Dept of Microbiology

THE UNIVERSITY OF HONG KONG
Research and Scholarship 2004

DEPARTMENT OF MICROBIOLOGY

Researcher : Altmeyer RM

List of Research Outputs

Altmeyer R.M., Bartosch B., Virelizier J.L., Arenzana-Seisdedos F., Cosset F.L. and Lozach P.Y., C-type lectins L-SIGN and DC-SIGN capture and transmit infectious hepatitis C virus pseudotype particles , FASEB Journal. 2004, 18 (8):: C31-C31 Suppl. S.

Nal-Rogier B.T.M., Chan C.M., Siu Y.L., Chu S.L. and Altmeyer R.M., Biogenesis and subcellular localization of the SARS coronavirus surface proteins , FASEB JOURNAL. 2004, 18 (8): C71-C71 Suppl.

Researcher : Butt KM

List of Research Outputs

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Researcher : Chan CS

List of Research Outputs

Chu C.M., Leung Y.Y., Hui J.Y., Hung I.F.N., Chan V.L., Leung W.S., Law K.I., Chan C.S., Chan K.S. and Yuen K.Y., Spontaneous pneumomediastinum in patients with severe acute respiratory syndrome, The European respiratory journal. 2004, 23(6): 802-4.

Researcher : Chan KH

List of Research Outputs

Chan C.W., Chiang A.K.S., Chan K.H. and Lau A.S.Y., Epstein-Barr virus-associated infectious mononucleosis in Chinese children, The Pediatric Infectious Disease Journal. USA, Lippincott Williams & Wilkins, Inc, 2003, 22(11): 974-978.

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Cheng V.C.C., Hung I.F.N., Tang B.S., Chu C.M., Wong M.M., Chan K.H., Wu A.K., Wu A.K., Tse D.M., Chan K.S., Zheng B., Peiris J.S.M., Sung J.J. and Yuen K.Y., Viral replication in the nasopharynx is associated with diarrhea in patients with severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(4): 467-75.

Chiu S.S.S., Lau Y.L., Chan K.H., Wong W.H.S. and Peiris J.S.M., The global implications of influenza in Hong Kong, The 11th Asian Congress of Pediatrics & The 1st Asian Congress on Pediatric Nursing, Bangkok, Thailand, 2-7 November 2003.

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Peiris J.S.M., Zheng B., Poon L.L.M., Chan K.H., Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Leung C.S.W., Yuen K.Y. and Leung F.C.C., Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome, The Lancet. 2004, 363: 99-104.

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Peiris J.S.M., Tang W.H., Chan K.H., Khong P.L., Guan Y., Lau Y.L. and Chiu S.S.S., Children with Respiratory Disease associated with Human Metapneumovirus in Hong Kong, Symposium on Legionnaires' Disease and other Respiratory Infections and their Prevention, HK, 12 December 2003.

Peiris J.S.M., Yu W.C., Leung C.W., Cheung C.Y., Ng W.F., Nicholls J.M., Ng T.K., Chan K.H., Lai S.T., Lim W.W.L., Yuen K.Y. and Guan Y., Re-emergence of fatal human influenza A subtype H5N1 disease, Lancet. 2004, 363(9409): 617-619.

Poon L.L.M., Chan K.H., Nicholls J.M., Zheng B., Yuen K.Y., Guan Y. and Peiris J.S.M., Characterization of a novel coronavirus responsible for severe acute respiratory syndrome, Options for the Control of Influenza V. 2004, International Congress Series 1263: 805-808.

Poon L.L.M., Chan K.H. and Peiris J.S.M., Crouching tiger, hidden gragon: the laboratory diagnosis of severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(2): 297-9.

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Poon L.L.M., Chan K.H., Wong O.K., Yam W.C., Yuen K.Y., Guan Y., Lo Y.M. .D.E.N.N.I.S. and Peiris J.S.M., Early diagnosis of SARS Coronavirus infection by real time RT-PCR, Journal of Clinical Virology. 2003, 28(3): 233-8.

Poon L.L.M., Leung C.S., Tashiro M., Chan K.H., Wong B.W., Yuen K.Y., Guan Y. and Peiris J.S.M., Rapid Detection of the Severe Acute Respiratory Syndrome (SARS) Coronavirus by a Loop-Mediated Isothermal Amplification Assay, Clinical Chemistry . 2004, 50: 1050-52.

Poon L.L.M., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S.M. and Guan Y., Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). , Clin Chem. 2003, 49: 953-5.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Chan K.H., Tam V.K., Peiris J.S.M. and Yuen K.Y., Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnsosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2004, 42(5): 2306-9.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Yam W.C., Chan K.H., Poon L.L.M., Guan Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus, Journal of Clinical Microbiology. 2003, 41(10): 4521-4524.

Zeng F., Chow Y.C.K., Hon C.C., Law K.M., Yip C.W., Chan K.H., Peiris J.S.M. and Leung F.C.C., Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments, Biochemical and Biophysical Research Communications. 2004, 315: 1134-1139.

Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Wang Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., The Complete Genome Sequence of Severe Acute Respiratory Syndrome Coronavirus Strain HKU-39849 (HK-39), Experimental Biology Medicine. 2003, 228: 866-873.

Zhong N.S., Zheng B., Li Y.M., Poon L.L.M., Xie Z.H., Chan K.H., Li P.H., Li P.H., Li P.H., Tan S.Y., Chang Q., Xie J.P., Liu X.Q., Xu J., Li D.X., Yuen K.Y., Peiris J.S.M. and Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003, The Lancet. 2003, 362(9393): 1353-8.

Researcher : Chan KM (Chan King Man)

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Woo G.K.S., Fung A.M.Y., Wong M.K.M., Chan K.M., Tam D.M.W. and Yuen K.Y., Eggerthella hongkongensis sp. nov. and Eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia, Diagnostic Microbiology and Infectious Disease. Elsevier, 2004, 49: 255-263.

Researcher : Chan KM (Chan Kit Man)

List of Research Outputs

Woo P.C.Y., Tse H., Chan K.M., Lau S.K.P., Fung A.M., Yip K.T., Tam D.M., Ng K.H., Que T.L. and Yuen K.Y., Streptococcus milleri" endocarditis caused by Streptococcus anginosus, Diagnostic Microbiology and Infectious Disease . 2004, 48: 81-8.

Researcher : Chan KS

List of Research Outputs

Chu C.M., Leung Y.Y., Hui J.Y., Hung I.F.N., Chan V.L., Leung W.S., Law K.I., Chan C.S., Chan K.S. and Yuen K.Y., Spontaneous pneumomediastinum in patients with severe acute respiratory syndrome, The European respiratory journal. 2004, 23(6): 802-4.

Researcher : Chen H

List of Research Outputs

Huang J., Chen H., Hutt-Fletcher L., Ambinder R.F. and Hayward S.D., Lytic viral replication as a contributor to the detection of Epstein-Barr virus in breast cancer, Journal of Virology . 2003, 77: 13267-74.

Wu F.Y., Wang S.E., Chen H., Wang L., Hayward S.D. and Hayward G.S., CCAAT/enhancer binding protein alpha binds to the Epstein-Barr virus(EBV) ZTA protein through oligomeric interactions and contributes to cooperative transcriptional activation of the ZTA promoter through direct binding to the ZII and ZIII motifs during induction of the EBV lytic cycle, Journal of Virology . 2004, 78: 4847-65.

Researcher : Cheng SLE

List of Research Outputs

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distrubtion and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong Children, Vaccine. Elsevier Ltd, 2004, 22: 3334-3339.

Researcher : Cheng VCC

List of Research Outputs

Au W.Y., Cheng V.C.C., Ho P.L., Yuen K.Y., Hung I., Ma S.Y., Lie A.K.W., Liang R.H.S. and Kwong Y.L., Non tuberculous mycobacterial infections in Chinese hematopoietic stem cell transplantation recipients., Bone Marrow Transplantation. 2003, 32: 709-714.

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Che X.Y., Qiu L.W., Pan Y.X., Wen K., Hao W., Zhang L.Y., Wang Y.D., Liao Z.Y., Hua X., Cheng V.C.C. and Yuen K.Y., Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome. , Journal of Clinical Microbiology. 2004, 42(6): 2629-35.

Cheng V.C.C., Yam W.C., Hung I.F., Woo P.C.Y., Lau S.K.P., Tang S.F. and Yuen K.Y., Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis, Journal of Clinical Pathology . 2004, 57: 281-5.

Cheng V.C.C., Hung I.F.N., Wu A.K., Tang S.F., Chu C.M. and Yuen K.Y., Lymphocyte surge as a marker for immunorestitution disease due to Pneumocystis jiroveci pneumonia in HIV-negative immunosuppressed hosts., European journal of clinical microbiology & infectious diseases. 2004, 23(6): 512-4.

Cheng V.C.C., Yam W.C., Woo P.C.Y., Lau S.K.P., Hung I.F., Wong S.P., Cheung W.C. and Yuen K.Y., Risk Factors for Development of Paradoxical Response During Antituberculosis Therapy in HIV-Negative Patients, Eur J Clin Microbiol Infect Dis. 2003, 22: 597-602.

Cheng V.C.C., Hung I.F.N., Tang B.S., Chu C.M., Wong M.M., Chan K.H., Wu A.K., Wu A.K., Tse D.M., Chan K.S., Zheng B., Peiris J.S.M., Sung J.J. and Yuen K.Y., Viral replication in the nasopharynx is associated with diarrhea in patients with severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(4): 467-75.

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Ma S.Y., Lau G., Cheng V.C.C. and Liang R.H.S., Hepatitis B reactivation in patients positive for hepatitis B surface antigen undergoing autologous hematopoietic cell transplantation, Leukemia and Lymphoma. 2003, 44(8): 1281-5.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Yam W.C., Cheng V.C.C., Hui W.T., Wang L.N., Seto W.H. and Yuen K.Y., Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA), Diagnostic Microbiology and Infectious Disease . 2004, 48: 271-5.

Researcher : Cheung CL

List of Research Outputs

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Researcher : Cheung CY

List of Research Outputs

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Lee D.C.W., Li C.B., Law H.Y., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., A Tale of Two Viruses: Cytokine Dysregulation in HIV and H5N1 Infections, 3^rd International Proteomics Conference (IPC'03), 1^st Taiwan Proteomics Conference, Joint Meeting of AOHUPO 2004, Taipei, Taiwan, 14-17 May 2004.

Peiris J.S.M., Yu W.C., Leung C.W., Cheung C.Y., Ng W.F., Nicholls J.M., Ng T.K., Chan K.H., Lai S.T., Lim W.W.L., Yuen K.Y. and Guan Y., Re-emergence of fatal human influenza A subtype H5N1 disease, Lancet. 2004, 363(9409): 617-619.

Researcher : Cheung CY

List of Research Outputs

Researcher : Cheung KW

List of Research Outputs

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Researcher : Chow KH (Chow Kin Hung)

List of Research Outputs

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distrubtion and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong Children, Vaccine. Elsevier Ltd, 2004, 22: 3334-3339.

Researcher : Chow KH (Chow Ka Hang)

List of Research Outputs

Ho P.L., Wong R.C., Chow K.H., Cheung M.Y., Wong S.S.Y., Yam W.C. and Que T.L., Application of a multiplex pbp2b and pbp2x PCR for prediction of penicillin resistance in Streptococcus pneumoniae., Journal of Antimicrobial Chemotherapy. 2004, 53(5): 890-1.

Xing B., Ho P.L., Yu C.W., Chow K.H., Gu H. and Xu B., Self-assembled multivalent vancomycin on cell surfaces against vancomycin-resistant enterococci (VRE) , Chemical Communications. 2003, 17: 2224-5.

Researcher : Guan Y

Project Title:	Molecular evolution studies on H5N1/97 and related avian influenza viruses
Investigator(s):	Dr. Guan Y., Prof. Peiris J.S.M., Prof. Shortridge K.F.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2001

Abstract:

To develop methods that can be applied to screen large numbers of virus isolates for genetic reassortants; to understand how the precursors of H5N1/97 virus evolved, and continue to evolve, in their hosts in southern China.

Project Title:	Vaccines for the control of H5N1 and other avian influenza viruses
Investigator(s):	Dr. Guan Y.
Department:	Microbiology
Source(s) of Funding:	HKU Foundation Seed Grant
Start Date:	04/2002

Abstract:

To study vaccines for the control of H5N1 and other avian influenza viruses.

Project Title:	Genetic determinants of influenza virus for inter-species transmission: studies using a reverse genetic approach
Investigator(s):	Dr. Guan Y., Prof. Peiris J.S.M., Dr. Poon L.L.M., Prof. Shortridge K.F.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	08/2002

Abstract:

Avian influenza viruses can directly impact on human and animal health and thus cause economic loss. The study aims to establishing a reverse genetic system and to determine the biological significance of the deletion of NA stalk and the NS genes, particularly in relation to the host-restriction and pathogenesis. Results can enhance understanding of the machanism of inter-species transmission of influenza virus.

Project Title:	Searching for the precursor of SARS-Cov in Southern China
Investigator(s):	Dr. Guan Y.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To find source of SARS-Cov, and how this virus emerged in humans.

Project Title:	The role of migratory birds for evolution of influenza gene pool of southern China
Investigator(s):	Dr. Guan Y., Prof. Li K.S., Dr. Poon L.L.M.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To observe the epidemiological dynamics of influenza viruses between domestic ducks (the major influenza gene reservoirs in this region) and migratory birds in southern China; to understand the mechanisms of inter-species transmission of influenza virus from wild aquatic birds to domestic poultry, or vice versa; to determine whether migratory birds may contribute some gene segments for the generation of influenza viruses of pandemic potential.

List of Research Outputs

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Guan Y., Peiris J.S.M., Zheng B., Poon L.L.M., Chan K.H., Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Leung C.S.W., Yuen K.Y. and Leung F.C.C., Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome, The Lancet. 2004, 363: 99-104.

Guan Y., Peiris J.S.M., Poon L.L.M., Dyrting K.C., Ellis T.M., Sims L., Webster R.G. and Shortridge K.F., Reassortants of H5N1 influenza viruses recently isolated from aquatic poultry in Hong Kong SAR., Avian Diseases. 911-3, 2003, 47(3 Suppl).

He M.L., Zheng B., Peng Y., Peiris J.S., Poon L.L., Yuen K.Y., Lin M.C., Kung H. and Guan Y., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, Journal of the American Medical Association. 2003, 290(20): 2665-6.

Horinoto T., Fukuda N., Iwatsuki-Horimoto K., Guan Y., Lim W.W.L., Peiris J.S.M., Sugii S., Odagiri T., Tashiro M. and Kawaoka Y., Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003, Journal of Veterinary Medical Science. 2004, 66(3): 303-5.

Kung N.Y., Guan Y., Perkins N.R., Bissett L., Ellis T., Sims L., Morris R.S., Shortridge K.F. and Peiris J.S.M., The impact of a monthly rest day on avian influenza virus isolation rates in retail live poultry markets in Hong Kong., Avian Diseases. 2003, 47(3 Suppl): 1037-41.

Liu M., Guan Y., Peiris J.S.M., He S., Webby R.J., Perez D. and Webster R.G., The quest of influenza A viruses for new hosts, Avian Diseases. 2003, 47(3 Suppl): 849-56.

Peiris J.S.M., Tang W.H., Chan K.H., Khong P.L., Guan Y., Lau Y.L. and Chiu S.S.S., Children with Respiratory Disease associated with Human Metapneumovirus in Hong Kong, Symposium on Legionnaires' Disease and other Respiratory Infections and their Prevention, HK, 12 December 2003.

Peiris J.S.M., Yu W.C., Leung C.W., Cheung C.Y., Ng W.F., Nicholls J.M., Ng T.K., Chan K.H., Lai S.T., Lim W.W.L., Yuen K.Y. and Guan Y., Re-emergence of fatal human influenza A subtype H5N1 disease, Lancet. 2004, 363(9409): 617-619.

Poon L.L.M., Chan K.H., Nicholls J.M., Zheng B., Yuen K.Y., Guan Y. and Peiris J.S.M., Characterization of a novel coronavirus responsible for severe acute respiratory syndrome, Options for the Control of Influenza V. 2004, International Congress Series 1263: 805-808.

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Poon L.L.M., Chan K.H., Wong O.K., Yam W.C., Yuen K.Y., Guan Y., Lo Y.M. .D.E.N.N.I.S. and Peiris J.S.M., Early diagnosis of SARS Coronavirus infection by real time RT-PCR, Journal of Clinical Virology. 2003, 28(3): 233-8.

Poon L.L.M., Leung C.S., Tashiro M., Chan K.H., Wong B.W., Yuen K.Y., Guan Y. and Peiris J.S.M., Rapid Detection of the Severe Acute Respiratory Syndrome (SARS) Coronavirus by a Loop-Mediated Isothermal Amplification Assay, Clinical Chemistry . 2004, 50: 1050-52.

Poon L.L.M., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S.M. and Guan Y., Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). , Clin Chem. 2003, 49: 953-5.

Sims L.D., Guan Y., Ellis T.M., Liu K.K., Dyrting K., Wong H., Kung N.Y., Shortridge K.F. and Peiris J.S.M., An update on avian influenza in Hong Kong 2002., Avian Diseases. 2003, 47(3 Suppl): 1083-6.

Sims L.D., Ellis T.M., Liu K.K., Dyrting K., Wong H., Peiris J.S.M., Guan Y. and Shortridge K.F., Avian Influenza in Hong Kong 1997-2002., Avian Diseases. 2003, 47(3 Suppl): 832-838.

Snijder E.J., Bredenbeek P.J., Dobbe J.C., Thiel V., Ziebuhr J., Poon L.L.M., Guan Y., Rozanov M., Spaan W.J. and Gorbalenya A.E., Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage , Journal of Molecular Biology. 2003, 331(5): 991-1004.

Sturm-Ramirez K.M., Ellis T., Bousfield B., Guan Y., Peiris J.S.M. and Webster R.G., H5N1 influenza A viruses from 2002 are highly pathogenic in waterfowl, Options for the Control of Influenza V. 2004, International Congress Series 1263: 200-204.

Sturm-Ramirez K.M., Ellis T., Bousfield B., Bissett L., Dyrting K., Rehg J.E., Poon L.L.M., Guan Y., Peiris J.S.M. and Webster R.G., Reemerging H5N1 influenza viruses in Hong Kong in 2002 are highly pathogenic to ducks., Journal of Virology. 2004, 78(9): 4892-901.

Webby R.J., Perez D.R., Coleman J.S., Guan Y., Knight J.H., Govorkova E.A., McClain-Moss L.R., Peiris J.S.M., Rehg J.E., Tuomanen E.I. and Webster R.G., Responsiveness to a pandemic alert: use of reverse genetics for rapid development of influenza vaccines., The Lancet. 2004, 363(9415): 1099-103.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Yam W.C., Chan K.H., Poon L.L.M., Guan Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus, Journal of Clinical Microbiology. 2003, 41(10): 4521-4524.

Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Wang Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., The Complete Genome Sequence of Severe Acute Respiratory Syndrome Coronavirus Strain HKU-39849 (HK-39), Experimental Biology Medicine. 2003, 228: 866-873.

Zheng B., Guan Y., Tang Q., Cheng D., Xie F.Y., He M.L., Chan K.W., Wong K.L., Lader E., Woodle M.C., Lu P.Y., Li B. and Zhong N., Prophylactic and therapeutic effects of small interfering RNA targeting SARS-coronavirus, Antiviral Therapy. 2004, 9: 365-374.

Zheng B., Guan Y., Wong K.H., Zhou J., Wong K.L., Young B.W.Y., Lu L. and Lee S.S., SARS-related virus predating SARS outbreak, Hong Kong, Emerging Infectious Diseases. 2004, 10(2): 176-178.

Zhong N.S., Zheng B., Li Y.M., Poon L.L.M., Xie Z.H., Chan K.H., Li P.H., Li P.H., Li P.H., Tan S.Y., Chang Q., Xie J.P., Liu X.Q., Xu J., Li D.X., Yuen K.Y., Peiris J.S.M. and Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003, The Lancet. 2003, 362(9393): 1353-8.

Researcher : Ho PL

Project Title:	A phenotypic and genotypic analysis of vancomycin tolerance in Streptococcus pnemoniae
Investigator(s):	Dr. Ho P.L., Dr. Que T.L., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2000

Abstract:

To identify the frequency of occurrence of vancomycin tolerance in Streptococcus pneumoniae; to identify novel mutations in the histdine kinase (vncS) of tolerant pneumococci.

Project Title:	Molecular epidemiology of fluoroquinolone-resistant streptococcus pneumoniae in Hong kong and Canada
Investigator(s):	Dr. Ho P.L., Dr. Low D.E.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001

Abstract:

To define the molecular epidemiology of fluoroquinolone-resistant strains of S. pneumoniae isolated in Hong Kong and Canada.

Project Title:	Clinical outcome and impact on health care utilization of invasive pneumococcal disease in the era of antimicrobial resistance
Investigator(s):	Dr. Ho P.L., Dr. Cheng Y.H., Dr. Chiu S.S.S., Dr. Ng T.K., Dr. Que T.L., Dr. Tsang D.N.C., Dr. Tsang K.W.T., Dr. Yung R.W.H.
Department:	Microbiology
Source(s) of Funding:	Health Care and Promotion Fund
Start Date:	08/2002

Abstract:

To determine the impact of antibiotic resistance on patient outcome in invasive pneumococcal disease and to what extent additional health care resources are utilized as a result of the antibiotic resistance in a cohort of patients between 1997 to 2000; to document the serotypes of S. pneumoniae that casue invasive infection in the same cohort of patients.

Project Title:	Relation between antibiotic resistance in Escherichia coli of human and poultry origins
Investigator(s):	Dr. Ho P.L., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To examine the relationship of antibiotic-resistant Escherichia coli isolated from poultry, hospital patients, and normal people.

Project Title:	Risk factors for SARS in health care workers following incubation of SARS patients
Investigator(s):	Dr. Ho P.L., Dr. Ho E., Dr. Hung C.T., Dr. Law K.I., Dr. Yap F.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To identify factors associated with SARS transmission in HCW who participated in intubation of SARS patients; to define the transmission probability of an intubation procedure of SARS patient and to describe the intubation procedural practice of clinicians in the first two months of the outbreak.

Project Title:	Fluoroquinolone susceptibility clinical breakpoints and fluoroquinolone-resistant determining mutations among clinical strains of streptococcus pneumoniae
Investigator(s):	Dr. Ho P.L., Dr. Wong S.S.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To determine the frequency of occurrence of first step fluoroquinolone-resistant determining mutations among isolates categorized as susceptible by the currently accepted clinical breakpoints.

List of Research Outputs

Au W.Y., Cheng V.C.C., Ho P.L., Yuen K.Y., Hung I., Ma S.Y., Lie A.K.W., Liang R.H.S. and Kwong Y.L., Non tuberculous mycobacterial infections in Chinese hematopoietic stem cell transplantation recipients., Bone Marrow Transplantation. 2003, 32: 709-714.

Chan K.H., Ho P.L., Cheung R.T.F., Tsang K.L., Fong G.C.Y., Cheng P.W. and Ho S.L., Tuberculous meningitis with tuberculomata presenting as postpartum pyrexia of unknown origin. , Hospital Medicine. 2003, 64: 306-307.

Chan M.M.W., Ooi C.G.C., Hui D.S., Ho P.L. and Tsang K.W.T., Severe acute respiratory syndrome., International Journal of Tuberculosis and Lung Disease. 2003, 7(12): 1117-30.

Chung B.H.Y., Chan G.C.F., Ho P.L., Ha S.Y. and Lau Y.L., Bacillus sp. sepsis in pediatric oncology patients with neutropenic fever, Joint Annual Scientific Meeting 2003 of the Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association Limited, QEH, Hong Kong, 13 December 2003. 82.

Gu H., Ho P.L., Tong F.H., Xu C. and Xu B., Presenting vancomycin on nanoparticles to enhance antimicrobial activities, Nano Letters. 2003, 3(9): 1261-3.

Gu H., Ho P.L., Tsang K.W., Wang L. and Xu B., Using biofunctional magnetic manoparticles to capture vancomycin-resistant enterococci and other gram-positive bacteria at ultralow concentration, Journal of American Chemical Society. 2003, 125(51): 15702-3.

Gu H., Ho P.L., Tsang K.W.T., Yu C.W. and Xu B., Using biofunctional magnetic nanoparticles to capture gram-negative bacteria at an ultra-low concentration , Chemical Communications. 2003, 15: 1966-7.

Ho J.C.M., Wong R.W.M., Ooi C.G.C., Mok T.Y., Hung I.F., Ng W., Lam Y.M., Tam W.O., Wong P.C., Ho P.L., Lai C.L., Lam W.K., Lam S.K. and Tsang K.W.T., Correlation of Liver Dysfunction And Disease Activity In Patients With Severe Acute Respiratory Syndrome, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-08.

Ho J.C.M., Ooi C.G.C., Mok T.Y., Chan J.W., Hung I., Lam B., Wong P.C., Li P.C., Ho P.L., Lam W.K., Ng C.K., Ip M.S.M., Lai K.N., Chan M.M.W. and Tsang K.W.T., High-dose pulse versus nonpulse corticosteroid regimens in severe acute respiratory syndrome, American Journal of Respiratory and Critical Care Medicine. 2003, 168: 1449-1456.

Ho J.C.M., Ooi C.G.C., Mok T.Y.M., Ho P.L., Chan M.M.W., Ip M.S.M., Lam W.K. and Tsang K.W.T., Initial pulse or non-pulse steroid therapy in patients with deteriorating severe acute respiratory syndrome, 8th Asian Pacific Society of Respirology Congress, Kuala Lumpur, Malaysia,2003. Abstract book PP6-14: p.193.

Ho J.C.M., Wu A.Y.Y., Ooi C.G.C., Lam B., Ho P.L., Chan M.M.W., Ip M.S.M., Lam W.K. and Tsang K.W.T., Pentaglobin as rescue therapy in patients with severe acute respiratory syndrome, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-04.

Ho J.C.M., Ooi C.G.C., Mok T.Y., Ho P.L., Chan M.M.W., Ip M.S.M., Lam W.K. and Tsang K.W.T., Pulse corticosteroid therapy in patients with severe acute respiratory syndrome, 9th Medical Research Conference, Medical Science Group, The University of Hong Kong. 2004, Abstract Book: RC-05.

Ho P.L., Wong R.C., Chow K.H., Cheung M.Y., Wong S.S.Y., Yam W.C. and Que T.L., Application of a multiplex pbp2b and pbp2x PCR for prediction of penicillin resistance in Streptococcus pneumoniae., Journal of Antimicrobial Chemotherapy. 2004, 53(5): 890-1.

Ho P.L., Tang X.P. and Seto W.H., SARS: hospital infection control and admission strategies., Respirology. 2003, Suppl: S41-5.

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distrubtion and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong Children, Vaccine. Elsevier Ltd, 2004, 22: 3334-3339.

Lam M.F., Ooi C.G.C., Lam B., Ho J.C.M., Seto W.H., Ho P.L., Wong P.C., Liang R.H.S., Lam W.K. and Tsang K.W.T., An indolent case of severe acute respiratory syndrome, American Journal of Respiratory and Critical Care Medicine. 2004, 169(1): 125-128.

Lee C.K., Ho P.L., Lee K.Y., Cheng W.W., Chan N.K., Tsoi W.C. and Lin C.K., Estimation of bacterial risk in extending the shelf life of PLT concentrates from 5 to 7 days. , Transfusion. 2003, 43(8): 1047-52.

Leung E.T.Y., Kam K.M., Chiu A., Ho P.L., Seto W.H., Yuen K.Y. and Yam W.C., Detection of katG Ser315Thr substitution in respiratory specimens from patients with isoniazid-resistant Mycobacterium tuberculosis using PCR-RFLP, Journal of Medical Microbiology. 2003, 52(Pt 11): 999-1003.

Ooi C.G.C., Khong P.L., Lam B., Ho J.C.M., Yiu W.C., Wong R.W.M., Wang T., Ho P.L., Wong P.C., Chan R.H., Lam W.K., Lai K.N. and Tsang K.W.T., Relationship between radiological and clinical parameters in SARS, 8th Asian Pacific Society of Respirology Congress, Kuala Lumpur, Malaysia, 2003. Abstract Book: OP2-01, p.73.

Ooi C.G.C., Khong P.L., Lam B., Ho J.C.M., Yiu W.C., Wong R.W.M., Wang T., Ho P.L., Chan R.H., Lam W.K., Lai K.N. and Tsang K.W.T., Severe acute respiratory syndrome : Relationship between radiological and clinical parameters, Radiology. 2003, 229: 492-499.

Tsang K.W.T., Tipoe G.L., Sun J.Z., Mak J.C.W., Leung Y.H., Yan C.P.K., Ko K.W., Ooi C.G.C., Ho P.L., Ho J.C.M. and Lam W.K., Central microtubular ciliary defects are of clinical significance in bronchiectasis, 8th Asian Pacific Society of Respirology Congress, Kuala Lumpur, Malaysia. 2003, Abstract Book PP6-20: p.199.

Tsang K.W.T., Tipoe G.L., Sun J.Z., Mak J.C.W., Leung Y.H., Yan C.P.K., Ko K.W., Ooi C.G.C., Ho P.L., Ho J.C.M. and Lam W.K., Ciliary central microtubular orientation is of no clinical significance in bronchiectasis, 8th Asian Pacific Society of Respirology Congress, Kuala Lumpur, Malaysia. 2003, Abstract Book PP6-21: p.200.

Tsang K.W.T., Ho P.L., Tan K.C., Ooi C.G.C., Ho J.C.M., Tipoe G.L., Lam W.K. and Chan M.M.W., Efficacy of 12-month treatment with inhaled corticosteroid in bronchiectasis, European Respiratory Journal. 2003, suppl 22(45): P3213.

Tsang K.W.T., Tipoe G.L., Sun J.Z., Leung Y.H., Ho P.L., Yuen K.Y., Mak J.C.W. and Lam W.K., Helicobacter pylori exotoxin disrupts ciliary beating and ultrastructure of human airway mucosa, 8th Asian Pacific Society of Respirology Congress, Kuala Lumpur, Malaysia. 2003, Abstract Book PP6-22: p.201.

Wong S.S.Y., Woo P.C.Y., Ho P.L. and Wang T.K.F., Continuous ambulatory peritoneal dialysis-related peritonitis caused by Streptococcus bovis. , Eur J Clin Microbiol Infect Dis.. 2003, 22(7): 424-6.

Wong W.M., Ho J.C.M., Hung I.F., Ng W., Lam Y.M., Tam W.O., Wong B.C.Y., Wong P.C., Lai C.L., Lam W.K., Lam S.K., Tsang K.W.T., Ooi C.G.C., Ho P.L., Mok T. and Chan J., Temporal Patterns of Hepatic Dysfunction and Disease Severity in Patients with SARS, Journal of the American Medical Association Southeast Asia (JAMA). 2003, 290(20): 2663-2665.

Wu A.Y.Y., Ooi C.G.C., Lam B., Mok T.Y.M., Ho P.L., Chan M.M.W., Ip M.S.M., Lam W.K. and Tsang K.W.T., The use of pentaglobin in patients with deteriorating severe acute respiratory syndrome, 8th Asian Pacific Society of Respirology Congress, Kuala Lumpur, Malaysia . 2003, Abstract Book PP6-15: p.194.

Xing B., Ho P.L., Yu C.W., Chow K.H., Gu H. and Xu B., Self-assembled multivalent vancomycin on cell surfaces against vancomycin-resistant enterococci (VRE) , Chemical Communications. 2003, 17: 2224-5.

Researcher : Huang Y

List of Research Outputs

Chong K.T., Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., AFMP2 Encodes a Novel Immunogenic Protein of the Antigenic Mannoprotein Superfamily in Aspergillus fumigatus. , Journal of Clinical Microbiology . 2004, 42: 2287-91.

Researcher : Hung IFN

List of Research Outputs

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Cheng V.C.C., Hung I.F.N., Wu A.K., Tang S.F., Chu C.M. and Yuen K.Y., Lymphocyte surge as a marker for immunorestitution disease due to Pneumocystis jiroveci pneumonia in HIV-negative immunosuppressed hosts., European journal of clinical microbiology & infectious diseases. 2004, 23(6): 512-4.

Cheng V.C.C., Hung I.F.N., Tang B.S., Chu C.M., Wong M.M., Chan K.H., Wu A.K., Wu A.K., Tse D.M., Chan K.S., Zheng B., Peiris J.S.M., Sung J.J. and Yuen K.Y., Viral replication in the nasopharynx is associated with diarrhea in patients with severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(4): 467-75.

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Chu C.M., Leung Y.Y., Hui J.Y., Hung I.F.N., Chan V.L., Leung W.S., Law K.I., Chan C.S., Chan K.S. and Yuen K.Y., Spontaneous pneumomediastinum in patients with severe acute respiratory syndrome, The European respiratory journal. 2004, 23(6): 802-4.

Researcher : Im SWK

Project Title:	Investigation of community exposure to a novel coronavirus associated with SARS and herd immunity developed among healthy blood donors
Investigator(s):	Dr. Im S.W.K., Dr. Lee C.K., Dr. Yam W.C.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To determine the exposure of the Hong Kong community to the novel coronavirus and the extent of herd immunity developed; to optimize a cost-effective protocol suitable for high throughput blood testing screening.

Project Title:	Dendritic cell based immune intervention of nasopharyngeal carcinoma
Investigator(s):	Dr. Im S.W.K., Dr. Chua D.T.T., Dr. Zheng B.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To conduct an effective, individual EBV antigen expression system which will allow us to obtain large amount of such antigens; to detect if the DCs from NPC patients are selectively functionally defective to individual EBV antigens, and if so, whether cytokine-activate DCs may overcome the deficiency; to ascertain whether the cytokine-activated DCs of NPC patients plus the antigens can ex vivo activate specific CTL precursors or stimulate naive T cells to become specific CTLs, including optimization of the conditions for expansion of these specific (CTL cell lines in vitro; to evaluate whether the activated DCs together with the EBV antigens may indeed stimulate the target specific CTLs in NPC patients after removal of the cancer by traditional therapy.

Researcher : Kao RYT

Project Title:	Staphylococcal methionine aminopeptidase: a potential drug target for antimicrobial therapeutics
Investigator(s):	Dr. Kao R.Y.T., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	01/2003
Completion Date:	12/2003

Abstract:

The vitality of the map gene in Gram positive bacteria, such as staphylococcus, streptococcus, and bacillus, however, is still elusive. Demonstration experimentally of the essentiality of map gene in Gram positive bacteria will subsequently validate methionine aminopeptiase as an effective antimicrobial target against a variety of pathogens, from fungi to bacteria.

Project Title:	The application of high-throughput screening (HTS) technologies to identify inhibitors of SARS-associated coronavirus main proteinase
Investigator(s):	Dr. Kao R.Y.T., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To explore technologies for identifying inhibitors of SARS-associated coronavirus main proteinase.

Project Title:	Application of chemical genetic approaches to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei
Investigator(s):	Dr. Kao R.Y.T., Dr. Madar J.C.S., Dr. Sun H.Z., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2003

Abstract:

In conjunction with conventional genetic approaches (such as gene cloning and gene inactivation, etc.), we set out to employ chemical genetic approach - the use of high-throughput screening (HTS) technologies to identify biologically active small molecules that will interfere with particular cellular processes/biological pathways/gene products - to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei. Forward chemical genetics will be used to identify small molecule compounds that will interfere with host-pathogen interactions and dimorphism in P. marneffei. Reverse chemical genetics will be used to identify small molecules that will have specific inhibitory effects on MetAP1 or MetAP2 (essential enzymes involved in removal of N-terminal methionine) of P. marneffei. Resulting specific inhibitors of MetAPs will be tested in in vitro intracellular survival and in vivo animal infection models.

Project Title:	Application of chemical genetic approaches to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei
Investigator(s):	Dr. Kao R.Y.T., Dr. Madar J.C.S., Dr. Sun H.Z., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	12/2003

Abstract:

Project Title:	Cloning of SARS-associated coronavirus main proteinase (SARS-CoV M^pro and identification of SARS-CoVM^pro specific synthetic substrates
Investigator(s):	Dr. Kao R.Y.T., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	02/2004

Abstract:

To develop a fluorescence-based continuous enzymatic assay for the rapid evaluation of potential inhibitors of SARS-CoV M^pro; so as to lay down the foundation for researches in inhibitors of various 3C-like viral proteases.

List of Research Outputs

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Researcher : Kung NY

List of Research Outputs

Kung N.Y., Guan Y., Perkins N.R., Bissett L., Ellis T., Sims L., Morris R.S., Shortridge K.F. and Peiris J.S.M., The impact of a monthly rest day on avian influenza virus isolation rates in retail live poultry markets in Hong Kong., Avian Diseases. 2003, 47(3 Suppl): 1037-41.

Sims L.D., Guan Y., Ellis T.M., Liu K.K., Dyrting K., Wong H., Kung N.Y., Shortridge K.F. and Peiris J.S.M., An update on avian influenza in Hong Kong 2002., Avian Diseases. 2003, 47(3 Suppl): 1083-6.

Researcher : Lai WM

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Researcher : Lau SKP

Project Title:	Molecular characterization of group G b-hemolytic streptococcus isolated from bacteremic patients and analysis of prognostic factors
Investigator(s):	Dr. Lau S.K.P., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	12/2001
Completion Date:	11/2003

Abstract:

To compare the usefulness of the Vitek System (GPI), the API system (20 STREP), and the ATB expression system (ID32 STREP) in seciating the isolates with gold standard; to analyze the epidemiology, clinical diseases, and outcome of patients that developed group G b-hemolytic streptococcal bacteremia.

Project Title:	Molecular characterization of anaerobic Gram-positive non-sporulating rods and its clinical significance
Investigator(s):	Dr. Lau S.K.P., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2002

Abstract:

The study proposes to use 16S ribosomal RNA gene sequencing as a way of genotypic identification for all clinical isolates of this group of bacteria failed to be identified by conventional methods. Using this approach, the research team aims at guiding clinicians to more appropriate treatment regimen and a better characterization of the clinical spectrum of diseases.

Project Title:	Molecular characterization of Streptococcus iniae isolated from patients outside North America
Investigator(s):	Dr. Lau S.K.P., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To define the 2 isolates by 16S ribosomal RNA gene sequencing and perform PEGE on these 2 isolates, together with a type strain and the disease-associated strain from Canada.

Project Title:	Detection of nucleocapsid protein in patients with SARS related coronavirus infections
Investigator(s):	Dr. Lau S.K.P., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To develop an enzyme-linked immunosorbant assay (ELISA)-based antigen test for rapid diagnosis of Severe Acute Respiratory Syndrome (SARS).

Project Title:	Enzyme-linked immunosorbant assay (ELISA) - based antigen test for rapid diagnosis of severe acute respiratory syndrome (SARS)
Investigator(s):	Dr. Lau S.K.P., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	07/2003
Completion Date:	06/2004

Abstract:

To develop an enzyme-linked immunosorbant assay (ELISA)-based antigen test for diagnosis of Severe Acute Respiratory Syndrome (SARS); to define the role of the antigen test in the rapid diagnosis of SARS in clinical studies; to determine the patterns of viral shedding in patients with SARS.

Project Title:	Molecular characterization and pathogenesis of a rare bacterium causing psoas abscess
Investigator(s):	Dr. Lau S.K.P., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To characterize the pathogenesis of a rare bacterium associated wth psoas abscess by sequencing its 16S rRNA gene, M protein gene (emm) and streptolysin S structural gene (sagA); to characterize the bacterium's clonality by mulit-locus sequence typing, thus allowing an estimation on the potential of similar diseases in other parts of the world.

List of Research Outputs

Cheng V.C.C., Yam W.C., Hung I.F., Woo P.C.Y., Lau S.K.P., Tang S.F. and Yuen K.Y., Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis, Journal of Clinical Pathology . 2004, 57: 281-5.

Cheng V.C.C., Yam W.C., Woo P.C.Y., Lau S.K.P., Hung I.F., Wong S.P., Cheung W.C. and Yuen K.Y., Risk Factors for Development of Paradoxical Response During Antituberculosis Therapy in HIV-Negative Patients, Eur J Clin Microbiol Infect Dis. 2003, 22: 597-602.

Chong K.T., Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., AFMP2 Encodes a Novel Immunogenic Protein of the Antigenic Mannoprotein Superfamily in Aspergillus fumigatus. , Journal of Clinical Microbiology . 2004, 42: 2287-91.

Lau S.K.P., Woo P.C.Y., Mok M.Y., Teng L.L., Tam V.K., Chan K.K. and Yuen K.Y., Characterization of Haemophilus segnis, an important cause of bacteremia, by 16S rRNA gene sequencing, Journal of Clinical Microbiology . 2004, 42: 877-80.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Lau S.K.P., Woo P.C.Y., Woo G.K.S., Fung A.M.Y., Wong M.K.M., Chan K.M., Tam D.M.W. and Yuen K.Y., Eggerthella hongkongensis sp. nov. and Eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia, Diagnostic Microbiology and Infectious Disease. Elsevier, 2004, 49: 255-263.

Lau S.K.P., Woo P.C.Y., To A.P.C., Wong T.K. and Yuen K.Y., Lack of Evidence for DDNA in antibiotic preparations as source of antibiotic resistance genes, 14th European Congress of Clinical Microbiology and infectious Diseases. Prague, The Czech Republic.. 2004.

Lau S.K.P., Woo P.C.Y., Yim T.C., To A.P.C. and Yuen K.Y., Molecular characterization of a strain of ngroup a streptococcus isolated from a patient with a psoas abscess, Journal of Clinical Microbiology . 2003, 41: 4888-91.

Lau S.K.P., Woo P.C.Y., Hui W.T., Li M.W., Teng L.L., Que T.L., Luk W.K., Lai R.W., Yung R.W. and Yuen K.Y., Use of cefoperazone MacConkey agar for selective isolation of Laribacter hongkongensis, Journal of Clinical Microbiology . 2003, 41: 4839-41.

Lee R.A., Woo P.C.Y., To A.P.C., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Geographical difference of disease association in Streptococcus bovis bacteraemia, Journal of Medical Microbiology . 2003, 52: 903-8.

Woo P.C.Y., Fung A.S.M., Lau S.K.P., Teng L.L., Wong B.H., Wong M.K., Hon E.H.C., Tang G.W.K. and Yuen K.Y., Actinomyces hongkongensis sp. nov. a novel Actinomyces species isolated from a patient with pelvic actinomycosis, Systematic and Applied Microbiology . 2003, 26(4): 518-22.

Woo P.C.Y., To A.P.C., Tse H., Lau S.K.P. and Yuen K.Y., Clinical and molecular epidemiology of erythromycin-resistant beta-hemolytic lancefield group G streptococci causing bacteremia, Journal of Clinical Microbiology . 2003, 41: 5188-91.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Chan K.H., Tam V.K., Peiris J.S.M. and Yuen K.Y., Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnsosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2004, 42(5): 2306-9.

Woo P.C.Y., Tam D.M., Lau S.K.P., Fung A.M. and Yuen K.Y., Enterococcus cecorum empyema thoracis successfully treated with cefotaxime, Journal of Clinical Microbiology . 2004, 42: 919-22.

Woo P.C.Y., To A.P.C., Lau S.K.P. and Yuen K.Y., Facilitation of horizontal transfer of antimicrobial resistance by transformation of antibiotic-induced cell-wall-deficient bacteria, Medical Hypotheses . 2003, 61: 503-8.

Woo P.C.Y., Lau S.K.P., Fung A.M., Chiu S.K., Yung R.W. and Yuen K.Y., Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology . 2003, 56: 690-3.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Woo P.C.Y., Kuhnert P., Burnens A.P., Teng L.L., Lau S.K.P., Que T.L., Yau H.H. and Yuen K.Y., Laribacter hongkongensis: a potential cause of infectious diarrhea, Diagnostic Microbiology and Infectious Disease . 2003, 47: 551-6.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Woo P.C.Y., Tse H., Chan K.M., Lau S.K.P., Fung A.M., Yip K.T., Tam D.M., Ng K.H., Que T.L. and Yuen K.Y., Streptococcus milleri" endocarditis caused by Streptococcus anginosus, Diagnostic Microbiology and Infectious Disease . 2004, 48: 81-8.

Woo P.C.Y., Zhen H., Cai J.J., Yu J., Lau S.K.P., Wang J., Teng L.L., Wong S.S.Y., Tse R.H., Chen R., Yang H., Liu B. and Yuen K.Y., The mitochondrial genome of the thermal dimorphic fungus Penicillium marneffei is more closely related to those of molds than yeasts, FEBS Letters . 2003, 555: 469-77.

Woo P.C.Y., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Two cases of continuous ambulatory peritoneal dialysis-associated peritonitis due to Plesiomonas shigelloides, Journal of Clinical Microbiology . 2004, 42: 933-5.

Researcher : Lee RA

List of Research Outputs

Lee R.A., Woo P.C.Y., To A.P.C., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Geographical difference of disease association in Streptococcus bovis bacteraemia, Journal of Medical Microbiology . 2003, 52: 903-8.

Researcher : Lee SS

List of Research Outputs

Wong A.T., Tsang O.T., Wong M.Y., Lim W.L., Zheng B., Lee S.S., Lai S.T., Yuen K.Y., Choi K.W., Tso E.Y., Chau T.N., Tong W.L., Chiu M.C. and Yu W.C., PMH SARS Study Group. Coronavirus infection in an AIDS patient, AIDS. 2004, 18: 829-30.

Zheng B., Guan Y., Wong K.H., Zhou J., Wong K.L., Young B.W.Y., Lu L. and Lee S.S., SARS-related virus predating SARS outbreak, Hong Kong, Emerging Infectious Diseases. 2004, 10(2): 176-178.

Zheng B., Zhou J., Qu D., Siu K.L., Lam T.W., Lo H.Y., Lee S.S. and Wen Y.M., Selective Functional Deficit in Dendritic CEll - T Cell interaction Is a Crucial Mechanism in Chronic Hepatitis B Virus Infection, Journal of Viral Hepatitis. 2004, 11: 217-224.

Researcher : Lee TSW

List of Research Outputs

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Researcher : Leung CSW

List of Research Outputs

Guan Y., Peiris J.S.M., Zheng B., Poon L.L.M., Chan K.H., Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Leung C.S.W., Yuen K.Y. and Leung F.C.C., Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome, The Lancet. 2004, 363: 99-104.

Researcher : Leung CYH

List of Research Outputs

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Researcher : Leung ETY

List of Research Outputs

Leung E.T.Y., Kam K.M., Chiu A., Ho P.L., Seto W.H., Yuen K.Y. and Yam W.C., Detection of katG Ser315Thr substitution in respiratory specimens from patients with isoniazid-resistant Mycobacterium tuberculosis using PCR-RFLP, Journal of Medical Microbiology. 2003, 52(Pt 11): 999-1003.

Researcher : Li PH

List of Research Outputs

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Zhong N.S., Zheng B., Li Y.M., Poon L.L.M., Xie Z.H., Chan K.H., Li P.H., Li P.H., Li P.H., Tan S.Y., Chang Q., Xie J.P., Liu X.Q., Xu J., Li D.X., Yuen K.Y., Peiris J.S.M. and Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003, The Lancet. 2003, 362(9393): 1353-8.

Researcher : Lim WWL

List of Research Outputs

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Horinoto T., Fukuda N., Iwatsuki-Horimoto K., Guan Y., Lim W.W.L., Peiris J.S.M., Sugii S., Odagiri T., Tashiro M. and Kawaoka Y., Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003, Journal of Veterinary Medical Science. 2004, 66(3): 303-5.

Kuiken T., Fouchier R.A., Schutten M., Rimmelzwaan G.F., van Amerongen G., van Riel D., Laman J.D., de Jong T., van Doornum G., Lim W.W.L., Ling A.E., Chan P.K., Tam J.S., Zambon M.C., Gopal R., Drosten C., van der Werf S., Escriou N., Manuguerra J.C., Stohr K., Peiris J.S.M. and Osterhaus A.D., Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome., The Lancet. 2003, 362 (9380): 263-70.

Martina B.E., Haagmans B.L., Kuiken T., Fouchier R.A., Rimmelzwaan G.F., Van Amerongen G., Peiris J.S.M., Lim W.W.L. and Osterhaus A.D., Virology: SARS virus infection of cats and ferrets., Nature. 2003, 425(6961): 915.

Peiris J.S.M., Yu W.C., Leung C.W., Cheung C.Y., Ng W.F., Nicholls J.M., Ng T.K., Chan K.H., Lai S.T., Lim W.W.L., Yuen K.Y. and Guan Y., Re-emergence of fatal human influenza A subtype H5N1 disease, Lancet. 2004, 363(9409): 617-619.

Researcher : Luk W

List of Research Outputs

Poon L.L.M., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S.M. and Guan Y., Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). , Clin Chem. 2003, 49: 953-5.

Researcher : Luk WK

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Hui W.T., Li M.W., Teng L.L., Que T.L., Luk W.K., Lai R.W., Yung R.W. and Yuen K.Y., Use of cefoperazone MacConkey agar for selective isolation of Laribacter hongkongensis, Journal of Clinical Microbiology . 2003, 41: 4839-41.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Researcher : Luo S

List of Research Outputs

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Researcher : Ng IHY

List of Research Outputs

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Researcher : Ng LWY

List of Research Outputs

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Researcher : Ng SP

Project Title:	Monoclonal antibodies against lipopolysaccharide antigens of Salmonella and Shigella spp.
Investigator(s):	Dr. Ng S.P.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	02/2001

Abstract:

To characteerise a panel of monoclonal antibodies raised against the outer core region of Salamonella lipopolysaccharides (LPS).

Project Title:	Collection of background information on the alternative faecal indicators in the environmental waters of Hong Kong and their correlation with Escherichia coli
Investigator(s):	Dr. Ng S.P., Dr. Yam W.C.
Department:	Microbiology
Source(s) of Funding:	Environment Protection Department, Hong Kong Government
Start Date:	06/2001

Abstract:

To detect and enumerate alternative faecal microbial indicator organisms in the environmental waters of Hong Kong and to correlate the results with Escherichia coli in order to assess their value in water quality monitoring.

Project Title:	Sero-prevalence of viral hepatitis A in a sample Hong Kong population
Investigator(s):	Dr. Ng S.P., Dr. Wong K.H.
Department:	Microbiology
Source(s) of Funding:	Health Department of Hong Kong Government
Start Date:	06/2001

Abstract:

To assess the exposure of Hong Kong population to viral hepatitis A by surveying serum antibody against HAV.

List of Research Outputs

Chan K.L., Ng S.P., Chan K.W., Wo Y.H. and Tam P.K.H., Pathogenesis of neonatal necrotizing enterocolitis: a study of the role of intraluminal pressure, age and bacterial concentration, Pediatric Surgery International. 2003, 19(8): 573-577.

Researcher : Ng WWS

List of Research Outputs

Leung P.H., Peiris J.S.M., Ng W.W.S., Robins-Browne R.M., Bettelheim K.A. and Yam W.C., A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli., Applied and Environmental Microbiology. 2003, 69(12): 7549-53.

Researcher : Peiris JSM

Project Title:	Influenza pandemic preparedness in Asia (Research collaboration with national Institute of Allergy and Infectious Diseases (NIAID), St. Jude Children's Research Hospital, Inc. (SJCRH) and The University of Hong Kong)
Investigator(s):	Prof. Peiris J.S.M., Dr. Guan Y.
Department:	Microbiology
Source(s) of Funding:	National Institutes of Health through World Health Organization
Start Date:	04/1999

Abstract:

To study influenza pandemic preparedness in Asia.

Project Title:	Human herpesvirus 6 (HHV-6) and HHV-7 infections: reactivation, latency and viral interactions
Investigator(s):	Prof. Peiris J.S.M., Dr. Chiu S.S.S.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	11/2001

Abstract:

To investigate patients with primary HHV-6 or HHV-7 inection, lateny and reactivation to: a) define the transcription patterns of viral genes in these different states, b) study the effect of related herpesviral infections on the reactivation of HHV-6, HHV-7 and CMV from latency in-vivo. To establish in-vitro models of HHV-6 and HHV-7 latency and reactivation to a) define the transcription patterns of viral genes in productive replication, latency and reactivation, b) study the effect of viral interactions on reactivation from latency, c) study the effect of cross-reacting antibody on the establishment of infection and latency in monocytes and peripheral blood leukocytes.

Project Title:	The Zoonotic potential of influenze viruses in Southeastern China
Investigator(s):	Prof. Peiris J.S.M., Dr. Guan Y., Prof. Shortridge K.F., Dr. Webster R.G.
Department:	Microbiology
Source(s) of Funding:	Wellcome Trust / Australian / New Zealand International Collaborative Research Grant
Start Date:	05/2002

Abstract:

To establish the epidemiological dynamics and a spatially structured ecological / epidemiological model of influenza in poultry and pigs in Hong Kong and mainland China that provide conditions for interspecies transmission of influenza viruses; to determine if certain genotypes of influenza viruses have pandemic potential.

Project Title:	The application of DNA arrays to characterise the avian influenza gene pool in southern China
Investigator(s):	Prof. Peiris J.S.M., Dr. Guan Y., Prof. Li K.S., Prof. Shortridge K.F., Dr. Yang M.M.S.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2002

Abstract:

This project is a multi-institutional collaborative study for developing a high throughput DNA array to allow genotyping of influenza virus isolates of avian origin from Hong Kong and applying it to characterize the influenza virus gene pool in sourthern China. Through its application, it is expected to:- 1) identify the precursor virus that donated its internal genes to generate the mutiple reassortment of H5N1 isolated in Hong Kong markets in 2001; 2) define the frequency of reassortment between viruses of different virus lineages that co-circulate in poultry in Hong Kong and correlate this to host-specificity; and 3) investigate whether avian internal gene segments are transiently introduced into the human influenza gene pool.

Project Title:	Virus-cell interaction and the pathogenesis of SARS
Investigator(s):	Prof. Peiris J.S.M.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To explore what is the biological basis for the selective attraction of macrophages to the lung; to study what immunopathological consequences follow.

Project Title:	Genetic variation of the SARS coronavirus: molecular epidemiology and antigenic variation
Investigator(s):	Prof. Peiris J.S.M., Prof. Anderson R.M., Dr. Chan P.K.S., Dr. Guan Y., Prof. Hedley A.J., Prof. Lam T.H., Dr. Leung G.M., Dr. Leung F.C.C., Dr. Lim W.W.L., Dr. Poon L.L.M., Prof. Sung J.J.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	08/2003

Abstract:

To establish a comprehensive integrated clinical specimen and viral genetic sequence database on SARS-CoV in Hong Kong; to define the molecular epidemiology of the virus and transmission patterns within Hong Kong and the wider region; to define the genetic diversity in the S-gene region associated with antigenic change.

Project Title:	Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?
Investigator(s):	Prof. Peiris J.S.M., Dr. Guan Y., Dr. Lau A.S.Y., Dr. Poon L.L.M.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003

Abstract:

To identify the viral genetic determinants responsible for the differential cytokine hyper-induction associated with H5N1/97 viruses. We have already established the role of the viral NS gene, however, a role for other viral genes has not been excluded. we hypothesize (and have preliminary evidence) that one or more other viral genes are also involved in the hyper-induction of cytokines; to determine whether the avian viral genes/gene products of H5N1/97 directly up-regulate cytokine induction or lack "immune evasion" mechanisms evolved by human influenza viruses? to determine whether the differential up-regulation of cytokines associated with H5N1/97 is also observed in human lung epithelial cells (primary bronchial epithelial cells and human lung epithelial cell lines e.g. A549 cells.

Project Title:	Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?
Investigator(s):	Prof. Peiris J.S.M., Dr. Guan Y., Dr. Lau A.S.Y., Dr. Poon L.L.M.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	09/2003

Abstract:

List of Research Outputs

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Chan K.S., Lai S.T., Chu C.M., Tsui E., Tam C.Y., Wong M.M., Tse M.W., Que T.L., Peiris J.S.M., Sung J., Wong V.C. and Yuen K.Y., Treatment of severe acute respiratory syndrome with lopinavir/ritonavir: a multicentre retrospective matched cohort study., Hong Kong Medical Journal. 2003, 9(6): 399-406.

Chan K.Y.K., Song Y., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W.C., Chan L.C., Tam P.K.H., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 (ANPEP) genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong., The Human Genome organisation Meeting, April 2004, Berlin, Germany. 2004, Abstract No. 159.

Chan Y.K., Song Y.Q., Mak W., Cheung A.N.Y., Peiris J.S.M., Yip S.P., Tin V., Liu W., Xue W., Chan L.C., Tam P., Ngan H.Y.S. and Khoo U.S., Polymorphisms of the ACE2 and CD13 genes, and susceptibility to SARS coronavirus infection in Chinese from Hong Kong, Abstract for Human Genome Organization (HUGO) Meeting, April 4-7, 2004, Berlin, Germany. 2004.

Cheng V.C.C., Hung I.F.N., Tang B.S., Chu C.M., Wong M.M., Chan K.H., Wu A.K., Wu A.K., Tse D.M., Chan K.S., Zheng B., Peiris J.S.M., Sung J.J. and Yuen K.Y., Viral replication in the nasopharynx is associated with diarrhea in patients with severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(4): 467-75.

Chiu S.S.S., Lau Y.L., Chan K.H., Wong W.H.S. and Peiris J.S.M., The global implications of influenza in Hong Kong, The 11th Asian Congress of Pediatrics & The 1st Asian Congress on Pediatric Nursing, Bangkok, Thailand, 2-7 November 2003.

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Chung B.H.Y., Chan G.C.F., Peiris J.S.M., Ha S.Y., Lee T.L. and Lau Y.L., Recurrent disseminated varicella infectionin paediatric oncology patients, Joint Annual Scientific Meeting 2003 of the Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association Limited, QEH, Hong Kong, 13 December 2003. 88.

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Guan Y., Peiris J.S.M., Zheng B., Poon L.L.M., Chan K.H., Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Leung C.S.W., Yuen K.Y. and Leung F.C.C., Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome, The Lancet. 2004, 363: 99-104.

Guan Y., Peiris J.S.M., Poon L.L.M., Dyrting K.C., Ellis T.M., Sims L., Webster R.G. and Shortridge K.F., Reassortants of H5N1 influenza viruses recently isolated from aquatic poultry in Hong Kong SAR., Avian Diseases. 911-3, 2003, 47(3 Suppl).

Horinoto T., Fukuda N., Iwatsuki-Horimoto K., Guan Y., Lim W.W.L., Peiris J.S.M., Sugii S., Odagiri T., Tashiro M. and Kawaoka Y., Antigenic differences between H5N1 human influenza viruses isolated in 1997 and 2003, Journal of Veterinary Medical Science. 2004, 66(3): 303-5.

Hui K.H., Zeng F., Chan M.N., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., RT-PCR Diagnosis of Coronavirus Associated with Severe Acute Respiratory Syndrome (SARS), Journal Clinical Microbiology. 2004, 42: 1994-1999.

Hui R.K., Zeng F., Chan C.M., Yuen K.Y., Peiris J.S.M. and Leung F.C., Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome, Journal of Clinical Microbiology . 2004, 42: 1994-9.

Jones B.M., Ma E.S.K., Peiris J.S.M., Wong P.C., Ho J.C.M., Lam B., Lai K.N. and Tsang K.W.T., Prolonged disturbances of in vitro cytokine production in patients with severe acute respiratory syndrome (SARS) treated with ribavirin and steroids, Clinical & Experimental Immunology. 2004, 135: 467-473.

Kuiken T., Fouchier R.A., Schutten M., Rimmelzwaan G.F., van Amerongen G., van Riel D., Laman J.D., de Jong T., van Doornum G., Lim W.W.L., Ling A.E., Chan P.K., Tam J.S., Zambon M.C., Gopal R., Drosten C., van der Werf S., Escriou N., Manuguerra J.C., Stohr K., Peiris J.S.M. and Osterhaus A.D., Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome., The Lancet. 2003, 362 (9380): 263-70.

Kung N.Y., Guan Y., Perkins N.R., Bissett L., Ellis T., Sims L., Morris R.S., Shortridge K.F. and Peiris J.S.M., The impact of a monthly rest day on avian influenza virus isolation rates in retail live poultry markets in Hong Kong., Avian Diseases. 2003, 47(3 Suppl): 1037-41.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Lee D.C.W., Li C.B., Law H.Y., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., A Tale of Two Viruses: Cytokine Dysregulation in HIV and H5N1 Infections, 3^rd International Proteomics Conference (IPC'03), 1^st Taiwan Proteomics Conference, Joint Meeting of AOHUPO 2004, Taipei, Taiwan, 14-17 May 2004.

Leung C.W., Kwan Y.W., Ko P.W., Chiu S.S.S., Loung P.Y., Fong N.C., Lee L.P., Hui Y.W., Law H.K.W., Wong W.H.S., Chan K.H., Peiris J.S.M., Lim W.W.L., Lau Y.L. and Chiu M.C., Severe acute respiratory syndrome among children, Pediatrics. 2004, 113(6): e535-e543.

Leung G.M., Chung P.H., Tsang T., Lim W., Chan K.K., Chau Y.K., Donnelly C.A., Ghani A., Fraser C., Riley S., Ferguson N.M., Anderson R.M., Law Y.L., Mok T., Ng T., Fu A., Leung P.Y., Peiris J.S.M., Lam T.H. and Hedley A.J., Seroprevalence of IgG antibody to SARS coronavirus (SARS-CoV) in a population-based sample of close contacts of all 1755 cases in Hong Kong (Abstract), Hong Kong SARS Forum and Hospital Authority Convention 2004, 8-11 May 2004, Hong Kong. Hong Kong, Hospital Authority, 2004, 84.

Leung P.H., Peiris J.S.M., Ng W.W.S., Robins-Browne R.M., Bettelheim K.A. and Yam W.C., A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli., Applied and Environmental Microbiology. 2003, 69(12): 7549-53.

Li C.B., Lee D.C.W., Law H.Y., Peiris J.S.M. and Lau A.S.Y., Kinase Signaling Pathways in HIV Infections, Frontiers in Biomedical Research 2003, HKU, 12 December 2003. 60.

Liu M., Guan Y., Peiris J.S.M., He S., Webby R.J., Perez D. and Webster R.G., The quest of influenza A viruses for new hosts, Avian Diseases. 2003, 47(3 Suppl): 849-56.

Martina B.E., Haagmans B.L., Kuiken T., Fouchier R.A., Rimmelzwaan G.F., Van Amerongen G., Peiris J.S.M., Lim W.W.L. and Osterhaus A.D., Virology: SARS virus infection of cats and ferrets., Nature. 2003, 425(6961): 915.

Peiris J.S.M., 12th T.B. Teoh Foundation Lecture: Bird Flu-SARS, What's next?, Scientific Seminar, Hong Kong College of Pathologists. 2003.

Peiris J.S.M., Avian Influenza A (H5N1) virus: The Hong Kong experience, The Malaysian Society of Infectious Diseases and Chemotherarpy with Asia Pacific Society for Medical Virology and Institute for Medical Research Seminar on Avian Influenza. 2004.

Peiris J.S.M., Avian Influenza viruses and human disease, 6th Asia Pacific Congress of Medical Virology, Kuala Lumpur, Malaysia. 2003.

Peiris J.S.M., Avian influenza A (H5N1) virus: The Hong Kong experience, Singapore ID Society Meeting on Infectious Disease (Practice Update 2004). 2004.

Peiris J.S.M., Tang W.H., Chan K.H., Khong P.L., Guan Y., Lau Y.L. and Chiu S.S.S., Children with Respiratory Disease associated with Human Metapneumovirus in Hong Kong, Symposium on Legionnaires' Disease and other Respiratory Infections and their Prevention, HK, 12 December 2003.

Peiris J.S.M., Emerging Viral Infections in the Asian Region, The Hong Kong Society for Infectious Diseases Eight Annual Scientific Meeting, Hong Kong. 2004.

Peiris J.S.M., H5 Animal to Human Interface, Regional Workshop on Human Influenza A (H5N1) Detection and Control, Thailand. 2004.

Peiris J.S.M., Infectious disease: Emerging Viral Infections, "Advances in Medicine 2003" by Faculty of Medicne, The Chinese University of Hong Kong. 2003.

Peiris J.S.M., Observations from the Epicenter, VIth International Symposium on Respiratory Vial Infections, Florida, USA. 2004.

Peiris J.S.M., Plenary Session: SARS Epidemic Topic: SARS: A new emerging disease, 2nd Asian American International Medical Society's Annual Meeting. 2004.

Peiris J.S.M., Yu W.C., Leung C.W., Cheung C.Y., Ng W.F., Nicholls J.M., Ng T.K., Chan K.H., Lai S.T., Lim W.W.L., Yuen K.Y. and Guan Y., Re-emergence of fatal human influenza A subtype H5N1 disease, Lancet. 2004, 363(9409): 617-619.

Peiris J.S.M., Role of laboratory services in outbreak control, HKU School of Public Health: Infectious Disease Epidemiology and Control. 2003.

Peiris J.S.M., SARS and other emerging infections, Ceylon College of Physicians - Annual Academic Sessions 2003.

Peiris J.S.M., SARS: A New Emerging Disease, Viral Vaccine Meeting, Barcelona, Spain. 2003.

Peiris J.S.M., SARS: A new emerging disease, 6th Asia Pacific Congress of Medical Virology, Kuala Lumpur, Malaysia. 2003.

Peiris J.S.M., SARS: Aetiology, pathogenesis and transmission, SARS and viral respiratory infections: Looking ahead. 2003.

Peiris J.S.M., SARS: An emerging disease, IX International Antiviral Symposium and Workshop, Antalya, Turkey. 2003.

Peiris J.S.M., SARS: Emerging Infections, 2nd International Congress of APSIC, Singapore. 2004.

Peiris J.S.M., SARS: The hunt for the aetiology, Symposium on SARS Research and Diagnostics Advances. 2003.

Peiris J.S.M., SARS: confronting a new disease, The Royal Society of London Scientific Discussion Meeting Emerging infections: what have we learnt from SARS. 2004.

Peiris J.S.M., Session: Emerging Infectious Diseases: Topic: Severe acute respiratory syndrome, Hong Kong Academy of Medicine, Hong Kong. 2003.

Peiris J.S.M., Severe Acute Respiratory Syndrome (SARS), Journal of Clinical Virology. 2003, 28(3): 245-7.

Peiris J.S.M., Special Opening Lecture on SARS, 43rd ICAAC Meeting, Chicago, USA. 2003.

Peiris J.S.M., The Gardner Lecture: Emerging infections from the Asian Region, ESCV Winter Meeting Copenhagen 2004.

Peiris J.S.M., The pathogenesis of human influenza A (H5N1), Options for the Control of Influenza V, Japan. 2003.

Peiris J.S.M., Yuen K.Y., Osterhaus A.D. and Stohr K., The severe acute respiratory syndrome., The New England Journal of Medicine. 2003, 349(25): 2431-41.

Peiris J.S.M., The surveillance and control of avian flu in Asia, 2004 Conference on Pandemic Influenza Preparedness, Taiwan. 2004.

Peiris J.S.M., The virus - diagnosis and survival properties, 2nd International Congress of APSIC, Singapore. 2004.

Peiris J.S.M., Viral Infection, Asia Pacific Society of Infection Control, Hong Kong. 2003.

Poon L.L.M., Chan K.H., Nicholls J.M., Zheng B., Yuen K.Y., Guan Y. and Peiris J.S.M., Characterization of a novel coronavirus responsible for severe acute respiratory syndrome, Options for the Control of Influenza V. 2004, International Congress Series 1263: 805-808.

Poon L.L.M., Chan K.H. and Peiris J.S.M., Crouching tiger, hidden gragon: the laboratory diagnosis of severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(2): 297-9.

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Poon L.L.M., Chan K.H., Wong O.K., Yam W.C., Yuen K.Y., Guan Y., Lo Y.M. .D.E.N.N.I.S. and Peiris J.S.M., Early diagnosis of SARS Coronavirus infection by real time RT-PCR, Journal of Clinical Virology. 2003, 28(3): 233-8.

Poon L.L.M., Leung C.S., Tashiro M., Chan K.H., Wong B.W., Yuen K.Y., Guan Y. and Peiris J.S.M., Rapid Detection of the Severe Acute Respiratory Syndrome (SARS) Coronavirus by a Loop-Mediated Isothermal Amplification Assay, Clinical Chemistry . 2004, 50: 1050-52.

Poon L.L.M., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S.M. and Guan Y., Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). , Clin Chem. 2003, 49: 953-5.

Shek C.C., Ng P.C., Fung G.P., Cheng F.W., Chan P.K., Peiris J.S.M., Lee K.H., Wong S.F., Cheung H.M., Li A.M., Hon E.K., Yeung C.K., Chow C.B., Tam J.S., Chiu M.C. and Fok T.F., Infants born to mothers with severe acute respiratory syndrome., Pediatrics. 2003, 112(4): e254.

Sims L.D., Guan Y., Ellis T.M., Liu K.K., Dyrting K., Wong H., Kung N.Y., Shortridge K.F. and Peiris J.S.M., An update on avian influenza in Hong Kong 2002., Avian Diseases. 2003, 47(3 Suppl): 1083-6.

Sims L.D., Ellis T.M., Liu K.K., Dyrting K., Wong H., Peiris J.S.M., Guan Y. and Shortridge K.F., Avian Influenza in Hong Kong 1997-2002., Avian Diseases. 2003, 47(3 Suppl): 832-838.

Sturm-Ramirez K.M., Ellis T., Bousfield B., Guan Y., Peiris J.S.M. and Webster R.G., H5N1 influenza A viruses from 2002 are highly pathogenic in waterfowl, Options for the Control of Influenza V. 2004, International Congress Series 1263: 200-204.

Sturm-Ramirez K.M., Ellis T., Bousfield B., Bissett L., Dyrting K., Rehg J.E., Poon L.L.M., Guan Y., Peiris J.S.M. and Webster R.G., Reemerging H5N1 influenza viruses in Hong Kong in 2002 are highly pathogenic to ducks., Journal of Virology. 2004, 78(9): 4892-901.

Tong C.Y., Muir P., Bakran A., Peiris J.S.M. and Herrington C.S., The association of viral infection and chronic allograft nephropathy with graft dysfunction after renal transplantation. , Transplantation. 2003, 76(3): 621-2.

Webby R.J., Perez D.R., Coleman J.S., Guan Y., Knight J.H., Govorkova E.A., McClain-Moss L.R., Peiris J.S.M., Rehg J.E., Tuomanen E.I. and Webster R.G., Responsiveness to a pandemic alert: use of reverse genetics for rapid development of influenza vaccines., The Lancet. 2004, 363(9415): 1099-103.

Wong C.M., Chan K.P., Hedley A.J. and Peiris J.S.M., Disease burden of influenza on hospitalization and mortality, Frontiers in Biomedical Research HKU 2003, 12 December 2003, Hong Kong. 2003, 61.

Wong C.M., Peiris J.S.M., Hedley A.J., Thomas G.N., Thach T.Q., Lam T.H. and Wong T.W., Interaction between air pollution and respiratory viruses: Time-series studies for daily mortality and hospital admissions (Abstract), Health Effects Institute Annual Conference 2004, 2-4 May 2004, Boston, USA. Boston, Health Effects Institute, 2004, 38.

Wong C.M., Peiris J.S.M., Hedley A.J., Thomas G.N., Thach T.Q., Lam T.H. and Wong T.W., Interaction between air pollution and respiratory viruses: Time-series studies for daily mortality and hospittal admissions (presented in conference), Pearl River Delta Regional Workshop: Current air quality, meteorological & health issues, 1-2 June 2004, Guangzhou. 2004.

Wong C.M., Chan K.P. and Peiris J.S.M., The impact of influenza predominance epidemics on mortality in a sub-tropical region, 15th Conference of the International Society for Environmental Epidemiology, 23-26 September 2003, Perth. Perth, Western Australia, 2003, S53.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Chan K.H., Tam V.K., Peiris J.S.M. and Yuen K.Y., Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnsosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2004, 42(5): 2306-9.

Yam W.C., Chan K.H., Poon L.L.M., Guan Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus, Journal of Clinical Microbiology. 2003, 41(10): 4521-4524.

Zeng F., Chow Y.C.K., Hon C.C., Law K.M., Yip C.W., Chan K.H., Peiris J.S.M. and Leung F.C.C., Characterization of humoral responses in mice immunized with plasmid DNAs encoding SARS-CoV spike gene fragments, Biochemical and Biophysical Research Communications. 2004, 315: 1134-1139.

Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Wang Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., The Complete Genome Sequence of Severe Acute Respiratory Syndrome Coronavirus Strain HKU-39849 (HK-39), Experimental Biology Medicine. 2003, 228: 866-873.

Zhong N.S., Zheng B., Li Y.M., Poon L.L.M., Xie Z.H., Chan K.H., Li P.H., Li P.H., Li P.H., Tan S.Y., Chang Q., Xie J.P., Liu X.Q., Xu J., Li D.X., Yuen K.Y., Peiris J.S.M. and Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003, The Lancet. 2003, 362(9393): 1353-8.

Zhou J., Law H.K.W., Yeung C.Y., Peiris J.S.M. and Lau Y.L., Infection of neonatal and adult macrophages by influenza A viruses 54/98 (H1N1) and G1/97 (H9N2), 8^th Research Postgraduate Symposium, Hong Kong, 13 December 2003. 76.

Researcher : Poon LLM

Project Title:	Reverse genetic for a coronavirus responsible for severe acute respiratory syndrome
Investigator(s):	Dr. Poon L.L.M., Dr. Guan Y.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To generate a coronavirus-like mini vRNA GFP (green fluorescent protein) reporter system to study the transcription and replication of coronavirus; to establish a reverse genetic system of coronavirus.

Project Title:	Rapid molecular diagnosis of SARS coronavirus
Investigator(s):	Dr. Poon L.L.M., Prof. Lo D.Y.M., Prof. Peiris J.S.M., Prof. Tam J.S.L.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	08/2003
Completion Date:	07/2004

Abstract:

To refine existing PCR-based assays for SARS-CoV detection; to develop novel quantitative PCR-based assays which target at other regions in the SARS-CoV genome; to identify the optimal clinical samples for SARS-CoV diagnosis; to determine the quantity of various viral RNA species (i.e. genomic, subgenomic RNA and defective interference viral particle) in clinical samples; to apply the quantitative PCR assays to determine the viral dynamic in SARS patients at different time points; to quantitative the amount of SARS-CoV in atypical SARS patients, such as asymptomatic patients and 'super-spreaders'.

Project Title:	Roles of segment-specific vRNA sequences of influenza A virus in viral replication, transcription and gene expression
Investigator(s):	Dr. Poon L.L.M., Dr. Guan Y., Prof. Peiris J.S.M.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	11/2003

Abstract:

To establish a GFP reporter assay for influenza replication studies; to determine the potential function of segment-specific viral RNA sequences in viral replication, tansporation and gene expression; to study the 5' and 3' segment-specific regions of avian, human and swine influenza.

Project Title:	Roles of segment-specific vRNA sequences of influenza A virus in viral replication, transcription and gene expression
Investigator(s):	Dr. Poon L.L.M., Dr. Guan Y., Prof. Peiris J.S.M.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	11/2003

Abstract:

Project Title:	Use of small molecules as antiviral drugs for influenza A viruses
Investigator(s):	Dr. Poon L.L.M., Dr. Kao R.Y.T.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	07/2004

Abstract:

The recent use of high-throughput screening (HTS) to search for small molecule inhibitors provides a new possibility of identifying small molecules with anti-influenza effects. This approach has been proved to be useful in finding small molecule inhibitors of angiogenin. We intend to apply HTS technology to identify small molecules to inhibit influenza virus replication. In particular, we intend to identify inhibitors that are specific for the viral polymerase subunits.

List of Research Outputs

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Guan Y., Peiris J.S.M., Zheng B., Poon L.L.M., Chan K.H., Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Leung C.S.W., Yuen K.Y. and Leung F.C.C., Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome, The Lancet. 2004, 363: 99-104.

Guan Y., Peiris J.S.M., Poon L.L.M., Dyrting K.C., Ellis T.M., Sims L., Webster R.G. and Shortridge K.F., Reassortants of H5N1 influenza viruses recently isolated from aquatic poultry in Hong Kong SAR., Avian Diseases. 911-3, 2003, 47(3 Suppl).

Ng E.K., Ng P.C., Hon K.L., Cheng W.T., Hung E.C., Chan K.C., Chiu R.W., Li A.M., Poon L.L.M., Hui D.S., Tam J.S., Fok T.F. and Lo Y.M., Serial Analysis of the Plasma Concentration of SARS Coronavirus RNA in Pediatric Patients with Severe Acute Respiratory Syndrome, Clinical Chemistry . 2003, 49: 2085-2088.

Poon L.L.M., Animal/Avian influenza and pandemic disease., in APEC pandemic influenza preparedness meeting, Kuala Lumpur, Malaysia.. 2003.

Poon L.L.M., Chan K.H., Nicholls J.M., Zheng B., Yuen K.Y., Guan Y. and Peiris J.S.M., Characterization of a novel coronavirus responsible for severe acute respiratory syndrome, Options for the Control of Influenza V. 2004, International Congress Series 1263: 805-808.

Poon L.L.M., Chan K.H. and Peiris J.S.M., Crouching tiger, hidden gragon: the laboratory diagnosis of severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(2): 297-9.

Poon L.L.M., Detection of SARS coronavirus in animals and in humans., in International Conference on SARS - one year after the (first) outbreak, Lueberk, Germany.. 2004.

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Poon L.L.M., Chan K.H., Wong O.K., Yam W.C., Yuen K.Y., Guan Y., Lo Y.M. .D.E.N.N.I.S. and Peiris J.S.M., Early diagnosis of SARS Coronavirus infection by real time RT-PCR, Journal of Clinical Virology. 2003, 28(3): 233-8.

Poon L.L.M., Identification and characterization of a coronavirus causing severe acute respiratory syndrome., in the International Meeting of the Federation of Korean Microbiological Societies, Seoul, Korea.. 2003.

Poon L.L.M., In the WHO Animal Influenza Training Course, HKU, Hong Kong., 2004.

Poon L.L.M., In the WHO Regional H5N1 Laboratory and Epidemiology Training Course, Bangkok, Thailand., 2004.

Poon L.L.M., Leung C.S., Tashiro M., Chan K.H., Wong B.W., Yuen K.Y., Guan Y. and Peiris J.S.M., Rapid Detection of the Severe Acute Respiratory Syndrome (SARS) Coronavirus by a Loop-Mediated Isothermal Amplification Assay, Clinical Chemistry . 2004, 50: 1050-52.

Poon L.L.M., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S.M. and Guan Y., Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). , Clin Chem. 2003, 49: 953-5.

Poon L.L.M., SARS: Etiology, diagnostics, transmission and clinical aspects., in the International Workshop on SARS, Tokyo, Japan. . 2004.

Poon L.L.M., SARS diagnosis., in the SARS and viral respiratory infections: Looking ahead, HKU-Pasteur Research Centre, Hong Kong.. 2003.

Snijder E.J., Bredenbeek P.J., Dobbe J.C., Thiel V., Ziebuhr J., Poon L.L.M., Guan Y., Rozanov M., Spaan W.J. and Gorbalenya A.E., Unique and conserved features of genome and proteome of SARS-coronavirus, an early split-off from the coronavirus group 2 lineage , Journal of Molecular Biology. 2003, 331(5): 991-1004.

Sturm-Ramirez K.M., Ellis T., Bousfield B., Bissett L., Dyrting K., Rehg J.E., Poon L.L.M., Guan Y., Peiris J.S.M. and Webster R.G., Reemerging H5N1 influenza viruses in Hong Kong in 2002 are highly pathogenic to ducks., Journal of Virology. 2004, 78(9): 4892-901.

Sutton G., Fry E., Carter L., Sainsbury S., Walter T., Nettleship J., Berrow N., Owens R., Gilbert R., Davidson A., Siddell S., Poon L.L.M., Diprose J., Alderton D., Walsh M., Grimes J.M. and Stuart D.I., The nsp9 replicase protein of SARS-coronavirus, structure and functional insights, Structure . 2004, 12: 341-353.

Yam W.C., Chan K.H., Poon L.L.M., Guan Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus, Journal of Clinical Microbiology. 2003, 41(10): 4521-4524.

Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Wang Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., The Complete Genome Sequence of Severe Acute Respiratory Syndrome Coronavirus Strain HKU-39849 (HK-39), Experimental Biology Medicine. 2003, 228: 866-873.

Zhong N.S., Zheng B., Li Y.M., Poon L.L.M., Xie Z.H., Chan K.H., Li P.H., Li P.H., Li P.H., Tan S.Y., Chang Q., Xie J.P., Liu X.Q., Xu J., Li D.X., Yuen K.Y., Peiris J.S.M. and Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003, The Lancet. 2003, 362(9393): 1353-8.

Researcher : Poon RWS

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Researcher : Que TL

List of Research Outputs

Chan K.S., Lai S.T., Chu C.M., Tsui E., Tam C.Y., Wong M.M., Tse M.W., Que T.L., Peiris J.S.M., Sung J., Wong V.C. and Yuen K.Y., Treatment of severe acute respiratory syndrome with lopinavir/ritonavir: a multicentre retrospective matched cohort study., Hong Kong Medical Journal. 2003, 9(6): 399-406.

Ho P.L., Wong R.C., Chow K.H., Cheung M.Y., Wong S.S.Y., Yam W.C. and Que T.L., Application of a multiplex pbp2b and pbp2x PCR for prediction of penicillin resistance in Streptococcus pneumoniae., Journal of Antimicrobial Chemotherapy. 2004, 53(5): 890-1.

Lau S.K.P., Woo P.C.Y., Hui W.T., Li M.W., Teng L.L., Que T.L., Luk W.K., Lai R.W., Yung R.W. and Yuen K.Y., Use of cefoperazone MacConkey agar for selective isolation of Laribacter hongkongensis, Journal of Clinical Microbiology . 2003, 41: 4839-41.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Woo P.C.Y., Kuhnert P., Burnens A.P., Teng L.L., Lau S.K.P., Que T.L., Yau H.H. and Yuen K.Y., Laribacter hongkongensis: a potential cause of infectious diarrhea, Diagnostic Microbiology and Infectious Disease . 2003, 47: 551-6.

Woo P.C.Y., Tse H., Chan K.M., Lau S.K.P., Fung A.M., Yip K.T., Tam D.M., Ng K.H., Que T.L. and Yuen K.Y., Streptococcus milleri" endocarditis caused by Streptococcus anginosus, Diagnostic Microbiology and Infectious Disease . 2004, 48: 81-8.

Researcher : Seto WH

List of Research Outputs

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Chan M.M.W., Seto W.H., Sung J.J., Lai T.S.T., Yee W., Yam L.Y.C., Tsang K.W.T., Ip M.S.M., Lam W.K. and Lai K.N., Severe Acute Respiratory Syndrome: Patients Were Epidemiologically Linked, British Medical Journal. 2003, 326: 1393.

Ho P.L., Tang X.P. and Seto W.H., SARS: hospital infection control and admission strategies., Respirology. 2003, Suppl: S41-5.

Lai K.N., Tsang K.W.T., Seto W.H. and Ooi C.G.C., Clinical, Laboratory, and Radiologic Manifestation of SARS, Current Infectious Disease Reports. 2004, 6: 213-219.

Lam M.F., Ooi C.G.C., Lam B., Ho J.C.M., Seto W.H., Ho P.L., Wong P.C., Liang R.H.S., Lam W.K. and Tsang K.W.T., An indolent case of severe acute respiratory syndrome, American Journal of Respiratory and Critical Care Medicine. 2004, 169(1): 125-128.

Leung E.T.Y., Kam K.M., Chiu A., Ho P.L., Seto W.H., Yuen K.Y. and Yam W.C., Detection of katG Ser315Thr substitution in respiratory specimens from patients with isoniazid-resistant Mycobacterium tuberculosis using PCR-RFLP, Journal of Medical Microbiology. 2003, 52(Pt 11): 999-1003.

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Tsang K.W.T. and Seto W.H., Severe acute respiratory syndrome - scientific and anecdotal evidence for drug treatment, Current Opinion in Investigational Drugs. 2004, 5(2): 179-185.

Yam W.C., Cheng V.C.C., Hui W.T., Wang L.N., Seto W.H. and Yuen K.Y., Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA), Diagnostic Microbiology and Infectious Disease . 2004, 48: 271-5.

Yam W.C., Chan K.H., Poon L.L.M., Guan Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus, Journal of Clinical Microbiology. 2003, 41(10): 4521-4524.

Researcher : Shortridge KF

List of Research Outputs

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Guan Y., Peiris J.S.M., Poon L.L.M., Dyrting K.C., Ellis T.M., Sims L., Webster R.G. and Shortridge K.F., Reassortants of H5N1 influenza viruses recently isolated from aquatic poultry in Hong Kong SAR., Avian Diseases. 911-3, 2003, 47(3 Suppl).

Kung N.Y., Guan Y., Perkins N.R., Bissett L., Ellis T., Sims L., Morris R.S., Shortridge K.F. and Peiris J.S.M., The impact of a monthly rest day on avian influenza virus isolation rates in retail live poultry markets in Hong Kong., Avian Diseases. 2003, 47(3 Suppl): 1037-41.

Sims L.D., Guan Y., Ellis T.M., Liu K.K., Dyrting K., Wong H., Kung N.Y., Shortridge K.F. and Peiris J.S.M., An update on avian influenza in Hong Kong 2002., Avian Diseases. 2003, 47(3 Suppl): 1083-6.

Sims L.D., Ellis T.M., Liu K.K., Dyrting K., Wong H., Peiris J.S.M., Guan Y. and Shortridge K.F., Avian Influenza in Hong Kong 1997-2002., Avian Diseases. 2003, 47(3 Suppl): 832-838.

Researcher : Siu KL

List of Research Outputs

Zheng B., Zhou J., Qu D., Siu K.L., Lam T.W., Lo H.Y., Lee S.S. and Wen Y.M., Selective Functional Deficit in Dendritic CEll - T Cell interaction Is a Crucial Mechanism in Chronic Hepatitis B Virus Infection, Journal of Viral Hepatitis. 2004, 11: 217-224.

Researcher : Sturm-Ramirez KM

List of Research Outputs

Sturm-Ramirez K.M., Ellis T., Bousfield B., Guan Y., Peiris J.S.M. and Webster R.G., H5N1 influenza A viruses from 2002 are highly pathogenic in waterfowl, Options for the Control of Influenza V. 2004, International Congress Series 1263: 200-204.

Researcher : Tang SF

List of Research Outputs

Cheng V.C.C., Yam W.C., Hung I.F., Woo P.C.Y., Lau S.K.P., Tang S.F. and Yuen K.Y., Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis, Journal of Clinical Pathology . 2004, 57: 281-5.

Cheng V.C.C., Hung I.F.N., Wu A.K., Tang S.F., Chu C.M. and Yuen K.Y., Lymphocyte surge as a marker for immunorestitution disease due to Pneumocystis jiroveci pneumonia in HIV-negative immunosuppressed hosts., European journal of clinical microbiology & infectious diseases. 2004, 23(6): 512-4.

Researcher : Teng LL

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Mok M.Y., Teng L.L., Tam V.K., Chan K.K. and Yuen K.Y., Characterization of Haemophilus segnis, an important cause of bacteremia, by 16S rRNA gene sequencing, Journal of Clinical Microbiology . 2004, 42: 877-80.

Lau S.K.P., Woo P.C.Y., Hui W.T., Li M.W., Teng L.L., Que T.L., Luk W.K., Lai R.W., Yung R.W. and Yuen K.Y., Use of cefoperazone MacConkey agar for selective isolation of Laribacter hongkongensis, Journal of Clinical Microbiology . 2003, 41: 4839-41.

Woo P.C.Y., Fung A.S.M., Lau S.K.P., Teng L.L., Wong B.H., Wong M.K., Hon E.H.C., Tang G.W.K. and Yuen K.Y., Actinomyces hongkongensis sp. nov. a novel Actinomyces species isolated from a patient with pelvic actinomycosis, Systematic and Applied Microbiology . 2003, 26(4): 518-22.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Woo P.C.Y., Kuhnert P., Burnens A.P., Teng L.L., Lau S.K.P., Que T.L., Yau H.H. and Yuen K.Y., Laribacter hongkongensis: a potential cause of infectious diarrhea, Diagnostic Microbiology and Infectious Disease . 2003, 47: 551-6.

Woo P.C.Y., Zhen H., Cai J.J., Yu J., Lau S.K.P., Wang J., Teng L.L., Wong S.S.Y., Tse R.H., Chen R., Yang H., Liu B. and Yuen K.Y., The mitochondrial genome of the thermal dimorphic fungus Penicillium marneffei is more closely related to those of molds than yeasts, FEBS Letters . 2003, 555: 469-77.

Researcher : To APC

List of Research Outputs

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Lau S.K.P., Woo P.C.Y., To A.P.C., Wong T.K. and Yuen K.Y., Lack of Evidence for DDNA in antibiotic preparations as source of antibiotic resistance genes, 14th European Congress of Clinical Microbiology and infectious Diseases. Prague, The Czech Republic.. 2004.

Lau S.K.P., Woo P.C.Y., Yim T.C., To A.P.C. and Yuen K.Y., Molecular characterization of a strain of ngroup a streptococcus isolated from a patient with a psoas abscess, Journal of Clinical Microbiology . 2003, 41: 4888-91.

Lee R.A., Woo P.C.Y., To A.P.C., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Geographical difference of disease association in Streptococcus bovis bacteraemia, Journal of Medical Microbiology . 2003, 52: 903-8.

Woo P.C.Y., To A.P.C., Tse H., Lau S.K.P. and Yuen K.Y., Clinical and molecular epidemiology of erythromycin-resistant beta-hemolytic lancefield group G streptococci causing bacteremia, Journal of Clinical Microbiology . 2003, 41: 5188-91.

Woo P.C.Y., To A.P.C., Lau S.K.P. and Yuen K.Y., Facilitation of horizontal transfer of antimicrobial resistance by transformation of antibiotic-induced cell-wall-deficient bacteria, Medical Hypotheses . 2003, 61: 503-8.

Researcher : Tong FH

List of Research Outputs

Gu H., Ho P.L., Tong F.H., Xu C. and Xu B., Presenting vancomycin on nanoparticles to enhance antimicrobial activities, Nano Letters. 2003, 3(9): 1261-3.

Researcher : Tsoi HW

List of Research Outputs

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Chan K.H., Tam V.K., Peiris J.S.M. and Yuen K.Y., Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnsosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2004, 42(5): 2306-9.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Researcher : Tsui WHW

List of Research Outputs

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Researcher : Wang J

List of Research Outputs

Woo P.C.Y., Zhen H., Cai J.J., Yu J., Lau S.K.P., Wang J., Teng L.L., Wong S.S.Y., Tse R.H., Chen R., Yang H., Liu B. and Yuen K.Y., The mitochondrial genome of the thermal dimorphic fungus Penicillium marneffei is more closely related to those of molds than yeasts, FEBS Letters . 2003, 555: 469-77.

Researcher : Wang TKF

List of Research Outputs

Wang T.K.F., Chiu W., Chim S., Chan D.T.M. and Wong S.S.Y., Disseminated ochroconis gallopavum infection in a renal transplant recipient: the first reported case and a review of the literature, Clinical Nephrology. 2003, 2003; 60(6): 415-23.

Wong S.S.Y., Woo P.C.Y., Ho P.L. and Wang T.K.F., Continuous ambulatory peritoneal dialysis-related peritonitis caused by Streptococcus bovis. , Eur J Clin Microbiol Infect Dis.. 2003, 22(7): 424-6.

Researcher : Webster RG

List of Research Outputs

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Peiris J.S.M., Poon L.L.M., Dyrting K.C., Ellis T.M., Sims L., Webster R.G. and Shortridge K.F., Reassortants of H5N1 influenza viruses recently isolated from aquatic poultry in Hong Kong SAR., Avian Diseases. 911-3, 2003, 47(3 Suppl).

Liu M., Guan Y., Peiris J.S.M., He S., Webby R.J., Perez D. and Webster R.G., The quest of influenza A viruses for new hosts, Avian Diseases. 2003, 47(3 Suppl): 849-56.

Sturm-Ramirez K.M., Ellis T., Bousfield B., Guan Y., Peiris J.S.M. and Webster R.G., H5N1 influenza A viruses from 2002 are highly pathogenic in waterfowl, Options for the Control of Influenza V. 2004, International Congress Series 1263: 200-204.

Sturm-Ramirez K.M., Ellis T., Bousfield B., Bissett L., Dyrting K., Rehg J.E., Poon L.L.M., Guan Y., Peiris J.S.M. and Webster R.G., Reemerging H5N1 influenza viruses in Hong Kong in 2002 are highly pathogenic to ducks., Journal of Virology. 2004, 78(9): 4892-901.

Webby R.J., Perez D.R., Coleman J.S., Guan Y., Knight J.H., Govorkova E.A., McClain-Moss L.R., Peiris J.S.M., Rehg J.E., Tuomanen E.I. and Webster R.G., Responsiveness to a pandemic alert: use of reverse genetics for rapid development of influenza vaccines., The Lancet. 2004, 363(9415): 1099-103.

Researcher : Wong BCW

List of Research Outputs

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Researcher : Wong BHL

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Chan K.H., Tam V.K., Peiris J.S.M. and Yuen K.Y., Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnsosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2004, 42(5): 2306-9.

Researcher : Wong KL

List of Research Outputs

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Zheng B., Guan Y., Tang Q., Cheng D., Xie F.Y., He M.L., Chan K.W., Wong K.L., Lader E., Woodle M.C., Lu P.Y., Li B. and Zhong N., Prophylactic and therapeutic effects of small interfering RNA targeting SARS-coronavirus, Antiviral Therapy. 2004, 9: 365-374.

Zheng B., Guan Y., Wong K.H., Zhou J., Wong K.L., Young B.W.Y., Lu L. and Lee S.S., SARS-related virus predating SARS outbreak, Hong Kong, Emerging Infectious Diseases. 2004, 10(2): 176-178.

Researcher : Wong LP

List of Research Outputs

Chu C.M., Cheng V.C.C., Hung I.F.N., Wong M.M., Chan K.H., Chan K.S., Kao R.Y.T., Poon L.L.M., Wong L.P., Guan Y. and Peiris J.S.M., Role of lopinavir/ritonavir in the treatment of SARS: initial virological and clinical findings., Thorax. 2004, 59(3): 252-6.

Researcher : Wong MKM

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Woo G.K.S., Fung A.M.Y., Wong M.K.M., Chan K.M., Tam D.M.W. and Yuen K.Y., Eggerthella hongkongensis sp. nov. and Eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia, Diagnostic Microbiology and Infectious Disease. Elsevier, 2004, 49: 255-263.

Researcher : Wong OK

List of Research Outputs

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Poon L.L.M., Chan K.H., Wong O.K., Yam W.C., Yuen K.Y., Guan Y., Lo Y.M. .D.E.N.N.I.S. and Peiris J.S.M., Early diagnosis of SARS Coronavirus infection by real time RT-PCR, Journal of Clinical Virology. 2003, 28(3): 233-8.

Poon L.L.M., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S.M. and Guan Y., Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). , Clin Chem. 2003, 49: 953-5.

Researcher : Wong SSY

Project Title:	Random exploration of the genome of the dimorphic fungus penicillium marneffei
Investigator(s):	Dr. Wong S.S.Y., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001
Completion Date:	10/2003

Abstract:

To answer the following questions: (1) the number and sizes of the chromosomes (karyotyping) of P. marneffei; (2) the phylogenetic relationship between P. marneffei and other fungi; specially, whether a sexual stage of it exists in nature which explains why it is difficult to isolate the fungus from environmental samples; (3) to perform a random exploration of the P. marneffei genome and analyze 2000 random sequence tags of the genome of the fungus. This would enable us to better understand the basic mechanisms of dimorphism, biochemical properties, disease pathogenesis, virulence, and immune defence and thus pave the way to treat or prevent the disease.

Project Title:	A novel method of bacterial identification: groEL gene sequencing for discriminating Burkholderia pseudomallei and Burkholderia thailandensis
Investigator(s):	Dr. Wong S.S.Y., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To clone the groEL gene of B. thailandensis; to examine the potential of using this gene target for discriminating between B. thailandensis and B. pseudomallei.

Project Title:	Molecular characterization of streptococcus bovis bacteraemia
Investigator(s):	Dr. Wong S.S.Y., Dr. Woo P.C.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To characterize 48 S. bovis strains isolated from the blood cultures of 37 bacteraemic patients over a six-year period using a combination of phenotypic and genotypic techniques. The epidemiology, underlying diseases, clinical disease associations, and outcome of S. bovis bacteraemia in relation to the different biotypes and genotypes of S. bovis will be investigated, and the molecular epidemiology of erythromycin resistance in the S. bovis strains will also be analyzed.

Project Title:	Screening antigenic proteins in penicillium marneffei for seroepidemiology and vaccination
Investigator(s):	Dr. Wong S.S.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	12/2003

Abstract:

To screen for yeast- and mould-phase-specific antigens that are useful for epidemiological and diagnostic purposes; to screen for yeast-phase- and mould-phase-specific antigens that induce active immunity against penicilliosis marneffei from normalized cDNA libraries of the two phases of the dimorphic fungus; to construct a prophylactic and/or therapeutic vaccine prototype; to study the seroepidemiology of penicilliosis marneffei.

Project Title:	Screening antigenic proteins in penicillium marneffei for seroepidemiology and vaccination
Investigator(s):	Dr. Wong S.S.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	12/2003

Abstract:

List of Research Outputs

Ho P.L., Wong R.C., Chow K.H., Cheung M.Y., Wong S.S.Y., Yam W.C. and Que T.L., Application of a multiplex pbp2b and pbp2x PCR for prediction of penicillin resistance in Streptococcus pneumoniae., Journal of Antimicrobial Chemotherapy. 2004, 53(5): 890-1.

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distrubtion and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong Children, Vaccine. Elsevier Ltd, 2004, 22: 3334-3339.

Lee R.A., Woo P.C.Y., To A.P.C., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Geographical difference of disease association in Streptococcus bovis bacteraemia, Journal of Medical Microbiology . 2003, 52: 903-8.

Tse K.C., Chan D.T.M., Wong S.S.Y., Chan K.W., Lam M.F., Chan K.H.N., Li F.K., Chung B.C.Y. and Lai K.N., Granulomatous interstitial nephritis of the allograft kidney associated with rhodococcal pulmonary infection. Nephrol Dial Transplant, Nephrology Dialysis Transplantation. 2004, 19: 486-490.

Wang T.K.F., Chiu W., Chim S., Chan D.T.M. and Wong S.S.Y., Disseminated ochroconis gallopavum infection in a renal transplant recipient: the first reported case and a review of the literature, Clinical Nephrology. 2003, 2003; 60(6): 415-23.

Wong S.S.Y., Woo P.C.Y., Ho P.L. and Wang T.K.F., Continuous ambulatory peritoneal dialysis-related peritonitis caused by Streptococcus bovis. , Eur J Clin Microbiol Infect Dis.. 2003, 22(7): 424-6.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Woo P.C.Y., Zhen H., Cai J.J., Yu J., Lau S.K.P., Wang J., Teng L.L., Wong S.S.Y., Tse R.H., Chen R., Yang H., Liu B. and Yuen K.Y., The mitochondrial genome of the thermal dimorphic fungus Penicillium marneffei is more closely related to those of molds than yeasts, FEBS Letters . 2003, 555: 469-77.

Woo P.C.Y., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Two cases of continuous ambulatory peritoneal dialysis-associated peritonitis due to Plesiomonas shigelloides, Journal of Clinical Microbiology . 2004, 42: 933-5.

Researcher : Woo GKS

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Lau S.K.P., Woo P.C.Y., Woo G.K.S., Fung A.M.Y., Wong M.K.M., Chan K.M., Tam D.M.W. and Yuen K.Y., Eggerthella hongkongensis sp. nov. and Eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia, Diagnostic Microbiology and Infectious Disease. Elsevier, 2004, 49: 255-263.

Researcher : Woo PCY

Project Title:	Penicilliosis marneffei in AIDS patients
Investigator(s):	Dr. Woo P.C.Y., Dr. Chan K., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund
Start Date:	07/2001

Abstract:

To search for a novel antigen of which the immune response against it is life long, such antigen would be crucial in the understanding of the seroepidemiology of the infection, as well as how the pathogen is transmitted exactly. Furthermore, such an antigen may be important in the development of vaccine in penicilliosis marneffei. This could be important to reduce the need for secondary prophylaxis as these patients may have to take antifungal for life.

Project Title:	Molecular characterization of thermotolerant Campylobacter species isolated from bacteremic patients
Investigator(s):	Dr. Woo P.C.Y., Dr. Wong S.S.Y., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001
Completion Date:	10/2003

Abstract:

To study clinical characteristics of the corresponding patients

Project Title:	A novel gold standard of bacterial identification: granulicatella adiacens and abiotrophia defectiva bacteremia characterized by 16S ribosomal RNA gene sequencing
Investigator(s):	Dr. Woo P.C.Y., Dr. Lau S.K.P., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To use 16S rRNA gene sequencing, assisted by traditional phenotypic tests; to define the epidemiology, clinical diseases, and outcome of G. adiacens and A. defectiva bacteremia.

Project Title:	Development of an ELISA-based antibody test for SARS associated coronavirus infection
Investigator(s):	Dr. Woo P.C.Y., Dr. Lau S.K.P., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To develop and evaluate an ELISA based on the highly immunogenic nucleocapsid protein of SARS associated coronavirus; to examine the seroprevalence of the general population before and after the SARS outbreak and non-pneumonic SARS associated coronavirus infections in both the general population and health care workers.

Project Title:	Epidemiology and pathogenesis of a novel clinical syndrome: laribacter hongkongensis gastroenteritis
Investigator(s):	Dr. Woo P.C.Y., Dr. Lau S.K.P., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To study the epidemiology of L. hongkongensis gastroenteritis in humans; to provide clinicians with appropriate treatment regimen by correlation of antibiotic susceptibilities and clinical outcome; to discover the source and reservoir of the bacterium; to define its pathogenesis in terms of toxin production, tissue and animal invasiveness.

Project Title:	Molecular epidemiology of erythromycin resistant b-hemolytic Lancefield group G streptococci causing bacteremia
Investigator(s):	Dr. Woo P.C.Y., Dr. Lau S.K.P., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To perform phenotypic and genotypic characterization of the erythromycin resistance in b-hemolytic group G streptococci recovered from blood cultures of patients in a six-year period. Risk factors for bacteremia caused by b-hemolytic group G streptocci will also be analyzed.

List of Research Outputs

Cheng V.C.C., Yam W.C., Hung I.F., Woo P.C.Y., Lau S.K.P., Tang S.F. and Yuen K.Y., Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis, Journal of Clinical Pathology . 2004, 57: 281-5.

Cheng V.C.C., Yam W.C., Woo P.C.Y., Lau S.K.P., Hung I.F., Wong S.P., Cheung W.C. and Yuen K.Y., Risk Factors for Development of Paradoxical Response During Antituberculosis Therapy in HIV-Negative Patients, Eur J Clin Microbiol Infect Dis. 2003, 22: 597-602.

Chong K.T., Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., AFMP2 Encodes a Novel Immunogenic Protein of the Antigenic Mannoprotein Superfamily in Aspergillus fumigatus. , Journal of Clinical Microbiology . 2004, 42: 2287-91.

Chu C.M., Woo P.C.Y., Chong K.T., Leung W.S., Chan V.L. and Yuen K.Y., Association of presence of Aspergillus antibodies with hemoptysis in patients with old tuberculosis or bronchiectasis but no radiologically visible mycetoma, Journal of Clinical Microbiology . 2004, 42: 665-9.

Lau S.K.P., Woo P.C.Y., Mok M.Y., Teng L.L., Tam V.K., Chan K.K. and Yuen K.Y., Characterization of Haemophilus segnis, an important cause of bacteremia, by 16S rRNA gene sequencing, Journal of Clinical Microbiology . 2004, 42: 877-80.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Lau S.K.P., Woo P.C.Y., Woo G.K.S., Fung A.M.Y., Wong M.K.M., Chan K.M., Tam D.M.W. and Yuen K.Y., Eggerthella hongkongensis sp. nov. and Eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia, Diagnostic Microbiology and Infectious Disease. Elsevier, 2004, 49: 255-263.

Lau S.K.P., Woo P.C.Y., To A.P.C., Wong T.K. and Yuen K.Y., Lack of Evidence for DDNA in antibiotic preparations as source of antibiotic resistance genes, 14th European Congress of Clinical Microbiology and infectious Diseases. Prague, The Czech Republic.. 2004.

Lau S.K.P., Woo P.C.Y., Yim T.C., To A.P.C. and Yuen K.Y., Molecular characterization of a strain of ngroup a streptococcus isolated from a patient with a psoas abscess, Journal of Clinical Microbiology . 2003, 41: 4888-91.

Lau S.K.P., Woo P.C.Y., Hui W.T., Li M.W., Teng L.L., Que T.L., Luk W.K., Lai R.W., Yung R.W. and Yuen K.Y., Use of cefoperazone MacConkey agar for selective isolation of Laribacter hongkongensis, Journal of Clinical Microbiology . 2003, 41: 4839-41.

Lee R.A., Woo P.C.Y., To A.P.C., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Geographical difference of disease association in Streptococcus bovis bacteraemia, Journal of Medical Microbiology . 2003, 52: 903-8.

Wong S.S.Y., Woo P.C.Y., Ho P.L. and Wang T.K.F., Continuous ambulatory peritoneal dialysis-related peritonitis caused by Streptococcus bovis. , Eur J Clin Microbiol Infect Dis.. 2003, 22(7): 424-6.

Woo P.C.Y., Fung A.S.M., Lau S.K.P., Teng L.L., Wong B.H., Wong M.K., Hon E.H.C., Tang G.W.K. and Yuen K.Y., Actinomyces hongkongensis sp. nov. a novel Actinomyces species isolated from a patient with pelvic actinomycosis, Systematic and Applied Microbiology . 2003, 26(4): 518-22.

Woo P.C.Y., To A.P.C., Tse H., Lau S.K.P. and Yuen K.Y., Clinical and molecular epidemiology of erythromycin-resistant beta-hemolytic lancefield group G streptococci causing bacteremia, Journal of Clinical Microbiology . 2003, 41: 5188-91.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Chan K.H., Tam V.K., Peiris J.S.M. and Yuen K.Y., Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnsosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2004, 42(5): 2306-9.

Woo P.C.Y., Tam D.M., Lau S.K.P., Fung A.M. and Yuen K.Y., Enterococcus cecorum empyema thoracis successfully treated with cefotaxime, Journal of Clinical Microbiology . 2004, 42: 919-22.

Woo P.C.Y., To A.P.C., Lau S.K.P. and Yuen K.Y., Facilitation of horizontal transfer of antimicrobial resistance by transformation of antibiotic-induced cell-wall-deficient bacteria, Medical Hypotheses . 2003, 61: 503-8.

Woo P.C.Y., Lau S.K.P., Fung A.M., Chiu S.K., Yung R.W. and Yuen K.Y., Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology . 2003, 56: 690-3.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Woo P.C.Y., Kuhnert P., Burnens A.P., Teng L.L., Lau S.K.P., Que T.L., Yau H.H. and Yuen K.Y., Laribacter hongkongensis: a potential cause of infectious diarrhea, Diagnostic Microbiology and Infectious Disease . 2003, 47: 551-6.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Woo P.C.Y., Tse H., Chan K.M., Lau S.K.P., Fung A.M., Yip K.T., Tam D.M., Ng K.H., Que T.L. and Yuen K.Y., Streptococcus milleri" endocarditis caused by Streptococcus anginosus, Diagnostic Microbiology and Infectious Disease . 2004, 48: 81-8.

Woo P.C.Y., Zhen H., Cai J.J., Yu J., Lau S.K.P., Wang J., Teng L.L., Wong S.S.Y., Tse R.H., Chen R., Yang H., Liu B. and Yuen K.Y., The mitochondrial genome of the thermal dimorphic fungus Penicillium marneffei is more closely related to those of molds than yeasts, FEBS Letters . 2003, 555: 469-77.

Woo P.C.Y., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Two cases of continuous ambulatory peritoneal dialysis-associated peritonitis due to Plesiomonas shigelloides, Journal of Clinical Microbiology . 2004, 42: 933-5.

Researcher : Yam WC

Project Title:	Collaborative study of individual and pooled NAT for Hepatitis B virus in donated blood
Investigator(s):	Dr. Yam W.C.
Department:	Microbiology (Hospital Pathology Services)
Source(s) of Funding:	Hong Kong Red Cross
Start Date:	11/2003

Abstract:

To carry out collaborative study of individual and pooled NAT for Hepatitis B virus in donated blood.

List of Research Outputs

Cheng V.C.C., Yam W.C., Hung I.F., Woo P.C.Y., Lau S.K.P., Tang S.F. and Yuen K.Y., Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis, Journal of Clinical Pathology . 2004, 57: 281-5.

Cheng V.C.C., Yam W.C., Woo P.C.Y., Lau S.K.P., Hung I.F., Wong S.P., Cheung W.C. and Yuen K.Y., Risk Factors for Development of Paradoxical Response During Antituberculosis Therapy in HIV-Negative Patients, Eur J Clin Microbiol Infect Dis. 2003, 22: 597-602.

Ho P.L., Wong R.C., Chow K.H., Cheung M.Y., Wong S.S.Y., Yam W.C. and Que T.L., Application of a multiplex pbp2b and pbp2x PCR for prediction of penicillin resistance in Streptococcus pneumoniae., Journal of Antimicrobial Chemotherapy. 2004, 53(5): 890-1.

Leung E.T.Y., Kam K.M., Chiu A., Ho P.L., Seto W.H., Yuen K.Y. and Yam W.C., Detection of katG Ser315Thr substitution in respiratory specimens from patients with isoniazid-resistant Mycobacterium tuberculosis using PCR-RFLP, Journal of Medical Microbiology. 2003, 52(Pt 11): 999-1003.

Leung P.H., Peiris J.S.M., Ng W.W.S., Robins-Browne R.M., Bettelheim K.A. and Yam W.C., A newly discovered verotoxin variant, VT2g, produced by bovine verocytotoxigenic Escherichia coli., Applied and Environmental Microbiology. 2003, 69(12): 7549-53.

Poon L.L.M., Chan K.H., Wong O.K., Yam W.C., Yuen K.Y., Guan Y., Lo Y.M. .D.E.N.N.I.S. and Peiris J.S.M., Early diagnosis of SARS Coronavirus infection by real time RT-PCR, Journal of Clinical Virology. 2003, 28(3): 233-8.

Yam W.C., Cheng V.C.C., Hui W.T., Wang L.N., Seto W.H. and Yuen K.Y., Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA), Diagnostic Microbiology and Infectious Disease . 2004, 48: 271-5.

Yam W.C., Chan K.H., Poon L.L.M., Guan Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus, Journal of Clinical Microbiology. 2003, 41(10): 4521-4524.

Researcher : Yap YL

List of Research Outputs

Yap Y.L., Zhang X. and Danchin A.L.M., Relationship of SARS-CoV to Other Pathogenic RNA Viruses explored by tetranucleotide usage profiling, BMC Bioinformatics. 2003, 4(1): 43.

Zhang X. and Yap Y.L., Exploring the binding mechanism of the main proteinase in SARS-associated coronavirus and its implication to anti-SARS drug design, Bioorganic & Medicinal Chemistry. 2004, 12 (9): 2219-23.

Zhang X. and Yap Y.L., Old drugs as lead compounds for a new disease? Binding analysis of SARS coronavirus main proteinase with HIV, psychotic and parasite drugs, Bioorganic & Medicinal Chemistry. 2004, 12(10): 2517-2521.

Zhang X. and Yap Y.L., Structural similarity between HIV-1 gp41 and SARS-CoV S2 proteins suggests an analogous membrane fusion mechanism, Journal of Molecular Structure, THEOCHEM. 2004, 677: 73-76.

Researcher : Yuen KY

Project Title:	Characterization of a novel insertion sequence, ISBp1, in Burkholderia pseudomallei
Investigator(s):	Prof. Yuen K.Y., Dr. Wong S.S.Y., Dr. Woo P.C.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2001
Completion Date:	10/2003

Abstract:

To clone and characterize a novel insertion sequence in B. pseudomallei. The potential usefulness of such a sequence for molecular typing will also be investigated.

Project Title:	Establishment of core facilities for the HKU HIV/AIDS research laboratories
Investigator(s):	Prof. Yuen K.Y., Dr. Im S.W.K., Dr. Woo P.C.Y., Dr. Yam W.C., Dr. Zheng B.
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund
Start Date:	11/2001

Abstract:

To support the development of state-of-art clinical management of HIV/AIDS founded on scientific research and clinical trials; to undertake research for expanding the knowledge base on HIV molecular epidemiology in Hong Kong and the reigon; to examine the immunological mechanisms underlying HIV pathogenesis; to study AIDS associated infectious diseases; to build expertise in HIV molecular biology and immunology.

Project Title:	Molecular characterization of a novel type of "Viridans Streptococcus"
Investigator(s):	Prof. Yuen K.Y., Dr. Lau S.K.P., Dr. Woo P.C.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2002

Abstract:

To report the isolation of a catalase negative, a-hemolytic, optochin and bacitracin resistant "viridans streptococcus" that does not grow in 10% or 40% bile, on MacConkey agar or bile esculin agar, or in 6% NaC1, from the blood culture of a 73-year old woman with pyomyositis and post-streptococcal reactive arthritis.

Project Title:	An oral DNA vaccine for SARS associated coronavirus infections
Investigator(s):	Prof. Yuen K.Y., Dr. Lau S.K.P., Dr. Woo P.C.Y.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To perform a pilot study to test the feasibility of using the live-attenuated Salmonella system for delivery of DNA vaccine of the nucleocapsid protein of SARS associated coronavirus in mice, and compare the immunogenicity of oral DNA vaccine, naked DNA vaccine, and recombinant protein vaccine.

Project Title:	Molecular characterization of Streptococcus milleri causing infective endocarditis
Investigator(s):	Prof. Yuen K.Y., Dr. Lau S.K.P., Dr. Woo P.C.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	11/2003

Abstract:

To identify members of the "S. milleri group" in patients with infective endocarditis by 16S rRNA gene sequencing; to define the epidemiology, clinical spectrum, treatment, and outcome of infective endocarditis associated with the "S. milleri group".

List of Research Outputs

Au W.Y., Cheng V.C.C., Ho P.L., Yuen K.Y., Hung I., Ma S.Y., Lie A.K.W., Liang R.H.S. and Kwong Y.L., Non tuberculous mycobacterial infections in Chinese hematopoietic stem cell transplantation recipients., Bone Marrow Transplantation. 2003, 32: 709-714.

Chan K.H., Poon L.L.M., Cheng V.C.C., Guan Y., Hung I.F.N., Kong J., Yam L.Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with suspected SARS., Emerging Infectious Diseases. 2004, 10(2): 294-9.

Chan K.S., Lai S.T., Chu C.M., Tsui E., Tam C.Y., Wong M.M., Tse M.W., Que T.L., Peiris J.S.M., Sung J., Wong V.C. and Yuen K.Y., Treatment of severe acute respiratory syndrome with lopinavir/ritonavir: a multicentre retrospective matched cohort study., Hong Kong Medical Journal. 2003, 9(6): 399-406.

Che X.Y., Qiu L.W., Pan Y.X., Wen K., Hao W., Zhang L.Y., Wang Y.D., Liao Z.Y., Hua X., Cheng V.C.C. and Yuen K.Y., Sensitive and specific monoclonal antibody-based capture enzyme immunoassay for detection of nucleocapsid antigen in sera from patients with severe acute respiratory syndrome. , Journal of Clinical Microbiology. 2004, 42(6): 2629-35.

Cheng V.C.C., Yam W.C., Hung I.F., Woo P.C.Y., Lau S.K.P., Tang S.F. and Yuen K.Y., Clinical evaluation of the polymerase chain reaction for the rapid diagnosis of tuberculosis, Journal of Clinical Pathology . 2004, 57: 281-5.

Cheng V.C.C., Hung I.F.N., Wu A.K., Tang S.F., Chu C.M. and Yuen K.Y., Lymphocyte surge as a marker for immunorestitution disease due to Pneumocystis jiroveci pneumonia in HIV-negative immunosuppressed hosts., European journal of clinical microbiology & infectious diseases. 2004, 23(6): 512-4.

Cheng V.C.C., Yam W.C., Woo P.C.Y., Lau S.K.P., Hung I.F., Wong S.P., Cheung W.C. and Yuen K.Y., Risk Factors for Development of Paradoxical Response During Antituberculosis Therapy in HIV-Negative Patients, Eur J Clin Microbiol Infect Dis. 2003, 22: 597-602.

Cheng V.C.C., Hung I.F.N., Tang B.S., Chu C.M., Wong M.M., Chan K.H., Wu A.K., Wu A.K., Tse D.M., Chan K.S., Zheng B., Peiris J.S.M., Sung J.J. and Yuen K.Y., Viral replication in the nasopharynx is associated with diarrhea in patients with severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(4): 467-75.

Chim S.S., Tsui S.K., Chan K.C., Au T.C., Hung E.C., Tong Y.K., Chiu R.W., Ng E.K., Chan P.K., Chu C.M., Sung J.J., Tam J.S., Fung K.P., Waye M.M., Lee C.Y., Yuen K.Y. and Lo Y.M., CUHK Molecular SARS Research Group. Genomic characterisation of the severe acute respiratory syndrome coronavirus of Amoy Gardens outbreak in Hong Kong, Lancet. 2003, 362: 1807-8.

Chong K.T., Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., AFMP2 Encodes a Novel Immunogenic Protein of the Antigenic Mannoprotein Superfamily in Aspergillus fumigatus. , Journal of Clinical Microbiology . 2004, 42: 2287-91.

Chow L.W., Loo W.T., Yuen K.Y. and Cheng C., The study of cytokine dynamics at the operation site after mastectomy, Wound Repair Regen. 2003, 11: 326-30.

Chu C.M., Woo P.C.Y., Chong K.T., Leung W.S., Chan V.L. and Yuen K.Y., Association of presence of Aspergillus antibodies with hemoptysis in patients with old tuberculosis or bronchiectasis but no radiologically visible mycetoma, Journal of Clinical Microbiology . 2004, 42: 665-9.

Chu C.M., Leung Y.Y., Hui J.Y., Hung I.F.N., Chan V.L., Leung W.S., Law K.I., Chan C.S., Chan K.S. and Yuen K.Y., Spontaneous pneumomediastinum in patients with severe acute respiratory syndrome, The European respiratory journal. 2004, 23(6): 802-4.

Guan Y., Poon L.L.M., Cheung C.Y., Ellis T.M., Lim W.W.L., Lipatov A.S., Chan K.H., Sturm-Ramirez K.M., Cheung C.L., Leung C.Y.H., Yuen K.Y., Webster R.G. and Peiris J.S.M., H5N1 influenza: A protein pandemic threat, Proceedings of National Academic Science (USA). 2004, 101(21): 8156-8161.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Guan Y., Peiris J.S.M., Zheng B., Poon L.L.M., Chan K.H., Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Leung C.S.W., Yuen K.Y. and Leung F.C.C., Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome, The Lancet. 2004, 363: 99-104.

He M.L., Zheng B., Peng Y., Peiris J.S., Poon L.L., Yuen K.Y., Lin M.C., Kung H. and Guan Y., Inhibition of SARS-associated coronavirus infection and replication by RNA interference, Journal of the American Medical Association. 2003, 290(20): 2665-6.

Hui K.H., Zeng F., Chan M.N., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., RT-PCR Diagnosis of Coronavirus Associated with Severe Acute Respiratory Syndrome (SARS), Journal Clinical Microbiology. 2004, 42: 1994-1999.

Hui R.K., Zeng F., Chan C.M., Yuen K.Y., Peiris J.S.M. and Leung F.C., Reverse transcriptase PCR diagnostic assay for the coronavirus associated with severe acute respiratory syndrome, Journal of Clinical Microbiology . 2004, 42: 1994-9.

Kao R.Y.T., Tsui W.H.W., Lee T.S.W., Tanner J.A., Watt R.M., Huang J., Hu L., Chen G., Chen Z., Zhang L., He T., Chan K.H., Tse H., To A.P.C., Ng L.W.Y., Wong B.C.W., Tsoi H.W., Yang D., Ho D.D. and Yuen K.Y., Identification of Novel Small-Molecule Inhibitors of Severe Acute Respiratory Syndrome-Associated Coronavirus by Chemical Genetics, Chemistry and Biology. 2004, V11(9): 1293-1299.

Lau K.K., Yu W.C., Chu C.M., Lau S.T., Sheng B. and Yuen K.Y., Possible central nervous system infection by SARS coronavirus, Emerging Infectious Diseases . 2004, 10: 342-4.

Lau S.K.P., Woo P.C.Y., Mok M.Y., Teng L.L., Tam V.K., Chan K.K. and Yuen K.Y., Characterization of Haemophilus segnis, an important cause of bacteremia, by 16S rRNA gene sequencing, Journal of Clinical Microbiology . 2004, 42: 877-80.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in SARS patients by enzyme-linked immunosorbent assay, Journal of Clinical Microbiology. 2004, 42(7): 2884-2889.

Lau S.K.P., Woo P.C.Y., Woo G.K.S., Fung A.M.Y., Wong M.K.M., Chan K.M., Tam D.M.W. and Yuen K.Y., Eggerthella hongkongensis sp. nov. and Eggerthella sinensis sp. nov., two novel Eggerthella species, account for half of the cases of Eggerthella bacteremia, Diagnostic Microbiology and Infectious Disease. Elsevier, 2004, 49: 255-263.

Lau S.K.P., Woo P.C.Y., To A.P.C., Wong T.K. and Yuen K.Y., Lack of Evidence for DDNA in antibiotic preparations as source of antibiotic resistance genes, 14th European Congress of Clinical Microbiology and infectious Diseases. Prague, The Czech Republic.. 2004.

Lau S.K.P., Woo P.C.Y., Yim T.C., To A.P.C. and Yuen K.Y., Molecular characterization of a strain of ngroup a streptococcus isolated from a patient with a psoas abscess, Journal of Clinical Microbiology . 2003, 41: 4888-91.

Lau S.K.P., Woo P.C.Y., Hui W.T., Li M.W., Teng L.L., Que T.L., Luk W.K., Lai R.W., Yung R.W. and Yuen K.Y., Use of cefoperazone MacConkey agar for selective isolation of Laribacter hongkongensis, Journal of Clinical Microbiology . 2003, 41: 4839-41.

Lee R.A., Woo P.C.Y., To A.P.C., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Geographical difference of disease association in Streptococcus bovis bacteraemia, Journal of Medical Microbiology . 2003, 52: 903-8.

Leung E.T.Y., Kam K.M., Chiu A., Ho P.L., Seto W.H., Yuen K.Y. and Yam W.C., Detection of katG Ser315Thr substitution in respiratory specimens from patients with isoniazid-resistant Mycobacterium tuberculosis using PCR-RFLP, Journal of Medical Microbiology. 2003, 52(Pt 11): 999-1003.

Leung W.K., To K.F., Chan P.K., Chan H.L., Wu A.K., Lee N., Yuen K.Y. and Sung J.J., Enteric involvement of severe acute respiratory syndrome-associated coronavirus infection, Gastroenterology. 2003, 125: 1011-7.

Ooi C.G.C., Ahuja A., Wong S.W., Sy N.L., Hui J., Chan Y.F., Yuen K.Y., Huang H. and Leong Fung L.L.Y., Radiology of the chest in SARS., Hong Kong SARS Forum and Hospital Authority Convention, Hong Kong. 2004.

Peiris J.S.M., Yu W.C., Leung C.W., Cheung C.Y., Ng W.F., Nicholls J.M., Ng T.K., Chan K.H., Lai S.T., Lim W.W.L., Yuen K.Y. and Guan Y., Re-emergence of fatal human influenza A subtype H5N1 disease, Lancet. 2004, 363(9409): 617-619.

Peiris J.S.M., Yuen K.Y., Osterhaus A.D. and Stohr K., The severe acute respiratory syndrome., The New England Journal of Medicine. 2003, 349(25): 2431-41.

Poon L.L.M., Chan K.H., Nicholls J.M., Zheng B., Yuen K.Y., Guan Y. and Peiris J.S.M., Characterization of a novel coronavirus responsible for severe acute respiratory syndrome, Options for the Control of Influenza V. 2004, International Congress Series 1263: 805-808.

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Poon L.L.M., Chan K.H., Wong O.K., Yam W.C., Yuen K.Y., Guan Y., Lo Y.M. .D.E.N.N.I.S. and Peiris J.S.M., Early diagnosis of SARS Coronavirus infection by real time RT-PCR, Journal of Clinical Virology. 2003, 28(3): 233-8.

Poon L.L.M., Leung C.S., Tashiro M., Chan K.H., Wong B.W., Yuen K.Y., Guan Y. and Peiris J.S.M., Rapid Detection of the Severe Acute Respiratory Syndrome (SARS) Coronavirus by a Loop-Mediated Isothermal Amplification Assay, Clinical Chemistry . 2004, 50: 1050-52.

Poon L.L.M., Wong O.K., Chan K.H., Luk W., Yuen K.Y., Peiris J.S.M. and Guan Y., Rapid diagnosis of a coronavirus associated with severe acute respiratory syndrome (SARS). , Clin Chem. 2003, 49: 953-5.

Sung J.J., Wu A., Joynt G.M., Yuen K.Y., Lee N., Chan P.K., Cockrams C.S., Ahuja A.T., Yu L.M., Wong V.W. and Hui D.S., Severe acute respiratory syndrome: report of treatment and outcome after a major outbreak, Thorax. 2004, 59: 414-20.

Tsang K.W.T., Tipoe G.L., Sun J.Z., Leung Y.H., Ho P.L., Yuen K.Y., Mak J.C.W. and Lam W.K., Helicobacter pylori exotoxin disrupts ciliary beating and ultrastructure of human airway mucosa, 8th Asian Pacific Society of Respirology Congress, Kuala Lumpur, Malaysia. 2003, Abstract Book PP6-22: p.201.

Wen K., Mei Y.B., Qiu L.W., Liao Z.Y., Yuen K.Y. and Che X.Y., Preparation and characterization of monoclonal antibodies against S1 domain at N-terminal residues 249 to 667 of SARS-associated coronavirus S1 protein, Di Yi Jun Yi Da Xue Xue Bao. 2004, 24: 1-6.

Wong A.T., Tsang O.T., Wong M.Y., Lim W.L., Zheng B., Lee S.S., Lai S.T., Yuen K.Y., Choi K.W., Tso E.Y., Chau T.N., Tong W.L., Chiu M.C. and Yu W.C., PMH SARS Study Group. Coronavirus infection in an AIDS patient, AIDS. 2004, 18: 829-30.

Woo P.C.Y., Fung A.S.M., Lau S.K.P., Teng L.L., Wong B.H., Wong M.K., Hon E.H.C., Tang G.W.K. and Yuen K.Y., Actinomyces hongkongensis sp. nov. a novel Actinomyces species isolated from a patient with pelvic actinomycosis, Systematic and Applied Microbiology . 2003, 26(4): 518-22.

Woo P.C.Y., To A.P.C., Tse H., Lau S.K.P. and Yuen K.Y., Clinical and molecular epidemiology of erythromycin-resistant beta-hemolytic lancefield group G streptococci causing bacteremia, Journal of Clinical Microbiology . 2003, 41: 5188-91.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Chan K.H., Tam V.K., Peiris J.S.M. and Yuen K.Y., Detection of specific antibodies to severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein for serodiagnsosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2004, 42(5): 2306-9.

Woo P.C.Y., Tam D.M., Lau S.K.P., Fung A.M. and Yuen K.Y., Enterococcus cecorum empyema thoracis successfully treated with cefotaxime, Journal of Clinical Microbiology . 2004, 42: 919-22.

Woo P.C.Y., To A.P.C., Lau S.K.P. and Yuen K.Y., Facilitation of horizontal transfer of antimicrobial resistance by transformation of antibiotic-induced cell-wall-deficient bacteria, Medical Hypotheses . 2003, 61: 503-8.

Woo P.C.Y., Lau S.K.P., Fung A.M., Chiu S.K., Yung R.W. and Yuen K.Y., Gemella bacteraemia characterised by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology . 2003, 56: 690-3.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Woo P.C.Y., Kuhnert P., Burnens A.P., Teng L.L., Lau S.K.P., Que T.L., Yau H.H. and Yuen K.Y., Laribacter hongkongensis: a potential cause of infectious diarrhea, Diagnostic Microbiology and Infectious Disease . 2003, 47: 551-6.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Woo P.C.Y., Tse H., Chan K.M., Lau S.K.P., Fung A.M., Yip K.T., Tam D.M., Ng K.H., Que T.L. and Yuen K.Y., Streptococcus milleri" endocarditis caused by Streptococcus anginosus, Diagnostic Microbiology and Infectious Disease . 2004, 48: 81-8.

Woo P.C.Y., Zhen H., Cai J.J., Yu J., Lau S.K.P., Wang J., Teng L.L., Wong S.S.Y., Tse R.H., Chen R., Yang H., Liu B. and Yuen K.Y., The mitochondrial genome of the thermal dimorphic fungus Penicillium marneffei is more closely related to those of molds than yeasts, FEBS Letters . 2003, 555: 469-77.

Woo P.C.Y., Lau S.K.P., Wong S.S.Y. and Yuen K.Y., Two cases of continuous ambulatory peritoneal dialysis-associated peritonitis due to Plesiomonas shigelloides, Journal of Clinical Microbiology . 2004, 42: 933-5.

Yam W.C., Cheng V.C.C., Hui W.T., Wang L.N., Seto W.H. and Yuen K.Y., Direct detection of Mycobacterium tuberculosis in clinical specimens using single-tube biotinylated nested polymerase chain reaction-enzyme linked immunoassay (PCR-ELISA), Diagnostic Microbiology and Infectious Disease . 2004, 48: 271-5.

Yam W.C., Chan K.H., Poon L.L.M., Guan Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Evaluation of Reverse Transcription-PCR Assays for Rapid Diagnosis of Severe Acute Respiratory Syndrome Associated with a Novel Coronavirus, Journal of Clinical Microbiology. 2003, 41(10): 4521-4524.

Yuen K.Y., Antiviral Therapy for SARS., Combined Scientific Ammeting in Anaesthesiology 2003 (in Conjunction with the 4th Congress of the Asian Oceanic Society for Intravenous Anaesthesia), Hong Kong.. 2003.

Yuen K.Y., Antiviral therapy for SARS., Hong Kong Academy of Medicine - New Challenges in Healthcare.. 2003.

Yuen K.Y., Detection of human and animal SARS coronavirus., in International Conference on Influenza and the Resurgence of Severe Acute Respiratory Syndrome (SARS), Taipei, Taiwan.. 2003.

Yuen K.Y., Drug Research on Antimicrobial Agents., 7th Beijing Biotech & Pharm Industry Development Forum, Beijing, China.. 2003.

Yuen K.Y., Facing the unknows of SARS., 2003 International Science Symposium on SARS, Beijing, China.. 2003.

Yuen K.Y., Facing the unknows of SARS., 2003 Prevention and Control "SARS" (Guangzhou) Scientific Symposium, Guangzhou, China.. 2003.

Yuen K.Y., Immunization against SARS., Meeting on New Theories and Developments on Antimicrobial Vaccines, Shanghai, China.. 2003.

Yuen K.Y., Microbial Genomics, Symposium of the National Research Program for Genomic Medicine, Taipei.. 2004.

Yuen K.Y., New Findings on SARS., Second China-Japan Symposium on Cancer and Infectious Diseases.. 2004.

Yuen K.Y., Prophylaxis & Anti-viral agents., Hong Kong SARS Forum and Hospital Authority Convention 2004.. 2004.

Yuen K.Y., SARS - Coronavirus Pneumonia: Rationale for Antiviral Therapy., Chinese Medical Forum - SARS Prevention, Beijing, China.. 2003.

Yuen K.Y., SARS: market, toilet, hospital, and laboratory., Hong Kong Medical Journal. 2004, 10(3): 148-9.

Yuen K.Y., Severe Acute Respiratory Syndrome Associated Novel Coronavirus., The 6th Chinese Laboratory Medicine Conference, Hong Kong.. 2003.

Yuen K.Y., The discovery of an emerging pathogen., Hong Kong Academy of Medicine - New Challenges in Healthcare.. 2003.

Yuen K.Y., The importance of wild & domestic animals in emerging infections., Conference on Public Health and Inauguration of the World Association of Chinese Public Health Professionals.. 2004.

Yuen K.Y., Virology and Diagnostic Tests., 8th Hong Kong Medical Forum, hong Kong.. 2003.

Yuen K.Y., Virology and Pathogenesis of SARS., International Association of College and Academy Presidents (IACAP) Meeting, Hong Kong.. 2003.

Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Wang Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., The Complete Genome Sequence of Severe Acute Respiratory Syndrome Coronavirus Strain HKU-39849 (HK-39), Experimental Biology Medicine. 2003, 228: 866-873.

Zhong N.S., Zheng B., Li Y.M., Poon L.L.M., Xie Z.H., Chan K.H., Li P.H., Li P.H., Li P.H., Tan S.Y., Chang Q., Xie J.P., Liu X.Q., Xu J., Li D.X., Yuen K.Y., Peiris J.S.M. and Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003, The Lancet. 2003, 362(9393): 1353-8.

Researcher : Yung RWH

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai R.W., Hui W.T., Wong S.S.Y., Yau H.H. and Yuen K.Y., L Hongkongensis study group. Association of Laribacter hongkongensis in community-cquired gastroenteritis with travel and eating fish: a multicentre case-control study. , Lancet. 2004, 363(9425): 1941-7.

Woo P.C.Y., Lau S.K.P., Teng L.L., Que T.L., Yung R.W.H., Luk W.K., Lai W.M., Hui W.A.I.-.T.I.N.G., Wong S.S.Y., Yau H.O.N.-.H.U.N.G. and Yuen K.Y., Laribacter hongkongensis in fish is associated with gastroenteritis and traveller's diarrhoea, 14th European Congress of Clinical Microbiology and Infectious Diseases. Prague, The Czech Republic.. 2004.

Researcher : Zhang L

List of Research Outputs

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Researcher : Zheng B

Project Title:	CCR5 expression on antigen-presenting cens in HIV infection
Investigator(s):	Dr. Zheng B., Prof. Lee S.S.
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund
Start Date:	01/2001

Abstract:

To describe the pattern of CCR5 expression on APC in HIV infection; to correlate this pattern to HIV infected APC; to determine the functional and/or quantitative deficiency in CCR5 expressing APC.

Project Title:	The pattern of chemokine receptor polymorphism in healthy and HIV positive Chinese adults
Investigator(s):	Dr. Zheng B., Prof. Lee S.S.
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund
Start Date:	01/2001

Abstract:

To determine the prevalence of the major forms of CCR5 promoter and CCR2 polymorphism in healthy Chinese adults; to compare the pattern between healthy adults and HIV infected individuals in Hong Kong.

Project Title:	Functional studies of dendritic cells in chronic Chinese Hepatitis B patients
Investigator(s):	Dr. Zheng B., Prof. Qu D., Prof. Wen Y.M., Prof. Yuen K.Y., Dr. Zhang J.M., Dr. Zhu N.S.
Department:	Microbiology
Source(s) of Funding:	National Natural Science Foundation of China
Start Date:	12/2001

Abstract:

To ascertain if defective DC indeed contribute to CHBVI and if the defect may account for the immune tolerance; to investigate the mechanism of the DC functional deficit; to determine the extent to which it contributes to the infection; to investitate whether the defect may affectimmune response to other viruses or the general immune status of the individuals; to determine whether the defect may be overcome by ex vivo cytokine treatment to restore the function of DC and/or by improving antigen uptake by DC.

Project Title:	Development of oral DNA vaccine for immune therapy of chronic HBV infection
Investigator(s):	Dr. Zheng B., Prof. Yuen K.Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	01/2003
Completion Date:	12/2003

Abstract:

To determine if the oral DNA vaccine indeed induce an innate immune response and the kinetics and tissue distribution of this innate immune response in normal animals; to study the combined effects of the innate and specific adaptive immune responses to oral DNA vaccination in chronic HBV infection animal model, to evaluate the therapeutic efficacy of oral DNA vaccine delivering HBsAg and HBcAg which cloned from HBV Hong Kong strains in chronic HBV infection animal model; to construct oral DNA vaccines that can be used in human; to carry out clinical trial phase I in healthy volunteers.

Project Title:	Primary study for development of an inactivated coronavirus vaccine
Investigator(s):	Dr. Zheng B.
Department:	Microbiology
Source(s) of Funding:	SARS Research Fund
Start Date:	07/2003

Abstract:

To develop a coronavirus inactivated vaccine (CI-1) for the prevention of SARS-associated coronavirus infection.

Project Title:	A study of dendritic cell-based vaccine for immune therapy of chronic hepatitis B virus infection
Investigator(s):	Dr. Zheng B., Dr. Lu L.W., Prof. Ng M.H.
Department:	Microbiology
Source(s) of Funding:	RGC Projects (Block Grant Funded)
Start Date:	07/2003

Abstract:

To investigate the hypothesis that DC-based vaccine (cytokine-activated monocyte-derived DCs used as vectors to deliver HBsAg or HBcAg) may be crucial in immunotherapy of chronic HBV infection.

Project Title:	A study of antiviral peptides targeting spike and matrix proteins of SARS coronavirus
Investigator(s):	Dr. Zheng B., Dr. Guan Y.
Department:	Microbiology
Source(s) of Funding:	Hong Kong Research Grants Council Competitive Earmarked Research Grants
Start Date:	09/2003
Completion Date:	08/2004

Abstract:

To test the hypothesis that some of amino acid residues in spike (S) and matrix (M) proteins that are different between human SCoV and animal SCoV-like viruses might determine whether the virus infects human or animals and that peptides designed based on these substitutions might interfere with the formation of fusion-active structures and therefore block the entry of the virus into host cells.

Project Title:	Studies of SARS virus vaccines
Investigator(s):	Dr. Zheng B., Dr. Guan Y.
Department:	Microbiology
Source(s) of Funding:	Block Grant Earmarked for Research
Start Date:	02/2004

Abstract:

To develop SCoV vaccines for prevention of SARS; to develop an inactivated vaccine and a cold attenuated live vaccine.

List of Research Outputs

Cheng V.C.C., Hung I.F.N., Tang B.S., Chu C.M., Wong M.M., Chan K.H., Wu A.K., Wu A.K., Tse D.M., Chan K.S., Zheng B., Peiris J.S.M., Sung J.J. and Yuen K.Y., Viral replication in the nasopharynx is associated with diarrhea in patients with severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 38(4): 467-75.

Guan Y., Zheng B., He Y.Q., Liu X.L., Zhuang Z.X., Cheung C.L., Luo S., Li P.H., Zhang L., Guan Y., Butt K.M., Wong K.L., Chan K.W., Lim W.W.L., Shortridge K.F., Yuen K.Y., Peiris J.S.M. and Poon L.L.M., Isolation and characterization of viruses related to the SARS Coronavirus from Animals in Southern China., Science. 2003, 302(5643): 276-278.

Guan Y., Peiris J.S.M., Zheng B., Poon L.L.M., Chan K.H., Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Leung C.S.W., Yuen K.Y. and Leung F.C.C., Molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome, The Lancet. 2004, 363: 99-104.

Poon L.L.M., Chan K.H., Nicholls J.M., Zheng B., Yuen K.Y., Guan Y. and Peiris J.S.M., Characterization of a novel coronavirus responsible for severe acute respiratory syndrome, Options for the Control of Influenza V. 2004, International Congress Series 1263: 805-808.

Poon L.L.M., Chan K.H., Wong O.K., Cheung K.W., Ng I.H.Y., Zheng B., Seto W.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays., Clinical Chemistry. 2004, 50(1): 67-72.

Sun H., Huang J. and Zheng B., Bismuth as an Enzyme Inhibitor: From Antibacterium to Antivirus, Abstract of The 8th International Symposium on Applied Bioinorganic Chemistry, April 2-5, 2004 . 2004, IL15.

Wong A.T., Tsang O.T., Wong M.Y., Lim W.L., Zheng B., Lee S.S., Lai S.T., Yuen K.Y., Choi K.W., Tso E.Y., Chau T.N., Tong W.L., Chiu M.C. and Yu W.C., PMH SARS Study Group. Coronavirus infection in an AIDS patient, AIDS. 2004, 18: 829-30.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chan K.H., Wong B.H., Che X.Y., Tam V.K., Tam S.C., Cheng V.C.C., Hung I.F., Wong S.S.Y., Zheng B., Guan Y. and Yuen K.Y., Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia, Lancet. 2004, 363: 841-5.

Zeng F., Chan W.M., Chan M.N., Chen J., Chow Y.C.K., Hon C.C., Hui K.H., Li J., Li Y.Y., Wang Y., Wang Y., Guan Y., Zheng B., Poon L.L.M., Chan K.H., Yuen K.Y., Peiris J.S.M. and Leung F.C.C., The Complete Genome Sequence of Severe Acute Respiratory Syndrome Coronavirus Strain HKU-39849 (HK-39), Experimental Biology Medicine. 2003, 228: 866-873.

Zheng B., Guan Y., Tang Q., Cheng D., Xie F.Y., He M.L., Chan K.W., Wong K.L., Lader E., Woodle M.C., Lu P.Y., Li B. and Zhong N., Prophylactic and therapeutic effects of small interfering RNA targeting SARS-coronavirus, Antiviral Therapy. 2004, 9: 365-374.

Zheng B., Guan Y., Wong K.H., Zhou J., Wong K.L., Young B.W.Y., Lu L. and Lee S.S., SARS-related virus predating SARS outbreak, Hong Kong, Emerging Infectious Diseases. 2004, 10(2): 176-178.

Zheng B., Zhou J., Qu D., Siu K.L., Lam T.W., Lo H.Y., Lee S.S. and Wen Y.M., Selective Functional Deficit in Dendritic CEll - T Cell interaction Is a Crucial Mechanism in Chronic Hepatitis B Virus Infection, Journal of Viral Hepatitis. 2004, 11: 217-224.

Zhong N.S., Zheng B., Li Y.M., Poon L.L.M., Xie Z.H., Chan K.H., Li P.H., Li P.H., Li P.H., Tan S.Y., Chang Q., Xie J.P., Liu X.Q., Xu J., Li D.X., Yuen K.Y., Peiris J.S.M. and Guan Y., Epidemiology and cause of severe acute respiratory syndrome (SARS) in Guangdong, People's Republic of China, in February, 2003, The Lancet. 2003, 362(9393): 1353-8.

Researcher : Zhou J

List of Research Outputs

Zheng B., Guan Y., Wong K.H., Zhou J., Wong K.L., Young B.W.Y., Lu L. and Lee S.S., SARS-related virus predating SARS outbreak, Hong Kong, Emerging Infectious Diseases. 2004, 10(2): 176-178.

Zheng B., Zhou J., Qu D., Siu K.L., Lam T.W., Lo H.Y., Lee S.S. and Wen Y.M., Selective Functional Deficit in Dendritic CEll - T Cell interaction Is a Crucial Mechanism in Chronic Hepatitis B Virus Infection, Journal of Viral Hepatitis. 2004, 11: 217-224.

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