DEPT OF ZOOLOGY
| Researcher : Bao J |
| List of Research Outputs |
| Bao J., Corke H. and Sun M., Analysis of genetic diversity and relationships in waxy rice (Oryza sativa L.) using AFLP and ISSR markers, Genetic Resources and Crop Evolution. 2006, 53: 323-330. |
| Bao J., Shen S.Q., Sun M. and Corke H., Analysis of genotypic diversity in the starch physicochemical properties of nonwaxy rice: Apparent amylose content, pasting viscosity and gel texture, Starch. 2006, 58: 259-267. |
| Researcher : Cai Y |
| Project Title: | Investigating structure-activity relationships of natural phenolic antioxidants from traditional Chinese medicinal plants |
| Investigator(s): | Cai Y, Sun M, Corke H |
| Department: | Botany |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Completion Date: | 04/2006 |
| Abstract: |
| To investigate and elucidate the structure-antioxidant activity relationships of representative natural phenolic antioxidants from traditional Chinese medicinal plants associated with anticancer using the improved ABTS⋅+ method (TEAC assay) at the basis of chemical structural analysis. |
| List of Research Outputs |
| Researcher : Chan DKO |
| List of Research Outputs |
| Wan J.M.F., Sit W.H., Lee C.L., Fu H.M.K. and Chan D.K.O., Protection of lethal toxicity of endotoxin by Salvia miltiorrhiza BUNGE is via reduction in tumor necrosis factor alpha release and liver injury, International Immunopharmacology. 2006, 6: 750-758. |
| Researcher : Chan LY |
| List of Research Outputs |
| Chan L.Y., Chen Q., Go A.G.G., Lam E.K.Y. and Li E.T.S., Reduced Adiposity in Bitter Melon (Momordica charantia) Fed Rats Is Associated with Increased Lipid Oxidative Enzyme Activities and Uncoupling Protein Expression., Journal of Nutrition. 2005, 135: 2517-2523. |
| Researcher : Chan MCW |
| Project Title: | Establishment of primary culture of human airway epithelial cells, as an in-vitro model to study human infection of coronavirus/avian influenza virus |
| Investigator(s): | Chan MCW, Peiris JSM |
| Department: | Microbiology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Completion Date: | 04/2006 |
| Abstract: |
| To establish a primary culture of human airway and alveolar epithelial cells, which can act as an in-vitro model to study human infection of coronavirus and avian influenza virus. |
| Project Title: | Primary human lung epithelial cells as an in-vitro model to study the pathogenesis of SARS coronavirus infection |
| Investigator(s): | Chan MCW, Peiris JSM, Tsao GSW |
| Department: | Microbiology |
| Source(s) of Funding: | Research Fund for the Control of Infectious Diseases - Full Grants |
| Start Date: | 01/2006 |
| Abstract: |
| The research objective is to define cytokine, chemokine, SARS functional receptor gene ACE2 and apoptotic related gene expression profile of human lung epithelial cells in response to infection with infectious and inactivated SARS coronavirus and purified viral proteins. |
| List of Research Outputs |
| Researcher : Chan SM |
| Project Title: | Vitellogenesis in the decapod crustacean: analysis of the shrimp vitellogenin gene and the hormonal control of shrimp vitellogenesis |
| Investigator(s): | Chan SM |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2002 |
| Completion Date: | 08/2005 |
| Abstract: |
| The project attempts to: 1) characterize the shrimp vitelogenin; 2) clone the vitellogenin gene and cDNA; 3) study the expression of shrimp vitellogenin; and 4) study the hormonal effects of vitellogenesis. |
| Project Title: | The role of the GATA transcription factor in the regulation of the shrimp eyestalk crustacean hyperglycemic hormone A (CHH-A) and vitellogenin gene expression |
| Investigator(s): | Chan SM |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2003 |
| Abstract: |
| To clone and characterize the GATA-TF from the shrimp; to analyze the expression of the GATA-TF in relation to the reproductive cycle; to investigate the role of GATA-TF in the regulation of CHH-A gene and vitellogenin gene. |
| Project Title: | The role of the GATA transcription factor in the regulation of the shrimp eyestalk crustacean hyperglycemic hormone A (CHH-A) and vitellogenin gene expression |
| Investigator(s): | Chan SM |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 09/2003 |
| Abstract: |
| N/A |
| Project Title: | Functions of the eyestalk CHH family neuropeptide genes in the shrimp: use of RNA interference to silence genes that suppress reproduction |
| Investigator(s): | Chan SM |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 09/2005 |
| Abstract: |
| N/A |
| Project Title: | Functions of the eyestalk CHH family neuropeptide genes in the shrimp: use of RNA interference to silence genes that suppress reproduction |
| Investigator(s): | Chan SM |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2005 |
| Abstract: |
| To prepare double stranded RNA for several potentially useful genes isolated from shrimp; to introduce double stranded RNA to shrimp by various approaches; to monitor the knock down expression of the gene and study the RNAi effects on the expression of other target genes; to perform in vivo injection of dsRNA for genes that suppress gonad and monitor reproductive promotion. |
| List of Research Outputs |
| Chan S.M., Vitellogenin and vitellogenesis in decapod crustaceans: knowledge from proteomic and molecular studies, SICB Annual Meeting January 4-8, 2006, Buena Vista Palace Resort and Spa in the Walt Disney World Resort, Orlando, FL. 2006. |
| Jian X.F., Lu L., Chen Y.G., Chan S.M. and He J.G., Comparison of a novel in situ polymerase chain reaction (ISPCR) method to other methods for white spot syndrome virus (WSSV) detection in Penaeus vannamei, NCBI, National Library of Medicine and the National Institutes of Health. 2005, 67(1-2): 171-6. |
| Ma H.T. and Chan S.M., Characterization of the C-type lectin cDNA from the shrimps Penaeus monodon and Litopenaeus vannamei, SICB Annual Meeting January 4-8, 2006 Buena Vista Palace Resort and Spa in the Walt Disney World Resort Orlando, FL. 2006. |
| Tiu H.K., Hui J.H.L., He J.G., Tobe S.S. and Chan S.M., Characterization of Vitellogenin in the Shrimp Metapenaeus ensis: Expression Studies and Hormonal Regulation of MeVg1 Transcription In Vitro, Molecular Reproduction and Development. 2006, 73: 424-436. |
| Tiu H.K., Hui J.H.L., Mak A.S.C., He J.G. and Chan S.M., Equal contribution of hepatopancreas and ovary to the production of vitellogenin (PmVg1) transcripts in the tiger shrimp, Penaeus monodon, Aquaculture. 2006, 254: 666-674. |
| Researcher : Chan WL |
| List of Research Outputs |
| Chan W.L., Lung S.C. and Lim B.L., Properties of beta-propeller phytase expressed in transgenic tobacco , Protein expression and purification. 2005, 46: 100-106. |
| Researcher : Chen Q |
| List of Research Outputs |
| Chan L.Y., Chen Q., Go A.G.G., Lam E.K.Y. and Li E.T.S., Reduced Adiposity in Bitter Melon (Momordica charantia) Fed Rats Is Associated with Increased Lipid Oxidative Enzyme Activities and Uncoupling Protein Expression., Journal of Nutrition. 2005, 135: 2517-2523. |
| Researcher : Cheng CW |
| List of Research Outputs |
| Cheng C.W. and Lim B.L., Beta-propeller phytases in the aquatic environment, Archives in Microbiology. 2005, 185: 1-13. |
| Researcher : Cheng CY |
| List of Research Outputs |
| Xia W., Mruk D.D., Lee W.W.M. and Cheng C.Y., Cytokines And Junction Restructuring During Spermatogenesis – A Lesson To Learn From The Testis. , Cytokine & Growth Factor Reviews . 2005, 16: 469-493. |
| Xia W., Wong C.H., Lee P.Y., Lee W.W.M. and Cheng C.Y., Disruption Of Sertoli-germ Cell Adhesion Function In The Seminiferous Epithelium Of The Rat Testis Can Be Limited To Adherens Junctions Without Affecting The Blood-testis Barrier Integrity: An In Vivo Study Using An Androgen Suppression Model. , Journal of Cellular Physiology. 2005, 205: 141-157. |
| Researcher : Cheng THT |
| List of Research Outputs |
| Cheng T.H.T., Cheung H.K., Wang L., Wang T. and Xu R.J., Effects of green tea extracts on learning and memory behaviour in rats, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S89. |
| Researcher : Cheung HK |
| List of Research Outputs |
| Cheng T.H.T., Cheung H.K., Wang L., Wang T. and Xu R.J., Effects of green tea extracts on learning and memory behaviour in rats, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S89. |
| Researcher : Cheung WT |
| List of Research Outputs |
| Cheung W.T., Au S.C.L., Cheung A.N.Y., Ngan H.Y.S. and Wong A.S.T., Pigment Epithelium-Derived Factor, a Gene Down-Regulated by Estrogen, Mediates Growth Inhibition in Human Ovarian Surface Epithelial and Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-32). 2006. |
| Cheung W.T., Au S.C., Cheung A.N.Y., Ngan H.Y.S., Tombran-Tink J., Auersperg N. and Wong A.S.T., Pigment epithelium-derived factor is estrogen sensitive and inhibits the growth of human ovarian cancer and ovarian surface epithelial cells, Endocrinology. 2006, 147(9): 4179-91. |
| Researcher : Cheung WT |
| List of Research Outputs |
| Cheung W.T., Au S.C.L., Cheung A.N.Y., Ngan H.Y.S. and Wong A.S.T., Pigment Epithelium-Derived Factor, a Gene Down-Regulated by Estrogen, Mediates Growth Inhibition in Human Ovarian Surface Epithelial and Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-32). 2006. |
| Cheung W.T., Au S.C., Cheung A.N.Y., Ngan H.Y.S., Tombran-Tink J., Auersperg N. and Wong A.S.T., Pigment epithelium-derived factor is estrogen sensitive and inhibits the growth of human ovarian cancer and ovarian surface epithelial cells, Endocrinology. 2006, 147(9): 4179-91. |
| Researcher : Chow BKC |
| Project Title: | Secretin as a neuropeptide |
| Investigator(s): | Chow BKC, Yung WH |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2002 |
| Completion Date: | 08/2005 |
| Abstract: |
| The project attempts to:- 1) study the distributions fo secretin and secretin receptor in the rat brain by in situ hybridization and immunocytochemical staining; 2) investigate the electrophysiological functions of secretin in regulating Purkinje cell functions, particularly on neuronal pathways and mechanisms; and 3) study the potential electrophysiological activities of secretin in discrete cells from other brain regions. |
| Project Title: | Regulation of the human secretin receptor gene |
| Investigator(s): | Chow BKC |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To investigate the interactions of GC motifs within the core promoter region of the hSR gene with Sp1 and Sp3. In addition, the connections between the Sp proteins, cytosine methylation and methylation imprinting will also be studied to elucidate the epigenetic regulation of expression in the hSR gene. |
| Project Title: | Mapping of human secretin and its receptor gene expression in human cerebellum |
| Investigator(s): | Chow BKC |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To demonstrate the presence of their transcripts in human cerebellum using real-time quantitative PCR and in situ hybridization, and the localization of secretin peptide by immunohistochemical staining and confocal microscopy. |
| Project Title: | A conditional knockout animal model for secretin |
| Investigator(s): | Chow BKC |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Abstract: |
| N/A |
| Project Title: | A conditional knockout animal model for secretin |
| Investigator(s): | Chow BKC, Sham MH, Yung WH |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 01/2005 |
| Abstract: |
| To develop an embryonic stem cell-line carrying the floxed secretin gene flanked by loxP sites; to generate secretin null mutant to investigate the physiology of a general secretin KO; to investigate the physiological effects of neuron-specific and Purkinje-specific secretin gene KO. |
| Project Title: | Molecular Evolution of Growth Hormone Releasing Hormone and Its Receptor in non-mammalian species |
| Investigator(s): | Chow BKC |
| Department: | Zoology |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2006 |
| Abstract: |
| 1) Clone and functionally characterize GHRH and its receptor from representative species of fish (zebrafish and/or goldfish), frog (Xenopus) and bird (chicken). 2) Phylogenetic analyses to show the novel evolutionary scheme of GHRH and its receptor in vertebrates. 3) Provide evidence to show that previously identified GHRHs are in fact only related peptides. Background: In mammals, GHRH is released from the arcuate nucleus via the axonal fibers to the hypophysial portal vessels, and is subsequently delivered to the anterior pituitary where it triggers synthesis and pulsatile release of GH through the GHRH receptor (GHRHR). In 1982, GHRH was isolated from human pancreatic islet tumors that caused acromegaly (4-5). A number of GHRH-like peptides have been characterized from various species, but, as shown in our phylogenetic study (Fig 1), they are more similar to mammalian PACAP-related peptides (PRP) rather than mammalian GHRHs. Moreover, the distributions of these GHRH-like peptides in brain are different when compared to mammalian GHRHs. Functionally, carp GHRH-like peptide cannot stimulate GH release in goldfish and carp pituitary cells. These clearly indicated to us that the "real" GHRHs have indeed never been characterized in non-mammalian species. Several non-mammalian vertebrate genomic databases were completely or partially released including avian [Gallus Gallus (Chicken)], amphibian [Xenopus T. (Africa clawed frog)) and fish [Danio rerio (Zebrafish); Takifugu rubripes (Fugu)]. In our bio-informatic analyses, we have identified genes that shared high homology with mammalian GHRHs (Figure 2) and their receptors (Figure 3). We propose here to characterize these genes and test their functions. Information obtained should be crucial to mankind as bird and fish are the most important protein sources in human diet, and this project is therefore potentially significant to raise food productivity in related agricultural species. |
| Project Title: | Secretin: a putative neurosecretory hormone that regulates water homeostasis in the hypothalamus-pituitary-adrenal axis |
| Investigator(s): | Chow BKC |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2006 |
| Abstract: |
| N/A |
| Project Title: | Secretin: a putative neurosecretory hormone that regulates water homeostasis in the hypothalamus-pituitary-adrenal axis |
| Investigator(s): | Chow BKC, Chan YS, Chung SK |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 01/2006 |
| Abstract: |
| To identity the functions of secretin in the hypothalamus and pituitary with a special focus on its potential modulatory role in vasopressin (Vp) synthesis and release; to substantiate the hypothesis that secretin is a neurosecretory hormone released from the posterior pituitary into the systemic circulation; to investigate the function of secretin as an antidiuretic hormone to regulate water reabsorption in the kidney. |
| List of Research Outputs |
| Chen K., Wang J.J., Yung W.H., Chan Y.S. and Chow B.K.C., Excitatory effect of histamine on neuronal activity of rat globus pallidus by activation of H2 receptors in vitro, Neuroscience Research . 2005, 53: 288-297. |
| Chow B.K.C., Chu Y.S.J. and Chung C.K.S., A putative role of secretin to regulate water homeostasis, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Chow B.K.C., Outstanding Researcher Award, HKU, 2005, 2005. |
| Chow B.K.C., Plenary lecture and Scientific Advisors Committee, 7th International Symposium on VIP, PACAP and related Peptides, Rouen, France. 2005. |
| Chu J.Y.S., Lee T.O., Chung S.C.K., Lai C.H., Chan Y.S., Yung W.H. and Chow B.K.C., Secretin in the hypothalamo-pituitary-renal system: Implication of secretin in regulating water homeostasis, Proceedings of the 88th Annual Meeting of the Endocrinology Society. 2006, OR48-6. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Actions of secretin on the hypothalamic magnocellular neurons: Implications for the control of body water homeostasis, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Actions of secretin on the hypothalamic magnocellular neurons: Implications for the control of body water homostasis, Regulatory Peptides. 2006, 130(3): 159. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Endogenous release of secretin from the hypothalamus, Annals New York Academy of Sciences. 2006, 1070: p.196-200. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Secretin: a pleiotrophic hormone , Annals New York Academy of Sciences. 2006, 1070: p27-50. |
| Chu Y.S.J., Lee T.O., Siu K.Y. and Chow B.K.C., The Secretin/Pituitary Adenylate Cyclase-Activating Polypeptide/Vasoactive Intestinal Polypeptide Superfamily in the Central Nervous System, Central Nervous System Agents in Medicinal Chemistry. 2006, 6: 27-57. |
| Guillemot J., Anouar Y., Montero-Hadjadje M., Grouzmann E., Grumolato L., Roshmaninho-Salgado J., Turquier V., Duparc C., Lefebvre H., Plouin P.F., Klein M., Muresan M., Chow B.K.C., Vaudry H. and Yon L., Circulating EM66 is a highly sensitive marker for the diagnosis and follow-up of pheochromocytoma, Int. J. Cancer. 2006, 118: 2003-2012. |
| Kwok Y.Y., Chu Y.S.J., Vaudry H., Yon L., Anouar Y. and Chow B.K.C., Cloning and characterization of a PACI receptor hop-1 splice variant in goldfish (Carassius auratus), General and Comparative Endocrinology. 2006, 145: 188-196. |
| Lam P.Y., Shannon G., Chow B.K.C., Lee T.O., Julie V. and Lee V.H.Y., Alpini Gianfranco 2006 Cholic acid stimulates bile duct proliferation both in vivo and in vitro via regulation of the expression and secretion of secretin by cholangiocytes: Novel evidence for autocrine/paracrine regulation of cholangiocyte proliferation by secretin, American Association for the study of Liver Diseases, USA. 2006. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), Regulatory Peptides. 2006, 130(3): 167. |
| Lam P.Y., Lee T.O., Choi H.S. and Chow B.K.C., Localization of small heterodimer partner (SHP) with the secretin in mouse duodenal cells, Annals New York Academy of Sciences. 2006, 1070: 371-375. |
| Lee T.O., Lee H.Y.V., Yuan Y. and Chow B.K.C., Identification of repressor element 1 in secretin/PACAP/VIP genes, Annals New York Academy of Sciences. 2006, 1070: 388-392. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1, Annals New York Academy of Sciences. 2006, 1070: 393-398. |
| Lee T.O., Lee H.Y.V., Lee P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lee T.O., Lee H.Y.V., Yuen P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, Regulatory Peptides. 2006, 130(3): 167-168. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, Regulatory Peptides. 2006, 130(3): 167. |
| Liu S.Y.K., Tsang J.S.H., Leung P.C., Chow B.K.C. and Wan J.M.F., Proteomics of Macrophage; an Approach to Understand Lipopolysaccharide-challenged inflammation, Molecular & Cellular Proteomics 4.8:S231, HUPO 4th Annual World Congress, August 28-September 1, 2005, Munich. 2005. |
| Man C.W.Y., Rosa J., Lee T.O., Lee H.Y.V., Chow B.K.C., Lo K.W., Doxsey S., Wu Z.G., Kwong Y.L., Jin D., Cheung A. and Tsao G.S.W., Latent membrane protein 1 suppresses RASSFIA expression, disrupts microtubule structures and induces chromosomal aberrations in human epithelial cells, Oncogene. 2006, 1-12. |
| Ng S.M., Chow B.K.C. and Wong V.C.N., The human secretin gene in children with autistic spectrum disorder: screening for polymorphisms and mutations., Journal of Child Neurology. 2005, 20(8): 701-704. |
| Shannon G., Lee H.Y.V., Lam P.Y. and Chow B.K.C., Alpini Gianfranco 2006 Secretin inhibits cholangiocarcinoma growth via inhibition of ERK1/2 and p38-dependent signaling, American Association for the study of Liver Diseases, U.S.A.. 2006. |
| Siu K.Y., Sham M.H. and Chow B.K.C., Secretin is widely expressed during mouse embryonic development, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Siu K.Y., Sham M.H. and Chow B.K.C., Secretin is widely expressed during mouse embryonic development, Regulatory Peptides. 2006, 130(3): 176. |
| Siu K.Y., Sham M.H. and Chow B.K.C., The prenatal expression of secretin receptor, Annals New York Academy of Sciences. 2006, 1070: p561-565. |
| Yeung C.M., An B.S., Cheng C.K., Chow B.K.C. and Leung C.K., Expression and transcriptional regulation of the GnRH receptor gene in human neuronal cells., Mol. Hum. Reprod.. 2005, 11(11): 837-842. |
| Yung W.H., Chan Y.S., Chow B.K.C. and Wang J.J., The role of secretin in the cerebellum, The Cerebellum. 2006, 5: 43-48. |
| Zhu J.N., Yung W.H., Chow B.K.C., Chan Y.S. and Wang J.J., The cerebellar-hypothalamic circuits: Potential pathways underlying cerebellar involvement in somatic-visceral integration, Brain Research Reviews. 2006, 52: 93-106. |
| Researcher : Chow YCK |
| List of Research Outputs |
| Chow Y.C.K., Yeung Y.S., Hon C.C., Zeng F., Law K.M. and Leung F.C.C., Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells, Federation of European Biochemical Society letters. 2005, 579: 6699-6704. |
| Researcher : Chu JYS |
| List of Research Outputs |
| Chu J.Y.S., Lee T.O., Chung S.C.K., Lai C.H., Chan Y.S., Yung W.H. and Chow B.K.C., Secretin in the hypothalamo-pituitary-renal system: Implication of secretin in regulating water homeostasis, Proceedings of the 88th Annual Meeting of the Endocrinology Society. 2006, OR48-6. |
| Researcher : Chu YSJ |
| List of Research Outputs |
| Chow B.K.C., Chu Y.S.J. and Chung C.K.S., A putative role of secretin to regulate water homeostasis, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Actions of secretin on the hypothalamic magnocellular neurons: Implications for the control of body water homeostasis, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Actions of secretin on the hypothalamic magnocellular neurons: Implications for the control of body water homostasis, Regulatory Peptides. 2006, 130(3): 159. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Endogenous release of secretin from the hypothalamus, Annals New York Academy of Sciences. 2006, 1070: p.196-200. |
| Chu Y.S.J., Yung W.H. and Chow B.K.C., Secretin: a pleiotrophic hormone , Annals New York Academy of Sciences. 2006, 1070: p27-50. |
| Chu Y.S.J., Lee T.O., Siu K.Y. and Chow B.K.C., The Secretin/Pituitary Adenylate Cyclase-Activating Polypeptide/Vasoactive Intestinal Polypeptide Superfamily in the Central Nervous System, Central Nervous System Agents in Medicinal Chemistry. 2006, 6: 27-57. |
| Kwok Y.Y., Chu Y.S.J., Vaudry H., Yon L., Anouar Y. and Chow B.K.C., Cloning and characterization of a PACI receptor hop-1 splice variant in goldfish (Carassius auratus), General and Comparative Endocrinology. 2006, 145: 188-196. |
| Researcher : Chung CKS |
| List of Research Outputs |
| Chow B.K.C., Chu Y.S.J. and Chung C.K.S., A putative role of secretin to regulate water homeostasis, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Researcher : Chung SCK |
| List of Research Outputs |
| Chu J.Y.S., Lee T.O., Chung S.C.K., Lai C.H., Chan Y.S., Yung W.H. and Chow B.K.C., Secretin in the hypothalamo-pituitary-renal system: Implication of secretin in regulating water homeostasis, Proceedings of the 88th Annual Meeting of the Endocrinology Society. 2006, OR48-6. |
| Researcher : Guo X |
| List of Research Outputs |
| Guo X., Philipsen S. and Tan-Un K.C., Characterization of human cytoglobin gene promoter region, Biochimica et Biophysica Acta. 2006, 1759: 208-215. |
| Researcher : Hon CC |
| List of Research Outputs |
| Chow Y.C.K., Yeung Y.S., Hon C.C., Zeng F., Law K.M. and Leung F.C.C., Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells, Federation of European Biochemical Society letters. 2005, 579: 6699-6704. |
| Hon C.C., Lee Y.F., Ching W.K., Ng K.P., Ng Y.W. and Leung F.C.C., Molecular Dating of the Epidemiological Events in Relation to the Emergence of Very Virulent Infectious Bursal Disease Virus, Poultry Science 84(Supp). 2005, 73. |
| Hon C.C., Lam T.Y., Drummond A., Rambaut A., Lee Y.F., Yip C.W., Zeng F., Lam P.Y., Ng P.T.W. and Leung F.C.C., Phylogenetic analysis reveals coincidence of the expansion of very virulent Infectious Bursal Disease Virus and the reassortment of its genome segment B, Journal of Virology. 2006, 80: 8503-8509. |
| Li J., Wang Y., Hon C.C. and Leung F.C.C., Cloning of Smad 1, Smad5 and Smad8 from the Chicken Ovary and Characterization of Their Expression during Chicken Ovarian Development, Poultry Science 84(Supp). 2005, 74. |
| Wong T.Y., Hon C.C. and Leung F.C.C., Molecular characterization of IBDV-induced in vitro apoptosis using cDNA microarrays, Proceedings of the Third Symposium on Genetics of Animal of Health, Ames, IA. 2005, p.130. |
| Researcher : Hoo RLC |
| Project Title: | Role of adiponectin in mediating the therapeutic actions of the anti-diabetic drug thiazolidinediones |
| Investigator(s): | Hoo RLC, Xu A, Lam KSL |
| Department: | Medicine |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2005 |
| Abstract: |
| Thiazolidinediones (TZDs), such as rosiglitazone and pioglitazone, are peroxisome-proliferator-activated receptor gamma (PPAR-gamma) agonists widely used in the treatment of type 2 diabetes. The cellular mechanism of action of TZDs involves binding to the PPAR-gamma, thus altering the expression of PPAR-gamma responsive gene in fat cells, as well as other cell types such as endothelial cells, macrophage, monocytes, vascular smooth cells (1,2). We and others have shown that they can reduce insulin resistance, hyperglycaemia, and endothelial dysfunction, an early change in atherosclerosis (3-6). However, the mechanism underlying the ability of PPAR-gamma agonists to improve in vivo hyperglycemia and insulin resistance is not well understood. Adipose tissue is now recognized as an important endocrine organ as it can secrete a variety of biologically active peptides such as adiponectin, plasminogen activated inhibitor I (PAI-1), resistin, leptin, TNF-alpha and IL-6. These adipokines are suggested to be critically involved in regulating systemic energy metabolism, insulin sensitivity, cardiovascular tone and immune response (6,7). Our group has been actively studying the regulation and function of adiponectin in recent years. We have shown that treatment with adiponectin can result in direct beneficial effects on insulin resistance, hyperglycaemia, dyslipidaemia and atherogenesis. Many pharmacological studies showed that TZDs, including rosiglitazone, can elevate the mRNA expression and plasma levels of adiponectin in animals and in patients with type 2 diabetes (10,11). However, whether adiponectin mediates the TZD's metabolic effects in T2DM and its related metabolic syndrome remains uncertain. Therefore, in the present study, we seek to investigate the mechanism of metabolic action of a potent TZD, rosiglitazone, in the presence or absence of adiponectin, by using a genetically modified mouse model that lacks both the adiponectin gene and leptin receptor. Major Objectives: The major aim of this study is to elucidate the involvement of adiponectin in the therapeutic function of the potent TZD, rosigltiazone, in the treatment of T2DM. The specific objectives are: 1. To knockout the adiponectin gene from the db/db diabetic mouse model. 2. To use the double knockout mice generated in Objective 1 to investigate whether adiponectin is required for the anti-diabetic action of rosiglitazone. |
| List of Research Outputs |
| Researcher : Hu K |
| List of Research Outputs |
| Hu K. and Leung P.C., Complete, precise, and innocuous loss of multiple introns in the currently intronless, active cathepsin L-like genes, and inference from this event, Molecular Phylogenetics and Evolution. 2006, 38: 685-696. |
| Researcher : Hui JHL |
| List of Research Outputs |
| Tiu H.K., Hui J.H.L., He J.G., Tobe S.S. and Chan S.M., Characterization of Vitellogenin in the Shrimp Metapenaeus ensis: Expression Studies and Hormonal Regulation of MeVg1 Transcription In Vitro, Molecular Reproduction and Development. 2006, 73: 424-436. |
| Tiu H.K., Hui J.H.L., Mak A.S.C., He J.G. and Chan S.M., Equal contribution of hepatopancreas and ovary to the production of vitellogenin (PmVg1) transcripts in the tiger shrimp, Penaeus monodon, Aquaculture. 2006, 254: 666-674. |
| Researcher : Hui KH |
| List of Research Outputs |
| Chen J., Zhao M., Hui K.H. and Leung F.C.C., Molecular Characterization of Foot-and-mouth Disease Virus in Hong Kong During 2001-2002, Virus Genes. 2006, 32: 139-143. |
| Researcher : Hui PY |
| List of Research Outputs |
| Wan J.M.F., Hui P.Y. and Sit W.H., Induction of S phase cell arrest and caspase activation by polysaccharide peptide (PSP) isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cells, 10th World Congress on Advances in Oncology and 8th International Symposium on Molecular Medicine, 2005 Greece. 2005. |
| Researcher : Huo L |
| List of Research Outputs |
| Wong A.O.L., Li W., Leung C.Y., Huo L. and Zhou H., Pituitary adenylate cyclase-activating polypeptide (PACAP) as a growth hormone (GH)-releasing factor in grass carp: I. Functional coupling of cAMP- and Ca2+/ calmodulin-dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells. , Endocrinology. USA, The Endocrine Society, 2005, 146: 5407-5424. |
| Researcher : Jiang P |
| List of Research Outputs |
| Ngai H.Y.H., Sit W.H., Jiang P., Thongboonkerd V. and Wan J.M.F., Comparative Proteomics Analysis Reveals Haptoglobin as a Possible Novel Marker in Human Membranous Nephropathy, Molecular ＆ Cellular Proteomics, Human Proteome Organisation, 5th Annual World Congress, October 28 - November 1, 2006, Long Beach, California. 2006, 5.10: 496. |
| Researcher : Jiang Y |
| List of Research Outputs |
| Wong A.O.L., Zhou H., Jiang Y. and Ko K.W., Feedback regulation of growth hormone synthesis and secretion in fish and emerging concept of intrapituitary feedback loop, Comparative Biochemistry of Physiology . 2006, (Part A) 144: 284-305. |
| Researcher : Ko KW |
| List of Research Outputs |
| Wong A.O.L., Zhou H., Jiang Y. and Ko K.W., Feedback regulation of growth hormone synthesis and secretion in fish and emerging concept of intrapituitary feedback loop, Comparative Biochemistry of Physiology . 2006, (Part A) 144: 284-305. |
| Wong A.O.L., Zhou H., Ko K.W. and Ho K.K., 在草魚中新發現的生長激素合成和分泌的反饙調控機理, 中國科技發展精典文庫,第一部分 農業科技, 第七章,漁業科技, 2006, pp.1-7. |
| Researcher : Ko RCC |
| Project Title: | Prevalence of two protozoan pathogens, Girardia and Cryptosporidium, in the water supplies of Hong Kong |
| Investigator(s): | Ko RCC, Mak C, Wu R.S.S., Singh M. |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 10/2002 |
| Completion Date: | 09/2005 |
| Abstract: |
| The study attempts to:- 1) determine the general prevalence of two organisms, Giardia intestinalis and Cryptosporidium parvum, in Hong Kong water supplies; 2) determine the major source of contamination; 3) determine, if any, the seasonal incidence of these two organisms in various water supplies. |
| Project Title: | Modulation of cell-mediated immune and inflammatory responses by Trichinella pseudospiralis during the acute muscle phase of infection- the roles of adhesion molecules and chemokines |
| Investigator(s): | Ko RCC, Mak C |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2002 |
| Abstract: |
| To further characterise the cell-mediated immune (systemic) response during the early phase of worm infection; to determine the roles of adhesion molecules and chemokines in suppression of inflammatory/immune response after the newborn larvae have invaded the striated muscles; to test the hypothesis that this species of nematode produce effector molecules to evade host's response. |
| Project Title: | Study on the structure of TCTP gene of Trichinella pseudospiralis (Nematoda) |
| Investigator(s): | Ko RCC, Mak |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 07/2005 |
| Abstract: |
| The present study represents a follow-up work on our previous efforts to clone and characterize the heat-induced translationally controlled tumour protein (TCTP) of a tissue dwelling nematode, Trichinella pseudopsiralis. We had succeeded to clone both the full-length and N-terminal cDNA sequences of TCTP. The full-length gene comprises 534 bp, with an open reading frame encoding 177 amino acid residues and conserved regions. The recombinant protein( 23 kDA) was also expressed. Antibodies were produced against the protein. However, more studies are required to complete the work on gene characterization and evaluation of the recombinant protein before a paper can be published. TCTP was originally found in a human tumour in 1980's. Later, it has been found in many organisms including some parasitic protozoans and helminths. It is known to play an important role in cell-cycle progression and malignant transformation of cells. The protein can also act as a histamine-release factor and has an anti-apoptotic activity. However, the extent of its functions is not known. In parasitic organisms the protein is suspected to play a key role in host-parasite interactions and in survival. The results of the present project may provide the basis for further studies on the functions of this protein. The objectives are: (1) To complete the determination of the gene structure of TCTP.(2) To elucide its genomic organization. (3). To determine its 5' translational site. (4) To confirm the size of the recombinant protein. |
| List of Research Outputs |
| Ko R.C.C., Fish-bone parasitic zoonoses, In: Woo, P.T.K. editor, Fish Diseases and Disorder Vol. I, Protozoan and Metazoan Infections, 2nd Edition, CAB International. 2006, p.592-628. |
| Lee K.M. and Ko R.C.C., Cell-mediated response at the muscle phase of Trichinella pseudospiralis and Trichinella spiralis infections, Parasitology Research. 2006, 99: 70-77. |
| Wu W.K., Mak C. and Ko R.C.C., Cloning and characterization of the Cu/Zn superoxide dismutase of Trichinella pseudospiralis, Parasitology Research. 2005, 98: 281-287. |
| Researcher : Kwan WY |
| List of Research Outputs |
| Wong A.S.T., Lee A.S., Wong K.Y., Kwan W.Y. and Wong E.S.Y., Deregulation of the Stress-Inducible GRP78 in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.732 (Abstract No. P3-377). 2006. |
| Researcher : Kwok YY |
| List of Research Outputs |
| Kwok Y.Y., Chu Y.S.J., Vaudry H., Yon L., Anouar Y. and Chow B.K.C., Cloning and characterization of a PACI receptor hop-1 splice variant in goldfish (Carassius auratus), General and Comparative Endocrinology. 2006, 145: 188-196. |
| Researcher : Lam PY |
| List of Research Outputs |
| Hon C.C., Lam T.Y., Drummond A., Rambaut A., Lee Y.F., Yip C.W., Zeng F., Lam P.Y., Ng P.T.W. and Leung F.C.C., Phylogenetic analysis reveals coincidence of the expansion of very virulent Infectious Bursal Disease Virus and the reassortment of its genome segment B, Journal of Virology. 2006, 80: 8503-8509. |
| Lam P.Y., Shannon G., Chow B.K.C., Lee T.O., Julie V. and Lee V.H.Y., Alpini Gianfranco 2006 Cholic acid stimulates bile duct proliferation both in vivo and in vitro via regulation of the expression and secretion of secretin by cholangiocytes: Novel evidence for autocrine/paracrine regulation of cholangiocyte proliferation by secretin, American Association for the study of Liver Diseases, USA. 2006. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), Regulatory Peptides. 2006, 130(3): 167. |
| Lam P.Y., Lee T.O., Choi H.S. and Chow B.K.C., Localization of small heterodimer partner (SHP) with the secretin in mouse duodenal cells, Annals New York Academy of Sciences. 2006, 1070: 371-375. |
| Shannon G., Lee H.Y.V., Lam P.Y. and Chow B.K.C., Alpini Gianfranco 2006 Secretin inhibits cholangiocarcinoma growth via inhibition of ERK1/2 and p38-dependent signaling, American Association for the study of Liver Diseases, U.S.A.. 2006. |
| Researcher : Lam TY |
| List of Research Outputs |
| Hon C.C., Lam T.Y., Drummond A., Rambaut A., Lee Y.F., Yip C.W., Zeng F., Lam P.Y., Ng P.T.W. and Leung F.C.C., Phylogenetic analysis reveals coincidence of the expansion of very virulent Infectious Bursal Disease Virus and the reassortment of its genome segment B, Journal of Virology. 2006, 80: 8503-8509. |
| Lam T.Y. and Leung F.C.C., Original of PRRS virus: Towards a better understanding beyond the epidemiology, Proceedings of the 2005 International PRRS Symposium, Dec 2-3, 2005, St. Louis, Missouri. 2005, p.63. |
| Researcher : Law KM |
| List of Research Outputs |
| Chow Y.C.K., Yeung Y.S., Hon C.C., Zeng F., Law K.M. and Leung F.C.C., Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells, Federation of European Biochemical Society letters. 2005, 579: 6699-6704. |
| Researcher : Lee CL |
| List of Research Outputs |
| Lee C.L., Sit W.H. and Wan J.M.F., Cyclosporine A-like Immunomodulatory Benefits of Polysaccharopeptide (PSP) Derived from Coriolus Versicolor in Activated Human T-cells, Inflammation research 54(supp. 2):S130, 7th World Congress of Inflammation, Australia. 2005. |
| Lee C.L., Yang X. and Wan J.M.F., The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor, Enzyme and Microbial Technology. 2006, 38: 14-21. |
| Wan J.M.F., Sit W.H., Lee C.L., Fu H.M.K. and Chan D.K.O., Protection of lethal toxicity of endotoxin by Salvia miltiorrhiza BUNGE is via reduction in tumor necrosis factor alpha release and liver injury, International Immunopharmacology. 2006, 6: 750-758. |
| Researcher : Lee HYV |
| List of Research Outputs |
| Lee T.O., Lee H.Y.V., Yuan Y. and Chow B.K.C., Identification of repressor element 1 in secretin/PACAP/VIP genes, Annals New York Academy of Sciences. 2006, 1070: 388-392. |
| Lee T.O., Lee H.Y.V., Lee P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lee T.O., Lee H.Y.V., Yuen P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, Regulatory Peptides. 2006, 130(3): 167-168. |
| Man C.W.Y., Rosa J., Lee T.O., Lee H.Y.V., Chow B.K.C., Lo K.W., Doxsey S., Wu Z.G., Kwong Y.L., Jin D., Cheung A. and Tsao G.S.W., Latent membrane protein 1 suppresses RASSFIA expression, disrupts microtubule structures and induces chromosomal aberrations in human epithelial cells, Oncogene. 2006, 1-12. |
| Shannon G., Lee H.Y.V., Lam P.Y. and Chow B.K.C., Alpini Gianfranco 2006 Secretin inhibits cholangiocarcinoma growth via inhibition of ERK1/2 and p38-dependent signaling, American Association for the study of Liver Diseases, U.S.A.. 2006. |
| Researcher : Lee KM |
| List of Research Outputs |
| Lee K.M. and Ko R.C.C., Cell-mediated response at the muscle phase of Trichinella pseudospiralis and Trichinella spiralis infections, Parasitology Research. 2006, 99: 70-77. |
| Researcher : Lee KY |
| List of Research Outputs |
| Wong A.O.L., Chuk M.C.Y., Chan H.C. and Lee K.Y., Mechanisms for gonadotropin-releasing hormone potentiation of growth hormone rebound following norepinephrine inhibition in goldfish pituitary cells, American Journal of Physiology. 2006. |
| Researcher : Lee PY |
| Project Title: | Tight-junction claudin-19 in kidney development and renal disease |
| Investigator(s): | Lee PY, Luk JMC, Tam PC |
| Department: | Surgery |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To isolate, identify and characterize a novel tight-junction molecule, claudin 19, which was initially found highly expressed in kidney. |
| Project Title: | Molecular analysis of a novel signaling molecule VAD1.2 highly conseved in mammalian germ cells |
| Investigator(s): | Lee PY, Luk Dr |
| Department: | Surgery |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2005 |
| Abstract: |
| Germ cells are "stem-like" cell types capable of self-renewal and differentiation into highly specialized fertilizing cell types, resulting in the formation of spermatozoa during spermatogenesis (Cheng and Mruk, 2002). Haploid germ cells are initially resided on the basal lamina of the seminiferous epithelium in the testes and they migrate towards the lumens after passing through the tight junctions for further developments and maturations (Cheng and Mruk, 2002). In this process, the cell-cell associations and dissociations, the restructurings of the junctions and their linked underlying cytoskeletons, and the regulations of gene expressions are noted. As such, different gene expression profiles can be observed at different stages of germ cell development, making it interesting to study the arrays of gene expressions. However, it will be techically difficult and tedious to isolate germ cells originated in different stages. For instance, one can manually isolate different spermatogenic stages based on their distinct transillumination properties under stereomicroscope (Chen et al, 2003; Parvinen and Vanha-Perttula, 1972). In order to simplify the procedure for the study of gene regulations in different germ cell types in the testis, vitamin A-deficiency (VAD) and post-vitamin A replenishment (PVA) rat model and differential display reverse transcription-polymerase chain reaction (RT-PCR) are used to isolate and identify stage- or cell type-specific genes in the testis (Luk et al, 2003). In this model, adult rats are first fed with vitamin A-deficient diets, which leads to the spermatogenesis arrest and subsequent germ cell depletion from the epithelium (Luk et al, 2003; Lee et al, 2004). At that time, spermatogonia and preleptotene spermatocytes are the only two germ cell types found in the seminiferous tubules (Luk et al, 2003; Lee et al, 2004). Spermatogenesis is resumed in these rats by treating them with retinol, which can stimulate the proliferative activity of spermatogonia and their development to advanced germ cell types, resulting in the synchronization of the spermatogenic stages (van Pelt and de Rooij, 1990, 1991). Testes were isolated from different post-treatment days and subjected for differential display (Luk et al, 2003). As such, a number of differentially expressed genes, such as VAD 1.2, VAD 1.3, VAD 3.4, and VAD 28, were identified (Luk et al, 2003). Among those genes, VAD 1.2 was selected for further characterization, since it was found largely in advanced germ cell types (Luk et al, 2003). Advanced germ cells take part in several important cellular processes, such as spermiogenesis and spermiation, making them as one of the most essential cell types in coordinating these processes for turning germ cells into mature spermatozoa. The production of a mature and functional spermatozoan will be a critical step for successful fertilization. As such, the understanding of genes associated with these advanced germ cells and their functions are one of the important preliminary parameters for further understanding infertility and for the development of the its diagnosis and treatment. References:Chen YM, Lee NP, Mruk DD, Lee WM, Cheng CY (2003) Fer kinase/FerT and adherens junction dynamics in the testis: an in vitro and in vivo study. Biol Reprod 69: 656-672Cheng CY, Mruk DD (2002) Cell junction dynamics in the testis: Sertoli-germ cell interactions and male contraceptive development. Physiol Rev 82: 825-874 Lee KF, Yeung WS, Chow JF, Shum CK, Luk JM (2004) Different testicular gene expression patterns in the first spermatogenic cycle of postnatal and vitamin A-deficient rat testis. Biol Reprod 70: 1010-1017Luk JM, Mok BW, Shum CK, Yeung WS, Tam PC, Tse JY, Chow JF, Woo J, Kam K, Lee KF (2003) Identification of novel genes expressed during spermatogenesis in stage-synchronized rat testes by differential display. Biochem Biophys Res Commun 307: 782-790Parvinen M, Vanha-Perttula T (1972) Identification and enzyme quantitation of the stages of the seminiferous epithelial wave in the rat. Anat Rec 174: 435-449van Pelt AM, de Rooij DG (1990) Synchronization of the seminiferous epithelium after vitamin A replacement in vitamin A-deficient mice. Biol Reprod 43: 363-367van Pelt AM, de Rooij DG (1991) Retinoic acid is able to reinitiate spermatogenesis in vitamin A-deficient rats and high replicate doses support the full development of spermatogenic cells. Endocrinology 128: 697-704 |
| List of Research Outputs |
| Lee T.O., Lee H.Y.V., Lee P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Researcher : Lee PY |
| List of Research Outputs |
| Lee T.O., Lee H.Y.V., Lee P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Researcher : Lee TO |
| Project Title: | Transcriptional regulation of a nasopharyngeal carcinoma tumor suppressor: RASSF1A |
| Investigator(s): | Lee TO, Chow BKC |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To elucidate the basal transcription regulation mechanisms of RASSFIA in normal - RASSF1A-expressing and RASSF1A-non-expressing NPC cells; to fine map the core promoter region of RASSF1 gene; identify transcription factor(s) that regulates RASSF1A expression. |
| List of Research Outputs |
| Chu J.Y.S., Lee T.O., Chung S.C.K., Lai C.H., Chan Y.S., Yung W.H. and Chow B.K.C., Secretin in the hypothalamo-pituitary-renal system: Implication of secretin in regulating water homeostasis, Proceedings of the 88th Annual Meeting of the Endocrinology Society. 2006, OR48-6. |
| Chu Y.S.J., Lee T.O., Siu K.Y. and Chow B.K.C., The Secretin/Pituitary Adenylate Cyclase-Activating Polypeptide/Vasoactive Intestinal Polypeptide Superfamily in the Central Nervous System, Central Nervous System Agents in Medicinal Chemistry. 2006, 6: 27-57. |
| Lam P.Y., Shannon G., Chow B.K.C., Lee T.O., Julie V. and Lee V.H.Y., Alpini Gianfranco 2006 Cholic acid stimulates bile duct proliferation both in vivo and in vitro via regulation of the expression and secretion of secretin by cholangiocytes: Novel evidence for autocrine/paracrine regulation of cholangiocyte proliferation by secretin, American Association for the study of Liver Diseases, USA. 2006. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), Regulatory Peptides. 2006, 130(3): 167. |
| Lam P.Y., Lee T.O., Choi H.S. and Chow B.K.C., Localization of small heterodimer partner (SHP) with the secretin in mouse duodenal cells, Annals New York Academy of Sciences. 2006, 1070: 371-375. |
| Lee T.O., Lee H.Y.V., Yuan Y. and Chow B.K.C., Identification of repressor element 1 in secretin/PACAP/VIP genes, Annals New York Academy of Sciences. 2006, 1070: 388-392. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1, Annals New York Academy of Sciences. 2006, 1070: 393-398. |
| Lee T.O., Lee H.Y.V., Lee P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lee T.O., Lee H.Y.V., Yuen P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, Regulatory Peptides. 2006, 130(3): 167-168. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, Regulatory Peptides. 2006, 130(3): 167. |
| Man C.W.Y., Rosa J., Lee T.O., Lee H.Y.V., Chow B.K.C., Lo K.W., Doxsey S., Wu Z.G., Kwong Y.L., Jin D., Cheung A. and Tsao G.S.W., Latent membrane protein 1 suppresses RASSFIA expression, disrupts microtubule structures and induces chromosomal aberrations in human epithelial cells, Oncogene. 2006, 1-12. |
| Researcher : Lee TO |
| List of Research Outputs |
| Chu J.Y.S., Lee T.O., Chung S.C.K., Lai C.H., Chan Y.S., Yung W.H. and Chow B.K.C., Secretin in the hypothalamo-pituitary-renal system: Implication of secretin in regulating water homeostasis, Proceedings of the 88th Annual Meeting of the Endocrinology Society. 2006, OR48-6. |
| Chu Y.S.J., Lee T.O., Siu K.Y. and Chow B.K.C., The Secretin/Pituitary Adenylate Cyclase-Activating Polypeptide/Vasoactive Intestinal Polypeptide Superfamily in the Central Nervous System, Central Nervous System Agents in Medicinal Chemistry. 2006, 6: 27-57. |
| Lam P.Y., Shannon G., Chow B.K.C., Lee T.O., Julie V. and Lee V.H.Y., Alpini Gianfranco 2006 Cholic acid stimulates bile duct proliferation both in vivo and in vitro via regulation of the expression and secretion of secretin by cholangiocytes: Novel evidence for autocrine/paracrine regulation of cholangiocyte proliferation by secretin, American Association for the study of Liver Diseases, USA. 2006. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lam P.Y., Lee T.O. and Chow B.K.C., Down-regulation of the human secretin gene expression by an atypical nuclear orphan receptor, small heterodimer partner (SHP), Regulatory Peptides. 2006, 130(3): 167. |
| Lam P.Y., Lee T.O., Choi H.S. and Chow B.K.C., Localization of small heterodimer partner (SHP) with the secretin in mouse duodenal cells, Annals New York Academy of Sciences. 2006, 1070: 371-375. |
| Lee T.O., Lee H.Y.V., Yuan Y. and Chow B.K.C., Identification of repressor element 1 in secretin/PACAP/VIP genes, Annals New York Academy of Sciences. 2006, 1070: 388-392. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1, Annals New York Academy of Sciences. 2006, 1070: 393-398. |
| Lee T.O., Lee H.Y.V., Lee P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France. 2005. |
| Lee T.O., Lee H.Y.V., Yuen P.Y. and Chow B.K.C., The role of repressor element 1 silencing transcription factor (REST) in modulating the transcription of human secretin receptor gene, Regulatory Peptides. 2006, 130(3): 167-168. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, Regulatory Peptides. 2006, 130(3): 167. |
| Man C.W.Y., Rosa J., Lee T.O., Lee H.Y.V., Chow B.K.C., Lo K.W., Doxsey S., Wu Z.G., Kwong Y.L., Jin D., Cheung A. and Tsao G.S.W., Latent membrane protein 1 suppresses RASSFIA expression, disrupts microtubule structures and induces chromosomal aberrations in human epithelial cells, Oncogene. 2006, 1-12. |
| Researcher : Lee WWM |
| Project Title: | Analysis of the promoter sequence of rat and mouse testin |
| Investigator(s): | Lee WWM |
| Department: | Zoology |
| Source(s) of Funding: | The Population Council - General Award |
| Start Date: | 07/1999 |
| Abstract: |
| To analysis promoter sequence of rat and mouse testin. |
| Project Title: | Gas6 expression and tyrosine kinase phosphorlation in testicular cell lines |
| Investigator(s): | Lee WWM |
| Department: | Zoology |
| Source(s) of Funding: | The Population Council - General Award |
| Start Date: | 08/1999 |
| Abstract: |
| To study Gas6 expression and tyrosine kinase phosphorlation in testicular cell lines. |
| Project Title: | Induction of germ cell loss by a synthetic occludin peptide and its mechanism of action: an in vivo model to study cell junction formation in the testis |
| Investigator(s): | Lee WWM, Lui WY, Cheng C.Y. |
| Department: | Zoology |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2003 |
| Abstract: |
| N/A |
| Project Title: | Regulation of anchoring junction dynamics in the testis |
| Investigator(s): | Lee WWM, Lui WY, Siu KY, Cheng C.Y. |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2004 |
| Completion Date: | 08/2006 |
| Abstract: |
| To identificate the signaling and scaffolding proteins at the apical ectoplasmic specilizations (ES) that associate with the integrin/laminin, nectin/afadin/ponsin and cadherin/catenin complexes; to characterize the singaling pathways utilized by the actin-based adherens junctions (integrin/lamini, nectin/afadin/ponsin and cadherin/catenin complexes) to regulate ES dynamics. |
| Project Title: | Regulation of anchoring junction dynamics in the testis |
| Investigator(s): | Lee WWM |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Completion Date: | 12/2006 |
| Abstract: |
| N/A |
| Project Title: | Transcriptional regulation of mouse claudin-11 gene in the testis – implications on tight junction dynamics. |
| Investigator(s): | Lee WWM |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 09/2005 |
| Completion Date: | 08/2006 |
| Abstract: |
| 1. To identify the proximal promoter sequence mediating the claudin-11 gene expression. 2. To identify the cis-acting elements within potential promoter sequence (identified in objective 1) that mediate the basal claudin-11 transcription. 3. To characterize the trans-acting factors involved in regulating the basal claudin-11 gene transcription. 4. To characterize the potential signaling pathway that mediates the enhancing/suppressing effects on claudin-11 gene transcription. |
| Project Title: | Testicular tight junctions: molecular architecture, ubiquitination and role of the extracellular matrix |
| Investigator(s): | Lee WWM |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 09/2005 |
| Abstract: |
| N/A |
| Project Title: | Testicular tight junctions: molecular architecture, ubiquitination and role of the extracellular matrix |
| Investigator(s): | Lee WWM, Lui WY, Cheng CY |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2005 |
| Abstract: |
| To identify the integral membrane proteins, scaffolding peripheral proteins, cytoplasmic proteases and protease inhibitors, and signaling molecules at the sites of TJs in the seminiferous epithelium; to characterize the role of extracellular matrix (ECM) and the signaling pathway utilized by TNF-α to regulate TJ dynamics in vitro and I>in vivo. |
| List of Research Outputs |
| Lee W.W.M., Editorial Board, Asian Journal of Andrology. Australia, Blackwell Publishing, 2006. |
| Lee W.W.M., Regional Editor (Asia and Pacific), Reproductive Biology and Endocrinology. BioMed Central, 2006. |
| Lui W.Y., Sze K.L. and Lee W.W.M., Nectin-2 expression in testicular cells is controlled via the functional cooperation between transcription factors of the Sp1, CREB, and AP-1 families, Journal of Cellular Physiology. 2006, 207: 144-157. |
| Lui W.Y. and Lee W.W.M., Regulation of junction dynamics in the testis - Transcriptional and post-translational regulations of cell junction proteins , Molecular and Cellular Endocrinology. 2006, 250: 25-35. |
| Lui W.Y. and Lee W.W.M., Ubiquitination of cell-junction proteins in mammalian testes, The 5th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology, February 7-10 2006, Bangkok, Thailand. 2006. |
| Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases, Journal of Biological Chemistry. 2005, 280(7): 25029-25047. |
| Xia W., Mruk D.D., Lee W.W.M. and Cheng C.Y., Cytokines And Junction Restructuring During Spermatogenesis – A Lesson To Learn From The Testis. , Cytokine & Growth Factor Reviews . 2005, 16: 469-493. |
| Xia W., Mruk D.D., Lee W.W.M. and Cheng C.Y., Differential Interactions between Transforming Growth Factor-b3/TbR1, TAB1, and CD2AP Disrupt Blood-Testis Barrier and Sertoli-Germ Cell Adhesion, Journal of Biological Chemistry. 2006, 281, No. 24: p.16799-16813. |
| Xia W., Wong C.H., Lee P.Y., Lee W.W.M. and Cheng C.Y., Disruption Of Sertoli-germ Cell Adhesion Function In The Seminiferous Epithelium Of The Rat Testis Can Be Limited To Adherens Junctions Without Affecting The Blood-testis Barrier Integrity: An In Vivo Study Using An Androgen Suppression Model. , Journal of Cellular Physiology. 2005, 205: 141-157. |
| Yan H.N.H., Cheng C.Y. and Lee W.W.M., Laminin-333/a6b1 Integrin is a non-basement membrane cell adhesion protein complex at the ectoplasmic specialization of the rat testis, The 45th American Society for Cell Biology Annual Meeting, Dec 10-14 2005, San Francisco, CA, USA. 2005. |
| Researcher : Leung ASP |
| List of Research Outputs |
| Tang J., Leung A.S.P., Leung M.F. and Lim B.L., Hydrolysis of precipitated phytate by three distinct families of phytases, Soil Biology ＆ Biochemistry. 2006, 38: 1316-1324. |
| Researcher : Leung FCC |
| Project Title: | Cloning of the viral genes from the newly identified SARS coronavirus |
| Investigator(s): | Leung FCC |
| Department: | Zoology |
| Source(s) of Funding: | VCO SARS Research Fund |
| Start Date: | 07/2003 |
| Abstract: |
| To clone all the viral gene into vector and these cloned genes will be then be used as reagents by us and other as the first step for investigation. |
| Project Title: | Development of a rapid high throughput RT-PCR assay to detect SARS-CoV |
| Investigator(s): | Leung FCC |
| Department: | Zoology |
| Source(s) of Funding: | VCO SARS Research Fund |
| Start Date: | 07/2003 |
| Abstract: |
| To develop a 96-wells RT-PCR platform assay for the detection of the coronavirus. |
| Project Title: | Molecular cloning of the chicken growth hormone receptor and the molecular characterization of the 5'-flanking region |
| Investigator(s): | Leung FCC |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 10/2003 |
| Abstract: |
| To determine the molecular structure and nucleotide sequence of the chicken GHR gene and the multiple 5' UTR exon 1. |
| Project Title: | Molecular cloning of the chicken growth hormone receptor and the molecular characterization of the 5'-flanking region |
| Investigator(s): | Leung FCC |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 10/2003 |
| Abstract: |
| N/A |
| Project Title: | Isolation and characterization of a PCV2 virus causing postweaning multisystemic wasting syndrome in pigs |
| Investigator(s): | Leung FCC |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To isolate and characterize the PCV2 virus that causes the Postweaning multisystemic wasting syndrome (PMWS) in pigs. |
| Project Title: | Severe acute respiratory syndrome (SARS) coronavirus and apoptosis |
| Investigator(s): | Leung FCC |
| Department: | Zoology |
| Source(s) of Funding: | Research Fund for the Control of Infectious Diseases - Full Grants |
| Start Date: | 01/2005 |
| Abstract: |
| The objective of this proposal is to determine and characterize the SARS coronavirus viral genes (S, M, N and E) induced apoptosis and to further identify and characterize the apoptosis epitopes. |
| List of Research Outputs |
| Chen J., Zhao M., Hui K.H. and Leung F.C.C., Molecular Characterization of Foot-and-mouth Disease Virus in Hong Kong During 2001-2002, Virus Genes. 2006, 32: 139-143. |
| Chow Y.C.K., Yeung Y.S., Hon C.C., Zeng F., Law K.M. and Leung F.C.C., Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells, Federation of European Biochemical Society letters. 2005, 579: 6699-6704. |
| Hon C.C., Lee Y.F., Ching W.K., Ng K.P., Ng Y.W. and Leung F.C.C., Molecular Dating of the Epidemiological Events in Relation to the Emergence of Very Virulent Infectious Bursal Disease Virus, Poultry Science 84(Supp). 2005, 73. |
| Hon C.C., Lam T.Y., Drummond A., Rambaut A., Lee Y.F., Yip C.W., Zeng F., Lam P.Y., Ng P.T.W. and Leung F.C.C., Phylogenetic analysis reveals coincidence of the expansion of very virulent Infectious Bursal Disease Virus and the reassortment of its genome segment B, Journal of Virology. 2006, 80: 8503-8509. |
| Lam T.Y. and Leung F.C.C., Original of PRRS virus: Towards a better understanding beyond the epidemiology, Proceedings of the 2005 International PRRS Symposium, Dec 2-3, 2005, St. Louis, Missouri. 2005, p.63. |
| Li J. and Leung F.C.C., A CRI element is embedded in a novel tandem repeat (HinfI repeat) within the chicken genome, Genome. 2006, 49: 97-103. |
| Li J., Wang Y. and Leung F.C.C., Cloning of Chicken Pre-B-cell Colony-Enhancing Factor 1 (PBEF1) and Characterization of its Spatial Expression, Poultry Science 84(Supp). 2005, 74. |
| Li J., Wang Y., Hon C.C. and Leung F.C.C., Cloning of Smad 1, Smad5 and Smad8 from the Chicken Ovary and Characterization of Their Expression during Chicken Ovarian Development, Poultry Science 84(Supp). 2005, 74. |
| Li Y.Y. and Leung F.C.C., Molecular epidemiology of porcine reproductive and respiratory syndrome virus (PRRSV) in Hong Kong, Proceedings of the 86th Annual Conference of Research Workers in Animal Diseases, St. Louis, Missouri. 2005, p.136. |
| Liang Y., Cui J., Yang G., Leung F.C.C. and Zhang X., Polymorphisms of 5' flanking region of chicken prolactin gene, Dom Animal Endocrinology. 2006, 30: 1-16. |
| Ma C.M. and Leung F.C.C., Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Hong Kong, Proceedings of the 86th Annual Conference of Research Workers in Animal Diseases, St. Louis, Missouri. 2005, p.137. |
| Wang C.Y., Wang Y. and Leung F.C.C., Expression Profiles of Prolactin Receptors during Chicken Embryonic Development, Poultry Science 84(Supp). 2005, 44. |
| Wang C.Y., Wang Y., Li J. and Leung F.C.C., Expression Profiles of the Growth Hormone-releasing Hormone (GHRH) and Growth Hormone-releasing Hormone Receptor (GHRHR) during Chicken Embryonic Pituitary Development, Poultry Science. 2006, 85: 569-576. |
| Wang Y., Wang Z. and Leung F.C.C., A chicken full-length cDNAs database, 2nd International Symposium on Animal Functional Genomics, May 16-19, 2006. |
| Wang Y. and Leung F.C.C., Long inverted repeats in eukaryotic genomes: Recombinogenic motifs determine genomic plasticity, Federation of European Biochemical Society letters. 2006, 580: 1277-1284. |
| Wang Y., Li J. and Leung F.C.C., Regulation of chicken Heparin-binding EGF-like Growth Factor (HB-EGF) Expression by EGF and TGF-a in the cultured ovarian granulosa cells, Poultry Science 84(Supp). 2005, 44. |
| Wang Z., Wang Y. and Leung F.C.C., A database of chicken ESTs alignments with human and mouse full length cDNAs, Proceeding Plant and Animal Genome XIV, San Diego, CA. 2006, p.242. |
| Wong K.W.C., Li Y.Y. and Leung F.C.C., Quantification of porcine cytokine mRNA by real-time RT-PCR, Proceedings of the 86th Annual Conference of Research Workers in Animal Diseases. St. Louis, Missouri . 2005, p.101. |
| Wong T.Y., Hon C.C. and Leung F.C.C., Molecular characterization of IBDV-induced in vitro apoptosis using cDNA microarrays, Proceedings of the Third Symposium on Genetics of Animal of Health, Ames, IA. 2005, p.130. |
| Yip C.W., Xue C. and Leung F.C.C., Identification of Infectious Bursal Disease Virus binding proteins using virus overlay protein binding assays and mass spectrometry, Proceedings of the Third Symposium on Genetics of Animal of Health, Ames, IA. 2005, p.130. |
| Researcher : Leung MF |
| List of Research Outputs |
| Tang J., Leung A.S.P., Leung M.F. and Lim B.L., Hydrolysis of precipitated phytate by three distinct families of phytases, Soil Biology ＆ Biochemistry. 2006, 38: 1316-1324. |
| Researcher : Leung PC |
| List of Research Outputs |
| Hu K. and Leung P.C., Complete, precise, and innocuous loss of multiple introns in the currently intronless, active cathepsin L-like genes, and inference from this event, Molecular Phylogenetics and Evolution. 2006, 38: 685-696. |
| Liu S.Y.K., Tsang J.S.H., Leung P.C., Chow B.K.C. and Wan J.M.F., Proteomics of Macrophage; an Approach to Understand Lipopolysaccharide-challenged inflammation, Molecular & Cellular Proteomics 4.8:S231, HUPO 4th Annual World Congress, August 28-September 1, 2005, Munich. 2005. |
| Wang Y., Leung P.C., Qian P. and Gu J., Antibiotic resistance and plasmid profile of environmental isolates of Vibrio species from Mai Po Nature Reserve, Hong Kong, Ecotoxicology. 2006, 15: 371-378. |
| Researcher : Li ETS |
| Project Title: | Nutritional benefits of dietary fiber supplementation in hospitalized geriatrics |
| Investigator(s): | Li ETS, Lui S.S.H., Dai D.L.K., Chiu S., Chan V., Dai D.L.K. |
| Department: | Zoology |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 09/1997 |
| Abstract: |
| To improve gentle bowel fitness, relieve constipation and improve serum lipid profile of institutionalized geriatric patients via dietary fiber supplementation. |
| Project Title: | An evaluation on the antioxidant effects of Lycium barbarum L. and its supplementation on cataract formation in rats |
| Investigator(s): | Li ETS |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To systematically examine the antioxidant properties of Lycium barbarum L. and evaluate the effect of its supplementation on cataract development in rats. |
| Project Title: | Dietary bitter melon reduces adiposity and enhances insulin sensitivity: a role for peroxisome proliferator-activated receptors? |
| Investigator(s): | Li ETS |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 12/2003 |
| Abstract: |
| Determine the effect of bitter melon supplementation on adiposity, glucose tolerance and endocrine profile of insulin resistant rats; determine the involvement of peroxisome proliferators-activated receptors and adipocyte-derived peptides; determine the involvement of sympathetic activity and uncoupling proteins from brown and white adipocytes, and skeletal muscle. |
| Project Title: | Dietary bitter melon reduces adiposity and enhances insulin sensitivity: a role for peroxisome proliferator-activated receptors? |
| Investigator(s): | Li ETS |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 12/2003 |
| Abstract: |
| N/A |
| Project Title: | Anti-cataract effects of Lycium barbarum L and Momordica charantia |
| Investigator(s): | Li ETS |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To systematically examine the antioxidant properties of Lycium barbarum L. and Momordica charantia and evaluate the effects of supplementation on cataract development in rats. |
| Project Title: | Anti-obesity effects of bitter melon (Momordica charantia) |
| Investigator(s): | Li ETS |
| Department: | Zoology |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2005 |
| Completion Date: | 06/2006 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Chan L.Y., Chen Q., Go A.G.G., Lam E.K.Y. and Li E.T.S., Reduced Adiposity in Bitter Melon (Momordica charantia) Fed Rats Is Associated with Increased Lipid Oxidative Enzyme Activities and Uncoupling Protein Expression., Journal of Nutrition. 2005, 135: 2517-2523. |
| Xu R., Ma H., Li H., Sham M.H., Li E.T.S., Tam P.K.H. and Lam K.S.L., Oral viral vector gene delivery, In: Redberry GW (ed). Trends in Gene Therapy Research. New York, U.S.A., Nova Sciecen Publishers, Inc., 2005, 191-221. |
| Researcher : Li J |
| List of Research Outputs |
| Li J. and Leung F.C.C., A CRI element is embedded in a novel tandem repeat (HinfI repeat) within the chicken genome, Genome. 2006, 49: 97-103. |
| Li J., Wang Y. and Leung F.C.C., Cloning of Chicken Pre-B-cell Colony-Enhancing Factor 1 (PBEF1) and Characterization of its Spatial Expression, Poultry Science 84(Supp). 2005, 74. |
| Li J., Wang Y., Hon C.C. and Leung F.C.C., Cloning of Smad 1, Smad5 and Smad8 from the Chicken Ovary and Characterization of Their Expression during Chicken Ovarian Development, Poultry Science 84(Supp). 2005, 74. |
| Wang C.Y., Wang Y., Li J. and Leung F.C.C., Expression Profiles of the Growth Hormone-releasing Hormone (GHRH) and Growth Hormone-releasing Hormone Receptor (GHRHR) during Chicken Embryonic Pituitary Development, Poultry Science. 2006, 85: 569-576. |
| Wang Y., Li J. and Leung F.C.C., Regulation of chicken Heparin-binding EGF-like Growth Factor (HB-EGF) Expression by EGF and TGF-a in the cultured ovarian granulosa cells, Poultry Science 84(Supp). 2005, 44. |
| Researcher : Li J |
| List of Research Outputs |
| Li J. and Leung F.C.C., A CRI element is embedded in a novel tandem repeat (HinfI repeat) within the chicken genome, Genome. 2006, 49: 97-103. |
| Li J., Wang Y. and Leung F.C.C., Cloning of Chicken Pre-B-cell Colony-Enhancing Factor 1 (PBEF1) and Characterization of its Spatial Expression, Poultry Science 84(Supp). 2005, 74. |
| Li J., Wang Y., Hon C.C. and Leung F.C.C., Cloning of Smad 1, Smad5 and Smad8 from the Chicken Ovary and Characterization of Their Expression during Chicken Ovarian Development, Poultry Science 84(Supp). 2005, 74. |
| Wang C.Y., Wang Y., Li J. and Leung F.C.C., Expression Profiles of the Growth Hormone-releasing Hormone (GHRH) and Growth Hormone-releasing Hormone Receptor (GHRHR) during Chicken Embryonic Pituitary Development, Poultry Science. 2006, 85: 569-576. |
| Wang Y., Li J. and Leung F.C.C., Regulation of chicken Heparin-binding EGF-like Growth Factor (HB-EGF) Expression by EGF and TGF-a in the cultured ovarian granulosa cells, Poultry Science 84(Supp). 2005, 44. |
| Researcher : Li W |
| List of Research Outputs |
| Wong A.O.L., Li W., Leung C.Y., Huo L. and Zhou H., Pituitary adenylate cyclase-activating polypeptide (PACAP) as a growth hormone (GH)-releasing factor in grass carp: I. Functional coupling of cAMP- and Ca2+/ calmodulin-dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells. , Endocrinology. USA, The Endocrine Society, 2005, 146: 5407-5424. |
| Researcher : Li YY |
| List of Research Outputs |
| Li Y.Y. and Leung F.C.C., Molecular epidemiology of porcine reproductive and respiratory syndrome virus (PRRSV) in Hong Kong, Proceedings of the 86th Annual Conference of Research Workers in Animal Diseases, St. Louis, Missouri. 2005, p.136. |
| Wong K.W.C., Li Y.Y. and Leung F.C.C., Quantification of porcine cytokine mRNA by real-time RT-PCR, Proceedings of the 86th Annual Conference of Research Workers in Animal Diseases. St. Louis, Missouri . 2005, p.101. |
| Researcher : Lie Ken Jie MSF |
| List of Research Outputs |
| Lie Ken Jie M.S.F., In Search of NOx Reducing Agents for Biodiesel, 96th Annual Meeting of the American Oil Chemists Society, Salt Lake City, June 2005. |
| Lie Ken Jie M.S.F., Plenary lecture: Lipid chemistry at the cutting edge, American Oil Chemists Society Annual Meeting, Salt Lake City, June 2005. 2005. |
| Lie Ken Jie M.S.F., Supelco-Nick Pelick AOCS Research Award 2005, American Oil Chemists' Society, Champaign, Illinois. 2005. |
| Researcher : Lim BL |
| Project Title: | Studies on Bacillus phytases |
| Investigator(s): | Lim BL |
| Department: | Zoology |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 09/2000 |
| Abstract: |
| To close the genes of Bacillus phytases; to overexpress Bacillus phytases and to characterize their biochemical properties; to carry out agricultural applications of Bacillus phytases. |
| Project Title: | Utilization of soil organic phosphorus by genetically modified plants |
| Investigator(s): | Lim BL, Koyama H. |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2004 |
| Abstract: |
| To confirm whether the enhanced growth performance of the GM line is attributed to the utilization of soil IHIP by the secreted [beta]-propella phytase; to examine whether the GM plants have lower P requriement than the null lines and the wildtype; to examine whether the GM plants can utilized various salt forms of IHPs in soil. (Fe-phytate, Ca-phytate, Al-phytate); to examine the stability and the activity of the secreted phytase in soil ; to compare the abilities of 3 classes of phytases (BPP, HAP and PAP) in hydrolyzing IHP adsorbed to various soil components (A1, Fe and Ca precipitates, clays, etc); to examine the effects of organic acids (citrate, malate, oxalate) on the activities of the three classes of phytases (BPP, HAP and PAP); to create a phytase secreting Arabidopsis line that has elevated citrate acid secretion from root and compare its growth performance with the line that solely secretes phytase. |
| Project Title: | Utilization of soil organic phosphorus by genetically modified plants |
| Investigator(s): | Lim BL |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Abstract: |
| N/A |
| Project Title: | The roles of plant purple acid phosphatases in phytate assimilation |
| Investigator(s): | Lim BL |
| Department: | Zoology |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2005 |
| Completion Date: | 06/2006 |
| Abstract: |
| N/A |
| Project Title: | Expression and characterization of PAPs 10, 15 and 18 from Arabidopsis thaliana |
| Investigator(s): | Lim BL |
| Department: | Zoology |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 09/2005 |
| Abstract: |
| For many years, plants have been regarded as incapable of assimilating phytate, the major form of organic phosphorus in soil, by their roots. Our recent data showed that wild type tobacco could grow normal in sand culture supplemented with magnesium phytate salts, but not in sand cultures supplemented with Na-phytate, Al-phytate and Mn-phytate. The results implied that plant roots could secrete an enzyme which can hydrolyze Mg-phytate and we postulate it as a member of purple acid phophatase. Genome analysis shows that there are 29 PAP-like genes in the whole genome of Arabidopsis. We carried out a phylogenetic analysis with 50 plant PAP a.a. sequences and grouped them into several subgroups. In this project, 3 representative Arabidopsis PAP genes (AtPAPs 10, 15 and 18) from each subgroups are selected for overexpression in a baculovirus expression system. The enzymes will be purified and their biochemical properties, including substrate specificities, Km, Kcat, pH and temperature profiles, will be studied. Since most of the reported PAPs did not exhibit phytase activity, we wish to verify whether one of these AtPAPs has phytate-hydrolyzing ability. This enzyme will have great potential in agriculture application. The recombinant proteins will also be used to raise specific antisera from rabbits. Our previous works detected phytase activity in plant growth media after growing the plant for 30 days. The activity was presumably secreted from the root. To identify the identity of the phytase secreted from plant root, specific antibodies will be used for western blotting studies and in enzyme inhibition assays. |
| List of Research Outputs |
| Chan W.L., Lung S.C. and Lim B.L., Properties of beta-propeller phytase expressed in transgenic tobacco , Protein expression and purification. 2005, 46: 100-106. |
| Cheng C.W. and Lim B.L., Beta-propeller phytases in the aquatic environment, Archives in Microbiology. 2005, 185: 1-13. |
| Lim B.L., Beta-propeller phytases in the aquatic environment: characterization of a novel phytase from Shewanella oneidensis MR-1, Symposium lecture, Bouyoucos Conference: Inositol Phosphates in the Soil-Plant-Animal system , Sun Valley, Idaho, USA, August 21-24, 2005.. 2005. |
| Lung S.C. and Lim B.L., Assimilation of phytate-phosphorus by the extracellular phytase activity of tobacco (Nicotiana tabacum) is affected by the availability of soluble phytate, Plant and Soil. 2006, 279: 187-199. |
| Tang J., Leung A.S.P., Leung M.F. and Lim B.L., Hydrolysis of precipitated phytate by three distinct families of phytases, Soil Biology ＆ Biochemistry. 2006, 38: 1316-1324. |
| Researcher : Liu SYK |
| List of Research Outputs |
| Liu S.Y.K., Tsang J.S.H., Leung P.C., Chow B.K.C. and Wan J.M.F., Proteomics of Macrophage; an Approach to Understand Lipopolysaccharide-challenged inflammation, Molecular & Cellular Proteomics 4.8:S231, HUPO 4th Annual World Congress, August 28-September 1, 2005, Munich. 2005. |
| Researcher : Lu B |
| List of Research Outputs |
| Lu B., Baga M., Sun M., Corke H. and Chibbar R.N., Isolation, characterization, and expression of starch synthase genes from grain amaranth, The 12th Annual Life Sciences Research Conference, March 17 - 18, 2005, University of Saskastchewan, Canada. 2005. |
| Researcher : Lui WY |
| Project Title: | Transcriptional regulation of mouse nectin-2 gene in Sertoli cells |
| Investigator(s): | Lui WY, Lee WWM |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Completion Date: | 10/2005 |
| Abstract: |
| To explore the regulatory mechanism of nectin-2 gene expression in the testis. |
| List of Research Outputs |
| Lui W.Y., Sze K.L. and Lee W.W.M., Nectin-2 expression in testicular cells is controlled via the functional cooperation between transcription factors of the Sp1, CREB, and AP-1 families, Journal of Cellular Physiology. 2006, 207: 144-157. |
| Lui W.Y. and Lee W.W.M., Regulation of junction dynamics in the testis - Transcriptional and post-translational regulations of cell junction proteins , Molecular and Cellular Endocrinology. 2006, 250: 25-35. |
| Lui W.Y. and Lee W.W.M., Ubiquitination of cell-junction proteins in mammalian testes, The 5th Intercongress Symposium of the Asia and Oceania Society for Comparative Endocrinology, February 7-10 2006, Bangkok, Thailand. 2006. |
| Researcher : Lung SC |
| List of Research Outputs |
| Chan W.L., Lung S.C. and Lim B.L., Properties of beta-propeller phytase expressed in transgenic tobacco , Protein expression and purification. 2005, 46: 100-106. |
| Lung S.C. and Lim B.L., Assimilation of phytate-phosphorus by the extracellular phytase activity of tobacco (Nicotiana tabacum) is affected by the availability of soluble phytate, Plant and Soil. 2006, 279: 187-199. |
| Researcher : Ma CM |
| List of Research Outputs |
| Ma C.M. and Leung F.C.C., Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Hong Kong, Proceedings of the 86th Annual Conference of Research Workers in Animal Diseases, St. Louis, Missouri. 2005, p.137. |
| Researcher : Ma HT |
| List of Research Outputs |
| Ma H.T. and Chan S.M., Characterization of the C-type lectin cDNA from the shrimps Penaeus monodon and Litopenaeus vannamei, SICB Annual Meeting January 4-8, 2006 Buena Vista Palace Resort and Spa in the Walt Disney World Resort Orlando, FL. 2006. |
| Researcher : Mak ASC |
| List of Research Outputs |
| Tiu H.K., Hui J.H.L., Mak A.S.C., He J.G. and Chan S.M., Equal contribution of hepatopancreas and ovary to the production of vitellogenin (PmVg1) transcripts in the tiger shrimp, Penaeus monodon, Aquaculture. 2006, 254: 666-674. |
| Researcher : Mak C |
| List of Research Outputs |
| Wu W.K., Mak C. and Ko R.C.C., Cloning and characterization of the Cu/Zn superoxide dismutase of Trichinella pseudospiralis, Parasitology Research. 2005, 98: 281-287. |
| Researcher : Man KNM |
| List of Research Outputs |
| Man K.N.M., Philipsen S. and Tan-Un K.C., Localization of cytoglobin, a novel protein, in lung tissues, European Respiratory Society Congress, September 2005, European Respiratory Journal. 2005, 26(Supp). 49, 551s. |
| Researcher : Mei J |
| List of Research Outputs |
| Mei J., Zhang Y.Q., Wang T. and Xu R.J., Effects of oral ingestion of colostrum on intestinal expression of TGF-beta receptors in the newborn pig, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S89. |
| Researcher : Mruk DD |
| List of Research Outputs |
| Xia W., Mruk D.D., Lee W.W.M. and Cheng C.Y., Cytokines And Junction Restructuring During Spermatogenesis – A Lesson To Learn From The Testis. , Cytokine & Growth Factor Reviews . 2005, 16: 469-493. |
| Xia W., Mruk D.D., Lee W.W.M. and Cheng C.Y., Differential Interactions between Transforming Growth Factor-b3/TbR1, TAB1, and CD2AP Disrupt Blood-Testis Barrier and Sertoli-Germ Cell Adhesion, Journal of Biological Chemistry. 2006, 281, No. 24: p.16799-16813. |
| Researcher : Ng SM |
| Project Title: | Molecular cloning and functional characterization of the human cell cycle related kinase-interacting proteins |
| Investigator(s): | Ng SM, Lin MC |
| Department: | Chemistry |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 12/2005 |
| Abstract: |
| 1. To clone the genes encoding the proteins that specifically interact with CCRK in a yeast two-hybrid screening. 2. To elucidate the functional roles of the CCRK-interacting proteins in glioblastoma carcinogenesis. |
| List of Research Outputs |
| Researcher : Ngai HYH |
| List of Research Outputs |
| Ngai H.Y.H., Sit W.H., Jiang P., Thongboonkerd V. and Wan J.M.F., Comparative Proteomics Analysis Reveals Haptoglobin as a Possible Novel Marker in Human Membranous Nephropathy, Molecular ＆ Cellular Proteomics, Human Proteome Organisation, 5th Annual World Congress, October 28 - November 1, 2006, Long Beach, California. 2006, 5.10: 496. |
| Researcher : Ngan ESW |
| Project Title: | Sonic hedgehog (Shh) signaling in the enteric neural crest cells |
| Investigator(s): | Ngan ESW, Sham MH, Lui VCH, Tam PKH |
| Department: | Surgery |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2005 |
| Completion Date: | 12/2006 |
| Abstract: |
| The main objectives of this project are: 1) To investigate the effect of Shh on the expression profiles of BMP-2 and BMP-4 in mouse embryonic gut. 2) To study the interplay between Shh/BMPs and GDNF signaling in NCC migration. The results of this basic research would provide better understanding of the process of ENS development. This data could further extend the knowledge base regarding the etiology and our understanding of Hirschsprung's disease in human. |
| Project Title: | Novel Function(s) of Prokineticins in Neural Crest Stem Cells |
| Investigator(s): | Ngan ESW, Lui VCH, Tam PKH |
| Department: | Surgery |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2006 |
| Abstract: |
| The mammalian enteric nervous system (ENS) is derived from the neural crest cells (NCC) which enter the foregut and colonize the entire wall of gastrointestinal tract (1). In mice, NCCs reach foregut at approximately embryonic day 9.4 (E9.5) and finish the colonization by ~E14.5. Numerous gut mesenchyme/mucosa-derived factors and their receptors on NCC are found to be crucial for NCC proliferation, differentiation and migration. They are glial cell-line derived neurotrophic factor (GDNF) and its receptors (RET, GFRA1)(2); endothelin 3 (EDN3) and its receptor (EDNRB)(3); sonic hedgehog (Shh) and its receptor (Ptc)(4). A delicate balance between these signals is a prerequisite for the proper regulation of NCCs during ENS development. Failure to completely colonize the gut results in the absence of enteric ganglia as seen in Hirschsprung’s disease in human.. Prokineticin –1 and -2 (Prok-1 and Prok-2), also referred to endocrine gland derived vascular endothelial growth factor (EG-VEGF) and Bv8, respectively, are structurally related and belong to a newly identified family of secreted proteins, AVIT protein family. These proteins are distributed widely in mammalian tissues. Prok-1 mRNA expression has been described in a variety of tissues, in steroidogenic glands such as the ovary, testis, adrenal gland and placenta, but also in the brain, colon, skeletal muscle, small intestine, spleen, thymus liver, bladder, prostate and uterus(5,6) . Prok-2, on the other hand, shows the highest expression in testis, colon, brain and peripheral blood leukocytes(7,8). These two prokineticins are known to bind to two closely related G protein-coupled receptors, PK-R1 and PK-R2. Receptor activation leads to mobilization of calcium, stimulation of phosphoinositide turnover, and activation of p44/p42 MAPK signaling pathways. Within distinct contexts, the receptors are likely differentially regulated and distinct expression patterns of Prok receptors were observed in various tissues. In the adrenal gland, only PK-R1 was detected. In the ovary, follicular cells predominantly express PK-R1, whereas corpus luteum-derived cells express high levels of both PK-R1 and PK-R2. Similarly, these two receptors are also co-existing in endometrium, but at different expression level. Therefore, it is believed that prokineticins may have different angiogenic as well as non-angiogenic functions in different tissues via acting on either PK-R1 or PK-R2. However, the exact role(s) of PK-R1 and PK-R2 remains unclear. A new appreciation for the intricacy of peptidergic GPCRs is developing, as the ligands for orphan receptors are identified and as distinct signaling and functional responses for multiple ligands and oligomerized receptors are demonstrated. Thus, the distinct or overlapping expression of prokineticins and the possible heteroligomerization of receptors may increase the functional complexity of this system (8,9). It is currently known that prokineticins possess diverse biological functions: they promote angiogenesis in ovary and testis; induce proliferation, migration and fenestration of endothelial cells derived from adrenal gland ; support neuronal survival; cause hyperalgesia of skin; promote contraction of gastrointestinal smooth muscle; and control pain sensation and behavioral circadian rhythms(8,9). More recently, these two receptors were also found in human and mouse hematopoietic stem cells and specific mature blood cells, including lymphocytes. Prokineticins can modulate growth, survival, and function of cells of the innate and adaptive immune systems, possibly through autocrine or paracrine signaling mechanisms(7). Given the reported expression patterns and activities of the mammalian orthologs in brain, reproductive tract, and cells of the immune system, the molecules seem to have subserved similar functions across evolution. In addition to being the angiogenic factors, prokineticins would be the universal survival/mitogenic factors for various cells including endothelial cells, neuronal cells, lymphocytes as well as the hematopietic stem cells. Noteworthy, prokineticins and their receptors were detected in mouse embryo as early at E7, the role(s) of these factors during embryonic development still remains to be disclosed. Our preliminary data: It is known that prokineticin-1 is expressed in mouse and human small intestine and colon. Using antibody specific to Prok-1 to perform the immunohistochemistry study, we found that Prok-1 protein is expressed in the mucosa and enteric neuronal plexus of the gut in the adult mouse. The presence of Prok-1 in neuronal plexus was subsequently confirmed by colocalization study with the neuronal marker, Tuj1 (Appendix I, Figure 1g-i). In embryonic stage, it is highly expressed in the mucosa (E12.5 and E15.5) and forms a concentration gradient across the radius of the embryonic gut (Appendix I, Figure 1a-f). Subsequent RT-PCR analysis also showed that NCCs do not express endogenous Prok-1 (data not shown). A physiological effect of Prok-1 is dependent on the presence of the ligand and the receptor, so we have also examined the expression of the receptors, PK-R1 and PK-R2. Expression study using RT-PCR showed that the PK-R1, but not PK-R2, is expressed in NCCs isolated from E11.5 embryonic gut (Appendix I, Figure 2A). Localization of PK-R1 receptor using in situ hybridization further demonstrated the existence of PK-R1 in the NCCs and NCC derived enteric neurons of the embryonic guts (E10.5-E17.5). The signal is specific because there is no fluorescent signal detected when sense probe was used (Ctrl, Appendix I, Figure 2B). Taken together, it is believed that Prok-1, probably like GDNF, EDN3 and Shh, acts as mesenchyme/mucosa-derived factors and mediate the proliferation or differentiation of NCCs during ENS development. On the other hand, it may also act as a survival factor for the enteric neurons in the adult gut, via an autocrine/paracrine mechanism. Thus, we hypothesize that Prok-1 provides an additional layer of signaling refinement to maintain survival/proliferation of NCCs and mature neurons of the developing and mature ENS, respectively. The aim of this proposed project is to elucidate the physiological role(s) of prokineticins in ENS and during its development. Objective 1: To study the temporal and spatial expression patterns of prokineticins (Prok-1 and Prok-2) and their receptors in mouse gut during ENS development. The information obtained would give a hint to delineate the potential physiological functions of these molecules. Objective 2: To elucidate the mitogenic/neuroprotective properties of prokineticins on NCCs and NCC derived neurons. The functions of prokineticins in NCCs will be examined directly using the in vitro NCC culture system. Objective 3: To examine the effect of prokineticins on the differentiation capacity of NCCs. Reference 1. D. Newgreen, H. M. Young, Pediatr. Dev. Pathol. 5, 329 (2002). 2. A. Barlow, E. de Graaff, V. Pachnis, Neuron 40, 905 (2003). 3. G. M. Kruger et al., Neuron 40, 917 (2003). 4. M. Fu, V. C. Lui, M. H. Sham, V. Pachnis, P. K. Tam, J. Cell Biol. 166, 673 (2004). 5. J. LeCouter et al., Nature 412, 877 (2001). 6. E. S .Ngan et al., Endocrinology Oct 6; [Epub ahead of print] (2005) . 7. J. LeCouter, C. Zlot, M. Tejada, F. Peale, N. Ferrara, Proc. Natl. Acad. Sci. U. S. A 101, 16813 (2004). 8. A. Kaser, et al., EMBO reports 4,469 (2003) 9. J. LeCouter, R. Lin, N. Ferrara Ann. N.Y. Acad. Sci 1014: 50(2004). |
| List of Research Outputs |
| Researcher : Pan M |
| List of Research Outputs |
| Pan M. and Xu R.J., Effects of gonadic hormone and phytoestrogen on fetal rat hippocampal neuron proliferation in vitro, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 Nov - 3 Dec 2005. 14 (Suppl) S61. |
| Researcher : Pon YL |
| List of Research Outputs |
| Pon Y.L., Auersperg N. and Wong A.S.T., Gonadotropins induce loss of N-cadherin, increased transcriptional activity of b-catenin, and apoptosis in human ovarian surface epithelial cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.482 (Abstract No. P2-345). 2006. |
| Researcher : Sit WH |
| List of Research Outputs |
| Chan L.L., Sit W.H., Lam P.K.S., Hsieh D.P.H.T., Hodgkiss I.J., Wan J.M.F., Ho A.Y.T., Choi N.M.C., Wang D.Z. and Dudgeon D., Identification and characterization of a "biomarker of toxicity" from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae), Proteomics. 2006, 6: 654-666. |
| Lee C.L., Sit W.H. and Wan J.M.F., Cyclosporine A-like Immunomodulatory Benefits of Polysaccharopeptide (PSP) Derived from Coriolus Versicolor in Activated Human T-cells, Inflammation research 54(supp. 2):S130, 7th World Congress of Inflammation, Australia. 2005. |
| Ngai H.Y.H., Sit W.H., Jiang P., Thongboonkerd V. and Wan J.M.F., Comparative Proteomics Analysis Reveals Haptoglobin as a Possible Novel Marker in Human Membranous Nephropathy, Molecular ＆ Cellular Proteomics, Human Proteome Organisation, 5th Annual World Congress, October 28 - November 1, 2006, Long Beach, California. 2006, 5.10: 496. |
| Wan J.M.F., Hui P.Y. and Sit W.H., Induction of S phase cell arrest and caspase activation by polysaccharide peptide (PSP) isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cells, 10th World Congress on Advances in Oncology and 8th International Symposium on Molecular Medicine, 2005 Greece. 2005. |
| Wan J.M.F., Sit W.H., Lee C.L., Fu H.M.K. and Chan D.K.O., Protection of lethal toxicity of endotoxin by Salvia miltiorrhiza BUNGE is via reduction in tumor necrosis factor alpha release and liver injury, International Immunopharmacology. 2006, 6: 750-758. |
| Researcher : Siu KY |
| Project Title: | Ectoplasmic specialization dynamics in the testis is regulated by focal adhesion complex-mediated signaling pathways |
| Investigator(s): | Siu KY, Lee WWM |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To investigate the downstream signaling pathway(s) underneath integrin and FAK that contribute to the regulation of ES dynamics. |
| Project Title: | p21-activated kinases (Paks) in ovarian and endometrial cancers |
| Investigator(s): | Siu KY, Cheung ANY |
| Department: | Pathology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 01/2006 |
| Abstract: |
| The purpose of the proposed investigation is to characterize the expression levels and the signaling pathways of Paks in ovarian and endometrial cancers in an attempt to explore the roles of Paks in their carcinogenesis, with potential impacts on the development of targeted anticancer therapies. Key Issues and Problems being addressed: Ovarian and endometrial cancers are two common gynecological cancers. In 2002, ovarian cancer ranked eighth among the major causes of cancer death in Hong Kong. Endometrial cancer is the most common gynecological cancer worldwide. To have a greater chance to cure, it is important to define the staging of cancers at the first diagnosis. Recent studies in our laboratories have reported several tumor suppressor genes as potential cancer markers, which play a vital role in gynecological malignancies [1-5]. The high mortality rate of cancers is related to metastasis, which is a process involving alternation in signaling pathways, increased directional cell migration and enhanced cell survival [6-8]. There is accumulating evidence that actin cytoskeleton reorganization is an essential step for cell migration, anchourage-independent growth, and invasion [7]. Members of small GTPases of the Rho family (RhoA, Rac1, and Cdc42) have been found to be an important regulator for cytoskeleton reorganization [7]. RhoA is involved in stress fiber formation, Rac1 is for lamellipodia formation and Cdc42 is for filopodia formation [6, 9]. In mammalian cells, p21-activated serine/threonine kinases (Paks) were initially identified as an effector molecules of Rac1 and Cdc42. Consequently, they were found to be activated by both GTPase-dependent and -independent manners [6, 9]. To date, six members of Paks have been found. They can be classified into two subgroups: Group 1, consisting of Pak1 (Pak), Pak2 (Pak), and Pak3 (Pak), which share >90% homology in their kinase domain and 73% overall homology in their entire sequence; and Group 2, consisting of Pak4-6, which share only ~50% homology with the Pak1 kinase domain [6, 9]. For GTPase-dependent activation manner, Paks bind with Rac1 and Cdc42 in their active, GTP bound state, inhibiting their intrinsic GTPase activity and leading to Paks autophosphorylation. Once phosphorylated, they dissociate from Rac1/Cdc42 to phosphorylate downstream effectors. Although all Paks can bind to GTPases, only Group I Paks can be activated through this binding [6, 9]. In addition, Paks can be activated through a variety of GTPase-independent mechanisms, including direct phosphorylation by other kinases, such as protein kinase B (PKB, also know as Akt) under the influence of lipid signaling involving PI 3-kinase [6, 9, 10]. Due to their complicated activation processes and the presence of multiple downstream effectors, Paks play an important role in a wide range of cellular processes, including cytoskeletal reorganization, cell motility, apoptosis, cell cycle progression, and cell transformation, all of which are required for cancer progression [6, 10, 11]. The differential expression of Paks in ovarian and endometrial cancers is poorly defined. Only Pak1 has been found to be overexpressed in ovarian cancers [12]. Worst yet, the upstream mediators and the downstream signaling pathway(s) of Paks that leads to cancer progression in ovarian and endometrial cancers are still unknown. References (Publications from our group): 1.* Chan QKY, Khoo US, Ngan HYS, Yang CQ, Xue WC, Chan KYK, Chiu PM, Ip PPC, Cheung ANY. Single Nucleotide Polymorphism of Pi-class Glutathione-S-Transferase and Susceptibility to Endometrial Carcinoma. Clin. Cancer Res. 2005; 11: 2981-2985. 2.* Chan KYK, Ozcelik H, Cheung ANY, Ngan HYS, Khoo US. Epigenetic factors controlling the BRCA1 and BRCA2 genes in sporadic ovarian cancer. Cancer Research 2002; 63: 4151-4156. 3.* Shen DH, Chan KYK, Khoo US, Ngan HYS, Xue WC, Chiu PM, Ip PPC, Cheung ANY. Epigenetic and genetic alterations of p33ING1b in ovarian cancer. Carcinogenesis 2005; 26: 855-863. 4.* Chan QKY, Khoo US, Chan KYK, Ngan HYS, Li SS, Chiu PM, Man LS, Ip PPC, Xue WC, Cheung ANY. Promoter Methylation and Differential Expression of pi-class Glutathione-S-Transferase in Endometrial Carcinoma. Journal of Molecular Diagnostics 2005; 7: 8-16. 5. The Hong Kong Cancer Registry. Department of Health Annual Report 2001/2002. Hong Kong, Department of Health, Hospital Authority. 2000. 6. Bokoch GM. Biology of the p21-activated kinases. Annu. Rev. Biochem. 2003; 72: 743-781. 7. Hall A. Rho GTPases and the actin cytoskeleton. Science 1998; 279: 509-514. 8. Vadlamudi RK, Kumar R. P21-activated kinases in human cancer. Cancer Metastasis Rev. 2003; 22: 385-393. 9. Hofmann C, Shepelev M, Chernoff J. The genetics of Pak. J. Cell Sci. 2004; 117: 4343-4354. 10.* Siu MKY, Wong CH, Lee WM, Cheng CY. Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases. J. Biol. Chem. 2005; 280: 25029-25047. 11. Kumar R, Hung MC. Signaling intricacies take center stage in cancer cells. Cancer Res. 2005; 65: 2511-2515. 12. Schraml P, Schwerdtfeger G, Burkhalter F, Raggi A, Schmidt D, Ruffalo T, King W, Wilber K, Mihatsch MJ, Moch H. Combined array comparative genomic hybridization and tissue microarray analysis suggest PAK1 at 11q13.5-q14 as a critical oncogene target in ovarian carcinoma. Am. J. Pathol. 2003; 163: 985-992. |
| List of Research Outputs |
| Chu Y.S.J., Lee T.O., Siu K.Y. and Chow B.K.C., The Secretin/Pituitary Adenylate Cyclase-Activating Polypeptide/Vasoactive Intestinal Polypeptide Superfamily in the Central Nervous System, Central Nervous System Agents in Medicinal Chemistry. 2006, 6: 27-57. |
| Siu K.Y., Sham M.H. and Chow B.K.C., Secretin is widely expressed during mouse embryonic development, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Siu K.Y., Sham M.H. and Chow B.K.C., Secretin is widely expressed during mouse embryonic development, Regulatory Peptides. 2006, 130(3): 176. |
| Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases, Journal of Biological Chemistry. 2005, 280(7): 25029-25047. |
| Siu K.Y., Sham M.H. and Chow B.K.C., The prenatal expression of secretin receptor, Annals New York Academy of Sciences. 2006, 1070: p561-565. |
| Researcher : Siu KY |
| List of Research Outputs |
| Chu Y.S.J., Lee T.O., Siu K.Y. and Chow B.K.C., The Secretin/Pituitary Adenylate Cyclase-Activating Polypeptide/Vasoactive Intestinal Polypeptide Superfamily in the Central Nervous System, Central Nervous System Agents in Medicinal Chemistry. 2006, 6: 27-57. |
| Siu K.Y., Sham M.H. and Chow B.K.C., Secretin is widely expressed during mouse embryonic development, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Siu K.Y., Sham M.H. and Chow B.K.C., Secretin is widely expressed during mouse embryonic development, Regulatory Peptides. 2006, 130(3): 176. |
| Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases, Journal of Biological Chemistry. 2005, 280(7): 25029-25047. |
| Siu K.Y., Sham M.H. and Chow B.K.C., The prenatal expression of secretin receptor, Annals New York Academy of Sciences. 2006, 1070: p561-565. |
| Researcher : Sun M |
| Project Title: | Comparative studies of selected medicinal plants from China and India: phylogenetic relationships, population genetics, and conservation |
| Investigator(s): | Sun M, Corke H, Cai Y |
| Department: | Zoology |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2005 |
| Completion Date: | 12/2006 |
| Abstract: |
| Folk medicinal herbs are commonly used for treatment of many diseases. Both China and India are among the countries rich in medicinal plant resources. During long-term folk practices, the two different cultures have each identified a range of medicinal plants. For example, of the recorded 27,100/17,000 native species in the flora of China/India, 11,146/7500 species are reported to have some medicinal values, respectively (Hamilton 2002). Still many locally available species are neglected or under-utilized because of the limitations of traditional approaches, such as random selection or the "trial and error" method, in the discoveries of medicinal plants. Cross-cultural investigations will promote further exploration of local flora for identification of new species of medicinal properties to facilitate the discovery of new drugs. For example, on the lists of important or national priority medicinal plants in India, many of the species have numerous congeners native to China. Although in some genera the same species are utilized as medicinal herbs in both cultures, more frequently this is not the case. Therefore, new medicinal species in the flora of China/India could be easily identified simply by reference to the recognized medicinal species in the other culture, although whether the plants with identical Latin names in different flora are actually the same species need to be confirmed. Furthermore, phylogenetic analysis can be performed for the genera/families containing well-known medicinal species both within and between cultures, in order to identify their closest wild relatives which are more likely possess the same or similar bioactive compounds. The traditional approaches to medicinal herb identification and classification are largely dependent on morphological and anatomical characteristics, but many of such characteristics are affected by environmental and developmental factors. Modern chemical analytical techniques and tools have been extensively used for characterization and quantification of bioactive components in medicinal plants. Plant species or genera with close relationships usually contain similar chemical compounds. Recent advances in molecular techniques render DNA-based assays more advantageous than both morphological and chemical approaches in resolving phylogenetic relationships at various taxonomic levels. The molecular genetic methods are based on the genotype rather than the phenotype, and therefore the phylogenetically informative characters are not affected by environmental and physiological factors. Also, DNA samples can be prepared non-destructively from small pieces of plant tissues and subsequently PCR-amplified to yield sufficient amounts of template DNA for molecular genetic analysis. Thus DNA-based molecular markers have been extensively used for the study of genetic diversity and evolutionary relationships in plants, including medicinal herbs. However, an integrative approach, using both molecular marker techniques and modern chemical analytical tools, to the identification/discovery of medicinal plants is still rare. Through DNA polymorphism assay of selected genera/families that contain important medicinal plants, we may better understand their evolutionary relationships and gain insight into the distribution of certain medicinal active components in the genera or families and identify new sources of active components-containing medicinal plants through chemical assays. The main objectives of the research: o Comparative analysis of bioactive compounds in selected medicinal plants and their closest wild relatives. o Phylogenetic analysis of species relationships using molecular marker systems, including ISSR, AFLP and ITS at both population and species levels and more conservative DNA sequences at the genus/family levels; o Investigation of population structure, reproductive ecology and genetic diversity in the endangered medicinal species for developing conservation and management strategies for sustainable utilization of natural resources. |
| List of Research Outputs |
| Bao J., Corke H. and Sun M., Analysis of genetic diversity and relationships in waxy rice (Oryza sativa L.) using AFLP and ISSR markers, Genetic Resources and Crop Evolution. 2006, 53: 323-330. |
| Bao J., Shen S.Q., Sun M. and Corke H., Analysis of genotypic diversity in the starch physicochemical properties of nonwaxy rice: Apparent amylose content, pasting viscosity and gel texture, Starch. 2006, 58: 259-267. |
| Cai Y., Sun M. and Corke H., Development and application of betalains from the Amaranthaceae, Trends in food science & technology. 2005, 16: 370-376 (invited review). |
| Cai Y., Sun M. and Corke H., HPLC characterization of betalains from plants in the Amaranthaceae, Journal of Chromatographic Science. 2005, 43: 454-460. |
| Cai Y., Xing J., Sun M., Zhang Z.Q. and Corke H., Phenolic antioxidants (hydrolyzable tannins, flavonols, and anthocyanins) identified by LC-ESI-MS and MALDI-QIT-TOF MS from Rosa chinensis flowers, Journal of Agricultural and Food Chemistry. 2005, 53: 9940-9948. |
| Cai Y., Xing J., Sun M. and Corke H., Rapid identification of betacyanins from Amaranthus tricolor, Gomphrena globosa, and Hylocereus polyrhizus by matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight mass spectrometry (MALDI-QIT-TOF MS), Journal of Agricultural and Food Chemistry. 2006, 54 (18): 6520-6526. |
| Cai Y., Sun M., Xing J., Luo Q. and Corke H., Structure-radical scavenging activity relationships of phenolic compounds from traditional Chinese medicinal plants, Life Sciences. 2006, 78: 2872-2888. |
| Lu B., Baga M., Sun M., Corke H. and Chibbar R.N., Isolation, characterization, and expression of starch synthase genes from grain amaranth, The 12th Annual Life Sciences Research Conference, March 17 - 18, 2005, University of Saskastchewan, Canada. 2005. |
| Shan B., Cai Y., Sun M. and Corke H., Antioxidant capacity of 26 common spice extracts and characterization of their phenolic constituents, Journal of Agricultural and Food Chemistry. 2005, 53: 7749-7759. |
| Sun M., Natural hybridization in mangroves: molecular approach to hybrid identification, maternity confirmation and introgression, XVII International Botanical Congress, 17 - 23 July 2005, Vienna, Austria. 2005. |
| Researcher : Sze KL |
| List of Research Outputs |
| Lui W.Y., Sze K.L. and Lee W.W.M., Nectin-2 expression in testicular cells is controlled via the functional cooperation between transcription factors of the Sp1, CREB, and AP-1 families, Journal of Cellular Physiology. 2006, 207: 144-157. |
| Researcher : Tan-Un KC |
| Project Title: | Characterization of cytoglobin, a potential biomarker for the diagnosis of human liver fibrosis |
| Investigator(s): | Tan-Un KC, Ng IOL |
| Department: | Zoology |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 05/2005 |
| Completion Date: | 12/2006 |
| Abstract: |
| The objectives of this project are to 1) use cytoglobin as a biomarker for the diagnosis of human liver fibrosis; 2) identify the regulatory elements that control cytoglobin gene expression in human. |
| Project Title: | Quantitation of cytoglobin expression in liver of carbon tetrachloride treated mice |
| Investigator(s): | Tan-Un KC |
| Department: | Zoology |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 02/2006 |
| Abstract: |
| Cytoglobin (Cygb) is a protein first discovered in fibrotic rat livers (Kawada et al., 2001), and its function is currently unknown. It binds oxygen reversibly via its heme group (Sawai et al., 2003). It is expressed in the connective tissues of a wide variety of organs. We and others have demonstrated its localization in fibroblasts and in the hepatic stellate cells (HSCs) of the liver (Schmidt et al., 2004 and our unpublished data). As both types of cells are involved in the synthesis of the extra-cellular matrix (ECM), we hypothesize that cytoglobin may be related to the metabolism of ECM molecules. Upon liver injury, the functional phenotype of HSCs changes from a quiescent retinoid-storing one to a matrix-synthesizing one. During this trans-differentiation, the production of extra-cellular matrix molecules e.g. collages types I and III by HSCs, increases remarkably (Okazaki et al., 2003). Cygb had been shown to be time-dependently up-regulated during culture of primary HSCs, in the presence or absence of sera (Nakatani et al., 2004), but its expression pattern in fibrotic regions in vivo has not been described. The objective of this project is to demonstrate quantitatively the expression of cytoglobin in fibrotic lesions of the mouse liver in a time-dependent manner in relation to that of ECM molecules using laser capture microdissection (LCM). References 1. Isao Okazaki, Yosifumi Ninomiya, Tanikawa Kyuichi, Scott I. Friedman, Extracellular matrix and the liver: approach to gene therapy, Amsterdam: Academic Press, 2003. 2. Nakatani K, Okuyama H, Shimahara Y, Saeki S, Kim DH, Nakajima Y, Seki S, Kawada N, Yoshizato K Lab Invest. (2004) 84, 91-101. 3. Norifumi Kawada, Dan Bach Kristensen, Kinji Asahina, Kazuki Nakatani, Yukiko Minamiyama, Shuichi Seki, and Katsutoshi Yoshizato J. Biol. Chem. (2001), 276, 25318-25323. 4. Sawai H, Kawada N, Yoshizato K, Nakajima H, Aono S, Shiro Y. Biochemistry, (2003) 42, 5133-42. 5. Schmidt M, Gerlach F, Avivi A, Laufs T, Wystub S, Simpson JC, Nevo E, Saaler-Reinhardt S, Reuss S, Hankeln T, Burmester T. J Biol Chem. (2004) 279, 8063-9. |
| List of Research Outputs |
| Guo X., Philipsen S. and Tan-Un K.C., Characterization of human cytoglobin gene promoter region, Biochimica et Biophysica Acta. 2006, 1759: 208-215. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Retinoic acid-induced human secretin gene expression in neuronal cells is mediated by cyclin-dependent kinase 1, Annals New York Academy of Sciences. 2006, 1070: 393-398. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, 7th International Symposium on VIP, PACAP and Related Peptides, Rouen, France.. 2005. |
| Lee T.O., Tan-Un K.C. and Chow B.K.C., Transcriptional regulation of the human secretin gene in duodenal and neuronal cells, Regulatory Peptides. 2006, 130(3): 167. |
| Man K.N.M., Philipsen S. and Tan-Un K.C., Localization of cytoglobin, a novel protein, in lung tissues, European Respiratory Society Congress, September 2005, European Respiratory Journal. 2005, 26(Supp). 49, 551s. |
| Researcher : Tiu HK |
| List of Research Outputs |
| Tiu H.K., Hui J.H.L., He J.G., Tobe S.S. and Chan S.M., Characterization of Vitellogenin in the Shrimp Metapenaeus ensis: Expression Studies and Hormonal Regulation of MeVg1 Transcription In Vitro, Molecular Reproduction and Development. 2006, 73: 424-436. |
| Tiu H.K., Hui J.H.L., Mak A.S.C., He J.G. and Chan S.M., Equal contribution of hepatopancreas and ovary to the production of vitellogenin (PmVg1) transcripts in the tiger shrimp, Penaeus monodon, Aquaculture. 2006, 254: 666-674. |
| Researcher : Wan JMF |
| Project Title: | Effects of tumor necrosis factor-[alpha]TNF-[alpha] on cyclins and related cell cycle proteins expressions in human tumor cell lines as determined |
| Investigator(s): | Wan JMF |
| Department: | Zoology |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 07/1995 |
| Abstract: |
| To investigate: a) the effect of TNF on cyclins: D, E, A, B expression by flow cytometry; b) the effect of TNF on P21, PCNA expression by cytometric studies; c) the effect of TNF on tumor cell lines proliferation and apoptosis studied by flow cytometry. |
| Project Title: | The role of free radicals and antioxidants in motor neuron degenerative disease |
| Investigator(s): | Wan JMF, Vacca-Galloway LL |
| Department: | Zoology |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 07/1995 |
| Abstract: |
| There are increasing evidence indicating the involvement of free radicals damage in many chronic diseases such as Parkinson's disease, Alzheimer's disease and neurondegenerative disease. By using a motor neuron degenerative disease mouse model, to investigate the roles of free radicals in the disease process and investigating whether antioxidants such as vitamins E and C can be any therapeutic use by naturalizing the free radicals. |
| Project Title: | The effects of different dietary fatty acids on the development of mammary tumors in female fischer 344 rats |
| Investigator(s): | Wan JMF |
| Department: | Zoology |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 09/1995 |
| Abstract: |
| Exciting evidences demonstrated that the quality of dietary fatty acids, especially W-6 and W-3 polyunsaturated fatty acids affect the development of cancers such as the colon, breast, and prostate. This project aims to investigate the effect of saturated, monosaturated W-3, and W-6 polyunsaturated fatty acids on breast cancer cells proliferation by using flow cytometry technology. The data in this study will help us understand the mechanisms involved in more depth. |
| Project Title: | The effects of antioxidants on small cell lung cancer cell line, NCI-H446 |
| Investigator(s): | Wan JMF |
| Department: | Zoology |
| Source(s) of Funding: | Other Funding Scheme |
| Start Date: | 09/1995 |
| Abstract: |
| Lung cancer is almost certainly the most common cancer in the world today. Over the past several years, work has focused on characterizing the prevention, inhibition and regression of lung cancer by [beta]-carotene, vitamin C and vitamin C which act as antioxidants. This study aims to investigate the antiproliferative potential of [beta]-carotene and retinoids by flow cytometry technology. The data will help us to understand how antioxidants prevent lung cancer formation and progression. |
| Project Title: | To determine the mechanisms of apoptosis of polysaccharide peptide (PSP) in human leukemic cell lines and human normal lymphocytes |
| Investigator(s): | Wan JMF |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2003 |
| Abstract: |
| To determine the apoptotic pathways of PSP-induced apoptosis in various human leukemic cell eines; to identify the survival pathways in the human normal lymphocytes in response to PSP-induced apoptosis. |
| Project Title: | To determine the mechanisms of apoptosis of polysaccharide peptide (PSP) in human leukemic cell lines and human normal lymphocytes |
| Investigator(s): | Wan JMF |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 09/2003 |
| Abstract: |
| N/A |
| Project Title: | Molecular Structural Determination of Protein-bound Polysaccharide peptide (PSP) isolated from the Chinese Medicinal Mushroom Coriolus versicolor (Cov-1) |
| Investigator(s): | Wan JMF, Sze KH, Che CM |
| Department: | Zoology |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 05/2006 |
| Abstract: |
| Purpose: The goal of this project is to determine the molecular structure of Cov-1 PSP molecule dervies from the Chinese Medicine mushroom Yun Zhi. Key Issues: Polysaccharide peptide (PSP) isolated from the mycelia of fungus Coriolus versicolor (Cov-1 strain) or Yun Zhi is Chinese Medicine best known for its anticancer and immunomodulatory properties. PSP is classified as a biological response modifier (Ng TB 1998 review) with the ability to induce gamma-interferon, intelerukin-2 production, T-cell proliferation in cancer patients. A small peptide with a molecular weight of 16-18 kDa originating from PSP has been produced with antiproliferative and antitumor activities (Yang et al 1992). We have recently published the cellular and molecular detailed cell death induction pathways of PSP on human leukemic cells by flow cytometry (Yang &Wan 2005, Hui and Wan 2005) and cDNA arrays (Zeng, Leung &Wan 2005). The ability of the Cov-1 PSP to distinguish cancerous cells from non-cancerous cells as recently determined by us (Yang & Wan 2005) and previously by others (NT 1998 review), indeed suggesting its uniqueness potential in its development into anticancer agent. Cov-1 PSP possesses a molecular weight of approximately 100 kDa. The polysaccharide moiety is a heteropolysaccharide made up of monosccharides with alpha-1, 4 and beta-1, 3 glucosidic linkages consisting of glucose, glactose, mannose, xylose, arabinose and trace amount of rhamnose (NT 1998 review). The polypeptide unit contains glutamic and aspartic acids as the abundant amino acids. PSP is presently used as over-the-counter dietary health supplement with multiple health claims such as anti-cancer, anti-inflammatory, antioxidant, anti-allergic and anti-viral. Our preliminary work on separation and purification of PSP by HPLC technology has identified two factions: a small molecular fraction of < 5000 Da and a macro-molecular fraction of > 5000 Da. The former fraction exhibited anticancer effect onhuman leukemia and the latter fraction exhibited immunomodulatory effect on healthy normal human T-lymphocytes. Despite the promising potentials of PSP, pharmaceumatical industry is not willing to invest into its therapeutic development unless the molecular structural information is apparent. It is urgent to identify the molecular structure of the PSP molecules as soon as possible since this unqiue Cov-1 strain medicinal mushroom exhibits most promising anticancer and immunomodulatory properties. The Cov-1 PSP strain is currently in Phase III clinical testing for anti-cancer properties in China with sucessful outcomes. Up-to-now, there are no clear structural determination of the PSP parent molecule and its fractions. The present propoal thus sought to reveal the molecular structure of PSP. Issues to be addressed: Additional experiments are also required to more thoroughly assess the molecular structure of the compounds that are responsible for the immunomodulatory effect and that which posses anticancer activities. Purpose of this proposed project: The goal of this project is to determine the structure of Cov-1 PSP molecule by carrying out the following objectives: 1. to purify fractions of PSP by DEAE Anion-exchange HPLC system 2. to elucidate the molecular structure of PSP by spectroscpic analysis, NMR and/or X-ray crystallograpghy. We believe that the structural determination of the molecular structure of PSP is urgently needed as to provide important insight into its distinct functions/roles in treatment of cancer and infectious diseases. References Cited: Yang MM et al (1992). The anticancer effect of a small polypeptide from Coriolus versicolor. Am J Clin Med 20: 221-32. Ng TB (1998). A review or research on the protein-bound polysaccharide PSP from the mushroom Coriolus versiolor. Gen Pharmacol 30: 1-4. Zeng F, Leung F and Wan JMF (2005). Molecular characterization of Coriolus versicolor in human promyelotic leukemic HL-60 cells using cDNA microarray. In J of Oncology 26: 10-16. Hui K.P.Y& Wan JMF (2005) Induction of S phase cells arrest and caspase activation by polysaccharide peptide (PSP) isolated from coriolus versicolor enhanced the cell-cycle-dependent activity and apoptotic cell death of Doxorubicin and Etoposide but not Cytarabine in HL-60 cells. Oncology Reports. 14(1): 145-165. Yang X and Wan JMF (2005). The Cell Death Process of the Anticancer Agent Polysaccharide-peptide (PSP) in Human Promyelocytic Leukemic HL-60 Cells. Oncology Reports 13(6): 1201-1221. |
| List of Research Outputs |
| Chan L.L., Sit W.H., Lam P.K.S., Hsieh D.P.H.T., Hodgkiss I.J., Wan J.M.F., Ho A.Y.T., Choi N.M.C., Wang D.Z. and Dudgeon D., Identification and characterization of a "biomarker of toxicity" from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae), Proteomics. 2006, 6: 654-666. |
| Kumar D.S.S., Lau W.C.S., Wan J.M.F., Yang D. and Hyde K.D., Immunomodulatory compounds from Pestalotiopsis leucothës, an endophytic fungus from Tripterygium wilfordii, Life Sciences. 2005, 78: 147-156. |
| Lee C.L., Sit W.H. and Wan J.M.F., Cyclosporine A-like Immunomodulatory Benefits of Polysaccharopeptide (PSP) Derived from Coriolus Versicolor in Activated Human T-cells, Inflammation research 54(supp. 2):S130, 7th World Congress of Inflammation, Australia. 2005. |
| Lee C.L., Yang X. and Wan J.M.F., The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor, Enzyme and Microbial Technology. 2006, 38: 14-21. |
| Liu S.Y.K., Tsang J.S.H., Leung P.C., Chow B.K.C. and Wan J.M.F., Proteomics of Macrophage; an Approach to Understand Lipopolysaccharide-challenged inflammation, Molecular & Cellular Proteomics 4.8:S231, HUPO 4th Annual World Congress, August 28-September 1, 2005, Munich. 2005. |
| Ngai H.Y.H., Sit W.H., Jiang P., Thongboonkerd V. and Wan J.M.F., Comparative Proteomics Analysis Reveals Haptoglobin as a Possible Novel Marker in Human Membranous Nephropathy, Molecular ＆ Cellular Proteomics, Human Proteome Organisation, 5th Annual World Congress, October 28 - November 1, 2006, Long Beach, California. 2006, 5.10: 496. |
| Wan J.M.F., Hui P.Y. and Sit W.H., Induction of S phase cell arrest and caspase activation by polysaccharide peptide (PSP) isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cells, 10th World Congress on Advances in Oncology and 8th International Symposium on Molecular Medicine, 2005 Greece. 2005. |
| Wan J.M.F., Nutrigenomics: Exploiting system biology in Chinese nutrition and medicine, Nutrigenomics and Health. From Vision to Food. LMC International Food Congress 2006, Denmark March 15-16 Scandinavian Journal of Food and Nutrition Supplement 1, volume 50, p16-17, 2006. |
| Wan J.M.F., Sit W.H., Lee C.L., Fu H.M.K. and Chan D.K.O., Protection of lethal toxicity of endotoxin by Salvia miltiorrhiza BUNGE is via reduction in tumor necrosis factor alpha release and liver injury, International Immunopharmacology. 2006, 6: 750-758. |
| Researcher : Wang L |
| List of Research Outputs |
| Cheng T.H.T., Cheung H.K., Wang L., Wang T. and Xu R.J., Effects of green tea extracts on learning and memory behaviour in rats, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S89. |
| Wang L., Wong A.O.L. and Xu R.J., Effects of prenatal malnutrition on hippocampal BDNF expression and spatial learning behavior in rats, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S78. |
| Wang L., Chen Z.Y. and Xu R.J., The effect of dietary n-3 fatty acid deficiency on BDNF expression and spatial learning behavior in rats, Experimental Biology 2006 San Francisco, California, April 1 - April 5, 2006, p. A1002, (Abstract No. 648.5). 2006. |
| Wang L. and Xu R.J., The effect of postnatal protein malnutrition on spatial learning and memory behavior in rats, Experimental Biology 2006, San Francisco, California, April 1 - April 5, 2006, p.176, (Abstract No. 134.2). 2006. |
| Researcher : Wang Y |
| List of Research Outputs |
| Li J., Wang Y. and Leung F.C.C., Cloning of Chicken Pre-B-cell Colony-Enhancing Factor 1 (PBEF1) and Characterization of its Spatial Expression, Poultry Science 84(Supp). 2005, 74. |
| Li J., Wang Y., Hon C.C. and Leung F.C.C., Cloning of Smad 1, Smad5 and Smad8 from the Chicken Ovary and Characterization of Their Expression during Chicken Ovarian Development, Poultry Science 84(Supp). 2005, 74. |
| Wang C.Y., Wang Y. and Leung F.C.C., Expression Profiles of Prolactin Receptors during Chicken Embryonic Development, Poultry Science 84(Supp). 2005, 44. |
| Wang C.Y., Wang Y., Li J. and Leung F.C.C., Expression Profiles of the Growth Hormone-releasing Hormone (GHRH) and Growth Hormone-releasing Hormone Receptor (GHRHR) during Chicken Embryonic Pituitary Development, Poultry Science. 2006, 85: 569-576. |
| Wang Y., Wang Z. and Leung F.C.C., A chicken full-length cDNAs database, 2nd International Symposium on Animal Functional Genomics, May 16-19, 2006. |
| Wang Y. and Leung F.C.C., Long inverted repeats in eukaryotic genomes: Recombinogenic motifs determine genomic plasticity, Federation of European Biochemical Society letters. 2006, 580: 1277-1284. |
| Wang Y., Li J. and Leung F.C.C., Regulation of chicken Heparin-binding EGF-like Growth Factor (HB-EGF) Expression by EGF and TGF-a in the cultured ovarian granulosa cells, Poultry Science 84(Supp). 2005, 44. |
| Wang Z., Wang Y. and Leung F.C.C., A database of chicken ESTs alignments with human and mouse full length cDNAs, Proceeding Plant and Animal Genome XIV, San Diego, CA. 2006, p.242. |
| Researcher : Wang Z |
| List of Research Outputs |
| Wang Y., Wang Z. and Leung F.C.C., A chicken full-length cDNAs database, 2nd International Symposium on Animal Functional Genomics, May 16-19, 2006. |
| Wang Z., Wang Y. and Leung F.C.C., A database of chicken ESTs alignments with human and mouse full length cDNAs, Proceeding Plant and Animal Genome XIV, San Diego, CA. 2006, p.242. |
| Researcher : Wong AOL |
| Project Title: | Regulation of growth hormone synthesis in fish: mechanisms of action of the novel hypophysiotropic factor pituitary adenylate cyclase-activating polypeptide |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 12/2002 |
| Completion Date: | 12/2005 |
| Abstract: |
| The project aims at understanding the mechanisms and evolution of neuroendocrine control of body growth in lower vertebrates. The research on GH synthesis and secretion of vertebrate endocrine will also provide new insights on the role of hormone biosynthesis in "stimulus-secretion" coupling of vertebrate endocrine systems. |
| Project Title: | Novel aspects of growth hormone autoregulation in grass carp pituitary cells:- intrapituitary "ultra-short" feedback on growth hormone gene expression in fish? |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 12/2003 |
| Abstract: |
| To establish the structural identity of grass carp GH receptor; to provide a full-length receptor cDNA for subsequent functional expression studies; to confirm the expression of GH receptors in immuno-identified grass carp somatotrophs; to characterize ligand-binding properties of grass carp GH receptors expressed in CHO cells; to show the functional coupling of grass carp GH receptors with Ca2+i and Spi 2.1 gene activation; to examine the role of GH on GH synthesis and GH mRNA expression in grass carp pituitary cells; to examine the role of endogenous GH on GH mRNA expression induced by GH-releasing factors; to examine the effects of inhibiting the JAK/MARK cascade in GH-induced GH mRNA expression; to examine the effects of inhibiting the JAK/IRS/P13K cascade in GH-induced GH mRNA expression; to demonstrate GH modulation of GH transcript stability by altering the half-life (T1/2) of GHmRNA; to show that GH is essential to maintain basal expression of GH primary transcripts in the nucleus; to test if GH can alter the 5' promoter activity of grass carp GH gene using a reporter gene assay. |
| Project Title: | Novel aspects of growth hormone autoregulation in grass carp pituitary cells:- intrapituitary "ultra-short" feedback on growth hormone gene expression in fish? |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 12/2003 |
| Abstract: |
| N/A |
| Project Title: | Intrapituitary feedback loop regulating growth hormone synthesis:- paracrine regulation of growth hormone gene expression by gonadotropin release in grass carp pituitary cells |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Abstract: |
| N/A |
| Project Title: | Intrapituitary feedback loop regulating growth hormone synthesis:- paracrine regulation of growth hormone gene expression by gonadotropin release in grass carp pituitary cells |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 01/2005 |
| Abstract: |
| To study: (1) molecular cloning of grass carp GTH-II receptor by 3' and 5' RACE; (2) tissue distribution of GTH-II receptor transcripts in Chinese grass carp; (3) functional expression and characterization of grass carp GTH-II receptors; (4) GH mRNA expression regulated by interactions between GTH-II and GH regulators; (5) post-receptor signaling mechanisms mediating GTH-induced GH gene expression in grass carp pituitary cells; (6) functional role of GH mRNA stability and GH primary transcript in GTH-induced GH gene expression in grass carp pituitary cells; modulation of the 5' promoter activity of grass carp GH gene by GTH-II through the AC/cAMP/PKA/CREB pathway. |
| Project Title: | Grass Carp CREB: Molecular Cloning, Functional Characterization, and Regulation of Transcript Expression at the Pituitary Level |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2006 |
| Completion Date: | 12/2006 |
| Abstract: |
| (I) Background (see figures attached) : Growth hormone (GH) is an important pituitary hormone regulating body growth and metabolism in vertebrates. In mammals, GH release is under the dual control of stimulatory GH-releasing hormone (GHRH) and inhibitory somatostatin (SRIF). These two hypothalamic factors by working together determine the pulsatile pattern of GH release in vivo. Although GHRH is known to induce GH release & synthesis in somatotrophs, the inhibitory action of SRIF is largely restricted to GH secretion. Apparently, SRIF has no effects on GH production nor GH gene expression at the pituitary cell level. Nevertheless, SRIF inhibition of GH mRNA levels has been reported in cancer cells (e.g. pituitary adenomas), suggesting that SRIF may also inhibit GH synthesis under pathological conditions. CREB is a ubiquitous transcription factor known to transactivate target genes via the CRE site(s) present in the target gene promoters. Although CREB is involved in GHRH-induced GH gene expression, its functional coupling with SRIF in GH regulation has not been previously examined. Recently, as a part of our on-going research to study GH gene regulation in fish, we have cloned a partial cDNA fragment for grass carp CREB. The fragment contains the DNA sequence encoding the C-terminal bZip region for DNA binding and the deduced a.a. sequence is highly homologous to the corresponding region of human CREB [Fig.1A]. Protein modeling reveals that the 3-D structure and surface biochemical properties of the carp CREB bZip domain is very similar if not identical to the human counterpart [Fig.1B]. In parallel studies in GH3 cells transfected with a reporter construct carrying a grass carp GH promoter, over-expression of rat CREB was found to inhibit GH promoter activity [Fig.2A] and the “CREB responsive sequence” was mapped to the 5’ promoter region from -742 to -656 [Fig.2B]. These results have demonstrated for the first time that CREB can exert a negative effect on GH gene expression in fish model. Using the partial CREB cDNA as a probe, we have also found that (1) SRIF inhibits GH mRNA expression with a concurrent rise in CREB transcripts in grass carp pituitary cells [Fig.3A,B], (2) CREB gene expression induced by SRIF occurs in carp somatotrophs [Fig.3C] with parallel increases in CREB protein expression & protein phosphorylation [Fig.3D], and (3) SRIF-induced CREB mRNA expression at the pituitary level can be blocked by the cAMP analogs, Ca2+ ionophores, and inhibitors for PLC & PKC [Fig.4A,B]. These results, as a whole, have prompted us to speculate that CREB may serve as a novel mediator for SRIF inhibition of GH gene expression in the carp species. (II) Objective of the proposal : In this study, we propose to clone the full-length cDNA for grass carp CREB and examine its functional role in SRIF inhibition of GH gene expression at the pituitary cell level. We will test the hypothesis that SRIF stimulates CREB gene expression in carp somatotrophs by differential coupling to cAMP/PKA, Ca2+/calmodulin and PLC/PKC cascades, which then down-regulates GH gene transcription by acting on cis-acting element(s) in the GH promoter. The specific aims to be addressed can be summarized as follows: -· 1) To establish the structural identity of grass carp CREB by molecular cloning 2) To confirm the transactivation activity of carp CREB in CRE-containing gene promoter by functional expression 3) To study the role of cAMP/PKA, Ca2+/CaM, & PLC/PKC cascades in SRIF induction of CREB gene expression 4) To map the location of responsive elements in GH promoter for CREB inhibition of GH gene transcription |
| Project Title: | Modulation of calmodulin gene expression as a novel mechanism for growth hormone feedback control by insulin-like growth factor in grass carp pituitary cells |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2006 |
| Abstract: |
| N/A |
| Project Title: | Modulation of calmodulin gene expression as a novel mechanism for growth hormone feedback control by insulin-like growth factor in grass carp pituitary cells |
| Investigator(s): | Wong AOL |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 01/2006 |
| Abstract: |
| (1) Molecular cloning and functional studies of calmodulin in fish models; (2) IGF regulation of calmodulin (CaM) gene expression in grass carp pituitary cells; (3) functional role of CaM in IGF feedback control on growth hormone (GH) gene expression; (4) signal transduction for IGF regulation of CaM gene expression at the pituitary level; (5) molecular cloning and functional studies of CREB in grass carp pituitary cells; (6) Ca2+/CaM-dependent pathways mediate GH feedback control by IGF via modulation of CREB gene expression. |
| List of Research Outputs |
| Wang L., Wong A.O.L. and Xu R.J., Effects of prenatal malnutrition on hippocampal BDNF expression and spatial learning behavior in rats, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S78. |
| Wong A.O.L., Zhou H., Jiang Y. and Ko K.W., Feedback regulation of growth hormone synthesis and secretion in fish and emerging concept of intrapituitary feedback loop, Comparative Biochemistry of Physiology . 2006, (Part A) 144: 284-305. |
| Wong A.O.L., Chuk M.C.Y., Chan H.C. and Lee K.Y., Mechanisms for gonadotropin-releasing hormone potentiation of growth hormone rebound following norepinephrine inhibition in goldfish pituitary cells, American Journal of Physiology. 2006. |
| Wong A.O.L., Li W., Leung C.Y., Huo L. and Zhou H., Pituitary adenylate cyclase-activating polypeptide (PACAP) as a growth hormone (GH)-releasing factor in grass carp: I. Functional coupling of cAMP- and Ca2+/ calmodulin-dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells. , Endocrinology. USA, The Endocrine Society, 2005, 146: 5407-5424. |
| Wong A.O.L., Zhou H., Ko K.W. and Ho K.K., 在草魚中新發現的生長激素合成和分泌的反饙調控機理, 中國科技發展精典文庫,第一部分 農業科技, 第七章,漁業科技, 2006, pp.1-7. |
| Researcher : Wong AST |
| Project Title: | The role of GRP/BiP and MnSOD in BRCA1-mediated breast/ovarian tumor suppression |
| Investigator(s): | Wong AST, He Q |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2004 |
| Abstract: |
| To elucidate the role of GRP78/BiP and MnSOD in BRCA1-mediated breast and ovarian tumor suppression; to define the direct effect of BRCA1 on the altered expressions of GRP78/BiP and MnSOD by transfection and expression of functional BRCA1 gene in OSE cells; to examine the role of GRP78/BiP and MnSOD overespression in the regulation of apoptosis, cell growth and cell cycle progression, and in protecting cells from external stresses and resistance to cytotoxic drugs in breast and ovarian epithelial cells; to determine the mechanism (signal pathway) by which BRCA1 alters the gene activities of GRP78/BiP and MnSOD. |
| Project Title: | Synergistic action of epidermal growth factor and Met signalling pathways in the motile and invasive phenotype of ovarian cancer |
| Investigator(s): | Wong AST |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Completion Date: | 10/2005 |
| Abstract: |
| To study the downstream signalling pathways mediated by the cross-talk between EGF and HGF for the invasive growth in human ovarian tumours. |
| Project Title: | The role of GRP/BiP and MnSOD in BRCA1-mediated breast/ovarian tumor suppression |
| Investigator(s): | Wong AST |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Abstract: |
| N/A |
| Project Title: | p70S6K in human ovarian cancer |
| Investigator(s): | Wong AST |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2006 |
| Abstract: |
| N/A |
| Project Title: | p70S6K in human ovarian cancer |
| Investigator(s): | Wong AST |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 01/2006 |
| Abstract: |
| To determine whether p70S6K plays a specific and direct role in actin reorganization, tumor cell migration and invasion; to determine the mechanism underlying its regulation of the actin cytoskeleton. |
| List of Research Outputs |
| Cheung W.T., Au S.C.L., Cheung A.N.Y., Ngan H.Y.S. and Wong A.S.T., Pigment Epithelium-Derived Factor, a Gene Down-Regulated by Estrogen, Mediates Growth Inhibition in Human Ovarian Surface Epithelial and Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-32). 2006. |
| Cheung W.T., Au S.C., Cheung A.N.Y., Ngan H.Y.S., Tombran-Tink J., Auersperg N. and Wong A.S.T., Pigment epithelium-derived factor is estrogen sensitive and inhibits the growth of human ovarian cancer and ovarian surface epithelial cells, Endocrinology. 2006, 147(9): 4179-91. |
| Pon Y.L., Auersperg N. and Wong A.S.T., Gonadotropins induce loss of N-cadherin, increased transcriptional activity of b-catenin, and apoptosis in human ovarian surface epithelial cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.482 (Abstract No. P2-345). 2006. |
| Wong A.S.T., Adhesion, signaling, transcription in human ovarian surface epithelium, The International Ovarian Conference, Yokohama, Japan, May . 2006. |
| Wong A.S.T., Control of ovarian surface epithelial cell survival and apoptosis by N-cadherin, Northwestern University, Chicago, IL., June . 2006. |
| Wong A.S.T., Lee A.S., Wong K.Y., Kwan W.Y. and Wong E.S.Y., Deregulation of the Stress-Inducible GRP78 in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.732 (Abstract No. P3-377). 2006. |
| Wong A.S.T., National University of Singapore Academic Research Grant (ad hoc reviewer), 2005. |
| Zhou H. and Wong A.S.T., Activation of p70S6K induces expression of matrix metalloproteinase 9 associated with hepatocyte growth factor-mediated invasion in human ovarian cancer cells, Endocrinology. 2006, 147: 2557-2566. |
| Zhou H. and Wong A.S.T., Up-Regulation of Matrix Metalloproteinase-9 Is Associated with Hepatocyte Growth Factor-Mediated Invasion in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-30). 2006. |
| Researcher : Wong CH |
| List of Research Outputs |
| Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases, Journal of Biological Chemistry. 2005, 280(7): 25029-25047. |
| Xia W., Wong C.H., Lee P.Y., Lee W.W.M. and Cheng C.Y., Disruption Of Sertoli-germ Cell Adhesion Function In The Seminiferous Epithelium Of The Rat Testis Can Be Limited To Adherens Junctions Without Affecting The Blood-testis Barrier Integrity: An In Vivo Study Using An Androgen Suppression Model. , Journal of Cellular Physiology. 2005, 205: 141-157. |
| Researcher : Wong ESY |
| List of Research Outputs |
| Wong A.S.T., Lee A.S., Wong K.Y., Kwan W.Y. and Wong E.S.Y., Deregulation of the Stress-Inducible GRP78 in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.732 (Abstract No. P3-377). 2006. |
| Researcher : Wong KWC |
| List of Research Outputs |
| Wong K.W.C., Li Y.Y. and Leung F.C.C., Quantification of porcine cytokine mRNA by real-time RT-PCR, Proceedings of the 86th Annual Conference of Research Workers in Animal Diseases. St. Louis, Missouri . 2005, p.101. |
| Researcher : Wong KY |
| List of Research Outputs |
| Wong A.S.T., Lee A.S., Wong K.Y., Kwan W.Y. and Wong E.S.Y., Deregulation of the Stress-Inducible GRP78 in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.732 (Abstract No. P3-377). 2006. |
| Researcher : Wong TY |
| List of Research Outputs |
| Wong T.Y., Hon C.C. and Leung F.C.C., Molecular characterization of IBDV-induced in vitro apoptosis using cDNA microarrays, Proceedings of the Third Symposium on Genetics of Animal of Health, Ames, IA. 2005, p.130. |
| Researcher : Wu WK |
| List of Research Outputs |
| Wu W.K., Mak C. and Ko R.C.C., Cloning and characterization of the Cu/Zn superoxide dismutase of Trichinella pseudospiralis, Parasitology Research. 2005, 98: 281-287. |
| Researcher : Xia W |
| List of Research Outputs |
| Xia W., Mruk D.D., Lee W.W.M. and Cheng C.Y., Cytokines And Junction Restructuring During Spermatogenesis – A Lesson To Learn From The Testis. , Cytokine & Growth Factor Reviews . 2005, 16: 469-493. |
| Xia W., Mruk D.D., Lee W.W.M. and Cheng C.Y., Differential Interactions between Transforming Growth Factor-b3/TbR1, TAB1, and CD2AP Disrupt Blood-Testis Barrier and Sertoli-Germ Cell Adhesion, Journal of Biological Chemistry. 2006, 281, No. 24: p.16799-16813. |
| Xia W., Wong C.H., Lee P.Y., Lee W.W.M. and Cheng C.Y., Disruption Of Sertoli-germ Cell Adhesion Function In The Seminiferous Epithelium Of The Rat Testis Can Be Limited To Adherens Junctions Without Affecting The Blood-testis Barrier Integrity: An In Vivo Study Using An Androgen Suppression Model. , Journal of Cellular Physiology. 2005, 205: 141-157. |
| Researcher : Xu RJ |
| Project Title: | A study on the physiological role of transforming growth factor [beta] in postnatal adaptation of the gastrointestinal tract in neonatal pigs |
| Investigator(s): | Xu RJ |
| Department: | Zoology |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To investigate the possible physiological role of TGF-[beta] in regulation of postnatal adaptation of the gastrointestinal tract in neonatal animals. |
| Project Title: | Postnatal adaptation of the gastrointestinal tract in neonatal pigs: a possible role of transforming growth factor-[beta] |
| Investigator(s): | Xu RJ |
| Department: | Zoology |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 12/2003 |
| Abstract: |
| To examine concentration changes of TGF-[beta] in milk during the lactation; to study chemical properties of TGF-[beta] in milk; to examine the stability of milk-borne TGF-[beta] in the GI lumen; to study the development changes of endogenous production of TGF-[beta] in the GI tract; to study the effects of milk on intestinal mutation in newborn piglets; to study the endogenous production of TGF-D and its receptors in the gastrointestinal tract in early-weaned piglets. |
| Project Title: | Postnatal adaptation of the gastrointestinal tract in neonatal pigs: a possible role of transforming growth factor-[beta] |
| Investigator(s): | Xu RJ |
| Department: | Zoology |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 12/2003 |
| Abstract: |
| N/A |
| List of Research Outputs |
| Cheng T.H.T., Cheung H.K., Wang L., Wang T. and Xu R.J., Effects of green tea extracts on learning and memory behaviour in rats, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S89. |
| Huo Y.J., Wang T., Xu R.J., Macdonald S., Liu G. and Shi F.X., Dietary Insulin Affects Leucine Aminopeptidase, and Growth Hormone, Insulin-Like Growth Factor I and Insulin Receptors in the Intestinal Mucosa of Neonatal Pigs, Biology of the Neonate. 2005, 893: 265-273. |
| Mei J., Zhang Y.Q., Wang T. and Xu R.J., Effects of oral ingestion of colostrum on intestinal expression of TGF-beta receptors in the newborn pig, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S89. |
| Pan M. and Xu R.J., Effects of gonadic hormone and phytoestrogen on fetal rat hippocampal neuron proliferation in vitro, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 Nov - 3 Dec 2005. 14 (Suppl) S61. |
| Wang L., Wong A.O.L. and Xu R.J., Effects of prenatal malnutrition on hippocampal BDNF expression and spatial learning behavior in rats, Asia Pacific Journal of Clinical Nutrition, Proceedings of the Nutrition Society of Australia (2005), Volume 29, Twenty-ninth Annual Scientific Meeting, Melbourne, Victoria, 30 November - 3 December 2005. 14 (Suppl) S78. |
| Wang L., Chen Z.Y. and Xu R.J., The effect of dietary n-3 fatty acid deficiency on BDNF expression and spatial learning behavior in rats, Experimental Biology 2006 San Francisco, California, April 1 - April 5, 2006, p. A1002, (Abstract No. 648.5). 2006. |
| Wang L. and Xu R.J., The effect of postnatal protein malnutrition on spatial learning and memory behavior in rats, Experimental Biology 2006, San Francisco, California, April 1 - April 5, 2006, p.176, (Abstract No. 134.2). 2006. |
| Researcher : Xue C |
| List of Research Outputs |
| Yip C.W., Xue C. and Leung F.C.C., Identification of Infectious Bursal Disease Virus binding proteins using virus overlay protein binding assays and mass spectrometry, Proceedings of the Third Symposium on Genetics of Animal of Health, Ames, IA. 2005, p.130. |
| Researcher : Yan HNH |
| List of Research Outputs |
| Yan H.N.H., Cheng C.Y. and Lee W.W.M., Laminin-333/a6b1 Integrin is a non-basement membrane cell adhesion protein complex at the ectoplasmic specialization of the rat testis, The 45th American Society for Cell Biology Annual Meeting, Dec 10-14 2005, San Francisco, CA, USA. 2005. |
| Researcher : Yang X |
| List of Research Outputs |
| Lee C.L., Yang X. and Wan J.M.F., The culture duration affects the immunomodulatory and anticancer effect of polysaccharopeptide derived from Coriolus versicolor, Enzyme and Microbial Technology. 2006, 38: 14-21. |
| Researcher : Yeung YS |
| List of Research Outputs |
| Chow Y.C.K., Yeung Y.S., Hon C.C., Zeng F., Law K.M. and Leung F.C.C., Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells, Federation of European Biochemical Society letters. 2005, 579: 6699-6704. |
| Researcher : Yip CW |
| List of Research Outputs |
| Hon C.C., Lam T.Y., Drummond A., Rambaut A., Lee Y.F., Yip C.W., Zeng F., Lam P.Y., Ng P.T.W. and Leung F.C.C., Phylogenetic analysis reveals coincidence of the expansion of very virulent Infectious Bursal Disease Virus and the reassortment of its genome segment B, Journal of Virology. 2006, 80: 8503-8509. |
| Yip C.W., Xue C. and Leung F.C.C., Identification of Infectious Bursal Disease Virus binding proteins using virus overlay protein binding assays and mass spectrometry, Proceedings of the Third Symposium on Genetics of Animal of Health, Ames, IA. 2005, p.130. |
| Researcher : Yuan Y |
| List of Research Outputs |
| Lee T.O., Lee H.Y.V., Yuan Y. and Chow B.K.C., Identification of repressor element 1 in secretin/PACAP/VIP genes, Annals New York Academy of Sciences. 2006, 1070: 388-392. |
| Researcher : Zeng F |
| List of Research Outputs |
| Chow Y.C.K., Yeung Y.S., Hon C.C., Zeng F., Law K.M. and Leung F.C.C., Adenovirus-mediated expression of the C-terminal domain of SARS-CoV spike protein is sufficient to induce apoptosis in Vero E6 cells, Federation of European Biochemical Society letters. 2005, 579: 6699-6704. |
| Hon C.C., Lam T.Y., Drummond A., Rambaut A., Lee Y.F., Yip C.W., Zeng F., Lam P.Y., Ng P.T.W. and Leung F.C.C., Phylogenetic analysis reveals coincidence of the expansion of very virulent Infectious Bursal Disease Virus and the reassortment of its genome segment B, Journal of Virology. 2006, 80: 8503-8509. |
| Researcher : Zhou H |
| List of Research Outputs |
| Wong A.O.L., Zhou H., Jiang Y. and Ko K.W., Feedback regulation of growth hormone synthesis and secretion in fish and emerging concept of intrapituitary feedback loop, Comparative Biochemistry of Physiology . 2006, (Part A) 144: 284-305. |
| Wong A.O.L., Li W., Leung C.Y., Huo L. and Zhou H., Pituitary adenylate cyclase-activating polypeptide (PACAP) as a growth hormone (GH)-releasing factor in grass carp: I. Functional coupling of cAMP- and Ca2+/ calmodulin-dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells. , Endocrinology. USA, The Endocrine Society, 2005, 146: 5407-5424. |
| Wong A.O.L., Zhou H., Ko K.W. and Ho K.K., 在草魚中新發現的生長激素合成和分泌的反饙調控機理, 中國科技發展精典文庫,第一部分 農業科技, 第七章,漁業科技, 2006, pp.1-7. |
| Zhou H. and Wong A.S.T., Activation of p70S6K induces expression of matrix metalloproteinase 9 associated with hepatocyte growth factor-mediated invasion in human ovarian cancer cells, Endocrinology. 2006, 147: 2557-2566. |
| Zhou H. and Wong A.S.T., Up-Regulation of Matrix Metalloproteinase-9 Is Associated with Hepatocyte Growth Factor-Mediated Invasion in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-30). 2006. |
| Researcher : Zhou H |
| List of Research Outputs |
| Wong A.O.L., Zhou H., Jiang Y. and Ko K.W., Feedback regulation of growth hormone synthesis and secretion in fish and emerging concept of intrapituitary feedback loop, Comparative Biochemistry of Physiology . 2006, (Part A) 144: 284-305. |
| Wong A.O.L., Li W., Leung C.Y., Huo L. and Zhou H., Pituitary adenylate cyclase-activating polypeptide (PACAP) as a growth hormone (GH)-releasing factor in grass carp: I. Functional coupling of cAMP- and Ca2+/ calmodulin-dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells. , Endocrinology. USA, The Endocrine Society, 2005, 146: 5407-5424. |
| Wong A.O.L., Zhou H., Ko K.W. and Ho K.K., 在草魚中新發現的生長激素合成和分泌的反饙調控機理, 中國科技發展精典文庫,第一部分 農業科技, 第七章,漁業科技, 2006, pp.1-7. |
| Zhou H. and Wong A.S.T., Activation of p70S6K induces expression of matrix metalloproteinase 9 associated with hepatocyte growth factor-mediated invasion in human ovarian cancer cells, Endocrinology. 2006, 147: 2557-2566. |
| Zhou H. and Wong A.S.T., Up-Regulation of Matrix Metalloproteinase-9 Is Associated with Hepatocyte Growth Factor-Mediated Invasion in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-30). 2006. |
| Researcher : Zhou H |
| List of Research Outputs |
| Wong A.O.L., Zhou H., Jiang Y. and Ko K.W., Feedback regulation of growth hormone synthesis and secretion in fish and emerging concept of intrapituitary feedback loop, Comparative Biochemistry of Physiology . 2006, (Part A) 144: 284-305. |
| Wong A.O.L., Li W., Leung C.Y., Huo L. and Zhou H., Pituitary adenylate cyclase-activating polypeptide (PACAP) as a growth hormone (GH)-releasing factor in grass carp: I. Functional coupling of cAMP- and Ca2+/ calmodulin-dependent signaling pathways in PACAP-induced GH secretion and GH gene expression in grass carp pituitary cells. , Endocrinology. USA, The Endocrine Society, 2005, 146: 5407-5424. |
| Wong A.O.L., Zhou H., Ko K.W. and Ho K.K., 在草魚中新發現的生長激素合成和分泌的反饙調控機理, 中國科技發展精典文庫,第一部分 農業科技, 第七章,漁業科技, 2006, pp.1-7. |
| Zhou H. and Wong A.S.T., Activation of p70S6K induces expression of matrix metalloproteinase 9 associated with hepatocyte growth factor-mediated invasion in human ovarian cancer cells, Endocrinology. 2006, 147: 2557-2566. |
| Zhou H. and Wong A.S.T., Up-Regulation of Matrix Metalloproteinase-9 Is Associated with Hepatocyte Growth Factor-Mediated Invasion in Human Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-30). 2006. |