DEPT OF PHYSIOLOGY

Researcher : Ballard HJ

Project Title:

Interactions between vasoconstrictor and vasodilator mechanisms in the skeletal muscle vascular response to systemic hypoxia

Investigator(s):

Ballard HJ

Department:

Physiology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

02/2004

 

Abstract:

To investigate the roles of the baroreflex, the central chemoreflex and adaptation of the peripheral chemoreflex in the variation of sympathetic outflow to the muscle during hypoxia, and the mechanism by which sympathetic nerve activation stimulates the formation of interstitial vasodilator metabolites; to investigate the roles of A1 and A2 adenosine receptors in the vascular response to sustained hypoxia, and determine whether the receptors are located on endothelium or vascular smooth muscle; to investigate the mechanisms of the interactions between the release and actions of the vasodilator substances in hypoxia; to investigate the properties and control of the specific isoenzyme of cytosolic 5' -nucleotidase isolated from skeletal muscle vascular endothelial cells.

 

Project Title:

Sources and actions of adenosine in exercise vasodilation of rat red or white muscle

Investigator(s):

Ballard HJ

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2004

 

Abstract:

To determine the venous and interstitial concentrations of adenosine in red and white muscles, and evaluate the contribution of A1 and A2 receptors to the vasodilation using appropriate blockers.

 

Project Title:

The role of lactic acid in the regulation of ATP efflux from skeletal muscle.

Investigator(s):

Ballard HJ

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

10/2005

 

Abstract:

Skeletal muscle blood flow increases by as much as 2000% during muscle contractions. It is known that the interstitial ATP concentration increases during muscle contractions, and that this ATP is converted extracellularly to adenosine, accounting for a large part of the active hyperaemia. However, it is not known how the muscle contractions give rise to the increase in interstitial ATP. Certainly ATP can not be categorised as a “metabolic waste product”: thus, this mechanism of blood flow regulation does not fit into our existing concept for metabolic autoregulation of blood flow. Previous studies have shown that either muscle contractions (which give rise to the formation of endogenous lactic acid), or the infusion of exogenous lactic acid, cause a decrease in the muscle pH and increase both the interstitial and venous adenosine: there was a strong correlation between the depression of muscle pH and the appearance of adenosine in the venous blood, with points from both the acid infusion experiments and the muscle contraction experiments falling on a single straight line. Blockade of the cellular uptake of infused lactic acid with a-cyano-4-hydroxycinnamic acid abolished the increase in adenosine output from the muscle. Taken together, these data suggest that it is the increase in intracellular lactic acid during muscle contractions that stimulates the efflux of ATP from the muscle cells, which in turn leads to the formation of adenosine extracellularly. We have performed a small number of pilot experiments, which indicated that arterial infusion of exogenous lactic acid did, indeed, increase the interstitial ATP concentration of muscle. However, the mechanism by which lactic acid stimulates the ATP efflux, and the route by which ATP leaves the cell, are unknown, although, since ATP is a charged molecule, it is highly likely that a specific carrier is involved. The predominant mechanism of ATP transport across skeletal muscle cell membranes has not been investigated previously. A number of transporters have been identified on the surface membranes of other cell types which permit the efflux of ATP from those cells: these include members of the ATP-binding cassette (ABC) family of proteins, such as the multidrug resistance-1 protein, the cystic fibrosis transmembrane conductance regulator, and P-glycoprotein, as well as other electrodiffusional pathways, such as the cAMP-stimulated anion-selective channel. Whether any of these transporters are involved in ATP efflux from skeletal muscle cells remains to be investigated. Furthermore, the mitochondrial ATP transporter or the nucleoside transporter may play a role in ATP efflux, which should also be investigated. The objectives of this study, therefore, are to determine: 1. the influence of infused lactic acid on the skeletal muscle interstitial ATP concentration 2. whether lactic acid acts at an intra- or extracellular site to stimulate ATP efflux 3. whether ATP leaves the skeletal muscle cell through one of the known ATP transporter proteins

 

Project Title:

Adenosine formation in vascular endothelial cells

Investigator(s):

Ballard HJ

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

10/2006

 

Abstract:

The main objective of the study is to determine whether vascular endothelial cells are capable of forming adenosine intracellularly. Adenosine acts on the skeletal muscle vascular bed to induce vasodilation during both muscle contractions and systemic hypoxia. However, during muscle contractions, adenosine acts on A2 receptors on the vascular smooth muscle cells, whereas, during hypoxia, it acts mainly on A1 receptors, which are thought to be on the vascular endothelium. Our previous studies have suggested that the site of formation of adenosine may be different during muscle contractions and hypoxia: we used interstitial microdialysis to show that the concentrations of ATP, ADP, AMP and adenosine were all elevated in the interstitial space during muscle contractions. Studies of the properties of the adenosine-metabolising enzymes in a crude homogenate of muscle showed that cytosolic 5’-nucleotidase had a much lower activity than ecto 5’ -nucleotidase, confirming that adenosine was formed extracellularly from extracellular nucleotides during muscle contractions. During hypoxia, however, we found that venous adenosine was elevated, but the venous adenine nucleotides and the interstitial adenosine and adenine nucleotides remained unchanged. This suggests that, during hypoxia, adenosine may be formed by vascular endothelial cells, and that it may be formed intracellularly. If this is so, it could explain how adenosine can act on different receptors in hypoxia from those during muscle contractions. It has been shown that the expression of 5’-nucleotidase declines very rapidly in cultured cells, and so, this question can only be addressed using freshly-isolated endothelial cells. The properties of the adenosine-metabolising enzymes of fresh endothelial cells have not been studied previously. The specific aims of this project are to: 1. Isolate vascular endothelial cells from the skeletal muscle circulation 2. Examine the properties of the isolated cells to ensure the purity of the endothelial preparation 3. Separate the cytosolic and ecto forms of 5’-nucleotidase from a homogenate of freshly isolated vascular endothelial cells 4. Study the properties of the cytosolic and ecto forms of 5’-nucleotidase

 

 

Researcher : Bourreau JP

Project Title:

Role of cyclooxygenase in the ventricular effects of adrenomedullin: does prostacyclin contribute towards the cardiodepression in the late hypodynamic phase of septic shock?

Investigator(s):

Bourreau JP

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2003

 

Abstract:

To measure the levels of PGI2 by quantifying its stable hydrolysis product, 6-keto PGF1[alpha] by EIA after stimulation with ADM and at various time-points corresponding to the biphasic inotropic effects of ADM, and to mesure the PGI2 levels in our animal model of terminal septic shock; to observe the signal transduction pathway involved in the ventricular effects of ADM with respect to PGI2.

 

Project Title:

Endothelim-dependent hyperpolarization and relaxation of vascular smooth muscle: influence of the mode of excitation-coupling of vasoconstrictors

Investigator(s):

Bourreau JP

Department:

Physiology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

02/2005

 

Abstract:

To test the hypothesis that expression of endothelium-dependent hyperpolarization-induced relaxation in a given vascular bed depends on the vasoconstrictor's coupling mode. We aim in the project at establishing controlled experimental conditions that will optimise the expression of endothelium-dependent hyperpolarization-induced relaxation and thus optimize the investigation of its mechanism(s).

 

Project Title:

Switch in G-protein coupling. Role of intracellular cAMP

Investigator(s):

Bourreau JP

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

01/2006

 

Abstract:

In our animal model of septic shock, we were able to identify the overproduction of the vasoactive peptide adrenomedullin as a key factor responsible for depression of ventricular function during the hypodynamic phase of shock (Am.J.Physiol., Heart Circ.Physiol. 286: H1034-H1042, 2004).Physiologically, this peptide is a positive inotropic substance that increases the force of contraction via a beta adrenergic-like effect; i.e., by raising the intracellular cAMP concentration. This triggers PKA-dependent phosphorylation of key protein targets (L-type calcium channels, phospholamban, TnC…), which results in positive inotropy and lusitropy.It was thus difficult to understand why a substance that, as often claimed in the litterature, should function to overcome situations where cardiac function is compromised (such as septic shock), failed to do so but rather contributed significantly to the progression of ventricular depression.We have also gathered data showing that the negative inotropic effect of adrenomedullin develops slowly and is dependent on the concomittent expression of the inducible form of cyclooxygenase, COX2 (Am J Physiol Heart Circ Physiol. 2004 Mar;286(3):H1034-42). Moreover, our recent findings (under revision, Am J.Physiol.) show clearly that this negative inotropic effect of adrenomedullin in the presence of COX 2 induction is pertussis toxin-sensitive, suggesting a switch in G-protein coupling from Gs to Gi. This negative inotropic effect of adrenomedullin is also sensitive to inhibition of PKA, which suggests that PKA-dependent phosphorylation of specific target(s) is responsible for the switch, implicating a role for cytosolic cAMP in this phenomenon.Therefore, the model we propose assumes that it is an uncontrolled increase in cAMP, due to ADM and prostaglandins (most probably prostacyclin) stimulation that initiate a sequence of events leading to a switch in G-protein coupling between adrenomedullin receptor and cellular response. This cAMP-dependent switch would thus be the trigger for the depressant effect of adrenomedullin on the ventricule during septic shock.The goal of this project is to test this hypothesis by quantifying cAMP in rat ventricular myocytes in conditions were adrenomedullin has a positive and a negative inotropic effect, and to confirm that pharmacological manipulations of these myocytes that inhibit the effects of adrenomedullin ultimately result in a decrease production of cAMP.

 

Project Title:

Ventricular excitation-contraction coupling during septic shock: Influence of adrenomedullin

Investigator(s):

Bourreau JP

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

01/2007

 

Abstract:

To quantify the changes in cellular cAMP occurring in ventricular myocytes isolated from rats in the hypo-dynamic phase of septic shock; to quantify the effect of adrenomedullin receptor blockade (qnd/or COX II inhibition) on the cAMP levels in myocytes isolated fron shocked rats; to document the cellular pathways involved, leading to depression of ventricular myocytes twitch contraction; to identify the processes of excitation-contraction coupling in ventricular myocytes altered during the hypo-dynamic phase of septic shock, and document the implication of adrenomedullin in these changes.

 

List of Research Outputs

 

Zhang X., Li G.R. and Bourreau J.P., The effect of adrenomedullin on the L-type calcium current in myocytes from septic shock rats: Signaling pathway. , Am J Physiol Heart Circ Physiol. 2007, 293(5): H2888-93.

 

Researcher : Bruce IC

Project Title:

Biomedical writing for postgraduate students whose mother tongue is Chinese: a novel course based on interactive multimedia

Investigator(s):

Bruce IC

Department:

Physiology

Source(s) of Funding:

Teaching Development Grants

Start Date:

02/1999

 

Abstract:

Two tasks need to be accomplished. Firstly, how best to teach and learn the art and craft of creating a manuscript suitable for submission to an international journal. Secondly, how to develop a teaching tool that is sensitive to the specific difficulties encountered by individuals whose usual channels of communication are Chinese. The unifying strategy is to create and interactive web site that can be used, in combination with face-to-face teaching, to develop the desired writing skills and attitudes.

 

 

Researcher : Chan YS

Project Title:

Sensitive period for topographic spatial representation in the rat otolith system

Investigator(s):

Chan YS

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

11/2003

 

Abstract:

To determine whether the functional organization of a gravity-related spatial reference in the postnatal inferior olive is perturbed by perinatal blockade of the vestibular nucleus; to determine whether the spatial coding capacity of adult neurons are perturbed well after an early postnatal blockade becomes ineffective; to examine if the pharmacological manipulations applied later in the postnatal period have any effect in the mature brain.

 

Project Title:

The role of central glial cells in the pathogenesis of Parkinson's Disease

Investigator(s):

Chan YS

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2003

 

Abstract:

To employ immuno-hybridization histochemical methods to map the spatiotemporal distribution of different activated glial cells during the development of PD as induced by systemic administration of a neurotoxicant, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP).

 

Project Title:

Development of commissural inhibition in the vestibular system

Investigator(s):

Chan YS

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

01/2005

 

Abstract:

To map the postnatal maturation time and the location of canal-related neurons in the vestibulo-olivary pathway; to document the location and maturation profile of inhibitory neuronal correlates in the commissural otolith pathway; to examine the developmental change of inhibitory postsynaptic current (IPSC) at central GABAergic synapses in brainstem slices containing the vestibular nucleus.

 

Project Title:

Development of glutamate transmission and frequency responsiveness in central vestibular neurons

Investigator(s):

Chan YS

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

12/2005

 

Abstract:

To examine the postnatal change of excitatory postsynaptic current at glutamate synapses in the vestibular nucleus; to investigate the frequency responsiveness of neuronal subgroups within the medial vestibular nucleus.

 

Project Title:

Functional maturation of excitatory and inhibitory synaptic receptors in canal-and otolith-related neurons of the inferior olive

Investigator(s):

Chan YS

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

12/2006

 

Abstract:

To examine developmental change of excitatory postsynaptic currents at glutamate synapses on canal- and otolith-related inferior olivary neurons; to examine developmental change of inhibitory postsynaptic currents at GABAergic synapses on canal- and otolith-related inferior olivary neurons.

 

List of Research Outputs

 

Ao Q., Wang A.J., Chen G.Q., Zuo H.C., Zhang X.F., Fung C.K., Tsui Y., Shum D.K.Y. and Chan Y.S., Fabrication and characterization of multi-channel chitosan nerve conduit and its potential application, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 30. March 1-2. 2008.

 

Chan C., Liu H., Chan Y.S. and Shum D.K.Y., 4-O-sulfated Chondroitins Contribute to Axon-restrictive Property in Schwann cell-as-trocyte Encounters, 12th Research Postgraduate Symposium, December 12 & 14, 2007.

 

Chan C.F., Leung A.Y.H., Chan Y.S. and Cheung R.T.F., Central administration of granulocyte- colony stimulating factor in a mice middle cerebral artery occlusion stroke model reduces infarct volume and improves functional outcome, Soc. Neuroscience Abstract (U.S.A.): 598.1. 2007.

 

Chan Y.S., Animal experimentation and ethical issues, 7th IBRO World Congress of Neuroscience. Melbourne, Australia. July 12-17. 2007.

 

Chan Y.S. and He J., Corticofugal synchronization leads to c-fos expression in the auditory thalamus. Neuroscience Bulletin 23 (Suppl 1): 11., 7th Biennial Conference of Chinese Society for Neuroscience. Hangzhou, China. Oct. 24-28. 2007.

 

Chan Y.S., Tse Y.C., Lai S.K., Lai C.H. and Yung W.H., Developmental changes in excitatory and inhibitory transmission at central vestibular synapses of rats. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007.

 

Chan Y.S., Developmental refinement of local glutamate circuit in spatial recognition, International Symposium on Integration of Sensory Information. Beijing. May 15-16. 2008.

 

Chan Y.S., Developmental refinement of spatial representation and sensory-motor interaction, 2nd International Meeting of Molecular and Cellular Cognition. Yokohama, Japan. Sept. 8-9 . 2007.

 

Chan Y.S., Neural circuit refinement for vestibular recognition of gravity-related spatial orientation, Proceedings of Physiology Symposium: 16. Taipei, March 30-31. 2007.

 

Chan Y.S., Neurochemistry International, 2008.

 

Chan Y.S., Postnatal representation of spatial orientation in the brain. Proceedings of International Symposium on Consciousness, Brain Science, and Religion: 38-39. Hsinchuang, Taiwan. Oct 12-14. 2007.

 

Chan Y.S., Postnatal representation of spatial orientation in the brain, International Symposium on Consciousness, Brain Science and Religion. Hsinchuang, Taiwan. Oct. 12-14. 2007.

 

Chan Y.S., Lai C.H., Lai S.K., Li C., Ma C.W. and Tse Y.C., The differential role of glutamate receptors in postnatal spatial recognition. Journal of Neurochemistry, 106 Suppl: 6, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, China. June 24-27. 2008.

 

Chan Y.S., The differential role of glutamate receptors in postnatal spatial recognition, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai. June 24-27. 2008.

 

Cheah K.S.E., Szeto Y.Y., Au Y.K., Wynn S., Geng G., Chan Y.S., Chan W.Y., Cheung K.M.C. and Fritzsch B., Context dependent impact of the Y440X campomelic dysplasia Sox9 mutation, ACGA-HKSMG International Conference on Genetic and Genomic Medicine, Hong Kong. 8-11 June 2008.

 

Cheah K.S.E., Szeto Y.Y., Au Y.K., Chan Y.S., Lovell-Badge R. and Fritzsch B., Hearing and balance defects in a mouse model of campomelic dysplasia, Gordon Research Conference on Craniofacial Morphogenesis and Tissue Regeneration. Lucca, Italy. Feb 10-15 . 2008.

 

Chen L., Yung K.K.L., Chan Y.S. and Yung W.H., 5-HT excites globus pallidus neurons by multiple receptor mechanisms. , Neuroscience. 2008, 151: 439-451.

 

Chen L.W., Wang Y.Q., Wei L.C., Shi M. and Chan Y.S., Chinese herbs and herbal extracts for neuroprotection of dopaminergic neurons and potential therapeutic treatment of Parkinson’s disease. , CNS & Neurological Disorders - Drug Targets. 2007, 6: 273-281.

 

Chung E.K.Y., Chen L.W., Chan Y.S. and Yung K.K.L., Up-regulation in the expression of vesicular glutamate transporter 3 in substantia nigra but not in striatum of 6-hydroxydopamine-lesioned rats. , NeuroSignals. 2007, 15: 238-248.

 

Fung C.K., Shum D.K.Y. and Chan Y.S., Derivation of Schwann cell from mammalian skin for use in axonal degeneration, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 16. March 1-2. 2008.

 

Gao J., Lee T.M.C., Chan Y.S., Chu L.W. and Cheung R.T.F., Cognitive assessments of prospective memory and arrowhead tasks in patients with mild Alzheimer’s disease and in healthy elderly, Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 44. March 1-2. Hong Kong, 2008.

 

He J., Liu C.H., Guo Y.P., Meng X.K. and Chan Y.S., The spindle oscillations in the thalamic reticular nucleus, auditory thalamus, and cortex, Soc. Neuroscience Abstract (U.S.A.): 881.2. 2007.

 

Lai C.H., Yiu C.N.S., Tse Y.C. and Chan Y.S., Functional maturation of vestibular-related brainstem neurons: Postsynaptic currents and responsiveness to horizontal rotational-/translational head movements, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, # POS-SAT-280.

 

Lai C.H., Yiu C.N.S. and Chan Y.S., Postnatal development of canal-related brainstem neurons responsive to three-dimensional rotations in rats, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 23. March 1-2. 2008.

 

Lai C.H., Yiu C.N.S. and Chan Y.S., Postnatal development of canal-related brainstem neurons responsive to vertical head rotation in rats. Neuroscience Bulletin 23: 83, 7th Biennial Conference of Chinese Society for Neuroscience. Hangzhou, China. Oct. 24-28. 2007.

 

Lai S.K., Lai C.H., Wong T.P., Yung W.H. and Chan Y.S., Contribution of glutamate receptors to the maturation of central vestibular neurons in rats, Soc. Neuroscience Abstract (U.S.A.): 180.18. San Diego. 2007.

 

Lai S.K., Wong T.P., Yung W.H., Shum D.K.Y. and Chan Y.S., Maturation profile of ionotropic glutamate receptors in central vestibular neurons of rats. Journal of Neurochemistry 106 Suppl: 43-44. , 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, June 24-26. 2008.

 

Lai S.K., Wong T.P., Yung W.H. and Chan Y.S., Postnatal development of ionotropic glutamate receptors in central vestibular neurons of rats. , Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 24. March 1-2. 2008.

 

Lai S.K., Wong T.P., Yung W.H. and Chan Y.S., Postnatal modification in glutamatergic synapses within the rat vestibular nucleus, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, #POS-SAT-281.

 

Li M., Chan Y.S. and Shum D.K.Y., Chondroitin Sulfate Moieties Restrict Migration of Cranial Motor Neurons in the Developing Hindbrain of Rats, 12th Research Postgraduate Symposium, December 12 & 14, 2007.

 

Li M., Chan Y.S. and Shum D.K.Y., The restrictive role of chondroitin sulfate moieties on the migration of cranial motor neurons in rat hindbrain development, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 25. March 1-2. 2008.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Differential involvement of perineuronal net components in functional modifications of rat vesticular nucelar neurons, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 3. March 1-2. 2008.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Modifications in perineuronal nets of rat vestibular nuclear neurons during postnatal development and in injury, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12 . 2007, 58: S98.

 

Ng K.P., Lai C.H., Tse Y.C. and Chan Y.S., Thalamic neurons detect both rotational and translational motions in the horizontal plane. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007, 58.

 

Ng K.P., Lau P.Y.P., Lai C.H. and Chan Y.S., Topographic distribution of thalamic neurons responsive to angular and linear head movements. , Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 26. March 1-2. 2008.

 

Shea G.K.H., Tsui Y., Chan Y.S. and Shum D.K.Y., Functional study of stem-cell-derived Schwann cells using an in-vitro dorsal root ganglia model. Abstracts of Third International Symposium on Healthy Aging, Improving the Health of an Aging Population. 2008, 17.

 

Shum D.K.Y., Chan C., Liu H.Y., Chau C.H. and Chan Y.S., Addressing the pericellular matrix of reactive astrocytes. , Abstracts of Croucher Advanced Study Institute: Innovative Therapies of Movement Disorders, D12. HK. Nov. 27-30. 2007.

 

Szeto Y.Y., Au Y.K., Wynn S., Chan Y.S., Lovell-Badge R., Chan W.Y., Fritzsch B. and Cheah K.S.E., A mouse model for hearing and balance defects in campomelic dysplasia, 6th Molecular Biology of Hearing & Deafness Conference. Hinxton, UK. 14 July . 2007.

 

Tam K.W., Li R.A., Chan Y.S. and Shum D.K.Y., Cloning, recombinant expression and digestion product characterization of chondroitinse ABC I and II, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, #POS-SUN-050.

 

Tam K.W., Li R.A., Chan Y.S. and Shum D.K.Y., Effects of oligosaccharide products on activity of chondroitin sulphate ABC lyase I – Towards improving the efficacy of the enzyme for in vivo treatment, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 4. March 1-2. 2008.

 

Tse Y.C., Lai C.H., Lai S.K., Liu J.X., Yung K.K.L., Shum D.K.Y. and Chan Y.S., Developmental expression of NMDA and AMPA receptor subunits in vestibular nuclear neurons that encode gravity-related horizontal orientations. , Journal of Comparative Neurology. 2008, 508: 343-364.

 

Tsui Y., Shea G.K.H., Chan Y.S. and Shum D.K.Y., Schwann cells derived from bone marrow stromal cells – in vitro study with dorsal root ganglia and in vitro study with peripheral nerve conduits, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 19. March 1-2. 2008.

 

Tsui Y.P., Shea G.K.H., Chan Y.S. and Shum D.K.Y., Preparations of Chitosan Support for Cultures of Schwann Cells and Transdifferentiated Mesenchymal Stem Cells, 12th Research Postgraduate Symposium, December 12 & 14, 2007.

 

Wang N., Zhang Z., Chan Y.S. and He J., Feed-forward modulation of the thalamocortical projections on different layers of the auditory cortex, Soc. Neuroscience Abstract (U.S.A.): 278.6. 2007.

 

Wei L.C., Shi M., Cao R., Chen L.W. and Chan Y.S., Nestin small interfering RNA (siRNA) reduces cell growth in cultured astrocytoma cells. , Brain Research. 2008, 1196: 103-112.

 

Wong E.Y.M., Chan Y.S., Cheah K.S.E. and Sham M.H., The role of Hox genes in dorsoventral patterning and morphogenesis of inner ear. , Gordon Research Conference on Auditory System in Colby-Sawyer College, New Hampshire, USA. 29th June- 4th July . 2008.

 

Wong E.Y.M., Chan Y.S., Cheah K.S.E. and Sham M.H., The role of Hox genes in otic vesicle patterning in Six1 regulatory pathway. , Gordon Research Conference on Auditory System (Graduate Research Seminar) in Colby-Sawyer College, New Hampshire, USA, 27-28th June. 2008.

 

Yuen Y.L., Kwok J.C.F., Lau J.W.K., Chan Y.S. and Shum D.K.Y., Commissural projection from the vestibular nucleus in rat embryos: role of chondroitin sulfates, Soc. Neuroscience Abstract (U.S.A.): 870.6. 2007.

 

Zhang J., Zhu J.N., Tang B., Li H.Z., Han X.H., Yung W.H., Chan Y.S. and Wang J.J., Histamine selectively excites projection neurons rather interneurons in the cerebellar nuclei. Neuroscience Bulletin 23: 64, 7th Biennial Conference of Chinese Society for Neuroscience. Hangzhou, China. Oct. 24-28. 2007.

 

Zhang Y., Liu J., Chan Y.S. and Shum D.K.Y., Heparanase expression in Schwann cells as revealed in injured sciatic nerves and dorsal root ganglion explant culture, . Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, # POS-SAT-55.

 

Zhang Y., Chau C.H., Lai C.H., Chan Y.S. and Shum D.K.Y., Heparanase upregulation in astrocytes and macrophages recruited to the injured spinal cord. Neuroscience Research 58: S145, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007.

 

Zhang Z., Yu Y.Q., Liu C.H., Chan Y.S. and He J., Frequency tuning and firing pattern properties of auditory thalamic neurons: An in vivo intracellular recording from the guinea pig., Neuroscience. 2008, 151: 293-302.

 

Researcher : Chung SSM

Project Title:

Osmoregulation in lens

Investigator(s):

Chung SSM

Department:

Institute of Molecular Biology

Source(s) of Funding:

Outstanding RGC Projects

Start Date:

09/1998

 

Abstract:

It is imperative to have a better understanding of the mechanisms leading to various cataracts such that preventive measures can be developed. Since maintaining the ionic strength and ionic composition in the lens is essential for the transparency of the lens, we plan to study the mechanism of osmoregulation in this organ. Transgenic mice that over-express aldose reductase and sodium dependent myoinositol transporter in their lenses will be used to increase the lens' level of sorbitol and myoinositol, respectively. We will determine if high level of these osmolytes causes cataract development, and see when the level of one osmolyte is high, will the lens compensate by reducing the level of other osmolytes. We will also determine whether high intracellular osmotic pressure affects the regulation of expression of genes that control the level of osmolytes such as aldose reductase, sodium dependent myoinositol transporter, and taurine transporter.

 

Project Title:

Effects of early postnatal feeding on fatty acid metabolism

Investigator(s):

Chung SSM, Sheng HP

Department:

Institute of Molecular Biology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

11/2002

 

Abstract:

Both epidemiological studies and animal studies have shown that prenatal and early postnatal nutrition not only affects infant growth and development, it also predetermines future metabolism, performance adn morbidity. This study addresses the problems of nutritional environment during the sucking period on lipid metabolism and later-life obesity.

 

Project Title:

Polyol pathway in diabetic dyslipidemia

Investigator(s):

Chung SSM

Department:

Institute of Molecular Biology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

01/2003

 

Abstract:

To determine if AR null mutation reduces plasma TG levels in diabetic mice; to determine if ARI reduces plasma TG in diabetic mice; to determine if AR null mutation or ARI nor ARI normalizes LDL and HDL leviabetic mice.

 

Project Title:

Mechanism of Nogo-A inhibition of axonal regeneration

Investigator(s):

Chung SSM, Chung SK

Department:

Institute of Molecular Biology

Source(s) of Funding:

NSFC/RGC Joint Research Scheme

Start Date:

01/2003

 

Abstract:

To develop Nogo gene knockout mice to determine the role of this gene in the inhibition of axonal regeneration and its physiological functions; to develop transgenic mice that overexpress Nogo-A in their Schwann cells to determine if that would make their PNS non-permissive for axonal regeneraton; to develop transgenic mice that overexpress mutant Nogo-A with the Nogo-66 or aa623-640 region deleted to determine which domain is responsible for the inhibitory effect of Nogo-A.

 

Project Title:

The polyol pathway as a thrifty pathway promoting energy storage

Investigator(s):

Chung SSM

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

09/2003

 

Abstract:

To determine the in vivo conversion of glucose to fatty acids and triglycerides in normal mice and mice deficient in AR; to determine if AR deficiency affects the activities of lipogenic/adipogenic transcriptional factors including ADD1/SREBP-1 and CHOP-C/EBP-[alpha], anti-obesity transcriptional factor FOXC2, and enzymes fatty acid synthase (FAS) and stearoyl CoA desaturase (SCD); to assess whether administration of inhibitors of aldose reductase (ARI) will affect obesity and improve glucose tolerance in the agouti yellow mice; to determine whether the effects of aldose reductase deficiency on agouti-induced lipogenesis and obesity can be duplicated in diet-induced obesity models.

 

Project Title:

Aldose reductase in diabetic cataract

Investigator(s):

Chung SSM

Department:

Institute of Molecular Biology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

10/2004

 

Abstract:

To determine if the antioxidant can prevent AR-induced slow-developing cataract; to determine the osmolyte content and oxidative status of the slow-developing cataract; to compare the morphological changes in the acute diabetic cataract and the slow developing cataract; to determine if there is apoptosis in the epithelial cells of the precataractous lenses.

 

List of Research Outputs

 

Cheung A.K.H., Lo A.C.Y., So K.F., Chung S.S.M. and Chung S.K., Gene deletion and pharmacological inhibition of aldose reductase protect against retinal ischemic injury, Experimental Eye Research. 2007, 85: 608-616.

 

Chung S.S.M., Recent advances in polyol pathway and diabetic complications, Croucher Foundation Advance Study Institute on "Recent advances in diabetes and its complications" Hong Kong. 2007.

 

Lo A.C.Y., Cheung A.K.H., Yeung C.M., He Q., Chiu J., Chung S.S.M. and Chung S.K., Deletion of aldose reductase leads to protection against cerebral ischemic injury., Journal of Cerebral Blood Flow and Metabolism. 2007, 27: 1496-1509.

 

Qiu L., Wu X., Chau J.F.L., Szeto Y.Y., Tam W.Y., Guo Z., Chung S.K., Oates P.J., Chung S.S.M. and Yang J.Y., Aldose Reductase Regulates Hepatic Peroxisome Proliferator-activated Receptor {alpha} Phosphorylation and Activity to Impact Lipid Homeostasis., Journal of Biological Chemistry. 2008, 283: 17175-17183.

 

Tang W.H., Wu S., Wong T.M., Chung S.K. and Chung S.S.M., Polyol Pathway Mediates Iron-induced Oxidative Injury In Ischemic-reperfused Rat Heart, Free Radical Biology Medicine. 2008, 45: 602-10.

 

Xu S., Wong C.L., Tong H.Y., Chung S.S.M. and Ko C.B., Phosphorylation By Casein Kinase 1 Regulates Tonicity-induced Osmotic Reponse Element-binding Protein/tonicity Enhancer-binding Protein Nucleocytoplasmic Trafficking, Journal Of Biological Chemistry. 2008, 283: 17624-34.

 

Researcher : Fung CK

List of Research Outputs

 

Ao Q., Wang A.J., Chen G.Q., Zuo H.C., Zhang X.F., Fung C.K., Tsui Y., Shum D.K.Y. and Chan Y.S., Fabrication and characterization of multi-channel chitosan nerve conduit and its potential application, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 30. March 1-2. 2008.

 

Fung C.K., Shum D.K.Y. and Chan Y.S., Derivation of Schwann cell from mammalian skin for use in axonal degeneration, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 16. March 1-2. 2008.

 

Researcher : Fung ML

Project Title:

Renin-angiotensin system in the carotid body: its expression and functional implications in intermittent hypoxia

Investigator(s):

Fung ML, Tipoe GL, Leung PS

Department:

Physiology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

07/2006

 

Abstract:

I quoted the project objectives and long term impact of the "fundable but not funded" CERG project entitled: "Renin-angiotensin system in the carotid body: its expression and functional implications in intermittent hypoxia " submitted in last RGC exercise:"The long-term objective is to delineate (1) the physiological mechanisms underlying the modulation of carotid chemoreceptor functions and (2) the pathophysiological pathways by which mediate changes in the cardiopulmonary activities during intermittent hypoxia (IH). Chemoreceptors in the carotid body are central to the cardiopulmonary responses to acute hypoxia, and the carotid body plays an important role in the pathogenic events in IH. We have recently shown the expression of functional angiotensin II receptors (AT1) in the chemosensitive cells and a local renin-angiotensin system (RAS) in the carotid body. These data prompt us to propose the following specific aims for examining the general hypothesis that the RAS in the carotid body plays an important role in the modulation of carotid chemoreceptor activity in IH.Specific aim 1 is to determine the effect of IH on the gene expression of RAS in the carotid body. Specific hypothesis 1 is that the expression of RAS is upregulated in the carotid body with IH compared with those of the normoxic control and continuous hypoxia (CH). ..Specific aim 2 is to determine the functional changes associated with the RAS expression in the carotid body in IH. Specific hypothesis 2 is that increased expression of ACE and AT1 receptors in IH carotid body enhances ACE activity, the [Ca++]i response to angiotensin II (AngII) in the glomus cells and the afferent nerve activity in the chemoreceptor. "

 

Project Title:

Renin-angiotensin system in the carotid body: its expression and functional implications in intermittent hypoxia

Investigator(s):

Fung ML, Tipoe GL

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

01/2007

 

Abstract:

To determine the effect of intermittent hypoxia (IH) on the gene expression of RAS in the carotid body; to determine the functional changes associated with the RAS expression in the carotid body in IH; to determine the effect of IH on the expression and function of AT4 receptor in the carotid body.

 

Project Title:

Expressions and roles of hypoxia-inducible factors in the carotid body during intermittent hypoxia

Investigator(s):

Fung ML, Tipoe GL

Department:

Physiology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

07/2007

 

Abstract:

The long term objective is to delineate the pathophysiological pathways by which mediate changes in the cardiopulmonary activities during intermittent hypoxia (IH). Carotid chemoreceptors are central to the cardiopulmonary response to hypoxia, and the carotid body (CB) plays an important role in the pathogenic events in IH. We have recently shown the CB expression of hypoxia-inducible factor-1 (HIF-1) and HIF-target genes and the HIF pathway plays significant roles in the structural and functional changes of the rat CB in continuous hypoxia (CH). This proposal is focused on the IH because many hypoxic episodes are not continuous but rather intermittent in clinical conditions, causing a unique set of cardiovascular morbidity including systemic hypertension, in which the CB plays a significant role. These data prompt us to propose the following specific aim for examining the general hypothesis that the HIF pathway plays an important role in the CB response to IH.Specific aim of the proposal is to determine the effect of IH on the CB expression of HIF subunits and HIF-target genes.Specific hypothesis is that CB expressions of HIF-α subtypes and individual HIF-target genes are increased in IH compared with the CH and normoxic control.The mRNA levels of the genes encoding HIF-α subtypes namely HIF-1α, -2α and -3α and the subunit HIF-1β, and HIF-target genes encoding proteins including vascular endothelial growth factor (VEGF), VEGF receptor subtypes (VEGFR), VEGFR-1 and VEGFR-2, endothelin-1 (ET-1), nitric oxide (NO) synthases, and tyrosine hydroxylase (TH) in the CBs collected from IH rats at different time points of treatment will be compared with those of the CH and normoxic control using real-time PCR and in-situ hybridization methods. Also, the DNA-binding activity of the HIF will be determined by electrophoretic mobility shift assays. The protein levels will be confirmed by Western-blot. Immunohistochemical studies will be performed to localize the proteins in the CB with double-labeling of the immunoreactivity of TH as the cellular marker for the chemosensitive (glomus) cells that are catecholamine-containing. It is speculated that the mRNA and protein levels of the HIF-αsubunits and HIF target genes, but not the HIF-1β, are increased in the IH carotid bodies compared with those of the controls. Thus increases in expressions of individual HIF-α subtypes and HIF activity in IH carotid body can activate the expression of the target genes responsible for the CB functional changes in IH.

 

List of Research Outputs

 

Fung M.L. and Leung P.S., “Frontiers in Research of the Renin-Angiotensin System on Human Disease”, 7th Volume in the book series of Proteases in Biology and Disease, Role of local renin-angiotensin system in the carotid body and in diseases. Springer, 2008, 155-177.

 

Ho C.T., Tipoe G.L., Liong E.C., Leung T.M., Lau T.Y.H., Fung M.L. and Nanji A.A., Decreased adiponectin and antioxidant enzymes are associated with necroinflammatory changes and fibrosis in a rat model for non-alcoholic fatty liver disease (NAFLD), Hepatology International. 2008, 2: S151-S152.

 

Hung M.W., Kravtsov G.M., Lau C.F. and Fung M.L., Melatonin attenuates aortic inflammation, endothelial dysfunction and hypertensive response to chronic intermittent hypoxia in rats, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine,. 2007, 15(2):91: 17.

 

Hung M.W., Tipoe G.L., Poon A.M.S., Reiter R.J. and Fung M.L., Protective effect of melatonin against hippocampal injury of rats with intermittent hypoxia, Journal of Pineal Research. 2008, 44: 214-221.

 

Lam S.S.Y., Tipoe G.L., Liong E.C. and Fung M.L., Chronic hypoxia enhances the expression and function of proinflammatory cytokines in the rat carotid body, 12th Research Postgraduate Symposium, HKU. 2007, 1-12.

 

Lam S.S.Y., Tipoe G.L., Liong E.C. and Fung M.L., Differential expressions and roles of hypoxia-inducible factor-1a, -2a and -3a in the rat carotid body during chronic and intermittent hypoxia, Histology and Histopathology. 2008, 23: 271-280.

 

Lam S.S.Y., Tipoe G.L., Liong E.C. and Fung M.L., Functional upregulation of proinflammatory cytokines and local inflammation in the rat carotid body during chronic hypoxia, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (Journal of the Hong Kong College of Cardiology). 2007, 15(2):91: 18.

 

Lam S.Y.S., Tipoe G.L., Liong E.C. and Fung M.L., Chronic hypoxia upregulates the expression and function of proinflammatory cytokines in the rat carotid body, Histochemistry and Cell Biology. 2008, 130: 549-559.

 

Leung T.M., Tipoe G.L., Liong E.C., Lau T.Y.H., Fung M.L. and Nanji A.A., Nitric oxide inhibitor or donor reverses the induction of hypoxia-inducible factor (HIF-1á) in chronic liver fibrosis in mice, XIth International Congress of Toxicology, Montreal, Canada. 2007.

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Melatonin ameliorates hippocampal nitric oxide production and large-conductance calcium-activated potassium channel activity in chronic intermittent hypoxia, Journal of Pineal Research. 2008, 44: 234-243.

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Nitric oxide deficit in chronic intermittent hypoxia impairs large conductance calcium-activated potassium channel activity in rat hippocampal neurons, Free Radical Biology and Medicine. 2008, 44(4): 547-557.

 

Yeung H.M., Hung M.W. and Fung M.L., Cardioprotective effect of melatonin on impaired calcium homeostasis, myocardial and ischemia-reperfusion injuries in chronically hypoxic rats, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (Journal of the Hong Kong College of Cardiology). 2007, 15(2):88: 10.

 

Yeung H.M., Hung M.W. and Fung M.L., Melatonin ameliorates calcium homeostasis in myocardial and ischemia-reperfusion injury in chronically hypoxic rats, 12th Research Postgraduate Symposium, HKU. 2007, 1-39.

 

Yeung H.M., Hung M.W. and Fung M.L., Melatonin ameliorates impaired calcium homeostasis, myocardial and ischemia-reperfusion injuries by preserving the sarcoplasmic reticulum calcium handling proteins and anti-oxidant enzymes in intermittent hypoxic rats, The Third International Symposium on Healthy Aging, HKU, Hong Kong. 2008, 15.

 

Researcher : Hung MW

List of Research Outputs

 

Hung M.W., Kravtsov G.M., Lau C.F. and Fung M.L., Melatonin attenuates aortic inflammation, endothelial dysfunction and hypertensive response to chronic intermittent hypoxia in rats, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine,. 2007, 15(2):91: 17.

 

Hung M.W., Tipoe G.L., Poon A.M.S., Reiter R.J. and Fung M.L., Protective effect of melatonin against hippocampal injury of rats with intermittent hypoxia, Journal of Pineal Research. 2008, 44: 214-221.

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Melatonin ameliorates hippocampal nitric oxide production and large-conductance calcium-activated potassium channel activity in chronic intermittent hypoxia, Journal of Pineal Research. 2008, 44: 234-243.

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Nitric oxide deficit in chronic intermittent hypoxia impairs large conductance calcium-activated potassium channel activity in rat hippocampal neurons, Free Radical Biology and Medicine. 2008, 44(4): 547-557.

 

Yeung H.M., Hung M.W. and Fung M.L., Cardioprotective effect of melatonin on impaired calcium homeostasis, myocardial and ischemia-reperfusion injuries in chronically hypoxic rats, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (Journal of the Hong Kong College of Cardiology). 2007, 15(2):88: 10.

 

Yeung H.M., Hung M.W. and Fung M.L., Melatonin ameliorates calcium homeostasis in myocardial and ischemia-reperfusion injury in chronically hypoxic rats, 12th Research Postgraduate Symposium, HKU. 2007, 1-39.

 

Yeung H.M., Hung M.W. and Fung M.L., Melatonin ameliorates impaired calcium homeostasis, myocardial and ischemia-reperfusion injuries by preserving the sarcoplasmic reticulum calcium handling proteins and anti-oxidant enzymes in intermittent hypoxic rats, The Third International Symposium on Healthy Aging, HKU, Hong Kong. 2008, 15.

 

Researcher : Hwang ISS

List of Research Outputs

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of Adrenomedullin and Its Receptors in the Reproductive System of the Rat, Effects on Steroid Secretion in Rat Ovary,Biol Reprod. 2008, 79:200.

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: Effects on steroid secretion in rat ovary, Biology of Reproduction. 2008, 79: 200-208.

 

Researcher : Kravtsov GM

List of Research Outputs

 

Hung M.W., Kravtsov G.M., Lau C.F. and Fung M.L., Melatonin attenuates aortic inflammation, endothelial dysfunction and hypertensive response to chronic intermittent hypoxia in rats, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine,. 2007, 15(2):91: 17.

 

Researcher : Lai CH

List of Research Outputs

 

Chan Y.S., Tse Y.C., Lai S.K., Lai C.H. and Yung W.H., Developmental changes in excitatory and inhibitory transmission at central vestibular synapses of rats. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007.

 

Chan Y.S., Lai C.H., Lai S.K., Li C., Ma C.W. and Tse Y.C., The differential role of glutamate receptors in postnatal spatial recognition. Journal of Neurochemistry, 106 Suppl: 6, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, China. June 24-27. 2008.

 

Lai C.H., Yiu C.N.S., Tse Y.C. and Chan Y.S., Functional maturation of vestibular-related brainstem neurons: Postsynaptic currents and responsiveness to horizontal rotational-/translational head movements, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, # POS-SAT-280.

 

Lai C.H., Yiu C.N.S. and Chan Y.S., Postnatal development of canal-related brainstem neurons responsive to three-dimensional rotations in rats, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 23. March 1-2. 2008.

 

Lai C.H., Yiu C.N.S. and Chan Y.S., Postnatal development of canal-related brainstem neurons responsive to vertical head rotation in rats. Neuroscience Bulletin 23: 83, 7th Biennial Conference of Chinese Society for Neuroscience. Hangzhou, China. Oct. 24-28. 2007.

 

Lai S.K., Lai C.H., Wong T.P., Yung W.H. and Chan Y.S., Contribution of glutamate receptors to the maturation of central vestibular neurons in rats, Soc. Neuroscience Abstract (U.S.A.): 180.18. San Diego. 2007.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Differential involvement of perineuronal net components in functional modifications of rat vesticular nucelar neurons, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 3. March 1-2. 2008.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Modifications in perineuronal nets of rat vestibular nuclear neurons during postnatal development and in injury, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12 . 2007, 58: S98.

 

Ng K.P., Lai C.H., Tse Y.C. and Chan Y.S., Thalamic neurons detect both rotational and translational motions in the horizontal plane. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007, 58.

 

Ng K.P., Lau P.Y.P., Lai C.H. and Chan Y.S., Topographic distribution of thalamic neurons responsive to angular and linear head movements. , Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 26. March 1-2. 2008.

 

Tse Y.C., Lai C.H., Lai S.K., Liu J.X., Yung K.K.L., Shum D.K.Y. and Chan Y.S., Developmental expression of NMDA and AMPA receptor subunits in vestibular nuclear neurons that encode gravity-related horizontal orientations. , Journal of Comparative Neurology. 2008, 508: 343-364.

 

Zhang Y., Chau C.H., Lai C.H., Chan Y.S. and Shum D.K.Y., Heparanase upregulation in astrocytes and macrophages recruited to the injured spinal cord. Neuroscience Research 58: S145, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007.

 

Researcher : Lai SK

List of Research Outputs

 

Chan Y.S., Tse Y.C., Lai S.K., Lai C.H. and Yung W.H., Developmental changes in excitatory and inhibitory transmission at central vestibular synapses of rats. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007.

 

Chan Y.S., Lai C.H., Lai S.K., Li C., Ma C.W. and Tse Y.C., The differential role of glutamate receptors in postnatal spatial recognition. Journal of Neurochemistry, 106 Suppl: 6, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, China. June 24-27. 2008.

 

Lai S.K., Lai C.H., Wong T.P., Yung W.H. and Chan Y.S., Contribution of glutamate receptors to the maturation of central vestibular neurons in rats, Soc. Neuroscience Abstract (U.S.A.): 180.18. San Diego. 2007.

 

Lai S.K., Wong T.P., Yung W.H., Shum D.K.Y. and Chan Y.S., Maturation profile of ionotropic glutamate receptors in central vestibular neurons of rats. Journal of Neurochemistry 106 Suppl: 43-44. , 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, June 24-26. 2008.

 

Lai S.K., Wong T.P., Yung W.H. and Chan Y.S., Postnatal development of ionotropic glutamate receptors in central vestibular neurons of rats. , Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 24. March 1-2. 2008.

 

Lai S.K., Wong T.P., Yung W.H. and Chan Y.S., Postnatal modification in glutamatergic synapses within the rat vestibular nucleus, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, #POS-SAT-281.

 

Tse Y.C., Lai C.H., Lai S.K., Liu J.X., Yung K.K.L., Shum D.K.Y. and Chan Y.S., Developmental expression of NMDA and AMPA receptor subunits in vestibular nuclear neurons that encode gravity-related horizontal orientations. , Journal of Comparative Neurology. 2008, 508: 343-364.

 

Researcher : Lam SSY

List of Research Outputs

 

Lam S.S.Y., Tipoe G.L., Liong E.C. and Fung M.L., Chronic hypoxia enhances the expression and function of proinflammatory cytokines in the rat carotid body, 12th Research Postgraduate Symposium, HKU. 2007, 1-12.

 

Lam S.S.Y., Tipoe G.L., Liong E.C. and Fung M.L., Differential expressions and roles of hypoxia-inducible factor-1a, -2a and -3a in the rat carotid body during chronic and intermittent hypoxia, Histology and Histopathology. 2008, 23: 271-280.

 

Lam S.S.Y., Tipoe G.L., Liong E.C. and Fung M.L., Functional upregulation of proinflammatory cytokines and local inflammation in the rat carotid body during chronic hypoxia, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (Journal of the Hong Kong College of Cardiology). 2007, 15(2):91: 18.

 

Researcher : Lam SYS

List of Research Outputs

 

Lam S.Y.S., Tipoe G.L., Liong E.C. and Fung M.L., Chronic hypoxia upregulates the expression and function of proinflammatory cytokines in the rat carotid body, Histochemistry and Cell Biology. 2008, 130: 549-559.

 

Researcher : Lau CF

List of Research Outputs

 

Hung M.W., Kravtsov G.M., Lau C.F. and Fung M.L., Melatonin attenuates aortic inflammation, endothelial dysfunction and hypertensive response to chronic intermittent hypoxia in rats, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine,. 2007, 15(2):91: 17.

 

Researcher : Lau JWK

List of Research Outputs

 

Yuen Y.L., Kwok J.C.F., Lau J.W.K., Chan Y.S. and Shum D.K.Y., Commissural projection from the vestibular nucleus in rat embryos: role of chondroitin sulfates, Soc. Neuroscience Abstract (U.S.A.): 870.6. 2007.

 

Researcher : Lau PYP

List of Research Outputs

 

Ng K.P., Lau P.Y.P., Lai C.H. and Chan Y.S., Topographic distribution of thalamic neurons responsive to angular and linear head movements. , Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 26. March 1-2. 2008.

 

Researcher : Lee HC

List of Research Outputs

 

Liu Q., Kriksunov I. .A., Moreau C., Graeff R., Potter B. .V. .L., Lee H.C. and Hao Q., Catalysis Associated Conformational Changes Revealed By Human Cd38 Complexed With A Non-hydrolysable Substrate Analog. , Journal Of Biological Chemistry. 2007, 282: 24825-24832.

 

Tao R., Lau C.P., Lee H.C. and Li G.R., Cyclic ADP-ribose increases human mesenchymal stem cell proliferation through the modulation of spontaneous Ca2+ oscillations. , FEBS J / 33rd FEBS Congress and 11th IUBMB Conference, Athens, Greece. . 2008, 275(suppl 1): 295.

 

Researcher : Li C

List of Research Outputs

 

Chan Y.S., Lai C.H., Lai S.K., Li C., Ma C.W. and Tse Y.C., The differential role of glutamate receptors in postnatal spatial recognition. Journal of Neurochemistry, 106 Suppl: 6, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, China. June 24-27. 2008.

 

Researcher : Li GR

Project Title:

Volume-sensitive chloride current and cell volume regulation in human atrial myocytes

Investigator(s):

Li GR, Lau CP, Chiu SW, Tse HF

Department:

Medicine

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

12/2001

 

Abstract:

To determine the intracellular signaling pathways that regulate ICl.vol and cell volume in human atrium; to determine whether ICl.vol is persistently activated in atrial myocytes from patients with dilated atrial cardiomyopathy.

 

Project Title:

Studies on Ion Channels in Human Pre-adipocytes

Investigator(s):

Li GR, Lau CP

Department:

Medical Faculty

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

05/2006

 

Abstract:

Ion channels play an important role in the physiological activities of many types of cells. In excitable cells, activation of ion channels generates action potentials and participates in excitation-contraction (muscles), excitation-secretion (glands), and impulse conduction (nerve and muscles), as well as repolarizing cell membrane potential. In proliferative cells, ion channels are found to be required for cell proliferation. Recent studies have demonstrated that several K channels, eg. inward rectifier K channels (IKir), delayed rectifier K channels (IKDR), ATP-sensitive K channel (IKATP), are involved in the cell cycling of lymphocytes, neuronal glial cells (e.g. astrocytes), epithelial cells, and cancer cells. Blockade of IKDR is believed to inhibit cell proliferation, whereas inhibition of IKIR promotes proliferation in rat spinal cord astrocytes. In addition, volume-sensitive chloride current (ICl.vol) is involved in the proliferation of several types of cells including vascular smooth muscle and endothelial cells, cancer cells etc. These studies indicate an important relationship between ion channels and cell proliferation. There has been a dramatic increase in the incidence of obesity resulting from an excess of white adipose tissue. Obesity is a prevalent health hazard in industrial countries, and is closely associated with the occurrence of type 2 diabetes and cardiovascular disease. Preadipocytes are the origin of fat cells of white adipose tissue. For the past two decades, in vitro systems have been extensively used to study adipocyte differentiation with preadipocytes. Although significant progress has been made in the dissection of the molecular and cellular events taking place during the transition from undifferentiated preadipocytes into mature round fat cells, control of adipocyte differentiation is not completely understood, especially ion channels and thire physiological roles in proliferation/differentiation have not been studied. The present proposal was to study ion channel expression and thire molecular identidies in human pre-adipocytes commercially obtained from CellSicence using whole-cell patch clamp and RT-PCR techniques, and to provide the experimental data for obtaining external RGC grant to further study whether/how ion channels are involved in proliferation/differentiation in human pre-adipocytes.

 

Project Title:

Studies on ion channels and cell proliferation in human cardiac fibroblasts

Investigator(s):

Li GR, Lau CP

Department:

Medical Faculty

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

01/2007

 

Abstract:

To analyze the molecular identities of IKCa, IKDR, ICI.vol, and INa, in human cardiac fibroblasts with RT-PCR, Western blot analysis, and patch clamp technique; to test the hypothesis that these four types of channels (IKCa, IKDR, ICI.vol, and INa are involved in the proliferation of human cardiac fibroblasts. Cell proliferation assays will be conducted in the absence and presence of channel blockers, short interfering RNA (siRNA) oligos targeted to IKDR, IKCa, ICI.vol, and INa and channels, and/or signal modulators.

 

 

Researcher : Li L

List of Research Outputs

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of Adrenomedullin and Its Receptors in the Reproductive System of the Rat, Effects on Steroid Secretion in Rat Ovary,Biol Reprod. 2008, 79:200.

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: Effects on steroid secretion in rat ovary, Biology of Reproduction. 2008, 79: 200-208.

 

Researcher : Li M

List of Research Outputs

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Melatonin ameliorates hippocampal nitric oxide production and large-conductance calcium-activated potassium channel activity in chronic intermittent hypoxia, Journal of Pineal Research. 2008, 44: 234-243.

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Nitric oxide deficit in chronic intermittent hypoxia impairs large conductance calcium-activated potassium channel activity in rat hippocampal neurons, Free Radical Biology and Medicine. 2008, 44(4): 547-557.

 

Researcher : Li YY

List of Research Outputs

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of Adrenomedullin and Its Receptors in the Reproductive System of the Rat, Effects on Steroid Secretion in Rat Ovary,Biol Reprod. 2008, 79:200.

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: Effects on steroid secretion in rat ovary, Biology of Reproduction. 2008, 79: 200-208.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat., The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2007, 62: 1346-1351.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat, J Gerontol A Biol Sci Med Sci. 2007, 62: 1346-51.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat, Journal of Gerontology: Biological Sciences. 2007, 62A(12): 1346-1351.

 

Researcher : Li YY

List of Research Outputs

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of Adrenomedullin and Its Receptors in the Reproductive System of the Rat, Effects on Steroid Secretion in Rat Ovary,Biol Reprod. 2008, 79:200.

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: Effects on steroid secretion in rat ovary, Biology of Reproduction. 2008, 79: 200-208.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat., The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2007, 62: 1346-1351.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat, J Gerontol A Biol Sci Med Sci. 2007, 62: 1346-51.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat, Journal of Gerontology: Biological Sciences. 2007, 62A(12): 1346-1351.

 

Researcher : Lung MAKY

Project Title:

Endothelins in nasal mucosa: role in nasal congestion and decongestion

Investigator(s):

Lung MAKY

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2004

 

Abstract:

To investigate the role of endothelins on the nasal mucosa, eliciting nasal congestion or decongestion, as well as the underlying vascular events and control mechanisms.

 

Project Title:

Muscarinic receptor subtypes in the control of nasal patency

Investigator(s):

Lung MAKY

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2006

 

Abstract:

Rhinitis, affecting about 20% of the world population and with increasing prevalence worlwide, is characterized by symptoms of mucosal hypereamia, engorgement, rhinorrea and nasal obstruction. In vivo studies in dogs show that acetylcholine induces nasal congestion at low doses (Lung & Wang, Ann Otol Rhinol Laryngol 1994; 103:646-651). Recent in vitro studies confirm that acetylcholine at low concentrations relaxes posterior collecting veins but contracts other collecting and outflow veins (Wang & Lung, Eur Resp J published online on May 31, 2006 as 10.1183/09031936.06.00077105). According to the vascular arrangements of the nasal mucosa, activation of muscarinic receptors in the posterior collecting veins will increase vascular capacitiance while activation of muscarinic receptors in the outflow veins will impair venous drainage. Both mechanisms acting together can easily promote nasal congestion especially when the number and reactivity of the mucarinic receptors are increased. An increase in the number of muscarinic receptors has been found in the nasal mucosa of human or animal models with nasal allergy (Naminatsu et al, Jpn J Pharmacol 1992; 59: 427-437). Radiolabelled ligand binding, autoradiography, competitive binding analysis and imumological studies suggest that M1, M2 and M3 receptor subtypes are present in the sinusoidal venous vessels of human inferior turbinate mucosa and among them M3 receptors are the most extensively distributed (Nakaya et al., Ann Otol Rhinol Laryngol 2002; 111: 593-597). However, the presence and distribution of muscarinci receptor subtypes in the collecting and outflow veins of the nasal mucosa are still unknown.The objective of this project is to to investigate the functional role of muscarinic receptors in the control of nasal patency. To achieve this, we will1. perform in vivo functional studies to characterize the muscarinic receptor subtypes present on the resistance and and capacitance vessels and to define their individual role in the control of nasal vascular and airway resistances.2. perform in vitro studies to characterize the muscarinic receptors present on the nasal collecting and outflow veins and to define their individual role in the control of airway resistance.3. perform immunohistochemical studies on the localization of muscarinic subtype receptors (M1, M2 and M3) as to confirm their presence and distribution in the nasal vasculature.

 

 

Researcher : Lung MKY

Project Title:

Endothelins in nasal mucosa: role in nasal congestion and decongestion

Investigator(s):

Lung MAKY

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2004

 

Abstract:

To investigate the role of endothelins on the nasal mucosa, eliciting nasal congestion or decongestion, as well as the underlying vascular events and control mechanisms.

 

Project Title:

Muscarinic receptor subtypes in the control of nasal patency

Investigator(s):

Lung MAKY

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2006

 

Abstract:

Rhinitis, affecting about 20% of the world population and with increasing prevalence worlwide, is characterized by symptoms of mucosal hypereamia, engorgement, rhinorrea and nasal obstruction. In vivo studies in dogs show that acetylcholine induces nasal congestion at low doses (Lung & Wang, Ann Otol Rhinol Laryngol 1994; 103:646-651). Recent in vitro studies confirm that acetylcholine at low concentrations relaxes posterior collecting veins but contracts other collecting and outflow veins (Wang & Lung, Eur Resp J published online on May 31, 2006 as 10.1183/09031936.06.00077105). According to the vascular arrangements of the nasal mucosa, activation of muscarinic receptors in the posterior collecting veins will increase vascular capacitiance while activation of muscarinic receptors in the outflow veins will impair venous drainage. Both mechanisms acting together can easily promote nasal congestion especially when the number and reactivity of the mucarinic receptors are increased. An increase in the number of muscarinic receptors has been found in the nasal mucosa of human or animal models with nasal allergy (Naminatsu et al, Jpn J Pharmacol 1992; 59: 427-437). Radiolabelled ligand binding, autoradiography, competitive binding analysis and imumological studies suggest that M1, M2 and M3 receptor subtypes are present in the sinusoidal venous vessels of human inferior turbinate mucosa and among them M3 receptors are the most extensively distributed (Nakaya et al., Ann Otol Rhinol Laryngol 2002; 111: 593-597). However, the presence and distribution of muscarinci receptor subtypes in the collecting and outflow veins of the nasal mucosa are still unknown.The objective of this project is to to investigate the functional role of muscarinic receptors in the control of nasal patency. To achieve this, we will1. perform in vivo functional studies to characterize the muscarinic receptor subtypes present on the resistance and and capacitance vessels and to define their individual role in the control of nasal vascular and airway resistances.2. perform in vitro studies to characterize the muscarinic receptors present on the nasal collecting and outflow veins and to define their individual role in the control of airway resistance.3. perform immunohistochemical studies on the localization of muscarinic subtype receptors (M1, M2 and M3) as to confirm their presence and distribution in the nasal vasculature.

 

 

Researcher : Ma CW

List of Research Outputs

 

Chan Y.S., Lai C.H., Lai S.K., Li C., Ma C.W. and Tse Y.C., The differential role of glutamate receptors in postnatal spatial recognition. Journal of Neurochemistry, 106 Suppl: 6, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, China. June 24-27. 2008.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Differential involvement of perineuronal net components in functional modifications of rat vesticular nucelar neurons, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 3. March 1-2. 2008.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Modifications in perineuronal nets of rat vestibular nuclear neurons during postnatal development and in injury, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12 . 2007, 58: S98.

 

Researcher : Ma CW

List of Research Outputs

 

Chan Y.S., Lai C.H., Lai S.K., Li C., Ma C.W. and Tse Y.C., The differential role of glutamate receptors in postnatal spatial recognition. Journal of Neurochemistry, 106 Suppl: 6, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, China. June 24-27. 2008.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Differential involvement of perineuronal net components in functional modifications of rat vesticular nucelar neurons, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 3. March 1-2. 2008.

 

Ma C.W., Lai C.H., Shum D.K.Y. and Chan Y.S., Modifications in perineuronal nets of rat vestibular nuclear neurons during postnatal development and in injury, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12 . 2007, 58: S98.

 

Researcher : Ng KP

List of Research Outputs

 

Ng K.P., Lai C.H., Tse Y.C. and Chan Y.S., Thalamic neurons detect both rotational and translational motions in the horizontal plane. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007, 58.

 

Ng K.P., Lau P.Y.P., Lai C.H. and Chan Y.S., Topographic distribution of thalamic neurons responsive to angular and linear head movements. , Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 26. March 1-2. 2008.

 

Researcher : Ng KP

List of Research Outputs

 

Ng K.P., Lai C.H., Tse Y.C. and Chan Y.S., Thalamic neurons detect both rotational and translational motions in the horizontal plane. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007, 58.

 

Ng K.P., Lau P.Y.P., Lai C.H. and Chan Y.S., Topographic distribution of thalamic neurons responsive to angular and linear head movements. , Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 26. March 1-2. 2008.

 

Researcher : Poon AMS

Project Title:

Adrenomedullin in reproduction: gene expression of adrenomedullin in the female tract and the possible roles of adrenomedullin in the seminal plasma.

Investigator(s):

Poon AMS, O WS, Tang F

Department:

Physiology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

03/2007

 

Abstract:

BACKGROUND Our laboratories have shown that adrenomedullin (AM) is produced by the testis and the male sex accessory glands in the rat including the prostate, seminal vesicle and the epipidymis It inhibits steroidogenesis (Li et al. 2005) and inhibin secretion (Chan et al. unpublished results) and increases secretion in the epididymis (Hwang et al. 2003). AM levels are high in the seminal plasma (Marinoni et al. 2005) and one possible function of AM may be to increase motility (Yeung et al. personal communication). The actions of AM derived from the seminal plasma on the female tract have not been studied. One possible action is to increase cytokine production as vascular endothelial growth factor (VEGF), which is also present in the seminal plasma, has been shown to stimulate cytokine production in cultured human cervical cells (Robertson & Sharkey 2006) and AM is known to stimulate cytokine production in a variety of tissues The increase in cytokine production will in turn stimulate AM production from the uterine cells to form a positive feedback loop. The cytokines would then have inflammatory actions to supplement the anti-microbial actions of AM (Allaker et al. 2006). AM may also decrease the uterine contraction for implantation of the zygote. An inhibitory action of AM on uterine contraction has been reported (Yanagita et al. 2000). Another possible action of AM may be to decrease the production of reactive oxygen species (ROS) which may be important for the survival of the sperm and successful development of the fertilized ovum. It has been reported that the removal of the male sex accessory glands increases the vulnerability of the sperms to oxidative damage by ROS from leucocytes and dead sperms and from the uterine endometrium (O et al. 2006). AM has the ability to reduce the formation of ROS (Yoshimoto et al. 2004) and therefore AM in the seminal plasma may play an important anti-oxidative role in addition to the antioxidant enzymes known to be present in the seminal plasma. Our laboratories have also done some work to show that AM is produced in the ovary and the uterus. However, no work has been done on the rat oviduct. It has been reported that the presence of sperms may induce the production of AM from the mouse oviduct (Fazeli et al. 2004).The possible roles of AM secreted by the oviduct is unknown. It may increase oviductal secretion and/or decrease the motility of the oviduct, which may aid the fertilization of the ovum by the sperms. This possibility needs to be studied. Finally, the effects of AM on implantation and embryo development in early pregnancy have not been studied. Another important aspect is the possible role of AM in reducing immunity during pregnancy. The decrease in immunity during pregnancy is a well-known phenomenon and is important to prevent rejection of the foetus. The ability of AM to reduce immunosurveillance is an important factor for the development of tumours that are stimulated by AM (Zudaire et al. 2003). HYPOTHESIS Adrenomedullin in the seminal fluid may be important in reproduction by increasing the production of cytokines and by reducing the reactive oxygen species in the female tract. Adrenomedullin produced by the female tract may be important for fertilization and later for the maintenance of pregnancy. OBJECTIVES (1) To study the gene expression of AM and its receptor component proteins in the oviduct and the uterus. (Already done) (2) To study the effects of AM on cytokine production and the effect of cytokines on AM production in cultures of uterine fragments. (3) To study the effects of AM on ROS production in cultures of oviduct and uterus. (4) To study the effect of AM on oviductal secretion and motility. (5) To study the effect of AM on implantation and embryo development during early pregnancy. REFERENCES Allaker RP, Grosvenor PW, McAnerney DC, Sheehan BE, Srikanta BH, Pell K and Kapas S. (2006) Peptides 27, 661-666. Faseli A, Affara NA, Hubank M and Holt WV (2004) Biol. Reprod. 71, 60-65. Hwang ISS, Autelitano DJ, Wong PYD, Leung GPH and Tang F (2003) Biol. Reprod. 68, 2005-2012. Li YY, Hwang ISS, O WS and Tang F (2006) Biol. Reprod. 75, 183-188. Marinoni E, Di Iorio R, Villaccio B, Vellucci O, Di Netta T, Sessa M, Letizia C and Cosmi EV (2005) Eur. J. Obstet. Gynecol. Reprod. Biol. 122, 195-198. O WS, Chen H and Chow PH (2006) Mol. Cell. Endocrinol. 250, 80-83. Robertson S and Sharkey DJ (2006) Abstract O18, Society for Reproduction and Fertility Conference 2006, Leeds, UK. Yanagita T, Yamamoto R, Sugano T, Kobayashi H, Uezono Y, Yokoo H, Shiraishi S, Minami SI and Wada A (2000) Br. J. Pharmacol. 130, 1727-1730. Yoshimoto T, Fukai N, Sato R, Sugiyama T, Ozawa N, Schichire\i M and Hirata Y (2004) Endocrinology 145, 3331-3337. Zudaire E, Martiniez A and Cuttitta F (2003) Regul. Pept. 112, 175-183.

 

List of Research Outputs

 

Hung M.W., Tipoe G.L., Poon A.M.S., Reiter R.J. and Fung M.L., Protective effect of melatonin against hippocampal injury of rats with intermittent hypoxia, Journal of Pineal Research. 2008, 44: 214-221.

 

Researcher : Shiu SYW

Project Title:

sPDZD2-induced protein profile changes in prostate cancer

Investigator(s):

Shiu SYW, Yao KM

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2004

 

Abstract:

To express and purify sPDZD2 in E. coli, and to study the effects of the recombinant protein on the protein profiles of human prostate cancer to cells using a 'proteomics' approach.

 

Project Title:

Overexpression of sPDZD2 in prostate cancer cells

Investigator(s):

Shiu SYW, Yao KM

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

10/2005

 

Abstract:

PDZ (PSD95, Discs-large, and ZO-1) domain containing protein 3 (PDZK3), also known as PDZ domain containing protein 2 (PDZD2), is a ubiquitously expressed protein of unknown function which has six PDZ domains. The protein is cleaved in a caspase-dependent mechanism to generate a secreted protein (sPDZD2), which may participate in extracellular signaling [Yeung et al. 2003, EMBO Rep 4: 412]. Interestingly, gene expression of PDZK3 has been reported to be upregulated in prostate cancer when compared with benign tissues Thus, it is believed that PDZK3, also named as activated in prostate cancer protein (AIPC), may play an important role in prostate tumorigenesis [Chaib et al. 2001, Cancer Res 61:2390]. Given that our ongoing studies have demonstrated that sPDZD2 protein is produced and secreted by human prostate cancer cell lines, we would like to postulate that sPDZD2 protein, a cleavage product of PDZD2, may play an important role in prostate cancer pathogenesis by modulating prostate cancer cell growth and differentiation. The objectives of this proposal are to 1) over-express sPDZD2 protein in prostate cancer cells, and 2) to study any effect of the protein on the expression of cell cycle and apoptosis-related proteins in the transfected cancer cells.

 

Project Title:

Structure-function studies of sPDZD2 protein and identification of its cellular targets

Investigator(s):

Shiu SYW, Yao KM

Department:

Physiology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

01/2007

 

Abstract:

Globally, prostate cancer has become the third most common cancer in men, with half a million new cases each year, amounting to about 10% of all male cancers. It is the most common cancer after skin cancer, and is the second leading cause of cancer-related death in men in the United States. The emergence of prostate cancer as a public health problem in developed countries has put tremendous pressure on the healthcare system to provide new and effective treatments. While there is significant research progress on understanding the signaling mechanisms of androgen, a key growth-promoting hormone, in prostate cancer initiation and progression, advanced prostate cancer patients still suffer from inevitable relapse of the disease after receiving the recommended treatment of androgen deprivation therapy (ADT). Clearly, there is an unmet clinical need to develop other novel therapeutic agents which can act effectively alone and/ or in combination with ADT to halt or reverse the progression of advanced prostate cancer. Such demand has fueled the search for novel endo-/para-/auto-crine growth-promoting and growth-inhibitory signaling pathways important in prostate cancer pathogenesis, which may yield new therapeutic agents or targets for anti-prostate cancer drug discovery. Recently, our group has identified the human sPDZD2 protein as a novel autocrine prostate tumor suppressor [Tam et al. Endocrinology 2006;147:5023-5033]. In this project we propose to construct a genetically-modified form of the endogenous human sPDZD2 protein with prostate cancer growth-inhibitory actions on both hormone-sensitive and hormone-insensitive prostate cancer cells. To further maximize the therapeutic potential of this endogenous sPDZD2 protein and to fuel the anti-prostate cancer drug development pipeline by the target-driven approach, efforts would be expended towards the molecular identification of the cellular targets, including the receptors, acted upon by the native and genetically-modified forms of human sPDZD2 protein.

 

List of Research Outputs

 

Shiu S.Y.W., Towards rational and evidence-based use of melatonin in prostate cancer prevention and treatment, Journal of Pineal Research. 2007, 43: 1-9.

 

Tam C.W., Liu V.W.S., Leung W.Y., Yao K.M. and Shiu S.Y.W., The autocrine sPDZD2 protein is a potential p53 activator, AACR Centennial Conference on Translational Cancer Medicine: Technologies to Treatment. 2007, B40.

 

Researcher : Tam CW

List of Research Outputs

 

Tam C.W., Liu V.W.S., Leung W.Y., Yao K.M. and Shiu S.Y.W., The autocrine sPDZD2 protein is a potential p53 activator, AACR Centennial Conference on Translational Cancer Medicine: Technologies to Treatment. 2007, B40.

 

Researcher : Tam KW

List of Research Outputs

 

Tam K.W., Li R.A., Chan Y.S. and Shum D.K.Y., Cloning, recombinant expression and digestion product characterization of chondroitinse ABC I and II, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, #POS-SUN-050.

 

Tam K.W., Li R.A., Chan Y.S. and Shum D.K.Y., Effects of oligosaccharide products on activity of chondroitin sulphate ABC lyase I – Towards improving the efficacy of the enzyme for in vivo treatment, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 4. March 1-2. 2008.

 

Researcher : Tang F

Project Title:

Adrenomedullin in the rat testis: paracrine actions and its interaction with endothelin

Investigator(s):

Tang F, O WS

Department:

Physiology

Source(s) of Funding:

Competitive Earmarked Research Grants (CERG)

Start Date:

09/2004

 

Abstract:

To study the gene expression and levels of adrenomedullin and also its receptors and binding in the whole testis and in the Leydig cells and Sertoli cells, and the types of receptor activity modifying proteins expressed in these cells which will determine whether these cells respond to adrenomedullin or CGRP or both; to investigate the effects of adrenomedullin on steoidogenesis in the Leydig cells, contraction of seminferous tubules and Sertoli cell secretion in the presence or absense of CGRP or endothelin, gonadotrophins and testosterone.

 

Project Title:

Adrenomedullin as a new metabolic hormone: its distribution and actions in the liver, muscles and adipose tissues, and possible roles in metabolic syndrome and in ageing.

Investigator(s):

Tang F

Department:

Physiology

Source(s) of Funding:

Seed Funding Programme for Basic Research

Start Date:

01/2006

 

Abstract:

Overall objective. To study the effects of adrenomedullin (AM) on glucose and fat metabolism in the liver, muscle and fat and its roles in metabolic syndrome. Specific objectives. (1) To study the effects of AM on glucose uptake in the liver, muscles and fat, gluconeogenesis in the liver, lipogenesis and lipolysis in fat, and free fatty acid oxidation in the fat and muscle strips. (2) To study the effects of AM on plasma glucose and FFA levels in vivo. (3) To determine the changes of AM levels and gene expression in animal models of metabolic syndrome: (a) the Zucker rat with deficiency of leptin receptors, and (b) rats fed with a high fat diet. (4) To study the parameters listed in (3) in ageing rats if time and resource permit. Ageing is one specific type of metabolic syndrome in the rat because all old rats are obese as a result of the lack of exercise due to confinement to a small space. Background & Rationale. Adrenomedullin (AM) is a newly discovered peptide hormone, which is widely distributed and have many functions, mostly paracrine. AM and its receptors have been reported in the liver and white adipose tissues and this has been confirmed by us. Our laboratory is the first (and the only one so far) to confirm the presence of AM and its gene expression in the skeletal muscles. The metabolic actions of adrenomedullin is largely unknown. Two other members of the calcitonin family (to which AM belongs) i.e. amylin and CGRP, are known to decrease glucose uptake (Abatty & Cooper 2004) and to increase insulin resistance (Molina et al. 1990). AM, like amylin, is produced by the pancreatic islets and inhibits insulin secretion (Martinez et al. 2006). Our preliminary data suggest that AM may increase insulin-mediated glucose uptake by the rat liver cells and by a mouse adipocyte cell line (3T3L1). Another group (Shimosawa et al. 2005) has reported a possible effect of AM on glucose transport in the soleus msucle of a transgenic mouse. The effects of AM on fat metabolism is controversial. AM may increase lipogenesis (Fukai et al. 2005) but is known to inhibit (Hamancey et al. 2005) or stimualte (Linshield et al. 2005) lipolysis in vitro. We hypothesize that AM may be another adipokine like leptin and adiponectin to decrease insulin resistance but is different from these two in acting mainly locally (paracrine action). It may do so in a number of ways, including increase in glucose uptake, decrease in gluconeogenesis and increase in free fatty acid oxidation. If this hypothessis is correct, one would expect changes in AM level and gene expression in the liver, fat and muscles in metabolic syndrome, such as in the Zucker rats or in obesity induced by a high fed diet. Morevover, there is an increase of oxidative stress in metabolic syndrome (Shimosawa & Fujita 2005) and AM is an important anti-oxidant. Metabolic syndrome is characterized by increases in insulin resistance, obesity, hypertension and oxidative stress (Morley 2004). Hypothesis. The hypothesis is that AM may have important actions in the metabolism of the liver, muscle and adipose tissues to modulate insulin resistance, and hence may play important roles in the development of metabolic syndrome . Reference. 1. Abatty T & Cooper GJ (2004) Peptides 25: 2119-2125. 2. Molina JM, Cooper GJ, Leighton B et al. (1990) Diabetes 39: 260-265. 3. Martinez A, Weaver C, Lopez J et al. (1996) Endocrinology 137: 2626-2632. 4. Shimosawa T & Fujita T (2005) Drug News Perspect. 18: 185-189. 5. Fukai N, Yoshimoto T, Sugiyama T et al. (2005) Amer. J. Physiol. 288: E56-62. 6. Hamacey R, Senard JM, Pathak A et al. (2005) FASEB J. 19: 1045-1047. 7. Linshield P, Saboek D, Zulewski H et al. (2005) Endocrinolgoy 46: 2699-2708. 8. Morley LE (2004) J. Gerontol. 59A: 139-142.

 

Project Title:

Ageing and drenomedullin in the female reproductive system

Investigator(s):

Tang F, O WS

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

10/2006

 

Abstract:

Objectives: (1) To study the age-related changes in adrenomedullin (AM) and the gene expresion of preproAM, its receptor (CRLR, calcitonin receptor-like receptor) and RAMPS (receptor activity modifying proteins) in the ovary, oviduct and testis. (2) To determine the chgnes in AM receptor binding with age in the ovary, oviduct and the uterus by Scatchard plot analysis. (3) To correlate the above with the age-related changes in plasma AM, and serum oestadiol and progesterone levels. Background: In our laboratory, adrenomedullin (AM) , and the gene expression of AM and its receptor components (i.e. CRLR, calcitonin receptor-like receptor, and RAMPs, receptor activity modifying proteins) are found in the ovary, oviduct and utersus of the rat (supportd by an earlier grant by CRCG). In the male, ageing is associated with an increase in the above parmeters in the testis, but a decrease in the sex accessory glands including the seminal vesicles and the ventral prostate (Tang et al. 2006). These changes are believed to be the cause or the result of a decrease in testosterone production by the testis. The changes of AM gene expression in the uterus is related to the oestrogen level (Cameron et al. 2002 ). AM is believed to lay some roles in the female including the development of the corpus luteum (Abe et al. 2000), steroidogenesis (Moriyama et al. 2000), uterine blood flow (Makino et al. 1999), uterine contraction (Upton et al. 1997), implantation (Zhang et al.2005), and the development of the placenta (Penchalani et al. 2004). It is well-established that plasma oestrogen levels decrease with age, particularly after menopause. Hypothesis: We hypothesize that there are age-related changes in AM and its receptor component proteins that may be related to the change in oestrogen levels. As AM is known to increase steroidogenesis, we might expect to find a decrease in ovarian AM with ageing. Similarly, since AM in the uterus is increased by oestorogen, the age-related decrease in plasma oestrogen level would result in a decline in AM in the uterus. These changes in the AM system would impair the proper functioning of the ovary and uterine receptivity. Abe K, Minegishi T, Ibuki Y et al. (2000) Fetil Steril. 74: 141-5 Cameron VA, Auteltintano DJ, Evans JJ et al. (2002) Am. J. Physiol. 282: E139-146. Makino et al. (1999) Eur. J. Pharmacol. 385: 129. Moriyama T, Otani T, Maruo T. Eur. J. Endocrinol. 142: 671-6. Penchalini J, Wimalawansa SJ, Yallampalli C. (2004) Biol. Reprod. 71: 1475-83. Tang F, Li YY and O WS (2006) Abstrtact, Conference of the Society for Reproduction and Fertility. Upton PD, Austin C, Taylor GM et al. (1997) Endocrinology 138: 2506-14. Zhang X, Green KE, Yallampalli C et al. (2005) Biol. Reprod. 73: 619-626.

 

List of Research Outputs

 

Chan Y.F., Tang F. and O W.S., Adrenomedullin in the Rat Testis. II: Its Production, Actions on Inhibin Secretion, Regulation by Follicle-Stimulating Hormone, and Its Interaction with Endothelin 1 in the, Sertoli Cell Biol Reprod . 2008, 78: 780-5.

 

Chan Y.F., O W.S. and Tang F., Adrenomedullin in the rat testis. I: Its production, actions on testosterone secretion, regulation by human chorionic conadotropin, and its interaction with endothelin 1 in the Leydig cell, Biology of Reproduction. 2008, 78: 773-779.

 

Chan Y.F., Tang F. and O W.S., Adrenomedullin in the rat testis. II: Its production, actions on inhibin secretion, regulation by follicle-stimulating hormone, and its interaction with endothelin 1 in the sertoli cell, Biology of Reproduction. 2008, 78: 780-785.

 

Chan Y.F., O W.S. and Tang F., Adrenomedullin in the rat testis. I: its production, actions on testosterone secretion, regulation by human chorionic gonadotropin, and its interaction with endothelin 1 in the, Leydig cell.Biol Reprod. 2008, 78: 773-9.

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of Adrenomedullin and Its Receptors in the Reproductive System of the Rat, Effects on Steroid Secretion in Rat Ovary,Biol Reprod. 2008, 79:200.

 

Li Y.Y., Li L., Hwang I.S.S., Tang F. and O W.S., Coexpression of adrenomedullin and its receptors in the reproductive system of the rat: Effects on steroid secretion in rat ovary, Biology of Reproduction. 2008, 79: 200-208.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat., The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. 2007, 62: 1346-1351.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat, J Gerontol A Biol Sci Med Sci. 2007, 62: 1346-51.

 

Li Y.Y., O W.S. and Tang F., Effect of aging on the expression of adrenomedullin and its receptor component proteins in the male reproductive system of the rat, Journal of Gerontology: Biological Sciences. 2007, 62A(12): 1346-1351.

 

O W.S., Li S.L. and Tang F., Adrenomedullin in female reproduction: folliculogenesis, luteogenesis and pregnancy, Society for Reproduction and Fertility Abstract Series June 2008. 74 P62.

 

Researcher : Tang WH

List of Research Outputs

 

Tang W.H., Wu S., Wong T.M., Chung S.K. and Chung S.S.M., Polyol Pathway Mediates Iron-induced Oxidative Injury In Ischemic-reperfused Rat Heart, Free Radical Biology Medicine. 2008, 45: 602-10.

 

Researcher : Tjong YW

List of Research Outputs

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Melatonin ameliorates hippocampal nitric oxide production and large-conductance calcium-activated potassium channel activity in chronic intermittent hypoxia, Journal of Pineal Research. 2008, 44: 234-243.

 

Tjong Y.W., Li M., Hung M.W. and Fung M.L., Nitric oxide deficit in chronic intermittent hypoxia impairs large conductance calcium-activated potassium channel activity in rat hippocampal neurons, Free Radical Biology and Medicine. 2008, 44(4): 547-557.

 

Researcher : Tse YC

List of Research Outputs

 

Chan Y.S., Tse Y.C., Lai S.K., Lai C.H. and Yung W.H., Developmental changes in excitatory and inhibitory transmission at central vestibular synapses of rats. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007.

 

Chan Y.S., Lai C.H., Lai S.K., Li C., Ma C.W. and Tse Y.C., The differential role of glutamate receptors in postnatal spatial recognition. Journal of Neurochemistry, 106 Suppl: 6, 8th Biennial Meeting of Asia-Pacific Society for Neurochemistry. Shanghai, China. June 24-27. 2008.

 

Lai C.H., Yiu C.N.S., Tse Y.C. and Chan Y.S., Functional maturation of vestibular-related brainstem neurons: Postsynaptic currents and responsiveness to horizontal rotational-/translational head movements, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, # POS-SAT-280.

 

Ng K.P., Lai C.H., Tse Y.C. and Chan Y.S., Thalamic neurons detect both rotational and translational motions in the horizontal plane. Neuroscience Research 58: S99, 30th Annual Meeting of Japan Neuroscience Society. Yokohama. Sept. 10-12. 2007, 58.

 

Tse Y.C., Lai C.H., Lai S.K., Liu J.X., Yung K.K.L., Shum D.K.Y. and Chan Y.S., Developmental expression of NMDA and AMPA receptor subunits in vestibular nuclear neurons that encode gravity-related horizontal orientations. , Journal of Comparative Neurology. 2008, 508: 343-364.

 

Researcher : Wong TM

Project Title:

Signaling mechanism of preconditioning with U50, 488H in the rat - the role of Katp channels

Investigator(s):

Wong TM

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2002

 

Abstract:

To determine the roles of two types of KATP channels, mitochondrial and sarcolemmal in delayed cardioprotection of preconditioning with a [kappa] opioid receptor [kappa]-OR agonist, U50, 488H; to determine the relationship between KATP channels and heat shock protein 70 (HSP70), which has already been shown to mediate delayed cardioprotection of preconditioning with U50, 488H.

 

Project Title:

Effects of testosterone on intracellular Ca2 transient and its response to adrenoceptor stimulation

Investigator(s):

Wong TM

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

11/2004

 

Abstract:

To understand the role of T on the regulation of cardiac functions; to understand the interaction between T and ARs.

 

Project Title:

Cardioprotective effect of oestrogen–Ca2+ homeostasis and the signalling mechanisms

Investigator(s):

Wong TM

Department:

Physiology

Source(s) of Funding:

Small Project Funding

Start Date:

01/2006

 

Abstract:

Eostrogen (E), the predominant female sex hormone, is well known to protect the heart against ischaemic insults. We showed in our previous studies that E confers cardioprotection by suppressing the beta1-adrenoceptor (b1-AR). We also found in preliminary studies that the cardioprotection resulting from interaction (cross-talk) between E and 1-AR is accompanied by attenuation of intracellular Ca overload, indicating that the cross-talk may restore the impaired Ca homeostasis. The first objective of the proposed study is to determine the effects of E on Ca handling at different sites of the ventricular myocyte. Since Ca homeostasis is regulated in aprt by two signalling pathways, namely Gs-protein/adenylyl cyclase/cAMP/protein kinase A and Ca2+/calmodulin kinase II (CaMKII), we also propose to determine whether and how estrogen affects Ca homeostasis via thse two pathways. With the support from the University and the Research Grants Council, the PI has been working on role of estrogen in cardiac function and cardioprotection in the past few years. The proposed study is a contimuation of the existing research. So the PI has all the expertise for the proposed study in terms of facility and knowledge in the field of study.

 

List of Research Outputs

 

Li R., Wong G.T.C., Wong T.M., Zhang Y. and Irwin M.G., Intrathecal Morphine preconditions the heart via kappa, delta and mu opioid receptors, World Congress of Anaesthesiology, Cape Town. 2008.

 

Li Y., Yu C.K., Wong G.T.C., Wong T.M. and Irwin M.G., Remifentanil preconditioning confers delayed cardioprotection in the rat, British Journal of Anaesthesia. UK, 2007, 99: 632 - 638.

 

Tang W.H., Wu S., Wong T.M., Chung S.K. and Chung S.S.M., Polyol Pathway Mediates Iron-induced Oxidative Injury In Ischemic-reperfused Rat Heart, Free Radical Biology Medicine. 2008, 45: 602-10.

 

Wu W.K., Li G.R., Wong T.M., Wang J.Y., Yu L. and Cho C.H., Involvement of voltage-gated K(+) and Na (+) channels in gastric epithelial cell migration. , Mol Cell Biochem. 2008, 308(1-2): 219-26.

 

Researcher : Wu S

List of Research Outputs

 

Tang W.H., Wu S., Wong T.M., Chung S.K. and Chung S.S.M., Polyol Pathway Mediates Iron-induced Oxidative Injury In Ischemic-reperfused Rat Heart, Free Radical Biology Medicine. 2008, 45: 602-10.

 

Researcher : Yeung HM

List of Research Outputs

 

Yeung H.M., Hung M.W. and Fung M.L., Cardioprotective effect of melatonin on impaired calcium homeostasis, myocardial and ischemia-reperfusion injuries in chronically hypoxic rats, The 11th Annual Scientific Meeting of the Institute of Cardiovascular Science and Medicine (Journal of the Hong Kong College of Cardiology). 2007, 15(2):88: 10.

 

Yeung H.M., Hung M.W. and Fung M.L., Melatonin ameliorates calcium homeostasis in myocardial and ischemia-reperfusion injury in chronically hypoxic rats, 12th Research Postgraduate Symposium, HKU. 2007, 1-39.

 

Yeung H.M., Hung M.W. and Fung M.L., Melatonin ameliorates impaired calcium homeostasis, myocardial and ischemia-reperfusion injuries by preserving the sarcoplasmic reticulum calcium handling proteins and anti-oxidant enzymes in intermittent hypoxic rats, The Third International Symposium on Healthy Aging, HKU, Hong Kong. 2008, 15.

 

Researcher : Yiu CNS

List of Research Outputs

 

Lai C.H., Yiu C.N.S., Tse Y.C. and Chan Y.S., Functional maturation of vestibular-related brainstem neurons: Postsynaptic currents and responsiveness to horizontal rotational-/translational head movements, Seventh IBRO World Congress of Neuroscience, Melbourne, Australia, July 12-17. Melbourne, Australia, 2007, # POS-SAT-280.

 

Lai C.H., Yiu C.N.S. and Chan Y.S., Postnatal development of canal-related brainstem neurons responsive to three-dimensional rotations in rats, Abstracts of Third International Symposium on Healthy Aging - Improving the Health of an Aging Population: 23. March 1-2. 2008.

 

Lai C.H., Yiu C.N.S. and Chan Y.S., Postnatal development of canal-related brainstem neurons responsive to vertical head rotation in rats. Neuroscience Bulletin 23: 83, 7th Biennial Conference of Chinese Society for Neuroscience. Hangzhou, China. Oct. 24-28. 2007.

 

Researcher : Yue J

List of Research Outputs

 

Yue J., The mechanism of mitotic activation of Plk1 in Xenopus egg extracts. , The 2007 Stohlman Scholar Symposium of Leukemia and Lymphoma Society, Anaheim, California, November 8-9, 2007.. 2007.

 

Researcher : Yuen YL

List of Research Outputs

 

Yuen Y.L., Kwok J.C.F., Lau J.W.K., Chan Y.S. and Shum D.K.Y., Commissural projection from the vestibular nucleus in rat embryos: role of chondroitin sulfates, Soc. Neuroscience Abstract (U.S.A.): 870.6. 2007.

 

Researcher : Zhang X

List of Research Outputs

 

Zhang X., Li G.R. and Bourreau J.P., The effect of adrenomedullin on the L-type calcium current in myocytes from septic shock rats: Signaling pathway. , Am J Physiol Heart Circ Physiol. 2007, 293(5): H2888-93.



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