| THE
UNIVERSITY OF HONG KONG |
DEPARTMENT OF BIOCHEMISTRY
| Researcher : Chan CH |
| List of Research Outputs |
| Chan C.H., Shum D.K.Y. and Ip M.S.M., Sputum sol neutrophil elastase activity in bronchiectasis: differential modulation by syndecan-1 , American Journal of Respiratory and Critical Care Medicine. 2003, 168(2): 192-198. |
| Researcher : Chan D |
| Project Title: | Defining molecular mechanisms underlying growth plate abnormalities in Schmid metaphyseal chondrodysplasia by targeted mutagenesis in mice |
| Investigator(s): | Dr. Chan D., Prof. Cheah K.S.E., Dr. Cheung K.M.C. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 11/2000 |
| Abstract: |
| Schmid metaphyseal chondrodysplasia (SMCD) is an autosomal dominant skeletal disorder caused by defects in the growth plate. Collagen X is the most abundant extracellular matrix component synthesized specifically by hypertrophic chondrocytes within the growth plate. Mutations in the collagen X gene (COL10AI) result in SMCD. The project aims to generate the equivalent of two known human COL10AI SMCD mutations in mice for observation. |
| Project Title: | Molecular and cellular changes underlying increased bone formation in a mouse model with generalized progressive hyperostosis |
| Investigator(s): | Dr. Chan D., Prof. Cheah K.S.E. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 10/2001 |
| Abstract: |
| To determine the fate of Co110-13del protein by analysing its synthesis, secretion and degradation in primary osteoblast cultures, and correlate the finding with possible contributing factors; to test primary osteoblasts for constitutive activation and the capacity to promote active bone formation by monitoring collagen synthesis, in vitro matrix formation/composition, mineralisation, and matrix architecture surrounding cells; to determine whether osteoclast activity and/or osteoclastogenesis contribute to the increase-bone phenotype. |
| Project Title: | A matrix metalloproteinase detector for in vitro monitoring of enzyme activity |
| Investigator(s): | Dr. Chan D., Prof. Lam E. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 06/2002 |
| Abstract: |
| To establish efficient in vitro reporter assays for aggrecanase and other MMPs. |
| Project Title: | The role of matrix remodeling in endochondral bone formation |
| Investigator(s): | Dr. Chan D., Prof. Cheah K.S.E. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 10/2002 |
| Abstract: |
| The project attempts to:- 1) create heterozygous and homzygous mouse mutants expressing MMP-resistant collagen X from the four embryonic stem (ES) cell clones; 2) perform detailed in virto and in vivo analysis to study the molecular and phenotypic consequences on endochondral bone formation. |
| Project Title: | Understanding the molecular basis of skeletal (digit) patterning defects in Brachydactylyl type A-1 |
| Investigator(s): | Dr. Chan D., Prof. Cheah K.S.E., Prof. He L. |
| Department: | Biochemistry |
| Source(s) of Funding: | National Natural Science Foundation of China |
| Start Date: | 01/2003 |
| Abstract: |
| To create a BDA-1 mouse model by gene targeting; to study the impact of the BDA-1 mutation on skeletal morphogenesis; to study the biochemical consequence of BDA-1 mutations using in vitro cell approaches. |
| Project Title: | Defining the molecular basis and 'factors' promoting osteoblast activity in a mouse model with generalized hyperostosis |
| Investigator(s): | Dr. Chan D., Prof. Cheah K.S.E., Dr. Cheung K.M.C., Dr. Chu I.K. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 07/2003 |
| Abstract: |
| To define the molecular basis and "factors" promoting osteoblast activity in a mouse model with generalized progressive hyperostosis. |
| List of Research Outputs |
| Abbah S.A., Lu W.W., Liu W., Chan D., Cheung K.M.C., Leong J.C.Y. and Luk K.D.K., Osteogenic differentiation of alginate microencapsulated mesenchymal stem cells for bone formation: an in vitro study, 7th World Biomaterials Congress, Sydney, Australia, May 17-22, 2004. |
| Chan D., Ho M.S.P., Tsang K.Y., Chan W.Y., Sillence D., Boot-Handford R., Cheung K.M.C. and Cheah K.S.E., Abnormal chondrocyte differentiation is dosage-dependent in transgenic mice expressing unassembled mutant collagen X in hypertrophic chondrocytes, Human Genome Meeting, Berlin, Germany, April 2004. |
| Chan W.Y., Cheah K.S.E., Murphy G. and Chan D., Role of matrix remodeling in endochondral bone formation, 5th Pan-Pacific Connective Tissue Society Symposium, Ube City, Yamaguchi, Japan, 3-7 June 2003. |
| Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., Kwan K.M., Cheung K.M.C., Hunziker E., Bateman J.B. and Chan D., In vivo plasticity in terminal differentiation of hypertrophic chondrocytes induced by ER stress. , Mouse Molecular Genetics Meeting, EMBL, Heidelberg, Germany, September 2003. |
| Cheung K.M.C., Jim J.J.T., Noponen-Hietala N., Ott J., Karppinen J., Luk K.D.K., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Age dependent association of the COL9A2 Trp2 allele with intervertebral disc degeneration, annular tears and end-plate herniations, 38th Annual Meeting of the Scoliosis Research Society, Quebec, Canada, September 10-13, 2003. |
| Cheung K.M.C., Chan W.C.W., Cheah K.S.E. and Chan D., The molecular and cellular changes underlying increased bone formation in a mouse model with generalized progressive hyperostosis, European Cells and Materials. 2003. |
| Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Song Y.Q., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Genetic variation in the COL9A2 gene and its association to intervertebral disc abnormalities in Southern Chinese, Hong Kong Orthopaedic Association, Hong Kong, November 8-9, 2003. |
| Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Luk K.D.K., Yip S.P., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Genetic variation in the Col9A2 gene and its association to intervertebral disc abnormalities in Southern Chinese, XIX International Congress of Genetics, Melbourne, Australia, July 2003. |
| Law S.K.F., Cheah K.S.E., He L., Gao B. and Chan D., The molecular basis of Indian hedgehog mutations in digit patterning, Human Genome Meeting, Berlin, Germany, April 2004. |
| Leung W.L., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) Signalling, 8th Postgraduate Symposium, HKU, December 2003 Hong Kong . 2003. |
| Leung W.L., Wong S.Y.Y., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) signaling., 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June 2004 Hong Kong.. 2004. |
| Lu W.W., Abbah S.A., Luk K.D.K., Cheung K.M.C., Liu W., Chan D., Li Z. and Leong J.C.Y., Osteogenic differentiation of alginate microencapsulated mesenchymal stem cells for bone formation in osteoporosis spine, SPINEWEEK 2004, Porto, Portugal, May 30-June 5, 2004. |
| Song Y., Chan D., Karppinen J., Chen Y., Leong J.C.Y., Luk K.D.K., Cheah K.S.E. and Cheung K.M.C., Study of Polymorphisms of Vitamin D Receptor gene in Lumbar Disc Degeneration in Chinese, Human Genome Meeting, Berlin, Germany, April 2004,. 2004, Abstract No. 118. |
| Researcher : Chan KFJ |
| Project Title: | Signal transduction in the brain: roles of ganglioside-stimulated protein kinase |
| Investigator(s): | Dr. Chan K.F.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/1993 |
| Abstract: |
| To establish the functional roles for a novel ganglioside-stimulated protein kinase. These investigations are designed to provide further evidence for our unifying hypothesis that gangliosides can serve as multifuncitonal biomodulators in neural signal transduction. |
| Project Title: | Signal transduction in the brain: roles of ganglioside-stimulated protein kinase |
| Investigator(s): | Dr. Chan K.F.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | Wing Lung Bank Medical Research Fund |
| Start Date: | 07/1993 |
| Abstract: |
| To establish the functional roles for a novel ganglioside-stimulated protein kinase. These investigations are designed to provide further evidence for our unifying hypothesis that gangliosides can serve as multifuncitonal biomodulators in neural signal transduction. |
| Project Title: | Molecular cloning and expression of Schistosome fucosyltransferase for vaccine development |
| Investigator(s): | Dr. Chan K.F.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/1995 |
| Abstract: |
| To characterize the a1-3 fucosyltransferase found in Schistosoma japonica through molecular cloning, DNA sequencing and protein expression of the parasitic enzyme. These investigations are prerequisites in the development of a vaccine for prevention of Schistosome infection and the spread of the disease. |
| Project Title: | Regulation of nerve cell synaptic vesicle protein phosphorylation by ganglioside-stimulated protein kinase and traditional Chinese medicinal herbs |
| Investigator(s): | Dr. Chan K.F.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/1996 |
| Abstract: |
| To provide evidence for a functional role of ganglioside-stimulated protein kinase in the regulation of neurotransmitter release. The bioassay systems developed during this investigation are applicable for subsequent determination and identification of neuroactive components in traditional Chinese medicinal herbs. |
| Researcher : Chan KK |
| List of Research Outputs |
| Chan K.K., Chen A.Y.S., Lui V.C.H., Tam P.K.H. and Sham M.H., Abnormal enteric ganglia development in transgenic mice with ectopic expression of mutant Sox10 in the vagal neural crest. , The 4th International Meeting on Hirschprung Disease and Related Neurocristopathies HSCR, April 2004, Genova italy. 2004. |
| Chan K.K., Wong C.K.Y., Lui V.C.H., Tam P.K.H. and Sham M.H., Analysis of SOX10 mutations identified in Waardenburg-Hirschsprung patients: differential effects on target gene regulation, Journal of Cellular Biochemistry. 2003, 90(3): 573-585. |
| Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interactions of Hox genes during craniofacial development. , 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Researcher : Chan LC |
| List of Research Outputs |
| Lam V.M.S., Au D.M.Y. and Chan L.C., Multiplex detection: a rapid and reliable method to screen common variations in the Glucose-6-phosphate dehydrogenase gene. , Mutation Detection Workshop, satellite meeting of the International Congress of Genetics, July, Australia.. 2003. |
| Researcher : Chan WCW |
| List of Research Outputs |
| Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., Kwan K.M., Cheung K.M.C., Hunziker E., Bateman J.B. and Chan D., In vivo plasticity in terminal differentiation of hypertrophic chondrocytes induced by ER stress. , Mouse Molecular Genetics Meeting, EMBL, Heidelberg, Germany, September 2003. |
| Cheung K.M.C., Chan W.C.W., Cheah K.S.E. and Chan D., The molecular and cellular changes underlying increased bone formation in a mouse model with generalized progressive hyperostosis, European Cells and Materials. 2003. |
| Researcher : Chan WY |
| List of Research Outputs |
| Chan D., Ho M.S.P., Tsang K.Y., Chan W.Y., Sillence D., Boot-Handford R., Cheung K.M.C. and Cheah K.S.E., Abnormal chondrocyte differentiation is dosage-dependent in transgenic mice expressing unassembled mutant collagen X in hypertrophic chondrocytes, Human Genome Meeting, Berlin, Germany, April 2004. |
| Chan W.Y., Cheah K.S.E., Murphy G. and Chan D., Role of matrix remodeling in endochondral bone formation, 5th Pan-Pacific Connective Tissue Society Symposium, Ube City, Yamaguchi, Japan, 3-7 June 2003. |
| Researcher : Chau CH |
| List of Research Outputs |
| Chau C.H., Shum D.K.Y., Li H., Pei J., Lui Y.Y., Wirthlin L., Chan Y.S. and Xu X.M., Chondronitase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury, FASEB Journal. 2004, 18(1): 194-196. |
| Researcher : Cheah KSE |
| Project Title: | Type XI collagen gene expression in mouse and man |
| Investigator(s): | Prof. Cheah K.S.E., Prof. Grant M.E. |
| Department: | Biochemistry |
| Source(s) of Funding: | The Arthritis and Rheumatism Council, UK |
| Start Date: | 01/1989 |
| Abstract: |
| The production of DNA and monoclonal antibody probes for the detection of expression of type XI collagen mRNA and protein. These tools will be used to study the expression of the alpha chains of type XI collagen during embryogenesis in mouse and man. These studies should extend our knowledge of the structure, function, growth and development of cartilage and bone. |
| Project Title: | Defining type X collagen function; transgenic mouse and gene targeting approaches |
| Investigator(s): | Prof. Cheah K.S.E. |
| Department: | Biochemistry |
| Source(s) of Funding: | The Arthritis and Rheumatism Council, UK |
| Start Date: | 02/1992 |
| Abstract: |
| To use gene targeting in mouse embryonal stem cells and mouse chimaeras to determine and study the function of type X collagen; to study early onset osteoarthritis in STR/ORT mice as a model for the human disease. |
| Project Title: | The role of Type II procollagens encoded by alternatively spliced forms of aI(II) procollagen mRNA in cartilage differnetiation and growth |
| Investigator(s): | Prof. Cheah K.S.E. |
| Department: | Biochemistry |
| Source(s) of Funding: | The Arthritis and Rheumatism Council, UK |
| Start Date: | 01/1994 |
| Abstract: |
| To gain insight into the function of type IIA procollagen by: a) performing a loss-of function test by introducing into the mouse germ line, a mutation in the a1(II) collagen gene in which exon 2 is deleted, using gene targeting; determining the phenoypic consequence of reduced levels of expression or failure to express type IIA procollage; generating monoclonal antibodies to the cysteine-rich domain within the aminopropeptide of type IIA procollagen. |
| Project Title: | Defining the role of the transcription factor Sox9 in skeletal development by tissue-specific gene inactivation |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Lovell-Badge R.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | UK/HK Joint Research Scheme (British Council / Hong Kong Research Grants Council) |
| Start Date: | 02/1998 |
| Abstract: |
| To inactivate the mouse Sox9 gene selectively and specifically in developing cartilage tissue in order to understand the role of Sox9 in skeletal formation and the relationship between loss of Sox9 gene function and skeletal malformation. |
| Project Title: | Understanding gene function and molecular bases of disease using transgenic and gene targeting technology |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chan W.Y., Dr. Chan D., Prof. Chan L.C., Dr. Chan S.Y., Dr. Chung S.K., Dr. Han Y.F., Dr. O W.S., Dr. Sham M.H., Dr. Tam P.P.L., Prof. Wang J.H.C., Prof. Wong Y.C., Prof. Xue H.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Central Allocation Vote |
| Start Date: | 03/1999 |
| Abstract: |
| To use gene targeting and "Cre-lox" technology in a concerted research program to unravel the functional roles of several genes in disorders affecting cell proliferation and differentiation during: a) formation of the growth plate and craniofacial skeleton, b) neuronal differentiation, c) haematopoietic differentiation. |
| Project Title: | Molecular and developmental characterisation of inner ear abnormalities in the novel transgenic mouse mutant, yellow submarine (ysb) |
| Investigator(s): | Prof. Cheah K.S.E., Prof. Chan Y.S., Prof. Steel K. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/1999 |
| Abstract: |
| To identify and characterise the gene(s) at the wild-type ysb(+ysb) locus; to determine the nature of the ysb mutation; to study the molecular and developmental bases underlying the defect(s) in ysb mice; to characterise the neurophysiological changes in balance and hearing in ysb mice. |
| Project Title: | Feasibility study on the development of analytical methodology to establish the authenticity of bird nests |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Wong N.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Miscellaneous external fund sources |
| Start Date: | 06/2000 |
| Abstract: |
| To carry out a feasibility study on the development of analytical methodology to establish the authenticity of bird nests. |
| Project Title: | Functional analysis of the morphogenetic role of type IIA procollagen during mouse development by conditional tissue-specific gene inactivation |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chan D., Dr. Tam P.P.L. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 01/2001 |
| Abstract: |
| To use tissue-specific gene inactivation in mice, molecular embryology and biochemistry to test the hypothesis that IIA procollagen has a morphogenetic role in tissue patterning during organogenesis. |
| Project Title: | Molecular genetic and biochemical analyses of hereditary bone tumours (osteochondromas / exostosis) in Chinese |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chan D., Prof. Chen S.M., Dr. Cheung K.M.C., Dr. Guan X.Y., Dr. Huang J.D., Dr. Luo Z.J., Dr. Shum D.K.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | National Natural Science Foundation of China |
| Start Date: | 01/2001 |
| Abstract: |
| To make a clinical characterization of the affected members; to identify the mutant locus; to isolate the mutant gene and determine the nature of the mutation; to study the impact of the mutation on cartilage and bone differentiaiton and growth; to establish cell cultures from the exostoses and study proteoglycan biosynthesis in the mutant cells. |
| Project Title: | In vivo functional analysis of the SOX9 transcription factor in regulating developmental gene expression |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chan D., Dr. Tam P.P.L. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2001 |
| Abstract: |
| To use transactivation of the COL2A1 enhancer reporter upon ectopic expression of SOX in transgenic mice to assess: i) the functional requirement for SOX9 to activate its target genes by testing the competence of SOX2 and SOX8 to substitute for SOX9; ii) the requirements for the N-, HMG, C terminus regions of SOX9 in activating its target genes by testing the competence of SOX9-SOX2 and SOX9-SOX8 chimeric proteins to substitute for SOX9 by gene targeting into the Sox9 locus in mouse ES cells, to test further the competence of the alternative SOX gene and/or relevant chimeric SOX protein to mediate the differentiation and developmental roles of SOX9, with particular focus on chondrogenesis. |
| Project Title: | Genomic approaches to uncover functionally relevant signalling pathways in craniofacial development |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chow K.L., Dr. Chung S.K., Dr. Huang J.D., Dr. Sham M.H., Dr. Smith D.K., Dr. Tam P.P.L., Dr. Wicking C.A., Dr. Wong B.C.W., Dr. Yang M.M.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Central Allocation Vote |
| Start Date: | 02/2002 |
| Abstract: |
| To build a multidisciplinary, competitive and productive research team of a critical size with the central theme of uncovering genetic relationships and functionally relevant signalling pathways in craniofacial development in order to make an impact in this important research area, currently at the fore-front of genomic biology; to pool expertise and resources, employing complementary genetic approaches in mice, coupled with advanced technology to reveal networks of genes and the complex interactions that specify morphology of the head skeleton and facial structures; to use and develop bioinformatics tools to analyse the expression data and formulate hypotheses on genetic relationships and the pathways regulating patterning, formation and growth of the craniofacial primordia; to test the hypotheses on potential functional relationships and molecular interactions revealed by the bioinformatic analyses, in the worm model and in the yeast two hybrid system. |
| Project Title: | The regulation and role of Sox2 in the circling, deaf and yellow mouse mutants Yellow submarine (Ysb) and Light coat and circling (Lcc) |
| Investigator(s): | Prof. Cheah K.S.E. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 10/2002 |
| Abstract: |
| The current project will test some hypotheses of Sox2 function using gene mapping, comparative genomics, genetics and transgenesis to: - i) identify the chromosomal breakpoints in Lcc; ii) identify the mutations in Ysb and Lcc and determine whether these have disrupted cis-elements which regulate Sox2 expression in the inner ear and hair follicle; iii) validate these findings by functional rescue/mutagenesis/genetic experiments in transgenic mice; iv) identify the molecular developmental changes in inner ear hair cell and hair follicle morphogenesis. |
| Project Title: | Understanding gene function and molecular bases of disease using transgenic and gene targeting technology |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chan W.Y., Dr. Chan D., Dr. Chan S.Y., Prof. Chan L.C., Dr. Chow K.L., Dr. Chow B.K.C., Dr. Chung S.K., Dr. Huang J.D., Prof. Kung H.F., Dr. Lin M.C.M., Dr. Lung M.L., Dr. Sham M.H., Dr. Tam P.P.L., Prof. Waye M.M.Y., Dr. Zhang J.C.J.L. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Central Allocation Vote |
| Start Date: | 06/2003 |
| Abstract: |
| To continue to build up a multidisciplinary, competitive and productive research team of a critical size pooling expertise and resources to tackle several fore-front issues of human diseases using animal models; to maintain a highly productive Transgenic Core Facility (TCF) with extended "clientele", facilitating the timely generation of genetically modified mice for more projects which will serve to provide new knowledge in understanding gene function and as models of human disease, provide insight into the molecular pathogenesis of disease. |
| Project Title: | Genetic manipulation and analyses of the regulation of chondrocyte hypertrophy |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chan D. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2003 |
| Abstract: |
| To understand the roles of Ihh and Ppr specifically in hypertrophic chondrocytes (HCs) and will dissect the mode of action of the Ihh and PPR pathways in HCs, in vivo, in transgenic and conditional knockout mice, Specially we will: 1) Investigate whether abnormal Ihh signaling and /or processing in 13del HCs is a primary cause of the differentiation abnormality by a) ablating Smoothened (Smo), the transducer of Ihh signaling in 13 del and wild-type HCs and b) over-expressing either Ihh or a non-secreted and unprocessed form of Ihh in normal HCs. 2) By the same rationale, study the role of the PTHrP/PPR pathway by ablating PPR in 13 del and wild-type HCs. |
| Project Title: | Genetic manipulation and analyses of the regulation of chondrocyte hypertrophy |
| Investigator(s): | Prof. Cheah K.S.E., Dr. Chan D. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 09/2003 |
| Abstract: |
| To understand the roles of Ihh and Ppr specifically in hypertrophic chondrocytes (HCs) and will dissect the mode of action of the Ihh and PPR pathways in HCs, in vivo, in transgenic and conditional knockout mice, Specially we will: 1) Investigate whether abnormal Ihh signaling and /or processing in 13del HCs is a primary cause of the differentiation abnormality by a) ablating Smoothened (Smo), the transducer of Ihh signaling in 13 del and wild-type HCs and b) over-expressing either Ihh or a non-secreted and unprocessed form of Ihh in normal HCs. 2) By the same rationale, study the role of the PTHrP/PPR pathway by ablating PPR in 13 del and wild-type HCs. |
| List of Research Outputs |
| Au Y.K., Wynn S.L., Cheung K.M.C. and Cheah K.S.E., functional analysis of SOX9 by conditional expression of a mutant Sox9, Sox9Y440X, in the notochord, 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong.. 2004. |
| Chan D., Ho M.S.P., Tsang K.Y., Chan W.Y., Sillence D., Boot-Handford R., Cheung K.M.C. and Cheah K.S.E., Abnormal chondrocyte differentiation is dosage-dependent in transgenic mice expressing unassembled mutant collagen X in hypertrophic chondrocytes, Human Genome Meeting, Berlin, Germany, April 2004. |
| Chan W.Y., Cheah K.S.E., Murphy G. and Chan D., Role of matrix remodeling in endochondral bone formation, 5th Pan-Pacific Connective Tissue Society Symposium, Ube City, Yamaguchi, Japan, 3-7 June 2003. |
| Cheah K.S.E., Discovering gene function and disease mechanisms through forward and reverse developmental genetics, NUS 8th Postgraduate Congress, 3-5 December 2003. |
| Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., Kwan K.M., Cheung K.M.C., Hunziker E., Bateman J.B. and Chan D., In vivo plasticity in terminal differentiation of hypertrophic chondrocytes induced by ER stress. , Mouse Molecular Genetics Meeting, EMBL, Heidelberg, Germany, September 2003. |
| Cheah K.S.E., Pelling A.L., Kiernan K., Leung K.K.H., Tang A.S.P., Bell D., Lovell-Badge R. and Steel K., Uncovering essential gene function for sensory organ development in the mammalian inner ear, Frontiers in Biomedical Research Symposium, 12 December 2003 Hong Kong. 2003. |
| Cheung K.M.C., Jim J.J.T., Noponen-Hietala N., Ott J., Karppinen J., Luk K.D.K., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Age dependent association of the COL9A2 Trp2 allele with intervertebral disc degeneration, annular tears and end-plate herniations, 38th Annual Meeting of the Scoliosis Research Society, Quebec, Canada, September 10-13, 2003. |
| Cheung K.M.C., Chen Y., Cheah K.S.E., Karppinen J., Leong J.C.Y., Luk K.D.K. and Song Y., Study of the Taq I Polymorphism of Vitamin D Receptor gene in Lumbar Disc Degeneration in Chinese, XVth International Symposium on Services and Local Access. March 2004 . 2004. |
| Cheung K.M.C., Chan W.C.W., Cheah K.S.E. and Chan D., The molecular and cellular changes underlying increased bone formation in a mouse model with generalized progressive hyperostosis, European Cells and Materials. 2003. |
| Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Song Y.Q., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Genetic variation in the COL9A2 gene and its association to intervertebral disc abnormalities in Southern Chinese, Hong Kong Orthopaedic Association, Hong Kong, November 8-9, 2003. |
| Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Luk K.D.K., Yip S.P., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Genetic variation in the Col9A2 gene and its association to intervertebral disc abnormalities in Southern Chinese, XIX International Congress of Genetics, Melbourne, Australia, July 2003. |
| Law S.K.F., Cheah K.S.E., He L., Gao B. and Chan D., The molecular basis of Indian hedgehog mutations in digit patterning, Human Genome Meeting, Berlin, Germany, April 2004. |
| Leung W.L., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) Signalling, 8th Postgraduate Symposium, HKU, December 2003 Hong Kong . 2003. |
| Leung W.L., Wong S.Y.Y., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) signaling., 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June 2004 Hong Kong.. 2004. |
| Li J. and Cheah K.S.E., The role of Indian hedgehog in chondrocyte hypertrophy, 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Li X., Costantino N., Lu L., Liu D., Watt R.M., Cheah K.S.E., Court D.L. and Huang J., Identification of factors influencing strand bias in oligonucleotide-mediated recombination in E. coli., Nucleic Acids Research. 2003, 31(22): 6674-6687. |
| Song Y., Chan D., Karppinen J., Chen Y., Leong J.C.Y., Luk K.D.K., Cheah K.S.E. and Cheung K.M.C., Study of Polymorphisms of Vitamin D Receptor gene in Lumbar Disc Degeneration in Chinese, Human Genome Meeting, Berlin, Germany, April 2004,. 2004, Abstract No. 118. |
| Tai C.P. and Cheah K.S.E., In vivo analysis of the functional specificity of the transcription factor, SOX9, in regulation COL2A1/Col2a1 , 8th Postgraduate Symposium, HKU, December 2003. |
| Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interactions of Hox genes during craniofacial development. , 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Wynn S.L., Au Y.K. and Cheah K.S.E., Defining the role of Sox9 in early development by tissue specific gene inactivation, The 3rd Australian Developmental Biology Workshop, October 2003, Sydney, Australia. 2003. |
| Researcher : Cheng LYL |
| Project Title: | Characterization of rat haemoglobins by immobiline isoelectro-focussing |
| Investigator(s): | Dr. Cheng L.Y.L., Dr. Lam V.M.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/1992 |
| Abstract: |
| To delineate the underlying mechanisms of haemoglobin switching and to characterize the chain composition of various rat haemoglobins. |
| Project Title: | Cloning and characterization of the genomic a-globin gene of rat |
| Investigator(s): | Dr. Cheng L.Y.L., Dr. Lam V.M.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/1993 |
| Abstract: |
| To isolate the genomic a-globin gene and perform restriction mapping with the view to further characterize the control of its expression. |
| Project Title: | A study of the regulatory sequences of the rat b-globin genes |
| Investigator(s): | Dr. Cheng L.Y.L., Dr. Lam V.M.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/1994 |
| Abstract: |
| To characterize the control of b-globin gene expression and to investigate the haemoglobin switching phenomenon. |
| Project Title: | An investigation into the role of the ampullary gland secretions on mammalian fertility |
| Investigator(s): | Dr. Cheng L.Y.L. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/1996 |
| Abstract: |
| To identify and characterize, from the ampullary gland secretions, the protein component(s) that bind to the plasma surface of the spermatazoa. These may play a regulatory role in the control of fertilization in the hamster. The findings may have implications on human fertility. |
| Project Title: | A concordance study of the importance of autosomal short tandem repeat (STR) and Y-chromosome STR in the Chinese community |
| Investigator(s): | Dr. Cheng L.Y.L., Dr. Tan-Un K.C. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2002 |
| Abstract: |
| In recent years, Short Tandem repeat (STR) and microsatellite analyses offer invaluable evidences in forensic science casework in court. Despite the well established database in the US forensic system, an Asian population database urgently needed to be established. In this research study, we use the STR markers that suit the American Combined DNA index system (CODIS) required by FBI to analyse Asian Chinese samples. Hence, the frequency of the allele for each locus can be calculated statistically, and stored for the use of the Chinese community. |
| List of Research Outputs |
| Chen H., Chow P.H., Cheng S.K., Cheung A., Cheng L.Y.L. and O W.S., Male genital tract antioxidant enzymes: their source, funciton in the female, and ability to preserve sperm DNA integrity in the golden hamster, Journal of Andrology. 2003, 24 No. 5: 704-711. |
| Researcher : Chin KT |
| List of Research Outputs |
| Chin K.T. and Jin D., Identification and characterization of a novel liver-enriched transcription factor of the bZIP family, The 6th Conference of Asia-Pacific International Molecular Biology Network (A-IMBN) 2003, Tokyo, Japan, November 2003. . 2003. |
| Chin K.T. and Jin D., Identification and characterization of a novel liver-enriched transcription factor of the bZIP family, Cold Spring Harbor Laboratory 2003 Meeting on Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, New York, August 27-31, 2003. . 2003. |
| Chin K.T. and Jin D., Identification and characterization of a novel liver0enriched transcription factor of the bZIP family , Asia-Pacific International Molecular Biology Network (A-IMBN) The Sixth conference: Dramatic Movement of Life Science: Now to Future, Aomi, Tokyo, Japan, November 12-13, 2003. |
| Researcher : Ching JCY |
| List of Research Outputs |
| Ching J.C.Y., Transcriptional regulation of p16INK4a expression by the forkhead box transcription factor FOXM1, MPhil Thesis. 2003. |
| Researcher : Chun CS |
| List of Research Outputs |
| Chun C.S. and Jin D., p53 represses the transcription of mitotic checkpoint gene MAD1, Asia-Pacific International Molecular Biology Netwrok (A-IMBN) 2003 - The Sixth Conference: Dramatic Movement of Life Science: Now to Future, Aomi, Tokyo, Japan, November 12-13, 2003. |
| Researcher : Gao B |
| List of Research Outputs |
| Law S.K.F., Cheah K.S.E., He L., Gao B. and Chan D., The molecular basis of Indian hedgehog mutations in digit patterning, Human Genome Meeting, Berlin, Germany, April 2004. |
| Researcher : Huang J (Huang Jiandong) |
| Project Title: | Molecular genetic analysis of unconventional myosin 5b (Myo5b): its function and interplay with myosin 5a (Myo5a) |
| Investigator(s): | Dr. Huang J.D., Dr. Copeland N.G., Dr. Jenkins N.A. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2000 |
| Abstract: |
| Within the cell, a variety of cellular components are moved to specific sites at specific times. The various cellular cargoes are carried by different motor molecules such as the myosin and kinesin families of proteins. Mutations in motor proteins result in genetic dieases. The project uses molecular genetics and cell biology approaches to study the function of Myosin 5a (Myo 5a), mutation of which is believed to cause mouse dilute phenotype and human Griscelli's disease. |
| Project Title: | Identification of myosin VA interacting proteins |
| Investigator(s): | Dr. Huang J.D., Prof. Pearlman R.E., Prof. Siu M.K.W., Dr. Wong N.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2001 |
| Abstract: |
| To use biochemical, molecular biology and cell biology techniques to evaluate the candidates that are likely to be the proteins mediating MyoVA binding to the cargoes; to identify more MCP candidates as well as other proteins that are important for MyoVA function. |
| Project Title: | Characterization of the b protein in single-stranded oligonucleotides-mediated recombination |
| Investigator(s): | Dr. Huang J.D. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 10/2002 |
| Completion Date: | 09/2003 |
| Abstract: |
| A detailed biophysical characterization of the b protein; determination of the subcellular localization of b. |
| Project Title: | RNA-DNA hybrid oligonucleotide mediated site-specific repair and gene therapy |
| Investigator(s): | Dr. Huang J.D., Prof. Liu D.P. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 01/2003 |
| Abstract: |
| To establish the reporter system for RDO and SSO mediated mutagenesis in mouse ES cells; to investigate the methods to improve the mutagenesis efficiency of RDO- and SSO-mediated mutagenesis; to study the mechanisms underlying SSO-mediated mutagenesis. |
| Project Title: | DNA recombineering mediated by single stranded oligonucleotides |
| Investigator(s): | Dr. Huang J.D., Prof. Cheah K.S.E., Prof. Liu D.P., Dr. Watt R.M. |
| Department: | Biochemistry |
| Source(s) of Funding: | National Natural Science Foundation of China |
| Start Date: | 03/2003 |
| Abstract: |
| To understand detailed biophysical characterization o the b protein; to identify endogenous proteins essential for Red-mediated recombination in E. coli; to introduce b into mammalian cell lines; to study the mechanism of SSO-mediated mutagenesis in mammalian cells. |
| Project Title: | Characterization of the molecular scaffolds in rab27a and myosin va-mediated vesicular transport |
| Investigator(s): | Dr. Huang J.D., Prof. Miller A., Dr. Sun H.Z. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 07/2003 |
| Abstract: |
| To characterize the vesicles transported by brain Myo Va isoform in Purkinje neurons; to develop a proteomic interactive map for MyoVa isoforms and Rab27 a across range of tissues; to elucidate the mechanism of scaffold assembly/disassembly. |
| Project Title: | Characterization of the molecular scaffolds in rab27a and myosin va-mediated vesicular transport |
| Investigator(s): | Dr. Huang J.D., Prof. Miller A., Dr. Sun H.Z. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 07/2003 |
| Abstract: |
| To characterize the vesicles transported by brain Myo Va isoform in Purkinje neurons; to develop a proteomic interactive map for MyoVa isoforms and Rab27 a across range of tissues; to elucidate the mechanism of scaffold assembly/disassembly. |
| Project Title: | Development of high throughput screen for the discovery of SARS coronavirus helicase inhibitors |
| Investigator(s): | Dr. Huang J.D., Dr. Poon L.L.M., Dr. Sun H.Z., Dr. Tanner J.A., Dr. Watt R.M., |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 10/2003 |
| Completion Date: | 09/2004 |
| Abstract: |
| To carry out biochemical characterization of the putative SARS coronavirus helicase; to evaluate known helicase inhibitors of their efficacy on SARS-CoV nsp10 protein; to develop high throughput assay for the screening of potential helicase inhibitors. |
| List of Research Outputs |
| Ge R., Watt R.M., He Q., Huang J. and Sun H., Towards the Understanding of Nickel Trafficking in Helicobacter pylori: Expression and Brief Characterization of a His-rich Protein, Abstract of The 8th International Symposium on Applied Bioinorganic Chemistry, April 2-5, 2004, Hong Kong. 2004, PP10. |
| Huang J., Unraveling the SARS helicase - from function to therapy, The International workshop on SARS. 2004. |
| Li X., Costantino N., Lu L., Liu D., Watt R.M., Cheah K.S.E., Court D.L. and Huang J., Identification of factors influencing strand bias in oligonucleotide-mediated recombination in E. coli., Nucleic Acids Research. 2003, 31(22): 6674-6687. |
| Sun H., Huang J. and Zheng B., Bismuth as an Enzyme Inhibitor: From Antibacterium to Antivirus, Abstract of The 8th International Symposium on Applied Bioinorganic Chemistry, April 2-5, 2004 . 2004, IL15. |
| Tanner J.A., Watt R.M., Chai Y.B., Lu L.Y., Lin M.C., Peiris J.S., Poon L.L., Kung H. and Huang J., The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases, Journal of Biological Chemistry. 2003, 278(41): 39578-82. |
| Zhang X., Copeland N.G., Jenkins N.A. and Huang J., Cell-specific expression of Cre recombinase in Purkinje and retinal bipolar neurons, 43rd American Society for Cell Biology Annual Meeting. Molecular Biology of the Cell (Suppl). 2003, V14: 406a, 2773-2773. |
| Zhang X. and Huang J., Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method , Nucleic Acids Research. 2003, 31(15): e81. |
| Zhang X., Ng A.H.L., Tanner J.A., Wu W., Jenkins N.A. and Huang J., Highly Restricted Expression of Cre Recombinase in Cerebellar Purkinje Cells, Genesis: The Journal of Genetics and Development. 2004, V40: 45-51. |
| Researcher : Huang J (Huang Jian) |
| List of Research Outputs |
| Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299. |
| Researcher : Jiang Y |
| List of Research Outputs |
| Chin F.Y.L., Leung C.M., Yiu S.M., Lam T.W., Rosenfeld R., Tsang W.W., Smith D.K. and Jiang Y., Finding Motifs for Insufficient Number of Sequences with Strong Binding to Transcription Factor, The Eighth Annual International Conference on Research in Computational Molecular Biology (RECOMB 2004). San Diego, California, USA, 2004, 125-132. |
| Researcher : Jin D |
| Project Title: | Functional characterization of a Novel Kelch repeat protein |
| Investigator(s): | Dr. Jin D.Y., Prof. Yuan J.G. |
| Department: | Biochemistry |
| Source(s) of Funding: | National Natural Science Foundation of China |
| Start Date: | 01/2001 |
| Completion Date: | 12/2003 |
| Abstract: |
| To collaboratively investigate the physiological functions of HC. |
| Project Title: | Implementation of theoretical and technical system for disease genomics |
| Investigator(s): | Dr. Jin D.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | PVC(R)'s China Collaboration Budget |
| Start Date: | 08/2001 |
| Abstract: |
| To study implementation of theoretical and technical system for disease genomics. |
| Project Title: | Characterization of a novel centrosomal target of HTLV-I oncoprotein tax |
| Investigator(s): | Dr. Jin D.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2001 |
| Abstract: |
| To characterize a novel human centrosomal protein, which binds to HTLV-I oncoprotein Tax in yeast two-hybrid assay and co-immunoprecipitates with Tax from extracts of mammalian cells. |
| Project Title: | Characterization of novel cellular proteins targeted by HTLV-1 oncoprotein tax |
| Investigator(s): | Dr. Jin D.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | Concern Foundation |
| Start Date: | 07/2002 |
| Abstract: |
| To characterize TXBP151, a novel cellular protein that binds to HTLV-1 oncoprotein tax. |
| Project Title: | Mitotic checkpoint and genomic stability in ovarian cancer |
| Investigator(s): | Dr. Jin D.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | National Institutes of Health through World Health Organization |
| Start Date: | 10/2002 |
| Abstract: |
| To investigate the molecular basis of mitotic checkpoint in mammalian cells nad its relevance to genomic instability in ovarian cancer. |
| Project Title: | Functional characterization of a novel liver-enriched transcription factor of the bZIP family |
| Investigator(s): | Dr. Jin D.Y., Dr. Ching Y.P., Dr. Chung S.K., Prof. Ng I.O.L. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2002 |
| Abstract: |
| Previous study of the research team shows that LZIP-b is a liver-enriched transcription factor implicated in tissue-specific expression of genes. In this study, the team will focus on four areas of research:- 1) biochemical and biological characterization of LZIP-b transcript and protein in mammalian tissues and cells; 2) functional characterization of LZIP-b as a novel CRE-binding transcription factor; 3) investigating the roles of LZIP-b in liver-specific gene expression and cancer development; and 4) establishment of frog and mouse models for functional studies of LZIP-b. |
| Project Title: | Development of novel nucleic acid therapeutics for viral infection and cancer |
| Investigator(s): | Dr. Jin D.Y., Prof. Chan L.C., Dr. Ko C.B., Prof. Lai C.L., Prof. Ng I.O.L., Dr. Sham M.H., Dr. Smith D.K., Prof. Tsao G.S.W. |
| Department: | Biochemistry |
| Source(s) of Funding: | Innovation and Technology Fund, Innovation and Technology Commission of Hong Kong Government |
| Start Date: | 06/2003 |
| Abstract: |
| To further optimize the protocols for computer-aided design of siRNAs/ shRNAs and for mass production of siRNAs through in vitro transcription catalyzed by T7/SP6 RNA polymerase; to develop new and improved vectors for delivery of shRNA into human cells; to design, produce and test more than 50 siRNAs and shRNAs targeting hepatitis B and C viruses, lymphoma and leukemia, as well as liver cancer; to develop and further improve various indicator plasmid constructs, cell culture systems and animal models for evaluating the effectiveness of nucleic acid therapeutics on hepatitis, liver cancer, lymphoma and leukemia. |
| Project Title: | Development of RNAi technology for inhibition of viral replication |
| Investigator(s): | Dr. Jin D.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | Run Run Shaw Research and Teaching Endowment Fund |
| Start Date: | 11/2003 |
| Abstract: |
| To use Sindbis virus as a model to systematically study RNAi-mediated inhibition of viral replication; to study the rules for selection of RNAi targets particularly effective for inhibition of viral replication in mammalian cells; to compare the effectiveness of siRNAs targeting UTR, non-structural (such as polymerase) or structural (such as capsid) regions. |
| Project Title: | Roles and regulation of peroxiredoxins: from yeast to human |
| Investigator(s): | Dr. Jin D.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2003 |
| Abstract: |
| To perform in-depth analyses on the transcriptional regulation of yeast PMP20; to study the structure-function relationship of yeast and human peroxiredoxins; to characterize the impact of binding to heme on Tsa1p/Tsa2p function; to investigate the roles of peroxiredoxins in cell physiology and pathology. Out focus will be on apoptosis, aging and cell proliferation. |
| Project Title: | Roles and regulation of peroxiredoxins: from yeast to human |
| Investigator(s): | Dr. Jin D.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 12/2003 |
| Abstract: |
| To perform in-depth analyses on the transcriptional regulation of yeast PMP20; to study the structure-function relationship of yeast and human peroxiredoxins; to characterize the impact of binding to heme on Tsa1p/Tsa2p function; to investigate the roles of peroxiredoxins in cell physiology and pathology. Out focus will be on apoptosis, aging and cell proliferation. |
| List of Research Outputs |
| Chan C.F., Yau T.O., Jin D., Wong C.M., Fan S.T. and Ng I.O.L., Evaluation of nuclear factor-kB, urokinase-type plasminogen activator, and HBx and their clinicopathological significance in hepatocellular carcinoma, Clinical Cancer Research. 2004, 10(12 pt. 1): 4140-4149. |
| Chin K.T. and Jin D., Identification and characterization of a novel liver-enriched transcription factor of the bZIP family, The 6th Conference of Asia-Pacific International Molecular Biology Network (A-IMBN) 2003, Tokyo, Japan, November 2003. . 2003. |
| Chin K.T. and Jin D., Identification and characterization of a novel liver-enriched transcription factor of the bZIP family, Cold Spring Harbor Laboratory 2003 Meeting on Mechanisms of Eukaryotic Transcription, Cold Spring Harbor, New York, August 27-31, 2003. . 2003. |
| Chin K.T. and Jin D., Identification and characterization of a novel liver0enriched transcription factor of the bZIP family , Asia-Pacific International Molecular Biology Network (A-IMBN) The Sixth conference: Dramatic Movement of Life Science: Now to Future, Aomi, Tokyo, Japan, November 12-13, 2003. |
| Ching Y.P., Leong V.Y.L., Jin D. and Ng I.O.L., Regulation of the tumour metastasis suppressor gene, NM23, by p21-activated protein kinase 5., Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004. |
| Chun C.S. and Jin D., p53 represses the transcription of mitotic checkpoint gene MAD1, Asia-Pacific International Molecular Biology Netwrok (A-IMBN) 2003 - The Sixth Conference: Dramatic Movement of Life Science: Now to Future, Aomi, Tokyo, Japan, November 12-13, 2003. |
| Huang G.J., Zhang Z.Q., Yao L.H., Chen A.J., Xu C.X., Zju H.J., Chai Y.S., Yan X.R. and Jin D., Yeast two-hybrid screening for new binding partners of CIKS, Chinese Journal of Biotechnology. 2003, 19(2): 190-194. |
| Jin D., A centrosomal target of human T-cell leukemia virus type I oncoprotein Tax, American Society for Virology 22nd Annual Meeting, Davis, California, July 12-16, 2003.. 2003. |
| Jin D., Human T-cell leukemia virus type I oncoprotein Tax targets the centrosome, The 2003 Stohlman Scholar Symposium, Cleveland, Ohio, November 6-7, 2003.. 2003. |
| Kok K.H. and Jin D., RNAi in Xenopus embryos , In: Sohail, M., Gene Silencing by RNA Interference: Technology and application . Boca Ration, CRC Press, 2004, 231-240. |
| Kok K.H. and Jin D., Rational design and development of small interfering RNAs and small hairpin RNAs for inhibition of hepatitis C virus replication (oral presentation W1-7), American Society for Virology 22nd Annual Meeting, Davis, California, July 12-16, 2003.. 2003. |
| Leung H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Characterization of novel putative tumor suppressor gene, DLC2 (deleted in liver cancer 2), in hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004. |
| Li H.M., Zhuang Z., Wang Q., Pang J.C.S., Wang X., Wong H.L., Feng H., Jin D., Ling M.T., Wong Y.C., Eliopoulos A.G., Young L.S., Huang D.P. and Tsao G.S.W., Epstein-Barr virus latent membrane protein 1 (LMP1) upregulates Id1 expression in nasopharyngeal epithelial cells, Oncogene. 2004, 1-7. |
| Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Dysregulation of mitotic spindle checkpoint control in hepatocellular carcinomas, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004. |
| Wong C.M., Ching Y.P., Lee M.F., Jin D. and Ng I.O.L., Genetic and epigenetic alterations of DLC-1 gene in hepatocellular carcinoma, Cancer Research. 2003, 63: 7646-7651. |
| Wong C.M., Siu K.L. and Jin D., Peroxiredoxin-null yeast cells are hypersensitive to oxidative stress and genomially unstable, Journal of Biological Chemistry. 2004, 279: 23207-23213. |
| Yam J.W.P., Jin D., So C.W. and Chan L.C., Identification and characterization of EBP, a novel EEN binding protein that inhibits Ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein, Blood. 2004, 103(4): 1445-1453. |
| Researcher : Kok KH |
| List of Research Outputs |
| Kok K.H. and Jin D., RNAi in Xenopus embryos , In: Sohail, M., Gene Silencing by RNA Interference: Technology and application . Boca Ration, CRC Press, 2004, 231-240. |
| Kok K.H. and Jin D., Rational design and development of small interfering RNAs and small hairpin RNAs for inhibition of hepatitis C virus replication (oral presentation W1-7), American Society for Virology 22nd Annual Meeting, Davis, California, July 12-16, 2003.. 2003. |
| Researcher : Kwok C |
| List of Research Outputs |
| Kwok C., A study of quaternary structure of human G6PD, PhD Thesis. 2003. |
| Researcher : Kwok JCF |
| List of Research Outputs |
| Kwok J.C.F., Ng K.Y., Zhang F., Shum D.K.Y. and Chan Y.S., Chondroitin sulfates restrict axonal growth along the projection path of VN neurons across the hindbrain of prenatal Sprague-Dawley rats, The Annual Meeting of the Hong Kong Neuroscience Society, 9-10 Dec, 2003, Faculty of Medicine, HKU . 2003. |
| Kwok J.C.F., Expression of chondroitin sulfates in the developing hindbrain-contributions to plasticity, PhD Thesis. 2004. |
| Kwok J.C.F., Spatiotemporal Relationship Between Chondroitin Sulfates And Chondroitin 6-sulfotransferase In The Neuroepithelium During Mouse Hindbrain Development, The Second HKU-Cambridge Retreat, March 2004, aculty of Medicine, HKU . 2004. |
| Kwok J.C.F., Ng K.Y., Shum D.K.Y. and Chan Y.S., Tracking commissural projections of vestibular neurons in a chondroitin, sulfate-enriched environment of the developing hindbrain , The Gordon Research Conference on Molecular & Cellular, June 6-11, 2004,HKUST, Hong Kong . 2004. |
| Researcher : Lam VMS |
| Project Title: | Structural NADP+, subunit interaction and other stability determinants in human glucose-6-phosphate dehydrogenase (G6PD) and deficient variants |
| Investigator(s): | Dr. Lam V.M.S., Dr. Adams M.J., Prof. Engel P.C. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 11/2000 |
| Abstract: |
| Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a global and local of jaundice in newborns. The majority are usually asymptomatic but environmental agents such as the ingestion of fava bean or some Chinese herbal medicines can induce favism or haemolysis. The project aims to bioengineer change(s) in the amino acids, around the 'structural' NADP site, some of which correspond to naturally occurring Class 1 variants. |
| Project Title: | The C-terminus of human glucose-6-phosphate dehydrogenase (h-G6PD): a structural and/or regulatory domain? |
| Investigator(s): | Dr. Lam V.M.S., Dr. Adams M.J., Prof. Engel P.C. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2002 |
| Abstract: |
| The project aims at examining the role of the aromatic amino acids in the C-terminus. The research team will create a series of mutations in each of these residues, express and purify these enzymes for assay of stability and NADP+ binding. |
| Project Title: | Protein folding and glucose-6-phosphate dehyrogenase (G6PD) deficiency |
| Investigator(s): | Dr. Lam V.M.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 01/2003 |
| Completion Date: | 12/2003 |
| Abstract: |
| Construct recombinants in pET30 which can over-express mutant proteins G163S and G163D; experiment with temperatures and co-expression with chaperonins to produce the mutant proteins; produce h-G6PD specific monoclonal antibodies; use the h-G6PD specific antibodies to evaluate the protein(s) synthesised under (2) above; experiment with pulse-label to follow the fate of the synthesised mutant protein(s). |
| Project Title: | A study on the roles of the amino acids involved in the catalytic efficiency of human glucose-6-phosphate dehydrogenase (G6PD) |
| Investigator(s): | Dr. Lam V.M.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 02/2004 |
| Abstract: |
| To construct recombinants which can express R72Q and R72A mutant enzymes, in different vectors; take experiment to express the enzymes under different conditions; to develop different strategies to purify the enzymes; ADP-sepharose column is likely to be ineffective; to obtain initial rate measurements and determine the kinetic and/or NADP+ binding constants of these enzymes. |
| List of Research Outputs |
| Lam V.M.S., Au D.M.Y. and Chan L.C., Multiplex detection: a rapid and reliable method to screen common variations in the Glucose-6-phosphate dehydrogenase gene. , Mutation Detection Workshop, satellite meeting of the International Congress of Genetics, July, Australia.. 2003. |
| Researcher : Law SKF |
| List of Research Outputs |
| Law S.K.F., Cheah K.S.E., He L., Gao B. and Chan D., The molecular basis of Indian hedgehog mutations in digit patterning, Human Genome Meeting, Berlin, Germany, April 2004. |
| Researcher : Leong JCY |
| Project Title: | Biomechanical evaluation of the human sacrum for lumbosacral fixation |
| Investigator(s): | Prof. Leong J.C.Y., Dr. Lu W.W., Prof. Zhong S.Z., Dr. Zhu Q.A. |
| Department: | Orthopaedics and Traumatology |
| Source(s) of Funding: | Departmental Budget |
| Start Date: | 08/1995 |
| Abstract: |
| Using in vitro method to find out whether fusion to the sacrum only is strong enough for lumbosacral fixation, and to determine where the best possible regions for screw insertion. |
| Project Title: | The role of the pineal gland in the causation of scoliosis: a bipedal rhesus monkey model |
| Investigator(s): | Prof. Leong J.C.Y., Prof. Carl A., Dr. Cheung K.M.C., Prof. Hu Y.G., Prof. Pang S.F. |
| Department: | Orthopaedics and Traumatology |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/1999 |
| Abstract: |
| To find out one possible cause for adolescent idiopathic scoliosis. |
| List of Research Outputs |
| Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Luk K.D.K., Yip S.P., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Genetic variation in the Col9A2 gene and its association to intervertebral disc abnormalities in Southern Chinese, XIX International Congress of Genetics, Melbourne, Australia, July 2003. |
| Researcher : Leong VYL |
| List of Research Outputs |
| Ching Y.P., Leong V.Y.L., Jin D. and Ng I.O.L., Regulation of the tumour metastasis suppressor gene, NM23, by p21-activated protein kinase 5., Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004. |
| Researcher : Leung KKH |
| List of Research Outputs |
| Cheah K.S.E., Pelling A.L., Kiernan K., Leung K.K.H., Tang A.S.P., Bell D., Lovell-Badge R. and Steel K., Uncovering essential gene function for sensory organ development in the mammalian inner ear, Frontiers in Biomedical Research Symposium, 12 December 2003 Hong Kong. 2003. |
| Researcher : Leung WL |
| List of Research Outputs |
| Leung W.L., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) Signalling, 8th Postgraduate Symposium, HKU, December 2003 Hong Kong . 2003. |
| Leung W.L., Wong S.Y.Y., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) signaling., 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June 2004 Hong Kong.. 2004. |
| Researcher : Li H |
| List of Research Outputs |
| Chau C.H., Shum D.K.Y., Li H., Pei J., Lui Y.Y., Wirthlin L., Chan Y.S. and Xu X.M., Chondronitase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury, FASEB Journal. 2004, 18(1): 194-196. |
| Researcher : Li J |
| List of Research Outputs |
| Li J. and Cheah K.S.E., The role of Indian hedgehog in chondrocyte hypertrophy, 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Researcher : Li X |
| List of Research Outputs |
| Li X., Costantino N., Lu L., Liu D., Watt R.M., Cheah K.S.E., Court D.L. and Huang J., Identification of factors influencing strand bias in oligonucleotide-mediated recombination in E. coli., Nucleic Acids Research. 2003, 31(22): 6674-6687. |
| Researcher : Liu D |
| List of Research Outputs |
| Li X., Costantino N., Lu L., Liu D., Watt R.M., Cheah K.S.E., Court D.L. and Huang J., Identification of factors influencing strand bias in oligonucleotide-mediated recombination in E. coli., Nucleic Acids Research. 2003, 31(22): 6674-6687. |
| Researcher : Lu L |
| List of Research Outputs |
| Li X., Costantino N., Lu L., Liu D., Watt R.M., Cheah K.S.E., Court D.L. and Huang J., Identification of factors influencing strand bias in oligonucleotide-mediated recombination in E. coli., Nucleic Acids Research. 2003, 31(22): 6674-6687. |
| Researcher : Lui YY |
| List of Research Outputs |
| Chau C.H., Shum D.K.Y., Li H., Pei J., Lui Y.Y., Wirthlin L., Chan Y.S. and Xu X.M., Chondronitase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury, FASEB Journal. 2004, 18(1): 194-196. |
| Researcher : Ma RYM |
| List of Research Outputs |
| Ma R.Y.M., The mitogen-activated protein kinase pathway regulates the subcellular localization and function of FOXM, MPhil Thesis. 2003. |
| Researcher : Ng DCH |
| List of Research Outputs |
| Leung H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Characterization of novel putative tumor suppressor gene, DLC2 (deleted in liver cancer 2), in hepatocellular carcinoma, Proceedings of the 95th Annual Meeting of American Association for Cancer Research, Orlando, Florida, USA, March 2004. |
| Researcher : Ott J |
| List of Research Outputs |
| Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Luk K.D.K., Yip S.P., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., Genetic variation in the Col9A2 gene and its association to intervertebral disc abnormalities in Southern Chinese, XIX International Congress of Genetics, Melbourne, Australia, July 2003. |
| Researcher : Pelling AL |
| List of Research Outputs |
| Cheah K.S.E., Pelling A.L., Kiernan K., Leung K.K.H., Tang A.S.P., Bell D., Lovell-Badge R. and Steel K., Uncovering essential gene function for sensory organ development in the mammalian inner ear, Frontiers in Biomedical Research Symposium, 12 December 2003 Hong Kong. 2003. |
| Researcher : Sae-Pang JJ |
| List of Research Outputs |
| Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interactions of Hox genes during craniofacial development. , 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Researcher : Sham MH |
| Project Title: | Studying the roles of endophilins, a family of SH3 domain containing proteins, in mouse development by gene targeting |
| Investigator(s): | Dr. Sham M.H., Prof. Chan L.C., Dr. So C.W. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2000 |
| Abstract: |
| To study the role of the family of endophilin genes during mammalian development, from embryonic stages to adult. |
| Project Title: | Analysis of the abnormal phenotypes of a Hoxb3lacZ mutant allele generated by gene targeting |
| Investigator(s): | Dr. Sham M.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 10/2002 |
| Completion Date: | 09/2003 |
| Abstract: |
| To analyse the phenotype of the Hoxb3lacZ mutant during embryogenesis in order to understand how alternations of Hoxb3 gene transcription might affect cardiovascular development and function. |
| Project Title: | Hoxb3lacZ: a mutant mouse model for studying the roles of Hoxb3 in heart and thoracic body wall development |
| Investigator(s): | Dr. Sham M.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2003 |
| Abstract: |
| To characterize the Hoxb3(lacZ) mutant locus and examine if this is a hypomorphic mutant; to analyse the cardiac abnormalities in the Hoxb3(lacZ) mutant using molecular markers and determine the role of Hoxb3 during heart development; to examine the ventral body wall defect by morphological and molecular markers, and identify any cooperation of Hoxb3 with other Hox genes in the developmental processes involved. |
| Project Title: | Hoxb3lacZ: a mutant mouse model for studying the roles of Hoxb3 in heart and thoracic body wall development |
| Investigator(s): | Dr. Sham M.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 09/2003 |
| Abstract: |
| To characterize the Hoxb3(lacZ) mutant locus and examine if this is a hypomorphic mutant; to analyse the cardiac abnormalities in the Hoxb3(lacZ) mutant using molecular markers and determine the role of Hoxb3 during heart development; to examine the ventral body wall defect by morphological and molecular markers, and identify any cooperation of Hoxb3 with other Hox genes in the developmental processes involved. |
| Project Title: | Mapping the gene for a novel blind mouse mutant |
| Investigator(s): | Dr. Sham M.H., Dr. Song Y.Q. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 02/2004 |
| Abstract: |
| To identify the mutation that leads to the blind phenotype of the mutant mouse strain we produced by genome-wide mapping using microsatellite markers. |
| List of Research Outputs |
| Cai K., Sham M.H., Zheng D.X. and Xu R., Anti-HBV drug and treatment, Chinese Journal of Medicine. 2003, 50: 744-746. |
| Chan K.K., Chen A.Y.S., Lui V.C.H., Tam P.K.H. and Sham M.H., Abnormal enteric ganglia development in transgenic mice with ectopic expression of mutant Sox10 in the vagal neural crest. , The 4th International Meeting on Hirschprung Disease and Related Neurocristopathies HSCR, April 2004, Genova italy. 2004. |
| Chan K.K., Wong C.K.Y., Lui V.C.H., Tam P.K.H. and Sham M.H., Analysis of SOX10 mutations identified in Waardenburg-Hirschsprung patients: differential effects on target gene regulation, Journal of Cellular Biochemistry. 2003, 90(3): 573-585. |
| Fu M., Tam P.K.H., Sham M.H. and Lui V.C.H., Embryonic development of the ganglion plexuses and the concentric layer structure of human gut: a topographical study, Anatomy and Embryology. 2004, 208(1): 33-41. |
| Fu M., Lui V.C.H., Sham M.H., Cheung A.N.Y. and Tam P.K.H., HOXB5 expression is spatially and temporarily regulated in human embryonic gut during neural crest cell colonization and differentiation of enteric neuroblasts, Developmental Dynamics. 2003, 228(1): 1-10. |
| Garcia-Barcelo M.M., Sham M.H., Lui V.C.H., Chen B.L.S., Song Y., Lee W.S., Yung S.K., Romeo G. and Tam P.K.H., Chinese patients with sporadic Hirschsprung’s disease are predominantly represented by a single RET haplotype. , Journal of Medical Genetics. 2003, 40(11): e122. |
| Garcia-Barcelo M.M., Sham M.H., Lee W.S., Lui V.C.H., Chen B.L.S., Wong K.K.Y., Wong J.S.W. and Tam P.K.H., Highly recurrent RET mutations and novel mutations in genes of the receptor tyrosine kinase and endothelin receptor B pathways in Chinese patients with sporadic Hirschsprung disease, Clinical Chemistry. 2004, 50(1): 93-100. |
| Garcia-Barcelo M.M., Lui V.C.H., Sham M.H. and Tam P.K.H., Is there a role for Hedgehog genes in Hirschsprung's diseases?, 37th Annual Meeting of the Pacific Association of Pediatric Surgeons, Seoul, Korea, 16-20 May, 2004. |
| Garcia-Barcelo M.M., Lee W.S., Sham M.H., Lui V.C.H. and Tam P.K.H., Is there a role for the IHH gene in Hirschsprung's disease?, Neurogastroenterology and Motility. 2003, 15(6): 663-668. |
| Kong C.T., Chan L.C. and Sham M.H., Mll-Een affects early lineage commitment of embryonic stem cells and cause maturation arrest of myeloid progenitors, FASEBSummer Research Conference on Hematologic Malignancies 26-31 July 2003, Vermount, USA. 2003. |
| Lee K.Y., Samy E.T., Sham M.H., Tam P.K.H. and Lui V.C.H., 3' Splicing variants of ret receptor tyrosine kinase are differentially expressed in mouse embryos and in adult mice, Biochimica Biophysica Acta-Gene Structure Expression. 2003, 1627: 26-38. |
| Leung T.W.C., Cheung H.W., Bhatia I., Yip H.K.F., Sham M.H. and Cheung P.T., Characterization of a novel X-linked nuclear protein down-regulated in neonatal mouse hypoxic-ischemic encephalopathy, HID1, Journal of Neurochemistry. UK, Blackwell Publishing Ltd, 2003, 87 Suppl.: 143-143. |
| Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interactions of Hox genes during craniofacial development. , 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Researcher : Shum DKY |
| Project Title: | Post-traumatic axonal regeneration in glycosaminoglycan-modified spinal cord environments |
| Investigator(s): | Dr. Shum D.K.Y., Prof. Chan Y.S., Dr. Xu X.M. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 12/2000 |
| Abstract: |
| To determine, using an interdisciplinary approach, when and where chondroitin sulphate proteoglycan expression becomes inhibitory to axon regrowth across the graft/host interfaces so that modulatory strategies can be targeted at this critical phase. The project will lead to improved understanding on the functional recovery of patients with spinal cord injury. |
| Project Title: | The role of chondroitin 6-sulfotransferase in post-traumatic regeneration of peripheral nerves |
| Investigator(s): | Dr. Shum D.K.Y., Prof. Chan Y.S., Dr. Chung S.K. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 10/2001 |
| Abstract: |
| To identify the cell type(s) that produce the CS moiety in question; to determine the time course and expression pattern of C6ST in relation to axon regrowth and conduction across the crush-injured nerve; to determine if suppression of C6ST changes the efficacy of regeneration of crushed nerves. |
| Project Title: | Expression of heparanase in the injured spinal cord |
| Investigator(s): | Dr. Shum D.K.Y. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2003 |
| Abstract: |
| To determine the time course and cellular source of heparanase expression in the bridged hemicord; to determine the distribution of substrate HS in relation to the heparanase-expressing cells. |
| List of Research Outputs |
| Chan C.H., Shum D.K.Y. and Ip M.S.M., Sputum sol neutrophil elastase activity in bronchiectasis: differential modulation by syndecan-1 , American Journal of Respiratory and Critical Care Medicine. 2003, 168(2): 192-198. |
| Chan Y.S., Lai C.H., Shum D.K.Y., Yung K.K.L. and Zhang F., Receptors of glutamate and neurotrophin in vestibular neuronal functions, Journal of Biomedical Science. 2003, 10: 577-587. |
| Chau C.H., Shum D.K.Y., Li H., Pei J., Lui Y.Y., Wirthlin L., Chan Y.S. and Xu X.M., Chondronitase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury, FASEB Journal. 2004, 18(1): 194-196. |
| Ho J.C.M., Tipoe G.L., Zheng L., Leung T.M., Tsang K.W.T., Shum D.K.Y., Lau W.C.S., Mak J.C.W., Lam W.K. and Ip M.S.M., In vitro study of regulation of IL-6 production in bronchiectasis, Respiratory Medicine. 2004, 98: 334-341. |
| Kwok J.C.F., Ng K.Y., Zhang F., Shum D.K.Y. and Chan Y.S., Chondroitin sulfates restrict axonal growth along the projection path of VN neurons across the hindbrain of prenatal Sprague-Dawley rats, The Annual Meeting of the Hong Kong Neuroscience Society, 9-10 Dec, 2003, Faculty of Medicine, HKU . 2003. |
| Kwok J.C.F., Ng K.Y., Shum D.K.Y. and Chan Y.S., Tracking commissural projections of vestibular neurons in a chondroitin, sulfate-enriched environment of the developing hindbrain , The Gordon Research Conference on Molecular & Cellular, June 6-11, 2004,HKUST, Hong Kong . 2004. |
| Lai C.H., Tse Y.C., Shum D.K.Y., Yung K.K. and Chan Y.S., Fos expression in central otolith neurons of postnatal rats following off-vertical axis rotation, Journal of Comparative Neurology. 2004, 470: 282-296. |
| Shum D.K.Y. and Ip M.S.M., Degradation of lung matrix proteoglycans in bronchiectasis, In: HG Garg, PJ roughley and CA Hales , Proteoglycans and Lung Disease in "Lung Biology in Health and Disease" Series. New York, Marcel Dekker, 2003, 323-333. |
| Wat A.L.O., Shum D.K.Y. and Ip M.S.M., Characterization of heparan sulphate proteoglycan recovered from cultures pf human bronchial epithelial cells, 8th Research Postgraduate Symposium, Faculty of Medicine, HKU. 2003, Abstract Book: p.68. |
| Zhang F., Ng K.Y., Shum D.K.Y. and Chan Y.S., Axonal trajectories of the vestibular nuclei in the developing rat embryo, Neuroscience Letters Supplement. 2003, 58: S23. |
| Researcher : Siu KL |
| List of Research Outputs |
| Wong C.M., Siu K.L. and Jin D., Peroxiredoxin-null yeast cells are hypersensitive to oxidative stress and genomially unstable, Journal of Biological Chemistry. 2004, 279: 23207-23213. |
| Researcher : Smith DK |
| Project Title: | Patterns in proteins with unusual flexibility profiles |
| Investigator(s): | Dr. Smith D.K. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 08/2002 |
| Abstract: |
| To identify what makes some proteins imcompatible with flexibility parameters calculated from a large set of protein structures. |
| Project Title: | Distinguishing among functional, chance occurrences and variations in the DNA sequences bound by transcription factors |
| Investigator(s): | Dr. Smith D.K., Dr. Yao K.M. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 07/2003 |
| Abstract: |
| To improve computationally based methods for the detection of transcription factor binding sites in genomic DNA and to distinguish whether slight variations in binding site preference among structurally related transcription factors represent functionally important differences. |
| List of Research Outputs |
| Chin F.Y.L., Leung C.M., Yiu S.M., Lam T.W., Rosenfeld R., Tsang W.W., Smith D.K. and Jiang Y., Finding Motifs for Insufficient Number of Sequences with Strong Binding to Transcription Factor, The Eighth Annual International Conference on Research in Computational Molecular Biology (RECOMB 2004). San Diego, California, USA, 2004, 125-132. |
| Radivojac P., Obradovic Z., Smith D.K., Zhu G., Vucetic S., Brown C., Lawson J.D. and Dunker A.K., Protein flexibility and intrinsic disorder, Protein Science. 2004, 13(1): 71-80. |
| Researcher : Tai CP |
| List of Research Outputs |
| Tai C.P. and Cheah K.S.E., In vivo analysis of the functional specificity of the transcription factor, SOX9, in regulation COL2A1/Col2a1 , 8th Postgraduate Symposium, HKU, December 2003. |
| Researcher : Tam CW |
| List of Research Outputs |
| Tam C.W., Combating prostate disease with ethnobotanical drug: inhibition of prostate cancer cell proliferation by saw palmetto (Serernoa repens) extracts, MPhil Thesis. 2003. |
| Researcher : Tam SCF |
| List of Research Outputs |
| Au W.Y., Tsang J., Cheng T.S..., Chow W.S., Woo Y.C., Ma E.S.K. and Tam S.C.F., Cough mixture abuse as a novel cause of megaloblastic anemia and peripheral neuropathy, British Journal of Haematology. 2003, 123: 956-958. |
| But B., Chan C.W., Chan F., Chan K.W., Cheng A.W.F., Cheung P., Choi K.L., Chow C.B., Chow F.C.C., Eastman C., Fok T.F., Fung L.M., Gomes C., Huen K.F., Ip T.P., Kung A.W.C., Lam K.S.L., Lam Y.Y., Lao T.T.H., Lee C.Y., Lee K.F., Leung J., Leung N.K., Li D., Li J., Lo K.W., Low L.C.K., Ng K.L., Siu S.C., Tam S.C.F., Tan K.C.B., Tiu S.C., Tse H.Y., Tse W., Wong G., Wong S., Wong W., Yeung V.T.F., Young R., Yu C.M. and Yu R., Consensus statement on iodine deficiency disorders in Hong Kong, Hong Kong Medical Journal. 2003, 9: 446-453. |
| Cheung Y.F., Yung T.C., Tam S.C.F., Ho M.H.K. and Chau A.K.T., Novel and Traditional Cardiovascular Risk Factors in Children After Kawasaki Disease. Implications for Premature Atherosclerosis, Journal of the American College of Cardiology. Elsevier Inc., 2004, 43(1): 120-124. |
| Cheung Y.F., Yung T.C., Tam S.C.F., Ho M.H.K. and Chau A.K.T., Novel and Traditional Cardiovascular Risk Factors in Children After Kawasaki Disease: Implications for Premature Atherosclerosis, The Seventh Annual Scientific Meeting of Institute of Cardiovascular Science and Medicine, HKU (abstract published in Journal of the Hong Kong College of Cardiology, Vol 11 (Suppl 1: A7), 6-7 December 2003. |
| Cheung Y.F., Yung T.C., Tam S.C.F., Ho M.H.K. and Chau A.K.T., Novel and Traditional Cardiovascular Risk Factors in Children after Kawasaki Disease: Implications for Premature Atherosclerosis, Eleventh Annual Scientific Congress of Hong Kong College of Cardiology (Abstract published in Journal of Hong Kong College of Cardiology), HK, 31 October - 2 November 2003. 11: 133. |
| Cheung Y.F., Ho M.H.K., Yung T.C., Tam S.C.F. and Chau A.K.T., Novel and traditional cardiovascular risk factors in children after Kawasaki disease: Implications for premature atherosclerosis, Joint Annual Scientific Meeting 2003 of the Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association Limited, (The second best oral presentation), QEH, Hong Kong, 13 December 2003. 3-4. |
| Researcher : Tang ASP |
| List of Research Outputs |
| Cheah K.S.E., Pelling A.L., Kiernan K., Leung K.K.H., Tang A.S.P., Bell D., Lovell-Badge R. and Steel K., Uncovering essential gene function for sensory organ development in the mammalian inner ear, Frontiers in Biomedical Research Symposium, 12 December 2003 Hong Kong. 2003. |
| Tang A.S.P., Thesis title Molecular Developmental Genetics of the Inner Ear mutant, Yellow submarine (Ysb), PhD Thesis. 2003. |
| Researcher : Tanner JA |
| List of Research Outputs |
| Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299. |
| Tanner J.A., Watt R.M., Chai Y.B., Lu L.Y., Lin M.C., Peiris J.S., Poon L.L., Kung H. and Huang J., The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases, Journal of Biological Chemistry. 2003, 278(41): 39578-82. |
| Zhang X., Ng A.H.L., Tanner J.A., Wu W., Jenkins N.A. and Huang J., Highly Restricted Expression of Cre Recombinase in Cerebellar Purkinje Cells, Genesis: The Journal of Genetics and Development. 2004, V40: 45-51. |
| Researcher : Tsang KY |
| List of Research Outputs |
| Chan D., Ho M.S.P., Tsang K.Y., Chan W.Y., Sillence D., Boot-Handford R., Cheung K.M.C. and Cheah K.S.E., Abnormal chondrocyte differentiation is dosage-dependent in transgenic mice expressing unassembled mutant collagen X in hypertrophic chondrocytes, Human Genome Meeting, Berlin, Germany, April 2004. |
| Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., Kwan K.M., Cheung K.M.C., Hunziker E., Bateman J.B. and Chan D., In vivo plasticity in terminal differentiation of hypertrophic chondrocytes induced by ER stress. , Mouse Molecular Genetics Meeting, EMBL, Heidelberg, Germany, September 2003. |
| Researcher : Tsang SL |
| List of Research Outputs |
| Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interactions of Hox genes during craniofacial development. , 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Researcher : Wat ALO |
| List of Research Outputs |
| Wat A.L.O., Shum D.K.Y. and Ip M.S.M., Characterization of heparan sulphate proteoglycan recovered from cultures pf human bronchial epithelial cells, 8th Research Postgraduate Symposium, Faculty of Medicine, HKU. 2003, Abstract Book: p.68. |
| Researcher : Watt RM |
| List of Research Outputs |
| Ge R., Watt R.M., He Q., Huang J. and Sun H., Towards the Understanding of Nickel Trafficking in Helicobacter pylori: Expression and Brief Characterization of a His-rich Protein, Abstract of The 8th International Symposium on Applied Bioinorganic Chemistry, April 2-5, 2004, Hong Kong. 2004, PP10. |
| Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299. |
| Li X., Costantino N., Lu L., Liu D., Watt R.M., Cheah K.S.E., Court D.L. and Huang J., Identification of factors influencing strand bias in oligonucleotide-mediated recombination in E. coli., Nucleic Acids Research. 2003, 31(22): 6674-6687. |
| Tanner J.A., Watt R.M., Chai Y.B., Lu L.Y., Lin M.C., Peiris J.S., Poon L.L., Kung H. and Huang J., The severe acute respiratory syndrome (SARS) coronavirus NTPase/helicase belongs to a distinct class of 5' to 3' viral helicases, Journal of Biological Chemistry. 2003, 278(41): 39578-82. |
| Researcher : Wong BCW |
| List of Research Outputs |
| Mangan J.K., Rane S.G., Kang A.D., Amanullah A., Wong B.C.W. and Reddy E.P., Mechanisms associated with IL-6-induced up-regulation of Jak3 and its role in monocytic differentiation. , Blood. 2004, 103(11): 4093-101. |
| Researcher : Wong CM |
| List of Research Outputs |
| Wong C.M., Siu K.L. and Jin D., Peroxiredoxin-null yeast cells are hypersensitive to oxidative stress and genomially unstable, Journal of Biological Chemistry. 2004, 279: 23207-23213. |
| Researcher : Wong EYM |
| List of Research Outputs |
| Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interactions of Hox genes during craniofacial development. , 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong. . 2004. |
| Researcher : Wong IHN |
| Project Title: | Epigenetic and genetic deregulation in association with the Ras signaling pathway in childhood neoplasia |
| Investigator(s): | Dr. Wong I.H.N., Prof. Tam P.K.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 10/2002 |
| Completion Date: | 09/2003 |
| Abstract: |
| To study the transcriptional deregulation of ARF, NF-1, and RASSFIA in childhood solid neoplasms and pediatric cancer cell lines; to investigate whether transcriptional repression of ARF, NF-1, and RASSFIA is caused by epigenetic mechanisms, pediatric cancer cell lines will be treated with a demethylating drug and an inhibitor of histone deacetylase in order to reactivate the expression of these three critical tumor suppressor genes; to identify epigenetic and genetic alterations in ARF, NF-1, and RASSF1 associated with the Ras signaling or Ras effector pathways in childhood neoplasia. |
| Project Title: | Development of semiquantitative multiplex RT-PCR for children cancer profiling using the patented hybridization technology |
| Investigator(s): | Dr. Wong I.H.N., Prof. Tam P.K.H., Dr. Tam J.W.O. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 11/2002 |
| Completion Date: | 10/2003 |
| Abstract: |
| To study cancer-specific mRNA targets involved in tumor progression, it will be selected from the literature and the bioinformatics database for children cancer profiling and differential diagnosis of tumor progression of diverse cancer types. |
| Project Title: | Epigenetic and genetic deregulation in pediatric solid tumors |
| Investigator(s): | Dr. Wong I.H.N., Prof. Tam P.K.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2003 |
| Abstract: |
| To study the transcriptional deregulation of SOCS1, SOCS2 and SOCS3 in pediatric solid tumors and cell lines derived from pediatric cancer patients in vivo; to investigate whether transcriptional repression of SOCS1, SOCS2 and SOCS3 is caused by epigenetic mechanisms, cancer cell lines will be treated with a demethylating drug and an inhibitor of histone deacetylase in order to reactivate their expression; to identify epigenetic and genetic alterations in SOCS1, SOCS2 and SOCS3 associated with the JAK/STAT and RAS signaling pathways in childhood neoplasia. |
| Project Title: | Epigenetic and genetic deregulation in pediatric solid tumors |
| Investigator(s): | Dr. Wong I.H.N., Prof. Tam P.K.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 09/2003 |
| Abstract: |
| To study the transcriptional deregulation of SOCS1, SOCS2 and SOCS3 in pediatric solid tumors and cell lines derived from pediatric cancer patients in vivo; to investigate whether transcriptional repression of SOCS1, SOCS2 and SOCS3 is caused by epigenetic mechanisms, cancer cell lines will be treated with a demethylating drug and an inhibitor of histone deacetylase in order to reactivate their expression; to identify epigenetic and genetic alterations in SOCS1, SOCS2 and SOCS3 associated with the JAK/STAT and RAS signaling pathways in childhood neoplasia. |
| Project Title: | Epigenetic deregulation of imprinted growth/apoptosis regulatory genes in the development of pediatric solid tumors |
| Investigator(s): | Dr. Wong I.H.N., Prof. Tam P.K.H. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 02/2004 |
| Abstract: |
| To study the relationship between epigenetic alterations and transcriptional control of four critical imprinted growth/apoptosis regulatory genes (NOEY2, p73, WT1 and IPL) in pediatric solid tumors and pediatric cancer cell lines; to characterize these four critical imprinted growth/apoptosis regulatory genes with implications for pediatric carcinogenesis in vitro; to restore the normal patterns of regulation and expression of the four critical imprinted growth/apoptosis regulatory genes in pediatric cancer cell lines by demethylation treatment and inhibition of histone deacetylases. |
| List of Research Outputs |
| Hu X., Wong I.H.N. and Chow L.W.C., Tumor-derived aberrant methylation in plasma of invasive ductal breast cancer patients: clinical implications, Oncology Reports. 2003, 10(6): 1811-1815. |
| Wong I.H.N., The sporadic nature of shedding cells in blood: Multiple RNA diagnostic testing and prognostication of cancer progression, Clinical Chemistry. 2003, 49(9): 1429-31. |
| Wong I.H.N., Chan J.K.Y., Wong J.S.W. and Tam P.K.H., Ubiquitous aberrant RASSF1A promoter methylation in childhood neoplasia, 37th Annual Meeting of the Pacific Association of Pediatric Surgeons, Seoul, Korea, 16-20 May, 2004. 43. |
| Researcher : Wong NS |
| Project Title: | Kappa-opioid receptor mediated cellular stress response: the role of Inositol 1,4,5-trisphosphate and diacylglycerol |
| Investigator(s): | Dr. Wong N.S., Dr. Yao K.M. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 10/2002 |
| Abstract: |
| The Phospholipase-C (PLC)/inositol-lipid signaling mechanism is itself of widespread importance in cellular regulation and is coupled to all the three major subtypes of opioid receptors (OR), but how this signaling pathway mediates OR-simulated cellular responses is scarcely known. The objective of this project is to investigate the importance of the PLC/inositol-lipid pathway in KappaOr-induced stress responses. |
| Project Title: | The construction of stable cellular reporter system for the study of the gene expression of Thioredoxia Reductase |
| Investigator(s): | Dr. Wong N.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 10/2002 |
| Completion Date: | 09/2003 |
| Abstract: |
| To produce such stable cellular reporter systems. The availability of these cellular systems is essential for the elucidation of the molecular mechanism by which the expression of the TR-gene is able to cope with the level of extracellular stress. |
| Project Title: | Identification of a novel signaling pathway that regulates the transcription of the stress-response gene GADD153 |
| Investigator(s): | Dr. Wong N.S. |
| Department: | Biochemistry |
| Source(s) of Funding: | Run Run Shaw Research and Teaching Endowment Fund |
| Start Date: | 11/2003 |
| Abstract: |
| To identity the intracellular signaling pathway that is responsible for mediating the effect of fenretinide/4HPR by elucidating the "4HPR-response element" in the proximal promoter of the GADD153 gene. |
| List of Research Outputs |
| Wong N.S., Qiang F., Guo X.X., Yao K.M. and Lee K.S., The Identification of Regulatory Element(s) in the Promoter of the Thioredoxin Reductase gene, ASBMB Annual Meeting and 8th IUBMB conference, June 12-16, 2004, Boston. 2004. |
| Wong N.S., Diao C.T.M. and Wong T.M., The overexpression of Bcl-2 antagonizes the proapoptotic function of the kappa-opioid receptor, Annual New York Academy of Sciences. 2003, 1010: 358-260. |
| Researcher : Wong SYY |
| List of Research Outputs |
| Leung W.L., Wong S.Y.Y., Chan D., Kung H. and Cheah K.S.E., The role/s of type IIA procollagen in BMP (Bone morphogenetic protein) signaling., 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June 2004 Hong Kong.. 2004. |
| Researcher : Wynn SL |
| List of Research Outputs |
| Au Y.K., Wynn S.L., Cheung K.M.C. and Cheah K.S.E., functional analysis of SOX9 by conditional expression of a mutant Sox9, Sox9Y440X, in the notochord, 2004 Sir Edward youde Memorial Fund postgraduate Conference on Model Organism Research and Human Diseases, 14-15 June, 2004 Hong Kong.. 2004. |
| Wynn S.L., Au Y.K. and Cheah K.S.E., Defining the role of Sox9 in early development by tissue specific gene inactivation, The 3rd Australian Developmental Biology Workshop, October 2003, Sydney, Australia. 2003. |
| Researcher : Yao KM |
| Project Title: | FOXM1 and transcriptional regulation of cell cycle progression |
| Investigator(s): | Dr. Yao K.M., Dr. Hui C.C., Dr. Seto E. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2001 |
| Abstract: |
| To study how FOXM1 regulates cyclin expression at the transcriptional level; to study whether FOXM1, like Fkh2, plays a wider role in regulation of cell cycle-dependent gene expression; to study the role of Eps8 as a cooperative factor for FOXM1 function. |
| Project Title: | Investigating the role of a secreted protein sPIN-1 in the regulation of pancreatic b-cell growth and differentiation |
| Investigator(s): | Dr. Yao K.M., Dr. Chung S.K., Dr. Habener J.F., Dr. Thomas M.K. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2002 |
| Abstract: |
| To study: (1) The cellular expression of xPIN-1 in pancreas, (2) The effect of purified sPIN-1 on growth and differentiation of primary and cultured islet cells in vitro; (3) The effect of over-expression sPIN-1 in pancreatic b-cell in transgenic mice. |
| Project Title: | Regulation of FOXM1 activity by the Ras/Raf/MAPK pathway |
| Investigator(s): | Dr. Yao K.M. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 02/2004 |
| Abstract: |
| To perform immunocytochemical, DNA binding and transcriptional analyses to investigate the molecular mechanism(s) underlying the enhancing effect of MEK1 on FOXM1 activity; to enrich our understanding of how the cell cycle is regulated by mitogenic signals, which is of major biological interest and crucial for devising strategies to enhance cell regeneration after tissue injury. |
| List of Research Outputs |
| Wong E.Y.M., Tse J.Y.M., Yao K.M., Lui V.C.H., Tam P.C. and Yeung W.S.B., Identification and characterization of human VCY2-interacting protein: VCY2IP-1, a microtubule-associated protein-like protein, Biology of Reproduction. 2003, 70: 775-784. |
| Researcher : Zhang X |
| List of Research Outputs |
| Zhang X., Copeland N.G., Jenkins N.A. and Huang J., Cell-specific expression of Cre recombinase in Purkinje and retinal bipolar neurons, 43rd American Society for Cell Biology Annual Meeting. Molecular Biology of the Cell (Suppl). 2003, V14: 406a, 2773-2773. |
| Zhang X. and Huang J., Combination of overlapping bacterial artificial chromosomes by a two-step recombinogenic engineering method , Nucleic Acids Research. 2003, 31(15): e81. |
| Zhang X., Ng A.H.L., Tanner J.A., Wu W., Jenkins N.A. and Huang J., Highly Restricted Expression of Cre Recombinase in Cerebellar Purkinje Cells, Genesis: The Journal of Genetics and Development. 2004, V40: 45-51. |
| Zhang X., Thesis title Generation of mouse models to study intracellular transportation in Purkinje cells and Melanocytes, PhD Thesis. 2003. |
| Researcher : Zhou Z |
| Project Title: | Study of perlecan heparan sulfate chains in FGF signaling and angiogenesis |
| Investigator(s): | Dr. Zhou Z.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 01/2003 |
| Completion Date: | 12/2003 |
| Abstract: |
| To investigate whether the loss of Perlecan heparan sulfate chains affects FGF-signaling and angiogenesis; to study the wound healing process and tumor angiogenesis in the mutant mice; to investigate the response of mutant cells to growth factor such as FGFs and EGF; to examine whether lack of Perlecan heparan sulfate affects inflammatory cell infiltration, keratinocyte migration, fibroblast proliferation and angiogenesis; to investigate whether other preteoglycans could compensate to some extent for the loss of Perlecan heparan sulfate. |
| Project Title: | Investigation of alternations in growth factor signaling and their contribution to defective intramembranous bone formation in mice lacking MT1-MMP |
| Investigator(s): | Dr. Zhou Z.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | Hong Kong Research Grants Council Competitive Earmarked Research Grants |
| Start Date: | 09/2003 |
| Abstract: |
| To understand the Nature of defective calvarial osteogenesis and alterations in FGF signaling in MT1-MMP homozygous mutant mice; to regulate the MT1-MMP by FGF signaling. |
| Project Title: | Investigation of alternations in growth factor signaling and their contribution to defective intramembranous bone formation in mice lacking MT1-MMP |
| Investigator(s): | Dr. Zhou Z.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | RGC Projects (Block Grant Funded) |
| Start Date: | 09/2003 |
| Abstract: |
| To understand the Nature of defective calvarial osteogenesis and alterations in FGF signaling in MT1-MMP homozygous mutant mice; to regulate the MT1-MMP by FGF signaling. |
| Project Title: | DNA double-strand break repair and accelerated aging in Zmpste24 deficient mice |
| Investigator(s): | Dr. Zhou Z.J. |
| Department: | Biochemistry |
| Source(s) of Funding: | Block Grant Earmarked for Research |
| Start Date: | 02/2004 |
| Abstract: |
| To look into the double-strand DNA damage repair in Zmpste24 and lamin A mutant cells to understand whether unprocessed prelamin A and lack of lamin A (Knockout mice already got from Dr Stward, NIH) affects homologous recombination and nonhomologous end joining. |
| List of Research Outputs |
| Byrne L.C., Zhou Z., Tryggvason K., Hokfelt T. and Fetissov S., Altered NPY and AgRP in membrane type-1 matrix metalloproteinase-deficient mice, Neuroreport. 2004, 15(3): 569-574. |
| Zhou Z., Doi M. and Wang J., Deletion in laminin a4 results in increased tumor angiogenesis and metastasis, Cancer Research. 2004, 64(12): 4059-4063. |