Dept of Pharmacology

DEPT OF PHARMACOLOGY

Researcher : Chan MLY

List of Research Outputs

Chan M.L.Y., Keung W.W.Y., Leung S.W.S., Che C.M. and Man R.Y.K., Role of the nitric oxide signaling cascade in the biological actions of traditional chinese medicine, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Researcher : Cho CH

Project Title:	Studying the ulcerogenic action of dexamethasone and its relationship with PGE2 on ulcer healing in the stomach
Investigator(s):	Cho CH
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2005

Abstract:

To apply the non-ulcerogenic doses of dexamethasone, a potent corticosteroid, to explore its action on ulcer healing and the mechanistic pathway related to prostaglandin E[2] synthesis in a rat ulcer model in the stomach.

Project Title:	Delineation of the contributory role of EGF/polyamine/K+ channel in gastric mucosal repair
Investigator(s):	Cho CH, Li GR, Wong TM, Wang J.Y.
Department:	Pharmacology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003
Completion Date:	11/2005

Abstract:

To clarify the integrating roles of EGF and polyamines together with the KV channels and the ca²⁺cy⁺ in gastric mucosal repair; to elucidate the mechanism of how cigarette smoke and its ingredien + affect ulcer healing.

Project Title:	Delineation of the contributory role of EGF/polyamine/K+ channel in gastric mucosal repair
Investigator(s):	Cho CH, Li GR, Wong TM, Wang J.Y.
Department:	Pharmacology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003
Completion Date:	11/2005

Abstract:

N/A

Project Title:	Nicotine and its active metabolite in the tumorigenesis of colon cancer
Investigator(s):	Cho CH, Chan K.K.C.
Department:	Pharmacology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2004
Completion Date:	10/2006

Abstract:

To investigate the differential roles of nicotinic receptor and B-adrenoceptor in the tumorigenesis of colon cancer cells; to investigate pathogenic signal pathway of nicotine and its metabol NNK in the development of colon cancer; to investigate the therapeutic strategy to target the oncogenes involved in this carcinogenic pathway to treat and prevent cancer formation especially in cigarette smokers.

Project Title:	Nicotine and its active metabolite in the tumorigenesis of colon cancer
Investigator(s):	Cho CH
Department:	Pharmacology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

N/A

Project Title:	Cathelicidins and gastric ulcer healing
Investigator(s):	Cho CH
Department:	Pharmacology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005
Completion Date:	01/2006

Abstract:

(1) To determine the time course of rCRAMP expression at the ulcer site of acetic acid-induced gastric ulcers; (2) To determine if increased rCRAMP expression by gene therapy accelerates gastric ulcer healing; (3) To determine if decreased rCRAMP expression by antisense Oligonucleotide delays ulcer healing; (4) To examine the possible mechanisms involved in the gastric ulcer healing by rCRAMP Finally, we will test the antimicrobial activity of rCRAMP against E. coli, Streptococcus and Enterococcus.

Project Title:	Cyclooxygenase-2 and its upstream signal transduction mediators in the promotion of gastric cancer development by cigarette smoke
Investigator(s):	Cho CH
Department:	Pharmacology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2005
Completion Date:	01/2007

Abstract:

N/A

Project Title:	Cyclooxygenase-2 and its upstream signal transduction mediators in the promotion of gastric cancer development by cigarette smoke
Investigator(s):	Cho CH, Chu KM
Department:	Pharmacology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2005
Completion Date:	01/2007

Abstract:

To provide direct evidence that CS and nicotine does indeed promote gastric cancer growth; to define the mechanistic cascade from CS to gastric cancer growth; to substantiate the importance of COX-2 and its upstream signal transduction mediators in gastric cancer development in cigarette smokers.

Project Title:	Adrenaline and its receptor activation on colon cancer growth
Investigator(s):	Cho CH
Department:	Pharmacology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2006

Abstract:

B-Adrenoceptor, a G-protein coupled receoptr, has recently been implicated in the carcinogenesis of different kinds of cancers. It has been reported that actiavtion of B-adrenoceptors could stimulate pulmonary, pancreatic and colon carcinoma cell growth ( Carcinogenesis 1898, 10:1753; Carcinogenesis 2001, 22: 473; Cancer Res. 2005, 65: 5272). Polymorphisms in B-adrenoceptor genes are also associated with increased risk of breast, colon and endometrial cancers (Cancer Res. 2001, 3:264; Int. J. Clin. Oncol 2001, 6:117; Obstet. Gynecol 2003, 102: 506; J.Exp. Clin. Cacner Res. 2004, 23: 669). In addition, B-blockers could inhibit the migration of colon cancer cells induced by noradrenaline (Cancer Res. 2001, 61: 2866). Recent clincial studies also reveal that using B-blockers is negatively associated cancer risk (Rev. Epidemiol. Sante Publiqu 2004, 52: 53; Lancet 1997, 349: 525; Am. J. Hypertens. 1996, 9: 695). All thes findings converge to suggest that adrenoceptors especially B-adrenoceptors indeed play a crucial role in the development of cancer. On the other hand, biobehavioral stresses may influence growth and progression of cancer (Clin. Can. Res. 2003, 9:4514). It has also been demonstrated that altered hormonal and neuronal secretion during stress have a strong impact on the biological activities of breast cancer (Breast Can. Res. Treat. 2003, 80: 63). Among the various stress hormones, adreanline and noradrenaline play an important role in mediating the effect of stress on target cells via adrenergic receptors (Caner 2003, 98: 1547). Indeed our recent study demostrates that cigarette smoke which has been closely associated with cancer development also stimulates colon cancer growth through B-adrenoceptors activation (Cancer Res. 2005, 65:5272). The relationship between stress mediator adrenaline and B-adrenoceptor on colon cancer growth has not been defined. In this study we shall delineate this action and the signal transduction pathway involved will also be investigated. In addition we shall examine the pharmacological action of nicotine, the major active component in cigarette smoke also shown to stimulate adrenaline release in the body, on colon cancer growth.

List of Research Outputs

Chan O.O., Chu K.M., Leung S.Y., Huang C., Sun Y.W., Ko S., Yuen R.M.F., Xia H.H.X., Cho C.H., Hui W.M., Lam S.K., Rashid A. and Wong B.C.Y., Helicobacter pylori infection, interleukin 1 beta polymorphism and predisposition to gastric cancer through the CpG island methylation pathway, Presented at 11th Medical Research Conference, Department of Medicine, The University of Hong Kong, Hong Kong, 14 January 2006.

Chan O.O., Chu K.M., Leung S.Y., Huang C., Sun Y., Ko S., Yuen R.M.F., Xia H.H.X., Cho C.H., Hui W.M., Lam S.K., Rashid A. and Wong B.C.Y., Helicobacter pylori Infection, Interleukin 1 Beta Polymorphism and Predisposition to Gastric Cancer Through the Cpg Island Methylation, Gastroenterology. 2006, 130 (4 Suppl 2): A-717.

Cho C.H., Cathelicidin: a molecule for antimicrobial or for ulcer healing in the stomach , Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July;Suppl 1: 6.

Cho C.H. and Shin V.Y., Nicotine and gastric cancer, International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 16.

Guo J., Chau F. .L., Cho C.H. and Koo M.W.L., Worsening effect of partial sleep deprivation on indomethacin-induced gastric mucosal damage, Pharmacology, Biochemistry and Behavior . Elsevier Inc, 2005, 82: 515-521.

Guo J.S., Cho C.H., Wang J.Y. and Koo M.W.L., Differential effects of selective and non-selective inhibition of nitric oxide synthase on the expression and activity of cyclooxygenase-2 during gastric ulcer healing, European Journal of Pharmacology. 2006, 536: 301-308.

Hui K.C., Wu K.K., Shin V.Y., Cho C.H. and So H.L., Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice , International Journal of Medical Sciences. 2006, 2006;3: 1-6.

Ko J.K.S., Tin M.M., Cho C.H. and Chan K.K., Astragalus saponins induce inhibition of cell proliferation in association with COX-independent overexpression of NAG-1 in colon cancer cells, Supplement to Gastroenterology - Digestive Disease Week and the 107th Annual Meeting of the America Gastroenterological association Institute, May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-583.

Kopaniszen M., Tai K.K., Lam K.Y., So H.L., Cho C.H. and Koo M.W.L., The effects of green tea catechin on DNBS-induced ulcerative colitis in mice, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 73(suppl 2): 37.

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Lam K.Y., Wu K.K., Wong P.S.H., Tai K.K., Shin V.Y., So H.L. and Cho C.H., Lactobacillus GG enhances gastric ulcer healing via attenuation of cellular apoptosis and promotion of angiogenesis and cell proliferation, International Symposium on Translational Research, Trondheim, Norway December 9-10, 2005. 12.

Li G., Xia H.H.X., Chen M., Gu Q., Wang J., Peng J., Chan O.O., Cho C.H., So H.L., Lam S.K., Hu P.J., Liang Y.J., Lin H., Berg D.E., Feng Z.H., Langenback R. and Wong B.C.Y., Effects of Cyclooxygenase-1 and -2 Gene Disruption on Helicobacter pylori-Induced Inflammation, Journal of Infectious Diseases. 2006, 193(7): 1037-1046.

Mak J.C.W., Hui W.S., Ho S.P., So W.H.L., Chan M.M.W., Cho C.H. and Lam W.K., Inducation of CINC-1 in the lung tissue from rats exposed to cigarette smoke, 11th Medical Research Conference, Department of Medicine, The University of Hong Kong. 2006, Abstract Book 088: pp. 55.

Mak J.C.W., Hui W.S., Ho S.P., So W.H., Chan M.M.W., Cho C.H. and Lam W.K., Induction of CINC-1 in the lung tissue from rats exposed to cigarette smoke, American Journal of Respiratory and Critical Care Medicine. 2006, 173(3): A634.

Shin V.Y., Wu K.K., Chu K.M., Wong P.S.H., Lam K.Y., Tai K.K., Koo M.W.L. and Cho C.H., Nicotine induces cyclooxygenase-2 and vascular endothelial growth factor receptor-2 in association with tumor-associated invasion and angiogenesis in gastric cancer, Mol Cancer Res. 2005, 3(11): 607-15.

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

Tai K.K., Wu K.K. and Cho C.H., The role of cathelicidins in inflammation and wound healing, Ulcer Research. 2006, 33:1-2006: 54-60.

Tang L.P., Cho C.H., Hui W.M., Huang F.Y., Lai C.L., Xia H.H.X., Lam S.K., Rashid A., Wong B.C.Y. and Chan O.O., A negative correlation between IL-6 and DNA methylation in patients with gastric cancer, Supplement to Gastroenterology - Digestive Disease Week and the 107th Annual Meeting of the American Gastroenterological Association Institute, May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-717.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Wong P.S.H., Wu K.K., Tai K.K., Lam K.Y., Shin V.Y., So H.L. and Cho C.H., Beta-adrenoceptors mediate colon cancer cell proliferation induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 22.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K. and Cho C.H., Involvement of K_v1.1 and Na_v1.5 in proliferation of gastric epithelial cells, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan . 2006, 2006;73 (suppl 2): 6.

Wu K.K., Wong H.P.S., Ye Y. and Cho C.H., Nicotine and Cancer, Alcohol, Tobacco and Cancer. Karger, 2006, 253-267.

Wu K.K., Yang Y., Tai K.K., Wong H.P.S., Lam K.Y., Shin V.Y. and Cho C.H., The antimicrobial peptide cathelicidin promotes gastric ulcer healing in rats, Supplement to Gastroenterology - Digestive Disease Week and the 107th annual Meeting of the American Gastroenterological Association Institute May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-543.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Ye Y., Wu K.K., Shin V.Y. and Cho C.H., A mechanistic study of colon cancer growth promoted by cigarette smoke extract, European Journal of Pharmacology. 2005, 519 (2005): 52-57.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Choy PC

List of Research Outputs

Ng W.W.H., Man R.Y.K., Choy P.C. and Leung S.W.S., Genistein induces nitric oxide release from vascular endothelial cells, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Researcher : Fu Y

List of Research Outputs

Fu Y. and Koo M.W.L., EGCG protects HT-22 cells against glutamate-induced oxidative stress, Neurotoxicity Research . 2006, 10(1): 23-30.

Researcher : Guo J

List of Research Outputs

Guo J., Chau F. .L., Cho C.H. and Koo M.W.L., Worsening effect of partial sleep deprivation on indomethacin-induced gastric mucosal damage, Pharmacology, Biochemistry and Behavior . Elsevier Inc, 2005, 82: 515-521.

Researcher : Hui KC

List of Research Outputs

Hui K.C., Wu K.K., Shin V.Y., Cho C.H. and So H.L., Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice , International Journal of Medical Sciences. 2006, 2006;3: 1-6.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Researcher : Keung WWY

List of Research Outputs

Chan M.L.Y., Keung W.W.Y., Leung S.W.S., Che C.M. and Man R.Y.K., Role of the nitric oxide signaling cascade in the biological actions of traditional chinese medicine, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Man R.Y.K., Leung S.W.S., Teoh H., Quan A., Keung W.W.Y. and Lee M.Y.K., Phytoestrogens and cardiovascular disorders, Pathophysiology of Cardiovascular Disease. Kluwer Academic Publishers, 2005, IV: 513-524.

Researcher : Keung WYW

List of Research Outputs

Keung W.Y.W., Vanhoutte P.M.G.R. and Man R.Y.K., Nongenomic responses to 17b-estradiol in male rat mesenteric arteries abolish intrinsic gender differences in vascular responses, British Journal of Pharmacology. Nature, 2005, 146: 1148-1155.

Researcher : Ko JKS

List of Research Outputs

Ko J.K.S., Tin M.M., Cho C.H. and Chan K.K., Astragalus saponins induce inhibition of cell proliferation in association with COX-independent overexpression of NAG-1 in colon cancer cells, Supplement to Gastroenterology - Digestive Disease Week and the 107th Annual Meeting of the America Gastroenterological association Institute, May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-583.

Researcher : Koo MWL

Project Title:	Effects of antioxidants and the roles of heat shock protein 32 on cognitive functions in aging
Investigator(s):	Koo MWL
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002

Abstract:

To carify the roles of HOs in aging and its involvement in cognitive functions; to elucidate the usefulness of antioxidants in delaying cognitive function loss during aging.

Project Title:	Alleviating effect of Bu-zhong-yi-qi-tang on chemotherapy-induced bone marrow suppression in mice
Investigator(s):	Koo MWL
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To investigate the mechanisms of action from herbs particularly on bone marrow recovery and CSFs production.

Project Title:	Effect of tea on lipoprotein lipase and lipid metabolism
Investigator(s):	Koo MWL
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	03/2006

Abstract:

Key issues and problems More and more people in developed countries are becoming obese. In the United States, about a third of adults are obese with a body mass index above 27 kg/m2. The prevalence is increasing in all major races and sex groups including younger adults age 25 to 44. Obesity is also an important risk factor for the development of cardiovascular diseases. A positive imbalance of energy intake and expenditure resulted in obesity (1, 2). Dietary intake of free fatty acid (FFA) are esterified to form triglyceride (TG) and assembled into large lipoprotein particles (chylomicrons), which are excreted from intestinal cells into lymph and then to the general circulation. TC rich, very low density lipoproteins (VLDL), are assembled in a similar manner in the liver and secreted into the blood. Both of these TG rich particles (chylomicrons and VLDL) are substrates for the lipolytic action of lipoprotein lipase (LPL) as they pass through the capillary space and be converted into low density lipoproteins (LDL) (3, 4). LPL is synthesized by fat cells and its activity is regulated by catecholamines and insulin. We have shown that chronic tea consumption reduced cAMP level in fat cells (5), thus tea may modulate LPL activity and lower the risk of atherosclerosis. LDL when oxidized and taken up by the macrophages will become foam cells and deposited in the arterial walls, therefore excessive consumption of high fat diet can lead to atherosclerosis and cardiovascular diseases. In our previous studies, we have shown that tea drinking can decrease plasma cholesterol level (6) prevent low density lipoprotein (LDL) oxidation and elevate the high density lipoprotein to total cholesterol ratio (7). Animals given tea treatment have a lower risk of atherosclerosis induced by feeding with hypercholesterol diet (7). Recently, one of our population studies in Hong Kong has found a strong inverse correlation of tea consumption with obesity (1). Unpublished data obtained from our clinical investigation on obese patients has suggested an inhibitory effect of tea on lipoprotein lipase, which is important for the metabolism of very low density lipoproteins and contributes to cardiovascular disorders (8). There is also report of tea in suppressing the activities of other lipase systems in the gastrointestinal tract (9). Since cardiovascular disorders induced more death than another other diseases in Hong Kong and tea seems to be beneficial in preventing these disorders, it is thus important to establish the health-promoting role of tea in a more scientific manner. Purpose of the project The objectives of this project are to investigate if tea consumption can inhibit lipoprotein lipase in rats fed with hypercholesterol diet and reduce the risk of atherosclerosis since lipoprotein lipase is required also for the uptake of oxidized LDL into the blood vessel wall. References: 1. Fong, KK. (2004). Correlation of obesity with tea consumption in a Hong Kong general practice population: a cross-sectional survey. M. Med. Sc. dissertation, The University of Hong Kong 2. Despres JP., Moorjani S., Lupien PJ., et al., (1990). Regional distribution of body fat, plasma lipoproteins, and cardiovascular disease. Arteriosclerosis, 10:493-6 3. Bjorntorp, P., Bradoff BN. (1992). In: Obesity, Philadelphia Penn: Lippincott JB Company, pp3-12 4. Dan M. (2001). Effect of green tea on diet-induced obesity in rats. M.Med.Sc. dissertation, The University of Hong Kong] 5. Chiu WY. (2002). Effect of chronic green tea consumption on lipolysis in rats. M.Med.Sc. Dissertation, The University of Hong Kong 6. Yang, TTC., Koo, MWL. (2000). Chinese green tea lowers cholesterol level through an increase in fecal lipid excretion. Life Sci. 66:411-4231 7. Yang, TTC., Koo, MWL. (2000). Inhibitory effect of Chinese green tea on endothelial cell-induced LDL oxidation. Atherosclerosis 148:67-732 8. Chan, CCW., Koo, MWL., Ng, EHY., Tang, OS., Yeung WSB., Ho, PC. Effects of Chinese green tea on weight, hormonal and biochemical profiles in obese patients with polycystic ovary syndrome – a randomized placebo controlled trial 9. Chantre, P., Laison, D. (2002). Recent findings of green tea extract AR25 (Exolise) and its activity for the treatment of obesity. Phytomedicine, 9:3-8

List of Research Outputs

Chan C., Chan W.K., Law H.K.W., Wong W.T., Koo M.W.L., Lau A.S.Y., Lau Y.L. and Chan G.C.F., Ganoderma lucidum mycelium extract can enhance maturation of human monocyte-derived dendritic cells, 37^th Annual Conference of The International Society of Paediatric Oncology SIOP 2005, Vancouver, Canada, 21-24 September 2005 (Abstract in Pediatric Blood & Cancer). 2005, 45(4): 577.

Chan W.K., Lam T.W., Law H.K.W., Wong W.T., Koo M.W.L., Lau A.S.Y., Lau Y.L. and Chan G.C.F., Ganoderma lucidum mycelium and spore extracts as natural adjuvants for immunotherapy, The Journal of Alternative and Complementary Medicine. 2005, 11(6): 1047-1057.

Fu Y. and Koo M.W.L., EGCG protects HT-22 cells against glutamate-induced oxidative stress, Neurotoxicity Research . 2006, 10(1): 23-30.

Guo J., Chau F. .L., Cho C.H. and Koo M.W.L., Worsening effect of partial sleep deprivation on indomethacin-induced gastric mucosal damage, Pharmacology, Biochemistry and Behavior . Elsevier Inc, 2005, 82: 515-521.

Guo J.S., Cho C.H., Wang J.Y. and Koo M.W.L., Differential effects of selective and non-selective inhibition of nitric oxide synthase on the expression and activity of cyclooxygenase-2 during gastric ulcer healing, European Journal of Pharmacology. 2006, 536: 301-308.

Kopaniszen M., Tai K.K., Lam K.Y., So H.L., Cho C.H. and Koo M.W.L., The effects of green tea catechin on DNBS-induced ulcerative colitis in mice, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 73(suppl 2): 37.

Shin V.Y., Wu K.K., Chu K.M., Wong P.S.H., Lam K.Y., Tai K.K., Koo M.W.L. and Cho C.H., Nicotine induces cyclooxygenase-2 and vascular endothelial growth factor receptor-2 in association with tumor-associated invasion and angiogenesis in gastric cancer, Mol Cancer Res. 2005, 3(11): 607-15.

Researcher : Kopaniszen M

List of Research Outputs

Kopaniszen M., Tai K.K., Lam K.Y., So H.L., Cho C.H. and Koo M.W.L., The effects of green tea catechin on DNBS-induced ulcerative colitis in mice, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 73(suppl 2): 37.

Researcher : Ku DD

List of Research Outputs

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelial dysfunction in the femoral artery from one-year old rats, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 137.

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Oxygen-derived free radicals mediate endothelium-derived contractions in the femoral artery from streptozotocin-treated rats, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7,2006 Beijing, China. 2006, 2006 July; Suppl 1: 170.

Shi Y., Feletou M., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., The calcium honophore A23187 induces endothelium-dependent contractions in femoral arteries from rats with streptozotocin-induced diabetes, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 51.

Tang H.C., Ku D.D., Tipoe G.L., Feletou M., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelium-dependent contractions occur in the aorta of wild-type and COX2^-/- knockout but not COX1^-/- knockout mice, Journal of Cardiovascular Pharmacology. 2005, 46(6): 761-765.

Researcher : Lam KY

List of Research Outputs

Kopaniszen M., Tai K.K., Lam K.Y., So H.L., Cho C.H. and Koo M.W.L., The effects of green tea catechin on DNBS-induced ulcerative colitis in mice, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 73(suppl 2): 37.

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Lam K.Y., Wu K.K., Wong P.S.H., Tai K.K., Shin V.Y., So H.L. and Cho C.H., Lactobacillus GG enhances gastric ulcer healing via attenuation of cellular apoptosis and promotion of angiogenesis and cell proliferation, International Symposium on Translational Research, Trondheim, Norway December 9-10, 2005. 12.

Shin V.Y., Wu K.K., Chu K.M., Wong P.S.H., Lam K.Y., Tai K.K., Koo M.W.L. and Cho C.H., Nicotine induces cyclooxygenase-2 and vascular endothelial growth factor receptor-2 in association with tumor-associated invasion and angiogenesis in gastric cancer, Mol Cancer Res. 2005, 3(11): 607-15.

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Wong P.S.H., Wu K.K., Tai K.K., Lam K.Y., Shin V.Y., So H.L. and Cho C.H., Beta-adrenoceptors mediate colon cancer cell proliferation induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 22.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K., Yang Y., Tai K.K., Wong H.P.S., Lam K.Y., Shin V.Y. and Cho C.H., The antimicrobial peptide cathelicidin promotes gastric ulcer healing in rats, Supplement to Gastroenterology - Digestive Disease Week and the 107th annual Meeting of the American Gastroenterological Association Institute May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-543.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Lam KY

List of Research Outputs

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Researcher : Lau CT

List of Research Outputs

Lau C.T. and Man R.Y.K., Isoflavone metabolite equol enhanced endothelium-independent relaxation, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. OP14.

Researcher : Law TY

List of Research Outputs

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Lee MYK

List of Research Outputs

Lee M.Y.K., Tse H.F., Man R.Y.K. and Vanhoutte P.M.G.R., Genomic changes in cultured porcine regenerated coronary endothelial cells, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 21.

Lee M.Y.K., Holmskov U., Man R.Y.K. and Vanhoutte P.M.G.R., Reduced up regulation of SP-D in response to TNF-a in senescent endothelial cells, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 171.

Leung S.W.S., Lee M.Y.K., Xu Y. and Man R.Y.K., Phytoestrogens and vascular responsiveness, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006. 2006.

Man R.Y.K., Leung S.W.S., Teoh H., Quan A., Keung W.W.Y. and Lee M.Y.K., Phytoestrogens and cardiovascular disorders, Pathophysiology of Cardiovascular Disease. Kluwer Academic Publishers, 2005, IV: 513-524.

Researcher : Lee YKM

List of Research Outputs

Lee Y.K.M., Tse H.F., Man R.Y.K. and Vanhoutte P.M.G.R., Genomic changes in culltured porcine regenrated coronary endothelial cells, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP15.

Researcher : Leung GPH

Project Title:	Adenosine: signal transduction pathways and role of nucleoside transporter re-uptake in coronary artery
Investigator(s):	Leung GPH, Man RYK
Department:	Pharmacology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	08/2004
Completion Date:	02/2006

Abstract:

To identify the signal transduction pathways of the adenosine-induced hyperpolarization in the coronary arterial smooth muscle cells and endothelial cells. We hypothesize that the adensoine-induced hyperpolarization in vascular smooth muscle cells and endothelial cells is mediated via cAMP-independent pathways and involves more than one K⁺ channels; to identify the mechanism of regulation of extracellular adenosine level in the coronary arterial smooth muscle cells and endothelial cells.

Project Title:	Cloning and characterization of novel equilibrative nucleoside transporter type 4 (ENT4)
Investigator(s):	Leung GPH
Department:	Pharmacology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	03/2005

Abstract:

Background: The physiological important nucleoside, adenosine, acts through adenosine receptors to exert diverse effects on cellular functions such as inhibition of platelet aggregation, slowing of heart rate and vasodilation. Nucleoside transporters are important in adenosine functions by fine-tuning its concentration in the vicinity of adenosine receptors. There are two classes of nucleoside transporters: Na+-dependent concentrative nucleoside transporters (CNTs) and Na+-independent equilibrative nucleoside transporters (ENTs). So far three equilibrative nucleoside transporters (ENTs) have been cloned and characterized. Both ENT1 and ENT2 are broadly selective, transporting purine and pyrimidine nucleosides. Nevertheless, ENT1 is nitrobenzylmercaptopurine ribonucleoside (NBMPR)-sensitive and has an IC50 of 1nM while ENT2 is relatively NBMPR-insensitive and has an IC50 > 1µM. Unlike ENT1 and ENT2, which are plasma membrane proteins, ENT3 is an intracellular protein. It may be responsible for salvaging nucleosides between the cytoplasm and the lumen of Golgi apparatus. Adenosine transport in endothelial cells is Na+-independent. Interestingly, we found that in physiological levels of adenosine (0.1 to 1µM), high concentrations of NBMPR (> 200µM, a concentration that can completely inhibit both ENT1 and ENT2) only reduce 20% of the adenosine transport. It indicates that another nucleoside transporter, which is characteristically distinct from the ENT1 and ENT2, is present in the endothelial cells. Recently, we have cloned a protein from endothelial cells which has 18% amino acid identity with ENT1. We term it ENT4 and hypothesize that it may be a novel isoform of nucleoside transporter. Objectives: In this grant application, we propose to characterize ENT4. In aim I, we will stably transfect ENT4 into nucleoside transporter-deficient cells. In aim II, we will functionally characterize ENT4. We will determine its affinities to different nucleosides and its sensitivities to different inhibitors such as NBMPR. In aim III, we will study the tissue distribution of ENT4.

Project Title:	Physiological and pharmacological studies of equilibrative nucleoside transporter-2
Investigator(s):	Leung GPH, Man RYK
Department:	Pharmacology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	03/2006

Abstract:

Adenosine is an endogenous purine nucleoside and modulates a variety of physiological functions by interacting with the adenosine receptors on cell surface. Under adverse conditions such as ischemia, hypoxia, stress and inflammation, extracellular levels of adenosine are increased. The increased extracellular adenosine protects tissues from excessive damage. It has been demonstrated that adenosine attentuates the ischemic heart injury, reduces inflammation and is vasodilatory. However, the therapeutic application of adenosine is limited because extracellular adenosine usually disappears quickly due to its rapid uptake into adjacent cells and subsequent metabolism. Adenosine is taken up from the extracellular space into adjacent cells through the nucleoside transporters on plasma membrane. Equilibrative nucleoside transporter (ENT)-1 and ENT2 are the major nucleoside transporters found in heart, endothelial cells and vascular smooth muscle cells. Physiologically, ENT1 is regulated by protein kinase A, protein kinase C, tyrosine kinase, nitrogen-activating protein kinase and casein kinase II. Pharmacologically, ENT1 is potently inhibited by nitrobenzylmercaptopurine riboside (NBMPR), dipyridamole and dilazep. In contrast, little is known about the physiological regulation of ENT2. Specific inhibitor for ENT2 is also not available. Therefore, in this grant application, we propose to 1) study the regulation of ENT2 2) screen for the potential lead compounds for the development of specific ENT2 inhibitor.

List of Research Outputs

Leung G.P.H., Adenosine transporters in cardiovascular system, Frontiers in Biomedical Research-HKU 2005, Hong Kong December 2. 2005, 63.

Leung G.P.H., Man R.Y.K. and Tse C.M., Effect of thiazolidinediones on equilibrative nucleoside transporter-1 in human aortic smooth muscle cells. , Biochemical Pharmacology. 2005, 70: 355-362.

Leung G.P.H., Transporters and vascular disease, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 24.

Li R.W.S., Man R.Y.K., Tse C.M. and Leung G.P.H., Effects of calcium antagonists on human equilibrative nucleoside transporters, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 361.

Seto S.W., Lau Y.M., Chiang H.K., Lam H.H., Chiu W.K., Ng H.Y., Kwan Y.W., Ngai S.M. and Leung G.P.H., Comparison of vascular relaxation and lipolytic effect of thiazolidinediones in diabetic (+db/+db) and non-diabetic (+db/+m) mice, Journal of the Hong Kong College of Cardiology. 2005, 13 No.2: 118, OP85.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Prostanoid synthases and receptors in aorta of spontaneously hypertensive rat, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 138.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Real-time quantitative PCR study of genes involved in the generation of endothelium-depedent contractions in the aorta of the spontaneously hypertensive rat, FASEB Journal. 2006, 20(4): A290-A291 Part 1.

Xu Y., Leung S.W.S., Leung G.P.H. and Man R.Y.K., Kaempferol, a compound from chinese medicine, potentiated relaxation in porcine arteries via cAMP pathway and activation of potassium channel, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 338.

Xu Y., Leung S.W.S., Leung G.P.H., Vanhoutte P.M.G.R. and Man R.Y.K., Structure activity relationship of phytoestrogens and their modulation of relaxing responses in porcine coronary arteries, 2006 AAPS National Biotechnology Conference, Boston, MA, June 18-21, 2006 . 2006, W3028: 112.

Xu Y.C., Leung G.P.H., Leung S.W.S., Vanhoutte P.M.G.R. and Man R.Y.K., Stimulation of large-conductance calcium-activated potassium channels in cultured endothelial cells by kaempferol via _cAMP/PKA-dependent pathway, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP16.

Researcher : Leung SWS

Project Title:	The role of calcium calmodulin dependent protein kinase II in the regulation of endothelial-derived hyperpolarizing factor-mediated responses
Investigator(s):	Leung SWS, Man RYK
Department:	Pharmacology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2005

Abstract:

Introduction: The endothelium plays an important role in the regulation of the vascular tone, through the release of many vasoactive substances such as nitric oxide, endothelial-derived hyperpolarizing factor (EDHF), prostaglandins and endothelin-1. Of the endothelial-derived relaxing factors, EDHF is the least characterized. Several putative EDHF candidates are the metabolites of arachidonic acid, including anandamide [1], epoxyeicosatrienoic acids (EET) [2] and hydrogen peroxide (H2O2) [3]. Anandamide is the metabolic product of the action of amidohydrolase on arachidonic acid, whereas both EET and H2O2 are generated by cytochrome P450 epoxygenase. It appears that the release of EDHF, like other vasoactive agents, can be activated by an increase of intracellular concentration of free calcium, which then associates with calmodulin in the endothelial cells [4]. While most research effort has been devoted to identify the chemical entity and the mechanisms of action of EDHF, little is known regarding the regulation of the production of EDHF. Objectives: In view of the involvement of the calcium/calmodulin complex in the stimulation of the production of EDHF, it is hypothesized that calcium/calmodulin dependent protein kinase II (CaMK II) plays a role in regulating the release of EDHF. Therefore, the aim of the present study is to investigate the contributions of CaMK II to the regulation of EDHF-mediated relaxation. References: 1. Randall MD, Alexander SP, Bennett T et al., Biochem Biophys Res Commun 1996; 229: 114-120. 2. Campbell WB, Gebremedhin D, Pratt PF et al., Circ Res 1996; 78: 415-423. 3. Matoba T, Shimokawa H, Nakashima M et al., J Clin Invest 2000; 106: 1521-1530. 4. Illiano S, Nagao T, Vanhoutte PM. Br J Pharmacol 1992; 107: 387-392.

Project Title:	The role of extracellular signal-regulated kinases in the pathophysiology of hypertension
Investigator(s):	Leung SWS
Department:	Pharmacology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2005
Completion Date:	06/2006

Abstract:

N/A

Project Title:	The role of extracellular signal-regulated kinases in the pathophysiology of hypertension
Investigator(s):	Leung SWS, Man RYK
Department:	Pharmacology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2006

Abstract:

Introduction: Hypertension is a disease characterized by spontaneous arterial tone, enhanced contraction, reduced relaxation and vascular smooth muscle hypertrophy. If not managed properly, hypertension is associated with vascular complications such as arteriosclerotic lesions and further increase in arterial pressure. Elevation of blood pressure is associated with increased sheer stress [1], which is generated by the streaming blood on the endothelial layer. Acute increases in sheer stress in rat arteries in vitro and in vivo lead to activation of extracellular signal-regulated kinase-1/2 (ERK 1/2) [2-4], and this, in turn, results in vascular smooth muscle cell growth [5]. In addition to the involvement of vascular remodeling, recent studies suggest that ERK 1/2 modulate vascular contraction and relaxation. These latter actions of ERK 1/2 may also play an important role in the progress of vascular disease in hypertension [6-10]. Hypothesis: We hypothesize that the activity of ERK 1/2 in endothelial cells is increased in hypertension and this changes in endothelial ERK1/2 activity is related to changes in the vascular responses in this pathological state. Purpose of the proposed investigation: Endothelial ERK 1/2 is likely to relate to changes in the vascular responses in hypertension, as suggested by its association with prostaglandin synthesis, eNOS activity and EDHF level. While ERK 1/2 has been implicated in smooth muscle cell hypertrophy during arterial remodeling in hypertension, the involvement of ERK 1/2 in endothelial dysfunction and hence the pathogenesis of hypertension has not been clearly examined. Therefore, in this proposal, we sought to use multiple approaches to study the relationship between endothelial ERK 1/2 and hypertension in more details. Key issues and problem addressed: i. The importance of endothelial ERK 1/2 in the regulation of the vascular tone will be examined under physiological condition, as well as in hypertensive state. ii. The expression, phosphorylation and activity of ERK 1/2 in the endothelium will be studied in healthy and hypertensive animals, and the results will be correlated to the degree of endothelial dysfunction in hypertension. Reference [1] Thubrikar MJ, Robicsek F. Ann Thorac Surg 1995; 59: 1594-1603. [2] Xu Q, Liu Y, Gorospe M, et al. J Clin Invest 1996; 97: 508-514. [3] Birukov KG, Lehoux S, Birukova AA, et al. Circ Res 1997; 81: 895-903. [4] Pyles JM, March KL, Franklin M, et al. Circ Res 1997; 81: 904-910. [5] Numaguchi K, Eguchi S, Yamakawa T, et al. Circ Res 1999; 85: 5-11. [6] Bhattacharya S, Patel R, Sen N, et al. Am J Physiol 2001; 280: L1049-1056. [7] Brandes RP, Popp R, Ott G. Br J Pharmacol 2002; 136: 709-716. [8] Bernier SG, Haldar S, Michel T. J Biol Chem 2000; 275: 30707-30715. [9] Schmidt K, Gibraeil HD, Mayer B. Biochem Pharmacol 2002; 63: 1137-1142 [10] Fleming I, Fisslthaler B, Dimmeler S, et al. Circ Res 2001; 88: E68-E75.

List of Research Outputs

Chan M.L.Y., Keung W.W.Y., Leung S.W.S., Che C.M. and Man R.Y.K., Role of the nitric oxide signaling cascade in the biological actions of traditional chinese medicine, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Leung S.W.S., Quan A., Lao T.T. and Man R.Y.K., Efficacy of different vasodilators on human umbilical arterial smooth muscle under normal and reducted oxygen conditions, Early Human Development . 2005, (2006) 82;: 457-462.

Leung S.W.S., Lee M.Y.K., Xu Y. and Man R.Y.K., Phytoestrogens and vascular responsiveness, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006. 2006.

Man R.Y.K., Leung S.W.S., Teoh H., Quan A., Keung W.W.Y. and Lee M.Y.K., Phytoestrogens and cardiovascular disorders, Pathophysiology of Cardiovascular Disease. Kluwer Academic Publishers, 2005, IV: 513-524.

Ng W.W.H., Man R.Y.K., Choy P.C. and Leung S.W.S., Genistein induces nitric oxide release from vascular endothelial cells, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Xu Y., Yeung K.Y., Man R.Y.K. and Leung S.W.S., Kaempferol enhances endothelium-independent and dependent relaxation in the porcine coronary artery, Molecular and Cellular Biochemistry. 2005, 2006;287: 61-67.

Xu Y., Leung S.W.S., Leung G.P.H. and Man R.Y.K., Kaempferol, a compound from chinese medicine, potentiated relaxation in porcine arteries via cAMP pathway and activation of potassium channel, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 338.

Xu Y., Leung S.W.S., Leung G.P.H., Vanhoutte P.M.G.R. and Man R.Y.K., Structure activity relationship of phytoestrogens and their modulation of relaxing responses in porcine coronary arteries, 2006 AAPS National Biotechnology Conference, Boston, MA, June 18-21, 2006 . 2006, W3028: 112.

Xu Y.C., Leung G.P.H., Leung S.W.S., Vanhoutte P.M.G.R. and Man R.Y.K., Stimulation of large-conductance calcium-activated potassium channels in cultured endothelial cells by kaempferol via _cAMP/PKA-dependent pathway, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP16.

Researcher : Li RWS

List of Research Outputs

Li R.W.S., Man R.Y.K., Tse C.M. and Leung G.P.H., Effects of calcium antagonists on human equilibrative nucleoside transporters, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 361.

Researcher : Man GSK

List of Research Outputs

Michel F.S.J., Man G.S.K., Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Age, hypertension and nitric oxide synthase (NOS) inhibition angment endothelium-derived contracting factor (EDCF) in the rat renal artery, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 174.

Researcher : Man RYK

Project Title:	Defining the bioactive components of Shenmai injection and their mechanisms of action
Investigator(s):	Man RYK, O K, Siow YL, Li W.D., Zhu D.Y.
Department:	Pharmacology
Source(s) of Funding:	Matching Grant for Joint Research
Start Date:	05/2002
Completion Date:	07/2005

Abstract:

To obtain a definitive chemical fingerprint of Shen Mai injection; to isolate, identify, and associate the major bioactive components and their structures to measureable biological activities deemed beneficial to cardiovascular disease.

Project Title:	Modulatory effects of 17β-estradiol on the vascular responses of rat isolated mesenteric and pulmonary arteries
Investigator(s):	Man RYK
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2005

Abstract:

To study the acute vascular effects of 17β-estradiol in male as well as female rats. In addition, the modulatory actions of long-term treatment of 17β-estradiol on arterial relaxation will be examined in ovariectomized or sham-operated rats. The possible signal transduction pathway underlying the vascular effects of 17β-estradiol will also be investigated.

Project Title:	Vascular function and structure activity relationship of flavonoids
Investigator(s):	Man RYK, Leung SWS
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004
Completion Date:	04/2006

Abstract:

To compare the vascular effect of various flavonoids and to study the structure activity relationship of these flavonoids.

Project Title:	Cloning and characterization of phytoestrogen receptors in vascular cells
Investigator(s):	Man RYK, Leung GPH, Leung SWS
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	12/2005

Abstract:

Epidemiological studies have demonstrated that women are less vulnerable to cardiovascular diseases during their pre-menopausal years. However, the incidence of cardiovascular diseases increases after menopause, stating the importance of female hormone in cardiovascular protective effects. Estrogen replacement therapy has been demonstrated to be beneficial to the cardiovascular system. For instance, it can decrease low-density lipoprotein and increase high density lipoprotein. Besides, estrogen shows antioxidant and vasodilatory effects. Unfortunately, the actual benefit of estrogen therapy is questionable because it increases the risk of endometrial and breast cancer. The hormonal effects also make estrogen not useful in male. Phytoestrogens are an alternative. Soybean is rich in phytoestrogens. A number of studies have suggested the beneficial effects of soy-rich diet in lowering the mobility and mortality of cardiovascular diseases. Same as estrogen, soybean products reduce the blood levels of low density lipoprotein and triglyceride. Our laboratory has also proved that genistein, a phytoestrogen, enhances the endothelium-independent relaxation in coronary artery. These effects may contribute to the prevention of cardiovascular disorders. Most importantly, although genistein is structurally similar to estrogen, its affinity to the genomic estrogen receptors is 100-1000 times less than the estrogen. Therefore, it is assumed that phytoestrogens, while producing beneficial cardiovascular effects, should show negligible hormonal effects. Our tissue bath studies have demonstrated the vasodilatory effects of several other phytoestrogens. Interestingly, the actions of all those phytoestrogens occur within 30 minutes. It is too rapid to have been attributed to the genomic action. This notion is supported by the lack of effect of actinomycin D, a DNA transcription inhibitor, or cycloheximide, a mRNA translation inhibitor, to block the vascular effects of phytoestrogens. Moreover, the inability of the classic estrogen receptor antagonist, ICI 182,780, to block the effects of phytoestrogens further indicates that phytoestrogen may act on receptors which are distinct from the classic genomic estrogen receptors. In this grant application, we propose to clone and characterize phytoestrogen receptors in vascular cells. In aim I, we will clone phytoestrogen receptors. In aim II, we will express the cloned phytoestrogen receptors in COS-7 cells. We will study their molecular sizes, cellular localization and affinities to phytoestrogens and estrogen. If they show high affinities to phytoestrogen, we will study if they are linked to cAMP or cGMP production in vascular smooth muscle. In aim III, we will determine the tissue distribution of phytoestrogen receptors.

List of Research Outputs

Chan M.L.Y., Keung W.W.Y., Leung S.W.S., Che C.M. and Man R.Y.K., Role of the nitric oxide signaling cascade in the biological actions of traditional chinese medicine, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Keung W.Y.W., Vanhoutte P.M.G.R. and Man R.Y.K., Nongenomic responses to 17b-estradiol in male rat mesenteric arteries abolish intrinsic gender differences in vascular responses, British Journal of Pharmacology. Nature, 2005, 146: 1148-1155.

Kwok Y., Ng J.K.F., Li C.F., Lam C.C.K. and Man R.Y.K., A prospective, randomized, double blind, placebo-controlled study of the platelet and global hemostatic effects of Ganoderma Lucideum (Ling-Zhi) in healthy volunteers, Anaesthesia & Analgesia. USA, 2005, 101: 423-426.

Lau C.T. and Man R.Y.K., Endothelium-independent relaxation enhanced by isoflavone metabolite equol, Acta Pharmacologica Sinica - 15th world Congress of Pharmacology July 2-7, 2006 beijing, China. 2006, 2006 July; Suppl 1: 171.

Lau C.T. and Man R.Y.K., Isoflavone metabolite equol enhanced endothelium-independent relaxation, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. OP14.

Lee M.Y.K., Tse H.F., Man R.Y.K. and Vanhoutte P.M.G.R., Genomic changes in cultured porcine regenerated coronary endothelial cells, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 21.

Lee M.Y.K., Holmskov U., Man R.Y.K. and Vanhoutte P.M.G.R., Reduced up regulation of SP-D in response to TNF-a in senescent endothelial cells, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 171.

Lee Y.K.M., Tse H.F., Man R.Y.K. and Vanhoutte P.M.G.R., Genomic changes in culltured porcine regenrated coronary endothelial cells, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP15.

Leung G.P.H., Man R.Y.K. and Tse C.M., Effect of thiazolidinediones on equilibrative nucleoside transporter-1 in human aortic smooth muscle cells. , Biochemical Pharmacology. 2005, 70: 355-362.

Leung S.W.S., Quan A., Lao T.T. and Man R.Y.K., Efficacy of different vasodilators on human umbilical arterial smooth muscle under normal and reducted oxygen conditions, Early Human Development . 2005, (2006) 82;: 457-462.

Leung S.W.S., Lee M.Y.K., Xu Y. and Man R.Y.K., Phytoestrogens and vascular responsiveness, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006. 2006.

Li R.W.S., Man R.Y.K., Tse C.M. and Leung G.P.H., Effects of calcium antagonists on human equilibrative nucleoside transporters, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 361.

Man R.Y.K., Leung S.W.S., Teoh H., Quan A., Keung W.W.Y. and Lee M.Y.K., Phytoestrogens and cardiovascular disorders, Pathophysiology of Cardiovascular Disease. Kluwer Academic Publishers, 2005, IV: 513-524.

Michel F.S.J., Man G.S.K., Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Age, hypertension and nitric oxide synthase (NOS) inhibition angment endothelium-derived contracting factor (EDCF) in the rat renal artery, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 174.

Ng J.K.F., Vanhoutte P.M.G.R. and Man R.Y.K., The role of pertussis toxin pathways in EDHF mediated relaxation in porcine coronary arteries, Journal of Vascular Research. Switzerland, 2005, 42 Suppl 1: 12: S2010.

Ng W.W.H., Man R.Y.K., Choy P.C. and Leung S.W.S., Genistein induces nitric oxide release from vascular endothelial cells, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelial dysfunction in the femoral artery from one-year old rats, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 137.

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Oxygen-derived free radicals mediate endothelium-derived contractions in the femoral artery from streptozotocin-treated rats, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7,2006 Beijing, China. 2006, 2006 July; Suppl 1: 170.

Shi Y., Feletou M., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., The calcium honophore A23187 induces endothelium-dependent contractions in femoral arteries from rats with streptozotocin-induced diabetes, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 51.

Tang H.C., Huang F.Y., So K.F., Man R.Y.K. and Vanhoutte P.M.G.R., Agonists causing endothelium-dependent contractions increase calcium in endothelial cells of the aorta of the spontaneously hypertensive rat, Journal of the Hong Kong College of Cardiology. 2005, 13(2): 120 OP92.

Tang H.C., Ku D.D., Tipoe G.L., Feletou M., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelium-dependent contractions occur in the aorta of wild-type and COX2^-/- knockout but not COX1^-/- knockout mice, Journal of Cardiovascular Pharmacology. 2005, 46(6): 761-765.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Prostanoid synthases and receptors in aorta of spontaneously hypertensive rat, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 138.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Real-time quantitative PCR study of genes involved in the generation of endothelium-depedent contractions in the aorta of the spontaneously hypertensive rat, FASEB Journal. 2006, 20(4): A290-A291 Part 1.

Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Spontaneous conference in the aorta of the spontaneously hypertensive rats, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; suppl 1: 132.

Tang J., Feng Y., Tong Y., Jia R., Yap M.M.L. and Man R.Y.K., A comparative study on three species of Radix et Rhizoma Rhei , Conference on the standardization of Chinese Medicines. Hong Kong, 2005, 99-103.

Xu Y., Yeung K.Y., Man R.Y.K. and Leung S.W.S., Kaempferol enhances endothelium-independent and dependent relaxation in the porcine coronary artery, Molecular and Cellular Biochemistry. 2005, 2006;287: 61-67.

Xu Y., Leung S.W.S., Leung G.P.H. and Man R.Y.K., Kaempferol, a compound from chinese medicine, potentiated relaxation in porcine arteries via cAMP pathway and activation of potassium channel, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 338.

Xu Y., Leung S.W.S., Leung G.P.H., Vanhoutte P.M.G.R. and Man R.Y.K., Structure activity relationship of phytoestrogens and their modulation of relaxing responses in porcine coronary arteries, 2006 AAPS National Biotechnology Conference, Boston, MA, June 18-21, 2006 . 2006, W3028: 112.

Xu Y.C., Leung G.P.H., Leung S.W.S., Vanhoutte P.M.G.R. and Man R.Y.K., Stimulation of large-conductance calcium-activated potassium channels in cultured endothelial cells by kaempferol via _cAMP/PKA-dependent pathway, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP16.

Researcher : Michel FSJ

List of Research Outputs

Michel F.S.J., Man G.S.K., Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Age, hypertension and nitric oxide synthase (NOS) inhibition angment endothelium-derived contracting factor (EDCF) in the rat renal artery, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 174.

Michel F.S.J. and Vanhoutte P.M.G.R., Endothelial dysfunction: a multifaceted disorder, Am J Physiol Heart Circ Physiol . 2006, 291: H985-H1002.

Researcher : Ng WWH

List of Research Outputs

Ng W.W.H., Man R.Y.K., Choy P.C. and Leung S.W.S., Genistein induces nitric oxide release from vascular endothelial cells, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 341.

Researcher : O K

Project Title:	Mechanisms of enhanced monocyte binding to vascular endothelium in hyperhomocysteinemia
Investigator(s):	O K
Department:	Pharmacology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2002
Completion Date:	08/2005

Abstract:

The project aims to investigate the effect of elevated blood homocysteine (Hcy) levels on monocyte binding to aortic endothelium in dietary-induced hyperhomocysteinemic rats as well as the underlying mechanisms. Specifically, efforts will be made to investigate the mechanisms of MCP-1 expression in endothelium and CCR2 expression in peripheral blood moncytes.

List of Research Outputs

Researcher : Shi Y

List of Research Outputs

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelial dysfunction in the femoral artery from one-year old rats, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 137.

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Oxygen-derived free radicals mediate endothelium-derived contractions in the femoral artery from streptozotocin-treated rats, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7,2006 Beijing, China. 2006, 2006 July; Suppl 1: 170.

Shi Y., Feletou M., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., The calcium honophore A23187 induces endothelium-dependent contractions in femoral arteries from rats with streptozotocin-induced diabetes, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 51.

Researcher : Shin VY

List of Research Outputs

Cho C.H. and Shin V.Y., Nicotine and gastric cancer, International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 16.

Hui K.C., Wu K.K., Shin V.Y., Cho C.H. and So H.L., Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice , International Journal of Medical Sciences. 2006, 2006;3: 1-6.

Lam K.Y., Wu K.K., Wong P.S.H., Tai K.K., Shin V.Y., So H.L. and Cho C.H., Lactobacillus GG enhances gastric ulcer healing via attenuation of cellular apoptosis and promotion of angiogenesis and cell proliferation, International Symposium on Translational Research, Trondheim, Norway December 9-10, 2005. 12.

Shin V.Y., Wu K.K., Chu K.M., Wong P.S.H., Lam K.Y., Tai K.K., Koo M.W.L. and Cho C.H., Nicotine induces cyclooxygenase-2 and vascular endothelial growth factor receptor-2 in association with tumor-associated invasion and angiogenesis in gastric cancer, Mol Cancer Res. 2005, 3(11): 607-15.

Wong P.S.H., Wu K.K., Tai K.K., Lam K.Y., Shin V.Y., So H.L. and Cho C.H., Beta-adrenoceptors mediate colon cancer cell proliferation induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 22.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K., Yang Y., Tai K.K., Wong H.P.S., Lam K.Y., Shin V.Y. and Cho C.H., The antimicrobial peptide cathelicidin promotes gastric ulcer healing in rats, Supplement to Gastroenterology - Digestive Disease Week and the 107th annual Meeting of the American Gastroenterological Association Institute May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-543.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Ye Y., Wu K.K., Shin V.Y. and Cho C.H., A mechanistic study of colon cancer growth promoted by cigarette smoke extract, European Journal of Pharmacology. 2005, 519 (2005): 52-57.

Researcher : Shin VY

List of Research Outputs

Cho C.H. and Shin V.Y., Nicotine and gastric cancer, International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 16.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Ye Y., Wu K.K., Shin V.Y. and Cho C.H., A mechanistic study of colon cancer growth promoted by cigarette smoke extract, European Journal of Pharmacology. 2005, 519 (2005): 52-57.

Researcher : So HL

List of Research Outputs

Hui K.C., Wu K.K., Shin V.Y., Cho C.H. and So H.L., Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice , International Journal of Medical Sciences. 2006, 2006;3: 1-6.

Kopaniszen M., Tai K.K., Lam K.Y., So H.L., Cho C.H. and Koo M.W.L., The effects of green tea catechin on DNBS-induced ulcerative colitis in mice, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 73(suppl 2): 37.

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Lam K.Y., Wu K.K., Wong P.S.H., Tai K.K., Shin V.Y., So H.L. and Cho C.H., Lactobacillus GG enhances gastric ulcer healing via attenuation of cellular apoptosis and promotion of angiogenesis and cell proliferation, International Symposium on Translational Research, Trondheim, Norway December 9-10, 2005. 12.

Li G., Xia H.H.X., Chen M., Gu Q., Wang J., Peng J., Chan O.O., Cho C.H., So H.L., Lam S.K., Hu P.J., Liang Y.J., Lin H., Berg D.E., Feng Z.H., Langenback R. and Wong B.C.Y., Effects of Cyclooxygenase-1 and -2 Gene Disruption on Helicobacter pylori-Induced Inflammation, Journal of Infectious Diseases. 2006, 193(7): 1037-1046.

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Wong P.S.H., Wu K.K., Tai K.K., Lam K.Y., Shin V.Y., So H.L. and Cho C.H., Beta-adrenoceptors mediate colon cancer cell proliferation induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 22.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Tai KK

List of Research Outputs

Kopaniszen M., Tai K.K., Lam K.Y., So H.L., Cho C.H. and Koo M.W.L., The effects of green tea catechin on DNBS-induced ulcerative colitis in mice, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 73(suppl 2): 37.

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Lam K.Y., Wu K.K., Wong P.S.H., Tai K.K., Shin V.Y., So H.L. and Cho C.H., Lactobacillus GG enhances gastric ulcer healing via attenuation of cellular apoptosis and promotion of angiogenesis and cell proliferation, International Symposium on Translational Research, Trondheim, Norway December 9-10, 2005. 12.

Shin V.Y., Wu K.K., Chu K.M., Wong P.S.H., Lam K.Y., Tai K.K., Koo M.W.L. and Cho C.H., Nicotine induces cyclooxygenase-2 and vascular endothelial growth factor receptor-2 in association with tumor-associated invasion and angiogenesis in gastric cancer, Mol Cancer Res. 2005, 3(11): 607-15.

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

Tai K.K., Wu K.K. and Cho C.H., The role of cathelicidins in inflammation and wound healing, Ulcer Research. 2006, 33:1-2006: 54-60.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Wong P.S.H., Wu K.K., Tai K.K., Lam K.Y., Shin V.Y., So H.L. and Cho C.H., Beta-adrenoceptors mediate colon cancer cell proliferation induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 22.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K., Yang Y., Tai K.K., Wong H.P.S., Lam K.Y., Shin V.Y. and Cho C.H., The antimicrobial peptide cathelicidin promotes gastric ulcer healing in rats, Supplement to Gastroenterology - Digestive Disease Week and the 107th annual Meeting of the American Gastroenterological Association Institute May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-543.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Tai KK

List of Research Outputs

Tai K.K., Wu K.K. and Cho C.H., The role of cathelicidins in inflammation and wound healing, Ulcer Research. 2006, 33:1-2006: 54-60.

Researcher : Tang HC

List of Research Outputs

Michel F.S.J., Man G.S.K., Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Age, hypertension and nitric oxide synthase (NOS) inhibition angment endothelium-derived contracting factor (EDCF) in the rat renal artery, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 174.

Tang H.C., Huang F.Y., So K.F., Man R.Y.K. and Vanhoutte P.M.G.R., Agonists causing endothelium-dependent contractions increase calcium in endothelial cells of the aorta of the spontaneously hypertensive rat, Journal of the Hong Kong College of Cardiology. 2005, 13(2): 120 OP92.

Tang H.C., Ku D.D., Tipoe G.L., Feletou M., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelium-dependent contractions occur in the aorta of wild-type and COX2^-/- knockout but not COX1^-/- knockout mice, Journal of Cardiovascular Pharmacology. 2005, 46(6): 761-765.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Prostanoid synthases and receptors in aorta of spontaneously hypertensive rat, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 138.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Real-time quantitative PCR study of genes involved in the generation of endothelium-depedent contractions in the aorta of the spontaneously hypertensive rat, FASEB Journal. 2006, 20(4): A290-A291 Part 1.

Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Spontaneous conference in the aorta of the spontaneously hypertensive rats, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; suppl 1: 132.

Researcher : Tang HC

List of Research Outputs

Researcher : Vanhoutte PMGR

Project Title:	Endothelial dysfunction in diabetes: role of endothelium-derived contracting factor
Investigator(s):	Vanhoutte PMGR
Department:	Pharmacology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	08/2003
Completion Date:	07/2005

Abstract:

To examine five hypotheses: a) the lack of NO favors endothelium-dependent contractions: b) the endothelium of diabetic arteries releases more vasoconstrictor breakdown products of arachidonic acid: c) endothelium-dependent contractions are due to a diffusible factor (EDCF); d) oxygen-free radicals play a key role in EDCF-mediated responses: and e) high glucose mimicks the impact of diabetes on endothelial function. These studies should yield a better understanding of the mechanisms(s) underlying diabetic endothelial dysfunction, and hence help to improve the prevention and/or therapy of one of the most feared complications of the disease.

Project Title:	Endothelial dysfunction in diabetes: role of endothelium-derived contracting factor
Investigator(s):	Vanhoutte PMGR
Department:	Pharmacology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	08/2003
Completion Date:	07/2005

Abstract:

N/A

Project Title:	EDCF in spontaneous hypertension
Investigator(s):	Vanhoutte PMGR
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2005

Abstract:

To explore the exact mechanism(s) underlying, and the importance of, endothelium-dependent contractions in large and small arteries of the spontaneously hypertensive rat, and to study blood vessels of the mouse in order to be able to use genetically modified animals.

Project Title:	Genomic changes underlying the phenotypic alterations of regenerated endothelial cells
Investigator(s):	Vanhoutte PMGR, Man RYK, Vilaine JP
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004
Completion Date:	04/2006

Abstract:

To define the changes of regenerated endothelial cells at the genomic level which could explain the phenotypic alterations that underlie their dysfunction.

Project Title:	Genomic Changes in Regenerated Endothelium
Investigator(s):	Vanhoutte PMGR, Mak WW, Tse HF
Department:	Pharmacology
Source(s) of Funding:	Small Project Funding
Start Date:	08/2005
Completion Date:	07/2006

Abstract:

Endothelium-dependent responses of the vasculature are due to the release of diffusible substances [Endothelium-derived relaxing (EDRF) and contracting (EDCF) factors] from the endothelial cells (1, 2). Over the last twenty five years, the laboratory of the PI has examined how the production by endothelial cells of such factors underlies moment-to-moment changes in the tone of the surrounding vascular smooth muscle cells, and how the inability of the endothelial cells to do so properly eventually initiates atherosclerosis where the relative absence of nitric oxide (NO) plays a key role. NO is formed from L-arginine, by endothelial NO-synthase (eNOS). NO diffuses to the underlying smooth muscle cells to stimulate soluble guanylate cyclase which accelerates the formation of cyclic GMP. The latter in turn inhibits the contractile process. NO is released also in the lumen and inhibits the adhesion of platelets and white cells to the endothelium and platelet aggregation. It also inhibits the growth of the vascular smooth muscle cells and prevents the production of adhesion molecules (3-5). The release of NO is triggered by activation of receptors on the endothelial cell membrane. The triggers include autacoids (e.g. bradykinin) and products formed during platelet aggregation; serotonin, ADP and thrombin. The cell membrane receptors for these substances are coupled to the activation of eNOS by different families of G-proteins. Thus, in coronary arteries, adrenergic receptors, serotonin receptors and thrombin receptors are coupled to pertussis toxin-sensitive Gi-proteins. By contrast the receptors for ADP or bradykinin are not (see 6). The activation of eNOS by bradykinin involves low molecular weight G-proteins of the Rho-family (7). In coronary and cerebral arteries, aggregating platelets induce endothelium-dependent relaxations, and the presence of a healthy endothelium inhibits the constriction induced by the platelet products (thromboxane A2 and serotonin). Serotonin, acting on 5-HT1D-serotonin receptors, plays the major role in this response, while ADP, activating P2y-purinoceptors, contributes little. The release of NO, both toward the underlying smooth muscle and at the interface with the blood, in response to thrombin and platelet-derived serotonin is pivotal for the protective role played by the healthy endothelium against the platelet attack (see 3-5).In the course of ageing, and in several types of vascular disease and hypertension, the endothelial cells become dysfunctional (see 3-6). This dysfunction is evidenced by an impairment in endothelium-dependent relaxations, due mainly to a reduced availability of NO. Indeed, the normal aging process induces a turn-over (apoptotic death, desquamation followed by regeneration) of endothelial cells. Unfortunately, regenerated endothelial cells have lost part of the ability to release NO, in response to platelet aggregation (8,9), because they respond minimally to serotonin, and other substances using the Gi-protein dependent pathway controlling the release of NO. The Gi proteins are present, but exhibit a reduced activity (10). The loss of the pertussis toxin-sensitive response is selective, and it does not apply, at least initially, to endothelium-dependent responses mediated by Gq-coupling proteins, in particular that to bradykinin (6-9). It is caused by the greater accumulation of oxidized low density lipoproteins (LDL) by the regenerated endothelial cells (10,11). Hypercholesterolemia also impairs endothelium-dependent relaxations (e.g. 9, 12, 13). In the initial phase of the atherosclerotic process, the endothelial dysfunction is limited to the pertussis toxin-sensitive, Gi protein-dependent pathway. Thus, the ability of regenerated endothelial cells, chronically exposed to high cholesterol levels, to ADP-ribosylate pertussis toxin is reduced (14). Hence, in coronary arteries from hypercholesterolemic pigs, the endothelium-dependent relaxations serotonin; 2-adrenergic agonists, aggregating platelets, or thrombin are depressed while those induced by ADP and bradykinin are maintained (12, 13). Oxidized LDL induces, in vitro, a similar selective endothelial dysfunction while at higher concentrations it inhibits also endothelium-dependent relaxations in response to non-Gi dependent stimulations (e.g.15 ;). The molecular basis underlying the dysfunction of regenerated endothelial cells is unknown. To judge from work comparing primary cell cultures derived from areas of the same coronary arteries covered with either native or regenerated endothelium, the latter has underwent phenotypic changes that include: a) morphological changes (appearance of giant ant multinucleated cells) indicating accelerated senescence; b) greater production of oxygen-derived free radicals; c) enhanced apoptosis; d) accelerated uptake of modified LDL despite normal affinity of the LOX-1 receptors on the cell surface; and e) exaggerated accumulation of oxidized forms of Apoprotein-B-100 ( 10, 11, 16). The proposed research will attempt to define the changes of regenerated endothelial cells at the genomic level which could explain the phenotypic alterations that underlie their dysfunction. REFERENCES: 1) Furchgott RF, Zawadzki JV. Nature 1980; 288: 373-376; 2) Furchgott RF, Vanhoutte PM. FASEB J 1989 ; 3: 2007-2018; 3)Vanhoutte PM In: Panza JA and Cannon III RO, editors. Futura Publishing Co, Inc, Armonk, NY; 1999; 79-95; 4) Vanhoutte PM. Eur Heart J 2002; 4:A8-A17; 5) Vanhoutte PM. Circulation J. 2003; 67:572-575; 6) Flavahan NA and Vanhoutte PM. Am J Hypertens 1995; 8:28S-41S; 7) Shibano T and Vanhoutte PM. Acta Pharmacologica Sinica 2003; 24:1070-1076. 8) Shimokawa H, Aarhus LL, Vanhoutte PM. Circ Res 1987;61:256-270; 9) Shimokawa H, Flavahan NA, Vanhoutte PM. Circ Res 1989; 65:740-753; 10) Borg-Capra C, Fournet-Bourguignon MP, Janiak P, Villeneuve N, Bidouard JP, Vilaine JP, Vanhoutte PM. Br J Pharmacol 1997; 122:999-1008; 11) Castedo-Delrieu M, Fournet-Bourguignon MP, Bidouard JP, Delescluse I, Leonce S , Vilaine JP, Vanhoutte PM. J Vasc Res 1997 ; 34 (suppl 1) : 10; 12) Shimokawa H, Vanhoutte PM. Circ Res.1989; 64:900-914; 13) Shimokawa H, Flavahan NA, Vanhoutte PM. Circulation 1991; 83:652-660; 14) Shibano T, Codina J, Birnbaumer L, Vanhoutte PM. Am J Physiol 1994; 267:H979-H981; 15) Cox DA and Cohen ML. Pharmacol Rev 1996; 48:3-19; 16) Kennedy, S., Fournet-Bourguignon, M-P., Breugnot, C., Castedo-Delrieu, M., Lesage, L., Reure, H., Briant, C., Leonce, S., Vilaine, J-P. ,Vanhoutte, P.M. J. Vasc. Res. 2003;40:389-398.

List of Research Outputs

Chan Y. .C., Yan X., Laher I., Vanhoutte P.M.G.R. and Huang Y., Does chronic raloxifene treatment affect vascular reactivity in pressurized septal coronary arteries from old hamsters?, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. P25.

Feletou M. and Vanhoutte P.M.G.R., Endothelium-derived hyperpolarizing factor, Arterioscler Thromb Vasc. Biol.. 2006, 2006;26: 1215-1225.

Feletou M. and Vanhoutte P.M.G.R., Is endothelium-derived hyperpolarizing factor a therapeutic drug target?, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 76.

Feletou M. and Vanhoutte P.M.G.R., Prostacyclin:EDRF, EDHF and EDCF, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 125.

Gluais P., Lonchampt M., Morrow J...D..., Vanhoutte P.M.G.R. and Feletou M., Acetylcholine-induced endothelium-dependent contractions in the SHR aorta: the Janus face of prostacyclin, British Journal of Pharmacology. Nature, 2005, 146: 834-845.

Haddy F. .J., Vanhoutte P.M.G.R. and Feletou M., Role of potassium in regulating blood flow and blood pressure, Am J Physiol Regal Integr Comp Physiol. 2005, 290: R546-R552.

Keung W.Y.W., Vanhoutte P.M.G.R. and Man R.Y.K., Nongenomic responses to 17b-estradiol in male rat mesenteric arteries abolish intrinsic gender differences in vascular responses, British Journal of Pharmacology. Nature, 2005, 146: 1148-1155.

Lee M.Y.K., Tse H.F., Man R.Y.K. and Vanhoutte P.M.G.R., Genomic changes in cultured porcine regenerated coronary endothelial cells, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 21.

Lee M.Y.K., Holmskov U., Man R.Y.K. and Vanhoutte P.M.G.R., Reduced up regulation of SP-D in response to TNF-a in senescent endothelial cells, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 171.

Lee Y.K.M., Tse H.F., Man R.Y.K. and Vanhoutte P.M.G.R., Genomic changes in culltured porcine regenrated coronary endothelial cells, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP15.

Leung H.S., Leung F.P., Yao X., Ko W.H., Chen Z.Y., Vanhoutte P.M.G.R. and Huang Y., Endothelial mediators of the acetylcholine-inducted relaxation of the rat femoral artery, Vascular Pharmacology. 2006, 2006;44: 299-308.

Michel F.S.J., Man G.S.K., Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Age, hypertension and nitric oxide synthase (NOS) inhibition angment endothelium-derived contracting factor (EDCF) in the rat renal artery, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 174.

Michel F.S.J. and Vanhoutte P.M.G.R., Endothelial dysfunction: a multifaceted disorder, Am J Physiol Heart Circ Physiol . 2006, 291: H985-H1002.

Ng J.K.F., Vanhoutte P.M.G.R. and Man R.Y.K., The role of pertussis toxin pathways in EDHF mediated relaxation in porcine coronary arteries, Journal of Vascular Research. Switzerland, 2005, 42 Suppl 1: 12: S2010.

Rasmussen L.E., Vanhoutte P.M.G.R., Jensen B.L. and Skott O., Continuous luminal flow attenuates basal release of NO but angments that of EDHF in the rabbit carotid artery, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 162.

Rasmussen L.E., Vanhoutte P.M.G.R., Jensen B.L. and Skott O., Flow, pressure and vascular responsiveness, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 50.

Schisrning J., Uhrenholt T.R., Vanhoutte P.M.G.R., Jensen B.L. and Skott O., Intercellular calcium signaling and nitric oxide feedback during constriction of rabbit renal arterioles, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposium, Shanghai-Suzhou 2006, 26-29 June 2006. 134.

Schjeming J., Uhrenholt T.R. and Vanhoutte P.M.G.R., Intercellular calcium signaling and nitric oxide feedback during constriction of rabbit renal arteriodes, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 405.

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelial dysfunction in the femoral artery from one-year old rats, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 137.

Shi Y., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., Oxygen-derived free radicals mediate endothelium-derived contractions in the femoral artery from streptozotocin-treated rats, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7,2006 Beijing, China. 2006, 2006 July; Suppl 1: 170.

Shi Y., Feletou M., Ku D.D., Man R.Y.K. and Vanhoutte P.M.G.R., The calcium honophore A23187 induces endothelium-dependent contractions in femoral arteries from rats with streptozotocin-induced diabetes, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 51.

Tang H.C., Huang F.Y., So K.F., Man R.Y.K. and Vanhoutte P.M.G.R., Agonists causing endothelium-dependent contractions increase calcium in endothelial cells of the aorta of the spontaneously hypertensive rat, Journal of the Hong Kong College of Cardiology. 2005, 13(2): 120 OP92.

Tang H.C., Ku D.D., Tipoe G.L., Feletou M., Man R.Y.K. and Vanhoutte P.M.G.R., Endothelium-dependent contractions occur in the aorta of wild-type and COX2^-/- knockout but not COX1^-/- knockout mice, Journal of Cardiovascular Pharmacology. 2005, 46(6): 761-765.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Prostanoid synthases and receptors in aorta of spontaneously hypertensive rat, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006, 26-29 June 2006. 138.

Tang H.C., Leung G.P.H., Lo A.C.Y., Chung S.K., Man R.Y.K. and Vanhoutte P.M.G.R., Real-time quantitative PCR study of genes involved in the generation of endothelium-depedent contractions in the aorta of the spontaneously hypertensive rat, FASEB Journal. 2006, 20(4): A290-A291 Part 1.

Tang H.C., Man R.Y.K. and Vanhoutte P.M.G.R., Spontaneous conference in the aorta of the spontaneously hypertensive rats, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; suppl 1: 132.

Vanhoutte P.M.G.R., Endothelial dysfunction and vascular disease, Acta Pharmacologica Sinica - 15th world Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 37.

Vanhoutte P.M.G.R., Vascular endothelium and aging, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 25.

Wong C.M., Yao X.Q., Au C.L., Tsang S.Y., Fung K.P., Laher I., Vanhoutte P.M.G.R. and Huang Y., Raloxifene prevents endothelial dysfunction in aging ovariectomized female rats, Vascular Biology. 2005, 2006;44: 290-298.

Xu Y., Leung S.W.S., Leung G.P.H., Vanhoutte P.M.G.R. and Man R.Y.K., Structure activity relationship of phytoestrogens and their modulation of relaxing responses in porcine coronary arteries, 2006 AAPS National Biotechnology Conference, Boston, MA, June 18-21, 2006 . 2006, W3028: 112.

Xu Y.C., Leung G.P.H., Leung S.W.S., Vanhoutte P.M.G.R. and Man R.Y.K., Stimulation of large-conductance calcium-activated potassium channels in cultured endothelial cells by kaempferol via _cAMP/PKA-dependent pathway, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP16.

Researcher : Wong HPS

List of Research Outputs

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K., Wong H.P.S., Ye Y. and Cho C.H., Nicotine and Cancer, Alcohol, Tobacco and Cancer. Karger, 2006, 253-267.

Wu K.K., Yang Y., Tai K.K., Wong H.P.S., Lam K.Y., Shin V.Y. and Cho C.H., The antimicrobial peptide cathelicidin promotes gastric ulcer healing in rats, Supplement to Gastroenterology - Digestive Disease Week and the 107th annual Meeting of the American Gastroenterological Association Institute May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-543.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Wong PSH

List of Research Outputs

Lam K.Y., Wu K.K., Wong P.S.H., Tai K.K., Shin V.Y., So H.L. and Cho C.H., Lactobacillus GG enhances gastric ulcer healing via attenuation of cellular apoptosis and promotion of angiogenesis and cell proliferation, International Symposium on Translational Research, Trondheim, Norway December 9-10, 2005. 12.

Shin V.Y., Wu K.K., Chu K.M., Wong P.S.H., Lam K.Y., Tai K.K., Koo M.W.L. and Cho C.H., Nicotine induces cyclooxygenase-2 and vascular endothelial growth factor receptor-2 in association with tumor-associated invasion and angiogenesis in gastric cancer, Mol Cancer Res. 2005, 3(11): 607-15.

Wong P.S.H., Wu K.K., Tai K.K., Lam K.Y., Shin V.Y., So H.L. and Cho C.H., Beta-adrenoceptors mediate colon cancer cell proliferation induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 22.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Researcher : Wu KK

List of Research Outputs

Hui K.C., Wu K.K., Shin V.Y., Cho C.H. and So H.L., Polysaccharides from the root of Angelica sinensis protect bone marrow and gastrointestinal tissues against the cytotoxicity of cyclophosphamide in mice , International Journal of Medical Sciences. 2006, 2006;3: 1-6.

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Lam K.Y., Wu K.K., Wong P.S.H., Tai K.K., Shin V.Y., So H.L. and Cho C.H., Lactobacillus GG enhances gastric ulcer healing via attenuation of cellular apoptosis and promotion of angiogenesis and cell proliferation, International Symposium on Translational Research, Trondheim, Norway December 9-10, 2005. 12.

Shin V.Y., Wu K.K., Chu K.M., Wong P.S.H., Lam K.Y., Tai K.K., Koo M.W.L. and Cho C.H., Nicotine induces cyclooxygenase-2 and vascular endothelial growth factor receptor-2 in association with tumor-associated invasion and angiogenesis in gastric cancer, Mol Cancer Res. 2005, 3(11): 607-15.

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

Tai K.K., Wu K.K. and Cho C.H., The role of cathelicidins in inflammation and wound healing, Ulcer Research. 2006, 33:1-2006: 54-60.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Wong P.S.H., Wu K.K., Tai K.K., Lam K.Y., Shin V.Y., So H.L. and Cho C.H., Beta-adrenoceptors mediate colon cancer cell proliferation induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), International Symposium on Translational Research, Trondheim, Norway, December 9-10, 2005. 22.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K. and Cho C.H., Involvement of K_v1.1 and Na_v1.5 in proliferation of gastric epithelial cells, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan . 2006, 2006;73 (suppl 2): 6.

Wu K.K., Wong H.P.S., Ye Y. and Cho C.H., Nicotine and Cancer, Alcohol, Tobacco and Cancer. Karger, 2006, 253-267.

Wu K.K., Yang Y., Tai K.K., Wong H.P.S., Lam K.Y., Shin V.Y. and Cho C.H., The antimicrobial peptide cathelicidin promotes gastric ulcer healing in rats, Supplement to Gastroenterology - Digestive Disease Week and the 107th annual Meeting of the American Gastroenterological Association Institute May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-543.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Ye Y., Wu K.K., Shin V.Y. and Cho C.H., A mechanistic study of colon cancer growth promoted by cigarette smoke extract, European Journal of Pharmacology. 2005, 519 (2005): 52-57.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Wu KK

List of Research Outputs

Tai K.K., Wu K.K. and Cho C.H., The role of cathelicidins in inflammation and wound healing, Ulcer Research. 2006, 33:1-2006: 54-60.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Wong H.P.S., Ye Y. and Cho C.H., Nicotine and Cancer, Alcohol, Tobacco and Cancer. Karger, 2006, 253-267.

Ye Y., Wu K.K., Shin V.Y. and Cho C.H., A mechanistic study of colon cancer growth promoted by cigarette smoke extract, European Journal of Pharmacology. 2005, 519 (2005): 52-57.

Researcher : Xu Y

List of Research Outputs

Leung S.W.S., Lee M.Y.K., Xu Y. and Man R.Y.K., Phytoestrogens and vascular responsiveness, 11th International Vascular Neuroeffector Mechanisms and Cardiovascular Pharmacology and Medicine Symposia, Shanghai-Suzhou 2006. 2006.

Xu Y., Yeung K.Y., Man R.Y.K. and Leung S.W.S., Kaempferol enhances endothelium-independent and dependent relaxation in the porcine coronary artery, Molecular and Cellular Biochemistry. 2005, 2006;287: 61-67.

Xu Y., Leung S.W.S., Leung G.P.H. and Man R.Y.K., Kaempferol, a compound from chinese medicine, potentiated relaxation in porcine arteries via cAMP pathway and activation of potassium channel, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 338.

Xu Y., Leung S.W.S., Leung G.P.H., Vanhoutte P.M.G.R. and Man R.Y.K., Structure activity relationship of phytoestrogens and their modulation of relaxing responses in porcine coronary arteries, 2006 AAPS National Biotechnology Conference, Boston, MA, June 18-21, 2006 . 2006, W3028: 112.

Researcher : Xu YC

List of Research Outputs

Xu Y.C., Leung G.P.H., Leung S.W.S., Vanhoutte P.M.G.R. and Man R.Y.K., Stimulation of large-conductance calcium-activated potassium channels in cultured endothelial cells by kaempferol via _cAMP/PKA-dependent pathway, International Symposium on Healthy Aging: A Global Challenge for the 21st Century, Hong Kong March 4-5, 2006. 53, OP16.

Researcher : Yang Y

List of Research Outputs

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K., Yang Y., Tai K.K., Wong H.P.S., Lam K.Y., Shin V.Y. and Cho C.H., The antimicrobial peptide cathelicidin promotes gastric ulcer healing in rats, Supplement to Gastroenterology - Digestive Disease Week and the 107th annual Meeting of the American Gastroenterological Association Institute May 20-25, 2006, Los Angeles, CA. 2006, 130:4 Suppl 2: A-543.

Yang Y., Wu K.K., Tai K.K., Wong H.P.S., Lam K.Y., So H.L., Shin V.Y. and Cho C.H., The cationic host defense peptide rCRAMP promotes gastric ulcer healing in rats, The Journal of Pharmacology and Experimental Therapeutics. 2006, 318; No. 2: 547-554.

Researcher : Yap MML

List of Research Outputs

Tang J., Feng Y., Tong Y., Jia R., Yap M.M.L. and Man R.Y.K., A comparative study on three species of Radix et Rhizoma Rhei , Conference on the standardization of Chinese Medicines. Hong Kong, 2005, 99-103.

Researcher : Ye Y

List of Research Outputs

Lam K.Y., Wong H.P.S., Tai K.K., Ye Y., Wu K.K., Law T.Y., So H.L. and Cho C.H., Gastroprotective action of probiotic lactobacillus GG on ethanol-induced gastric lesions, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl:2): 42.

Wong H.P.S., Wu K.K., Lam K.Y., Tai K.K., Ye Y., Law T.Y., So H.L. and Cho C.H., The contributory role of adrenaline in colon cancer growth, Acta Pharmacologica Sinica - 15th World Congress of Pharmacology, July 2-7, 2006 Beijing, China. 2006, 2006 July; Suppl 1: 400.

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Li G.R., Wong H.P.S., Hui K.C., Tai K.K., Lam K.Y., Shin V.Y., Ye Y., Li P., Yang Y., Luo J.C. and Cho C.H., Involvement of K(v)1.1 and Na(v)1.5 in proliferation of gastric epithelial cells, Journal of Cellular Physiology. 2006, 207: 437-444.

Wu K.K., Wong H.P.S., Ye Y. and Cho C.H., Nicotine and Cancer, Alcohol, Tobacco and Cancer. Karger, 2006, 253-267.

Ye Y., Wu K.K., Shin V.Y. and Cho C.H., A mechanistic study of colon cancer growth promoted by cigarette smoke extract, European Journal of Pharmacology. 2005, 519 (2005): 52-57.

Ye Y., Lam K.Y., Tai K.K., Wu K.K., Law T.Y., So H.L., Cho C.H. and Wong H.P.S., Cigarette smoke extracts increased the proliferation of esophageal cancer cells, 12th International Conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, July 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl2): 57.

Researcher : Ye Y

List of Research Outputs

Wu K.K., Shin V.Y., Ye Y., Wong P.S.H., Huang F. .Y., Hui K.C., Lam K.Y. and Cho C.H., Heparin increases human gastric carcinoma cell growth, Anticancer Research. 2005, 26: 439-444.

Wu K.K., Wong H.P.S., Ye Y. and Cho C.H., Nicotine and Cancer, Alcohol, Tobacco and Cancer. Karger, 2006, 253-267.

Ye Y., Wu K.K., Shin V.Y. and Cho C.H., A mechanistic study of colon cancer growth promoted by cigarette smoke extract, European Journal of Pharmacology. 2005, 519 (2005): 52-57.

Researcher : Yeung KY

List of Research Outputs

Xu Y., Yeung K.Y., Man R.Y.K. and Leung S.W.S., Kaempferol enhances endothelium-independent and dependent relaxation in the porcine coronary artery, Molecular and Cellular Biochemistry. 2005, 2006;287: 61-67.

Researcher : Yu L

List of Research Outputs

Tai K.K., Wu K.K., Wong H.P.S., Lam K.Y., Yu L., Law T.Y., So H.L. and Cho C.H., Study of cathalicidin on ulcerative colitis in mice, 12th International conference on Ulcer Research (ICUR) & GI Satellite of IUPHAR 2006, jULY 7-9, 2006, Osaka, Japan. 2006, 2006:73(suppl 2): 21.

-- End of Listing --