DEPT OF OBSTETRICS & GYNAECOLOGY



Researcher : Chan CCW

List of Research Outputs

Chan C.C.W., Lau W.N.T., Chiu S.P., Chen L.C., Choi W.K. and Tang G.W.K., A pilot study on the effects of a Chinese herbal preparation on menopausal symptoms, Gynecological Endocrinology. 2006, 22(2): 70-73.
Chan C.C.W., Ng E.H.Y., Tang F.O.S., Yeung W.S.B., Lau E.Y.L. and Ho P.C., A prospective, randomized, double-blind study to compare two doses of recombinant human chorionic gonadotropin in inducing final oocyte maturity and the hormonal profile during the luteal phase, The Journal of Clinical Endocrinology and Metabolism. 2005, 90(7): 3933-3938.
Chan C.C.W., Tang F.O.S., Lau W.N.T. and Tang G.W.K., Bone turnover in young hypoestrogenic women on hormonal therapy, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 124: 204-206.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Circadian changes in uterine artery and ovarian stromal blood flow after pituitary down-regulation, Journal of The Society for Gynecology Investigation. 2005, 12(6): 452-455.
Chan C.C.W., Koo M.W.L., Ng E.H.Y., Tang F.O.S., Yeung W.S.B. and Ho P.C., Effects of Chinese green tea on weight, and hormonal and biochemical profiles in obese patients with polycystic ovary syndrome - A randomized placebo-controlled trial, Journal of The Society for Gynecology Investigation. 2006, 13(1): 64-68.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Endometrial, subendometrial, and ovarian stromal blood flow in patients treated with two different doses of recombinant human chorionic gonadotropin , Fertility and Sterility. 2006, 85(2): 520-522.
Chan C.C.W. and Ho P.C., Infertility, assisted reproduction and rights , Best Practice and Research Clinical Obstetrics and Gynaecology. 2006, 20(3): 369-380.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Content And 4977 bp Deletion In Unselected And Unfertilized Oocytes, Molecular Human Reproduction. 2006, 11: 843-846.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Deletion In Granulosa And Cumulus Oophorus Cells, Fertility and Sterility. 2006, 85: 780-782.
Chan C.C.W., Ng E.H.Y. and Ho P.C., Obesity and reproduction, Journal of Paediatrics, Obstetrics and Gynaecology. 2006, 32(3): 127-131.
Ho P.C., Chan C.C.W., Tang F.O.S. and Ng E.H.Y., Emergency contraception, Gynaecology Forum. 2005, 10(4): 16-18.
Ng E.H.Y., Chan C.C.W. and Ho P.C., Are there differences in ultrasound parameters between Chinese women with polycystic ovaries only and with polycystic ovary syndrome?, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 125: 92-98.
Ng E.H.Y., Chan C.C.W., Tang F.O.S. and Ho P.C., Comparison of endometrial and subendometrial blood flows among patients with and without hydrosalpinx shown on scanning during in vitro fertilization treatment, Fertility and Sterility. 2006, 85(2): 333-338.
Ng E.H.Y., Chan C.C.W., Yeung W.S.B. and Ho P.C., Comparison of ovarian stromal flow between fertile women with normal ovaries and infertile women with polycystic ovary syndrome, The 6th International Scientific Meeting, Cairo, Egypt, September 27-30, 2005.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Factors affecting endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound during IVF treatment, Human Reproduction. 2006, 21(4): 1062-1069.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal blood flow in the prediction of ovarian response during in vitro fertilization treatment, Human Reproduction. 2005, 20(11): 3147-3151.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal vascularity is not predictive of ovarian response and pregnancy, Reproductive BioMedicine Online. 2006, 12: 43-49.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Relationship between uterine blood flow and endometrial and subendometrial blood flows during stimulated and natural cycles, Fertility and Sterility. 2006, 85(3): 721-727.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during frozen-thawed embryo transfer cycles, Human Reproduction. 2006, 21(6): 1612-1617.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flows measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during IVF treatment, Human Reproduction. 2006, 21(1): 164-170.
Tang F.O.S., Chan C.C.W., Kan A.S.Y. and Ho P.C., A prospective randomized comparison of sublingual and oral misoprostol when combined with mifepristone for medical abortion at 12-20 weeks gestation, Presented at The 54th Meeting of the American Society of Reproductive Medicine, Montreal, Canada, October 15-20, 2005.
Tang F.O.S., Chan C.C.W., Kan A. and Ho P.C., A prospective randomized comparison of sublingual and oral misoprostol when combined with mifepristone for medical abortion at 12-20 weks gestationl, Human Reproduction. 2005, 20(11): 3062-3066.


Researcher : Chan CP

List of Research Outputs

Chan C.P., Leung W.C., Leung K.Y., Pun T.C. and Lee C.P., Confirmation of first trimester fetal abnormalities at termination of pregnancy, The 15th World Congress on Ultrasound in Obstetrics and Gynecology (ISUOG 2005), September 25-29, 2005, Vancouver, Canada. 2005.
Lao T.T.H., Chan C.P., Leung W.C. and Ho L.F., Maternal age and prevalence of gestational diabetes mellitus, Diabetes Care. 2006, 29: 948-949.


Researcher : Chan DW

Project Title:Characterization of the roles of Hepatitis B virus X protein in hepatocellular carcinoma
Investigator(s):Chan DW, Ng IOL
Department:Pathology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Completion Date:04/2006
Abstract:
To knock down the expression of HBx in an HBx-expressing HCC cell line (PLC/PRF/5), (RNAi); to investigate the expression levels of HBx-induced oncogenes in PLC/PRF/5 cells with knock-down of HBx; to evaluate the change of tumorigenicity of PLC/PRF/5 cells with knock-down of HBx using in vitro and in vivo assays.


List of Research Outputs



Researcher : Chan KK

List of Research Outputs

Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K., Methotrexate, bleomycin, and etoposide in the treatment of gestational trophoblastic neoplasia , Obstetrics and Gynecology. 2006, 107(5): 1012-1017.


Researcher : Chan KKL

Project Title:Relationship between placental ratio and placental function
Investigator(s):Chan KKL, Lao TTH
Department:Obstetrics & Gynaecology
Source(s) of Funding:Seed Funding for New Staff
Start Date:01/2003
Abstract:
To examine the relationship between PR and placental function in terms of fetal acid- base balance, haematology and biochemiatry; to determine if there is a cut-off in the PR for the detection of fetal growth restriction (FRG) as reflected by changes in placental function in newborns with birthweigth within the normal range.


Project Title:The role resistin in gestational diabetes and its relationship with pregnancy outcome
Investigator(s):Chan KKL, Lam KSL, Lao TTH, Tso A.W.K.
Department:Obstetrics & Gynaecology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Abstract:
To investigate the hypothesis that resistin expression is associated with insulin resistance in pregnancy and has implications on neonatal outcome.


Project Title:Human papillomavirus (HPV) genotyping spectrum in HPV infected patients undergoing cervical cytological sampling in Hong Kong
Investigator(s):Chan KKL, Ngan HYS, Cheung ANY, Chan YK
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Completion Date:04/2006
Abstract:
To investigate the human papillomavirus (HPV) spectrum in the HPV infected women undergoing routine liquid-based cervical cytological sampling and in women diagnosed to have cervical cancer in Hong Kong.


Project Title:Iron supplement in pregnancy and development of gestational diabetes - a randomized placebo-controlled trial
Investigator(s):Chan KKL
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
N/A


Project Title:Iron supplement in pregnancy and development of gestational diabetes - a randomized placebo-controlled trial
Investigator(s):Chan KKL, Lao TTH
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2005
Abstract:
To conduct a randomized placebo control trial to establish the relationship between iron store and development of GDM; to explore the mechanism by which a high iron load is related to GDM; to explore the relationship between iron store and liver production of insulin resistance; to explore the relationship between iron store and liver production of IGF which has similar action as insulin on glucose metabolism, whose level has been associated with development of disbetes.


Project Title:Oestrogen receptor subtypes status in ovarian cancer
Investigator(s):Chan KKL, Tam KF, Ngan HYS, Chan YK, Liu B
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:01/2006
Abstract:
Objective : To investigate the relationship between oestrogen receptors subtypes and clinical parameters in ovarian cancer Key issues and problem addressed: Ovarian cancer cells, like breast cancer cells, express oestrogen receptors (Rao 1991) and are oestrogen sensitive. Oestrogen is a steroid hormone, mainly synthesised in the ovary but also in peripheral tissues through aromatization of androgen (Korach KS 1996). It has diverse effects the reproductive system as well as other tissues such as the cardiovascular system and bone tissues. On the molecular level, oestrogen regulates the expression of many genes that are important for cell proliferation, inhibition of apoptosis, stimulation of invasion and metastasis and promotion of angiogenesis. Most of the effects of oestrogen are medicated by oestrogen receptors (ER). ER has at least 2 functions. Apart from being a transcription factor for oestrogen related genes, it also functions outside the nucleus and in the plasma membrane to active growth factor signalling ( Osborne CK 2005 ). Therefore, it can potentially affect tumour growth and clinical outcomes in ovarian cancer. The role of oestrogen receptors had been investigated in ovarian cancer. A number of earlier studies in the eighties and early nineties attempted to look at the relationship between presence of ER and survival but did not produce any firm conclusions ( Massood S 1989, Anderl P 1988, Rose P 1990, Geisler J, 1995 ). This may be due to the heterogeneous data set and the dextran coated charcoal adsorption assay used to detect ER which is now largely replaced by the more accurate immunohistochemical (IHC) method. Furthermore, In addition to the classical oestrogen receptor ( ER-a ), a second ER, ER- β was identified in 1996 (Mosselman S 1996 ). These both belong to a super family of nuclear hormone receptors but they are products of different genes. They have similar but not identical structures and they appear to have distinct functions from each other for example, ER- β seems to have opposing activity on tumour growth. These earlier studies did not differentiate between the presences of the receptor subtypes which may have an impact on the overall findings, particularly when the 2 subtypes appear to have opposing actions. Subsequent studies have attempted to look at the role of ER- β in ovarian cells. Hillier et al ( Hillier SG 1998 ) screened for ER-A and ER – β mRNA in primary benign ovarian epithelial cell cultures by RT-PCR in 4 women, using granulosa cells and granulosa –lutein cells as controls and found that these cells expresses mRNA for both subtypes. Brandenberger (Brandenberger 1998) measured the mRNA of ER- β expression in 10 normal ovaries and 10 serous cystadenocarcinoma as well as in normal and malignant ovarian cell lines. He found that ER- β was decreased in both ovarian cancer tissue and cell lines compared to normal. Li et al (Li 2003 ) compared ER a and β mRNA in primary cell cultures of normal ovarian epithelium ( n=23 ) verses in ovarian cancer ( n= 23 ) and found that Ratio of ER a / ER βwas 10 times higher in Ca ovary. Bardin et al (Bardin 2004) showed in 58 women that ER- β expression was were reduced during tumour progression and they restored ER- β expression in ovarian cancer cell line and found that ER-B expression strongly inhibit cell proliferation. Lindgren et al (2004) also looked at ER subtypes expression in ovarian tumours by using immunostaining rather then by measuring mRNA expression by RT-PCR and confirmed that ER-B immunuoreactivity was lower in epithelial cells in ovarian cancer than in normal ovaries. From these studies, it appears that the level of ER- β expression would be an important factor in ovarian carcinogenesis and may have implications on response to treatment and clinical outcomes. Therefore in this study, we would like to correlate the ER a and β status with clinical parameters and to attempt to answer the following questions: How does receptor subtype status correlate • with different histological types of ovarian cancer • with response to chemotherapy • with progression free interval References Anderl P. Fuith LC. Daxenbichler G. Marth C. Dapunt O. Correlation between steroid hormone receptors, histological and clinical parameters in ovarian carcinoma. Gynecologic & Obstetric Investigation. 25(2):135-40, 1988. Bardin A. Hoffmann P. Boulle N. Katsaros D. Vignon F. Pujol P. Lazennec G. Involvement of estrogen receptor beta in ovarian carcinogenesis. Cancer Research. 64(16):5861-9, 2004 Aug 15. Brandenberger AW. Tee MK. Jaffe RB. Estrogen receptor alpha (ER-alpha) and beta (ER-beta) mRNAs in normal ovary, ovarian serous cystadenocarcinoma and ovarian cancer cell lines: down-regulation of ER-beta in neoplastic tissues. Journal of Clinical Endocrinology & Metabolism. 83(3):1025-8, 1998 Mar Hillier SG. Anderson RA. Williams AR. Tetsuka M. Expression of oestrogen receptor alpha and beta in cultured human ovarian surface epithelial cells. [Journal Article] Molecular Human Reproduction. 4(8):811-5, 1998 Aug. Korach KS, Migiaccio S, Davis VL. Estrogen. In: Munson PL, eds Principles of Pharmacology. New York: Chapman & Hall, 1996; 809-25 Li AJ. Baldwin RL. Karlan BY. Estrogen and progesterone receptor subtype expression in normal and malignant ovarian epithelial cell cultures. American Journal of Obstetrics & Gynecology. 189(1):22-7, 2003 Jul Masood S, Heitmann J, Nuss R, Bernrubi G. Clinical correlation of hormone receptor status in epithelial ovarian cancer. Gynecol Oncol 1989; 34: 57-60 Mosselman S, Polman J, Dijkema R. ER beta: identification and characterization of a novel human estrogen receptor. FEBS Lett, 392: 49-53, 1996. Osborne CK. Schiff R. Estrogen-receptor biology: continuing progress and therapeutic implications. Journal of Clinical Oncology. 23(8):1616-22, 2005 Mar 10. Perez-Gracia JL. Carrasco EM. Tamoxifen therapy for ovarian cancer in the adjuvant and advanced settings: systematic review of the literature and implications for future research Gynecologic Oncology. 84(2):201-9, 2002 Feb. Rao BR, Slotman BJ. Endocrine factors in common epithelial ovarian cancer. Endocr Rev, 12: 14-26, 1991


List of Research Outputs

Chan K.K.L., Lam K.W., Tam K.F. and Ngan H.Y.S., Effectiveness of a single dose methotrexate regime in treatment of low risk gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Chan K.K.L. and Lao T.T.H., Fetal haematocrit is a determinant of placental size in term pregnancies, Acta Haematologica. 2006, 116: 41-45.
Chan K.K.L. and Ngan H.Y.S., Should women with treated genital tract cancers start hormone replacement?, Journal of Paediatrics, Obstetrics and Gynaecology. 2005, Nov/Dec: 245248.
Ip P.P.C., Chan K.K.L., Cheung A.N.Y. and Ngan H.Y.S., Choriocarcinomas following full-term non-molar gestations, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Leung K.Y., Chan K.K.L., Ngai C.S.W., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.
Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K.L., Methotrexate, bleomycin and etoposide (MBE) in the treatment of gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Ngan H.Y.S., Lam K.W., Tam K.F. and Chan K.K.L., Treatment of recurrent gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Tse K.Y., Chan K.K.L., Tam K.F. and Ngan H.Y.S., Local experience of the management of profuse bleeding in gestational trophoblastic diseases, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.


Researcher : Chan YK

Project Title:Study of metastasis suppressing genes in metastatic ovarian cancer
Investigator(s):Chan YK, Ngan HYS, Cheung ANY, Khoo US
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Completion Date:04/2006
Abstract:
To investigate the role of the metastasis suppressing genes in the metastasis ovarian cancer. The epigenetic factor of promoter hypermethylation in relation to the expression of the metastasis suppressing gene will aso be investigated.


List of Research Outputs



Researcher : Chan YM

List of Research Outputs

Chan Y.M., Lee P.W.H., Fong D.Y.T., Fung A.S.M., Wu L.Y.F., Choi A.Y., Ng T.Y., Ngan H.Y.S. and Wong L.C., Effect of individual psychological intervention in Chinese women with gynecologic malignancy: A randomized controlled trial, Journal of Clinical Oncology. 2005, 23(22): 4913-4924.
Tam K.F., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Ethnicity is a factor to be considered before dose planning in ovarian cancer patients to be treated with topotecan, International Journal of Gynecological Cancer. 2006, 16: 135-139.
Tam K.F., Chau M.T., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Hepatic arteriogram for gestational trophoblastic tumor: Is it useful? , Cancer Investigation. 2005, 23: 677-682.


Researcher : Chen M

List of Research Outputs

Chen M., Leung K.Y., Lee C.P., Tang M.H.Y. and Ho P.C., Placental volume measured by three-dimensional ultrasound in the prediction of fetal ao-thalassemia: a preliminary report, Ultrasound Obstetrics and Gynecology. 2006, 28: 166-172.
Chen M., Chan G.S., Lee C.P. and Tang M.H.Y., Sonographic features of lethal multiple pterygium syndrome at 14 weeks, Prenatal Diagnosis. 2005, 25: 475-478.


Researcher : Cheng DKL

List of Research Outputs

Tam K.F., Cheng D.K.L., Ng T.Y. and Ngan H.Y.S., The behaviors of seeking a second opinion from other health-care professionals and the utilization of complementary and alternative medicine in gynecologic cancer patients, Support Care Cancer. 2005, 13: 679-684.


Researcher : Chiu CN

Project Title:Prevalence of Y-chromosome AZFd microdeletion in Hong Kong men with male infertility
Investigator(s):Chiu CN, Ng EHY, Lee CKF, Yeung WSB
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To determine the prevalence of AZFd deletion in a Chinese population in Hong Kong, particularly in a group of men with abnormal sperm morphology. Abnormal sperm morphology is one of the proposed characteristics of men having AZFd deletion.


List of Research Outputs

Chiu C.N., Chung M.K., Lee C.K.F., Ho P.C. and Yeung W.S.B., Adrenomedullin effects on sperm functions. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Chiu C.N., Chung M.K., Tsang H.Y., Koistinen R., Koistinen H., Seppala M., Lee C.K.F. and Yeung W.S.B., Glycodelin-S in human seminal plasma reduces cholesterol efflux and inhibits capacitation of spermatozoa, The Journal of Biological Chemistry. 2005, 280(27): 25580-25589.
Chung M.K., Chiu C.N., Lee C.K.F., Ho P.C. and Yeung W.S.B., A role for human sperm surface fucosyltransferase in glycodelin binding., International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006, P.10.
Chung M.K., Chiu C.N., Lee C.K.F., Yeung W.S.B. and Ho P.C., Biological and biochemical characterization of the glycodelin isoform from human cumulus cell matrix. P5.38, Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005.
Lee C.K.F., Cheung W.K.L., Chung M.K., Chiu C.N., Yeung W.S.B. and Ng E.H.Y., Proteomic profiling of the uterine flushing from natural and stimulated cycles during peri-implantation period., Society for the Study of Reproduction -38th Annual Meeting. 24-27 Jul, Quebec, Canada. . 2005.
Seppala M., Koistinen R., Koistinen H., Chiu C.N. and Yeung W.S.B., Glycodelin:A Lipocalin with Diverse Glycoform-Dependent Actions, In: Akerström B, N. Borregaard, D.R. Flower Edward, J.P. Salier, Lipocalins. USA, Landes Bioscience, 2006, 121-130.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Glycodelin: A molecule with multi-functions on spermatozoa. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Roles of glycodelin in modulating sperm function, Molecular and Cellular Endocrinology. 2006, 250: 149-56.


Researcher : Choi WK

List of Research Outputs

Chan C.C.W., Lau W.N.T., Chiu S.P., Chen L.C., Choi W.K. and Tang G.W.K., A pilot study on the effects of a Chinese herbal preparation on menopausal symptoms, Gynecological Endocrinology. 2006, 22(2): 70-73.


Researcher : Chow JFC

List of Research Outputs

Lee C.K.F., Leung K.Y., Chung M.K., Lee C.Y.L., Chow J.F.C. and Yeung W.S.B., Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct, Journal of Cellular Biochemistry. 2005, 95: 740-749.


Researcher : Chung MK

List of Research Outputs

Chiu C.N., Chung M.K., Lee C.K.F., Ho P.C. and Yeung W.S.B., Adrenomedullin effects on sperm functions. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Chiu C.N., Chung M.K., Tsang H.Y., Koistinen R., Koistinen H., Seppala M., Lee C.K.F. and Yeung W.S.B., Glycodelin-S in human seminal plasma reduces cholesterol efflux and inhibits capacitation of spermatozoa, The Journal of Biological Chemistry. 2005, 280(27): 25580-25589.
Chung M.K., Chiu C.N., Lee C.K.F., Ho P.C. and Yeung W.S.B., A role for human sperm surface fucosyltransferase in glycodelin binding., International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006, P.10.
Chung M.K., Chiu C.N., Lee C.K.F., Yeung W.S.B. and Ho P.C., Biological and biochemical characterization of the glycodelin isoform from human cumulus cell matrix. P5.38, Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005.
Lee C.K.F., Leung K.Y., Chung M.K., Lee C.Y.L., Chow J.F.C. and Yeung W.S.B., Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct, Journal of Cellular Biochemistry. 2005, 95: 740-749.
Lee C.K.F., Cheung W.K.L., Chung M.K., Chiu C.N., Yeung W.S.B. and Ng E.H.Y., Proteomic profiling of the uterine flushing from natural and stimulated cycles during peri-implantation period., Society for the Study of Reproduction -38th Annual Meeting. 24-27 Jul, Quebec, Canada. . 2005.


Researcher : Chung MK

List of Research Outputs

Chiu C.N., Chung M.K., Lee C.K.F., Ho P.C. and Yeung W.S.B., Adrenomedullin effects on sperm functions. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Chiu C.N., Chung M.K., Tsang H.Y., Koistinen R., Koistinen H., Seppala M., Lee C.K.F. and Yeung W.S.B., Glycodelin-S in human seminal plasma reduces cholesterol efflux and inhibits capacitation of spermatozoa, The Journal of Biological Chemistry. 2005, 280(27): 25580-25589.
Chung M.K., Chiu C.N., Lee C.K.F., Ho P.C. and Yeung W.S.B., A role for human sperm surface fucosyltransferase in glycodelin binding., International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006, P.10.
Chung M.K., Chiu C.N., Lee C.K.F., Yeung W.S.B. and Ho P.C., Biological and biochemical characterization of the glycodelin isoform from human cumulus cell matrix. P5.38, Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005.
Lee C.K.F., Leung K.Y., Chung M.K., Lee C.Y.L., Chow J.F.C. and Yeung W.S.B., Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct, Journal of Cellular Biochemistry. 2005, 95: 740-749.
Lee C.K.F., Cheung W.K.L., Chung M.K., Chiu C.N., Yeung W.S.B. and Ng E.H.Y., Proteomic profiling of the uterine flushing from natural and stimulated cycles during peri-implantation period., Society for the Study of Reproduction -38th Annual Meeting. 24-27 Jul, Quebec, Canada. . 2005.


Researcher : Ho PC

Project Title:A randomized trial to compare the use of sublingual misoprostol with or without an extended one-week course in the management of first trimester silent miscarriage
Investigator(s):Ho PC, Ong CYT, Tang FOS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Completion Date:10/2005
Abstract:
To study the effect of a 1-week course of sublingual misoprostol on the incidence of complete miscarriage after medical management of silent miscarriage.


Project Title:Role of glycodelin in fertilization
Investigator(s):Ho PC, Lee CKF, Yeung WSB, Seppala M, Koistinen RA, Dell A.
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:12/2003
Completion Date:12/2006
Abstract:
To establish a 3D capillary cumulus cell-matrix model to study the effect of the cumulus cell mass on the spermatozoa passing through it; to study the effect of GdA and GdF on the quality of spermatozoa passing through the cumulus mass; to study the metabolism of GdA and Gdf in cumulus cells; and to study the production of GdA and GdF by the granulosa cells, endometrial cells and oviducal cells.


Project Title:Role of glycodelin in fertilization
Investigator(s):Ho PC, Lee CKF, Yeung WSB, Seppala M, Koistinen RA, Dell A.
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:12/2003
Completion Date:12/2005
Abstract:
N/A


Project Title:Endocrine gland derived vascular endothelial growth factor (EG-VEGF) in human endometrium
Investigator(s):Ho PC, Ngan ESW, Ng EHY, Yeung WSB
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:10/2004
Abstract:
To examine the temporal and spatial expression pattern of EG-VEGF and its receptors in human endometrium; to elucidate the mitogenic and angiogenic properties of EG-VEGF in human endometrial microvascular endothelial cell (hEMVEC); to study the expression of EG-VEGF in patients with endometriosis.


Project Title:A pilot study on the use of danazol in combination with vaginal misoprostol in termination of pregnancies less than 9 weeks of gestation
Investigator(s):Ho PC, Chan CW, Tang OS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Completion Date:10/2005
Abstract:
To further explore the effect of danazol in combination with misoprostol in terminating first trimester pregnancies.


Project Title:Endocrine gland derived vascular endothelial growth factor (EG-VEGF) in human endometrium
Investigator(s):Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
N/A


Project Title:Will letrozole improve the ovarian response in women with poor ovarian reserve who are undergoing IVF treatment?
Investigator(s):Ho PC, Chan CCW, Ng EHY, Tang OS, Yeung WSB, Lau E
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:07/2005
Abstract:
Letrozole is a non-steroidal reversible, competitive aromatase inhibitor that is highly potent and selective. It has a short half-life of around 45 hours (Sioufi et al., 1997). The rapid elimination from the body and the absence of estrogen receptor down-regulation effect as observed in clomiphene citrate treatment allow adequate time for the endometrium to regenerate from the anti-estrogen effect of the drug and therefore enhances implantation. The mechanism of action of letrozole in ovulation induction is proposed to be due to the selective inhibition of the aromatase enzyme that catalyzes the rate-limiting step in the conversion of androstenedione and testosterone to estrone and estradiol respectively. Treatment with an aromatase inhibitor in the early part of the menstrual cycle will decrease estrogen synthesis and hence the negative feedback centrally, resulting in increased gonadotrophin secretion. Moreover, by blocking the conversion of androgens to estrogens in the ovary, the accumulating intra-ovarian androgens may increase follicular sensitivity through amplification of FSH receptor gene expression (Weil et al., 1998; 1999; Vendola et al., 1998; 1999).


Project Title:Role of olfactomedin in implantation
Investigator(s):Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2006
Abstract:
N/A


Project Title:Role of olfactomedin in implantation
Investigator(s):Ho PC, Lee CKF, Ng EHY, Yeung WSB
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2006
Abstract:
To study the expression of olfactomedin in different phases of the reproductive cycle; to determine the site of olfactomedin biosynthesis; to study the role of steroid in the regulation of olfactomedin biosynthesis; to study the role of the molecule in embryo adhesion and trophoblast invasion.


List of Research Outputs

Chan C.C.W., Ng E.H.Y., Tang F.O.S., Yeung W.S.B., Lau E.Y.L. and Ho P.C., A prospective, randomized, double-blind study to compare two doses of recombinant human chorionic gonadotropin in inducing final oocyte maturity and the hormonal profile during the luteal phase, The Journal of Clinical Endocrinology and Metabolism. 2005, 90(7): 3933-3938.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Circadian changes in uterine artery and ovarian stromal blood flow after pituitary down-regulation, Journal of The Society for Gynecology Investigation. 2005, 12(6): 452-455.
Chan C.C.W., Koo M.W.L., Ng E.H.Y., Tang F.O.S., Yeung W.S.B. and Ho P.C., Effects of Chinese green tea on weight, and hormonal and biochemical profiles in obese patients with polycystic ovary syndrome - A randomized placebo-controlled trial, Journal of The Society for Gynecology Investigation. 2006, 13(1): 64-68.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Endometrial, subendometrial, and ovarian stromal blood flow in patients treated with two different doses of recombinant human chorionic gonadotropin , Fertility and Sterility. 2006, 85(2): 520-522.
Chan C.C.W. and Ho P.C., Infertility, assisted reproduction and rights , Best Practice and Research Clinical Obstetrics and Gynaecology. 2006, 20(3): 369-380.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Content And 4977 bp Deletion In Unselected And Unfertilized Oocytes, Molecular Human Reproduction. 2006, 11: 843-846.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Deletion In Granulosa And Cumulus Oophorus Cells, Fertility and Sterility. 2006, 85: 780-782.
Chan C.C.W., Ng E.H.Y. and Ho P.C., Obesity and reproduction, Journal of Paediatrics, Obstetrics and Gynaecology. 2006, 32(3): 127-131.
Chan C.H.Y., Chan C.L.W., Ng S.M., Ng E.H.Y. and Ho P.C., Body-mind-spirit intervention for IVF women, Journal of Assisted Reproduction and Genetics. 2005, 22(11&12): 419-427.
Chan C.H.Y., Ng E.H.Y., Chan C.L.W., Ho P.C. and Chan H.Y., Effectiveness of psychosocial group intervention for reducing anxiety in women undergoing in vitro fertilization: A randomized controlled study, Fertility and Sterility. 2006, 85(2): 339-346.
Chen M., Leung K.Y., Lee C.P., Tang M.H.Y. and Ho P.C., Placental volume measured by three-dimensional ultrasound in the prediction of fetal ao-thalassemia: a preliminary report, Ultrasound Obstetrics and Gynecology. 2006, 28: 166-172.
Chiu C.N., Chung M.K., Lee C.K.F., Ho P.C. and Yeung W.S.B., Adrenomedullin effects on sperm functions. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Chung M.K., Chiu C.N., Lee C.K.F., Ho P.C. and Yeung W.S.B., A role for human sperm surface fucosyltransferase in glycodelin binding., International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006, P.10.
Chung M.K., Chiu C.N., Lee C.K.F., Yeung W.S.B. and Ho P.C., Biological and biochemical characterization of the glycodelin isoform from human cumulus cell matrix. P5.38, Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005.
Ho P.C., Commentary on article "Comparison of oral and vaginal misoprostol for cervical ripening before manual vacuum aspiration of first trimester pregnancy under local anesthesia: a randomized placebo-controlled study, Contraception 2005;71:337-342", Evidence-based Obstetrics and Gynecology. 2006, 8: 32-33.
Ho P.C., Chan C.C.W., Tang F.O.S. and Ng E.H.Y., Emergency contraception, Gynaecology Forum. 2005, 10(4): 16-18.
Ho P.C., Emergency contraception, Gynecological Endocrinology. 2006, 22(Supplement 1): 68.
Ho P.C., Emergency contraception, The 12th World Congress of Gynecological Endocrinology, Florence, Italy, March 2-5, 2006. 2006.
Ho P.C., Family planning, Seminar at The 1st Beijing International Conference on Obstetrics and Gynecology, October 7-10, 2005, Beijing, China. 2005.
Ho P.C., Making abortions safer, Plenary lecture presented at The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005. 2005.
Ho P.C., Medical abortion, Plenary lecture presented at The 1st Beijing International Conference on Obstetrics and Gynecology, October 7-10, 2005, Beijing, China. 2005.
Ho P.C., Obesity and assisted reproduction, 5th International Conference on Reproductive Endocrinology, 9-12 September 2005. Beijing, China, 2005.
Ho P.C., Ovarian stimulation and endometrial receptivity, Asia Pacific Initiative on Reproductive Endocrinology: Inaugural Congress, Optimizing infertility management in Asia, Changsha, Hunan, China, 31 March-2 April, 2006. 2006.
Ho P.C., State of art in fertility regulation technology, The 3rd Asia Pacific Conference on Reproductive and Sexual Health, Selangor Darul Ehsan, Malaysia, 17-21 November 2005. 2005.
Ho P.C., Strategies for treating hyper/hyporesponders; medical treatment, Asia Pacific Initiative on Reproductive Endocrinology: Inaugural Congress, Optimizing infertility management in Asia, Changsha, Hunan, China, 31 March-2 April, 2006. 2006.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, Prenatal Diagnosis. 2006, 26: 510-513.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, The 13th International Conference on Prenatal Diagnosis and Therapy, Kyoto, Japan, 28 - 31 May. 2006.
Hui P.W., Lee C.P., Tang M.H.Y. and Ho P.C., Nuchal translucency in pregnancies conceived after assisted reproduction technology, Current Opinion in Obstetrics and Gynecology. 2006, 18: 319-324.
Kan A., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Perifollicular vascularity in poor ovarian responders during IVF, Human Reproduction. 2006, 21(6): 1539-1544.
Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyper-stimulated cycle during in vitro fertilization treatment. , In: Liu Y, Lee KF, Ng EHY, Ho PC , Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005, P5.39.
Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyperstimulated cycle during in vitro fertilization treatment, 38th Annual Meeting, Society for The Study of Reproduction, Quebec, Canada, July 24-27, 2005.
Makkar G., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Reduced expression of interleukin-11 and interleukin-6 in the peri-implantation endometrium of excessive ovarian responders during in-vitro fertilization treatment, Journal of Clinical Endocrinology and Metabolism. 2006, 91(8): 3181-3188.
Ng E.H.Y., Chan C.C.W. and Ho P.C., Are there differences in ultrasound parameters between Chinese women with polycystic ovaries only and with polycystic ovary syndrome?, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 125: 92-98.
Ng E.H.Y., Chan C.C.W., Tang F.O.S. and Ho P.C., Comparison of endometrial and subendometrial blood flows among patients with and without hydrosalpinx shown on scanning during in vitro fertilization treatment, Fertility and Sterility. 2006, 85(2): 333-338.
Ng E.H.Y., Chan C.C.W., Yeung W.S.B. and Ho P.C., Comparison of ovarian stromal flow between fertile women with normal ovaries and infertile women with polycystic ovary syndrome, The 6th International Scientific Meeting, Cairo, Egypt, September 27-30, 2005.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Factors affecting endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound during IVF treatment, Human Reproduction. 2006, 21(4): 1062-1069.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal blood flow in the prediction of ovarian response during in vitro fertilization treatment, Human Reproduction. 2005, 20(11): 3147-3151.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal vascularity is not predictive of ovarian response and pregnancy, Reproductive BioMedicine Online. 2006, 12: 43-49.
Ng E.H.Y. and Ho P.C., Progestogen as Emergency Contraception, Royal College of Obstetrics and Gynaecology, Dialog. 2005, 4: 2.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Relationship between uterine blood flow and endometrial and subendometrial blood flows during stimulated and natural cycles, Fertility and Sterility. 2006, 85(3): 721-727.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during frozen-thawed embryo transfer cycles, Human Reproduction. 2006, 21(6): 1612-1617.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flows measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during IVF treatment, Human Reproduction. 2006, 21(1): 164-170.
Ng E.H.Y., Yeung W.S.B. and Ho P.C., The significance of antral follicle count in controlled ovarian stimulation and intrauterine insemination, Journal of Assisted Reproduction and Genetics. 2005, 22: 323-328.
Ngan E.S.W., Lee K.Y., Yeung W.S.B., Ngan H.Y.S., Ng E.H.Y. and Ho P.C., Endocrine Gland-Derived Vascular Endothelial Growth Factor is expressed in human peri-implantation endometrium, but not in endometrial carcinoma, Endocrinology. 2006, 147: 88-95.
Tang F.O.S., Chan C.C.W., Kan A.S.Y. and Ho P.C., A prospective randomized comparison of sublingual and oral misoprostol when combined with mifepristone for medical abortion at 12-20 weeks gestation, Presented at The 54th Meeting of the American Society of Reproductive Medicine, Montreal, Canada, October 15-20, 2005.
Tang F.O.S., Chan C.C.W., Kan A. and Ho P.C., A prospective randomized comparison of sublingual and oral misoprostol when combined with mifepristone for medical abortion at 12-20 weks gestationl, Human Reproduction. 2005, 20(11): 3062-3066.
Tang F.O.S., Ong C.Y.T., Tse K.Y., Ng E.H.Y., Lee W.H. and Ho P.C., A randomized trial to compare the use of sublingual misoprostol with or without an additional 1 week course for the management of first trimester silent miscarriage, Human Reproduction. 2006, 21(1): 189-192.
Tang F.O.S. and Ho P.C., Medical management of first-trimester miscarriages, Journal of Paediatrics, Obstetrics and Gynaecology. 2006, 32(3) May/Jun: 102-105.
Tang F.O.S. and Ho P.C., Termination of pregnancy, In: Edited by J.M.G. van Vugt, L.P. Shulman, Prenatal Medicine. New York, London, Taylor & Francis, 2005, Chapter 28: 509-520.
Tiwari A.F.Y., Leung W.C., Leung T.W., Humphreys J., Parker B. and Ho P.C., A randomised controlled trial of empowerment training for Chinese abused pregnant women in Hong Kong, British Journal of Obstetrics & Gynaecology. 2005, 112: 1249-56.
Tiwari A.F.Y., Leung W.C., Leung T.W., Humphreys J., Parker B. and Ho P.C., A randomised trial of empowerment training for Chinese abused pregnant women in Hong Kong, Frontiers in Biomedical Research: Achieving Excellence through Multi-disciplinary Research, Faculty of Medicine, The University of Hong Kong. Hong Kong, 2005, p.81.
Tiwari A.F.Y., Chan E.K.L., Leung W.C., Fong D.Y.T. and Ho P.C., The effectiveness of the Chinese version of the Abuse Assessment Screen to identify abused women, the Hospital Authority Convention 2006: From Policy to Practice, organized by the Hospital Authority, Hong Kong. 2006.
Tiwari A.F.Y., Chan E.K.L., Leung W.C., Fong D.Y.T. and Ho P.C., The prevalence of intimate partner violence among pregnant women, the Hospital Authority Convention 2006: From Policy to Practice, organized by the Hospital Authority, Hong Kong. 2006.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Glycodelin: A molecule with multi-functions on spermatozoa. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Roles of glycodelin in modulating sperm function, Molecular and Cellular Endocrinology. 2006, 250: 149-56.


Researcher : Huang FY

List of Research Outputs

Huang F.Y., Chiu P.M., Tam K.F., Kwok K.Y., Lau E.T., Tang M.H., Ng T.Y., Liu V.W.S., Cheung A.N.Y. and Ngan H.Y.S., Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer, Gynecologic Oncology. 2006, 103(1): 219-25.


Researcher : Hui PW

List of Research Outputs

Hui P.W., A few words of encouragement to MRCOG candidates, Hong Kong Journal of Gynaecology, Obstetrics and Midwifery. Hong Kong Academy of Medicine Press, 2005, 5(1): 6.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, Prenatal Diagnosis. 2006, 26: 510-513.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, The 13th International Conference on Prenatal Diagnosis and Therapy, Kyoto, Japan, 28 - 31 May. 2006.
Hui P.W., Lee C.P., Tang M.H.Y. and Ho P.C., Nuchal translucency in pregnancies conceived after assisted reproduction technology, Current Opinion in Obstetrics and Gynecology. 2006, 18: 319-324.


Researcher : Kwok KY

List of Research Outputs

Huang F.Y., Chiu P.M., Tam K.F., Kwok K.Y., Lau E.T., Tang M.H., Ng T.Y., Liu V.W.S., Cheung A.N.Y. and Ngan H.Y.S., Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer, Gynecologic Oncology. 2006, 103(1): 219-25.
Lau E.T.K., Kwok K.Y., Leung K.Y. and Tang M.H.Y., Automating non-invasive prenatal diagnosis of HB Bart's disease on immunofluorescence staining of fetal erythrocytes in maternal blood, The 13th Conference on Prenatal Diagnosis and Therapy, May 28-31, 2006, Kyoto, Japan. 2006.


Researcher : Lam HSW

List of Research Outputs

Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., Amniotic fluid volume at 41 weeks and infant outcome, Journal of Reproductive Medicine. 2006, 51: 484-488.
Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., The use of fetal Doppler cerebroplacental blood flow and amniotic fluid volume measurement in the surveillance of postdated pregnancies, In: Lam H, Leung WC, Lee CP, Lao TT, Acta Obstetricia et Gynecologica Scandinavica. 2005, 84: 844-848.
Leung W.C., Lam H.S.W., Lao T.T.H. and Tang M.H.Y., Biochemical hypothyroidism-A new finding in morror syndrome?, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 125(2): 269-271.


Researcher : Lam KW

List of Research Outputs

Chan K.K.L., Lam K.W., Tam K.F. and Ngan H.Y.S., Effectiveness of a single dose methotrexate regime in treatment of low risk gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Lam K.W., Wong H.S. and Pun T.C., The practice of episiotomy in public hospitals in Hong Kong, Hong Kong Medical Journal. 2006, 12(2): 94-98.
Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K.L., Methotrexate, bleomycin and etoposide (MBE) in the treatment of gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K., Methotrexate, bleomycin, and etoposide in the treatment of gestational trophoblastic neoplasia , Obstetrics and Gynecology. 2006, 107(5): 1012-1017.
Ngan H.Y.S., Lam K.W., Tam K.F. and Chan K.K.L., Treatment of recurrent gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.


Researcher : Lao TTH

Project Title:Dietary caloric intake and the development of gestational diabetes mellitus in at-risk Chinese women
Investigator(s):Lao TTH, Leung WC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2002
Abstract:
To determine the relationship between daily total caloric intake in the third trimester and the development of gestational diabetes mellitus in at-risk pregnant Chinese women; to compare the roles of toal caloric intake versus the carbohydrate: protein: fat ratio in the daily diet and development of gestational diabetes mellitus in the at-risk Chinese women.


Project Title:The role of adiponectin in the development of gestational diabetes mellitus and its effect on pregnancy outcome
Investigator(s):Lao TTH, Lam KSL, Chan KKL, Tso A.W.K.
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Abstract:
To determine if plasma adiponectin concentration is reduced, at the time of the oral glucose tolerance test (OGTT), in women who develop GDM in the latter half of pregnancy versus women with normal OGTT; to determine, following the diagnosis of GDM, whether the subsequent changes in adiponectin concentration is associated with obstetric complications and perinatal morbidity; to correlate circulatory concentration of adiponectin with polymorphisms and mutations in the apM1 gene in high risk women with and without GDM, and with the expression of adiponectin in subcutaneous and omental fat cells obtained from biopsy at the time of caesarean delivery for obstetric indications; to correlate antenatal adiponectin concentration and result of the genetic study with the postnatal glucose tolerance status in the women who have developed GDM.


Project Title:A study on the interrelationship among insulin-like growth factors, apoptosis and proliferation of trophoblast, and placental size throughout pregnancy, and their effects on infant outcome, in normal and diabetic pregnancies
Investigator(s):Lao TTH, Cheung PT, Cheung ANY, Leung WC, Lam HSW
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:12/2003
Abstract:
To compare in the normal and diabetic pregnancies the relationship between ultrasound-estimated placenta size and its vasculature, and fetal size, with maternal circulatory levels of IGFs and IGFBPs in the three trimesters; to compare in the normal and diabetic pregnancies the relationship among placental expression of IGFs with placental apoptotic and proliferative indices and placental size at the time of delivery; to determine whether the interactions between IGFs and IGFBPs with placental growth and size in the different trimesters can be predictive of obstetric and perinatal complications; to determine whether the interactions between IGFs and IGFBPs with placental growth and size in the different trimesters can be predictive of fetal growth and size at birth; to examine the relationship between placental and newborn size with the placental expression of IGFs as well as the apoptotic and proliferative indices in normal and diabetic pregnancies.


Project Title:A study on the interrelationship among insulin-like growth factors, apoptosis and proliferation of trophoblast, and placental size throughout pregnancy, and their effects on infant outcome, in normal and diabetic pregnancies
Investigator(s):Lao TTH, Cheung PT, Cheung ANY, Leung WC, Lam HSW
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:12/2003
Abstract:
N/A


Project Title:Elevated TNF-α in gestational diabetes mellitus-cause or consequence?
Investigator(s):Lao TTH, Leung WC, Ngai CSW
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To determine and compare the serial changes in maternal blood level of TNF-α and its receptors, C-reactive protein (CRP) , and ferritin in relation to the development of gestational diabetes mellitus (GDM) in Chinese pregnant women with singleton and twin pregnancies; to determine the relationship between changes in maternal blood level of TNF-α and its receptors, CRP and ferritin with the occurrence of complicantions in Chinese pregnant women with and with and without GDM, and whether there is any difference between singleton and twin pregnancies. To correlate the levels of TNF-α and its receptors between maternal blood with cord blood, and between these two compartments with placental size and the placental expression of TNF-α and its receptors. To correlate maternal and cord blood, and placental TNF-α and its receptors with the infant birthweight and development of perinatal complications, and the determine if there is any difference between singleton and twin pregnancies.


Project Title:The relationship between the antioxidant defense system with the development of gestational diabetes mellitus and its complications in the Chinese population.
Investigator(s):Lao TTH, choy my, Wong NS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:12/2005
Abstract:
Gestational diabetes mellitus (GDM) has become the leading medical complication in pregnancy in the local Chinese population, and despite satisfactory glycaemic control, maternal and perinatal complications cannot be eliminated. One of the metabolic disturbances now documented with diabetes mellitus in non-pregnant subjects is an increased oxidative stress. The standard approach in the management of GDM is to normalize blood glucose level with dietary and insulin treatment, but this may not be sufficient to counter the increased oxidative stress, and the persistent risk of maternal and perinatal complications could be related to inadequate defense against the increased oxidative stress in GDM. There has been minimal information about the natural antioxidant defense systems, such as the enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and especially thioredoxin reductase (TRx), in pregnancy with and without GDM, and there is no information on these systems in the infant at the time of birth. These enzyme systems are present in the placenta and can also be measured in peripheral blood. The present study is designed to investigate (1) the maternal antioxidant defense systems at the time of diagnosis of GDM, comparing between women with normal and abnormal glucose tolerance; (2) and its subsequent changes in relation to treatment and glycaemic control, (3) the changes in relation to pregnancy complications in women with and without GDM; and (4) the changes in the fetal compartment as reflected in cord blood and placental tissue.


List of Research Outputs

Chan K.K.L. and Lao T.T.H., Fetal haematocrit is a determinant of placental size in term pregnancies, Acta Haematologica. 2006, 116: 41-45.
Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., Amniotic fluid volume at 41 weeks and infant outcome, Journal of Reproductive Medicine. 2006, 51: 484-488.
Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., The use of fetal Doppler cerebroplacental blood flow and amniotic fluid volume measurement in the surveillance of postdated pregnancies, In: Lam H, Leung WC, Lee CP, Lao TT, Acta Obstetricia et Gynecologica Scandinavica. 2005, 84: 844-848.
Lao T.T.H., Gestational diabetes: Current understanding and management, Medical Progress. 2005, September/2005: 443-448.
Lao T.T.H., Invited External Examiner for Macau Examination Board of Obstetrics and Gynaecology, Macau. 2005.
Lao T.T.H., Management of hyperthyroidism and goitre in pregnancy, and postpartum thyroiditis , Journal of Paediatrics, Obstetrics and Gynaecology. 2005, 31: 155-164.
Lao T.T.H., Chan C.P., Leung W.C. and Ho L.F., Maternal age and prevalence of gestational diabetes mellitus, Diabetes Care. 2006, 29: 948-949.
Lao T.T.H., Maternal age and reproductive outcome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.
Leung W.C., Lam H.S.W., Lao T.T.H. and Tang M.H.Y., Biochemical hypothyroidism-A new finding in morror syndrome?, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 125(2): 269-271.
Leung W.C. and Lao T.T.H., Rapid aneuploidy testing, traditional karyotyping, or both?, In: Leung WC, Lao TT, The Lancet. 2005, 366(9480): 97-98.
Liu R., Kwok Y.L., Li Y., Lao T.T.H. and Zhang X., Effects of material properties and fabric structure characteristics of graduated compression stockings (GCS) on the skin pressure distributions, Fibers And Polymers. 2005, 6(4): 322-331.
Liu R., Kwok Y.L., Li Y., Lao T.T.H., Zhang X. and Dai X.Q., Objective evaluation of skin pressure distribution of graduated elastic compression stockings, Dermatologic Surgery. 2005, 31(6): 615-624.
Liu R., Kwok Y.L., Lao T.T.H. and Zhang X., The effects of graduated compression stockings (GCSs) on cutaneous surface pressure along the path of main superficial veins of lower limbs, Wounds - A Compendium of Clinical Research and Practice. 2006, 18: 150-157.
Ma R.M., Lao T.T.H., Lu Y.F., Du M.Y., Ma C.L., Liao S.J., Xiao H., Yang M.H. and Zhang L., Relationship between leg length and gestational diabetes mellitus in Chinese pregnant women, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.
Tse K.Y., Ho L.F. and Lao T.T.H., The impact of maternal HBsAg carrier status on pregnancy outcomes: A case-control study, Journal of Hepatology. 2005, 43: 771-775.


Researcher : Lau ETK

List of Research Outputs

Lau E.T.K., Kwok K.Y., Leung K.Y. and Tang M.H.Y., Automating non-invasive prenatal diagnosis of HB Bart's disease on immunofluorescence staining of fetal erythrocytes in maternal blood, The 13th Conference on Prenatal Diagnosis and Therapy, May 28-31, 2006, Kyoto, Japan. 2006.


Researcher : Lau EYL

List of Research Outputs

Chan C.C.W., Ng E.H.Y., Tang F.O.S., Yeung W.S.B., Lau E.Y.L. and Ho P.C., A prospective, randomized, double-blind study to compare two doses of recombinant human chorionic gonadotropin in inducing final oocyte maturity and the hormonal profile during the luteal phase, The Journal of Clinical Endocrinology and Metabolism. 2005, 90(7): 3933-3938.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Content And 4977 bp Deletion In Unselected And Unfertilized Oocytes, Molecular Human Reproduction. 2006, 11: 843-846.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Deletion In Granulosa And Cumulus Oophorus Cells, Fertility and Sterility. 2006, 85: 780-782.


Researcher : Lau WNT

List of Research Outputs

Chan C.C.W., Lau W.N.T., Chiu S.P., Chen L.C., Choi W.K. and Tang G.W.K., A pilot study on the effects of a Chinese herbal preparation on menopausal symptoms, Gynecological Endocrinology. 2006, 22(2): 70-73.
Chan C.C.W., Tang F.O.S., Lau W.N.T. and Tang G.W.K., Bone turnover in young hypoestrogenic women on hormonal therapy, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 124: 204-206.
Li I.C.F., Lau W.N.T. and Pun T.C., Umbilical wound hernia following a 5-mm threaded cannula wound with primary repair stitch in situ, Acta Obstetricia et Gynecologica Scandinavica. 2005, 84: 1125-1126.


Researcher : Lee CKF

Project Title:Identification of implantation-related genes in mouse uterus: an genomic approach
Investigator(s):Lee CKF, Yeung WSB, Yang ZM
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To identify genes that are more directly related to implantation by using LCM. mRNA from specific endometrial cells around the implantation sites of mouse at Day 5.5 of pregnancy will be compared with endometrial cells at the inter-implantation site of the deciduas.


Project Title:The molecular interaction between human oviductal embryotrophic factor-3 and embryo
Investigator(s):Lee CKF
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
N/A


Project Title:The molecular interaction between human oviductal embryotrophic factor-3 and embryo
Investigator(s):Lee CKF, Luk JMC, Yeung WSB
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2005
Abstract:
To compare the fecundity of normal and C3-deficient mice; to determine the role of complement activation in the embryotrophic activity of C3; to determine the machinery for the conversion of C3/C3 to iC3b in the oviduct cell coculture system; to define the fragment of iC3b responsible for the embryotrophic activity of the molecule.


Project Title:Cloning and functional characterization of demilune cell and parotid protein (Dcpp) gene promoter
Investigator(s):Lee CKF, Yeung WSB
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:01/2006
Abstract:
The objectives of my proposal are (1) to isolate the promoter region of the mouse demilune cell and parotid protein (Dcpp) gene promoter, and (2) to characterise the hormonal regulation of the isolated promoter regions by transfection studies. Demiline cell and parotid protein (acc. no.: NM_019910, Dcpp, as named as p20) was first isolated from both sublingual demilune cells and parotid intercalated duct cells in mouse salivary glands [1] and shared very high homology (99% aa identity) with the common salivary gland 1 (acc. no.: S76879, CSP1) protein. The gene coding for Dcpp was located on chromosome 17A3.3 and encodes a 150aa polypeptide. Recently, we used suppression subtractive hybridization (SSH) to compare the gene expression patterns of oviduct containing-developing embryo, with the contralateral oviduct containing-oocyte to isolate genes that are up-regulated in the presence of developing embryo [2, 3]. One gene homologous to Dcpp was found to be highly upregulated in the oviductal epithelium containing developing embryo, and is under steroid hormone control in ovariectomized mouse model in vivo [4]. Bioinformatics search identified a human CSP1 homolog (acc. no.: NM_145252). In order to identify the region and understand the hormonal regulation of this gene in the oviduct during pre-implantation period, we propose to clone the Dcpp promoter and study its regulation in vitro. References: [1] Bekhor I et al. (1994) cDNA cloning, sequencing and in situ localization of a transcript specific to both sublingual demilune cells and parotid intercalated duct cells in mouse salivary glands. Arch Oral Biol 39, 1011-1022. [2] Lee KF et al. (2002) Early developing embryos affect the gene expression patterns in the mouse oviduct. Biochem. Biophys. Res. Commun. 292, 564-570. [3] Lee KF et al. (2005) Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct. J. Cell. Biochem. 95, 740-749. [4] Lee KF et al. (2005) Demilune Cell Parotid Protein (Dcpp) from Murine Oviductal Epithelium Stimulates Pre-implantation Embryo Development. Endocrinology, in revision.


Project Title:Functional characterization of VAD1.3, a novel acrosome-specific protein by conditional tissue-specific gene inactivation
Investigator(s):Lee CKF
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2006
Abstract:
N/A


Project Title:Functional characterization of VAD1.3, a novel acrosome-specific protein by conditional tissue-specific gene inactivation
Investigator(s):Lee CKF, Cheah KSE, Yeung WSB, Luk JMC, Lee PY
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2006
Abstract:
To generate VAD1.3 knockout targeting construct; to generate VAD1.3 knockout mice; to produce antibodies against mouse VAD1.3; to analysis the VAD1.3 knockout mice.


List of Research Outputs

Ault K., Neff T., Kelly K., Yeung W.S.B., Tsao S.W. and Lee C.K.F., Chemokine and cytokine gene expression in immortalized human oviduct epithelial cell infected with chlamydia trachomatis, Annual Scientific Meeting and Symposium of the Infectious Disease Society for Obstetrics and Gynecology (IDSOG), South Carolina, USA, August 18-20, 2005.
Chiu C.N., Chung M.K., Lee C.K.F., Ho P.C. and Yeung W.S.B., Adrenomedullin effects on sperm functions. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Chiu C.N., Chung M.K., Tsang H.Y., Koistinen R., Koistinen H., Seppala M., Lee C.K.F. and Yeung W.S.B., Glycodelin-S in human seminal plasma reduces cholesterol efflux and inhibits capacitation of spermatozoa, The Journal of Biological Chemistry. 2005, 280(27): 25580-25589.
Chung M.K., Chiu C.N., Lee C.K.F., Ho P.C. and Yeung W.S.B., A role for human sperm surface fucosyltransferase in glycodelin binding., International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006, P.10.
Chung M.K., Chiu C.N., Lee C.K.F., Yeung W.S.B. and Ho P.C., Biological and biochemical characterization of the glycodelin isoform from human cumulus cell matrix. P5.38, Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005.
Fung W.T., Sze K.H., Lee C.K.F. and Shaw P.C., Functional studies of the small subunit of EcoHK311 DNA methyltransferase, Biological Chemistry. 2006, 387(5): 507-513.
Lee C.K.F., Xu J., Lee C.Y.L. and Yeung W.S.B., Demilune cell and parotid protein from murine oviductal epithelium stimulates preimplantation embryo development, Endocrinology. 2006, 147(1): 79-87.
Lee C.K.F. and Yeung W.S.B., Gamete/Embryo-Oviduct Interactions: Implications on in vitro culture. , British Andrology Society Annual Meeting. 17-18 Nov, Sheffield, UK.. 2005, P.10.
Lee C.K.F., Leung K.Y., Chung M.K., Lee C.Y.L., Chow J.F.C. and Yeung W.S.B., Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct, Journal of Cellular Biochemistry. 2005, 95: 740-749.
Lee C.K.F., Cheung W.K.L., Chung M.K., Chiu C.N., Yeung W.S.B. and Ng E.H.Y., Proteomic profiling of the uterine flushing from natural and stimulated cycles during peri-implantation period., Society for the Study of Reproduction -38th Annual Meeting. 24-27 Jul, Quebec, Canada. . 2005.
Lee P.Y., Tong M.K., Leung P.L., Chan V.W.M., Leung S.Y.L., Tam P.C., Chan K.W., Lee C.K.F., Yeung W.S.B. and Luk J.M.C., Kidney claudin-19: Localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease, FEBS Letters. 2006, 580(3): 923-31.
Ling L., Lee C.Y.L., Lee C.K.F., Tsao G.S.W., Yeung W.S.B. and Kan F.W.K., Expression of human oviductin in an immortalized human oviductal cell line, Fertility and Sterility. 2005, 84 Supplement 2: 1095-1103.
Ling L., Lee C.Y.L., Lee C.K.F., Yeung W.S.B. and Kan F.W.K., Study of gene and protein expression of oviductin in an immortalized human oviductal cell line, 38th Annual Meeting, Society for The Study of Reproduction, Quebec, Canada, July 24-27, 2005.
Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyper-stimulated cycle during in vitro fertilization treatment. , In: Liu Y, Lee KF, Ng EHY, Ho PC , Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005, P5.39.
Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyperstimulated cycle during in vitro fertilization treatment, 38th Annual Meeting, Society for The Study of Reproduction, Quebec, Canada, July 24-27, 2005.
Siu S.O., Cox D., Lee C.K.F., Yeung W.S.B. and Chu I.K., Characterization of N-linked Glycosylation sites and glycams of glycodelins by means of tandem mass spectrometry, 54th American Society for Mass Spectrometry Conference, Seattle, USA. 2006.
Siu S.O., Cox D., Lee C.K.F., Yeung W.S.B. and Chu I.K., Characterization of N-linked glycosylation sites and glycans of glycodelins by means of tandem mass spectrometry , Poster Presentation in Proceedings of 54th ASMS Conference on Mass Spectrometry and Allied Topies, Seattle, Washington State, U.S.A., May 28 - June 1, 2006 . 2006, Poster No. 394.
Siu S.O., Cox O., Lee C.K.F., Yeung W.S.B. and Chu I.K., Characterization of N-linked glycosylation sites and glycans of glycodelins by means of tandem mass spectrometry, Poster Presentation in 13rd Symposium on Chemistry Postgraduate Research in Hong Kong . 2006, A19.
Yeung W.S.B., Lee C.Y.L. and Lee C.K.F., Coculture versus sequential culture in assisted reproduction., In: Yeung WS, Lee YL, Lee KF , The XIXth Asian and Oceanic Congress of Obstetrics and Gynaecology. S4C-3. 1-5 Oct, Seoul, Korea.. 2005.
Yeung W.S.B., Lee C.K.F. and Lee C.Y.L., Complement protein-3: an oviductal paracrine factor regulating embryo development, 12th World Congress of Gynecological Endocrinology, Florence, Italy, March 2-5, 2006. 2006.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Glycodelin: A molecule with multi-functions on spermatozoa. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Yeung W.S.B., Lee C.K.F. and Lee C.Y.L., Oviductal embryotrophic factors, 5th International Conference on Reproductive Endocrinology, 9-12 September 2005 Beijing, China.. 2005.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Roles of glycodelin in modulating sperm function, Molecular and Cellular Endocrinology. 2006, 250: 149-56.


Researcher : Lee CP

List of Research Outputs

Chan C.P., Leung W.C., Leung K.Y., Pun T.C. and Lee C.P., Confirmation of first trimester fetal abnormalities at termination of pregnancy, The 15th World Congress on Ultrasound in Obstetrics and Gynecology (ISUOG 2005), September 25-29, 2005, Vancouver, Canada. 2005.
Chen M., Leung K.Y., Lee C.P., Tang M.H.Y. and Ho P.C., Placental volume measured by three-dimensional ultrasound in the prediction of fetal ao-thalassemia: a preliminary report, Ultrasound Obstetrics and Gynecology. 2006, 28: 166-172.
Chen M., Chan G.S., Lee C.P. and Tang M.H.Y., Sonographic features of lethal multiple pterygium syndrome at 14 weeks, Prenatal Diagnosis. 2005, 25: 475-478.
Hui P.W., Lee C.P., Tang M.H.Y. and Ho P.C., Nuchal translucency in pregnancies conceived after assisted reproduction technology, Current Opinion in Obstetrics and Gynecology. 2006, 18: 319-324.
Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., Amniotic fluid volume at 41 weeks and infant outcome, Journal of Reproductive Medicine. 2006, 51: 484-488.
Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., The use of fetal Doppler cerebroplacental blood flow and amniotic fluid volume measurement in the surveillance of postdated pregnancies, In: Lam H, Leung WC, Lee CP, Lao TT, Acta Obstetricia et Gynecologica Scandinavica. 2005, 84: 844-848.
Leung K.Y., Chan K.L., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for severe twin-twin transfusion syndrome: A case report, Journal of Paediatrics, Obstetrics and Gynaecology. 2005, Sep/Oct: 202-203.
Leung K.Y., Chan K.K.L., Ngai C.S.W., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W., Chan B.C., Leung W.C., Lee C.P. and Tang M.H.Y., Three-dimensional extended imaging: a new display modality for three-dimensional ultrasound examination, Ultrasound Obstetrics and Gynecology. 2005, 26: 244-251.
Tam K.F., Lee C.P. and Pun T.C., Mild postnatal anemia: Is it a problem?x, American Journal of Perinatology. 2005, 22(7): 345-349.
Tse K.Y., Leung W.C., Leung K.Y., Lee C.P., Ng L.K.L., Lau E.T., Chan V.N.Y. and Tang M.H.Y., Full karyotyping, rapid aneuploidy diagnosis or both when invasive prenatal testing is performed for diagnosis of thalassaemia?, Molecular Human Reproduction. 2006, 12(1): 55-59.


Researcher : Lee CYL

Project Title:Effects of C3 deficiency on preimplantation embryo development and implantation potential
Investigator(s):Lee CYL, Yeung WSB, Lee CKF
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2005
Abstract:
Coculture of embryo with somatic cells is one of the methods to promote embryo development in vitro. We have shown previously that human oviductal epithelial cells secrete embryotrophic glycoproteins that stimulate the growth of mouse preimplantation embryos in vitro (1, 2). Embryotrophic factor-3 (ETF-3) is one of these factors. ETF-3. It enhances the development of mouse blastocysts resulting in blastocysts with more trophectoderm (TE) cells, larger diameter, higher hatching rate and hatched blastocyst with higher attachment potential and larger trophoblast outgrowth (3, 4). It also affects the expression of a number of genes (NKA-beta 1, PAR-2, cullin-1, ezrin, HSP-70, eIF-2 beta) in the treated blastocysts. Our laboratory recently identified ETF-3 to be complement 3. Its derivative, iC3b increases the preimplantation mouse embryo development in terms of higher blastulation rate, higher hatching rate and larger blastocyst size (5). The objective of this project is to study the effect of C3 knockout in the mouse on the development of the embryo. Similar study in human is difficult, if not impossible. 1. L. P. Liu, S. T. Chan, P. C. Ho, W. S. Yeung, Hum. Reprod 10, 2781 (1995). 2. L. P. Liu, S. T. Chan, P. C. Ho, W. S. Yeung, Hum. Reprod 13, 1613 (1998). 3. J. S. Xu, T. M. Cheung, S. T. Chan, P. C. Ho, W. S. Yeung, Biol Reprod 65, 1481 (2001). 4. Y. L. Lee et al., Biol Reprod 68, 375 (2003). 5. Y. L. Lee et al., J. Biol. Chem. (2003).


List of Research Outputs

Lee C.K.F., Xu J., Lee C.Y.L. and Yeung W.S.B., Demilune cell and parotid protein from murine oviductal epithelium stimulates preimplantation embryo development, Endocrinology. 2006, 147(1): 79-87.
Lee C.K.F., Leung K.Y., Chung M.K., Lee C.Y.L., Chow J.F.C. and Yeung W.S.B., Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct, Journal of Cellular Biochemistry. 2005, 95: 740-749.
Ling L., Lee C.Y.L., Lee C.K.F., Tsao G.S.W., Yeung W.S.B. and Kan F.W.K., Expression of human oviductin in an immortalized human oviductal cell line, Fertility and Sterility. 2005, 84 Supplement 2: 1095-1103.
Ling L., Lee C.Y.L., Lee C.K.F., Yeung W.S.B. and Kan F.W.K., Study of gene and protein expression of oviductin in an immortalized human oviductal cell line, 38th Annual Meeting, Society for The Study of Reproduction, Quebec, Canada, July 24-27, 2005.
Yeung W.S.B., Lee C.Y.L. and Lee C.K.F., Coculture versus sequential culture in assisted reproduction., In: Yeung WS, Lee YL, Lee KF , The XIXth Asian and Oceanic Congress of Obstetrics and Gynaecology. S4C-3. 1-5 Oct, Seoul, Korea.. 2005.
Yeung W.S.B., Lee C.K.F. and Lee C.Y.L., Complement protein-3: an oviductal paracrine factor regulating embryo development, 12th World Congress of Gynecological Endocrinology, Florence, Italy, March 2-5, 2006. 2006.
Yeung W.S.B., Lee C.K.F. and Lee C.Y.L., Oviductal embryotrophic factors, 5th International Conference on Reproductive Endocrinology, 9-12 September 2005 Beijing, China.. 2005.


Researcher : Lee WH

List of Research Outputs

Tang F.O.S., Ong C.Y.T., Tse K.Y., Ng E.H.Y., Lee W.H. and Ho P.C., A randomized trial to compare the use of sublingual misoprostol with or without an additional 1 week course for the management of first trimester silent miscarriage, Human Reproduction. 2006, 21(1): 189-192.


Researcher : Leung CY

List of Research Outputs

Liu S., Chan Y.K., Leung C.Y., Law H.K.W., Leung T.W. and Ngan H.Y.S., Enhancement of the radiosensitivity of cervical cancer cells by overexpressing p73a, Molecular Cancer Therapeutics. USA, American Association of Cancer Research, 2006, 5: 1209-1215.


Researcher : Leung KY

List of Research Outputs

Chan C.P., Leung W.C., Leung K.Y., Pun T.C. and Lee C.P., Confirmation of first trimester fetal abnormalities at termination of pregnancy, The 15th World Congress on Ultrasound in Obstetrics and Gynecology (ISUOG 2005), September 25-29, 2005, Vancouver, Canada. 2005.
Chen M., Leung K.Y., Lee C.P., Tang M.H.Y. and Ho P.C., Placental volume measured by three-dimensional ultrasound in the prediction of fetal ao-thalassemia: a preliminary report, Ultrasound Obstetrics and Gynecology. 2006, 28: 166-172.
Lau E.T.K., Kwok K.Y., Leung K.Y. and Tang M.H.Y., Automating non-invasive prenatal diagnosis of HB Bart's disease on immunofluorescence staining of fetal erythrocytes in maternal blood, The 13th Conference on Prenatal Diagnosis and Therapy, May 28-31, 2006, Kyoto, Japan. 2006.
Lee C.K.F., Leung K.Y., Chung M.K., Lee C.Y.L., Chow J.F.C. and Yeung W.S.B., Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct, Journal of Cellular Biochemistry. 2005, 95: 740-749.
Leung K.Y., Chan K.L., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for severe twin-twin transfusion syndrome: A case report, Journal of Paediatrics, Obstetrics and Gynaecology. 2005, Sep/Oct: 202-203.
Leung K.Y., Chan K.K.L., Ngai C.S.W., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Endoscopic laser therapy for twin-twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W. and Tang M.H.Y., How three-dimensional ultrasound help in the prenatal diagnosis of fetal anomalies?, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W., Chan B.C., Leung W.C., Lee C.P. and Tang M.H.Y., Three-dimensional extended imaging: a new display modality for three-dimensional ultrasound examination, Ultrasound Obstetrics and Gynecology. 2005, 26: 244-251.
Leung K.Y., Ling M. and Tang G.W.K., Use of hormone replacement therapy in the Hong Kong public health sector after the women's health initiative trial, Maturitas. 2005, 52: 277-285.
Tse K.Y., Leung W.C., Leung K.Y., Lee C.P., Ng L.K.L., Lau E.T., Chan V.N.Y. and Tang M.H.Y., Full karyotyping, rapid aneuploidy diagnosis or both when invasive prenatal testing is performed for diagnosis of thalassaemia?, Molecular Human Reproduction. 2006, 12(1): 55-59.


Researcher : Leung TW

Project Title:The role of p73 in radiation response in cervical cancer
Investigator(s):Leung TW, Ngan HYS, Cheung ANY
Department:Obstetrics & Gynaecology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:10/2003
Completion Date:09/2005
Abstract:
To study expression level, allelic loss, and promoter methylation status of p73 in radiosensitive and radioresistant cervical cancer; to study the association between the expression of p73 and the presence of HPV 16-E6 and -E7 in radiosensitive and radioresistant cervical cancer; to investigate the effect of HPV16-E6 and/or-E7 on radiosensitivity of the cancer cells independent and dependent of p73; to explore irradiation response of the cervical cancer cell independent and dependent of p73.


Project Title:The role of p73 in radiation response in cervical cancer
Investigator(s):Leung TW, Ngan HYS, Cheung ANY
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:10/2003
Completion Date:09/2005
Abstract:
N/A


List of Research Outputs

Liu S., Chan Y.K., Leung C.Y., Law H.K.W., Leung T.W. and Ngan H.Y.S., Enhancement of the radiosensitivity of cervical cancer cells by overexpressing p73a, Molecular Cancer Therapeutics. USA, American Association of Cancer Research, 2006, 5: 1209-1215.
Tiwari A.F.Y., Leung W.C., Leung T.W., Humphreys J., Parker B. and Ho P.C., A randomised trial of empowerment training for Chinese abused pregnant women in Hong Kong, Frontiers in Biomedical Research: Achieving Excellence through Multi-disciplinary Research, Faculty of Medicine, The University of Hong Kong. Hong Kong, 2005, p.81.


Researcher : Leung WC

List of Research Outputs

Chan C.P., Leung W.C., Leung K.Y., Pun T.C. and Lee C.P., Confirmation of first trimester fetal abnormalities at termination of pregnancy, The 15th World Congress on Ultrasound in Obstetrics and Gynecology (ISUOG 2005), September 25-29, 2005, Vancouver, Canada. 2005.
Kwok R.P.W., Law Y.K., Li K.E., Ng Y.C., Cheung M.H., Fung V.K.P., Kwok K.T.T., Tong J.M.K., Yen P.F. and Leung W.C., Prevalence of workplace violence against nurses in Hong Kong, Hong Kong Medical Journal. 2006, 12(1): 6-9.
Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., Amniotic fluid volume at 41 weeks and infant outcome, Journal of Reproductive Medicine. 2006, 51: 484-488.
Lam H.S.W., Leung W.C., Lee C.P. and Lao T.T.H., The use of fetal Doppler cerebroplacental blood flow and amniotic fluid volume measurement in the surveillance of postdated pregnancies, In: Lam H, Leung WC, Lee CP, Lao TT, Acta Obstetricia et Gynecologica Scandinavica. 2005, 84: 844-848.
Lao T.T.H., Chan C.P., Leung W.C. and Ho L.F., Maternal age and prevalence of gestational diabetes mellitus, Diabetes Care. 2006, 29: 948-949.
Leung K.Y., Chan K.L., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for severe twin-twin transfusion syndrome: A case report, Journal of Paediatrics, Obstetrics and Gynaecology. 2005, Sep/Oct: 202-203.
Leung K.Y., Chan K.K.L., Ngai C.S.W., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W., Chan B.C., Leung W.C., Lee C.P. and Tang M.H.Y., Three-dimensional extended imaging: a new display modality for three-dimensional ultrasound examination, Ultrasound Obstetrics and Gynecology. 2005, 26: 244-251.
Leung W.C., Application of rapid aneuploidy screening for prenatal diagnosis, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.
Leung W.C., Lam H.S.W., Lao T.T.H. and Tang M.H.Y., Biochemical hypothyroidism-A new finding in morror syndrome?, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 125(2): 269-271.
Leung W.C. and Lao T.T.H., Rapid aneuploidy testing, traditional karyotyping, or both?, In: Leung WC, Lao TT, The Lancet. 2005, 366(9480): 97-98.
Ting J.Y., Lam B.C.C., Ngai C.S.W., Leung W.C. and Chau K.L., Splenic rupture in a premature neonate, Hong Kong Medical Journal. 2006, 12(1): 68-70.
Tiwari A.F.Y., Leung W.C., Leung T.W., Humphreys J., Parker B. and Ho P.C., A randomised controlled trial of empowerment training for Chinese abused pregnant women in Hong Kong, British Journal of Obstetrics & Gynaecology. 2005, 112: 1249-56.
Tiwari A.F.Y., Leung W.C., Leung T.W., Humphreys J., Parker B. and Ho P.C., A randomised trial of empowerment training for Chinese abused pregnant women in Hong Kong, Frontiers in Biomedical Research: Achieving Excellence through Multi-disciplinary Research, Faculty of Medicine, The University of Hong Kong. Hong Kong, 2005, p.81.
Tiwari A.F.Y., Chan E.K.L., Leung W.C., Fong D.Y.T. and Ho P.C., The effectiveness of the Chinese version of the Abuse Assessment Screen to identify abused women, the Hospital Authority Convention 2006: From Policy to Practice, organized by the Hospital Authority, Hong Kong. 2006.
Tiwari A.F.Y., Chan E.K.L., Leung W.C., Fong D.Y.T. and Ho P.C., The prevalence of intimate partner violence among pregnant women, the Hospital Authority Convention 2006: From Policy to Practice, organized by the Hospital Authority, Hong Kong. 2006.
Tse K.Y., Leung W.C., Leung K.Y., Lee C.P., Ng L.K.L., Lau E.T., Chan V.N.Y. and Tang M.H.Y., Full karyotyping, rapid aneuploidy diagnosis or both when invasive prenatal testing is performed for diagnosis of thalassaemia?, Molecular Human Reproduction. 2006, 12(1): 55-59.


Researcher : Li ICF

List of Research Outputs

Li I.C.F., Lau W.N.T. and Pun T.C., Umbilical wound hernia following a 5-mm threaded cannula wound with primary repair stitch in situ, Acta Obstetricia et Gynecologica Scandinavica. 2005, 84: 1125-1126.


Researcher : Liu S

List of Research Outputs

Liu S., Chan Y.K., Leung C.Y., Law H.K.W., Leung T.W. and Ngan H.Y.S., Enhancement of the radiosensitivity of cervical cancer cells by overexpressing p73a, Molecular Cancer Therapeutics. USA, American Association of Cancer Research, 2006, 5: 1209-1215.


Researcher : Liu VWS

Project Title:Identification of differentially expressed genes in cervical cancer cells under physiological stresses
Investigator(s):Liu VWS, Ngan HYS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:01/2005
Completion Date:01/2006
Abstract:
Introduction Cervical carcinoma is the most common cancer of the female genital tract and is still a major cause of morbidity and mortality in women of Hong Kong. Human papillomaviruses (HPVs) have been found in nearly all cases of cervical cancer, and it has been well established to be an important aetiological factor for the development of the cancer (zur Hausen, 2002). Although HPV infection is generally believed to be the initiating factor for cervical cancer development, the development of fully invasive tumour must involve other factors, such as genetic alterations in oncogenes and tumour suppressor genes, epigenetic alterations in cancer-related genes and changes in gene expression profile, to promote tumour formations. As tumour grows, because of hypovascularization, many cancer cells are often exposed to adverse conditions such as glucose deprivation, insufficient supply of oxygen and other nutrients (Brown and Giaccia, 1998; Dang and Semenza, 1999; Hockel et al, 1999; Semenza, 2003; Giaccia et al, 2004). In this situation, the tumour may become quiescent. However, aggressive tumour may acquire an adaptation to adverse conditions to further growth and development. This adaptative mechanism may involve the up-regulation and/or down-regulation of many stress-responsive genes. Briefly, from others’ work, a number of genes have been shown to respond to stress stimulation and made the cancer cells more resistant to adverse conditions. For examples, pancreatic cell lines but not liver cell lines have been shown to tolerate to nutrient deprivation (Izuishi et al, 2000). Increased expression of hypoxia-inducible factor-1 alpha, and anaerobic metabolism-associated genes including glucose transporter-1 and aldoase A, may contribute to the survival and proliferation of pancreatic cancer cells in hypoxia and glucose deprivation (Akakura et al, 2001). Kato et al. (2002) has demonstrated that elevated expression of AMP-activated protein kinase (AMPK) may directly contribute to tolerance to nutrient deprivation in cancer cells. AMPK antisense RNA expression in pancreatic cancer cells inhibited tumour growth in nude mice. AMPK has been proposed to be potentially useful as a new target for cancer therapy to suppress tumour growth and invasion (Kato et al, 2002). Using comparative genome hybridization, our own work has also demonstrated the occurrence of gene amplification and over-expression of the AMPK alpha 1 subunit in cervical cancer (Huang et al., in press, Huang et al., submitted). It is anticipated that much greater number of stress-responsive genes are awaiting to be discovered using the genome-wide analytical scanning such as cDNA microarray. Objectives: (1) To determine, by in vitro cell culture, the degree of tolerance of cervical cancer cells to stress (2) To identify, using microarray technology, differentially expressed genes in cervical cancer cells under physiological stresses such as hypoxia or nutrient deprivation


List of Research Outputs

Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Content And 4977 bp Deletion In Unselected And Unfertilized Oocytes, Molecular Human Reproduction. 2006, 11: 843-846.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Deletion In Granulosa And Cumulus Oophorus Cells, Fertility and Sterility. 2006, 85: 780-782.
Huang F.Y., Chiu P.M., Tam K.F., Kwok K.Y., Lau E.T., Tang M.H., Ng T.Y., Liu V.W.S., Cheung A.N.Y. and Ngan H.Y.S., Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer, Gynecologic Oncology. 2006, 103(1): 219-25.
Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes In The Mitochondrial Content Of Cervical Cancer Cells Under Stress, The 3rd Asain Society for Mitochondrial Research and Medicine December 14-17, 2005, Tokyo, Japan. 2005.
Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes in mitochondrial content of cervical cancer cells under stress, The 3rd Conference of the Asian Society for Mitochondrial Research and Medcine , December 14-17, 2005, Tokyo, Japan. 2005.
Wang Y., Liu V.W.S., Tsang P.C.K., Chiu P.M., Cheung A.N.Y., Khoo U.S., Nagley P. and Ngan H.Y.S., Microsatellite instability in mitochondrial genome of common female cancers, International Journal of Gynecological Cancer. 2006, Suppl 1: 259-266.
Wang Y., Liu V.W.S., Xue W., Tsang P.C.K., Cheung A.N.Y. and Ngan H.Y.S., The Increase Of Mitochondrial Dna Content In Endometrial Adenocarcinoma Cells: A Quantitative Study Using Laser-captured Microdissected Tissues, Gynecologic Oncology. 2005, 98: 104-110.


Researcher : Liu Y

List of Research Outputs

Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyper-stimulated cycle during in vitro fertilization treatment. , In: Liu Y, Lee KF, Ng EHY, Ho PC , Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005, P5.39.
Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyperstimulated cycle during in vitro fertilization treatment, 38th Annual Meeting, Society for The Study of Reproduction, Quebec, Canada, July 24-27, 2005.


Researcher : Makkar G

List of Research Outputs

Makkar G., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Reduced expression of interleukin-11 and interleukin-6 in the peri-implantation endometrium of excessive ovarian responders during in-vitro fertilization treatment, Journal of Clinical Endocrinology and Metabolism. 2006, 91(8): 3181-3188.


Researcher : Ng EHY

Project Title:Single or multiple biopsies for retrieval of spermatozoa from the testis?
Investigator(s):Ng EHY, Tam PC, Chau MT, O WS, Ho PC, Yeung WSB
Department:Obstetrics & Gynaecology
Source(s) of Funding:Other Funding Scheme
Start Date:03/1999
Abstract:
To compare the recovery rate of testicular spermatozoa after single vs. multiple open testicular biopsies; to compare the testicular function after single vs. multiple open testicular biopsies.


Project Title:To study the endometrial receptivity before and after removing intramural fibroids
Investigator(s):Ng EHY, Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Other Funding Scheme
Start Date:01/2000
Abstract:
To compare endometrial receptivity in patients having intramural fibroids before and after myomectomy.


Project Title:A randomized comparison of side effects of two vaginal progesterone preparations used for luteal support in assisted reproduction cycles using pituitary down regulation
Investigator(s):Ng EHY, Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Other Funding Scheme
Start Date:07/2000
Abstract:
To compare the side effects of two vaginal progesterone preparations used for luteal support in assisted reproduction cycles using pituitary down regulation.


Project Title:The value of increasing starting dose of gonadotrophin during ovarian stimulation in women with <= 6 antral follicles prior to stimulation
Investigator(s):Ng EHY, Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Other Funding Scheme
Start Date:08/2000
Abstract:
To determine the value of increasing starting dose of gonadotrophin during ovarian stimulation in women with <= 6 antral follicles prior to stimulation.


Project Title:The prevalence of polycystic ovaries in Chinese women with history of gestational diabetes or impaired glucose tolerance
Investigator(s):Ng EHY, Ho PC, Chan CW
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Completion Date:10/2005
Abstract:
To define the prevalence of polycystic ovaries in Chinese women with history of gestational diabetes or impaired glucose tolerance in our local population, as well as their associated clinical and biochemical features.


Project Title:Role of antral follicle count and ovarian stromal blood flow in predicting pregnancy of normal healthy women attempting to get pregnant for the first time
Investigator(s):Ng EHY
Department:Obstetrics & Gynaecology
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2005
Completion Date:06/2006
Abstract:
N/A


Project Title:Comparison of the prevalence of polycystic ovary only and polycystic ovary syndrome between two different ethnic populations
Investigator(s):Ng EHY, Chan CW, Yeung SB, Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:09/2005
Abstract:
The objective of this study is To define the prevalence of polycystic ovary only and polycystic ovary syndrome and its associated hormonal and biochemical profiles in Chinese and Indian populations.


List of Research Outputs

Ajonuma L.C., Ng E.H.Y., Chan L.N., Chow P.H., Kung L.S., Cheung A.N.Y., Ho L.S., Briton-Jones C., Lok I.H., Haines C.J. and Chan H.C., Ultrastructural characterization of whole hydrosalpinx from infertile Chinese women, Cell Biology International. 2005, 29: 849-856.
Chan C.C.W., Ng E.H.Y., Tang F.O.S., Yeung W.S.B., Lau E.Y.L. and Ho P.C., A prospective, randomized, double-blind study to compare two doses of recombinant human chorionic gonadotropin in inducing final oocyte maturity and the hormonal profile during the luteal phase, The Journal of Clinical Endocrinology and Metabolism. 2005, 90(7): 3933-3938.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Circadian changes in uterine artery and ovarian stromal blood flow after pituitary down-regulation, Journal of The Society for Gynecology Investigation. 2005, 12(6): 452-455.
Chan C.C.W., Koo M.W.L., Ng E.H.Y., Tang F.O.S., Yeung W.S.B. and Ho P.C., Effects of Chinese green tea on weight, and hormonal and biochemical profiles in obese patients with polycystic ovary syndrome - A randomized placebo-controlled trial, Journal of The Society for Gynecology Investigation. 2006, 13(1): 64-68.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Endometrial, subendometrial, and ovarian stromal blood flow in patients treated with two different doses of recombinant human chorionic gonadotropin , Fertility and Sterility. 2006, 85(2): 520-522.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Content And 4977 bp Deletion In Unselected And Unfertilized Oocytes, Molecular Human Reproduction. 2006, 11: 843-846.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Deletion In Granulosa And Cumulus Oophorus Cells, Fertility and Sterility. 2006, 85: 780-782.
Chan C.C.W., Ng E.H.Y. and Ho P.C., Obesity and reproduction, Journal of Paediatrics, Obstetrics and Gynaecology. 2006, 32(3): 127-131.
Chan C.H.Y., Chan C.L.W., Ng S.M., Ng E.H.Y. and Ho P.C., Body-mind-spirit intervention for IVF women, Journal of Assisted Reproduction and Genetics. 2005, 22(11&12): 419-427.
Chan C.H.Y., Ng E.H.Y., Chan C.L.W., Ho P.C. and Chan H.Y., Effectiveness of psychosocial group intervention for reducing anxiety in women undergoing in vitro fertilization: A randomized controlled study, Fertility and Sterility. 2006, 85(2): 339-346.
Ho P.C., Chan C.C.W., Tang F.O.S. and Ng E.H.Y., Emergency contraception, Gynaecology Forum. 2005, 10(4): 16-18.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, Prenatal Diagnosis. 2006, 26: 510-513.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, The 13th International Conference on Prenatal Diagnosis and Therapy, Kyoto, Japan, 28 - 31 May. 2006.
Kan A., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Perifollicular vascularity in poor ovarian responders during IVF, Human Reproduction. 2006, 21(6): 1539-1544.
Lee C.K.F., Cheung W.K.L., Chung M.K., Chiu C.N., Yeung W.S.B. and Ng E.H.Y., Proteomic profiling of the uterine flushing from natural and stimulated cycles during peri-implantation period., Society for the Study of Reproduction -38th Annual Meeting. 24-27 Jul, Quebec, Canada. . 2005.
Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyper-stimulated cycle during in vitro fertilization treatment. , In: Liu Y, Lee KF, Ng EHY, Ho PC , Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005, P5.39.
Liu Y., Lee C.K.F., Ng E.H.Y. and Ho P.C., Expression profiles of endometria from women with natural, moderate and hyperstimulated cycle during in vitro fertilization treatment, 38th Annual Meeting, Society for The Study of Reproduction, Quebec, Canada, July 24-27, 2005.
Makkar G., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Reduced expression of interleukin-11 and interleukin-6 in the peri-implantation endometrium of excessive ovarian responders during in-vitro fertilization treatment, Journal of Clinical Endocrinology and Metabolism. 2006, 91(8): 3181-3188.
Ng E.H.Y., Advances in ultrasound in ART, Second Meeting of Global Chinese Conference on Reproductive Medicine, The Global Chinese Association of Reproductive Medicine, Beijing, China, 4-6 August, 2005.
Ng E.H.Y., Chan C.C.W. and Ho P.C., Are there differences in ultrasound parameters between Chinese women with polycystic ovaries only and with polycystic ovary syndrome?, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 125: 92-98.
Ng E.H.Y., Chan C.C.W., Tang F.O.S. and Ho P.C., Comparison of endometrial and subendometrial blood flows among patients with and without hydrosalpinx shown on scanning during in vitro fertilization treatment, Fertility and Sterility. 2006, 85(2): 333-338.
Ng E.H.Y., Chan C.C.W., Yeung W.S.B. and Ho P.C., Comparison of ovarian stromal flow between fertile women with normal ovaries and infertile women with polycystic ovary syndrome, The 6th International Scientific Meeting, Cairo, Egypt, September 27-30, 2005.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Factors affecting endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound during IVF treatment, Human Reproduction. 2006, 21(4): 1062-1069.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal blood flow in the prediction of ovarian response during in vitro fertilization treatment, Human Reproduction. 2005, 20(11): 3147-3151.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal vascularity is not predictive of ovarian response and pregnancy, Reproductive BioMedicine Online. 2006, 12: 43-49.
Ng E.H.Y., Preimplantation genetic diagnosis, CME on Obstetrics and Gynaecology organized by Baptist Hospital, July 21, 2005. 2005.
Ng E.H.Y. and Ho P.C., Progestogen as Emergency Contraception, Royal College of Obstetrics and Gynaecology, Dialog. 2005, 4: 2.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Relationship between uterine blood flow and endometrial and subendometrial blood flows during stimulated and natural cycles, Fertility and Sterility. 2006, 85(3): 721-727.
Ng E.H.Y., Specialist life-Dr. Ernest Ng, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2005, 121: 124-125.
Ng E.H.Y., Study on polycystic ovary syndrome, The Fifth International Conference on Reproductive Endocrinology, Chinese Academy of Medicine Sciences and Peking Union Medical College, Beijing, China, 9-12 September, 2005.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during frozen-thawed embryo transfer cycles, Human Reproduction. 2006, 21(6): 1612-1617.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flows measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during IVF treatment, Human Reproduction. 2006, 21(1): 164-170.
Ng E.H.Y., Yeung W.S.B. and Ho P.C., The significance of antral follicle count in controlled ovarian stimulation and intrauterine insemination, Journal of Assisted Reproduction and Genetics. 2005, 22: 323-328.
Ngan E.S.W., Lee K.Y., Yeung W.S.B., Ngan H.Y.S., Ng E.H.Y. and Ho P.C., Endocrine Gland-Derived Vascular Endothelial Growth Factor is expressed in human peri-implantation endometrium, but not in endometrial carcinoma, Endocrinology. 2006, 147: 88-95.
Tang F.O.S., Ong C.Y.T., Tse K.Y., Ng E.H.Y., Lee W.H. and Ho P.C., A randomized trial to compare the use of sublingual misoprostol with or without an additional 1 week course for the management of first trimester silent miscarriage, Human Reproduction. 2006, 21(1): 189-192.


Researcher : Ng TY

Project Title:A propective randomised controlled trial: prevention of lymphedema by omentoplasty after pelvic lymphadenectomy
Investigator(s):Ng TY, Ngan HYS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Low Budget High Impact Programme
Start Date:11/2001
Abstract:
To see whether these preliminary results are validated in a prospective randomised comparison.


Project Title:A prospective randomized controlled trial: prevention of Lymphedema by Omentoplasty after pelvic lymphadenectomy
Investigator(s):Ng TY, Ngan HYS, Chan YM, Tam KF
Department:Obstetrics & Gynaecology
Source(s) of Funding:Health and Health Services Research Fund - Full Grants
Start Date:04/2004
Abstract:
Lymphedema is a debilitating condition that occurs in up to 40% of patients after pelvic lymphadenectomy. New surgical techniques have been advocated to reduce this complication. A pilot study using Omentoplasty observed that 8% of patients after this procedure had clinical lymphedema compared with 40% in historical controls. This study aims to see whether these preliminary results are validated in a prospective randomised comparison.


List of Research Outputs

Chan Y.M., Lee P.W.H., Fong D.Y.T., Fung A.S.M., Wu L.Y.F., Choi A.Y., Ng T.Y., Ngan H.Y.S. and Wong L.C., Effect of individual psychological intervention in Chinese women with gynecologic malignancy: A randomized controlled trial, Journal of Clinical Oncology. 2005, 23(22): 4913-4924.
Huang F.Y., Chiu P.M., Tam K.F., Kwok K.Y., Lau E.T., Tang M.H., Ng T.Y., Liu V.W.S., Cheung A.N.Y. and Ngan H.Y.S., Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer, Gynecologic Oncology. 2006, 103(1): 219-25.
Tam K.F., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Ethnicity is a factor to be considered before dose planning in ovarian cancer patients to be treated with topotecan, International Journal of Gynecological Cancer. 2006, 16: 135-139.
Tam K.F., Chau M.T., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Hepatic arteriogram for gestational trophoblastic tumor: Is it useful? , Cancer Investigation. 2005, 23: 677-682.
Tam K.F., Cheng D.K.L., Ng T.Y. and Ngan H.Y.S., The behaviors of seeking a second opinion from other health-care professionals and the utilization of complementary and alternative medicine in gynecologic cancer patients, Support Care Cancer. 2005, 13: 679-684.


Researcher : Ngai CSW

Project Title:Vaginal misoprostol for first trimester termination of pregnancy prior to 9 weeks of gestation
Investigator(s):Ngai CSW, Tang FOS, Chan YM, Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Health Services Research Fund - Full Grants
Start Date:05/1999
Abstract:
To compare the effectiveness of vaginal misoprostol (water vs no water added) in first trimester termination of pregnancy.


Project Title:Cervical assessment in prediction of preterm labour: is three-dimensional ultrasonography a better choice?
Investigator(s):Ngai CSW, Lao TTH, Chen M, Leung KY
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Abstract:
To compare the usefulness of 3D versus 2D US in prediction of preterm labour.


Project Title:Multiple pregnancy and gestational diabetes: a physiological response or a pathologic condition?
Investigator(s):Ngai CSW, Lao TTH, Leung WC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To investigate the incidence of gestational disbetes mellitus in twin pregnancy; to investigate the changes of glucose metabolism throughout pregnancy in multiple pregnancy; to evaluate the diagnostic criteria of gestational diabetes mellitus in twin pregnancy.


List of Research Outputs

Leung K.Y., Chan K.K.L., Ngai C.S.W., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W. and Tang M.H.Y., How three-dimensional ultrasound help in the prenatal diagnosis of fetal anomalies?, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W., Chan B.C., Leung W.C., Lee C.P. and Tang M.H.Y., Three-dimensional extended imaging: a new display modality for three-dimensional ultrasound examination, Ultrasound Obstetrics and Gynecology. 2005, 26: 244-251.
Ngai C.S.W., Should we screen sexually transmitted diseases for women requesting for termination of pregnancy?, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.
Ting J.Y., Lam B.C.C., Ngai C.S.W., Leung W.C. and Chau K.L., Splenic rupture in a premature neonate, Hong Kong Medical Journal. 2006, 12(1): 68-70.


Researcher : Ngan ESW

Project Title:Sonic hedgehog (Shh) signaling in the enteric neural crest cells
Investigator(s):Ngan ESW, Sham MH, Lui VCH, Tam PKH
Department:Surgery
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:01/2005
Completion Date:12/2006
Abstract:
The main objectives of this project are: 1) To investigate the effect of Shh on the expression profiles of BMP-2 and BMP-4 in mouse embryonic gut. 2) To study the interplay between Shh/BMPs and GDNF signaling in NCC migration. The results of this basic research would provide better understanding of the process of ENS development. This data could further extend the knowledge base regarding the etiology and our understanding of Hirschsprung's disease in human.


Project Title:Novel Function(s) of Prokineticins in Neural Crest Stem Cells
Investigator(s):Ngan ESW, Lui VCH, Tam PKH
Department:Surgery
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:01/2006
Abstract:
The mammalian enteric nervous system (ENS) is derived from the neural crest cells (NCC) which enter the foregut and colonize the entire wall of gastrointestinal tract (1). In mice, NCCs reach foregut at approximately embryonic day 9.4 (E9.5) and finish the colonization by ~E14.5. Numerous gut mesenchyme/mucosa-derived factors and their receptors on NCC are found to be crucial for NCC proliferation, differentiation and migration. They are glial cell-line derived neurotrophic factor (GDNF) and its receptors (RET, GFRA1)(2); endothelin 3 (EDN3) and its receptor (EDNRB)(3); sonic hedgehog (Shh) and its receptor (Ptc)(4). A delicate balance between these signals is a prerequisite for the proper regulation of NCCs during ENS development. Failure to completely colonize the gut results in the absence of enteric ganglia as seen in Hirschsprung’s disease in human.. Prokineticin –1 and -2 (Prok-1 and Prok-2), also referred to endocrine gland derived vascular endothelial growth factor (EG-VEGF) and Bv8, respectively, are structurally related and belong to a newly identified family of secreted proteins, AVIT protein family. These proteins are distributed widely in mammalian tissues. Prok-1 mRNA expression has been described in a variety of tissues, in steroidogenic glands such as the ovary, testis, adrenal gland and placenta, but also in the brain, colon, skeletal muscle, small intestine, spleen, thymus liver, bladder, prostate and uterus(5,6) . Prok-2, on the other hand, shows the highest expression in testis, colon, brain and peripheral blood leukocytes(7,8). These two prokineticins are known to bind to two closely related G protein-coupled receptors, PK-R1 and PK-R2. Receptor activation leads to mobilization of calcium, stimulation of phosphoinositide turnover, and activation of p44/p42 MAPK signaling pathways. Within distinct contexts, the receptors are likely differentially regulated and distinct expression patterns of Prok receptors were observed in various tissues. In the adrenal gland, only PK-R1 was detected. In the ovary, follicular cells predominantly express PK-R1, whereas corpus luteum-derived cells express high levels of both PK-R1 and PK-R2. Similarly, these two receptors are also co-existing in endometrium, but at different expression level. Therefore, it is believed that prokineticins may have different angiogenic as well as non-angiogenic functions in different tissues via acting on either PK-R1 or PK-R2. However, the exact role(s) of PK-R1 and PK-R2 remains unclear. A new appreciation for the intricacy of peptidergic GPCRs is developing, as the ligands for orphan receptors are identified and as distinct signaling and functional responses for multiple ligands and oligomerized receptors are demonstrated. Thus, the distinct or overlapping expression of prokineticins and the possible heteroligomerization of receptors may increase the functional complexity of this system (8,9). It is currently known that prokineticins possess diverse biological functions: they promote angiogenesis in ovary and testis; induce proliferation, migration and fenestration of endothelial cells derived from adrenal gland ; support neuronal survival; cause hyperalgesia of skin; promote contraction of gastrointestinal smooth muscle; and control pain sensation and behavioral circadian rhythms(8,9). More recently, these two receptors were also found in human and mouse hematopoietic stem cells and specific mature blood cells, including lymphocytes. Prokineticins can modulate growth, survival, and function of cells of the innate and adaptive immune systems, possibly through autocrine or paracrine signaling mechanisms(7). Given the reported expression patterns and activities of the mammalian orthologs in brain, reproductive tract, and cells of the immune system, the molecules seem to have subserved similar functions across evolution. In addition to being the angiogenic factors, prokineticins would be the universal survival/mitogenic factors for various cells including endothelial cells, neuronal cells, lymphocytes as well as the hematopietic stem cells. Noteworthy, prokineticins and their receptors were detected in mouse embryo as early at E7, the role(s) of these factors during embryonic development still remains to be disclosed. Our preliminary data: It is known that prokineticin-1 is expressed in mouse and human small intestine and colon. Using antibody specific to Prok-1 to perform the immunohistochemistry study, we found that Prok-1 protein is expressed in the mucosa and enteric neuronal plexus of the gut in the adult mouse. The presence of Prok-1 in neuronal plexus was subsequently confirmed by colocalization study with the neuronal marker, Tuj1 (Appendix I, Figure 1g-i). In embryonic stage, it is highly expressed in the mucosa (E12.5 and E15.5) and forms a concentration gradient across the radius of the embryonic gut (Appendix I, Figure 1a-f). Subsequent RT-PCR analysis also showed that NCCs do not express endogenous Prok-1 (data not shown). A physiological effect of Prok-1 is dependent on the presence of the ligand and the receptor, so we have also examined the expression of the receptors, PK-R1 and PK-R2. Expression study using RT-PCR showed that the PK-R1, but not PK-R2, is expressed in NCCs isolated from E11.5 embryonic gut (Appendix I, Figure 2A). Localization of PK-R1 receptor using in situ hybridization further demonstrated the existence of PK-R1 in the NCCs and NCC derived enteric neurons of the embryonic guts (E10.5-E17.5). The signal is specific because there is no fluorescent signal detected when sense probe was used (Ctrl, Appendix I, Figure 2B). Taken together, it is believed that Prok-1, probably like GDNF, EDN3 and Shh, acts as mesenchyme/mucosa-derived factors and mediate the proliferation or differentiation of NCCs during ENS development. On the other hand, it may also act as a survival factor for the enteric neurons in the adult gut, via an autocrine/paracrine mechanism. Thus, we hypothesize that Prok-1 provides an additional layer of signaling refinement to maintain survival/proliferation of NCCs and mature neurons of the developing and mature ENS, respectively. The aim of this proposed project is to elucidate the physiological role(s) of prokineticins in ENS and during its development. Objective 1: To study the temporal and spatial expression patterns of prokineticins (Prok-1 and Prok-2) and their receptors in mouse gut during ENS development. The information obtained would give a hint to delineate the potential physiological functions of these molecules. Objective 2: To elucidate the mitogenic/neuroprotective properties of prokineticins on NCCs and NCC derived neurons. The functions of prokineticins in NCCs will be examined directly using the in vitro NCC culture system. Objective 3: To examine the effect of prokineticins on the differentiation capacity of NCCs. Reference 1. D. Newgreen, H. M. Young, Pediatr. Dev. Pathol. 5, 329 (2002). 2. A. Barlow, E. de Graaff, V. Pachnis, Neuron 40, 905 (2003). 3. G. M. Kruger et al., Neuron 40, 917 (2003). 4. M. Fu, V. C. Lui, M. H. Sham, V. Pachnis, P. K. Tam, J. Cell Biol. 166, 673 (2004). 5. J. LeCouter et al., Nature 412, 877 (2001). 6. E. S .Ngan et al., Endocrinology Oct 6; [Epub ahead of print] (2005) . 7. J. LeCouter, C. Zlot, M. Tejada, F. Peale, N. Ferrara, Proc. Natl. Acad. Sci. U. S. A 101, 16813 (2004). 8. A. Kaser, et al., EMBO reports 4,469 (2003) 9. J. LeCouter, R. Lin, N. Ferrara Ann. N.Y. Acad. Sci 1014: 50(2004).


List of Research Outputs



Researcher : Ngan HYS

Project Title:High dose cis-platinum and cyclophosphamide vs taxol in ovarian cancer
Investigator(s):Ngan HYS, Wong RLC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Other Funding Scheme
Start Date:07/1994
Abstract:
To study high dose cis-platinum and cyclophosphamide vs taxol in ovarian cancer.


Project Title:Rapid identification of tumour-specific gene methylation at multiple loci in gynaecological cancers
Investigator(s):Ngan HYS, Liu VWS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To determine and differentiate the methylation status for 30 gene loci among the three gynaecological cancers using a pooled DNA approach.


Project Title:The roles of p73 and N-terminal truncated p73 isoforms in chemosensitivity of ovarian cancer
Investigator(s):Ngan HYS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2005
Completion Date:06/2006
Abstract:
N/A


Project Title:Functional analysis of AMP-activated protein kinase (AMPK) in cervical cancer cell growth
Investigator(s):Ngan HYS, Liu VWS
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2005
Completion Date:10/2006
Abstract:
Introduction: Cervical carcinoma is the most common cancer of the female genital tract and is still a major cause of morbidity and mortality in women of Hong Kong. Human papillomaviruses (HPVs) have been found in nearly all cases of cervical cancer, and it has been well established to be an important aetiological factor for the development of the cancer (zur Hausen, 2002). Although HPV infection is generally believed to be the initiating factor for cervical cancer development, the number of HPV infected women is much higher than the number of women with cervical cancer. Therefore, the transformation from low-grade lesion to invasive cancer must involve other factors such as cellular genetic changes affecting oncogenes and/or tumor suppressor genes. Our preliminary work: Consistent with other studies, we have been able to detect frequent amplifications of chromosomal regions at 5p and 3q in cervical tumors and cervical cancer cell lines using comparative genomic hybridization (CGH) technology (Huang et al, 2005). This suggests that 5p and 3q may harbor putative oncogenes that may play an important role in the development of cervical cancer through gene amplification and over-expression. Subsequently, four specific genes located on the chromosome 5 have been randomly selected, including the catalytic alpha-1 subunit of the AMP-activated protein kinase (AMPK alpha-1) (at 5p12), polymerase (DNA directed) sigma (POLS) (at 5p15), delta 2 catenin (CTNND2) (at 5p15.2) and ERBB2 interacting protein (ERBB2IP) (at 5q12.3), for gene copy number measurement using fluorescent PCR. Table 1 indicates the degrees of gene amplification of the above four genes in various cervical cancer cell lines compared with DNA from normal lymphocyte. Results indicate that all the four genes were amplified in cervical cancer cell lines (Huang et al, manuscript submitted for publication). The greatest fold of amplification was seen in the AMPK alpha-1 gene ranging from 2.8 to 6.3. TABLE 1. Degrees of gene amplification measured by semi-quantitative fluorescent PCR Cell lines AMPK alpha-1 POLS CTNND2 ERBB2IP CasKi 6.1 2.0 3.5 2.3 C33A 2.8 1.5 2.0 2.0 HeLa 6.3 2.7 4.7 2.0 SiHa 4.2 4.2 5.3 1.6 In addition to cervical cancer cell lines, gene amplification in tumour samples was also investigated. Again, the most frequently amplified gene was seen in AMPK alpha-1. Over-expression of AMPK alpha-1 was also detected using RT-PCR and immunohistochemical staining (Huang et al, manuscript submitted for publication). Thus, our preliminary data obtained suggests a relation between AMPK alpha-1 over-expression and cervical cancer. AMPK has been well recognized as an important mediator of stress signals including nutrient deprivation, physical stresses and hypoxia making the cells more tolerance to adverse conditions (Hardie et al, 1998; Kemp et al, 1999; Leclerc et al, 2002a; Leclerc et al, 2002b). We therefore hypothesized that AMPK expression could be an important factor in cancer development, in maintaining cancer cell survival, and in conferring aggressive behaviour of tumours. PROJECT OBJECTIVES [1] To investigate the cervical cancer cell growth under stress with or without AMPK activation. [2] To investigate the association between the AMPK expression with clinical features of cervical cancer. References: (1) zur Hausen, H. Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer. 2002;2:342-50. (2) Huang FY, Kwok YKY, Lau ETK, Tang MHY, Ng TY, and Ngan HYS. Genetic Abnormalities and HPV Status in Cervical and Vulvar Squamous Carcinomas. Cancer, Genetics, and Cytogenetics. 2005;157:42-48. (3) Huang FY, Chiu PM, Kwok YKY, Lau ETK, Tang MHY, Ng TY, Tam KF, Cheung ANY, Liu VWS, and Ngan HYS. Semi-quantitative Fluorescent PCR Analysis identifies PRKAA1 and ERBIN on Chromosome 5 as Potential Candidate Cancer Genes for Cervical Cancer (Submitted for publication). (4) Hardie DG, Carling D, and Carlson M. The AMP-activated/SNF1 protein kinase subfamily: metabolic sensors of the eukaryotic cell? Annu Rev Biochem. 1998;67:821-55. (5) Kemp BE, Mitchelhill KI, Stapleton D, Michell BJ, Chen ZP, and Witters LA. Dealing with energy demand: the AMP-activated protein kinase. Trends Biochem Sci. 1999;24:22-5. (6) Leclerc I, da Silva Xavier G, and Rutter GA. AMP- and stress-activated protein kinases: key regulators of glucose-dependent gene transcription in mammalian cells? Prog Nucleic Acid Res Mol Biol. 2002a;71:69-90. (7) Leclerc I, Viollet B, da Silva Xavier G, Kahn A, and Rutter GA. Role of AMP-activated protein kinase in the regulation of gene transcription. Biochem Soc Trans. 2002b;30:307-11.


List of Research Outputs

Chan K.K.L., Lam K.W., Tam K.F. and Ngan H.Y.S., Effectiveness of a single dose methotrexate regime in treatment of low risk gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Chan K.K.L. and Ngan H.Y.S., Should women with treated genital tract cancers start hormone replacement?, Journal of Paediatrics, Obstetrics and Gynaecology. 2005, Nov/Dec: 245248.
Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Chan K.H., Mak W.W., Ngan H.Y.S., Xu X., Screaton G., Tam P.K.H., Austyn J.M., Chan L.C., Yip S.P., Peiris J.S.M., Khoo U.S. and Lin C.L., Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection. , Nature Genetics. 2006, 38: 38-46.
Chan Y.M., Lee P.W.H., Fong D.Y.T., Fung A.S.M., Wu L.Y.F., Choi A.Y., Ng T.Y., Ngan H.Y.S. and Wong L.C., Effect of individual psychological intervention in Chinese women with gynecologic malignancy: A randomized controlled trial, Journal of Clinical Oncology. 2005, 23(22): 4913-4924.
Cheung A.N.Y., Chiu P.M., Chan Q.K.Y., Chan Y.K. and Ngan H.Y.S., Expression of Stem cell related genes in normal placentas and Gestational Trophoblastic Diseases, 11th Conference of the European Placenta Group, Glasgow, 2-8th September. 2005.
Cheung W.T., Au S.C.L., Cheung A.N.Y., Ngan H.Y.S. and Wong A.S.T., Pigment Epithelium-Derived Factor, a Gene Down-Regulated by Estrogen, Mediates Growth Inhibition in Human Ovarian Surface Epithelial and Ovarian Cancer Cells, 88th Annual Meeting of the Endocrine Society, Boston, MA, p.644 (Abstract No. P3-32). 2006.
Cheung W.T., Au S.C., Cheung A.N.Y., Ngan H.Y.S., Tombran-Tink J., Auersperg N. and Wong A.S.T., Pigment epithelium-derived factor is estrogen sensitive and inhibits the growth of human ovarian cancer and ovarian surface epithelial cells, Endocrinology. 2006, 147(9): 4179-91.
Chiu P.M., Feng H., Benbrook D.M., Ngan H.Y.S., Khoo U.S., Xue W., Tsao G.S.W., Chan K.W. and Cheung A.N.Y., Effect of all-trans retinoic acid on tissue dynamics of choriocarcinoma cell lines: an organotypic model , Journal of Clinical Pathology . 2006, 59(8): 845-50.
Chiu P.M., Feng H., Behbrook D.M., Ngan H.Y.S., Khoo U.S., Xue W., Tsao G.S.W., Chan K.W. and Cheung A.N.Y., Tissue dynamic effect of all-trans retinoic acid on choriocarcinoma an organotypic model, XIII World Congress On Gestational Trophoblastic Diseases, Hong Kong, October 23-26. 2005.
Feng H., Tsao G.S.W., Ngan H.Y.S., Xue W., Chiu P.M. and Cheung A.N.Y., Differential expression of insulin-like growth factor binding protein 1 and ferritin light polypeptide in gestational trophoblastic neoplasia: combined cDNA suppression subtractive hybridization and microarray study, Cancer. 2005, 104(11): 2409-2416.
Feng H., Tsao G.S.W., Ngan H.Y.S., Kwan H.S., Shih S.M., Xue W., Chiu P.M., Chan K.W. and Cheung A.N.Y., Differential gene expression identified in complete hydatidiform mole by combining suppression subtractive hybridization and cDNA microarray, Placenta. 2006, 27: 521-526.
Feng H., Tsao G.S.W., Ngan H.Y.S., Xue W., Chiu P.M., Kwan H.S., Shih S.M. and Cheung A.N.Y., Prostate Stem Cell Antigen is a genetic indicator for development of gestational trophoblastic neoplasia, American Association for Cancer Research's 97th Annual Meeting, March 31-April 5, 2006, in Washington DC, USA. 2006.
Feng H., Tsao G.S.W., Ngan H.Y.S., Xue W., Chiu P.M., Kwan H.S., Shih S.M. and Cheung A.N.Y., Prostate stem cell antigen is over-expressed in persistent hydatidiform mole, XIII World Congress On Gestational Trophoblastic Diseases, Hong Kong, October 23-26. 2005.
Fong P.Y., Xue W., Ngan H.Y.S., Chiu P.M., Chan Y.K., Tsao G.S.W. and Cheung A.N.Y., Caspase activity is downregulated in choriocarcinoma: a cDNA array differential expression study, Journal of Clinical Pathology. 2006, 59: 179-183.
Huang F.Y., Chiu P.M., Tam K.F., Kwok K.Y., Lau E.T., Tang M.H., Ng T.Y., Liu V.W.S., Cheung A.N.Y. and Ngan H.Y.S., Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer, Gynecologic Oncology. 2006, 103(1): 219-25.
Ip P.P.C., Chan K.K.L., Cheung A.N.Y. and Ngan H.Y.S., Choriocarcinomas following full-term non-molar gestations, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Khoo U.S., Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Ngan H.Y.S., Tam P.K.H., Chan L.C., Yip S.P., Peiris J.S.M., Sham P.C. and Lin C.L., The protective role of homozygous L-SIGN (CLEC4M) in SARS coronavirus infection., HUGO's 11th Human Genome Meeting, Helsinki, Finland. . 2006.
Liu S., Chan Y.K., Leung C.Y., Law H.K.W., Leung T.W. and Ngan H.Y.S., Enhancement of the radiosensitivity of cervical cancer cells by overexpressing p73a, Molecular Cancer Therapeutics. USA, American Association of Cancer Research, 2006, 5: 1209-1215.
Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes In The Mitochondrial Content Of Cervical Cancer Cells Under Stress, The 3rd Asain Society for Mitochondrial Research and Medicine December 14-17, 2005, Tokyo, Japan. 2005.
Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes in mitochondrial content of cervical cancer cells under stress, The 3rd Conference of the Asian Society for Mitochondrial Research and Medcine , December 14-17, 2005, Tokyo, Japan. 2005.
Ngan E.S.W., Lee K.Y., Yeung W.S.B., Ngan H.Y.S., Ng E.H.Y. and Ho P.C., Endocrine Gland-Derived Vascular Endothelial Growth Factor is expressed in human peri-implantation endometrium, but not in endometrial carcinoma, Endocrinology. 2006, 147: 88-95.
Ngan H.Y.S., "Role of chemotherapy in gestational trophoblastic diseases" and "Psychological aspects in gestational trophoblastic disease", First National Gestational Trophoblastic Disease Seminar, Bendung, Indonesia, April 7-8, 2006. 2006.
Ngan H.Y.S., Bailliere's Best Practice and Research on Gestational Trophoblastic Diseases-2003, Guest Editor of Clinical Obstetrics and Gynaecology . 2005.
Ngan H.Y.S., Editor of "Staging Classification and Clinical Practice Guidelines of Gynaecologic Cancers" 2003. 2005, Second Edition.
Ngan H.Y.S., Editorial Board Member - Hong Kong Journal of Gynaecology, Obstetrics and Midwifery 1998. 2005.
Ngan H.Y.S., Editorial Board Member - International Journal of Gynecologic Cancer 2001. 2005.
Ngan H.Y.S., Editorial Board Member - Journal of Clinical Oncology (Chinese Edition) 2000. 2005.
Ngan H.Y.S., Editorial Board Member - Journal of Obstetrics and Gynaecology Research 1997. 2005.
Ngan H.Y.S., Editorial Board Member - Journal of Paediatrics, Gynaecology and Obstetrics 1997. 2005.
Ngan H.Y.S., Editorial Board Member - The Hong Kong Medical Journal. 2005.
Ngan H.Y.S., Editorial Board member - Gynecologic Oncology 2004. 2005.
Ngan H.Y.S., HPV and cervical cancer, 12th Hong Kong International Cancer Congress and 2nd Annual Meeting, Centre for Cancer Research, Hong Kong, December 8-10, 2005. 2005.
Ngan H.Y.S., Keynote lecture on "Training of gynaecological oncologist", National Conference on Gynaecologic Oncology, "How to train oncologist in China", Shengyang, China, June 26-28, 2006. 2006.
Ngan H.Y.S., Management of endometrial cancer, AOCOG 2005 Pre-congress International Scientific Conference on "Improving Women's Health: Learning by Sharing Experience", UlaanBaatar, Mongolia, September 27-28, 2005. 2005.
Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K.L., Methotrexate, bleomycin and etoposide (MBE) in the treatment of gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K., Methotrexate, bleomycin, and etoposide in the treatment of gestational trophoblastic neoplasia , Obstetrics and Gynecology. 2006, 107(5): 1012-1017.
Ngan H.Y.S., Molecular studies in Gynaecological Cancers - Seminar: "Management of gestational trophoblastic neoplasia, First Beijing International Conference on Obstetrics and Gynaecology, Beijing, China, October 7-10, 2005. 2005.
Ngan H.Y.S., Overview in the management of cervical cancer, 2nd Jiangsu Conference on Diagnosis and Management of Cervical Lesions, Nanjing, China, December 4, 2005. 2005.
Ngan H.Y.S., Revised staging of gestational trophoblastic diseases, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005. 2005.
Ngan H.Y.S., Lam K.W., Tam K.F. and Chan K.K.L., Treatment of recurrent gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Siu K.Y., Woo N.W.S., Ngan H.Y.S. and Cheung A.N.Y., Differential expression of p21-activated kinases (Paks) in ovarian cancer, American Association for Cancer Research's 97th Annual Meeting, March 31-April 5, 2006, in Washington DC, USA. (Dr Siu received the Avon Foundation-AACR International Scholar-in-Training Grant). 2006.
Siu K.Y., Yeung C.W., Chiu P.M., Feng H., Ngan H.Y.S., Xue W. and Cheung A.N.Y., p21-activated kinases (Paks) in gestational trophoblastic disease, XIIIth World Congress on Gestational Trophoblastic Diseases. Proceedings P.103. 2005.
Tam K.F., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Ethnicity is a factor to be considered before dose planning in ovarian cancer patients to be treated with topotecan, International Journal of Gynecological Cancer. 2006, 16: 135-139.
Tam K.F., Chau M.T., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Hepatic arteriogram for gestational trophoblastic tumor: Is it useful? , Cancer Investigation. 2005, 23: 677-682.
Tam K.F., Cheng D.K.L., Ng T.Y. and Ngan H.Y.S., The behaviors of seeking a second opinion from other health-care professionals and the utilization of complementary and alternative medicine in gynecologic cancer patients, Support Care Cancer. 2005, 13: 679-684.
Tse K.Y., Chan K.K.L., Tam K.F. and Ngan H.Y.S., Local experience of the management of profuse bleeding in gestational trophoblastic diseases, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Wang Y., Liu V.W.S., Tsang P.C.K., Chiu P.M., Cheung A.N.Y., Khoo U.S., Nagley P. and Ngan H.Y.S., Microsatellite instability in mitochondrial genome of common female cancers, International Journal of Gynecological Cancer. 2006, Suppl 1: 259-266.
Wang Y., Liu V.W.S., Xue W., Tsang P.C.K., Cheung A.N.Y. and Ngan H.Y.S., The Increase Of Mitochondrial Dna Content In Endometrial Adenocarcinoma Cells: A Quantitative Study Using Laser-captured Microdissected Tissues, Gynecologic Oncology. 2005, 98: 104-110.
Xue W., Feng H., Chan Y.K., Chiu P.M., Ngan H.Y.S., Khoo U.S., Tsao G.S.W., Chan K.W. and Cheung A.N.Y., Id helix-loop-helix proteins are differentially expressed in gestational trophoblastic disease., Histopathology. 2005, 47(3): 303-9.
Yeung M., Pun T.C., Chan K., Ngan H.Y.S., Ip P.P.C. and Cheung A.N.Y., Negative pregnancy test and trophoblastic lesions: from ectopic abortion to choriocarcinoma, XIII World Congress On Gestational Trophoblastic Diseases, Hong Kong, October 23-26. 2005.


Researcher : Ong CYT

Project Title:To determine the correlation of changes in maternal levels of pregnancy-associated plasma protein A (PAPP-A) and free [beta] human chorionic gonadotrophin (F[beta]-hCG) with placental development and fetal outcomes
Investigator(s):Ong CYT, Lao TTH, Leung WC, Leung KY, Lam HSW
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Abstract:
To correlate the serial changes of maternal serum PAPP-A and free [beta]-hCG concentrations with placental volume and vascular indices, swell as fetal size, as assessed by 3-D/4-D ultrasonographic studies throughout pregnancy; to determine whether different patterns in the changes of PAPP-A and free [beta]-hCG in different trimesters will be predictive of different patterns of placental development and fetal growth, and whether this can help to identify specifically the eventual complications through the association with the various growth and developmental patterns.


Project Title:Assessment of the interrelationship among the angiogenic growth factors and topological properties of placental villous capillaries with fetal growth, development of the placenta and its vasculature progressively throughout the course of pregnancy, and pregnancy outcomes
Investigator(s):Ong CYT
Department:Obstetrics & Gynaecology
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2004
Abstract:
N/A


Project Title:First trimester prediction of fetal α-thalassaemia-1 with maternal serum markers, nuchal translucency measurement and three dimentional ultrasound measurement of placental volume
Investigator(s):Ong CYT, Chen M, Leung KY, Tang MHY, Lee CP
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To measure 3D ultrasound placental and fetal volume at 10-14 weeks of gestation, and to determine whether different changes in the placental volume is predictive of fetuses affected by α-thalassaemia-1; to measure maternal serum PAPP-A and free β-hCG concentrations in fetuses affected and unaffected by β-thalassaemia-1 at 10-14 weeks of gestation, and determine the predictive value of these markers along with or without NT measurement in diagnosis of fetuses affected by α-thalassaemia-1.


Project Title:Assessment of the interrelationship among the angiogenic growth factors and topological properties of placental villous capillaries with fetal growth, development of the placenta and its vasculature progressively throughout the course of pregnancy, and pregnancy outcomes
Investigator(s):Ong CYT
Department:Obstetrics & Gynaecology
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2005
Completion Date:06/2006
Abstract:
N/A


List of Research Outputs

Tang F.O.S., Ong C.Y.T., Tse K.Y., Ng E.H.Y., Lee W.H. and Ho P.C., A randomized trial to compare the use of sublingual misoprostol with or without an additional 1 week course for the management of first trimester silent miscarriage, Human Reproduction. 2006, 21(1): 189-192.


Researcher : Pun TC

List of Research Outputs

Chan C.P., Leung W.C., Leung K.Y., Pun T.C. and Lee C.P., Confirmation of first trimester fetal abnormalities at termination of pregnancy, The 15th World Congress on Ultrasound in Obstetrics and Gynecology (ISUOG 2005), September 25-29, 2005, Vancouver, Canada. 2005.
Lam K.W., Wong H.S. and Pun T.C., The practice of episiotomy in public hospitals in Hong Kong, Hong Kong Medical Journal. 2006, 12(2): 94-98.
Li I.C.F., Lau W.N.T. and Pun T.C., Umbilical wound hernia following a 5-mm threaded cannula wound with primary repair stitch in situ, Acta Obstetricia et Gynecologica Scandinavica. 2005, 84: 1125-1126.
Tam K.F., Lee C.P. and Pun T.C., Mild postnatal anemia: Is it a problem?x, American Journal of Perinatology. 2005, 22(7): 345-349.
Yeung M., Pun T.C., Chan K., Ngan H.Y.S., Ip P.P.C. and Cheung A.N.Y., Negative pregnancy test and trophoblastic lesions: from ectopic abortion to choriocarcinoma, XIII World Congress On Gestational Trophoblastic Diseases, Hong Kong, October 23-26. 2005.


Researcher : Tam KF

List of Research Outputs

Chan K.K.L., Lam K.W., Tam K.F. and Ngan H.Y.S., Effectiveness of a single dose methotrexate regime in treatment of low risk gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Huang F.Y., Chiu P.M., Tam K.F., Kwok K.Y., Lau E.T., Tang M.H., Ng T.Y., Liu V.W.S., Cheung A.N.Y. and Ngan H.Y.S., Semi-quantitative fluorescent PCR analysis identifies PRKAA1 on chromosome 5 as a potential candidate cancer gene of cervical cancer, Gynecologic Oncology. 2006, 103(1): 219-25.
Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K.L., Methotrexate, bleomycin and etoposide (MBE) in the treatment of gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Ngan H.Y.S., Tam K.F., Lam K.W. and Chan K.K., Methotrexate, bleomycin, and etoposide in the treatment of gestational trophoblastic neoplasia , Obstetrics and Gynecology. 2006, 107(5): 1012-1017.
Ngan H.Y.S., Lam K.W., Tam K.F. and Chan K.K.L., Treatment of recurrent gestational trophoblastic neoplasia, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.
Tam K.F., Atypical glandular cells in cervical smear during pregnancy and postpartum period, Clinical Medicine and Research. 2005, 3(1): 1-2.
Tam K.F., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Ethnicity is a factor to be considered before dose planning in ovarian cancer patients to be treated with topotecan, International Journal of Gynecological Cancer. 2006, 16: 135-139.
Tam K.F., Chau M.T., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Hepatic arteriogram for gestational trophoblastic tumor: Is it useful? , Cancer Investigation. 2005, 23: 677-682.
Tam K.F., Lee C.P. and Pun T.C., Mild postnatal anemia: Is it a problem?x, American Journal of Perinatology. 2005, 22(7): 345-349.
Tam K.F., Cheng D.K.L., Ng T.Y. and Ngan H.Y.S., The behaviors of seeking a second opinion from other health-care professionals and the utilization of complementary and alternative medicine in gynecologic cancer patients, Support Care Cancer. 2005, 13: 679-684.
Tse K.Y., Chan K.K.L., Tam K.F. and Ngan H.Y.S., Local experience of the management of profuse bleeding in gestational trophoblastic diseases, XIIIth World Congress on Gestational Trophoblastic Diseases, The International Society for the Study of Trophoblastic Diseases and The University of Hong Kong, 23-26 October, 2005.


Researcher : Tang FOS

Project Title:A prospective randomized comparison of sublingual and vaginal misoprostol in termination of pregnancy in the second trimester
Investigator(s):Tang FOS, Ho PC
Department:Obstetrics & Gynaecology
Source(s) of Funding:Other Funding Scheme
Start Date:10/2000
Abstract:
To compare the efficacy of sublingual and vaginal misoprostol in termination of second trimester pregnancy.


List of Research Outputs

Chan C.C.W., Ng E.H.Y., Tang F.O.S., Yeung W.S.B., Lau E.Y.L. and Ho P.C., A prospective, randomized, double-blind study to compare two doses of recombinant human chorionic gonadotropin in inducing final oocyte maturity and the hormonal profile during the luteal phase, The Journal of Clinical Endocrinology and Metabolism. 2005, 90(7): 3933-3938.
Chan C.C.W., Tang F.O.S., Lau W.N.T. and Tang G.W.K., Bone turnover in young hypoestrogenic women on hormonal therapy, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 124: 204-206.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Circadian changes in uterine artery and ovarian stromal blood flow after pituitary down-regulation, Journal of The Society for Gynecology Investigation. 2005, 12(6): 452-455.
Chan C.C.W., Koo M.W.L., Ng E.H.Y., Tang F.O.S., Yeung W.S.B. and Ho P.C., Effects of Chinese green tea on weight, and hormonal and biochemical profiles in obese patients with polycystic ovary syndrome - A randomized placebo-controlled trial, Journal of The Society for Gynecology Investigation. 2006, 13(1): 64-68.
Chan C.C.W., Ng E.H.Y., Tang F.O.S. and Ho P.C., Endometrial, subendometrial, and ovarian stromal blood flow in patients treated with two different doses of recombinant human chorionic gonadotropin , Fertility and Sterility. 2006, 85(2): 520-522.
Ho P.C., Chan C.C.W., Tang F.O.S. and Ng E.H.Y., Emergency contraception, Gynaecology Forum. 2005, 10(4): 16-18.
Ng E.H.Y., Chan C.C.W., Tang F.O.S. and Ho P.C., Comparison of endometrial and subendometrial blood flows among patients with and without hydrosalpinx shown on scanning during in vitro fertilization treatment, Fertility and Sterility. 2006, 85(2): 333-338.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Factors affecting endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound during IVF treatment, Human Reproduction. 2006, 21(4): 1062-1069.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal blood flow in the prediction of ovarian response during in vitro fertilization treatment, Human Reproduction. 2005, 20(11): 3147-3151.
Ng E.H.Y., Tang F.O.S., Chan C.C.W. and Ho P.C., Ovarian stromal vascularity is not predictive of ovarian response and pregnancy, Reproductive BioMedicine Online. 2006, 12: 43-49.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Relationship between uterine blood flow and endometrial and subendometrial blood flows during stimulated and natural cycles, Fertility and Sterility. 2006, 85(3): 721-727.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during frozen-thawed embryo transfer cycles, Human Reproduction. 2006, 21(6): 1612-1617.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flows measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during IVF treatment, Human Reproduction. 2006, 21(1): 164-170.
Tang F.O.S., Chan C.C.W., Kan A.S.Y. and Ho P.C., A prospective randomized comparison of sublingual and oral misoprostol when combined with mifepristone for medical abortion at 12-20 weeks gestation, Presented at The 54th Meeting of the American Society of Reproductive Medicine, Montreal, Canada, October 15-20, 2005.
Tang F.O.S., Chan C.C.W., Kan A. and Ho P.C., A prospective randomized comparison of sublingual and oral misoprostol when combined with mifepristone for medical abortion at 12-20 weks gestationl, Human Reproduction. 2005, 20(11): 3062-3066.
Tang F.O.S., Ong C.Y.T., Tse K.Y., Ng E.H.Y., Lee W.H. and Ho P.C., A randomized trial to compare the use of sublingual misoprostol with or without an additional 1 week course for the management of first trimester silent miscarriage, Human Reproduction. 2006, 21(1): 189-192.
Tang F.O.S. and Ho P.C., Medical management of first-trimester miscarriages, Journal of Paediatrics, Obstetrics and Gynaecology. 2006, 32(3) May/Jun: 102-105.
Tang F.O.S. and Ho P.C., Termination of pregnancy, In: Edited by J.M.G. van Vugt, L.P. Shulman, Prenatal Medicine. New York, London, Taylor & Francis, 2005, Chapter 28: 509-520.


Researcher : Tang GWK

Project Title:A cross sectional health care study of Chinese perimenopausal women in Hong Kong
Investigator(s):Tang GWK, , Chan M., Tse L.Y.
Department:Obstetrics & Gynaecology
Source(s) of Funding:Other Funding Scheme
Start Date:01/1993
Abstract:
To study Chinese women in Hong Kong on: 1) their perception and understanding of the menopause; 2) their symptoms expressed and experienced before, during and after the menopause; 3) bone density in various age groups ranging from adolescence to postmenopause and to correlate such values with biophysical parameters and diet; 4) how best health care strategies can be planned based on the findings in the study so that women's needs are met most cost effectively.


Project Title:Fracture incidence reduction and safety of TSE-424 (Bazedoxifene Acetate) compared to placebo and Raloxifene in osteoporotic postmenopausal women / Endomentrial Safety Substudy
Investigator(s):Tang GWK
Department:Obstetrics & Gynaecology
Source(s) of Funding:Wyeth Austral PTY Ltd - General Award
Start Date:02/2002
Abstract:
(A) This reduction in the circulating levels of oestrogen during menopause is associated with a number of changes, including osteoporosis. Osteoporosis is characterized by a loss of bone mass and micro architectural deteroration of bones tissue, with a consequent increase in bone fragility and susceptibility to bone fracture. (B) Bazedoxifene acetate is a selective oestrogen receptor modulator (SERM). It has been designed to exhibit the positive effects of an oestrogen agonist on the skeletal and cardiovascular systems, while acting as an antagonist on the uterus and the breast. SERMs have 2 principal indications in the clinic: the prevenetion and treatment of postmenopausal osteoporsis(raloxifene) and the prevention and treatment of breast cancer (tamoxifen), mainly in postmenopausal women. (C) Pre-clinical studies suggest that bazedoxifene acetate protects bone at a lower dose than currently marketed SERMs with no uterine agonist effect, a low potentional to induce flushes, and an improvement in the serum lipid profile. Pre-clinical data has also demonstrated that bazedoxifene acetate effectively suppress the proliferation of breast cancer cell lines, which suggests that it may exhibit antagonist activity on breast cell proliferation. (D) This is a multi-centre, double-blind, randomised, placebo and active comparator controlled phase 3 trial that will involve approximately four thousand patients. There will be about 180 sites participating in the study, which will be conducted globally. Of the four thousand patients recrutied into the study, 1000 patients will be recruited to one of the following four study groups: 1. bazedoxifence acetate 20mg, 2. bazedoxifence acetate 40mg, 3. raloxifece 60mg, 4. placebo.


Project Title:Main Study (013-01)- A study to Evaluate the Efficacy of Quadrivalent HPV (Types 6,11,16 and 18) L1 Virus-Like Particle (VLP) Vaccine in Reducing the Incidence of HPV 6/11-, 16- and 18- Related CIN, and HPV 16 and 18-Related AIS and Cervical Cancer, and HPV 6/11-, 16-, and 18-Related External Genital Warts, VIN and VaIN, and HPV 16 and 18-Related Vulvar and Vaginal Cancer in 16- to 23- Year-Old Women -- The F.U.T.U.R.E. I Study (Females United to Unilaterally Reduce Endo/Ectocervical disease). Substudy (012-01)- Immunogenicity and Safety of Quadrivalent HPV (Types 6,11,16,18)L1 Virus-Like Particle (VLP) Vaccine in 16- to 23-Year-Old Women With an Immunogenicity Bridge Between the HPV 16 Component of the Qualdrivalent Vaccine and the Monovalent HPV 16 Vaccine Pilot Manufacturing Material - The F.U.T.U.R.E. I Study (Females United to Unilaterally Reduce Endo/Ectocervical Disease)
Investigator(s):Tang GWK
Department:Obstetrics & Gynaecology
Source(s) of Funding:Merck, Sharp and Dohme (Asia) Ltd. - General Award
Start Date:08/2002
Abstract:
To demonstrate that a 3-dose regimen of quadrivalent HPV (Types 6,11,16,18) L1 VLP vaccine is generally well tolerated; to demonstrate that quadrivalent HPV vaccine is generally well tolerated.


List of Research Outputs

Chan C.C.W., Lau W.N.T., Chiu S.P., Chen L.C., Choi W.K. and Tang G.W.K., A pilot study on the effects of a Chinese herbal preparation on menopausal symptoms, Gynecological Endocrinology. 2006, 22(2): 70-73.
Chan C.C.W., Tang F.O.S., Lau W.N.T. and Tang G.W.K., Bone turnover in young hypoestrogenic women on hormonal therapy, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 124: 204-206.
Leung K.Y., Ling M. and Tang G.W.K., Use of hormone replacement therapy in the Hong Kong public health sector after the women's health initiative trial, Maturitas. 2005, 52: 277-285.
Tang G.W.K., Continuous Professional Development, 3rd Congress of Asian Medical Education Association (AMEA), Seoul, Korea. 2005.
Tang G.W.K., Continuous professional development, 3rd Asian Pacific Medical Education Conference, Singapore, February 18-21, 2006.
Tang G.W.K., Life-long learning: Whose responsibility is it?, 8th Medical Education Conference for China Mainland, Taiwan and The Hong Kong Region, The Chinese University of Hong Kong, December 12-16, 2005.
Tang G.W.K., Postmenopausal hormone therapy: Where are we now?, Journal of Paediatrics, Obstetrics and Gynaecology. 2005, Jul/Aug 2005: 169-175.
Tang G.W.K., Will Chinese Medicine be the Health-Care Portion for Climacteric, The 6th Asian & Oceanic Congress of Obstetrics and Gynaecology, Seoul, Korea. 2005.
Tang G.W.K., Will Chinese medicine be the health-care potion for climacteric women? , The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.


Researcher : Tang MHY

List of Research Outputs

Chen M., Leung K.Y., Lee C.P., Tang M.H.Y. and Ho P.C., Placental volume measured by three-dimensional ultrasound in the prediction of fetal ao-thalassemia: a preliminary report, Ultrasound Obstetrics and Gynecology. 2006, 28: 166-172.
Chen M., Chan G.S., Lee C.P. and Tang M.H.Y., Sonographic features of lethal multiple pterygium syndrome at 14 weeks, Prenatal Diagnosis. 2005, 25: 475-478.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, Prenatal Diagnosis. 2006, 26: 510-513.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, The 13th International Conference on Prenatal Diagnosis and Therapy, Kyoto, Japan, 28 - 31 May. 2006.
Hui P.W., Lee C.P., Tang M.H.Y. and Ho P.C., Nuchal translucency in pregnancies conceived after assisted reproduction technology, Current Opinion in Obstetrics and Gynecology. 2006, 18: 319-324.
Lau E.T.K., Kwok K.Y., Leung K.Y. and Tang M.H.Y., Automating non-invasive prenatal diagnosis of HB Bart's disease on immunofluorescence staining of fetal erythrocytes in maternal blood, The 13th Conference on Prenatal Diagnosis and Therapy, May 28-31, 2006, Kyoto, Japan. 2006.
Leung K.Y., Chan K.L., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for severe twin-twin transfusion syndrome: A case report, Journal of Paediatrics, Obstetrics and Gynaecology. 2005, Sep/Oct: 202-203.
Leung K.Y., Chan K.K.L., Ngai C.S.W., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W. and Tang M.H.Y., How three-dimensional ultrasound help in the prenatal diagnosis of fetal anomalies?, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, Seoul, Korea, October 1-5, 2005.
Leung K.Y., Ngai C.S.W., Chan B.C., Leung W.C., Lee C.P. and Tang M.H.Y., Three-dimensional extended imaging: a new display modality for three-dimensional ultrasound examination, Ultrasound Obstetrics and Gynecology. 2005, 26: 244-251.
Leung W.C., Lam H.S.W., Lao T.T.H. and Tang M.H.Y., Biochemical hypothyroidism-A new finding in morror syndrome?, European Journal of Obstetrics and Gynecology and Reproductive Biology. 2006, 125(2): 269-271.
Tse K.Y., Leung W.C., Leung K.Y., Lee C.P., Ng L.K.L., Lau E.T., Chan V.N.Y. and Tang M.H.Y., Full karyotyping, rapid aneuploidy diagnosis or both when invasive prenatal testing is performed for diagnosis of thalassaemia?, Molecular Human Reproduction. 2006, 12(1): 55-59.


Researcher : Tsang HY

List of Research Outputs

Chiu C.N., Chung M.K., Tsang H.Y., Koistinen R., Koistinen H., Seppala M., Lee C.K.F. and Yeung W.S.B., Glycodelin-S in human seminal plasma reduces cholesterol efflux and inhibits capacitation of spermatozoa, The Journal of Biological Chemistry. 2005, 280(27): 25580-25589.


Researcher : Tsang PCK

List of Research Outputs

Wang Y., Liu V.W.S., Tsang P.C.K., Chiu P.M., Cheung A.N.Y., Khoo U.S., Nagley P. and Ngan H.Y.S., Microsatellite instability in mitochondrial genome of common female cancers, International Journal of Gynecological Cancer. 2006, Suppl 1: 259-266.
Wang Y., Liu V.W.S., Xue W., Tsang P.C.K., Cheung A.N.Y. and Ngan H.Y.S., The Increase Of Mitochondrial Dna Content In Endometrial Adenocarcinoma Cells: A Quantitative Study Using Laser-captured Microdissected Tissues, Gynecologic Oncology. 2005, 98: 104-110.


Researcher : Wang Y

List of Research Outputs

Wang Y., Liu V.W.S., Tsang P.C.K., Chiu P.M., Cheung A.N.Y., Khoo U.S., Nagley P. and Ngan H.Y.S., Microsatellite instability in mitochondrial genome of common female cancers, International Journal of Gynecological Cancer. 2006, Suppl 1: 259-266.
Wang Y., Liu V.W.S., Xue W., Tsang P.C.K., Cheung A.N.Y. and Ngan H.Y.S., The Increase Of Mitochondrial Dna Content In Endometrial Adenocarcinoma Cells: A Quantitative Study Using Laser-captured Microdissected Tissues, Gynecologic Oncology. 2005, 98: 104-110.


Researcher : Wang Y

List of Research Outputs

Wang Y., Liu V.W.S., Tsang P.C.K., Chiu P.M., Cheung A.N.Y., Khoo U.S., Nagley P. and Ngan H.Y.S., Microsatellite instability in mitochondrial genome of common female cancers, International Journal of Gynecological Cancer. 2006, Suppl 1: 259-266.
Wang Y., Liu V.W.S., Xue W., Tsang P.C.K., Cheung A.N.Y. and Ngan H.Y.S., The Increase Of Mitochondrial Dna Content In Endometrial Adenocarcinoma Cells: A Quantitative Study Using Laser-captured Microdissected Tissues, Gynecologic Oncology. 2005, 98: 104-110.


Researcher : Wong KY

List of Research Outputs

Leung K.Y., Chan K.K.L., Ngai C.S.W., Leung W.C., Lee C.P., Wong K.Y. and Tang M.H.Y., Endoscopic laser therapy for twin transfusion syndrome, The XIXth Asian and Oceanic Congress of Obstetrics and Gynecology, COEX Convention and Exhibition Center, Seoul, Korea, October 1-5, 2005.


Researcher : Wong RLC

List of Research Outputs

Tam K.F., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Ethnicity is a factor to be considered before dose planning in ovarian cancer patients to be treated with topotecan, International Journal of Gynecological Cancer. 2006, 16: 135-139.
Tam K.F., Chau M.T., Chan Y.M., Ng T.Y., Wong R.L.C. and Ngan H.Y.S., Hepatic arteriogram for gestational trophoblastic tumor: Is it useful? , Cancer Investigation. 2005, 23: 677-682.


Researcher : Xu J

List of Research Outputs

Lee C.K.F., Xu J., Lee C.Y.L. and Yeung W.S.B., Demilune cell and parotid protein from murine oviductal epithelium stimulates preimplantation embryo development, Endocrinology. 2006, 147(1): 79-87.


Researcher : Yeung WSB

Project Title:Functional studies of VCY2 and VCY2 interacting protein (VCY2IP-1)
Investigator(s):Yeung WSB, Tam PC, Tse JYM, Huang J
Department:Obstetrics & Gynaecology
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2003
Abstract:
N/A


Project Title:Regulation of the complement C3 expression in mouse
Investigator(s):Yeung WSB, Lee CYL, Lee CKF
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Completion Date:10/2005
Abstract:
To study the regulation of embryotrophic C3 in the mouse oviduct.


Project Title:The role of glycodelins on capacitation and acrosome reaction of human spermatozoa
Investigator(s):Yeung WSB
Department:Obstetrics & Gynaecology
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2005
Completion Date:06/2006
Abstract:
N/A


Project Title:The mechanisms of action of glycodelin-A on zona pellucida induced acrosome reaction
Investigator(s):Yeung WSB, Chiu CN, Lee CKF
Department:Obstetrics & Gynaecology
Source(s) of Funding:Small Project Funding
Start Date:12/2005
Abstract:
Glycodelin is a glycoprotein belonging to the lipocalin family. It has 3 reported isoforms; namely amniotic fluid-derived glycodelin-A, seminal plasma-derived glycodelin-S and follicular fluid-derived glycodelin-F (Seppala et al., 2002; Chiu et al., 2003). The isoforms of glycodelin have the same protein core but differ in their glycosylation. Glycodelin-A is the first endogenous glycoprotein found to inhibit spermatozoa-zona pellucida binding (Oehninger et al., 1995). Its absence in the endometrium in the periovulatory period may be related to the presence of a fertilization window (Seppala et al., 1998). Glycodelin-A is produced by the oviduct tissue and is also present in the follicular fluid (Chiu PCN and Yeung WSB, unpublished data). Thus the spermatozoa are likely to come in contact with glycodelin-A in the oviduct before they meet the oocyte. We hypothesize that glycodelin-A has effects on spermatozoa other than its action on spermatozoa-zona pellucida interaction. Indeed, our preliminary data show that glycodelin-A stimulates zona-pellucida-induced acrosome reaction of human spermatozoa, though the molecule does not affect spontaneous acrosome reaction. Acrosome reaction is a critical event in fertilization and is induced by the zona pellucida of oocyte. It allows the spermatozoa to release acrosomal enzymes, and thereby facilitating the penetration of the spermatozoa through the zona pellucida. Our observation suggests that glycodelin-A primes the spermatozoa for this important event of fertilization. The objective of this proposal is to study the intracellular mechanisms in human spermatozoa leading to the priming action of glycodelin-A on zona pellucida-induced acrosome reaction. A priming effect of progesterone on acrosome reaction had been reported in mouse (Roldan et al., 1994) and guinea-pig (Shi et al. 2005) and is regulated by intracellular calcium concentration, phospholipase and/or cAMP/PKA pathway. References Chiu PCN, Koistinen R, Koistinen H, Seppala M, Lee KF, Yeung WSB (2003) Biol Reprod, 69, 365-72. Oehninger S, Coddington CC, Hodgen GD, Seppala M (1995) Fertil Steril, 63, 377-83. Oehninger S, Franken DR, Sayed E, Barroso G, Kolm P (2000) Hum Reprod Update 6, 160-8. Seppala M, Taylor RN, Koistinen H, Koistinen R, Milgrom E (2002) Endocr Rev 23, 401-430.. Seppala M, Koistinen H, Koistinen R, Mandelin E, Oehninger S, Clark GF, Dell A, Morris HR. (1998) Hum Reprod 13 (suppl 3), 262-70. Roldan ER, Murase T, Shi QX (1994) Science, 266(5190), 1578-81. Shi QX, Chen WY, Yuan YY, Mao LZ, Yu SQ, Chen AJ, Ni Y, Roldan ER (2005) J Cell Physiol.[Epub ahead of print].


List of Research Outputs

Ault K., Neff T., Kelly K., Yeung W.S.B., Tsao S.W. and Lee C.K.F., Chemokine and cytokine gene expression in immortalized human oviduct epithelial cell infected with chlamydia trachomatis, Annual Scientific Meeting and Symposium of the Infectious Disease Society for Obstetrics and Gynecology (IDSOG), South Carolina, USA, August 18-20, 2005.
Chan C.C.W., Ng E.H.Y., Tang F.O.S., Yeung W.S.B., Lau E.Y.L. and Ho P.C., A prospective, randomized, double-blind study to compare two doses of recombinant human chorionic gonadotropin in inducing final oocyte maturity and the hormonal profile during the luteal phase, The Journal of Clinical Endocrinology and Metabolism. 2005, 90(7): 3933-3938.
Chan C.C.W., Koo M.W.L., Ng E.H.Y., Tang F.O.S., Yeung W.S.B. and Ho P.C., Effects of Chinese green tea on weight, and hormonal and biochemical profiles in obese patients with polycystic ovary syndrome - A randomized placebo-controlled trial, Journal of The Society for Gynecology Investigation. 2006, 13(1): 64-68.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Content And 4977 bp Deletion In Unselected And Unfertilized Oocytes, Molecular Human Reproduction. 2006, 11: 843-846.
Chan C.C.W., Liu V.W.S., Lau E.Y.L., Yeung W.S.B., Ng E.H.Y. and Ho P.C., Mitochondrial DNA Deletion In Granulosa And Cumulus Oophorus Cells, Fertility and Sterility. 2006, 85: 780-782.
Chiu C.N., Chung M.K., Lee C.K.F., Ho P.C. and Yeung W.S.B., Adrenomedullin effects on sperm functions. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Chiu C.N., Chung M.K., Tsang H.Y., Koistinen R., Koistinen H., Seppala M., Lee C.K.F. and Yeung W.S.B., Glycodelin-S in human seminal plasma reduces cholesterol efflux and inhibits capacitation of spermatozoa, The Journal of Biological Chemistry. 2005, 280(27): 25580-25589.
Chung M.K., Chiu C.N., Lee C.K.F., Ho P.C. and Yeung W.S.B., A role for human sperm surface fucosyltransferase in glycodelin binding., International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006, P.10.
Chung M.K., Chiu C.N., Lee C.K.F., Yeung W.S.B. and Ho P.C., Biological and biochemical characterization of the glycodelin isoform from human cumulus cell matrix. P5.38, Frontiers in Biomedical Research, HKU 2005, The University of Hong Kong. 3 Dec, Hong Kong.. 2005.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, Prenatal Diagnosis. 2006, 26: 510-513.
Hui P.W., Tang M.H.Y., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Nuchal translucency in dichorionic twins conceived after assisted reproduction, The 13th International Conference on Prenatal Diagnosis and Therapy, Kyoto, Japan, 28 - 31 May. 2006.
Kan A., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Perifollicular vascularity in poor ovarian responders during IVF, Human Reproduction. 2006, 21(6): 1539-1544.
Lee C.K.F., Xu J., Lee C.Y.L. and Yeung W.S.B., Demilune cell and parotid protein from murine oviductal epithelium stimulates preimplantation embryo development, Endocrinology. 2006, 147(1): 79-87.
Lee C.K.F. and Yeung W.S.B., Gamete/Embryo-Oviduct Interactions: Implications on in vitro culture. , British Andrology Society Annual Meeting. 17-18 Nov, Sheffield, UK.. 2005, P.10.
Lee C.K.F., Leung K.Y., Chung M.K., Lee C.Y.L., Chow J.F.C. and Yeung W.S.B., Phospholipid transfer protein (PLTP) mRNA expression is stimulated by developing embryos in the oviduct, Journal of Cellular Biochemistry. 2005, 95: 740-749.
Lee C.K.F., Cheung W.K.L., Chung M.K., Chiu C.N., Yeung W.S.B. and Ng E.H.Y., Proteomic profiling of the uterine flushing from natural and stimulated cycles during peri-implantation period., Society for the Study of Reproduction -38th Annual Meeting. 24-27 Jul, Quebec, Canada. . 2005.
Lee N.P., Leung K.W., Wo J.Y., Tam P.C., Yeung W.S.B. and Luk J.M.C., Blockage of testicular connexins induced apoptosis in rat seminiferous epithelium, Apoptosis. 2006, 11(7): 1215-1229.
Lee P.Y., Tong M.K., Leung P.L., Chan V.W.M., Leung S.Y.L., Tam P.C., Chan K.W., Lee C.K.F., Yeung W.S.B. and Luk J.M.C., Kidney claudin-19: Localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease, FEBS Letters. 2006, 580(3): 923-31.
Ling L., Lee C.Y.L., Lee C.K.F., Tsao G.S.W., Yeung W.S.B. and Kan F.W.K., Expression of human oviductin in an immortalized human oviductal cell line, Fertility and Sterility. 2005, 84 Supplement 2: 1095-1103.
Ling L., Lee C.Y.L., Lee C.K.F., Yeung W.S.B. and Kan F.W.K., Study of gene and protein expression of oviductin in an immortalized human oviductal cell line, 38th Annual Meeting, Society for The Study of Reproduction, Quebec, Canada, July 24-27, 2005.
Makkar G., Ng E.H.Y., Yeung W.S.B. and Ho P.C., Reduced expression of interleukin-11 and interleukin-6 in the peri-implantation endometrium of excessive ovarian responders during in-vitro fertilization treatment, Journal of Clinical Endocrinology and Metabolism. 2006, 91(8): 3181-3188.
Ng E.H.Y., Chan C.C.W., Yeung W.S.B. and Ho P.C., Comparison of ovarian stromal flow between fertile women with normal ovaries and infertile women with polycystic ovary syndrome, The 6th International Scientific Meeting, Cairo, Egypt, September 27-30, 2005.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Factors affecting endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound during IVF treatment, Human Reproduction. 2006, 21(4): 1062-1069.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., Relationship between uterine blood flow and endometrial and subendometrial blood flows during stimulated and natural cycles, Fertility and Sterility. 2006, 85(3): 721-727.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flow measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during frozen-thawed embryo transfer cycles, Human Reproduction. 2006, 21(6): 1612-1617.
Ng E.H.Y., Chan C.C.W., Tang F.O.S., Yeung W.S.B. and Ho P.C., The role of endometrial and subendometrial blood flows measured by three-dimensional power Doppler ultrasound in the prediction of pregnancy during IVF treatment, Human Reproduction. 2006, 21(1): 164-170.
Ng E.H.Y., Yeung W.S.B. and Ho P.C., The significance of antral follicle count in controlled ovarian stimulation and intrauterine insemination, Journal of Assisted Reproduction and Genetics. 2005, 22: 323-328.
Ngan E.S.W., Lee K.Y., Yeung W.S.B., Ngan H.Y.S., Ng E.H.Y. and Ho P.C., Endocrine Gland-Derived Vascular Endothelial Growth Factor is expressed in human peri-implantation endometrium, but not in endometrial carcinoma, Endocrinology. 2006, 147: 88-95.
Seppala M., Koistinen R., Koistinen H., Chiu C.N. and Yeung W.S.B., Glycodelin:A Lipocalin with Diverse Glycoform-Dependent Actions, In: Akerström B, N. Borregaard, D.R. Flower Edward, J.P. Salier, Lipocalins. USA, Landes Bioscience, 2006, 121-130.
Siu S.O., Cox D., Lee C.K.F., Yeung W.S.B. and Chu I.K., Characterization of N-linked Glycosylation sites and glycams of glycodelins by means of tandem mass spectrometry, 54th American Society for Mass Spectrometry Conference, Seattle, USA. 2006.
Siu S.O., Cox D., Lee C.K.F., Yeung W.S.B. and Chu I.K., Characterization of N-linked glycosylation sites and glycans of glycodelins by means of tandem mass spectrometry , Poster Presentation in Proceedings of 54th ASMS Conference on Mass Spectrometry and Allied Topies, Seattle, Washington State, U.S.A., May 28 - June 1, 2006 . 2006, Poster No. 394.
Siu S.O., Cox O., Lee C.K.F., Yeung W.S.B. and Chu I.K., Characterization of N-linked glycosylation sites and glycans of glycodelins by means of tandem mass spectrometry, Poster Presentation in 13rd Symposium on Chemistry Postgraduate Research in Hong Kong . 2006, A19.
Yeung W.S.B., Lee C.Y.L. and Lee C.K.F., Coculture versus sequential culture in assisted reproduction., In: Yeung WS, Lee YL, Lee KF , The XIXth Asian and Oceanic Congress of Obstetrics and Gynaecology. S4C-3. 1-5 Oct, Seoul, Korea.. 2005.
Yeung W.S.B., Lee C.K.F. and Lee C.Y.L., Complement protein-3: an oviductal paracrine factor regulating embryo development, 12th World Congress of Gynecological Endocrinology, Florence, Italy, March 2-5, 2006. 2006.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Glycodelin: A molecule with multi-functions on spermatozoa. , International Congress on Gamete Biology: Emerging Frontiers in Fertility and Contraceptive Development. Feb 22-25, New Delhi, India.. 2006.
Yeung W.S.B., Glycodelin: a molecule with multi-functions on spermatozoa , 10th Meeting of Chinese Reproductive Biologists, November 1-3, 2005, Kunming, China. 2005.
Yeung W.S.B., Lee C.K.F. and Lee C.Y.L., Oviductal embryotrophic factors, 5th International Conference on Reproductive Endocrinology, 9-12 September 2005 Beijing, China.. 2005.
Yeung W.S.B., Lee C.K.F., Koistinen R., Koistinen H., Seppala M., Ho P.C. and Chiu C.N., Roles of glycodelin in modulating sperm function, Molecular and Cellular Endocrinology. 2006, 250: 149-56.


Researcher : Yu YM

List of Research Outputs

Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes In The Mitochondrial Content Of Cervical Cancer Cells Under Stress, The 3rd Asain Society for Mitochondrial Research and Medicine December 14-17, 2005, Tokyo, Japan. 2005.
Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes in mitochondrial content of cervical cancer cells under stress, The 3rd Conference of the Asian Society for Mitochondrial Research and Medcine , December 14-17, 2005, Tokyo, Japan. 2005.


Researcher : Yu YM

List of Research Outputs

Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes In The Mitochondrial Content Of Cervical Cancer Cells Under Stress, The 3rd Asain Society for Mitochondrial Research and Medicine December 14-17, 2005, Tokyo, Japan. 2005.
Liu V.W.S., Yu Y.M. and Ngan H.Y.S., Changes in mitochondrial content of cervical cancer cells under stress, The 3rd Conference of the Asian Society for Mitochondrial Research and Medcine , December 14-17, 2005, Tokyo, Japan. 2005.


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