant patients and patients on chemotherapy, Candida biofilms are related to the high mortality rate (Ref). Newer antifungal such as echinocandins have shown better results in recent studies (Ref). Interestingly, in our previous work we found the protein biomarkers Pil1p is highly expressed in biofilm mode of Candida compared to planktonic phase. Therefore we speculate this protein modulate the binding of echinocandin, thus can effectively eliminate the pathogenic fungi. References Bachmann,SP, VandeWalle,K, Ramage,G, Patterson,TF, Wickes,BL, Graybill,JR et al. (2002). In vitro activity of caspofungin against Candida albicans biofilms. Antimicrobial Agents and Chemotherapy 46(11):3591-3596. Banerjee,SN, Emori,TG, Culver,DH, Gaynes,RP, Jarvis,WR, Horan,T et al. (1991). Secular Trends in Nosocomial Primary Blood-Stream Infections in the United-States, 1980-1989. American Journal of Medicine 91:S86-S89. Chandra,J, Kuhn,DM, Mukherjee,PK, Hoyer,LL, McCormick,T, and Ghannoum,MA (2001). Biofilm formation by the fungal pathogen Candida albicans: Development, architecture, and drug resistance. Journal of Bacteriology 183(18):5385-5394. Edmond,MB, Wallace,SE, McClish,DK, Pfaller,MA, Jones,RN, and Wenzel,RP (1999). Nosocomial bloodstream infections in United States hospitals: A three-year analysis. Clinical Infectious Diseases 29(2):239-244. Kuhn,DM, George,T, Chandra,J, Mukherjee,PK, and Ghannoum,MA (2002). Antifungal susceptibility of Candida biofilms: Unique efficacy of amphotericin B lipid formulations and echinocandins. Antimicrobial Agents and Chemotherapy 46(6):1773-1780. Patterson,TF (1999). Approaches to fungal diagnosis in transplantation. Transpl Infect Dis 1(4):262-272. Potera,C (1999). Microbiology - Forging a link between biofilms and disease. Science 283(5409):1837-+. Ramage,G, Martinez,JP, and Lopez-Ribot,JL (2006). Candida biofilms on implanted biomaterials: a clinically significant problem. Fems Yeast Research 6(7):979-986. Ramage,G, Vande Walle,K, Wickes,BL, and Lopez-Ribot,JL (2001). Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrobial Agents and Chemotherapy 45(9):2475-2479. Samaranayake,LP, Fidel,PL, Naglik,JR, Sweet,SP, Teanpaisan,R, Coogan,MM et al. (2002). Fungal infections associated with HIV infection. Oral Diseases 8:151-160. |
| Project Title: | Molecular mechanisms regulating higher antifungal resistance of Candida krusei biofilms |
| Investigator(s): | Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2008 |
| Abstract: |
| Candida species are the commonest agents of oropharyngeal and systemic fungal infections. Candidiasis is the 4th leading cause of nosocomial infection in US, even beyond some common bacterial infections (Banerjee et al., 1991;Edmond et al., 1999). Biofilms play a pivotal role in infectious diseases. It is reported that at least 65% of all microbial infection are related to biofilm mode of growth (Potera, 1999). A vast majority of Candida infections are also related to its biofilm phase (i.e. surface attached ) as opposed to free-living or planktonic phase. Candida biofilms have been observed in most if not all medical devices in current use such as stents, shunts prosthesis (dentures, voice, heart valve, Knee, laryngeal devices etc), implants (lens, breasts, etc) endotracheal tubes, pacemakers, and various types of catheters (Ramage et al., 2006). These Candida biofilms are difficult to eradicate because of their very high antifungal resistance which can be as high as 2000 times the normal(Seneviratne et al., 2008a; Chandra et al., 2001).Thus, Candida biofilm associated infection is related to many clinical and economic consequences. Thus understanding of mechanism underlying Candida biofilm development and regulation is a challenge. Hence the bjectives of this study are: 1. To investigate the differential proteomics expression of C. krusei planktonic and biofilm mode 2. To relate the findings from above to the antifungal susceptibility of planktonic and biofilm modes C. krusei |
| Project Title: | Research Output Prize |
| Investigator(s): | Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Research Output Prize (in Faculty) |
| Start Date: | 12/2009 |
| Abstract: |
| To identify and recognize the best research outputs in different faculties. |
| Project Title: | Identification of Immuno-proteome of Candida albicans using a murine candidiasis model |
| Investigator(s): | Samaranayake LP, Seneviratne CJ, Wang Y |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 05/2010 |
| Abstract: |
| Objectives of the research proposal Key issues Candida is the most important fugal pathogen of humans, and it causes a variety of afflictions ranging from superficial mucosal infections to sometimes life-threatening systemic mycoses (Samaranayake & MacFarlane, 1990 ). Candida species are the fourth leading cause of nosocomial bloodstream infections in the USA (Wenzel & Gennings, 2005). Alarmingly, it was recently reported that hospital associated mortality in Candida blood infection (candidemia) in Hong Kong could be as high as 78% (Yap et al., 2009). Given the growing worldwide population of immunocompromised individuals, such as patients with neutropenia, people living with HIV/AIDS, organ transplant recipients, and patients receiving chemotherapy as cancer treatment, the incidence of candidemia is expected to continue rising. Hence, a thorough understanding of this yeast’s molecular mechanisms of infection, as well as of host immune responses, is urgently needed. Problems addressed Although a number of workers have investigated the pathophysiological mechanisms of in vivo host-Candida interactions, the exact mechanism by which Candida exerts its virulence and corrupts and escapes the host defenses is poorly understood. Furthermore, there are only sparse data available on the host immune response to well-recognized virulent features of Candida, such as formation of hyphae. For example, Candida hyphal mutants are less virulent in a mouse model (Lo et al., 1997). However, systematic and comprehensive studies of the well-characterized C. albicans SC 5314 wild type (WT) strain and its hyphal mutants are lacking. Such studies will provide greater insights into the fundamental pathophysiology of host-Candida interactions during candidemia and systemic candidiasis, thereby providing clues to the development of effective interventional and prophylactic approaches, such as vaccines, to eradicate this common infection. Objectives: To compare the pathophysiological sequelae of a wild type C. albicans and its hyphal mutant in a systemic candidiasis mouse model To identify the immunoreactive component/s of the proteomes of the wild type and hyphal mutant of Candida in a systemic candidiasis mouse model |
| List of Research Outputs |
| Ajonuma L.C., Lu Q., Cheung B.P.K., Leung W.K., Samaranayake L.P. and Jin L.J., Expression and localization of cystic fibrosis transmembrane conductance regulator in human gingiva, Cell Biology International. 2010, 34: 147-152. |
| Baboni F.B., Barp D., Izidoro A.C., Samaranayake L.P. and Rosa E.A., Enhancement of Candida albicans Virulence After Exposition to Cigarette Minstream Smoke, Mycopathologia. 2009, 168: 227-235. |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development, BMC Microbiology. 2010, 10: 125 (pages 1-9). |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation, Journal of Medical Microbiology. 2009, 58: 1623-1631. |
| Bridges S.M., Corbet E.F., Samaranayake L.P., Dyson J.E., Leung W.K. and McGrath C.P.J., Improving programme evaluations: The effect of curriculum review, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 37. |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Antimicrobial activity of Prunes extract on Streptococcus mutans, Second Prize of E-poster Competition for Postgraduate Students. Awarded by IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. 2010. |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Poster Presentation: Antimicrobial activity of Prunes extract on Streptococcus mutans, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Gao X., Seneviratne C.J., Lo E.C.M., Chu C.H. and Samaranayake L.P., Novel and conventional assays for assessing risk of dental caries, In: Programme book of Health Research Symposium 2010: Improving Health and Recognising Excellence. 2010, Ab56: p75. |
| Hibino K., Wong R.W.K., Hagg E.U.O. and Samaranayake L.P., The effects of orthodontic appliances on Candida in the human mouth, The International Journal of Paediatric Dentistry . 2009, 19: 301-308. |
| Hibino K., Samaranayake L.P., Hagg E.U.O., Wong R.W.K. and Lee W., The role of salivary factors in persistent oral carriage of Candida in orthodontic patients, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p151 (SP475). |
| Jin L.J., Lu Q., Darveau R.P., Samaranayake L.P. and Wang C.Y., P. gingivalis LPS modulates hBD-2 expression through TLR2 and 4, Journal of Dental Research. 2009, 88 (Spec Iss B): 749 (PAPF/APR). |
| Kang K., Wong K.S., Seneviratne C.J., Samaranayake L.P. and Fong W.P., In vitro synergistic effects of metergoline and antifungal agents against Candida krusei, 2009. |
| Lu Q., Darveau R.P., Samaranayake L.P., Wang C.Y. and Jin L.J., Differential modulation of human b-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- and penta-acylated lipid A structures, Innate Immunity. 2009, 15: 325-335. |
| Parahitiyawa N.B., Scully C., Leung W.K., Yam W.C., Jin L.J. and Samaranayake L.P., Exploring the oral bacterial flora: current status and future directions, Oral Diseases. 2010, 16: 136-145. |
| Perera R.A.P.M., Tsang P.C.S., Chan K.H., Lee M.P., Li P., Miller C.S. and Samaranayake L.P., Cytokine Interactome Alterations Promote Oropharyngeal Epstien-barr Virus (EBV) Replication During HIV Infection, Gene Expression Omnibus (GEO) Series GSE21339. 2010. |
| Perera R.A.P.M., Tsang P.C.S., McMillan A.S., Miller C.S., Lee M.P., Li P. and Samaranayake L.P., Oral chemokine interactome unfolds potential anti-HIV mucosal microbicide targets, 23rd IADR Annual Scientific Meeting . 2009. |
| Samaranayake L.P., Advisory Professor, Shanghai Jiao Tong University, School of Medicine. China, 2010. |
| Samaranayake L.P., Associate Editor, Mycopathologia. 2010. |
| Samaranayake L.P., Distinguished Scientist Award in Oral Medicine and Pathology, International Association for Dental Research. 2010. |
| Samaranayake L.P., Editor-in-Chief, Journal of Investigative and Clinical Dentistry. 2010. |
| Samaranayake L.P., Editorial Advisory Boards , Journal of Dental Education. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, Archives of Oral Biology. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, DPR Europe. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, Hong Kong Dental Journal. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, Indian Journal of Dental Research. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, International Journal of Dentistry. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, Japanese Journal of oral Biosciences. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, Mycoses. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, Saudi Dental Journal. 2010. |
| Samaranayake L.P., Editorial Advisory Boards, Sri Lanka Dental Journal. 2010. |
| Samaranayake L.P., Honorary Professor, Eastman Dental Institute, University College. London, UK, 2010. |
| Samaranayake L.P., Member of the International Advisory Panel, Oman Dental College. Oman, 2010. |
| Samaranayake L.P., Research Output Prize, Faculty of Dentistry (as a co-investigator), 2010. |
| Samaranayake L.P., Tam Wah-Ching Endowed Professor in Dental Science , 2010. |
| Samaranayake Y.H., Yau J.Y.Y., THEIN Z.M., Jayatilake J.A.M.S., Yeung S.K.W. and Samaranayake L.P., The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its imact on the colonization traits of Candida glabrata, Medical Mycology. 2010, 1-10. |
| Seneviratne C.J., Samaranayake L.P., Wong R.W.K. and Hagg E.U.O., Antimicrobial activity of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study.Int J Oral Maxillofac Surg. 2010 Apr 23, 2010. |
| Seneviratne C.J., Silva W.J., Jin L.J., Samaranayake Y.H. and Samaranayake L.P., Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms, Archives of Oral Biology. 2009, 54: 1052-1060. |
| Seneviratne C.J., Wang Y., Jin L.J., Abiko Y. and Samaranayake L.P., Proteomics of drug resistance in Candida glabrata biofilms, Proteomics 2010 Apr;10(7):1444-54., 2010. |
| Seneviratne C.J., Wang Y., Jin L.J., Abiko Y., Watamoto T. and Samaranayake L.P., Shotgun proteomics elucidates the regulatory pathway of Candida biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 254 (PAPF/APR). |
| So C.W., Tsang W.K., Lo P.C., Seneviratne C.J., Samaranayake L.P. and Fong W.P., Photodynamic inactivation of Candida albicans by BAM-SiPc, Mycoses. 2010, 53: 215-220. |
| Tsang P.C.S. and Samaranayake L.P., Immune reconstitution inflammatory syndrome after highly active antiretroviral therapy: a review, Oral Diseases. 2010, 16: 248-56. |
| Watamoto T., Samaranayake L.P., Egusa H., Yatani H., Samaranayake Y.H. and Seneviratne C.J., Susceptibility of Candida albicans filamentation-defective mutants to clinical biocides (corresponding author), The Journal of Hospital Infection. 2010, 74(2): 189-91. |
| Wong R.W.K., Hagg E.U.O., Samaranayake L.P., Yuen M.K.Z., Seneviratne C.J. and Kao R., Antimicrobial activities of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study, International Journal of Oral & Maxillofacial Surgery. 2010, 39: 599-605. |
| Yuen K., Wong R.W.K., Hagg E.U.O. and Samaranayake L.P., Antimicrobial activities of forty traditional Chinese medicines on oral bacteria: A experimental study, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p152 (SP480). |
| Researcher : Samaranayake YH |
| Project Title: | 84th General Session and Exhibition of the International Association for Dental Research (IADR) Synergistic Effect of Lysozyme and Amphotericin B on Candida Biofilms |
| Investigator(s): | Samaranayake YH |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2006 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Samaranayake Y.H., Yau J.Y.Y., THEIN Z.M., Jayatilake J.A.M.S., Yeung S.K.W. and Samaranayake L.P., The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its imact on the colonization traits of Candida glabrata, Medical Mycology. 2010, 1-10. |
| Seneviratne C.J., Silva W.J., Jin L.J., Samaranayake Y.H. and Samaranayake L.P., Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms, Archives of Oral Biology. 2009, 54: 1052-1060. |
| Watamoto T., Samaranayake L.P., Egusa H., Yatani H., Samaranayake Y.H. and Seneviratne C.J., Susceptibility of Candida albicans filamentation-defective mutants to clinical biocides (corresponding author), The Journal of Hospital Infection. 2010, 74(2): 189-91. |
| Researcher : Samman N |
| List of Research Outputs |
| Choi W.W.S., Lee S..., McGrath C.P.J. and Samman N., Change in quality of life after combined orthodontic-surgical treatment of dentofacial deformities, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2010, 109 (1): 46-51. |
| Chua H.D.P., Whitehill T.L., Samman N. and Cheung L.K., Maxillary distraction versus orthognathic surgery in cleft lip and palate patients: Effects on speech and velopharyngeal function , Int J Oral Maxillofac Surg. 2010, 39: 633-40. |
| Samman N., Ameloblastoma: pathology, surgical management and controversies - Abstract Book, 16th International congress of Turkish association of oral and maxillofacial surgeons, 3-8 November 2009, Capadoccia, Turkey. 2009, 28-29. |
| Samman N., Ameloblastoma: pathology, surgical management and controversies, 16th International Congress of Turkish Association of Oral and Maxillofacial Surgeons, Capadoccia, Turkey. 2009. |
| Samman N., Association of Oral and Maxillofacial Surgeons of Singapore, 2009 Annual Meeting. 2009. |
| Samman N., Chairman (2009 - 2011), IAOMS Foundation. 2009. |
| Samman N., Challenges and Opportunities in an Oral and Maxillofacial Surgery Career, Fifteenth Annual C.J. Lyons Endowed Lecture Department of Oral and Maxillofacial Surgery University of Michigan Ann Arbor, Michigan, USA. 2010. |
| Samman N., Chalmers J Lyons Endowed Lectureship (2010), University of Michigan, Ann Arbor, Michigan, USA. 2010. |
| Samman N., Cleft Lip and Palate - Overview for Dentists, Oral Health Science Seminar - Prince Philip Dental Hospital, Hong Kong. 2010. |
| Samman N., Cleft surgery - ideals, priorities and evidence, 16th congress of the Latin American Association of Oral and Maxillofacial Surgeon (ALACIBU), Foz do Iguaçu - Brazil. 2009. |
| Samman N., Director, Cleft Lip and Palate Centre, University of Hong Kong. 2009. |
| Samman N., Examiner, Conjoint Intermediate Examination FCDSHK and Membership in Oral Surgery, RCS(Edin) since 1998, Hong Kong College of Dental Surgeons Committees. 2009. |
| Samman N., Examiner, Exit Assessment and Examination for oral and maxillofacial surgery (since 1999), Hong Kong College of Dental Surgeons Committees. 2009. |
| Samman N., External Affairs Officer (since 2008), Asian Association of Oral & Maxillofacial Surgeons. 2009. |
| Samman N., External Assessor (2006 - 2008, 2008 - 2010), University of Malaya. 2009. |
| Samman N., External Examiner (2010), University of Malaya. 2010. |
| Samman N., Globalisation in oral and maxillofacial surgery, IADR Asia Pacific Region Meeting, Wuhan, China. 2009. |
| Samman N., Guesst Professor, School of Stomatology, Peking University, Beijing, PR China. 2009. |
| Samman N., Guest Professor, School of Stomatology, Shanghai Jiaotong University, Ninth People's Hospital, Shanghai, PR China. 2009. |
| Samman N., HK West Cluster Medical Committee, Queen Mary Hospital Committees. 2009. |
| Samman N., Honorary Consultant and Chief of Service, Maxillofacial Unit, Department of Oral Maxillofacial & Dental Surgery, Queen Mary Hospital and Hong Kong West Cluster Hospitals, Hospital Authority of Hong Kong. 2009. |
| Samman N., Honorary Life Fellowship, American Association of Oral and Maxillofacial Surgeons, USA. 2009. |
| Samman N., Honorary Professorship (2009), School of Stomatology, Peking University, China. 2009. |
| Samman N., Honorary Visiting Professor in Surgery, Faculty of Dentistry, Mahidol University, Bangkok, Thailand. 2009. |
| Samman N., International training and scope of practice: how does the US compare with other industrialized nations, Oral and Maxillofacial Surgery Summit, Mount Hood, Oregon, USA. 2009. |
| Samman N., Member of Executive Committee (since 2000), Asian Association of Oral & Maxillofacial Surgeons. 2009. |
| Samman N., Member of the Editorial Board (since 1997), Chinese Journal of Oral and Maxillofacial Surgery. 2009. |
| Samman N., Member, Committee on Accreditation and Training in Oral and Maxillofacial Surgery (since 2001). , Hong Kong College of Dental Surgeons Committees. 2009. |
| Samman N., Operating Theatre Users Committee (since 1999), Queen Mary Hospital Committees, Hong Kong. 2009. |
| Samman N., Past President (2008 - 2010), Hong Kong Association of Oral and Maxillofacial Surgeons. 2009. |
| Samman N., President & Chairman, Board of Directors (2007-2009), International Association of Oral and maxillofacial Surgeons. 2009. |
| Samman N., Prevention and management of relapse after maxillary orthognathic surgery, 34th Annual Conference of the Association of Oral and Maxillofacial Surgeons of India and 1st Joint Conference with the British Association of Oral and Maxillofacial Surgeons, Cochin, India. 2009. |
| Samman N., Referee, European Journal of Oral Sciences; International Dental Journal; Hong Kong Medical Journal; Asian Journal of Surgery; Angle Orthodontist; Journal of Plastic Reconstructive and Aesthetic Surgery; Head and Neck; Clinical Nutrition. 2009. |
| Samman N., Section Chairman (Clinical Pathology) since 2001, International Journal of Oral and Maxillofacial Surgery. 2009. |
| Samman N., Speech after glossectomy - surgical implications, Conference on Advances in Stomatology 2010, Central South University, Shangsha, Hunan Province, China. 2010. |
| Samman N., Visiting Professor, Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Haad Yai, Thailand. 2009. |
| Xue S.A., Lam C., Whitehill T.L. and Samman N., Effects of Class III malocclusion on young adults' vocal tract development: A pilot study, The International Clinical Linguistics and Phonetics Association Conference, Oslo, 23 - 26 June 2010. |
| Xue S.A., Lam C., Whitehill T.L. and Samman N., Effects of Class III malocclusions on the vocal tract of young adults: a pilot study, 13th International Conference of Clinical Linguistics and Phonetics. Oslo, Norway. . 2010. |
| Researcher : Seneviratne CJ |
| Project Title: | 6th Joint BSPR/EBI Proteomics Meeting Shotgun Proteomics elucidates the regulatory pathway of Candida biofilms |
| Investigator(s): | Seneviratne CJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 07/2009 |
| Completion Date: | 07/2009 |
| Abstract: |
| N/A |
| Project Title: | Phosphoproteomics of Candida hyphal formation |
| Investigator(s): | Seneviratne CJ, Samaranayake LP, Wang Y, Jin LJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2009 |
| Abstract: |
| Key issues Candida is a ubiquitous commensal that often colonizes the oral cavity, gastrointestinal tract, vagina and skin of healthy individuals. Candida becomes pathogenic and causes candidal infection when the balance of the normal flora is disrupted or host immune defenses are compromised (Samaranayake & MacFarlane, 1990 ). Over the last decade, Candida infection has been increased dramatically due to the growing body of compromised host populations such as HIV/AIDS, organ transplant recipients and patients on chemotherapy (Becksague & Jarvis, 1993, Zaoutis et al., 2005). Candida infections range from superficial mucosal infections to deep seated systemic mycoses. Once Candida enters into the blood stream, it rapidly spreads into internal organs and invades tissue, causing fatal infection. Hence, mortality rate associated with Candida systemic infection has been reported to be as high as 50% in organ transplant recipients (Richardson, 2005, Wenzel & Gennings, 2005).Candida albicans is the most prevalent Candida species recovered from clinical samples. In general, four classes of antifungal drugs are used to manage fungal infections, namely polyenes, azoles, fluoropyrimidines and echinocandins. A rapid increase in the number of patients treated with these antifungals has led to the emergence of drug resistance Candida strains that have become a major clinical concern. (Ruhnke et al., 2008). Moreover, there are known side effects and toxicities of the antifungal drugs available to date (Ellis, 2002, Lewis, 2007). Therefore, there is a continuous search of novel drug targets which can specifically eradiate fungi with minimal effect to the host cells. Understanding of mechanism of Candida infection and role of its major virulent attribute will certainly facilitate the search of novel drug targets. Problems being addressed: One of the most important virulent factors of C. albicans is its ability to produce long filamentous forms known as hyphae (Kumamoto & Vinces, 2005). This morphogenic plasticity has long been considered to play a major role in its virulence. Hence, Candida hypha facilitates tissue invasion and infection. Candida hyphal formation is thought to be regulated by two major signal transuduction pathways; mitogen-activated protein kinase (MAPK) pathway and cAMP-dependent protein kinase A (PKA) pathway (Dhillon et al., 2003). In this regard, the transcriptional regulators Cph1p and Efg1p are the two major transcriptional regulators of MAPK and PKA pathways, respectively. These transcriptional factor genes EFG1 and CPH1 are positive regulators of hyphal morphogenesis, and deletion of either causes suppression of filamentous growth (Liu et al., 1994, Stoldt et al., 1997). Protein phosphorylation is a key reversible modification that regulates signaling networks (Preisinger et al., 2008). MAPK and PKA pathways perform sensing and transmitting of the response of cells to the environmental conditions by the sequential action of phosphorylation events (Bockmuhl & Ernst, 2001). Therefore, determining the phosphorylated proteins associated with these signaling pathways brings deeper understating of the molecular mechanism of such biological processes. Traditionally protein phosphorylation has been studied individually or by group of small number of proteins. However, recent advent in posphoproteomics technologies has opened a new avenue for researchers to identify and quantify the phosphorylation of proteome in global scale (de la Fuente van Bentem et al., 2008, Schreiber et al., 2008). Therefore, phosphoproteomics approaches can be used as very useful in elucidating phosphorylated proteins related to MAPK and PKA pathway in C. albicans. This in turn will show the proteins which mediate the signal transduction during Candida hyphal formation. However, there appear to be no study in the literature on Candida phosphoproteomics. In our previous study, we examined the phosphoproteomics of Candida biofilms (Seneviratne et al, unpublished data). In fact, C. albicans hyphal formation and biofilm formation has also been shown to be inter-related. Both of these biological processes are thought to be mediated through MAPK or PKA pathways. In aforementioned study, we used isobaric tags for relative and absolute quantification (iTRAQ) technology to elucidate the quantitative proteomics of Candida biofilms. As mentioned above, we also studied the phosphorylation pattern of identified proteins. It was found that Efg1 mutant proteome is dramatically different from C. albicans wild type SC5314 and key proteins related to PKA pathway were identified as potential biomarkers which regulate the Candida biofilm formation (Seneviratne et al, unpublished data). Therefore, in the present study we aim to elucidate the molecular mechanism of hyphal formation using C. albicans wild type SC5314 and Cph1 and Efg1 mutants by different quantitative proteomic approach. We further intent to study the phosphoproteomics of these pathways which will provide us a global view of the biologically active proteins during Candida hyphal formation. Once these data are available we will be able to cross-compare the phosphoproteins related to Candida biofilm formation and hyphal formation which will give us a clear understanding of these two virulent attribute and which proteins are actively participate in MAPK and PKA pathways during Candida infections . Purpose of this project: 1.To investigate the comparative proteomics of Candida hyphal formation regulated by MAPK and PKA pathways 2.To investigate the phosphorylated proteins related to MAPK and PKA pathways during Candida hyphal formation References Agrawal, G. K. & J. J. Thelen, (2005) Proteomics 5: 4684-4688. Becksague, C. M. & W. R. Jarvis, (1993) Journal of Infectious Diseases 167: 1247-1251. Blagoev, B., S. E. Ong, I. Kratchmarova & M. Mann, (2004) Nat Biotechnol 22: 1139-1145. Bockmuhl, D. P. & J. F. Ernst, (2001) Genetics 157: 1523-1530. de la Fuente van Bentem, S., W. I. Mentzen, A. de la Fuente & H. Hirt, (2008) Proteomics 8: 4453-4465. Dhillon, N. K., S. Sharma & G. K. Khuller, (2003) Crit Rev Microbiol 29: 259-275. Ellis, D., (2002) J Antimicrob Chemother 49 Suppl 1: 7-10. Gruhler, A., J. V. Olsen, S. Mohammed, P. Mortensen, N. J. Faergeman, M. Mann & O. N. Jensen, (2005) Mol Cell Proteomics 4: 310-327. Jayatilake, J. A., L. P. Samaranayake, Q. Lu & L. J. Jin, (2007) Oral Dis 13: 426-433. Kumamoto, C. A. & M. D. Vinces, (2005) Cell Microbiol 7: 1546-1554. Lewis, R. E., (2007) Curr Opin Pharmacol 7: 491-497. Liu, H., J. Kohler & G. R. Fink, (1994) Science 266: 1723-1726. Lu, Q., J. A. Jayatilake, L. P. Samaranayake & L. Jin, (2006) J Invest Dermatol 126: 2049-2056. Platt, M. D., A. M. Salicioni, D. F. Hunt & P. E. Visconti, (2009) . J Proteome Res 8: 1431-1440. Preisinger, C., A. von Kriegsheim, D. Matallanas & W. Kolch, (2008) Proteomics 8: 4402-4415. Richardson, M. D., (2005) J Antimicrob Chemother 56 Suppl 1: i5-i11. Ruhnke, M., K. Hartwig & G. Kofla, (2008) Clin Microbiol Infect 14 Suppl 4: 46-54. Samaranayake, L. P. & T. W. MacFarlane, (1990 ) Oral Candidosis Wright-Butterworth, London. Schreiber, T. B., N. Mausbacher, S. B. Breitkopf, K. Grundner-Culemann & H. Daub, (2008) Proteomics 8: 4416-4432. Seneviratne, C. J., Y. Wang, L. Jin, Y. Abiko & L. P. Samaranayake, (2008) Proteomics 8: 2936-2947. Stoldt, V. R., A. Sonneborn, C. E. Leuker & J. F. Ernst, (1997) EMBO J 16: 1982-1991. Wang, Y., L. Y. Xu, K. S. L. Lam, G. Lu, G. J. S. Cooper & A. M. Xu, (2006) Proteomics 6: 3862-3870. Watamoto, T., L. P. Samaranayake, J. A. Jayatilake, H. Egusa, H. Yatani & C. J. Seneviratne, (2009) Int J Antimicrob Agents. Wenzel, R. P. & C. Gennings, (2005) Clinical Infectious Diseases 41: S389-S393. Zaoutis, T. E., J. Argon, J. Chu, J. A. Berlin, T. J. Walsh & C. Feudtner, (2005) Clin Infect Dis 41: 1232-1239. |
| List of Research Outputs |
| Chu C.H., Mei L., Lo E.C.M., Seneviratne C.J. and Yip H.K., Effects of silver diamine fluoride on mono-species cariogenic biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 363 (PAPF/APR). |
| Gao X., Seneviratne C.J., Lo E.C.M., Chu C.H. and Samaranayake L.P., Novel and conventional assays for assessing risk of dental caries, In: Programme book of Health Research Symposium 2010: Improving Health and Recognising Excellence. 2010, Ab56: p75. |
| Kang K., Wong K.S., Seneviratne C.J., Samaranayake L.P. and Fong W.P., In vitro synergistic effects of metergoline and antifungal agents against Candida krusei, 2009. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Action of Silver Diamine Fluoride on Dentin Caries [Abstract], First Hong Kong International Dental Expo and Symposium; Hong Kong Dental Association. 2010, In Abstracts of papers: 84. |
| Mei L., Seneviratne C.J., Chu C.H. and Lo E.C.M., Effects of multi-species biofilm dentin caries-like lesions in artificial mouth, Journal of Dental Research. 2010, 89 (Spec Iss A): 1393 in AADR 39th Annual Meeting. |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., Effects of silver-diamine fluoride on caries-like lesion and cariogenic biofilm, Journal of Dental Research. 2009, 88 (Spec Iss B): 40 (PAPF/APR). |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., International Association for Dental Research / Unilever Hatton Travel Award for M.Lei (co-author), 23nd Annual Scientific Meeting, Southeast Asian Division of International Association for Dental Research 2009. . 2009. |
| Seneviratne C.J., Samaranayake L.P., Wong R.W.K. and Hagg E.U.O., Antimicrobial activity of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study.Int J Oral Maxillofac Surg. 2010 Apr 23, 2010. |
| Seneviratne C.J., Silva W.J., Jin L.J., Samaranayake Y.H. and Samaranayake L.P., Architectural analysis, viability assessment and growth kinetics of Candida albicans and Candida glabrata biofilms, Archives of Oral Biology. 2009, 54: 1052-1060. |
| Seneviratne C.J., Outstanding Research Postgraduate Student (2007-2008), The University of Hong Kong, The University of Hong Kong. 2009. |
| Seneviratne C.J., Plaque structure, formation and maturation, BDS I Resource lecture series, Faculty of Dentistry, HKU. 2010. |
| Seneviratne C.J., Wang Y., Jin L.J., Abiko Y. and Samaranayake L.P., Proteomics of drug resistance in Candida glabrata biofilms, Proteomics 2010 Apr;10(7):1444-54., 2010. |
| Seneviratne C.J., Wang Y., Jin L.J., Abiko Y., Watamoto T. and Samaranayake L.P., Shotgun proteomics elucidates the regulatory pathway of Candida biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 254 (PAPF/APR). |
| So C.W., Tsang W.K., Lo P.C., Seneviratne C.J., Samaranayake L.P. and Fong W.P., Photodynamic inactivation of Candida albicans by BAM-SiPc, Mycoses. 2010, 53: 215-220. |
| Watamoto T., Samaranayake L.P., Egusa H., Yatani H., Samaranayake Y.H. and Seneviratne C.J., Susceptibility of Candida albicans filamentation-defective mutants to clinical biocides (corresponding author), The Journal of Hospital Infection. 2010, 74(2): 189-91. |
| Researcher : Shi X |
| List of Research Outputs |
| Zheng L., Ma L., Shi X. and Cheung L.K., Comparison of distraction osteogenesis versus costochondral graft in reconstruction of tempormandibular joint condylectomy with disc preservation, J Oral Maxillofac Surg . 2010, In press. |
| Researcher : Siu SC |
| List of Research Outputs |
| Siu S.C., Chu F.C.S., Li T.K.L., Chow T.W. and Deng F., Imaging modalities for preoperative assessment in dental implant therapy: an overview, Hong Kong Dental Journal. 2010, 7(1): 23-30. |
| Researcher : Tam CK |
| List of Research Outputs |
| Tam C.K., Luk W.K., Pow E.H.N., Lo J. and Cheung L.K., Immediate Implant-Supported Auricular Prosthesis Using Surgical Navigation and CAD/CAM Technology - An In Vitro Trial, 9th Meeting of International Congress on Maxillofacial Rehabilitation. 2010. |
| Researcher : Tan H |
| List of Research Outputs |
| Tan H., Lo E.C.M., Luo Y., Dyson J.E. and Corbet E.F., Prevention of root caries in Chinese institutionalized elders, Journal of Dental Research. 2009, 88 (Spec Iss C): 129 (WCPD). |
| Researcher : Tang ATH |
| List of Research Outputs |
| Tang A.T.H. and Hagg E.U.O., Composite occlusal bite blocks bonded without liquid resin, 86th Congress of the European Orthodontic Society. 2010. |
| Tang A.T.H. and Hagg E.U.O., Lingual retainers bonded without liquid resin - a 5-year clinical study, The Second International Symposium on Surface and Interface of Biomaterials. 2010. |
| Tang A.T.H. and Hagg E.U.O., Survival analysis of occlusal bite blocks bonded without liquid resin, Annual Conference of International Association of Dental Research, Southeast Asian Division. 2009. |
| Researcher : Tay FRCM |
| List of Research Outputs |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R.C.M., Anti-microbial efficacy of 3.8% silver diamine fluoride and its effect on root dentin., Journal of Endodontics. 2010, 36:: 1026-1029. |
| Hiraishi N., Yiu C.K.Y., King N.M., Tagami J. and Tay F.R.C.M., Antimicrobial efficacy of 3.8% silver diamine fluoride and its effect on root dentin, Journal of Endodontics. 2010, 36(6): 1026-1029. |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R.C.M., Effect of chlorhexidine incorporation into a self-etching primer on dentine bond strength of a luting cement., Journal of Dentistry. 2010, 38: 496-502. |
| Kim J.R., Mai S., Carrilho M.R., Yiu C.K.Y., Pashley D.H. and Tay F.R.C.M., An all-in-one adhesive did not etch beyond hybrid layers. , Journal of Dental Research. 2010, 89: 482-487. |
| Kim Y.K., Yiu C.K.Y., Kim J.R., Gu L.S., Kim S.K., Weller R.N., Pashley D.H. and Tay F.R.C.M., Failure of a Glass Ionomer to Remineralize Apatite-depleted Dentin. , Journal of Dental Research. 2010, 89: 230-235. |
| Mai S., Kim Y., Kim J., Yiu C.K.Y., Ling J., Pashley D.H. and Tay F.R.C.M., In vitro remineralization of severely compromised bonded dentin., Journal of Dental Research . 2010, 89: 405-410. |
| Researcher : Tideman H |
| Project Title: | Functional reconstruction of the upper jaw |
| Investigator(s): | Tideman H, Luk WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 01/1992 |
| Abstract: |
| To restore the function of the upper jaw after ablative surgery with bone and implants. |
| Project Title: | Functional reconstruction of the lower jaw |
| Investigator(s): | Tideman H, Luk WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 01/1994 |
| Abstract: |
| To restore the function of the lower jaw after ablative surgery with bone and implants. |
| Researcher : Tong LSM |
| List of Research Outputs |
| Au A.H.O., Hui K.K.K., Lau E.W.S., Tong L.S.M. and Yiu C.K.Y., Guidelines on sedation in pediatric patients, Hong Kong Society of Pediatric Dentistry . 2009. |
| Researcher : Tsai JSJ |
| List of Research Outputs |
| King N.M., Tsai J.S.J. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part I: Anomalies in the permanent dentition, The Open Anthropology Journal. 2010, 3: 54-64. |
| King N.M., Tsai J.S.J. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part II: Traits in the permanent dentition , The Open Anthropology Journal. 2010, 3: 71-84. |
| Researcher : Tsang PCS |
| Project Title: | Clinical, microbiological and histopathological study of oral diseases in renal transplant patients in Hong Kong |
| Investigator(s): | Tsang PCS, Leung WK, Samaranayake LP, Chan AWK, Chan DTM, Chu FCS |
| Department: | Dental Faculty |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 03/2000 |
| Abstract: |
| To obtain baseline data on the oral health in a group of renal transplant patients; to compare the effects of cyclosporin A ad FK506 on oral tissues and oral mciroflora; to investigate the effects of oral health programme on the oral status of these patients, both clinically and microbiologically. |
| Project Title: | Epstein-Barr virus genotypes and their prevalence in oral samples of HIV-infected patients |
| Investigator(s): | Tsang PCS, McMillan AS, Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2007 |
| Completion Date: | 10/2009 |
| Abstract: |
| EBV is more prevalent in the oral cavity than any other viruses and it has been associated with many significant oral conditions. Its spread by saliva is high in HIV/AIDs patients and to date there is no definitive treatment for it. Therefore, it is important to investigate EBV in saliva as it may throw some light on the pathogenicity, reactivation and clinical manifestations of EBV in the oral cavity in HIV/AIDS patients. In the present study we plan to identify the strain variations of EBV in clinical oral samples in HIV/AIDS patients and to correlate it with patient socio-demographic data, CD4/ CD8 count, HIV viral load, any symptoms or active infection, presence of oral lesions and effect of HAART. These findings will be important for developing new strategies to inhibit EBV reactivation in HIV/AIDS population. Aims 1. To use non-invasive sampling techniques such as saliva, oral rinse and tongue scrapings to isolate EBV from a groups of Chinese HIV infected patients. 2. To determine the prevalence of strain variants of EBV in a group of Chinese HIV infected patients and of the control group and to compare the variation of EBV strains in HIV infected patients and healthy individuals. 3. To identify the risk factors associated with infection of EBV. Objectives 1. To detect the EBV strains in saliva, tongue brushings and in oral rinse in a group of Chinese HIV infected patients. 2. To identify the EBV strain genotypes and their prevalence in oral samples of HIV infected patients and healthy control group using standard molecular based techniques. 3. To correlate the strains of EBV with age, sex, CD4/ CD8 count, any symptoms or active infections, recent HAART and presence of oral lesions. |
| Project Title: | Epstein-barr virus and trasnsforming growth factor in HIV infected patients |
| Investigator(s): | Tsang PCS |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2008 |
| Abstract: |
| Earlier studies have demonstrated that EBV and its gene products can induce transforming growth factor (TGF-b) production and secretion from human cells and epithelial cells of the salivary glands and oropharyngeal tissue (Ahmad and Menezes, 1997; Cayrol and Flemington, 1995). The levels of TGF-b are significantly increased in the sera of patients with EBV-associated disease conditions (Xu et al., 1999). According to Nagata et al. (2004) they suggest TGF-b1 may play a role in EBV reactivation and they have found significantly elevated TGF-b1 levels in saliva in association with EBV. The cytokine, TGF-b1 is known to cause disruption of viral latency and stimulate EBV replication (Pi et al., 1987). The TGF-b family of cytokines is a group of closely related polypeptides of which TGF-b1 is the prototype. TGF-b is one of the most immunosuppressive substances found in the human body (Letterio and Roberts, 1998). It is produced by a wide variety of cells and tissues and plays an important role in cell differentiation, growth, matrix formation, and the regulation of immune and inflammatory responses (Letterio and Roberts, 1998). It may inhibit or stimulate cell growth, depending upon the cell type and culture conditions. TGF-b mediated signaling involves some unique second messengers and transcription factors called SMAD proteins. Defects in the TGF-b mediating signaling pathways have been causally linked to several human cancers (Kadin et al., 1994; Markowitz et al., 1995; Zhang et al., 1997). EBV is more prevalent in the oral cavity than any other viruses and it has been associated with many significant oral conditions. Its spread by saliva is high in HIV/AIDs patients and to date there is no definitive treatment for it. Therefore, it is important to investigate EBV and TGF-b1 in saliva as it may throw some light on the pathogenicity, reactivation and clinical manifestations of EBV in the oral cavity in HIV/AIDS patients. In the present study we plan to determine the levels of TGF-b in saliva in patients with HIV. These findings will be important for developing new strategies to inhibit EBV reactivation in HIV/AIDS population. |
| Project Title: | Effect of TGF- β1, vIL-10 in saliva of HIV patients on EBV lytic genes BZLF1 and BRLF1 expression |
| Investigator(s): | Tsang PCS, Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2009 |
| Abstract: |
| Epstein-Barr virus (EBV), a member of herpes virus group, is shed in significantly higher amounts in oropharynx of HIV infected individuals under highly active antiretroviral therapy (HAART)(Dehee et al., 2001; Griffin et al., 2008). Transforming growth factor (TGF)-β1 is one of the most immunosuppressive substances (Teicher, 2007) which is known to increase EBV replication by inducing EBV lytic promoters concentration dependently (Andersson, 1996; Nagata et al., 2004). HIV virus has shown to increase levels of TGF-β1 in blood and induce replication of EBV in B cells (Kekow et al., 1991). Thus an increase in TGF-β1 in saliva of HIV infected individuals on HAART may play a role in higher EBV reactivation and shedding in epithelial cells. EBV viral interleukin-10 (vIL-10) induces marked immunosuppression in synergy with TGF-β1. Moreover, major EBV genes BZLF1 and BRLF1 are involved in EBV lytic replication leading to an increase in EBV shedding (Feederle et al., 2000). Nagata et al (2004) showed saliva from Sjogrens syndrome increased expression of BZLF1 transfected to salivary gland cells. Whether the same is true for HIV saliva on oral mucosal cells is unknown. In the present study we plan to investigate the influence of salivary concentrations of TGF-β1, vIL-10 in saliva of HIV patients on BZLF1 and BRLF1 expression transfected to oral mucosal cell lines (OKF4/6). (Null hypothesis: Salivary TGF-β1 and/or vIL-10 do not induce EBV lytic genes BZLF1 and BRLF1 expression in oral mucosal cells |
| List of Research Outputs |
| Bridges S.M., Botelho M.G., Dyson J.E., McGrath C.P.J., Tsang P.C.S. and Yiu C.K.Y., Blended learning and interactive pedagogies: Supporting problem-based learning in dental education, CITE Research Symposium 2010. 2010. |
| Perera R.A.P.M., Tsang P.C.S., Chan K.H., Lee M.P., Li P., Miller C.S. and Samaranayake L.P., Cytokine Interactome Alterations Promote Oropharyngeal Epstien-barr Virus (EBV) Replication During HIV Infection, Gene Expression Omnibus (GEO) Series GSE21339. 2010. |
| Perera R.A.P.M., Tsang P.C.S., McMillan A.S., Miller C.S., Lee M.P., Li P. and Samaranayake L.P., Oral chemokine interactome unfolds potential anti-HIV mucosal microbicide targets, 23rd IADR Annual Scientific Meeting . 2009. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., A comparison of laboratory extraction techniques for the detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients., Oral Oncology. 2009, 3: 200. |
| Pow E.H.N., Shan J.J.W., Tsang P.C.S., McMillan A.S. and Kwong D.L.W., Detection of Epstein-Barr virus in saliva of nasopharyngeal carcinoma patients: a comparison of two laboratory extraction techniques, Conjoint Scientific Sessions Faculty of Dentistry, The University of Hong Kong - Peking University School of Stomatology. 2009. |
| Tsang P.C.S., Associate editor, In: Tsang Chiu Shun Peter, Journal of Dentistry, Oral Medicine and Dental Education. Scientific Journals International, 2009. |
| Tsang P.C.S. and Tang D.Y.K., Effect of surface treatments of titanium on amphotericin B-treated Candida albicans persister cells, Mycoses. 2009. |
| Tsang P.C.S., Shibu T. and Perera R.A.P.M., Exoenzyme activities of Candida albicans in HIV-infecetd patients on HAART, 23rd IADR Annual Scientific Meeting . 2009. |
| Tsang P.C.S. and Samaranayake L.P., Immune reconstitution inflammatory syndrome after highly active antiretroviral therapy: a review, Oral Diseases. 2010, 16: 248-56. |
| Researcher : Tsang WK |
| Project Title: | Characterization of highly active, thermostable mutants of Trigonopsis variabilis D-Amino acid oxidase for antibiotics production: Kinetic measurements, structural studies and beyond |
| Investigator(s): | Tsang WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2008 |
| Completion Date: | 06/2010 |
| Abstract: |
| 1) To measure the kinetic parameters among wild type TvDAAO, T53P and T53S variants; 2) to establish and compare the crystal structures of wild type TvDAAO and T53P variant and their substrate binding characteristics with cephalosporin C; 3) to elucidate the mechanism for enhanced catalytic activity and thermostability of T53P variant; 4) to further improve the catalytic activity and substrate specificity of TvDAAO by structure-based engineering. |
| Project Title: | Generation of focused libraries by combinatorial biocatalysis with anti-Candidal activity: synthesis and in vitro study |
| Investigator(s): | Tsang WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Research Fund for the Control of Infectious Diseases - Mini Grants |
| Start Date: | 01/2009 |
| Abstract: |
| To identify novel compounds with anti-Candidal activity, a compound library (n= 400) was screened. One compound, #205, was identified (MIC: 20microM) to inhibit growth of C. albicans; to further improve the pharmacological profile of this lead compound; to generate an array of focused libraries by acylation based on compound#205 using immobilized lipase; Modified acyl-derivatives of compound #205 will be evaluated by in vitro assay to delineate structure-activity relationship. |
| Project Title: | Phytate utilization in Candida albicans: possible link to pathogenesis? |
| Investigator(s): | Tsang WK, Samaranayake LP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 06/2010 |
| Abstract: |
| Candida albicans is one of the most prevalent human fungal pathogens. In healthy individuals, C. albicans exists as part of the normal microflora on the skin and on mucosal surfaces of the mouth, digestive tract and urogenital system, without any clinical symptoms. In patients that are immunocompromised – for example, with acquired immunodeficiency syndrome (AIDS), cancer or organ transplantation – this opportunistic pathogen can become invasive and cause an infection (candidiasis). Mild superficial infection such as oral thrush is not fatal; but deep-seated and disseminated candidiasis can be life-threatening. In fact, candidiasis has been ranked fourth among the leading causes of nocosomial infections (Corner and Magee, 1997). The ability of pathogenic microorganisms to colonize and proliferate in the hosts contributes greatly to their pathogenicity. The pathogens should be able to obtain essential nutrients within the hosts. They have developed the capabilities to either synthesize the nutrients de novo or acquire them from the hosts (Reynolds, 2009). Virulence and nutrient acquisition are related. In C. albicans, auxotrophic ade2, hem3 and ura3 mutants are avirulent (Kirsch and Whitney, 1991), whereas other amino acids auxotrophic mutants (e.g. arg4, his1, leu2) exhibit wild type virulence in mouse model of candidiasis (Noble and Johnson, 2005). These results suggested that C. albicans is able to acquire essential nutrients either by de novo synthesis or from the hosts. Phosphate and inositol are essential nutrients and play important role in C. albicans. Phosphate is the building block of nucleic acids, ATP, proteins and lipids. It also involves in phosphorylation and glycolysis. The level of inorganic phosphate in the environment is often limiting and pathogenic microorganisms usually possess efficient systems for their growth and survival under different phosphate-limiting conditions. A recent survey of the phenotypic properties of the four major C. albicans strain clades indicated that phosphate uptake, metabolism and storage may play a role in virulence (MacCallum et al., 2009). Inositol is important for growth and involves in membrane formation, signal transduction and osmoregulation. It is also the precursor of glycosylphatidylinositol (GPI)-anchored glycolipids. The cell surface GPI-anchored glycolipids have been implicated to the pathogenicity of C. albicans through their interactions to human macrophages (Trinel et al., 1999). In C. albicans, inositol is either synthesized de novo from glucose-6-phosphate or imported from the environment via inositol transporter (Reynolds, 2009). The two systems are complementary, rendering C. albicans to exhibit full virulence under different conditions. Nevertheless, double mutants (inositol synthesis and transport) of C. albicans were avirulent and nonviable (Chen et al., 2008). Phytate (myo-inositol hexakisphosphate or myo-inositol 1,2,3,4,5,6-hexakis dihydrogen phosphate) is the major storage form of phosphorus in plants, representing > 60% of total phosphorus content (Lei and Porres, 2003). It also occurs in legumes, cereals and oilseeds used in animal and human diets. Phytate readily forms complexes with zinc, iron, magnesium and copper. Phytase is a phosphohydrolase and hydrolyzes phytate in a stepwise manner to release inorganic phosphate and inositol. Phytate degradation also improves the bioavailability of essential metal ions for absorption and assimilation. Phytase has been correlated to virulence in plant pathogen Xanthomonas oryzae pv. oryzae (Chatterjee et al., 2003) and fish intestinal bacteria of Atlantic cod Gadus morhua (Lazado et al., 2009). Phytase mutant of X. oryzae pv. oryzae was unable to cause infection in rice and expression analysis showed that phytase expression is subject to external phosphate concentration. In the fish intestinal bacteria, it has been suggested that phytase may play a role in incapacitating the functioning of leucocytes. Candida species possess phytase activity and a putative phytase gene (orf19.3754) has been annotated in the genome of C. albicans (http://www.candidagenome.org). The open reading frame contains 462 amino acid residues and shares homology to other yeast phytases (Kaur et al., 2007; Li et al., 2009). Two consensus motifs, RHGXRXP and HD, are evident in the C. albicans phytase. The phytase is located on the cell surface, suggesting its potential involvement in C. albicans-host interactions. The C. albicans phytase gene is regulated by zinc finger transcription factor Rim101p. Rim101p is important for the pH-dependent yeast-hyphal morphological transitions in C. albicans and regulates the expression of Sap (secreted aspartyl proteinase) genes, including SAP5, whose expression has been linked to virulence (Peñalva and Arst, 2002). Thus, Rim101p has become evident to play an intimate role in C. albicans pathogenesis. By now, nothing is known about the contribution of phytase to C. albicans pathogenesis. To cast light on the connection between phytase activity and C. albicans pathogenicity, in the proposed study, the functional role of phytase will be examined. The phytase gene (orf19.3754) will be disrupted to generate heterozygous and homozygous disruptants. The disruptants will be characterized for their ability to exhibit phenotypic determinants, including germ tube and biofilm formation, fungal growth, phytase production and adhesion to buccal epithelial cells. The effect of the transcription factor Rim101p on phytase expression in response to environmental pH will also be assessed. The objectives of the proposed study are: (1) To generate heterozygous and homozygous disruptants of the C. albicans phytase gene (orf19.3754); (2) To examine the phenotypic determinants of the disruptants; and (3) To evaluate the regulatory effect of Rim101p on expression of the C. albicans phytase gene at different pH. |
| List of Research Outputs |
| Kang K., Fong W.P. and Tsang W.K., Mechanistic studies on the metergoline-induced death in Candida krusei, The 2nd International Conference on Drug Discovery and Therapy. 2010. |
| Kang K., Fong W.P. and Tsang W.K., Novel antifungal activity of purpurin against Candida species in vitro, Medical Mycology. 2010, 48: 904-911. |
| So C.W., Tsang W.K., Lo P.C., Seneviratne C.J., Samaranayake L.P. and Fong W.P., Photodynamic inactivation of Candida albicans by BAM-SiPc, Mycoses. 2010, 53: 215-220. |
| Tsang W.K., Wong K.S. and Chu J.K.M., Isolation and characterization of Candida kefyr orotidine-5'-phosphate decarboxylase (URA3) gene, Yeast. John Wiley & Sons, Ltd, 2010, 27: 53-58. |
| Wang J., Tsang W.K., Yap H.K., Chen J.M., Siu Y.L. and Tsang S.Y., Two-step enzyme method for preparing 7-aminocephalosporanic acid, US Patent. 2010. |
| Wong K.S., Fong W.P. and Tsang W.K., A single Phe54Tyr substitution improves the catalytic activity and thermostability of Trigonopsis variabilis D-amino acid oxidase, New Biotechnology. Elsevier B.V., 2010, 27: 78-84. |
| Researcher : Tsoi KH |
| List of Research Outputs |
| Tsoi K.H., Letters : Mercury in the mix, Chemistry World. 2010, 7: 40. |
| Researcher : Tsui VWK |
| List of Research Outputs |
| Tsui V.W.K., Alkhal H.A., Hou H.M., Wong R.W.K. and Rabie A.B.M., The modified two-by-one fixed orthodontic appliance for bodily movement of canine: a case report, Cases Journal 2009. 2009, 2: 211. |
| Researcher : Wang CY |
| List of Research Outputs |
| Jin L.J., Lu Q., Darveau R.P., Samaranayake L.P. and Wang C.Y., P. gingivalis LPS modulates hBD-2 expression through TLR2 and 4, Journal of Dental Research. 2009, 88 (Spec Iss B): 749 (PAPF/APR). |
| Lu Q., Darveau R.P., Samaranayake L.P., Wang C.Y. and Jin L.J., Differential modulation of human b-defensins expression in human gingival epithelia by Porphyromonas gingivalis lipopolysaccharide with tetra- and penta-acylated lipid A structures, Innate Immunity. 2009, 15: 325-335. |
| Researcher : Wang Y |
| List of Research Outputs |
| Choi M.Y., Wang Y. and Watt R.M., Versatile Enzymatic System For The Production Of Guanosine Polyphosphates, Society for General Microbiology Spring Conference, Edinburgh. 2010, ED02/20. |
| Researcher : Watamoto T |
| List of Research Outputs |
| Seneviratne C.J., Wang Y., Jin L.J., Abiko Y., Watamoto T. and Samaranayake L.P., Shotgun proteomics elucidates the regulatory pathway of Candida biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 254 (PAPF/APR). |
| Researcher : Watt RM |
| Project Title: | Polyphosphate metabolic enzymes as potential targets for anti-tuberculosis and general antibiotic drug development |
| Investigator(s): | Watt RM, Huang J, Tanner JA |
| Department: | Chemistry |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 07/2007 |
| Abstract: |
| Biochemical and biophysical characterization of enzyme activities The four M. tuberculosis genes will be cloned, expressed and purified, and their catalytic activities will be characterized in detail, by screening a large selection of potential substrates for turnover. Biochemical assays will be developed, and the catalytic kinetic parameters will be thoroughly investigated. Special attention will be paid to reversibility, reaction rates, as well as substrate and product binding affinities. Where applicable, assays will be miniaturized and adapted for future high throughput inhibitor screening. Binding and Inhibition studies – synthesis of non-hydrolysable substrate analogues As the selected enzymes putatively share a binding affinity for poly-phosphate, we will synthesize non-hydrolyzable imido-polyphosphate (imido poly-P) molecules, with a variety of chain lengths, for binding and inhibition studies (see appendix 1). Substrate-based affinity chromatography - isolate related enzymes from other bacteria The imido poly-P will be chemically linked to a chromatography resin, to create a novel protein affinity matrix. This will be used in ‘pull-down’ experiments, to identify functionally related proteins within cell extracts prepared from a representative set of (non-pathogenic) bacterial species. |
| Project Title: | New DNA recombination systems for microbial genome engineering and proteome analysis |
| Investigator(s): | Watt RM |
| Department: | Chemistry |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 12/2007 |
| Completion Date: | 11/2009 |
| Abstract: |
| Scope of the Problem Bacteria are the most diverse, most abundant, and most adaptable life forms on our planet. Although we have isolated, cultured and characterized many thousands of these microorganisms – environmental metagenomic sequencing projects have indicated that what we have characterized so far constitutes only a small fraction of what actually exists. As efforts to sequence and annotate the genomes of these new bacterial species have greatly accelerated, the development of general and robust methods that may be used to modify and manipulate the genomes of these organisms has not been so rapid. This is a significant problem, as the lack of convenient methods for genome modification greatly hinders efforts to analyze the functions of encoded genes, especially in comprehensive functional genomic investigations. Although methods exist for efficient and specific chromosome modification within several eubacterial species (most notably for the model organism Escherichia coli) there are no convenient methods for ‘DNA engineering’ within the vast majority of bacteria – many of which are key human pathogens, or are microbes with important industrial uses. Such studies are crucial in several respects: e.g. for the identification of genes of essential function in pathogenic bacteria, which may be targets for future antibiotic therapies; or for the characterization of genes of unknown or suggested function, which may be involved in the microbial production of pharmaceuticals, neutriceuticals or biofuels. I will directly address this issue with the research described within this proposal. For this investigation, I will focus on two relatively disparate species: Laribacter hongkongensis and Vibrio cholerae, which are members of the beta- and gammaproteobacteria classes, respectively. Laribacter hongkongensis was recently identified by researchers in the Microbiology Department at HKU as being 'associated with traveler’s diarrhea’. Being a member of the Neisseriaceae family, it is closely related to both the Chromobacterium and Neisseria genera. Notable members of these genera include C. violaceum, which synthesizes the important anti-tumorigenic natural product violacein; and N. meningitidis and N. gonorrhoeae, which cause bacterial meningitis and gonorrhea, respectively. Vibrio cholerae is the etiological agent of cholera, which is responsible for hundreds of thousands of deaths each year (the majority of which are children in the developing world). As well as being important human pathogens, vibrio species are found in many different marine environments, and are one of the most (if not the most?) abundant bacterial genera on Earth. Other notable members include V. parahaemolyticus and V. vulnificus, which also commonly cause gastro-intestinal diseases. Both these organisms present quite different challenges, which are broadly representative of the problems commonly encountered when attempting to perform general molecular biology techniques within other bacterial species. Therefore, they are excellent representative model organisms to work with, for the development of general bacterial DNA engineering procedures. L. hongkongensis was only recently discovered, and thus far, no methods for targeted gene alteration have been reported. It appears to have a reasonably high transformation efficiency, but does not support the replication of any of the commonly used bacterial plasmids. Consequently, only species-specific ‘shuttle vectors’ (constructed from endogenous cryptic plasmids) may be used for episomal gene expression within this organism. Conversely, V. cholerae has been intensively studied for decades. However, largely due to its poor transformation efficiency, versatile techniques for genetic manipulation have yet to be developed. Thus far, targeted gene manipulations in V. cholerae have been achieved by relatively complex and time-consuming procedures, generally involving the introduction of ‘suicide’ vectors by transduction, conjugation or transformation; which all occur with low efficiencies. Key Objectives For this proposal, I will develop molecular biology ‘tools’ and techniques that can be used to efficiently modify the genomes of both Laribacter hongkongensis and Vibrio cholerae. The materials and procedures developed should be applicable for the modification of the genomes of related bacterial species – many of which are major human pathogens. (These further investigations will be pursued in future projects, funded by outside grants to be sought during 2008 and 2009). Specifically, I will investigate the feasibility of using phage- and prophage-derived proteins that promote efficient homologous recombination between double stranded DNA ‘targeting molecules’ and the chromosome. The best studied of these systems is the ‘Red’ set of proteins from bacteriophage lambda, which comprises Bet and Exo. The Exo protein is a 5’ to 3’ single stranded exonuclease, which degrades the ends of the double stranded DNA ‘targeting molecules’ forming long 5’-single stranded regions. Bet binds to these regions of ssDNA and promotes their annealing with homologous regions on the chromosome (or plasmid). The DNA intermediates formed are ‘resolved’, ‘trimmed’ and nicks are ligated by endogenous host proteins, resulting in the formation of the desired genetic alteration. The Red recombination system has been successfully utilized in E. coli to modify both episomal (e.g. plasmid) and chromosomal DNA with efficiencies that may be as high as 20%. Normal efficiencies are in the range 0.01 to 1%, which necessitates the use of selectable markers such as genes conferring resistance to antibiotics. Using bioinformatic methods, I have identified two different sets of Bet and Exo proteins for use in this investigation, and have initiated a preliminary investigation into their activities. One set is located a putative prophage on the L. hongkongensis genome, identified from (unpublished draft) sequencing data given to me by Prof. Patrick Woo (Microbiology, HKU). The second set are housed on the SXT ‘conjugative genetic element’ that infects V. cholerae, which was supplied by Prof. Matthew Waldor (Harvard Medical School). For this investigation I will: • Characterize the activities of the four identified Bet and Exo recombination proteins in vitro using a variety of biophysical techniques • Determine the biological activities of these two pairs of proteins in vivo (using a genetic reporter system within E. coli) • Introduce these pairs of proteins into L. hongkongensis and V. cholerae either on plasmids (i.e. episomally) or insert them onto the chromosome • Develop protocols for the specific modification of the V. cholerae and L. hongkongensis genomes with DNA ‘targeting molecules’, through homologous recombination-based processes catalyzed by these proteins • Use the DNA engineering techniques and tools generated to formulate methods enabling the detailed analysis of the proteomes of these two bacterial species |
| Project Title: | A Molecular toolbox for genetic, proteomic and metabolic engineering in the ethanol-producing bacterium Zymomonas mobilis |
| Investigator(s): | Watt RM, Huang J |
| Department: | Chemistry |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2009 |
| Abstract: |
| 1) Construct stable and versatile new Z. mobilis – E. coli shuttle vectors for the inducible expression of foreign genes in Z. mobilis; 2) Characterize the activities of two new pairs of Bet/Exo family DNA recombination proteins, using well-established reporter systems in E. coli; 3) Determine the optimal parameters for engineering the Z. mobilis chromosome using shuttle vector-based Bet/Exo DNA recombination proteins; 4) Develop a tandem-affinity purification (TAP) protein labeling technique, which can be used to identify proteins interactions within Z. moblis; 5) Determine the feasibility of engineering Z. mobilis for the production of monterpene and sesquiterpene natural products |
| Project Title: | Oral Treponemes: Proteomic and genetic analysis |
| Investigator(s): | Watt RM, Leung WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 05/2009 |
| Abstract: |
| Periodontal diseases are chronic polymicrobial infections of the tissues and bone surrounding and supporting the tooth. After tooth decay, these are the second most common diseases in the global population, with a prevalence of ca. 30-50% in developed countries (Loesche 2001). Diseases of the human tooth supporting structures range from the relatively mild gingivitis (gum inflammation and bleeding), to highly severe forms including acute, chronic or necrotizing periodontitis. Several hundreds of different bacterial species commonly inhabit the oral cavity. Many of these cooperate to form a tough and structurally-complex biofilm on the tooth surface, commonly referred to as plaque. The plaque biofilm is composed of an inter-dependent, spatially-organized community of bacteria within an extra-cellular polymeric matrix. Plaque may further mineralize to form calculus (tartar), which is extremely resistant to physical removal (e.g. by brushing or flossing). The formation and calcification of plaque is exacerbated by poor oral hygiene and healthcare, enabling it to encroach below the gum line, into the shallow gap between the tooth and the gum (the sulcus). If untreated, this gradually leads to the formation of a deep pocket between the tooth and gingiva (gum), which is low in oxygen. This greatly alters the dynamic composition of the bacterial flora present within the sub-gingival plaque; with certain anaerobic species being significantly enriched (see below). Bacterial (endo-)toxins and other secreted or surface virulence factors cause severe localized inflammation of the gingiva (gums). A number of bacterial species are known to adhere to and invade the epithelial cells within the periodontal pocket and proteolytically degrade structural and connective tissues such as collagen and fibronectin. These activities, in combination with the host inflammatory response cause localized necrosis (cell death and tissue damage). Bacterial virulence factors also inhibit the activities of gingival fibroblast (matrix-forming) and cementoblast (cementum-forming) cells. This inhibits bone and cementum regeneration, further weakening the attachment of the tooth. Ultimately, there is severe ‘pocket’ formation, gum recession and eventually tooth loss. There are a small number of anaerobic bacterial species that are strongly associated with periodontal diseases, including: Porphyromonas gingivalis; Fusobacterium nucleatum; Tannerella forsythia; Aggregatibacter actinomycetemcomitans and Treponema denticola (Haffajee 2005, Socranski 2005). Being highly motile, with an unusual spiral-shaped cell morphology, the Gram negative spirochete T. denticola is the most poorly studied of these periodontal pathogens; and is arguably the most biologically interesting. There are ca. 60 treponemal species (or phylotypes) known to inhabit the human mouth, many of which are suspected of playing important roles in periodontal diseases. However, due to the paucity of detailed molecular analyses, only T. denticola (and to a less well-proven extent, T. socranskii) have thus far been demonstrated to be strongly associated with these diseases (Sela 2001, Holt 2005). Due to their fastidious nature, only four oral treponemes can be reliably cultured ex vivo (T. denticola, T. pectinovorum, T. socranskii and T. vincentii) and have been studied in any detail (Chan 2000). As a result, very little is known about their biochemistry, their cellular and molecular biology, or their role in periodontal diseases. Furthermore, very little is known about the global distribution of oral treponeme species, and how this may be related to the frequency or severity of periodontal diseases. T. denticola (ATCC 35405) is the only oral treponeme species whose genome has been sequenced (Seshadri 2004). It has a compact circular genome (2,843,201 nt; 91% coding; 37% G/C) with 2767 protein encoding genes. Its closest homologue is Treponema pallidum (the causative agent of venereal syphilis); although the genome of this obligate human parasite is significantly reduced in size (1.1 Mb), having diverged considerably from a common ancestor. Even though several surface proteins have been identified as potential virulence factors (e.g. major surface protein, Msp) (Ellen 2005, Sela 2001, Holt 2005), thus far, there have been no detailed or systematic analyses performed on the proteome of T. denticola. Consequently, the detailed composition of its surface-exposed or secreted proteins is unknown. This is highly significant, as some of these (as yet unidentified) periplasmic, membrane-integrated or exported proteins are likely to be directly involved in the pathogenesis of this group of bacteria. These proteins may promote co-aggregation or association with other (pathogenic) bacteria, or may directly mediate interactions with fibroblasts, osteoblasts, cementoblasts or the host epithelia cells, to potentiate the periodontal infection. In this proposal, we have two major aims: 1) to characterize the proteins expressed by T. denticola, and 2) identify which treponeme species (phylotypes) are present in heavily- infected periodontal pockets of (Hong Kong Chinese) patients with severe periodontitis. The results of our studies will generate a comprehensive body of data that will significantly expand our current understanding of the pathogenesis of oral treponemes. Our specific objectives are as follows: 1) Characterization of the proteome of Treponema denticola We will characterize a) the ‘total’ proteome of T. denticola grown in liquid and semi-solid culture, i.e. identify the proteins that are expressed to detectable levels within the cell. We will further identify the proteins that are associated with the membrane, as well as those that are exported into the periplasm or secreted from the cell. We will also generate the first set of annotated proteome ‘reference maps’ for this organism. 2) Genetic analyses of oral treponemes in patients with periodontal diseases We will identify the treponemal species (genetic phylotypes) present within sub-gingival plaque taken from patients with severe periodontitis, using 16S ribosomal RNA gene sequence analysis. Samples from both diseased and ‘healthy’ periodontal pockets will be analyzed and compared. We will establish the phylogenetic diversity of species present within these sites, and will compare and contrast our findings with data obtained from related molecular analyses of periodontal pathogens (Aas 2005, Choi 1994, Dewhirst 2000, Kumar 2005, Paster 2001). |
| Project Title: | Genes get 'recT' : new and improved methods for bacterial DNA engineering |
| Investigator(s): | Watt RM, Huang J, Tanner JA |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2010 |
| Abstract: |
| 1) Optimize conditions for LHK-Bet-mediated ssDNA recombination in E. coli; 2) Investigate relative dsDNA recombination activities of three newly-identified sets of recombinase/exonuclease proteins in E. coli; 3) Develop recombineering technologies within L. hongkongensis and N. lactamica; 4) Establish which conserved structural elements and regions within the Bet/RecT family are involved in: a) DNA recombination activity; b) DNA binding and strand exchange activities; c) determining recombinase/exonuclease binding specificity; d) modulating oligomerization and nucleofilament formation. |
| Project Title: | Society for General Microbiology Spring 2010 Meeting Versatile enzymatic system for the production of guanosine polyphosphates |
| Investigator(s): | Watt RM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 03/2010 |
| Completion Date: | 04/2010 |
| Abstract: |
| N/A |
| Project Title: | A molecular typing system for Treponema denticola: an enigmatic oral pathogen |
| Investigator(s): | Watt RM, Leung WK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2010 |
| Abstract: |
| Background The term ‘periodontal disease’ refers to a spectrum of chronic infectious inflammatory diseases of the tissues and bone that surround and support the teeth (Loesche, 2001). These range from the relatively mild gingivitis (gum inflammation and bleeding), to highly severe forms including acute, chronic or necrotizing periodontitis. Periodontal diseases may be considered to be mixed-species, bacterial biofilm diseases (Schaudinn, 2009). They share a common etiology; beginning with the gradual encroachment of tooth-associated biofilms below the gumline (subgingival plaque), leading to an overpopulation of anaerobic, proteolytic bacteria. These ‘periodontal disease-associated pathogens’ progressively degrade the gingival epithelium, creating a deep and persistent ‘pocket’ of infection around the tooth. Bacterial products such as lipolysaccharides (LPS), toxins and proteases directly cause host cell and tissue damage. They also elicit the release of cytokines and other inflammatory mediators that promote the resorption of alveolar bone and the destruction of the tooth-attachment and supporting tissues, ultimately leading to tooth loss. The etiology of periodontal disease is extremely complex as it involves a combination of host, environmental and microbiological factors. Elevated populations of the oral spirochete bacterium Treponema denticola are strongly associated with the most severe forms of periodontal disease (Sela, 2001). More than 60 species (or phylotypes) of spirochetes have been identified in the oral cavity; all of which belong to the genus Treponema (Dewhirst, 2000). Due to their fastidious nature, less than a quarter of these are cultivatable. This has severely hampered efforts to study and compare their genetic composition, physiology and biology; and to identify common or unique pathogenic attributes. T. denticola is the most highly-studied oral spirochete, and is used as a model organism for several uncultivatable (non-oral) pathogenic species such as Treponema pallidum, the causative agent of venereal syphilis. T. denticola was the first and only oral spirochete to have its genome sequenced (Seshadri, 2004). With such limited genetic information available, no systematic comparative genetic investigations or genetic typing systems have been developed for any treponeme species. After complete genome sequencing, which is expensive and impractical for routine use, multi locus sequence typing (MLST) is the best way to unambiguously identify or characterize a particular microbial isolate (Urwin, 2003). This molecular typing technique is invaluable for the rapid identification of pathogenic strains for epidemiological purposes. Notable pathogens for which MLST systems have developed include: Candida albicans, Neisseria meningitidis and Staphylococcus aureus. It is also a powerful tool for studying evolutionary and population biology. The basic principle of MLST involves using consensus primer sets to PCR-amplify and sequence 300-600bp sections from 7-10 ‘reference’ or ‘housekeeping’ genes within the isolate of interest. Genetic variations or ‘alleles’ within each of these reference genes are then compared against a large reference dataset (e.g. at the online repository at www.mlst.net). To construct an MLST reference database, the sequences for these 7-10 reference genes must first be determined within a large set of isolates of diverse origin. A World-class Microbiological Resource The world’s pre-eminent expert on the biology and culture of oral treponeme bacteria: Dr. Chris Wyss of the University of Zurich retired this summer. He has generously gifted the PI and CoI his entire collection of treponeme strains, which he has isolated and accrued over the past 25 years. In essence, he has given us the world’s most comprehensive collection of oral treponeme species; comprising >120 diverse isolates and reference strains belonging to over 15 species, obtained from animal as well as human sources. Some of the more poorly-characterized ones have unusual cell morphologies, atypical phenotypic or biochemical profiles, and may even constitute novel species. Over the past few months we have begun culturing representative stocks, to check the viability of the collection. We have confirmed that the stocks are viable, and the collection is accurately labeled. Scope of the Problem The diversity, phylogeny and ancestry of T. denticola have never been directly investigated. This is an important issue, as researchers who are investigating the biology and pathology of T. denticola have used a variety of different ATCC reference strains or well-studied clinical isolates. Although these strains have distinct cell morphologies, phenotypic properties and serotypes, nobody has ever systematically investigated how similar or different these strains are at the genetic level. T. denticola plays a key role in destructive periodontal diseases, but we have no idea which strains are the most common or virulent within the oral cavity. We presently do not have the genetic knowledge or diagnostic tools to answer this question. Here, we will directly address these key issues. Our working hypothesis is that there is a subset of T. denticola strains that have increased virulence within infected periodontal pockets, which cause a high level of tissue damage and inflammation. The MLST system and genetic information developed through this project will enable us, and other teams of oral microbiologists, to test this and numerous other working hypotheses. Objectives in point form 1) Determine the sequences of eight ‘reference’ genes within 16 diverse strains of T. denticola. 2) Develop a multi locus sequence typing (MLST) system for T. denticola. The sequence dataset from objective (1) will be used to guide the design of the consensus primers used for this MLST system. 36 diverse strains of T. denticola isolated from humans, cats and dogs will be used to form the reference set. 3) Analyze the phylogeny of each gene within all the strains studied using a variety of computational methods. This will enable us to gauge overall genetic diversity within this species, and establish the ancestry and ‘relatedness’ of the most highly used laboratory strains. |
| List of Research Outputs |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development, BMC Microbiology. 2010, 10: 125 (pages 1-9). |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation, Journal of Medical Microbiology. 2009, 58: 1623-1631. |
| Choi M.Y., Wang Y. and Watt R.M., Versatile Enzymatic System For The Production Of Guanosine Polyphosphates, Society for General Microbiology Spring Conference, Edinburgh. 2010, ED02/20. |
| Huang J., Sun H., Tanner J.A. and Watt R.M., Determine the functions of the putative metal-binding domain of SARS-CoV helicase, Hong Kong Medical Journal. 2009, 15(5) Supplement: 6:15-16. |
| Jin Y., Watt R.M., Danchin A. and Huang J., Use of a riboswitch-controlled conditional hypomorphic mutation to uncover a role for the essential csrA gene in bacterial autoaggregation, Journal of Biological Chemistry. 2009, 284: 28738-28745. |
| Lui E.L.H., Shum K.T., Sam L., Wang Y., Watt R.M. and Tanner J.A., Aptamers Against Polyphosphate Kinase 2 (PPK2) From Mycobacterium Tuberculosis As A Potential Approach Of Novel Anti-Tuberculosis Therapy , CUHK Croucher Advanced Study Institute “Structure-Based Screening and Design of Ligands for Protein Targets” . 2009. |
| Shum K.T., Lui E.L.H., Wang Y., Watt R.M. and Tanner J.A., Aptamer-mediated inhibition of Mycobacterium tuberculosis polyphosphate kinase 2. , 2010 Hong Kong Inter-University Biochemistry Postgraduate Symposium . 2010. |
| Tanner J.A. and Watt R.M., Common Core Teaching Development Grant, 2010. |
| You M., Mo S., Leung W.K. and Watt R.M., Diversity of oral treponemes in patients with chronic periodontitis, Journal of Dental Research. 2009, 88 (Spec Iss B): 377 (PAPF/APR). |
| Zhou J., Huang J., Poon K.M., Chen D., Chan C.S., Ng F., Guan X.Y., Watt R.M., Lu L., Yuen K.Y. and Zheng B., Functional dissection of an IFN-alfa/belta receptor 1 promoter variant that confers higher risk to chronic hepatitis B virus infection. , J Hepat.. 2009, 51(2):: 322-332. |
| Researcher : Wong AHH |
| List of Research Outputs |
| Wong A.H.H., You D.S.H., Chan L.S.Y., Chan W.Y., Cheung A.C.H., Lam K.W., Liu W.Y., Wong W.K., Yeung P.M., Yim P.K. and Yun L., A clinical trial on the effect of modified manual toothbrush and electric toothbrush and electric toothbrush on oral hygiene and gingival health of Hong Kong stroke patients, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 165: 1-75. |
| Researcher : Wong CM |
| Project Title: | Trends in oral health from childhood to early adulthood: a life-course study |
| Investigator(s): | Wong MCM, Lo ECM, McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2008 |
| Completion Date: | 12/2009 |
| Abstract: |
| To investigate the trends in oral health in the cohort from childhood, to adolescence and early adulthood; to investigate how the oral health of young adults is affected by their oral health, oral health behavior during their childhood and/or adolescence and the family socio-demographic characteristics. |
| Project Title: | Topical fluoride in causing dental fluorosis in children |
| Investigator(s): | Wong MCM, Lo ECM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2009 |
| Abstract: |
| The primary objective of this systematic review is to evaluate the available evidence of different topical fluoride treatment modalities (including gels, toothpastes, varnishes and mouthrinses) used at different concentrations, and at varying duration of use/exposure in causing dental fluorosis in children with different exposure to systemic fluoride (such as water or salt fluoridation). This review attempts to answer the question on whether use of topical fluorides in young children will increase their risk of developing dental fluorosis. |
| Project Title: | Trends in oral health of preschool children in Hong Kong |
| Investigator(s): | Wong MCM, Lo ECM, Lu HX |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2010 |
| Abstract: |
| The objectives of this study are: 1. To investigate the trends in oral health in the preschool children cohort from 3-4 years old to 5-6 years old. 2. To investigate how the oral health of 5-6 years old preschool children is affected by their oral health, oral health behavior when they were younger as well as parents’ oral health knowledge, attitude, and the family socio-demographic characteristics. |
| Project Title: | 4th International Meeting on Methodological Issues in Oral Health Research Systematic Review on topical fluoride as a cause of dental fluorosis in children |
| Investigator(s): | Wong MCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 04/2010 |
| Completion Date: | 04/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Researcher : Wong HM |
| Project Title: | Parental perceptions of oral health-related quality of life for young children |
| Investigator(s): | Wong HM, McGrath CPJ, Lo ECM, King NM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2008 |
| Abstract: |
| 1. To assess the impact of oral health problems and related treatment experiences on the quality of life in a population of preschool children and their families. 2. To investigate the effects of early childhood caries (ECC) and related habits on children's oral health-related quality of life. 3. To investigate the effects of sociodemographic characteristics of the parents on reporting their children's oral health-related quality of life. |
| List of Research Outputs |
| Bridges S., McGrath C.P.J., Wong H.M., YIU C.K. and Au T.K.F., Exploring the dimensions of oral health literacy, The 9th World Congress on Preventive Dentistry. 2009. |
| Bridges S.M., McGrath C.P.J., Wong H.M. and Au T.K.F., Exploring The Dimensions Of Oral Health Literacy. , In: Bridges, S. M., McGrath, C. P. J., Wong, H. M., & Au, T. K. , The 9th World Congress On Preventive Dentistry, Phuket, Thailand.. 2009. |
| King N.M., Tsai J.S.J. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part I: Anomalies in the permanent dentition, The Open Anthropology Journal. 2010, 3: 54-64. |
| King N.M., Tsai J.S.J. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part II: Traits in the permanent dentition , The Open Anthropology Journal. 2010, 3: 71-84. |
| King N.M., Tongkoom S. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part III: Anomalies in the primary dentition, The Open Anthropology Journal. 2010, 3: 25-36. |
| King N.M., Tongkoom S. and Wong H.M., Morphological and numerical characteristics of the southern Chinese dentitions. Part IV: Traits in the primary dentition, The Open Anthropology Journal. 2010, 3: 37-53. |
| Sanares A.M.E., King N.M., Itthagarun A. and Wong H.M., Chewing gum as a medium for the delivery of anticariogenic therapeutic agents: a review , Hong Kong Dental Journal. 2009, 6: 13-22. |
| Wong H.M., Oral health-related quality of life in Hong Kong preschool children, Advanced Diploma in Dental Surgery (Paediatric Dentistry) Thesis. 2009. |
| Wong H.M., McGrath C.P.J., King N.M. and Lo E.C.M., Oral health-related quality of life in Hong Kong preschool children, Conjoint Scientific Sessions, The University of Hong Kong - Peking University. 2009. |
| Yang Y., Wong H.M. and Chai L., Poster Presentation: Attitudes of BDSI and BDSII Students to Self-assessment of Performance in PBL Tutorials, The 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 Nov. 2009. |
| Yang Y.Q., Wong H.M. and Chai L., Attitudes of BDSI and BDSII students to self-assessment of performance in PBL tutorials, 6th International Conference on PBL in Dentistry. 2009. |
| Researcher : Wong MCM |
| Project Title: | Trends in oral health from childhood to early adulthood: a life-course study |
| Investigator(s): | Wong MCM, Lo ECM, McGrath CPJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2008 |
| Completion Date: | 12/2009 |
| Abstract: |
| To investigate the trends in oral health in the cohort from childhood, to adolescence and early adulthood; to investigate how the oral health of young adults is affected by their oral health, oral health behavior during their childhood and/or adolescence and the family socio-demographic characteristics. |
| Project Title: | Topical fluoride in causing dental fluorosis in children |
| Investigator(s): | Wong MCM, Lo ECM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2009 |
| Abstract: |
| The primary objective of this systematic review is to evaluate the available evidence of different topical fluoride treatment modalities (including gels, toothpastes, varnishes and mouthrinses) used at different concentrations, and at varying duration of use/exposure in causing dental fluorosis in children with different exposure to systemic fluoride (such as water or salt fluoridation). This review attempts to answer the question on whether use of topical fluorides in young children will increase their risk of developing dental fluorosis. |
| Project Title: | Trends in oral health of preschool children in Hong Kong |
| Investigator(s): | Wong MCM, Lo ECM, Lu HX |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2010 |
| Abstract: |
| The objectives of this study are: 1. To investigate the trends in oral health in the preschool children cohort from 3-4 years old to 5-6 years old. 2. To investigate how the oral health of 5-6 years old preschool children is affected by their oral health, oral health behavior when they were younger as well as parents’ oral health knowledge, attitude, and the family socio-demographic characteristics. |
| Project Title: | 4th International Meeting on Methodological Issues in Oral Health Research Systematic Review on topical fluoride as a cause of dental fluorosis in children |
| Investigator(s): | Wong MCM |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 04/2010 |
| Completion Date: | 04/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Cheung L.K., Leung Y.Y., Chow L.K., Wong M.C.M., Chan E.K.K. and Fok Y.H., Incidence of neurosensory deficits and recovery after lower third molar surgery: a prospective clinical study of 4338 cases, International Journal of Oral and Maxillofacial Surgery. 2010, 39: 320-326. |
| Li K.Y., Wong M.C.M., Lam K.F. and Schwarz E., Marginal approach in analyzing sequential cross-sectional oral health survey data, Journal of Dental Research. 2009, 88 (Spec Iss B): 311 (PAPF/APR). |
| Lu H.X., Wong M.C.M., Lo E.C.M. and McGrath C.P.J., Factors associated with oral health from childhood to early adulthood, Journal of Dental Research. 2009, 88 (Spec Iss B): 313 (PAPF/APR). |
| McMillan A.S., Wong M.C.M., Zheng J., Luo Y. and Lam C.L.K., Widespread pain symptoms and psychological distress in southern Chinese with orofacial pain, Journal of Oral Rehabilitation. 2010, 37: 2-10. |
| Wong M.C.M., Associate Editor, Community Dentistry and Oral Epidemiology. Wiley-Blackwell, 2010. |
| Wong M.C.M., Best Original Article Award 2008, Hong Kong Dental Journal. 2009. |
| Wong M.C.M., Editorial Board Member, Clinical Oral Implants Research. Wiley-Blackwell, 2010. |
| Wong M.C.M., Lu H.X., Lo E.C.M. and McGrath C.P.J., Oral health status of young adults in Hong Kong, Journal of Dental Research. 2009, 88 (Spec Iss B): 308 (PAPF/APR). |
| Wong M.C.M., Lu H.X., Chan K.F., Chan T.K., Chung W.Y., Leung M.Y., Mak Y.K., Pu Y.C., Wong S.S.S., Wong W.Y. and Yeung H.W., Smoking cessation in dental practices - The perspectives of the dentists in Hong Kong, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 164: 1-43. |
| Wong M.C.M., Glenny A.M., Tsang B.W.K., Lo E.C.M., Worthington H.V. and Marinho V.C.C., Systematic review on topical fluoride as a cause of dental fluorosis in children, 4th International Meeting of Methodological Issues in Oral Health Research. 2010. |
| Wong M.C.M., Glenny A.-.M., Tsang B.W.K., Lo E.C.M., Worthington H.V. and Marinho V.C.C., Topical fluoride as a cause of dental fluorosis in children, Cochrane Database of Systematic Reviews. 2010, Issue 1: 1-32. |
| Zheng J., McMillan A.S., Wong M.C.M., Luo Y. and Lam C.L.K., Investigation of factors associated with treatment-seeking in southern Chinese with orofacial pain, Journal of Orofacial Pain. 2010, 24: 181-188. |
| Researcher : Wong RWK |
| Project Title: | The use of Monascus purpureus in bone induction |
| Investigator(s): | Wong RWK, Rabie ABM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2008 |
| Completion Date: | 06/2010 |
| Abstract: |
| Objective: Bone induction is used to replace bone lost due to trauma, tumours and other pathological diseases. Over the years autogenous cancellous bone grafting has been considered the gold standard in this form of treatment. The major limitations of using autogenous grafting are the inadequacy of supply and surgical morbidity; including donor site pain, paraesthesia, and infection, which can approach 8% to 10% (Younger and Chapman 1989). Moreover, graft resorption poses a severe problem. In an experimental study, endochondral bone grafts showed 65% volume loss (Zins and Whitaker 1983). Allografts, an alternative to autogenous grafting, seem to be biologically inferior and are associated with infection and inflammation (Strong et al. 1996). Therefore, the search of an ideal bone grafting material that induces bone is an important issue in bone research. Bone morphogenetic proteins (BMPs) are important regulators in bone formation (Sakou 1998). Mundy et al. (1999) demonstrated that statin, a hydroxymethylglutaryl coenzyme A (HMG–CoA) reductase inhibitor (HMGRI), specifically increased expression of the BMP-2 gene. We showed that statin increased new bone formation by more than 300% in vivo (Wong and Rabie 2003). Further studies have shown that this BMP-2 activation was a result of enzyme inhibition (Sugiyama et al. 2000). Therefore, it is possible that any drug that inhibits the HMG-CoA reductase may have a similar effect to statin in the activation of BMP-2. Monascus purpureus (Hongqu, 紅曲) is red yeast that produces a fermented product of rice (Red yeast rice), has been used in Chinese cuisine (like Peking Duck and red sausages) and as a traditional Chinese medicine for centuries (Li et al., 1998). Red yeast rice forms naturally occurring HMGRIs known as monacolins. One of them, Monacolin K, is equivalent to the statin called known as mevinolin or lovastatin. However, red yeast rice also contains a family of eight other monacolins which are also HMGRIs. Other active ingredients in red yeast rice include sterols (β-sitosterol, campesterol, stigmasterol, sapogenin), isoflavones, and monounsaturated fatty acids (Heber et al., 1999).. It is therefore possible that Monascus purpureus may also activate BMP-2 and increase bone formation. To examine further the role of Monascus purpureus in clinical use for the repair of bone defects, it is important to examine its osteoinductivity in the presence of a carrier and to examine its healing route in an in vivo study. Aims: • To test the hypothesis that Monascus purpureus is effective for bone induction. • To examine the ability of Monascus purpureus with a carrier in bone induction by quantitatively assessing the amount of new bone formation with and without Monascus purpureus. • To identify the action mode and effects of Monascus purpureus on the gene expression profile in a Monascus purpureus-treated osteoblastic cell line cultured in vitro using molecular biology techniques. |
| Project Title: | Research Output Prize |
| Investigator(s): | Wong RWK, Rabie ABM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Research Output Prize (in Faculty) |
| Start Date: | 10/2008 |
| Abstract: |
| To identify and recognize the best research outputs in different faculties. |
| Project Title: | The use of policosanols in bone induction |
| Investigator(s): | Wong RWK, Rabie ABM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2009 |
| Abstract: |
| Objective: Bone induction is used to replace bone lost due to trauma, tumours and other pathological diseases. Over the years autogenous cancellous bone grafting has been considered the gold standard in this form of treatment. The major limitations of using autogenous grafting are the inadequacy of supply and surgical morbidity; including donor site pain, paraesthesia, and infection, which can approach 8% to 10% (Younger and Chapman 1989). Moreover, graft resorption poses a severe problem. In an experimental study, endochondral bone grafts showed 65% volume loss (Zins and Whitaker 1983). Allografts, an alternative to autogenous grafting, seem to be biologically inferior and are associated with infection and inflammation (Strong et al. 1996). Therefore, the search of an ideal bone grafting material that induces bone is an important issue in bone research. Bone morphogenetic proteins (BMPs) are important regulators in bone formation (Sakou 1998). Mundy et al. (1999) demonstrated that statin, a hydroxymethylglutaryl coenzyme A (HMG–CoA) reductase inhibitor (HMGRI), specifically increased expression of the BMP-2 gene. We showed that statin increased new bone formation by more than 300% in vivo (Wong and Rabie 2003). Further studies have shown that this BMP-2 activation was a result of enzyme inhibition (Sugiyama et al. 2000). Therefore, it is possible that any drug that inhibits the HMG-CoA reductase may have a similar effect to statin in the activation of BMP-2. Policosanols are a mixture of aliphatic alcohols derived from purified sugar cane. Policosanols are thought to improve lipid profiles by reducing hepatic cholesterol biosynthesis while enhancing LDL clearance. When compared with statins, policosanols exhibit comparable cholesterol lowering effects at much smaller doses. Menendez et al suggests that policosanols could possibly suppress the action of HMG-CoA reductase, a key enzyme in the first step of cholesterol biosynthesis that functions to convert acetyl CoA into mevalonate. It is therefore possible that policosanols may also activate BMP-2 and increase bone formation. To examine further the role of policosanols in clinical use for the repair of bone defects, it is important to examine its osteoinductivity in the presence of a carrier and to examine its healing route in an in vivo study. Aims: • To test the hypothesis that policosanols are effective for bone induction. • To examine the ability of policosanols with a carrier in bone induction by quantitatively assessing the amount of new bone formation with and without policosanols. • To identify the action mode and effects of policosanols on the gene expression profile in a policosanols -treated osteoblastic cell line cultured in vitro using molecular biology techniques. |
| Project Title: | Effect of puerarin on growth responses of mouse cranial bases (synchondroses). |
| Investigator(s): | Wong RWK, Rabie ABM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2010 |
| Abstract: |
| To investigate the effect of puerarin on growth of mouse cranial bases (synchondroses) in organ culture. |
| Project Title: | 7th International Orthodontic Congress The response of the Spheno-Occipital Synchondrosis to Mechanical Stress |
| Investigator(s): | Wong RWK |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 02/2010 |
| Completion Date: | 02/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Al Khal H.M., Rabie A.B.M. and Wong R.W.K., Orthodontic tooth movement of total buccally blocked-out canine: a case report, Cases Journal 2009. 2009, 2: 7245. |
| Albaker B., Wong R.W.K. and Rabie A.B.M., Dentoskeletal effects of Herbst Appliance treatment among different skeletal maturity groups, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p27 (HP001). |
| Almoqubel A., Wong R.W.K., Zuo P. and Rabie A.B.M., Poster Presentations: Stem Cell Differentiation into Angiogenesis Lineage, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Anderson I.M., Rabie A.B.M. and Wong R.W.K., Poster Presentations: Early Treatment of Pseudo Class III Malocclusion – A 10-Year Follow-Up Study, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Bootvong K., Liu Z., McGrath C.P.J., Hagg E.U.O., Wong R.W.K., Bendeus S.A.M. and Yeung S., Virtual model analysis as an alternative approach to plaster model analysis: reliability and validity, The European Journal of Orthodontics. 2010, 32: 589-595. |
| Cendekiawan T., Wong R.W.K. and Rabie A.B.M., Relationships Between Cranial Base Synchondroses and Craniofacial Development: A Review, The Open Anatomy Journal 2010. 2010, 2: 67-75. |
| Chaiyongsirisern A., Rabie A.B.M. and Wong R.W.K., Treatment effects and long-term stability of adult Class II patients treated with the Stepwise Advancement Herbst appliance, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p27 (HP004). |
| Chau C.M., Wong R.W.K. and Hagg E.U.O., 3-Dimensional Cone-Beam Computerized Tomographic Cephalometric Database on Jaw Dimensions in Chinese, The Open Anthropology Journal 2010. 2010, 3: 2-7. |
| Chau C.M., Hagg E.U.O. and Wong R.W.K., Oral Presentations: Technical Concerns of 3D Cephalometric Measurement Using CBCT Images, The XIIth European Congress of Dento-Maxillo Facial Radiology, Istanbul Turkiye, 2 – 5 June, 2010. |
| Chau C.M. and Wong R.W.K., Use of Cone Beam CT in Orthodontics, Oral Health and Science Seminars. 31 March. 2010. |
| Chen K., Rabie A.B.M. and Wong R.W.K., Effects of LEPR fa/fa Mutation on Mandible Dimension and Their Impact and Diagnosis and Treatment of Mandibular Retrognathism, 1st Hong Kong International Dental Expo And Symposium (HKIDEAS) – Hong Kong Convention and Exhibition Centre, 18-20 June, 2010. |
| Chen K., Wong R.W.K. and Rabie A.B.M., Genetic predisposition to skeletal class II malocclusion in a Southern Chinese population, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p142 (SP441). |
| Chen K., Wong R.W.K. and Rabie A.B.M., Poster Presentation: LEPR is a Predisposing Factor of Mandibular Retrognathism; A SNP Association and Animal Study, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. |
| Chen K., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Polymorphisms of LEPR Gene in Mandibular Retrognathism, The 7th International Orthodontic Congress (IOC) World Federation of Orthodontists, Sydney – Australia, 6-9 February, 2010. |
| Chen K., Wong R.W.K. and Rabie A.B.M., Title: LEPR is a Predisposing Factor of Mandibular Retrognathism; A SNP Association and Animal Study , GSK Outstanding Oral Presentation (Junior). Awarded by the Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. 2009. |
| Chen Q.G., Zeng Y.S., Qu Z.Q., Tang J.Y., Qin Y.J., Chung P., Wong R.W.K. and Hagg E.U.O., The effects of Rhodiola rosea extract on 5-HT level, cell proliferation and quantity of neurons at cerebral hippocampus of depressive rats, Phytomedicine 2009. 2009, 16: 830-838. |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Antimicrobial activity of Prunes extract on Streptococcus mutans, Second Prize of E-poster Competition for Postgraduate Students. Awarded by IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. 2010. |
| Chen Y., Wong R.W.K., Hagg E.U.O., McGrath M., Seneviratne C.J., Samaranayake L.P. and Kao R., Poster Presentation: Antimicrobial activity of Prunes extract on Streptococcus mutans, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Chin A., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Medicinal Herb Salvia Miltiorrhiza on Osteoblastic Cell Line of MC3T3-E1, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Chin A., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Salvia Miltiorrhiza on Osteoblastic Cell Line of MC3T3-E1, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Geoghegan F., Wong R.W.K. and Rabie A.B.M., Inhibitory Effect of Quercetin on Periodontal Pathogens in vitro, Phytotherapy Research (online in Wiley InterScience). 2009. |
| Geoghegan F., Tsui V., Wong R.W.K. and Rabie A.B.M., The effects of orthodontic intrusion utilising two novel osteogenic compounds on periodontal bone levels, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June,. 2009, p83 (SP176). |
| Gu M., Rabie A.B.M. and Wong R.W.K., 邊缘骨性III類錯合手述與非手述效果的對比分析, Journal of Oral Science Research. 2009, 25 No.5: 611-614. |
| Gu M., Rabie A.B.M. and Wong R.W.K., 骨性III類錯合手述與非手述邊緣病例的顱面特征分析, 廣東牙病防治, 2009年12月. 2010, Vol 17, No.12: 571-573. |
| Hagg E.U.O., Khanoengnit B., Liu Z., McGrath C.P.J. and Wong R.W.K., Virtual models as an alternative approach to plaster model assessment of tooth dimension, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p106 (SP284). |
| Hamza E., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Effect of Autotransplantation on Bone Regeneration in Orthodontic Patients, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Hibino K., Wong R.W.K., Hagg E.U.O. and Samaranayake L.P., The effects of orthodontic appliances on Candida in the human mouth, The International Journal of Paediatric Dentistry . 2009, 19: 301-308. |
| Hibino K., Samaranayake L.P., Hagg E.U.O., Wong R.W.K. and Lee W., The role of salivary factors in persistent oral carriage of Candida in orthodontic patients, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p151 (SP475). |
| Ikegami T., Wong R.W.K., Lee W., Hibino K. and Hagg E.U.O., The Hybrid Orthodontic Treatment System (HOTS), World Journal of Orthodontics. 2010, 11: 168-179. |
| Lee W. and Wong R.W.K., Hong Kong Dental Journal, The Gold Medal of the Conjoint Membership in Orthodontics Examination of the Royal College of Surgeons of Edinburgh and the College of Dental Surgeons of Hong Kong 2007 – Non-surgical orthodontic treatment of anterior open bite. 2009, 6: 103-107. |
| Lei W.Y., Rabie A.B.M. and Wong R.W.K., Repair of a defect following the removal of an impacted maxillary canine by orthodontic tooth movement: a case report, The Cases Journal 2010. 2010, 3: 62. |
| Leung F.Y.C., Rabie A.B.M. and Wong R.W.K., Osteoporosis, Osteonecrosis and Orthodontics, World Journal of Orthodontics . 2009, 10: 261-271. |
| Li L., Wong R.W.K. and Rabie A.B.M., Oral Presentation: An anti-inflammatory and analgesic ointment for the relief of temporomandibular joint and masticatory muscle pain , 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p15 (OP024). |
| Li L.C.F., Wong R.W.K. and Rabie A.B.M., Clinical Effect of a Topical Herbal Ointment on Pain in Temporomandibular Disorders: A Randomized Placebo-controlled Trial, Journal of Alternative and Complementary Medicine 2009. 2009, 15 No.12: 1311-1317. |
| Li L.C.F., Rabie A.B.M. and Wong R.W.K., Management of Severe Class II Malocclusion with Sequential Fixed Functional and Orthodontic Appliances: A Case for MOrhRCSEd Examination, Singapore Dental Journal. 2010, 31 No.1: 30-35. |
| Li Q.F., Wong R.W.K. and Rabie A.B.M., Gene therapy: an alternative strategy for correction of severe craniofacial deformities, Hong Kong Dental Journal. 2010, 7: 8-13. |
| Ling J.Y.K. and Wong R.W.K., Incisor Winging in Chinese, The Open Anthropology Journal 2010. 2010, 3: 8-11. |
| Ling J.Y.K. and Wong R.W.K., Molar cusps in Southern Chinese, The Open Anthropology Journal 2010. 2010, 3: 16-19. |
| MacLaine J., Rabie A.B.M., Blechman A. and Wong R.W.K., Does orthodontic tooth movement cause an elevation in the systemic inflammatory markers? , 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p151 (SP476). |
| Mok C.W. and Wong R.W.K., Self correction of anterior crossbite: a case report, Cases Journal (Open Access) . 2009, 2: 6967. |
| Mok W., Wong R.W.K. and Hagg E.U.O., Orthodontic treatment planning through spreadsheet programme, Dental Asia July/August 2009. 2009, 43-46. |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia . 2010, March/April 2010: 21-27. |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia November/December 2009. 2009, 24-29. |
| O'Connor D.J., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Resveratrol Inhibits Periodontal Pathogens In Vitro, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Porbunderwalla R., Wong R.W.K. and Rabie A.B.M., Tensile stress on the expression of Sox 9 and subsequent release of Type II collagen in a synchondrosis, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p28 (HP006). |
| Rabie A.B.M., Leung F.Y.C., Wong R.W.K. and Yang Y., A Bag of Tricks: Varying Timing of Extraction Facilitates Midline Correction, The Philippine Journal of Orthodontics. 2009, 8: 1-5. |
| Rizk A...E.L...S., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Transfection of Dental Pulp Stem Cells Using Electro-poration , The 7th International Orthodontic Congress (IOC) World Federation of Orthodontists, Sydney – Australia, 6-9 February, 2010. |
| Seneviratne C.J., Samaranayake L.P., Wong R.W.K. and Hagg E.U.O., Antimicrobial activity of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study.Int J Oral Maxillofac Surg. 2010 Apr 23, 2010. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of Vascular Endothelial Growth Factor (VEGF) on Osteoblastic Cells in vitro, Houston Poster Award - the 86th Congress of the European Orthodontic Society, Portoroz, Slovenia, June 15-19, 2010. 2010. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Vascular Endothelial Growth Factor (VEGF) on Osteoblastic Cell vitro, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Vascular Endothelial Growth Factor on MC3T3-E1 Cell Line, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Thongdee P., Rabie A.B.M. and Wong R.W.K., Poster Presentations: Use of AN ER, CR:YSGG, Waterlase Laser in Orthodontic Patients with Gingival Overgrowth, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Tsui V.W.K., Alkhal H.A., Hou H.M., Wong R.W.K. and Rabie A.B.M., The modified two-by-one fixed orthodontic appliance for bodily movement of canine: a case report, Cases Journal 2009. 2009, 2: 211. |
| Veerawattanatigul A., Wong R.W.K. and Rabie A.B.M., Poster Presentations: The Quercetin Effect on SOX9 and Collagen II Expressions in Spheno-Occipital Synchondroses, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Verayannont P., Hagg E.U.O., Wong R.W.K., McGrath C.P.J. and Yeung S., Maxillary growth during puberty determined by the implant method compared with cephalometry, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p70 (SP119). |
| Wangwarunyoo K., Wong R.W.K. and Rabie A.B.M., Poster Presentations: Effect of Quercetin on Expression of BMP2 and PTHRP in Mice Spheno-Occipital Synchondroses, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Wey M.C., Wu C.L., Wong R.W.K., Zamri R. and Hagg E.U.O., Posterior Teeth Mesialization With Mini-implants In An Oligodontia Patient, Malaysian Dental Journal. 2009, 29(2): 113-118. |
| Wiechmann D., Wong R.W.K. and Hagg E.U.O., Orthodontic Treatment using Incognito; new lingual orthodontic appliance applicating CAD/CAM, Journal of Orthodontic Practice (Japanese version). 2009, 83-90. |
| Wong R.W.K., Orthodontic treatment at puberty, Press Interview – Sun . 2010. |
| Wong R.W.K., 9th Chinese Orthodontic Conference (COS), Sanya, China, 2-5 November, 2009 . 2009. |
| Wong R.W.K., An Editorial Board Member (starting 2009), Journal of Bioanalysis & Biomedicine (Open Access) . 2009. |
| Wong R.W.K., An Editorial Board Member (starting 2010) , Journal of Tissue Science & Engineering (Open Access) . 2010. |
| Wong R.W.K., An Editorial Board Member (starting 2010), Asian Pacific Orthodontic Society . 2010. |
| Wong R.W.K., Hagg E.U.O., Samaranayake L.P., Yuen M.K.Z., Seneviratne C.J. and Kao R., Antimicrobial activities of Chinese medicine herbs against common bacteria in oral biofilm. A pilot study, International Journal of Oral & Maxillofacial Surgery. 2010, 39: 599-605. |
| Wong R.W.K., Basic concept for Orthodontics treatment., Press Interview – Ming Pao . 2009. |
| Wong R.W.K., Chairman of Session in Oral Presentation, 1st Symposium on Evidence-based Integrative Dentistry – Faculty of Dentistry, The University of Hong Kong (HKU). The Prince Philip Dental Hospital, Faculty of Dentistry, University of Hong Kong, 11 December, 2009. 2009. |
| Wong R.W.K., Conference Organization of 24th IADR-SEA Annual Scientific Meeting Abstract Reviewer. 2010. |
| Wong R.W.K., Conference Organizer , 1st Symposium on Evidence-based Integrative Dentistry – Faculty of Dentistry, The University of Hong Kong (HKU). The Prince Philip Dental Hospital, Faculty of Dentistry, University of Hong Kong, 11 December, 2009. 2009. |
| Wong R.W.K., Congress Organizing Committee in IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010 . 2010. |
| Wong R.W.K., Dental Anthropology in Chinese, The Open Anthropology Journal 2010. 2010, 3: 1. |
| Wong R.W.K., Edible Phytochemicals for Bone Engineering, Invited speaker in BIT’s 3rd World Congress of Industrial Biotechnology 2010, Dalian, China, 25-27 July, 2010 . 2010. |
| Wong R.W.K. and Rabie A.B.M., Effect of Buguzhi (Psoralea corylifolia fruit) Extract on Bone Formation, Phytotherapy Research (online in Wiley InterScience). 2009. |
| Wong R.W.K. and Rabie A.B.M., Effect of Genistin on Bone Formation, Frontiers in Bioscience . 2010, E2: 764-770. |
| Wong R.W.K., Executive Associate Editor (starting 2009), Executive Editor, Hong Kong Dental Journal . 2009. |
| Wong R.W.K. and Rabie A.B.M., Grapefruit Flavonoids: naringin and naringinin, Flavonoids: Biosynthesis, Biological Effects and Dietary Sources. Nova Science Publishers, Inc. Nova publishers, 2009. |
| Wong R.W.K., Guest Editor for Special Issue: Dental Anthropology in Chinese, The Open Anthropology Journal. 2010, 3: 1. |
| Wong R.W.K., Interceptive Orthodontics, Invited Speaker in 1st Hong Kong International Dental Expo And Symposium (HKIDEAS) – Hong Kong Convention and Exhibition Centre, 18-20 June, 2010. 2010. |
| Wong R.W.K., Invited Judge for Poster Presentation, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology,16-17 November, 2009. 2009. |
| Wong R.W.K., Invited Session Chair of Oral Presentation, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. 2009. |
| Wong R.W.K., Invited Speaker in the HKU TCM Workshop 2010, 13 July, 2010 , Oral Presentation: TCM for Bong Generation. 2010. |
| Wong R.W.K., Invited editor of special Issue on Dental Anthropology of Chinese, The Open Anthropology Journal . 2009. |
| Wong R.W.K., Journal Reviewer (starting 2009) , Microbiology Indonesia . 2009. |
| Wong R.W.K., Journal Reviewer (starting 2009), Journal of Applied Oral Science . 2009. |
| Wong R.W.K., Journal Reviewer (starting 2010) , Journal of Investigative and Clinical Dentistry . 2010. |
| Wong R.W.K., Journal Reviewer (starting 2010), BMC Oral Health . 2010. |
| Wong R.W.K., Journal Reviewer (starting 2010), European Journal of Histochemistry . 2010. |
| Wong R.W.K., Journal Reviewer (starting 2010), Journal of Orofacial Pain . 2010. |
| Wong R.W.K., Journal Reviewer (starting 2010), Journal of Pediatric Biochemistry . 2010. |
| Wong R.W.K., Journal Reviewer (starting 2010), Medical Principles and Practice . 2010. |
| Wong R.W.K., Journal Reviewer (starting 2010), Medical Science Monitor . 2010. |
| Wong R.W.K., Journal Reviewer (starting 2010), Molecules . 2010. |
| Wong R.W.K., Lingual orthodontic appliances – the CAD/CAM approach, Winter Scientific Meeting: Advances and High-tech Dentistry 2009, Jointly organized by The College of Dental Surgeons of Hong Kong and Faulty of Dentistry, University of Hong Kong, 15 November, 2009. |
| Wong R.W.K., Member of Credentials Committee 2010, the World Federation of Orthodontists . 2010. |
| Wong R.W.K., Member of Specialty Board in Orthodontics, 01/2010-12/2010. 2010. |
| Wong R.W.K., Member of WFO Council, World Federation of Orthodontists, 2010-2015. 2010. |
| Wong R.W.K. and Young F.Y.F., Naringin and its Aglycone, Research and Managements (Hong Kong), Comprehensive Bioactive National Products Vol 2: Efficacy, Safety & Clinical Evaluation (Part 1) 2010. Studium Press LLC, 2010. |
| Wong R.W.K. and Rabie A.B.M., Oral Presentation: In vivo and in vitro effects of phytoestrogen Genistin on Bone Formation, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. 2009. |
| Wong R.W.K. and Rabie A.B.M., Oral Presentation: In vivo and in vitro effects of phytoestrogen Genistin on Bone Formation, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. |
| Wong R.W.K., Oral Presentation: Lingual orthodontic appliances – the CAD/CAM approach, Winter Scientific Meeting: Advances and High-tech Dentistry 2009, Jointly organized by The College of Dental Surgeons of Hong Kong and Faulty of Dentistry, University of Hong Kong, 15 November, 2009. . 2009. |
| Wong R.W.K., Oral Presentation: Phytochemicals for Bone Regeneration, BIT’s 7th Annual congress of International Drug Discovery Science and Technology (IDDST 2009), Shanghai, China, 22-25 October, 2009. 2009. |
| Wong R.W.K., Oral Presentation: TCM and Bone Regeneration, 1st Symposium on Evidence-based Integrative Dentistry, Faculty of Dentistry, The University of Hong Kong (HKU). The Prince Philip Dental Hospital, Faculty of Dentistry University of Hong Kong, 11 December, 2009. 2009. |
| Wong R.W.K., Oral Presentation: The Correlation between Chronological Age and Hand-wrist Skeletal Maturity Indicators in Southern Chinese, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Wong R.W.K., Orthodontic treatment at puberty, Press Interview – Oriental Daily News . 2010. |
| Wong R.W.K., Orthodontic treatment at puberty, Press Interview – Metrodaily . 2010. |
| Wong R.W.K., Orthodontic treatment at puberty, Press Interview – Smart Parents Magazine . 2010. |
| Wong R.W.K. and Rabie A.B.M., Phytochemicals for Bone Regeneration, Biomaterials for Bone Regenerative Medicine – Trans Tech Publications Inc. Materials Science Foundations (monograph series) . 2010, 62: 81-106. |
| Wong R.W.K., TCM and Periodontal Diseases, Apple Daily 2009. 2009. |
| Wong R.W.K., TMD and Orthodontics , Oral Health and Science Seminars. 20 January, 2010. 2010. |
| Wong R.W.K., The Correlation between Chronological Age and Hand-wrist Skeletal Maturity Indicators in Southern Chinese, Invited Speaker in IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau April 11-16, 2010. 2010. |
| Wong R.W.K., The Federation of Medical Societies of Hong Kong, Director 06/2009-05/2010. 2010. |
| Wong R.W.K., The Response of the Spheno-Occipital Synchondrosis to Mechanical Stress, The 7th International Orthodontic Congress (IOC) World Federation of Orthodontists, Sydney – Australia, 6-9 February, 2010. |
| Wong R.W.K., Alkhal H.A. and Rabie A.B.M., Use of cervical vertebral maturation to determine skeletal age, American Journal of Orthodontics & Dentofacial Orthopedics. 2009, 136: 484.e1-484.e6. |
| Wong R.W.K., 中藥與口腔醫學糸列 - 中藥補牙骨, Mingpao Weekly 2010.01.09. 2010, 80-82. |
| Wong R.W.K., 中藥與口腔醫學糸列 - 中藥補牙骨, Press Interview - Mingpao Weekly, 2010.01.09. 2010. |
| Wu A., McGrath C.P.J., Wong R.W.K., Wiechmann D. and Rabie A.B.M., Pain and daily oral impact and satisfaction of patients treated with labial and lingual fixed appliances, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, . 2009, p18 (OP033). |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath C.P.J., Comprehensive Cephalometric Analyses of 10 to 14-Year Old Southern Chinese, The Open Anthropology Journal 2010. 2010, 3: 85-95. |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath M., Poster Presentations: Sagittal Occlusal Analysis of 12-year old Southern Chinese Children, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Xue F., Rabie A.B.M. and Wong R.W.K., Poster Presentation: Association analyses of chromosome 1p36 SNPs identify new positional candidate genes for mandibular prognathism, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November, 2009. |
| Yang Y., Chin A., Tan Y.Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of VEGF and medicinal herb SM on bone cells, Honourable Mention Award of poster presentation at the 1st Hong Kong International Dental Expo and Symposium (HKIDEAS), Hong Kong, 18-20 June 2010. 2010. |
| Yang Y., Tan Y.Y., Chin A., Wong R.W.K. and Rabie A.B.M., Oral Presentation: Effects of Vascular Endothelial Growth Factor and Danshen on Osteoblastic Cell Activities, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Yang Y., Tan Y.Y., Chin A., Chai L., Wong R.W.K. and Rabie A.B.M., Oral Presentation: Effects of Vascular Endothelial Growth Factor (VEGF) and Medicinal Herb Salvia Miltiorrhiza (SM) on Osteoblastic Cell Activities, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Yang Y., Tan Y.Y., Chin A., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of VEGF and Medicinal Herb SM on bone cells, 1st Hong Kong International Dental Expo And Symposium (HKIDEAS) – Hong Kong Convention and Exhibition Centre, 18-20 June. 2010. |
| Yuen K., Wong R.W.K., Hagg E.U.O. and Samaranayake L.P., Antimicrobial activities of forty traditional Chinese medicines on oral bacteria: A experimental study, 85th Congress of the European Orthodontic Society in Helsinki, Finland, 10 – 14 June, 2009. p152 (SP480). |
| Yugaraj P., Zuo P., Rabie A.B.M. and Wong R.W.K., Poster Presentations: In Vitro Differentiation of Dental Pulp Stem Cells into the Chondrogenic Route, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Zhou W., Rabie A.B.M., Wong R.W.K. and Tang B., Nanocoating of Montmorillonite/Mg-β-Tricalcium Phosphate on Orthodontic Titanium Miniscrews, 3rd IEEE International NanoElectronics Conference (INEC). 2010, BC211. |
| Zhu S., Yang Y. and Wong R.W.K., Poster Presentation: A Comparative Study on the Alignment Efficiency of a Lingual System, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Zhu S.J., Yang Y. and Wong R.W.K., Poster Presentation: A Comparative Study on the Alignment Efficiency between Incognito Lingual System and Labial Straight Wire Appliance, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Researcher : Wu D |
| List of Research Outputs |
| Wu D., Lo E.C.M., Zhang Y. and Chu C.H., Oral health and hygiene status of Hong Kong kindergarten children, Journal of Dental Research. 2009, 88 (Spec Iss B): 310 (PAPF/APR). |
| Researcher : Wu JYC |
| List of Research Outputs |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath C.P.J., Comprehensive Cephalometric Analyses of 10 to 14-Year Old Southern Chinese, The Open Anthropology Journal 2010. 2010, 3: 85-95. |
| Wu J.Y.C., Hagg E.U.O., Wong R.W.K. and McGrath M., Poster Presentations: Sagittal Occlusal Analysis of 12-year old Southern Chinese Children, IOS Mid Year Convention 2010 – Beyond Boundaries Compendium of Scientific Deliberations Vol 1-2010 Hong Kong & Macau, April 11-16, 2010. |
| Researcher : Xiao Y |
| List of Research Outputs |
| Lo E.C.M., Xiao Y., Chu C.H. and Liang R., Preventing fissure caries by sealants and fluorides - 30-month results, Journal of Dental Research. 2009, 88 (Spec Iss C): 127 (WCPD). |
| Researcher : Yang Y |
| Project Title: | Effects and signal pathway of vascular endothelial growth factor (VEGF) on osteoblastic cell line of MC3T3-E1 in vitro |
| Investigator(s): | Yang Y, Rabie ABM, Wong RWK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 06/2008 |
| Abstract: |
| Alveolar bone defects caused by trauma, bone pathology, early loss of teeth or during extraction of buccally placed teeth, require restoration. Availability of a broad, well-rounded alveolar ridge is essential for the success of orthodontic tooth movement. Wound healing, tissue regeneration and bone healing are biological events that are strictly dependent on the rapid ingrowth of new capillary blood vessels, a process termed “Angiogenesis” (Folkman and Cotran 1976; Folkman and Klagsburn 1987). It was stated that in healing of bone grafts, a significant correlation exists between the temporal sequence of vascularization and the eventual viability and incorporation of the graft (Rabie, 1997). Ferrara and Davis-Smyth examined vascular endothelial growth factor (VEGF) which was found to promote the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta and human umbilical vein. It has been found that VEGF exerts a number of different effects on the vascular endothelium and endothelial cells. These effects include changes in endothelial cell morphology, cytoskeleton alterations, and stimulation of endothelial cell migration and growth. However, the effects and signal pathway of VEGF on bone cells remain unclear. Thus, the objective of the present study is to investigate whether VEGF can increase osteoblasts’ activities in vitro and to investigate the signal pathway of VEGF on osteoblasts. |
| Project Title: | 7th International Orthodontic Congress What Makes Teeth Move Orthodontically? |
| Investigator(s): | Yang Y |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 02/2010 |
| Completion Date: | 02/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Chin A., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Medicinal Herb Salvia Miltiorrhiza on Osteoblastic Cell Line of MC3T3-E1, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Chin A., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Salvia Miltiorrhiza on Osteoblastic Cell Line of MC3T3-E1, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Rabie A.B.M., Leung F.Y.C., Wong R.W.K. and Yang Y., A Bag of Tricks: Varying Timing of Extraction Facilitates Midline Correction, The Philippine Journal of Orthodontics. 2009, 8: 1-5. |
| Rabie A.B.M. and Yang Y., Perio-ortho conjoint treatment of periodontally compromised patients, Chinese Journal of Orthodontics. 2009, 16: 181-183. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of Vascular Endothelial Growth Factor (VEGF) on Osteoblastic Cells in vitro, Houston Poster Award - the 86th Congress of the European Orthodontic Society, Portoroz, Slovenia, June 15-19, 2010. 2010. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Vascular Endothelial Growth Factor (VEGF) on Osteoblastic Cell vitro, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Tan Y.Y., Yang Y., Chai L., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of Vascular Endothelial Growth Factor on MC3T3-E1 Cell Line, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Yang Y., An expert report on a case seeking legal aid, 2009. |
| Yang Y., Chin A., Tan Y.Y., Chai L., Wong R.W.K. and Rabie A.B.M., Effects of VEGF and medicinal herb SM on bone cells, Honourable Mention Award of poster presentation at the 1st Hong Kong International Dental Expo and Symposium (HKIDEAS), Hong Kong, 18-20 June 2010. 2010. |
| Yang Y., Electronic study models-a new mode of approved records, Advances in High-tech Dentistry 2009 Continuing Education, Jointly Organized by the College of Dental Surgeons of Hong Kong and Faculty of Dentistry HKU, 15 Nov. 2009. |
| Yang Y., Yang Y., Li X.T., Cui L., Fu M.K., Rabie A.B.M. and Zhang D., Functional analysis of core binding factor a1 and its relationship with related genes expressed by human periodontal ligament cells exposed to mechanical stress., European Journal of Orthodontics. 2010, Epub ahead of print. |
| Yang Y., Tan Y.Y., Chin A., Wong R.W.K. and Rabie A.B.M., Oral Presentation: Effects of Vascular Endothelial Growth Factor and Danshen on Osteoblastic Cell Activities, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Yang Y., Tan Y.Y., Chin A., Chai L., Wong R.W.K. and Rabie A.B.M., Oral Presentation: Effects of Vascular Endothelial Growth Factor (VEGF) and Medicinal Herb Salvia Miltiorrhiza (SM) on Osteoblastic Cell Activities, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Yang Y., Oral Presentation: Electronic study models-a new mode of approved records., Advances in High-tech Dentistry 2009 Continuing Education, Jointly Organized by the College of Dental Surgeons of Hong Kong and Faculty of Dentistry HKU, 15 Nov. 2009. |
| Yang Y., Oral Presentation: Preliminary Study on non-contact incisor relationship secondary to condylar resorption., Beyond Boundaries 2010, The Indian Orthodontic Society Mid-Year Convention, 13 April. 2010. |
| Yang Y., Oral Presentation: What make teeth move orthodontically?, The 7th International Orthodontic Congress, Sydney, Australia, 5-10 Feb. 2010. |
| Yang Y., Wong H.M. and Chai L., Poster Presentation: Attitudes of BDSI and BDSII Students to Self-assessment of Performance in PBL Tutorials, The 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 Nov. 2009. |
| Yang Y., Tan Y.Y., Chin A., Wong R.W.K. and Rabie A.B.M., Poster Presentation: Effects of VEGF and Medicinal Herb SM on bone cells, 1st Hong Kong International Dental Expo And Symposium (HKIDEAS) – Hong Kong Convention and Exhibition Centre, 18-20 June. 2010. |
| Yang Y., Reviewer of Journal of Investigative and Clinical Dentistry, 2009. |
| Yang Y., What make teeth move orthodontically?, The 7th International Orthodontic Congress, Sydney, Australia, 5-10 Feb. 2010. |
| Zhu S., Yang Y. and Wong R.W.K., Poster Presentation: A Comparative Study on the Alignment Efficiency of a Lingual System, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Zhu S.J., Yang Y. and Wong R.W.K., Poster Presentation: A Comparative Study on the Alignment Efficiency between Incognito Lingual System and Labial Straight Wire Appliance, Conjoint Scientific Sessions 2009, The University of Hong Kong – Peking University School of Stomatology, 16-17 November. 2009. |
| Researcher : Yau JYY |
| List of Research Outputs |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Pseudomonas aeruginosa inhibits in-vitro Candida biofilm development, BMC Microbiology. 2010, 10: 125 (pages 1-9). |
| Bandara H.M.H.N., Yau J.Y.Y., Watt R.M., Jin L.J. and Samaranayake L.P., Escherichia coli and its lipopolysaccharide modulate in vitro Candida biofilm formation, Journal of Medical Microbiology. 2009, 58: 1623-1631. |
| Samaranayake Y.H., Yau J.Y.Y., THEIN Z.M., Jayatilake J.A.M.S., Yeung S.K.W. and Samaranayake L.P., The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its imact on the colonization traits of Candida glabrata, Medical Mycology. 2010, 1-10. |
| Researcher : Yau YY |
| List of Research Outputs |
| Lo J., Yau Y.Y. and Cheung L.K., The use of skeletal of skeletal scintigraphy in the assessment of mandibular asymmetry, Conjoint Scientific Meeting of HKU and Peking University . 2009. |
| Researcher : Yeung RWK |
| Project Title: | Prospective study on arthrocentesis |
| Investigator(s): | Yeung RWK, Cheung LK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Low Budget High Impact Programme |
| Start Date: | 11/2001 |
| Abstract: |
| To determine the short and long term benefit of TMJ arthrocentesis in closed locking joints; to compare the sodium hyaluronate and steriod and normal saline in TMJ arthrocentesis; to determine the intraarticular joint pressure during closed lock and the pressure changes following treatment; to determine the biochemical markers in the closed lock joints - e.g. cytokines. |
| Researcher : Yeung SKW |
| List of Research Outputs |
| Samaranayake Y.H., Yau J.Y.Y., THEIN Z.M., Jayatilake J.A.M.S., Yeung S.K.W. and Samaranayake L.P., The post-antifungal effect (PAFE) of amphotericin B, nystatin, ketoconazole and 5-fluorocytosine and its imact on the colonization traits of Candida glabrata, Medical Mycology. 2010, 1-10. |
| Researcher : Yip HK |
| Project Title: | Local delivery of hMSCs and re-constituted collagen for rapid bone regeneration. |
| Investigator(s): | Yip HK, Chan GCF, Chan BP, Chan D |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2006 |
| Abstract: |
| Implant dentistry has been successfully practised for over two decades and its efficacy is proven by long-term clinical trials (Esposito et al., 2005). But problems remain such as the loss of alveolar bone and supportive structures in edentulous areas or loosening of the implant due to suboptimal integration of the implant with the supporting structures. The use of multipotential stem cells to improve the formation of supportive structures is therefore a very appealing approach. Stem cell based composite with collagen scaffolds is becoming a critical part in the bone generation and osseointegration process after the implant surgery. |
| Project Title: | Oral biofilms formed by salivary microorganisms from patients with high and low risk of caries. |
| Investigator(s): | Yip HK, Fang HHP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 10/2007 |
| Abstract: |
| The objective is to compare oral biofilms formed in an in vitro mouth system from salivary microorganisms taken from patients with high and low risk of caries . |
| Project Title: | Study of the fate of mesenchymal stem cells in bone regeneration around titanium implants in mice. |
| Investigator(s): | Yip HK, Chan BP, Chan D, Chan GCF, Cheung KMC |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 09/2008 |
| Abstract: |
| (1) To perform cellular, molecular, biochemical and biomedical in-vitro and in-vivo biological studies in order to assess the bone tissue formed around titanium implants; (2) To study the fate of GFP+MSC in the regeneration of bone around titanium implants in mouse long bone. |
| Project Title: | Studying mesenchymal stem cell differentiation around titanium implant surface using microscopy and quantitative proteomic techniques in mice |
| Investigator(s): | Yip HK, Chan BP |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2008 |
| Abstract: |
| The objective of the proposed project is to identify the protein machineries that regulate mesenchymal stem cell differentiation around titanium implant using molecular imaging and quantitative proteomic techniques. This novel technical approach will map the molecular mechanisms involved in nuclear reprogramming during bone differentiation. To achieve this general aim, the proposed project will employ the use of a combination of molecular imaging and quantitative proteomic technique “SILAC” (see below) in the study of four main aspects of bone differentiation around titanium implant surface: 1. To study the changes in the organization of cell nucleus during in vitro osteoblast differentiation around titanium implant using both fixed cell and live cell imaging. 2. To identify and quantitate, using quantitative proteomics, the proteins synthesized and targeted to the cell nucleus during defined time windows during the in vitro osteoblast differentiation of a mesenchymal stem cell line around titanium implant surface. 3. To study the changes in protein compositions of selected protein machineries and cell organelles during in vivo osteoblast differentiation around titanium implant surface during tissue engineering. This study will derive models of the nuclear processes involved in mesenchymal stem cell differentiation around titanium implant surface based on a synthesis of in vivo and proteomic data. |
| List of Research Outputs |
| Chu C.H., Mei L., Lo E.C.M., Seneviratne C.J. and Yip H.K., Effects of silver diamine fluoride on mono-species cariogenic biofilms, Journal of Dental Research. 2009, 88 (Spec Iss B): 363 (PAPF/APR). |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., Effects of silver-diamine fluoride on caries-like lesion and cariogenic biofilm, Journal of Dental Research. 2009, 88 (Spec Iss B): 40 (PAPF/APR). |
| Mei L., Seneviratne C.J., Yip H.K., Chu C.H. and Lo E.C.M., International Association for Dental Research / Unilever Hatton Travel Award for M.Lei (co-author), 23nd Annual Scientific Meeting, Southeast Asian Division of International Association for Dental Research 2009. . 2009. |
| Researcher : Yiu CKY |
| Project Title: | Controlled release of chlorhexidine from dental adhesive resin |
| Investigator(s): | Yiu CKY, King NM, Hiraishi N |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 07/2007 |
| Completion Date: | 06/2010 |
| Abstract: |
| Objectives: (1) To examine the effect of resin hydrophilicity and chlorhexidine loading on the release of chlorhexidine from dental adhesive resin. (2) To examine the effect of water storage and chlorhexidine release on ultimate tensile strength of dental adhesive resin. (3) To examine the effect of resin hydrophilicity and chlorhexidine loading in dental adhesive resin on microtensile bond strength to dentin. The Null Hypothesis: (1) Resin hydrophilicity and chlorhexidine loading have no effect on the release of chlorhexidine from dental adhesive resin. (2) Water storage and chlorhexidine release have no effect on ultimate tensile strength of dental adhesive resin. (3) Resin hydrophilicity and chlorhexidine loading in dental adhesive resin have no effect on microtensile bond strength to dentin. Key issues and problems: 1. Owing to the increased demand of patients for esthetic restorations, tooth-colored resin composites are becoming the primary restorative materials to replace tooth structure loss through dental caries and trauma. Dentin adhesives are used to bond resin composites to tooth surfaces by forming hybrid layer at the bonded interface. The quality and stability of the hybrid layer, which consists of adhesive and dentin collagen, are of prime importance to the success of bonded restorations. Over the years, multi-step adhesive systems have been simplified for easier clinical application. A major drawback of these simplified dentin adhesives is their increased hydrophilicity and permeability, which promote fluid entry, leakage and bond failure. Recent clinical studies have shown loss of resin-dentin bond durability over time. A potential cause of bond degradation is the breakdown of the demineralized collagen matrix in dentin by host-derived matrix metalloproteinases (MMPs). 2. Chlorhexidine (CHX) has been widely used in clinical dentistry. Apart from being an oral antimicrobial agent, it also functions as a potent MMP inhibitor. It has been reported that CHX, even in very low concentrations, completely inhibited the collagenolytic activity of MMP in mineralized dentin. Thus, the incorporation of CHX into dentin adhesives may be a potential method to improve the durability of resin-dentin bond. Very few studies have measured the amount of CHX release from dental adhesive resin as a function of time. Consequently, we plan to examine the release rate of CHX from experimental dental adhesive resin. Incorporation of chlorhexidine within dental adhesive resin may affect the handling and mechanical properties of adhesive resin. Thus, we will also evaluate the effect CHX on mechanical properties of dental adhesive resin and determine whether such release can affect the microtensile bond strength to dentin. |
| Project Title: | Research Output Prize (Faculty of Dentistry) |
| Investigator(s): | Yiu CKY |
| Department: | Dental Faculty |
| Source(s) of Funding: | Research Output Prize (in Faculty) |
| Start Date: | 11/2007 |
| Abstract: |
| The Research Output Prize accords recognition to an author (or team of authors) of a single research output published or created in the preceding calendar year. Faculties are free to determine what form of research output best represents their research achievement and how it should be selected. |
| Project Title: | Characterization and biodegradation of the resin-dentin interface in restored teeth |
| Investigator(s): | Yiu CKY |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2008 |
| Completion Date: | 12/2009 |
| Abstract: |
| To assess the effect of salivary cholesterol esterases (CEs), matrix metalloproteinases (MMPs) and cysteine proteinases (CPs) on the microtensile bond strength (MTBS) and ultra-structural morphology of resin-dentin interface in an artificial saliva system, testing both conventional and simplified systems Null hypothesis for objective 1 Exogenous cholesterol esterases (CEs), matrix metalloproteinases (MMPs) and cysteine proteinases (CPs) have no effect on the microtensile bond strength (MTBS) and ultra-structural morphology of the resin-dentin interface; to examine the effect of MMP inhibitors on durability of resin-dentin bond. Null hypothesis for objective 2 MMP inhibitors have no effect on resin-dentin bond durability. |
| Project Title: | Effect of ethanol wet-bonding on resin-dentin bond |
| Investigator(s): | Yiu CKY, King NM, Hiraishi N |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 04/2009 |
| Abstract: |
| Objectives (1) To examine the effect of ethanol wet-bonding on the bonding efficacy of hydrophobic adhesive to caries-affected dentin. (2) To examine the effect of reducing application time of ethanol wet-bonding on the bonding efficacy of hydrophobic adhesive to dentin. Null hypotheses (1) Ethanol wet-bonding does not alter the bond strength of hydrophobic adhesive to caries-affected dentin. (2) Reducing the application time on ethanol wet-bonding technique has no effect on the bond strength of hydrophobic adhesive to dentin. Key issues and problems The use of tooth-colored resin composite to restore traumatized and carious teeth has increased recently, as a result of consumer demands for esthetic restorations. The bonding of resin composite to dentin is achieved using dentin adhesives which form a hybrid layer at the bonded interface. The quality and stability of this hybrid layer, which consists of adhesive resin and dentin collagen, are imperative to the longevity of the resin composite restorations. Over the years, multi-step adhesive systems have been simplified for easier clinical application. A major drawback of these simplified dentin adhesives is their increased hydrophilicity and permeability, which promote fluid entry, leakage and bond failure. To improve the durability of resin-dentin bond, it has been proposed that future dentin adhesives be rendered less hydrophilic (Tay and Pashley 2003). Ethanol wet-bonding has recently been proposed as a bonding technique to more effectively infiltrate hydrophobic monomers into acid-etched dentin for a more durable bond as compared to traditional approaches (Nishitani et al., 2006, Pashley et al., 2007). In this technique, acid-etched dentin is made less hydrophilic, so that it is compatible with hydrophobic resin monomers. Water in acid-etched dentin is slowly replaced by an ascending concentration of ethanol (50, 70, 80, 95 and 100% for three times for 30 s each), avoiding collapse of the interfibrillar spaces within the collagen matrix. Preservation of these spaces enables the ethanol to be replaced with increasing concentration of hydrophobic resin dissolved in ethanol, and finally pure hydrophobic resin. However, all published reports on the ethanol wet-bonding technique have used normal dentin as the substrate, even though caries-affected dentin is often the clinically encountered bonding substrates. Thus, we proposed to examine the effect of ethanol wet-bonding on the bonding efficacy of hydrophobic adhesives on caries-affected dentin. Additionally, the current ethanol wet-bonding technique is quite time-consuming and involves several more application steps than conventional wet bonding protocols. Very few studies have examined the effect of simplifying the ethanol wet-bonding technique on bonding efficacy of hydrophobic adhesives on dentin. Further work should be performed to shorten the dehydration process in dentin before this technique can be recommended for clinical use. References Nishitani Y, Yoshiyama M, Donnelly AM, Agee KA, Sword J, Tay FR, Pashley DH. Effects of resin hydrophilicity on dentin bond strength. Journal of Dental Research 2006; 85: 1016-21. Pashley DH, Tay FR, Carvalho RM, Rueggeberg FA, Agee KA, Carrilho M, Donnelly A, García-Godoy F. From dry bonding to water-wet bonding to ethanol-wet bonding. A review of the interactions between dentin matrix and solvated resins using a macromodel of the hybrid layer. American Journal of Denistry 2007; 20: 7-20. Review. Tay FR, Pashley DH. Dental adhesives of the future. Journal of Adhesive Dentistry 2002; 4: 91-103. Review. |
| Project Title: | 10th Congress of the European Academy of Paediatric Dentistry (EAPD 2010) Health and Oral Health-related Quality of Life among Preschool Children with Cerebral Palsy |
| Investigator(s): | Yiu CKY |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 06/2010 |
| Completion Date: | 06/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Au A.H.O., Hui K.K.K., Lau E.W.S., Tong L.S.M. and Yiu C.K.Y., Guidelines on sedation in pediatric patients, Hong Kong Society of Pediatric Dentistry . 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience - Journal Based Learning and Fundamentals of Dental Research – a pilot programme, the 6th International Conference on PBL in Dentistry. Hong Kong, 2009. |
| Botelho M.G., Lo E.C.M., Dyson J.E., Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., A new final year learning experience-journal based learning and fundamentals of dental research-a pilot programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.26. |
| Bridges S.M., Botelho M.G., Dyson J.E., McGrath C.P.J., Tsang P.C.S. and Yiu C.K.Y., Blended learning and interactive pedagogies: Supporting problem-based learning in dental education, CITE Research Symposium 2010. 2010. |
| Bridges S.M., McGrath C.P.J. and Yiu C.K.Y., Multilingual clinical interactions in Dentistry: A microanalysis, 11th International Pragmatics Conference. Australia, 2009. |
| Bridges S.M., McGrath C.P.J., Yiu C.K.Y. and Cheng S.S., Reassuring' during clinical examinations: Novice and expert talk in dentistry, Journal of Asian Pacific Communication. John Benjamins, 2010, 20:2: 185-206. |
| Bridges S.M., Botelho M.G., Corbet E.F., Dyson J.E., McGrath C.P.J., Yiu C.K.Y. and Ng K.S., Reconceptualizing the Psychosocial Domain of an Integrated PBL Curriculum Design, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.39. |
| Chung C.W., Yiu C.K.Y., King N.M., Hiraishi N. and Tay F.R., Effect of saliva contamination on bond strength of resin luting cements to dentin. , Journal of Dentistry. 2009, 37: 923-31. |
| Corbet E.F., Bridges S.M., McGrath C.P.J., Yiu C.K.Y., Dyson J.E., Botelho M.G. and Cheng S.S., Formative assessment of clinical communication skills, 20th South East Asia Association for Dental Education Annual General Meeting, Chiang Mai, Thailand, November 23-24. 2009, 36. |
| Du R.Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health- and oral health-related quality of life among preschool children with cerebral palsy, Quality of Life Research. 2010, 19: 1367-1371. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Du RY, McGrath C, Yiu CKY, King NM. Health-related and oral health-related quality of life among preschool children with cerebral palsy. Best Poster Presentation , Conjoint scientific sessions – Faculty of Dentistry, the University of Hong –Peking University School of Stomatology, 16-17 November 2009. 2009. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health and oral health-related quality of life among preschool children with Cerebral Palsy, 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Health-related and oral health-related quality of life among preschool children with cerebral palsy. , Conjoint Scientific Session. The University of Hong Kong-Peking University School of Stomatology, 16-17 November, 2009. p.32. |
| Du Y., McGrath C.P.J., Yiu C.K.Y. and King N.M., Oral health in the primary dentition of children with cerebral palsy. , 10th Congress of the European Academy of Pediatric Dentistry. 2010. |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R.C.M., Anti-microbial efficacy of 3.8% silver diamine fluoride and its effect on root dentin., Journal of Endodontics. 2010, 36:: 1026-1029. |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R., Antibacterial effect of experimental chlorhexidine-releasing polymethyl methacrylate-based root canal sealers. , Journal of Endodontics. 2009, 35: 1255-8. |
| Hiraishi N., Yiu C.K.Y., King N.M., Tagami J. and Tay F.R.C.M., Antimicrobial efficacy of 3.8% silver diamine fluoride and its effect on root dentin, Journal of Endodontics. 2010, 36(6): 1026-1029. |
| Hiraishi N., Yiu C.K.Y., King N.M. and Tay F.R.C.M., Effect of chlorhexidine incorporation into a self-etching primer on dentine bond strength of a luting cement., Journal of Dentistry. 2010, 38: 496-502. |
| Kim J.R., Mai S., Carrilho M.R., Yiu C.K.Y., Pashley D.H. and Tay F.R.C.M., An all-in-one adhesive did not etch beyond hybrid layers. , Journal of Dental Research. 2010, 89: 482-487. |
| Kim Y.K., Yiu C.K.Y., Kim J.R., Gu L.S., Kim S.K., Weller R.N., Pashley D.H. and Tay F.R.C.M., Failure of a Glass Ionomer to Remineralize Apatite-depleted Dentin. , Journal of Dental Research. 2010, 89: 230-235. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and Responsiveness of ChineseECOHIS to Dental Treatment, 1st Hong Kong International Dental Expo and Symposium. 2010. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Sensitivity and responsiveness of the Chinese version of the Early Childhood Oral Health Impact Scale (ECOHIS) to dental treatment under general anaesthesia. , Conjoint Scientific Session 2009. The University of Hong Kong-Peking University School of Stomatology, 16-17 Kong November 2009. p.32. |
| Lee H.M.G., McGrath C.P.J., Yiu C.K.Y. and King N.M., Translation and validation of a Chinese language version of the Early Childhood Oral Health Impact Scale (ECOHIS), International Journal of Paediatric Dentistry. 2009, 19: 399-405. |
| Mai S., Kim Y., Kim J., Yiu C.K.Y., Ling J., Pashley D.H. and Tay F.R.C.M., In vitro remineralization of severely compromised bonded dentin., Journal of Dental Research . 2010, 89: 405-410. |
| Yiu C.K.Y., An update of caries preventive strategies in children, Spring Scientific Meeting, The College of Dental Surgeons of Hong Kong. 2010. |
| Yiu C.K.Y., Associate Executive Editor, Hong Kong Dental Journal. 2009. |
| Yiu C.K.Y., Caries preventive strategies in children, Oral Health and Science Seminar. 2010. |
| Yiu C.K.Y., Editorial Advisory Board, Journal of Investigative and Clinical Dentistry. 2009. |
| Yiu C.K.Y., Chung C.W.M., Hiraishi N., King N.M. and Tay F.R., Effect of chlorhexidine concentration on the mechanical properties of dental adhesive resins., Conjoint Scientific Session 2009. The University of Hong Kong-Peking University School of Stomatology, 16-17 November 2009. p.20. |
| Yiu C.K.Y., Bridges S.M. and Cheung R.T.F., Evaluation of Medical Case-based Learning as a Dental Curriculum Enhancement Programme, 6th International Conference on PBL in Dentistry, Hong Kong, 13-15 November 2009. p.21. |
| Yiu C.K.Y., PBL in a time of local and global curriculum reform , 6th International Conference on PBL in Dentistry, 13-15 November 2009, Hong Kong. 2009. |
| Researcher : You DSH |
| List of Research Outputs |
| Chu C.H., Lo E.C.M. and You D.S.H., Clinical diagnosis of fissure caries with conventional and laser-induced fluorescence techniques, Lasers Medical Science. 2010, 25: 355-362. |
| Wong A.H.H., You D.S.H., Chan L.S.Y., Chan W.Y., Cheung A.C.H., Lam K.W., Liu W.Y., Wong W.K., Yeung P.M., Yim P.K. and Yun L., A clinical trial on the effect of modified manual toothbrush and electric toothbrush and electric toothbrush on oral hygiene and gingival health of Hong Kong stroke patients, Community Health Project Report Series. Faculty of Dentistry, The University of Hong Kong, 2010, 165: 1-75. |
| Researcher : You M |
| List of Research Outputs |
| You M., Mo S., Leung W.K. and Watt R.M., Diversity of oral treponemes in patients with chronic periodontitis, Journal of Dental Research. 2009, 88 (Spec Iss B): 377 (PAPF/APR). |
| Researcher : Zee KY |
| List of Research Outputs |
| Chai L., Song Y., Zee K.Y. and Leung W.K., Single nucleotide polymorphisms of complement component 5 and periodontitis, J Periodontal Res. 2010, 45(3): 301-308. |
| Researcher : Zhang C |
| Project Title: | Dentin-pulp complex and periodontal tissues regeneration in root canal perforation by the stem cells from the apical papilla and periodontal ligament. |
| Investigator(s): | Zhang C, Cheung GSP, Jin LJ |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 12/2009 |
| Abstract: |
| Objectives 1. To investigate the ability of the stem cells from apical papilla (SCAPs), periodontal ligament stem cells (PDLSCs) for regeneration of dentin-pulp complex and periodontal tissues together. 2. To explore whether the stem cells from apical papilla (SCAPs) and periodontal ligament stem cells (PDLSCs) have synergic effect on their regeneration abilities. 3. To evaluate the possibility of using SCAPs and PDLSCs for root canal perforation repair. Key issues and problems addressed Recent progress in regenerative medicine has led to a growing interest in the development of regenerative endodontics (1). Regeneration of the dentin-pulp complex and periodontal tissues holds promise for replacement of damaged or missing tooth structures. One potential use of regenerative endodontic therapy might be the repair of root canal perforations. Although many materials have been used over the years, none of the currently available materials fulfill all the ideal requirements. An ideal perforation repair material should possess good sealing ability, be easy to manipulate and place, be compatible with surrounding tissues, and promote tissue regeneration in the site of the perforation. Mineral trioxide aggregate (MTA) is the current material of choice for perforation repair, and has demonstrated good potential for clinical success, but placement of MTA or any other restorative material prevents regeneration of dentin-like hard tissue and periodontal tissues in the perforation area. MTA does not degrade to allow for replacement with natural tissue (2, 3). The presence of a perforation has been identified as one of the most significant negative predictors of treatment outcome for root canal retreatment therapy. Dentin-pulp complex and periodontal ligament-cementum complex have been engineered with human dental pulp stem cells (DPSCs), the stem cells from apical papilla (SACPs) and periodontal ligament stem cells (PDLSCs) respectively, but how to form the periodontal ligament-cementum, and dentin together by tissue engineering techniques remains to be solved (4-6). In some clinical situations such as root canal perforation, external and internal absorption and open apex in immature permanent teeth with apical periodontitis, the regeneration of dentin-pulp complex and periodontal ligament-cementum complex together by tissue engineering techniques might be a new protocol for the clinical management of these cases. Tissue engineering requires three key ingredients, namely stem cells, an appropriate scaffold and growth factors. SCAPs, DPSCs and PDLSCs can be obtained from the impacted third molar, premolar extracted for orthodontic treatment, and supernumerary tooth. These teeth usually discarded after extraction might therefore be a valuable source of human SCAPs, DPSCs and PDLSCs. Banking these teeth, tissues, or stem cells for autologous use is a viable option (7). Ideally, a scaffold should be porous to allow for placement of cells and growth factors, biocompatible with the host tissues, appropriate shape and form to allow for replacement of the lost tissues, and biodegradable leaving no toxic by-products. Natural scaffolds (e.g. collagen) offer good biocompatibility and bioactivity, whereas synthetic scaffolds (e.g. polylactic acid, PLA; or polyglycolic acid, PGA) offer more controlling over the degradation rate and mechanical properties (4, 6). Alginate hydrogel has been used as a scaffold in hard (bone) tissue engineering (8). Thus, a scaffold is far more than a simple lattice to contain cells. From the perspective of clinical applications, it is believed that platelet-rich plasma (PRP) satisfies many of these criteria. PRP is autologous, fairly easy to prepare in a dental setting, rich in growth factors, degradable over time, and forming a 3-dimensional fibrin matrix (9-11). To date, however, no publications have evaluated PRP for scaffold in regenerative endodontic applications. To our knowledge, there has been no study on the repair of root canal perforation by regenerative endodontic approach. This study is to explore the possibility of regeneration of pulp-dentin complex, periodontal ligament-cementum complex together in vivo for perforation repair. |
| List of Research Outputs |
| Li Y., Zhang C. and Wang X., Microbiological study of infected root canals: a review, J Oral Science Res. 2010, 26: 437-439. |
| Ma Z., Xu Z. and Zhang C., Enhanced growth-inhibitory effect of cisplatin by quinacrine in SCC-VII cells in vitro and in vivo, Shan Dong Yi Yao. 2010, 19: 45-46. |
| Wang Q., Zhang S., Wang Y. and Zhang C., The Dominant Bacteria Species in Periapical Endodontic Lesions of Persistent Apical Periodontits, 2009 Conjoint Scientific Sessions: Faculty of Dentistry, The University of Hong Kong - Peking University School of Stomatology. 2009. |
| Wang Y., Cheung G.S.P., Xu X., Zhao S. and Zhang C., The effect of cultured autologous periodontal ligament cells on the healing of delayed autotransplanted dog's teeth, Journal of Endodontics. 2010, 36: 264-267. |
| Yan H., Ma C., Ren M., Yin X., Zhang C., Wei Y. and Dong X., K3 NiTi file negotiation of resinfying root canal and evalulation of its damage, J Nurse Training. 2009, 10: 1899-1900. |
| Yin X., Cheung G.S.P., Zhang C., Masuda Y.M., Kimura Y. and Matsumoto K., Micro-computed tomograhic comparison of nickel-titanium rotary versus traditional instruments in c-shaped root canal system, Journal of Endodontics. 2010, 36: 708-712. |
| Zhang C., Clinical management of clinical failure cases, Fujian Stomatological Association Nov. . 2009. |
| Zhang C., Clinical management of problems in Endodontic treatment, Hong Kong Dental Association Feb.. 2010. |
| Zhang C., Management of two canals in one root, Capital Medical University School of Stomatology June. 2010. |
| Zhang C., Regenerative Endodontics, Peking University School of Stomatology May. 2010. |
| Zhu W., Wang S. and Zhang C., A clinical study on cavities' preparation of subgingival wedge - shape defects using Er: YAG laser, J Modern Stomatol. 2010, 24: 21-23. |
| Zhu X., Wang Q., Yin X. and Zhang C., Preliminary study on Enterococcus faecalis isolated from saliva and root canals, 2nd Meeting of IADR Pan Asia Pacific Federation (PAPF), and 1st Meeting of IADR Asia Pacific Region, 22-24 Sept.. 2009. |
| Zhu X., Wang Q., Yin X. and Zhang C., Prevalence, phenotype and genotype of Enterococcus faecalis isolated in oral-rinse and root-canal samples from patients with periapical lesions after root-canal-treatment (poster presentation), 2009 Conjoint Scientific Sessions: Faculty of Dentistry, The University of Hong Kong - Peking University School of Stomatology. 2009. |
| 馬 ......, 許 振.起. and Zhang C., 奎納克林對順鉑抑制口腔鱗癌細胞系SCC-VII體內外生長作用的影響, Shandong Medical Journal . 2010, 50: R739.8. |
| Researcher : Zhang W |
| List of Research Outputs |
| McGrath C.P.J., Zhang W. and Lo E.C.M., A review of the effectiveness of oral health promotion activities among elderly people, Gerodontology. 2009, 26: 85-96. |
| Zhang W., McGrath C.P.J. and Lo E.C.M., A comparison of root caries diagnosis based on visual-tactile criteria and DIAGNOdent in vivo, Journal of Dentistry. 2009, 37: 509-513. |
| Zhang W., Biochemical modulation and stem cell therapy for irradiated mandible (PhD Thesis - The University of Hong Kong). University of Hong Kong, Hong Kong, 2009, 1-191. |
| Zhang W., Zheng L. and Cheung L.K., Bone regeneration following radiotherapy: an animal model, J Oral Maxillofacial Surg . 2010, In press. |
| Researcher : Zhang W |
| List of Research Outputs |
| McGrath C.P.J., Zhang W. and Lo E.C.M., A review of the effectiveness of oral health promotion activities among elderly people, Gerodontology. 2009, 26: 85-96. |
| Zhang W., McGrath C.P.J. and Lo E.C.M., A comparison of root caries diagnosis based on visual-tactile criteria and DIAGNOdent in vivo, Journal of Dentistry. 2009, 37: 509-513. |
| Zhang W., Biochemical modulation and stem cell therapy for irradiated mandible (PhD Thesis - The University of Hong Kong). University of Hong Kong, Hong Kong, 2009, 1-191. |
| Zhang W., Zheng L. and Cheung L.K., Bone regeneration following radiotherapy: an animal model, J Oral Maxillofacial Surg . 2010, In press. |
| Researcher : Zhang Y |
| List of Research Outputs |
| Wu D., Lo E.C.M., Zhang Y. and Chu C.H., Oral health and hygiene status of Hong Kong kindergarten children, Journal of Dental Research. 2009, 88 (Spec Iss B): 310 (PAPF/APR). |
| Researcher : Zheng J |
| List of Research Outputs |
| McMillan A.S., Wong M.C.M., Zheng J., Luo Y. and Lam C.L.K., Widespread pain symptoms and psychological distress in southern Chinese with orofacial pain, Journal of Oral Rehabilitation. 2010, 37: 2-10. |
| Zheng J., McMillan A.S., Wong M.C.M., Luo Y. and Lam C.L.K., Investigation of factors associated with treatment-seeking in southern Chinese with orofacial pain, Journal of Orofacial Pain. 2010, 24: 181-188. |
| Researcher : Zheng L |
| Project Title: | ISCFS XIII Biennial International Congress ANGIOGENESIS AND OSTEOGENESIS ARE ENHANCED BY CONTINUOUS TRACTION IN RABBIT MANDIBULAR LENGTHENING |
| Investigator(s): | Zheng L |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 09/2009 |
| Completion Date: | 09/2009 |
| Abstract: |
| N/A |
| Project Title: | Roles of costimulatory molecules in pathogenesis and malignant transformation of oral mucosal lesions |
| Investigator(s): | Zheng L, Peng X, Zwahlen RA, Cheung LK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 10/2009 |
| Abstract: |
| Purpose: To assess the role of costimulatory molecules in pathogenesis and malignant transformation of oral mucosal lesions, and compare the expression of B7 family between oral lichen planus (OLP), oral cancers and normal oral mucousal tissues. Key issues and problems: OLP is a common chroinc inflammatory mucosal disease. The pathogenesis is associated with cell-mediated immunological dysfunciton. Histopathological charactristics of OLP demonstrate inflammatory response, such as intense subepithelial infiltration of T-lymphocytes, degeneration of basal keratinocytes and epithelial basement membrane disruption. Oral squamous cell carcinoma (OSCC), the most common cancer of the oral cavity, accounts for over 90% or malignant neoplasms in this area. OLP is the frequent type of oral premalignant lesions and may transform to OSCC. The molecular pathway that controls the pathogenesis and malignant transformation of OLP has not been fully understood. . The activation of native T cells is initiated by assocation of the T-cell recepotr with the major histocompatibility complex(MHC)-peptide complex. Optimal T-cell activation, however, requires a second signal, known as costimulation, which leads to clonal T-cell expansion, cytokine secreton and execution of effector functions. After initial activation, coinhibitory molecules are engaged and conteract T-cell activation. Consequently, a balanced interplay between costimulatory and coinhibitory signals is required to control priming, functional maturation and limitation of the T-cell response at distinct times and locaitons. Lack of costimulation, however, results in the induction of T-cell tolerance leading to prolonged unresponsiveness. Costimulatory molecules, such as B7 family are crucial to T cell activation by controlling the initiation and termination of immune responses. Loss of costimulatory molecules on the cell surface is one of the main escaping mechanisms of tumor cells. Most tumors show low immunogenicity and lack the expression of costimulatory molecules. Some studies have assessed the expression of B7 family in the normal and tumor tissues, however, the role of costimulatory molecules in premalignant lesions such as OLP has not been explored. The B7 family members are classfied into three groups according to their functions. B7-1, B7-H1, B7-H2, B7-H3 and B7-H4 are the crucial members of B7 family, and each with different, although ovelapping, functions controlling the priming, proliferation and maturation of T cells. The efficacy of immune responses directed against target sites, including tumors, is obviously defined by an orchestrated balance between these factors. Increasing knowledge about costimulatory and coinhibitory B7 family members has recently led to the manipulation of these pathways for the treatment of malignant diseases. The present study may provide valuable information and evidence to understand the molecular mechanism of the pathogenesis of OLP and its potential malignant transformation, which may futher lead to a new treatment of blocking the malignant transformation pathway. Main study hypothesis: Alteration of costimulatory molecules may play an important role in the pathogenesis and malignant transformation of lichen planus. Study objectives: 1) To study the expression patterns of the B7-1, B7-H1, B7-H2, B7-H3 and B7-H4 in OLP. 2) To compare the mRNA expression of B7-1, B7-H1, B7-H2, B7-H3 and B7-H4 between OLP, OSCC and normal oral mucousal tissues. |
| List of Research Outputs |
| Cheung L.K., Chua H.D.P., Hariri F., Lo J., Ow A. and Zheng L., Distraction Osteogenesis (Chapter 48), Oral and Maxillofacial Surgery, edited by Lars Andersson, Karl-Erik Kahnberg and M. Anthony Pogrel. Wiley-Blackwell, 2010, 1027 - 1059. |
| Ke J., Cheung L.K. and Zheng L., The best poster award at the 3rd Hiroshima Conference, Hiroshima University. 2009. |
| Liu X.S., Gao Y., Zheng L. and Hua H., New alternative therapy for orofacial localized scleroderma, Oral Surg Oral Med Oral Pathol Oral Radiol Endod . 2010, In press. |
| Ma L., Zheng L., Sham M.H. and Cheung L.K., Effect of nicotine on gene expression of angiogenic and osteogenic factors in a rabbit model of bone regeneration, Journal of Oral and Maxillofacial Surgery. 2010, 68: 777 - 781. |
| Ma L., Sham M.H., Zheng L. and Cheung L.K., Influence of low dose nicotine on bone healing. , J Trauma . 2010, In press. |
| Ma L., Zheng L., Sham M.H. and Cheung L.K., Uncoupled angiogenesis and osteogenesis in nicotine compromised bone healing, J Bone Miner Res . 2010, 25: 1305-13. |
| Wu Z.X., Zheng L., Li Z.B., Yang X.W., Dong Y.J., Zhao Y.F. and Zwahlen R.A., Subapical anterior maxillary segmental osteotomy: a modified surgical approach to treat maxillary protrusion, J Craniofac Surg. 2010, 21: 97-100. |
| Zhang W., Zheng L. and Cheung L.K., Bone regeneration following radiotherapy: an animal model, J Oral Maxillofacial Surg . 2010, In press. |
| Zheng L., (Member of Advisory Board), International Journal of Academic Research. Proqres Publishing Co, 2009. |
| Zheng L., Ma L. and Cheung L.K., Angiogenesis is enhanced by continuous traction in rabbit mandibular distraction osteogenesis, Journal of Cranio-Maxillofacial Surgery. 2009, 37(7): p405-11. |
| Zheng L., Ma L. and Cheung L.K., Angiogenesis is enhanced by high frequency traction during distraction osteogenesis, J Craniomaxillofac Surg. 2009, 37: 405-11. |
| Zheng L., Ma L., Shi X. and Cheung L.K., Comparison of distraction osteogenesis versus costochondral graft in reconstruction of tempormandibular joint condylectomy with disc preservation, J Oral Maxillofac Surg . 2010, In press. |
| Zheng L., Ma L. and Cheung L.K., Comparison of gene expression of osteogenic factors between continuous and intermittent distraction osteogenesis in rabbit mandibular lengthening, Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology & Endodontics. 2009, 108 (4): 496-9. |
| Zheng L., Invited Reviewer, China Journal of Oral and Maxillofacial Surgery. 2009. |
| Zheng L., Invited Reviewer, International Journal of Oral Maxillofacial Surgery. 2009. |
| Zheng L., Invited Reviewer, Journal of Cranio-Maxillofacial Surgery. 2009. |
| Zheng L., Invited Reviewer, Journal of Oral Maxillofacial Surgery. 2009. |
| Zheng L., Invited Reviewer, Tissue Engineering. 2009. |
| Zheng L., Member of Editorial Board, Journal of Dentistry, Oral Medicine and Dental Education (Since 2008). 2009. |
| Zheng L., Member of Editorial Board, Journal of Medical and Biological Science (since 2008). 2009. |
| Zheng L., Molecular biology of distraction osteogenesis, Synthes Distraction Observership (Hong Kong). 2009. |
| Zheng L. and Cheung L.K., Stem cells in dentistry, Stem Cell and Regenerative Medicine by Herman S. Cheung (Ed.). Bentham Science Publishers Ltd, 2010, Chapter 10: 176-186. |
| Zheng L., Hua H. and Cheung L.K., Traditional Chinese medicine and oral diseases: today and tomorrow., Oral Dis . 2010, In press. |
| Zwahlen R.A., Cheung L.K., Zheng L., Chow R.L.K., Li T., Schuknecht B., Grätz K.W. and Weber F.E., Comparison of two resorbable membrane systems in bone regeneration after removal of wisdom teeth: a randomized-controlled clinical pilot study, Clinical Oral Implants Research. 2009, 20(10): 1084-91. |
| Researcher : Zhi Q |
| List of Research Outputs |
| Lo E.C.M., Zhi Q. and Itthagarun A., Comparing two quantitative methods for studying remineralization of artificial caries, Journal of Dentistry. 2010, 38: 352-359. |
| Researcher : Zhou W |
| Project Title: | Nanoclay Reinforced Tricalcium Phosphate Composite Coating to Enhance the Stability of Titanium Miniscrews |
| Investigator(s): | Zhou W, Rabie ABM, Wong RWK |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2009 |
| Abstract: |
| Objectives: The main objectives of the proposed project are to enhance the stability of orthodontic miniscrews and to increase their resistance to failure at higher ranges of force than currently used in the clinic. The specific objectives are: (1) To develop a “nanoclay/tricalcium phosphate (TCP) composite” that can help enhance the primary stability and partial osseointegration of miniscrews, (2) To impart nanofeatures to miniscrews through developing a novel method of “nanoemulsion” to coat the composite on the surface of miniscrews. (3) To demonstrate the in vitro biological compatibility of the newly developed composites, (4) To demonstrate the biomechanical properties of the composite coated miniscrew system. Problems being addressed and key issues: Miniscrews are small (1-2 mm in diameter) temporary anchorage devices (TADs) that are bone-born and provide absolute anchorage in orthodontics. They can withstand forces up to 300 grams and therefore are suitable for most orthodontic applications [1]. Even though TADs are gaining importance as an alternative source of intra-oral anchorage, their failure rates of 10–30% as described in the literature poses a clinical problem [2-6]. The factors presented below are currently regarded as possible reasons for the failure of TADs [7]. (1) Insufficient primary stability, (2) Application of excessive forces on the miniscrew, (3) A large lever arm (thick mucosa), (4) Peri-implantitis when inserted in the unattached mucosa, (5) Bone damage at insertion. Primary stability is the stability of the implant immediately after insertion (“Press-fit”). Bone quality, implant design, and insertion modalities are the fundamental factors affecting primary stability [7]. Osseointegration, the direct structural and functional connection between living bone and the surface of a load-bearing implant, could affect the long term stability of miniscrews. Recent studies showed that miniscrews used for temporary anchorage in orthodontics undergo partial osseointegration [8-11]. Primary Stability The proposed bionanocomposite of “nanoclay/TCP” is expected to enhance the primary stability as well as the degree of osseointegration by imparting nanofeatures to TADs. Nanoclay exhibits excellent mucoadhesive ability [12] and exceptionally high mechanical properties (Young’s modulus calculated at ca. 250-260 GPa [13]) that could positively enhance the primary stability of miniscrews. Partial Osseointegration Tricalcium phosphate (TCP), a biodegradable Ca-P, could dissolve in physiological media and be replaced by bone during implantation which will increase the degree of partial osseointegration of miniscrews [14]. Meanwhile the proposed nanofeatures could prolong the miniscrews’ retention time by adding nanoporosity as well as nanoroughness to the surface of the treated miniscrews. Nanoporosity and roughness have been shown to improve osseointegration through the development of enhanced coordinated functions of osteoclasts and osteoblasts [15-17]. The proposed nanoemulsion method will be developed and perfected to impart nanofeatures on titanium miniscrews. The TCP coating is biodegradable in physiological conditions. A feature that is critical to the easy removal of the mini-implant after use. The degree of biodegradability could be controlled by the level of crystallinity of the TCP. Crystallinity could be regulated by the developed nanoemulsion method where we will try to attain a level that could only biodegrade after a period of 5-6 months use. References: 1. Bae, S.M., H.S. Park, H.M. Kyung, O.W. Kwon, and J.H. Sung, Clinical application of micro-implant anchorage. Journal of Clinical Orthodontics, 2002. 36(5): p. 298-302. 2. Chen, Y.-J., H.-H. Chang, C.-Y. Huang, H.-C. Hung, E.H.-H. Lai, and C.-C.J. Yao, A retrospective analysis of the failure rate of three different orthodontic skeletal anchorage systems. Clinical Oral Implants Research, 2007. 18(6): p. 768-775. 3. Miyawaki, S., I. Koyama, M. Inoue, K. Mishima, T. Sugahara, and T. Takano-Yamamoto, Factors associated with the stability of titanium screws placed in the posterior region for orthodontic anchorage. American Journal of Orthodontics and Dentofacial Orthopedics, 2003. 124(4): p. 373-378. 4. Kravitz, N.D. and B. Kusnoto, Risks and complications of orthodontic miniscrews. American Journal of Orthodontics & Dentofacial Orthopedics, 2007. 131(4S): p. 43-51. 5. Kuroda, S., Y. Sugawara, T. Deguchi, H.M. Kyung, and T. Takano-Yamamoto, Clinical use of miniscrew implants as orthodontic anchorage: Success rates and postoperative discomfort. American Journal of Orthodontics & Dentofacial Orthopedics, 2007. 131(1): p. 9-15. 6. Kuroda, S., K. Yamada, T. Deguchi, T. Hashimoto, H.-M. Kyung, and T.T. Yamamoto, Root proximity is a major factor for screw failure in orthodontic anchorage. American Journal of Orthodontics and Dentofacial Orthopedics, 2007. 131(4, Supplement 1): p. S68-S73. 7. Wilmes, B., S. Ottenstreuer, Y.Y. Su, and D. Drescher, Impact of Implant Design on Primary Stability of Orthodontic Mini-implants. Journal of Orofacial Orthopedics, 2008. 69(1): p. 42-50. 8. Vande Vannet, B., M.M. Sabzevar, H. Wehrbein, and K. Asscherickx, Osseointegration of miniscrews: a histomorphometric evaluation. European Journal of Orthodontics, 2007. 29(5): p. 437-442. 9. Zubery, Y., N. Bichacho, O. Moses, and H. Tal, Immediate loading of modular transitional implants: a histologic and histomorphometric study in dogs. International Journal of Periodontics & Restorative Dentistry, 1999. 19(4): p. 343-353. 10. Nkenke, E., B. Lehner, K. Weinzierl, U. Thams, F. Neugebauer, H. Steveling, M. Radespiel-Troger, and F.W. Neukam, Bone contact, growth, and density around immediately loaded implants in the mandible of mini pigs. Clinical Oral Implants Research, 2003. 14(3): p. 312-321. 11. Costa, A., M. Raffainl, and B. Melsen, Miniscrews as orthodontic anchorage: a preliminary report. International Journal of Adult Orthodontics and Orthognathic Surgery, 1998. 13(3): p. 201-209. 12. Aguzzi, C., P. Cerezo, C. Viseras, and C. Caramella, Use of clays as drug delivery systems: Possibilities and limitations. Applied Clay Science, 2007. 36(1-3): p. 22-36. 13. Manevitch, O.L. and G.C. Rutledge, Elastic Properties of a Single Lamella of Montmorillonite by Molecular Dynamics Simulation. The Journal of Physical Chemistry B, 2004. 108(4): p. 1428-1435. 14. Best, S.M., A.E. Porter, E.S. Thian, and J. Huang, Bioceramics: Past, present and for the future. Journal of the European Ceramic Society, 2008. 28(7): p. 1319-1327. 15. Adamopoulos, O. and T. Papadopoulos, Nanostructured bioceramics for maxillofacial applications. Journal of Materials Science: Materials in Medicine, 2007. 18(8): p. 1587-1597. 16. Webster, T.J., C. Ergun, R.H. Doremus, R.W. Siegel, and R. Bizios, Enhanced functions of osteoblasts on nanophase ceramics. Biomaterials, 2000. 21(17): p. 1803-1810. 17. Webster, T.J., C. Ergun, R.H. Doremus, R.W. Siegel, and R. Bizios, Enhanced osteoclast-like cell functions on nanophase ceramics. Biomaterials, 2001. 22(11): p. 1327-1333. |
| Project Title: | Second International Conference on Polymer Processing and Characterization (ICPPC – 2010) Crystallization Kinetics of Apatite-Biopolymer Nanocomposites |
| Investigator(s): | Zhou W |
| Department: | Dental Faculty |
| Source(s) of Funding: | URC/CRCG - Conference Grants for Teaching Staff |
| Start Date: | 01/2010 |
| Completion Date: | 01/2010 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Zhou W., Crystallization Kinetics of Apatite-Biopolymer Nanocomposites, Second International Conference on Polymer Processing and Characterization (ICPPC). 2010, IL112. |
| Zhou W., Crystallization Kinetics of Apatite-Biopolymer Nanocomposites, Second International Conference on Polymer Processing and Characterization (ICPPC). Kerala, India, 2010, IL112. |
| Zhou W., Rabie A.B.M., Wong R.W.K. and Tang B., Nanocoating of Montmorillonite/Mg-β-Tricalcium Phosphate on Orthodontic Titanium Miniscrews, 3rd IEEE International NanoElectronics Conference (INEC). 2010, BC211. |
| Zhou W., Guo B., Liu M., Liao R. and Jia D., Poly(vinyl alcohol)/Halloysite Nanotubes Bionanocomposite Films: Properties and In Vitro Osteoblasts and Fibroblasts Response, Journal of Biomedical Materials Research: Part A. 2009, 93A: 1574-1587. |
| Zhou W., Wang M., Cheung W.L. and Ip W.Y., Selective Laser Sintering of Poly(L-Lactide)/Carbonated Hydroxyapatite Nanocomposite Porous Scaffolds for Bone Tissue Engineering, In: Daniel Eberli , Tissue Engineering. Vienna, Austria, IN-TECH, 2010, 179-204. |
| Researcher : Zhu H |
| List of Research Outputs |
| Zhu H., McMillan A.S. and McGrath C.P.J., Oral health-related quality of life after stroke, Journal of Dental Research. 2009, 88 (Spec Iss B): 610 (PAPF/APR). |
| Researcher : Zhu S |
| List of Research Outputs |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia . 2010, March/April 2010: 21-27. |
| Ng A.F.S., Zhu S., Wong R.W.K., Hagg E.U.O. and Wiechmann D., Indirect orthodontic bracket bonding technique and a review of the literature, Dental Asia November/December 2009. 2009, 24-29. |
| Zhu S., Yang Y. and Wong R.W.K., Poster Presentation: A Comparative Study on the Alignment Efficiency of a Lingual System, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June. 2010. |
| Researcher : Zuo P |
| Project Title: | Codon optimization for cellulase |
| Investigator(s): | Zuo P, Rabie ABM |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2009 |
| Completion Date: | 11/2010 |
| Abstract: |
| Cellulose is the most common organic compound on Earth. About 33 percent of all plant matter is cellulose (the cellulose content of cotton is 90 percent and that of wood is 50 percent). It is an organic compound with the formula (C6H10O5)n, a polysaccharide consisting of a linear chain of several hundred to over ten thousand β(1→4) linked D-glucose units (Updegraff 1969). The difference between cellulose and starch is that the starch is a polysaccharide of linear chain of α- linked glucose. Starch is a major food source for humans. However, the cellulose can not be a food source for humans because there is not any enzyme broken β(1→4) linked bonds in human bodies. Cellulase catalyze the cellulolysis (or hydrolysis) of cellulose. It can break the β(1→4) linked D-glucose units into glucose. Once it treat the cellulose into glucose, the glucose can be easily used by food, energy or other industrial material. To get better and more cellulase, we propose to optimize the condon of cellulase. |
| List of Research Outputs |
| Almoqubel A., Wong R.W.K., Zuo P. and Rabie A.B.M., Poster Presentations: Stem Cell Differentiation into Angiogenesis Lineage, The 86th Congress of the European Orthodontic Society in Portoroz, Slovenia, 15 – 19 June, 2010. |
| Zuo P. and Rabie A.B.M., Novel method of cell-free in vitro synthesis of the human fibroblast growth factor 1 gene, Journal of Biomedicine and Biotechnology. 2010. |
| Researcher : Zwahlen RA |
| Project Title: | Release of vessel development in heterotopically cultured bone |
| Investigator(s): | Zwahlen RA, Cheung LK, Zheng L |
| Department: | Dental Faculty |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 03/2008 |
| Completion Date: | 02/2010 |
| Abstract: |
| Key issues and problems being addressed: 1. Due to radical surgical resections of benign or malignant tumours in the field of cranio-maxillofacial and oral surgery often large bony defects have to be created. Partial or total agenesis of bone may occur in children suffering from cranio-maxillofacial dysplasia syndromes. Both types of defects usually are reconstructed using extensive autologous bone grafts from the ribs, fibula, iliac crest or cranial vault in order to reestablish the function and future dental rehabilitation. Both the size of the bone transplant and the additional lengthening of the operation time for autologous bone harvest increase the donor-site morbidity. 2. The critical size bone defect of about 5cm represents a non to underestimate obstacle in reconstruction. From this size on, only microvascular reanastomised bone grafts are considered to be still reliable, due to their predictable proper blood circulation. Free autologous bone grafts of this size often become centrally necrotic yet before blood vessels from the local bone margins sprout. 3. For tissue engineering purposes, in vitro bioreactors have been designed to mimic natural environment. The artificial in vitro bioreactors only allow the growth of small pieces of tissue. Vascularization and nutrition remains a problem, limiting the size of these constructs. 4. Fabrication of a heterotopical bone graft by in vivo tissue engineering in a region with suitable conditions such as muscle for bone induction has demonstrated its potential in reconstructing critical size bone defect. However, for aged or malignant cancer patients after irradiation, the survival of such a prefabricated graft remains a concern within the compromised environment. 5. Efforts of using recombinant proteins such as recombinant human BMP-2 and VEGF have been reported for years. One of the major problems in clinical application poses the fast flush away is of the administered proteins within the body. Thus high dosages of growth factors are usually required to achieve satisfying results. High dosages of these proteins, however, raise concerns regarding the safety as well as costs. Main study hypothesis: Heterotopic bone formation can be accomplished using rhBMP-2 embedded in fibrin as carrier matrix, and the addition of TG-VEGF to the compound of fibrin and rhBMP-2 may lead to vessel sprouting out of the anastomosis of the femoral artery and vein within the INION-mesh-tube. Study objectives: 1. Study the feasibility of fabricating heterotopic bone by interaction of fibrin/rhBMP-2 and muscle tissue in a rabbit model. 2. Assess the effect of TG-VEGF on enhancing vessel sprouting out of the anastomosis within the fabricated bone graft. 3. Evaluate the synergistic effect between rhBMP-2 and TG-VEGF at different ratios on enhancing angiogenesis and bone formation. |
| Project Title: | Quality of life, psychosocial adjustment and postraumatic growth of patients with oral cavity cancer and their spouses: a prospective multicenter study |
| Investigator(s): | Zwahlen RA |
| Department: | Dental Faculty |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2010 |
| Abstract: |
| Aim of the study: To prospectively examine quality of life, emotional distress, psychosocial adjustment and psychological growth among patients treated for oral cavity cancer (OCC) and their partners and to determine psychosocial dysfunction, the onset of eventual psychiatric morbidity in patients and partners and the need for psychosocial support in a multicentre study; secondly, to determine associations of patients and their partners quality of life and psychosocial distress and ethnic differences. Even though patients with tumours of the oral cavity suffer from a life-threatening disease, mostly combined with functional limitations and aesthetic disfigurement due to surgical treatment, their quality of life (QoL) compared to healthy population is good and their psychosocial burden is low (Vickery et al.,2003; Zwahlen et al.,2008). The disease of a malignant tumour often leads to a compromised QoL of the spouse, being combined with psychological distress (Pitceathly et al., 2003; Drabe et al, 2008). In a retrospective cross-sectional study Drabe et al, 2008 found a very high percentage of agoraphobia (23%) in examined female spouses, representing a multiple of the normal distribution of this disease among normal population (7%). Further on patients with oral cavity tumours are severely compromised in the social field (speech, eating, aesthetic etc.), what probably might lead additionally to enhanced psychosocial burden of the spouses. On the other hand it is known that spouses and family members are key players in the support of cancer patients and that the quality of that support is linked to the QoL and the psychological distress of the patient (M.R. Katz et al., 2003). The aim of this prospective multi-centre study is to examine the QoL and the psychosocial distress of patients with oral cancer and their spouses as well as to determine their need of psychosocial support. Further on it shall be investigated how QoL and psychological distress of oral cancer patients are modified by the spouses. The multi-centre design was chosen, to find out, whether there are ethnic differences among the parameters as well as to get an appropriate number of patients. If, as supposed, the patients’ QoL correlates with the one of the spouses and psychiatric diseases of the spouses would be connected with the diagnosis of their partners, this would result in major implications related to the treatment plan and to the further support of oral cancer patients and their spouses. Research questions: The following main research questions will be answered: • Do psychiatric diseases onset in patients and spouses at the diagnosis of cancer? • Do male spouses show the same psychological discomfort as the female spouses? • Do female patients suffer from more severe limitation of QoL, psychological distress than male patients? • Do female patients feel the same support from their male spouses as vice versa? • Are there ethnical differences related to QoL, psychiatric morbidity, psychological growth and / or dyadic system? |
| Project Title: | Release of vessel development in heterotopically cultured bone |
| Investigator(s): | Zwahlen RA, Cheung LK, Zheng L |
| Department: | Dental Faculty |
| Source(s) of Funding: | General Research Fund (GRF) |
| Start Date: | 01/2010 |
| Abstract: |
| 1) Study the possibility of neo-vessels sprouting out of the anastomosis of the femoral artery and vein; 2) Assess the heterotopical bone formation in the INION-mesh-tube filled with fibrin scaffold carrying rhBMP-2 around the anastomosis; 3) Assess the effect of TG-VEGF on enhancing vessel sprouting out of the anastomosis within the fabricated bone graft. |
| List of Research Outputs |
| Bütow K.W., Hoogendijk C.F. and Zwahlen R.A., Pierre Robin sequence: appearances and 25 years of experience with an innovative treatment protocol, Journal of Pediatric Surgery. 2009, 44 (11): 2112-8. |
| Jayaratne Y.S.N., Lo J., Zwahlen R.A. and Cheung L.K., 3-D Photogrammetry for Surgical Planning of Tissue Expansion in Hemifacial Microsomia., Head Neck. PMID 19862829, 2009. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Computer Aided Maxillofacial Surgery: An Update (accepted), Surgical Innovations. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A. and Cheung L.K., Three-dimensional anthropometric analysis of the Chinese nose, J Plast Reconstr Aesthet Surg. 2010. |
| Jayaratne Y.S.N., Zwahlen R.A., Lo J. and Cheung L.K., Three-dimensional color maps: a novel tool for assessing craniofacial changes., Surg Innov. 2010. |
| Kruse A.L., Zwahlen R.A., Bredell M.G., Riener M.O. and Graetz K.W., Apocrine hidrocystoma of the cheek, J Craniofac Surg. 2010, 21(2): 594-596. |
| Kruse A.L.D., Zwahlen R.A. and Grätz K.W., New classification of maxillary ameloblastic carcinoma based on an evidence-based literature review over the last 60 years, Head & Neck Oncology. 2009, 1 (31): 1-7. |
| Kruse A.L.D., Zwahlen R.A., Bredell M.G., Gengler C., Dannemann C. and Graetz K.W., Primary Blastomycosis of Oral Cavity, J Craniofac Surgery. 2010, 21: 121-123. |
| Lo J., Xia J.J., Zwahlen R.A. and Cheung L.K., Surgical navigation in correction of hemimandibular hyperplasia: a new treatment strategy, J Oral Maxillofac Surg. 2010, 68: 1444-50. |
| Sun Z.J., Cai Y., Zwahlen R.A., Zheng Y.F., Wang S.P. and Zhao Y.F., Central giant cell granuloma of the jaws: clinical and radiological evaluation of 22 cases, Skeletal Radiology. 2009, 38(9): 903-9. |
| Wu Z.X., Zheng L., Li Z.B., Yang X.W., Dong Y.J., Zhao Y.F. and Zwahlen R.A., Subapical anterior maxillary segmental osteotomy: a modified surgical approach to treat maxillary protrusion, J Craniofac Surg. 2010, 21: 97-100. |
| Zwahlen R.A., ATLS Advanced Trauma Life Support, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Access surgery to the facial skeleton, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Airway control - conio and tracheostomy, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Assessment of postoperative outcome: 3D photogrammetry, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Biomechanical behaviour/strength of the mandibular neck, AO CMF Asia (Association of internal fixation), AO-CMF Principal Course, 10th to 17th Sept, Ho Chi Minh City, Vietnam. 2009. |
| Zwahlen R.A., Classification of facial fractures, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Cheung L.K., Zheng L., Chow R.L.K., Li T., Schuknecht B., Grätz K.W. and Weber F.E., Comparison of two resorbable membrane systems in bone regeneration after removal of wisdom teeth: a randomized-controlled clinical pilot study, Clinical Oral Implants Research. 2009, 20(10): 1084-91. |
| Zwahlen R.A., Defect reconstruction - daybreak of oral rehabilitation, Association of Oral & Maxillofacial Surgeons - Singapore Scientific Symposium 7th December 2009, Singapore. 2009. |
| Zwahlen R.A., Dental trauma including dentoalveolar fractures, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Facial bite wounds, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Guest Professor (2009 - present), Wuhan University, School of Stomatology, Wuhan, PR-China. 2009. |
| Zwahlen R.A., Honorary Consultant (2008 - February 2010), Department of Oral and Maxillofacial Surgery, Shenzhen Hospital, Beijing University, Shenzhen, PR-China. 2009. |
| Zwahlen R.A., Honorary Professor (Since 2010 February), Department of Oral and Maxillofacial Surgery, Shenzhen Hospital, Beijing University, Shenzhen, PR China. 2010. |
| Zwahlen R.A., How to fix the occlusion: Ernst ligaturesm, arch bar, IMF screw. Dental & dentoalveolar trauma, AO CMF Asia (Association of internal fixation), AO-CMF Principal Course,10th to 17th Sept, Ho Chi Minh City, Vietnam. 2009. |
| Zwahlen R.A., Interdisciplinary management of severe midfacial fractures, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Mandibular fractures , IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Midfacial and Frontal Sinus fractures, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Orbital fractures and ocular trauma, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Organizer, Conjoint Dental Scientific Meeting, The University of Hong Kong and Peking University, 16-17 November 2009. 2009. |
| Zwahlen R.A., Osteotomy of the mandible and fixation, AO CMF Asia (Association of internal fixation), AO-CMF Principal Course, 10th to 17th Sept, Ho Chi Minh City, Vietnam. 2009. |
| Zwahlen R.A., Pediatric facial fractures, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Privatdozent (from 2009 - present), Department of Cranio-Maxillofacial and Oral Surgery, University Hospital Zurich, Zurich, Switzerland. 2009. |
| Zwahlen R.A., Soft tissue treatment and treatment after massive tissue loss, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Surgical Approaches to the Midface, AO CMF Asia (Association of internal fixation), AO-CMF Principal Course, 10th to 17th Sept, Ho Chi Minh City, Vietnam. 2009. |
| Zwahlen R.A., Surgical navigation, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Traumatic brain injury, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Treatment of the elderly trauma patient, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Visiting Professor (2009 - 2012), Department of Head and Neck Surgery, Sun Yat-sen Cancer Centre, University of Guangzhou, Guangzhou, PR China. 2009. |
| Zwahlen R.A., Who's Who in the World; Biographical Record , Who's Who Marquis Who's Who Publications Board. Twenty-Seventh Edition 2010. Editor in Chief: Marks FM. 2010. |
| Zwahlen R.A., Zgomatic fractures - techniques and approaches, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |
| Zwahlen R.A., Zygoma and zygomatic arch fractures, IAOMS - Trauma Course - 21st to 24th April 2010, Hospital Sungai Buloh, Kuala Lumpur, Malaysia. 2010. |