|
Researcher
: Cao K |
|
List of Research Outputs |
|
Cao K., 補腎、健脾、活血中藥對環磷醯胺造模小鼠骨髓抑制影響的實驗研 究 , 第二屆粵港澳腫瘤大會學術論文集, 廣州, 2007, 171-173. |
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Chen R.Q., Cao K., Lam T.H. and Wong C.M., Symptom characteristics of Kidney-Yin deficiency and Kidney-Yang deficiency in Hong Kong Chinese midlife women, Journal of Alternative and Complementary Medicine. 2008, 14(5): 457-460. |
|
Chen
R.Q., Wong C.M., Cao K. and Lam T.H., The Development Of A
Questionnaire For Diagnostic Evaluation Of Kidney-Yin Deficiency And
Kidney-Yang Deficiency, In: Felix Wong, Basil Roufogalis , Third
International Congress on Complementary Medicine Research. |
|
Chen
R.Q., Lam C.L.K., Wong C.M., Cao K. and Lam T.H., The HRQoL measured by SF |
|
Chen
R.Q., Wong C.M., Cao K. and Lam T.H., The measurement properties of a
kidney deficiency syndrome questionnaire in Hong Kong Chinese midlife women
(abstract and oral presentation), 12th Research Postgraduate Symposium, 12 & 14
December 2007, Hong Kong. |
|
Researcher
: Chen H |
|
List of Research Outputs |
|
Chen
H., Cho W.C.S., Sze C.W. and Tong Y., A Systematic Review on Efficacy
of Erxian Decoction Treated Menopausal Symptoms, 3rd International
Congress Complementary Medicine Research. |
|
Chen H., Cho W.C.S., Sze C.W. and Tong Y., Treatment of Menopausal Symptoms with Er-xian Decoction: A Systematic Review, America Journal of Chinese Medicine. 2008, 36(2): 233-244. |
|
Researcher
: Chen J |
|
Project Title: |
The effects of quickening blood circulation & transforming blood stasis on feminine tumours by Chinese herbs (DC0618) |
|
Investigator(s): |
Chen J, Chan YS |
|
Department: |
|
|
Source(s) of Funding: |
Small Project Funding |
|
Start Date: |
01/2006 |
|
Abstract: |
|
Purpose: This study is designed to evaluate the properties of treatment for feminine tumours such as ovarian and breast tumours by quickening blood circulation & transforming blood stasis. The designed prescription has been named DC0618 which consists of Chinese herbs that work by the above approach. Issues/Problems：These prescriptions have been widely used for the prevention and treatment of tumours. A lot of articles have reported that these herbs can inhibit tumour cell growth by releasing cytotoxin; inducing tumour cell apoptosis; lowering blood viscosity; improving micro-circulation and modulating immune system etc. Many researchers believe that herbal compounds do not have direct cytotoxic effects [8]. Rather, the anticancer properties of these herbs are thought to be related to the activation and release of cytokines that then induce apoptosis [9]. Apoptosis is usually regulated tightly and its execution may be initiated by many different signals, either from within or outside the cell [10]. While different genes such as p53, c-myc or Bcl-2/Bax have been shown to shift the balance from cell survival to cell death, apoptosis may also be modulated by signals from ligand-receptor interactions, as has been shown for TNF-/TNF-receptor [11, 12]. It is well documented that cytotoxic drugs exert their effects through the induction of apoptosis [13]. |
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List of Research Outputs |
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Chen J. and Loo T.Y., Immunomulatory effect of YPF on human lymphocytes in vitro , The 6th biennial meeting of the Asian breast cancer society . 2007. |
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Chen J., Loo T.Y. and Chou W.K., Juzen-taiho-to enhances immune activity in vitro and minimizes cyto-toxicity in vivo, the 6th Biennial meeting of Asian Breast cancer society. 2007. |
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Chen J., WDY' conference , Breast cancer treatment strategy in Chinese medicine. 2007. |
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Chen J., prevention and treatment Breast cancer by Chinese Medicine , 2007TWGHs Eddie wang symposium on integrated chinese and western Medicine. 2007. |
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Chen J., treatment strategy of Chinese medicine on Breast caner, WDY's academically conference . 2007. |
|
Diao J J., Chen J. and Lao Y J., 柴胡用治发热的临床应用概况, 廣州中醫藥大學學報, Guanzhou, 2008, 3(25)2008: 259. |
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Li Y., Chen J. and Loo T.Y., Immunomodulatory effect of Yupingfeng (YPF) on human lymphocytes in vitro, Organisation for Oncology and Translational Research (OOTR) 4th Annual Conference. 2007. |
|
Li Y., Loo T.Y., Guan X.Y. and Chen J., anticancer effect if Dioscin in breast and esopageal cancer cells, the 6th Biennial meeting of Asian Breast cancer society . 2007. |
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Loo T.Y., Chen J. and Chow W.C., A 3-year Prospective Study in Monitoring Bone Mineral Density of Mandible in 180 Fully Edentulous Patients after chemotherapy., the 6th Biennial meeting of Asian Breast cancer society . 2007. |
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Loo T.Y., Chen J. and Chow W.C., A non-toxic herbal remedy which enhance lymphocyte activity and cytokine secretion: Takayama Ganoderma Lucidum, the 6th Biennial meeting of Asian Breast cancer society . 2007. |
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Loo T.Y., Chen J. and Chow W.C., Traditional Chinese medicine, Rhodia, prevents oral membrane ulceration in patients undergoing chemotherapy: a novel discovery, the 6th Biennial meeting of Asian Breast cancer society . 2007. |
|
Wang D.N., Lin S.H., Yang D.P., Jin J., Tong Y. and Chen J., Characterization of
steroidal saponins in crude extract from Dioscorea Nipponica Makino by liquid
chromatography tandem multi-stage mass spectrometry, Analytica Chimica
Acta. |
|
Wang S., Yang X., jiang S., Chen J., houng S. and Lu F., 弓形虫感染宫内垂直传播的免疫特点, 热带医学杂志, guanzhou, 热带医学杂志, 2008, 8(5): 505. |
|
sui L., Li J.M., wang D.M., zhu L.P., Chen J. and Yang D., 应用QuikPrep HSCCC 系统 实现共轭亚油酸及其异构体的分离, 分析试验室, 2008, 27: 253. |
|
Researcher
: Chen RQ |
|
Project Title: |
Developing a novel scientific approach for clinical research of Chinese medicine using menopause as a model |
|
Investigator(s): |
Chen RQ |
|
Department: |
|
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
02/2005 |
|
Abstract: |
|
B) To analyse gene expression in subjects with KDS-Yin and KDS-Yang, who are identified with the validated KDS Scale. (Note: KDS-Yin: A pattern of pathogenesis in Chinese medicine: Kidney-Yin-deficiency syndrome KDS-Yang: A pattern of pathogenesis in Chinese medicine: Kidney-Yang-deficiency syndrome) |
|
List of Research Outputs |
|
Chen R.Q., Clinical evaluation of classic theoretical literatures on Kidney-deficiency syndromes, 腎虛證相關經典文獻理論的初步臨床驗證, The Chinese University of Hong Kong School of Chinese Medicine. 2008. |
|
Chen R.Q., Cao K., Lam T.H. and Wong C.M., Symptom characteristics of Kidney-Yin deficiency and Kidney-Yang deficiency in Hong Kong Chinese midlife women, Journal of Alternative and Complementary Medicine. 2008, 14(5): 457-460. |
|
Chen
R.Q., Wong
C.M., Cao K. and Lam T.H., The Development Of A
Questionnaire For Diagnostic Evaluation Of Kidney-Yin Deficiency And
Kidney-Yang Deficiency, In: Felix Wong, Basil Roufogalis , Third International
Congress on Complementary Medicine Research. |
|
Chen
R.Q., Lam
C.L.K., Wong C.M., Cao K. and Lam T.H., The HRQoL measured by SF |
|
Researcher
: Chu |
|
List of Research Outputs |
|
Chu O.M., <中醫藥治療頭頸部腫瘤>的講座, "農本方"特約新城電台節目:"中醫藥透視", 2007. |
|
Researcher
: Feng Y |
|
Project Title: |
Development of Animal models and cell culture for Evaluating the bioactive effect of Chinese Medicines Series1: bioactive Screening and Estimation of Chinese Medicines by using rats with hepatic damage models (including liver cancer models), rats with renal disease models and relevant cell culture |
|
Investigator(s): |
Feng Y, Tsao GSW |
|
Department: |
|
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
11/2005 |
|
Abstract: |
|
Chinese medicines have thousands of
years’ history of use in |
|
List of Research Outputs |
|
Feng
Y., Luo W.Q. and Zhu S.Q., Explore new
clinical application of Huanglian and corresponding compound prescriptions
from their traditional use. , In: Editor-in-Chief: |
|
Feng
Y., HKU TCM workshop 2007, Coptidis
Rhizoma: new drug for liver diseases?. |
|
Feng
Y., Linnaeus and medicinal products. An
international conference on drugs of natural origin in the honour of Carl
Linnaeus. , The misuse of Aristolochia-related species (ARSs) due to
confused herbal names in |
|
Feng Y., Tsao G.S.W., Tong Y. and Ng K.M., Matching Grant (4th Phase)-Research project on alternative drug for bear bile, UGC (Government Matching Grant Scheme). 2008, HK$402,500. |
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Feng Y., Tsao G.S.W., Tong Y. and Ng K.M., Research project on alternative drug for bear bile, Yiqingzhai Foundation (The Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine). 2008, HK$805,000. |
|
Feng Y. and Tong Y., 从中药现代研究看中医学的基本特点, 香港中医杂志. Hong Kong, 香港中医杂志出版部, 2008, 3(1): 17-21. |
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Feng Y., Ye X. and Tong Y., 建立香港中藥不良反應監測系統和發揮中醫藥在香港藥物濫用防治中的作用, 中國藥物濫用防治雜誌. Beijing., Publisher of Chinese Journal of Drug Abuse Prevention and Tr, 2007, 13(4): 220-223. |
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Feng Y., 中華中醫中藥促進會註冊中醫學術講座., 古今縱橫: 黃連及其複方., 2008. |
|
Feng
Y., 中國中醫中藥行: 2007(香港)國際中醫中藥高級論壇, 從中藥現代研究看中醫學的基本特點, |
|
Feng
Y., 香港註冊中醫學會學術講座, 中藥不良反應和中毒概述, |
|
Tang
J., Feng Y., Tong Y., Jia R., Sy L.K. and Man R.Y.K., The Mucilage Cavity is a
Distinct Microscopic Characteristic Showing in the Phloem but not Pith of
Rhizomes for the Identification of Radix et Rhizoma Rhei., In: Editoral
Department of Wuhan University Journal of Natural Sciences, Wuhan
University Journal of Natural Sciences. |
|
Ye X.,
Feng Y., Tong Y. and Tsao G.S.W., The effects of coptis
extract on hepatoprotection in rats with liver damage., 2007 Hong
Kong-Macau postgraduate Symposium on Chinese Medicine. |
|
Zhang Y., Tong Y., Sze C.W., Feng Y., Song J.X., Wong R.N.S. and NG T.B., Application of sequence characterized amplified region (SCAR) analysis to authenticate Lycium barbarum and its adulterants., In: Stephen Sze CW, Song JX, Ricky Wong NS, Ng TB, Tong Y, Kalin Zhang YB. , Biotechnology and Applied Biochemistry . 2007, 51: 15-21. |
|
Researcher
: Jia R |
|
List of Research Outputs |
|
Tang
J., Feng Y., Tong Y., Jia R., Sy L.K. and Man R.Y.K., The Mucilage Cavity is a
Distinct Microscopic Characteristic Showing in the Phloem but not Pith of
Rhizomes for the Identification of Radix et Rhizoma Rhei., In: Editoral
Department of Wuhan University Journal of Natural Sciences, Wuhan
University Journal of Natural Sciences. |
|
Researcher
: Lau ASY |
|
Project Title: |
Mechanism of SARS-coronavirus pathogenesis - identification of viral genes implicated in immune dysregulation |
|
Investigator(s): |
Lau ASY, Peiris JSM |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
VCO SARS Research Fund |
|
Start Date: |
07/2003 |
|
Abstract: |
|
To investigate the pathogenesis of SARS-Coronavirus-induced pulmonary injury and multi-organ dysfunctions in humans: (a) to identify the viral determinants of proinflammatory cytokine induction: candidate envelope genes to be examined include Spike (S), Envelope (E) and Membrane (M); (b) to investigate the signaling pathways including kinases and transcription factors involved in the cytokine induction. |
|
Project Title: |
A role for apoptogenic gene PKR in regulating TNF and cytokine expression in Avian influenza H5N1 infections |
|
Investigator(s): |
Lau ASY, Peiris JSM |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
|
Start Date: |
10/2003 |
|
Abstract: |
|
To investigate whether H5N1/97 is a potent inducer of specific signaling kinases, compared to H1N1 or H3N2, leading to enhanced cytotoxicity in the host cell; to investigate whether H5N1/97 induction of TNF-[alpha] and related proinflammatory cytokines is dependent on the expression of these signaling kinases; the mechanism of H5N1/97-induced TNF-[alpha] and related cytokine expression; to identify transcription factors (TF) operative in H5N1-induced and kinase-mediated pathways; the role of these signaling kinases in H5N1/97-induced cytotoxicity: to identify kinase-regulated downstream pathways and induction of apoptogenic genes. |
|
Project Title: |
Immune dysregulation in HIV infection - mechanism of mycobacterial pathogenesis |
|
Investigator(s): |
Lau ASY |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
|
Start Date: |
01/2005 |
|
Abstract: |
|
To confirm the role of HIV tat on monocytic cells in TH2 cytokine induction and TH1 reponse suppression; to identify the signaling pathways and transcription factors operative in Tat activation of IL-10 promoter; to investigate the effects of BCG or mycobacterial cell wall submits on IL-10 induction; to examine whether Tat interacts with TLR-associated kinases and factors directly or via PKR; to delineate whether exogenous IFN-[gamma] and other TH1 cytokines can counteract the effects of Tat and MTB in IL-10 induction. |
|
Project Title: |
Immunotherapy of mycobacterial infections: a cell and molecular model |
|
Investigator(s): |
Lau ASY, Lee DCW |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
Research Fund for the Control of Infectious Diseases - Full Grants |
|
Start Date: |
02/2005 |
|
Abstract: |
|
To examine the profile of cytokine
expression including proinflammatory cytokines and anti-inflammatory cytokines
in BCG and mycobacterial cell wall subunits-treated cells; to confirm the
role of PKR and related kinases in signaling pathways operative in the BCG-
and cell wall subunit-induced cytokine expression; to examine the role of IL |
|
Project Title: |
Cytokine Dysregulation and Virus-Induced Cell Death in Avian Influenza Virus Infections |
|
Investigator(s): |
Lau ASY, Peiris JSM |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
Small Project Funding |
|
Start Date: |
11/2005 |
|
Abstract: |
|
Objectives: In this project, we will use
H9N2 as prototypes to examine the cellular effects of TNF and IFN induction
by avian influenza viruses. We will delineate whether there is differential
induction of cytotoxicity by the avian viruses from the human ones. We will
also examine the consequences of simultaneous induction of TNF and IFN on
cell survival and expression of apoptotic genes. Our Specific Aims are 1) We
will delineate whether there is a differential induction of cytotoxicity by
the avian viruses (H9N2) compared to the human ones (H1N1 or H3N2). 2) We
will examine the consequences of simultaneous induction of TNF and IFN on
cell survival and expression of apoptotic genes. Background Influenza A virus
infects a wide range of species, including poultry, swine, horses, seals and
humans. Of the 15 hemagglutinins (HA) subtypes of influenza A, only H1, H2
and H3 had been known to cause significant morbidity and mortality in humans
(1-3). The 1997 outbreak of avian influenza H5N1/ |
|
Project Title: |
Mechanisms of inteferon dysregulation and virus-induced cell death in avian influenza H5N1 virus infections |
|
Investigator(s): |
Lau ASY, Lee DCW |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
Research Fund for the Control of Infectious Diseases - Full Grants |
|
Start Date: |
10/2006 |
|
Abstract: |
|
To examine the signaling pathways in IFN-beta super-induction by avian influenza viruses including H5N1/97, as compared to human viruses including H1N1; to investigate whether there is a preferential activation of IFN regulatory factors (IRFs) during IFN induction in avian virus infections; to determine the transcriptional activation of IFNs by electrophorectic mobility shift, IFN promoter-driven luciferase and steady-state quantitative-PCR assays, in addition to Western blots; to delineate whether there is differential induction of cytotoxicity and apoptotic genes by the avian viruses from the human ones. |
|
Project Title: |
HIV dysregulation of the toll-like receptor system - implications for pathogenesis |
|
Investigator(s): |
Lau ASY |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
|
Start Date: |
10/2006 |
|
Abstract: |
|
To investigate the effects of Tat on the
expression of TLR |
|
Project Title: |
Regulation of Matrix Metalloproteinases by Epstein-Barr Virus Latent Membrane Protein 1 |
|
Investigator(s): |
Lau ASY, Chua DTT, Lee DCW |
|
Department: |
Paediatrics & Adolescent Med |
|
Source(s) of Funding: |
Small Project Funding |
|
Start Date: |
11/2006 |
|
Abstract: |
|
Objectives: In this project, we will
investigate the role of Epstein-Barr Virus Latent Membrane Protein 1 (LMP1)
in the expression of Matrix Metalloproteinases (MMPs) and their consequent
effects on cell migration. Our Specific Aims are: 1) We will generate
LMP1-expressing cells including fibroblasts or epithelial cells by transient
transfection of the cells with LMP1 cDNA. 2) We will delineate the mechanisms
of Epstein-Barr Virus, specifically LMP1 gene, in the regulation of MMPs
induction and the promotion of cell invasion. Background Epstein-Barr Virus
(EBV) is a human herpesvirus that infects a large percentage of the world’s
population (1). EBV has been known to be highly associated with human
malignancies, including Burkitt’s lymphoma, post-transplant lymphomas,
AIDS-associated lymphoma, gastric carcinoma, and the highly invasive
nasopharyngeal carcinoma (NPC), putatively related to and as a consequence of
the latent infection state (1,2). Specific EBV latent genes including LMP1, |
|
List of Research Outputs |
|
Rong J., Tilton R., Shen J., Ng K.M., Liu C., Tam P.K.H., Lau A.S.Y. and Cheng Y.C., Genome-wide Biological Response Fingerprinting (BioReF) of the Chinese Botanical Formulatrion ISF-1 Enables the Selection of Multiple Marker Genes as a Potential Metric for Quality Control , Journal of Ethno-Pharmacology . 2007, 113: 35-44. |
|
Rong J., Cheung C.Y.H., Lau A.S.Y., Shen J., Tam P.K.H. and Cheng Y.C., Induction of heme oxygenase-1 by traditional Chinese medicine formulation ISF-1 and its ingredients as a cytoprotective mechanism against oxidative stress, International Journal of Molecular Medicine. 2008, 21(4): 405-411. |
|
Sze C.W., Zhang Y., Lau A.S.Y. and Tong Y., Developing method to screen for endocrine-active Chinese medicine formulation to relieve the menopausal syndrome, Sixth Meeting of Consortium for Globalization of Chinese Medicine (Poster). Beijing, 2007, Bio-028: P.95. |
|
Sze C.W., Zhang Y., Lau A.S.Y. and Tong Y., Erxian Decoction Stimulates Estrogen Secretion Via Steroidogenic Signaling Transduction Pathway In Vivo., 3rd International Congress Complementary Medicine Research . Sydney, Australia, 2008. |
|
Sze C.W., Lau A.S.Y., Zhang Y., Tong Y. and Zhang Z., Mechanical Study of Erxian Decoction for Peri-menopausal Syndrome., Third International Symposium on Healthy Aging: Improving the Health of an Aging Population. (Speaker). 2008. |
|
Tong Y., Sze C.W., Zhang Y., Zhang Z. and Lau A.S.Y., "Mechanical Study of Erxian Decoction for Peri-menopausal Syndrom”, Third International Healthy Aging - Improving the Health of an Aging Population”, Hong Kong (Speaker). 2008. |
|
Researcher
: Li H |
|
List of Research Outputs |
|
Li H., Sze C.W. and Tong Y., Immunomodulation Induced By Chinese Medicine Herb, Rhodiola algida, On Th1- and Th2-dependent Cytokine Production., 3rd International Congress Complementary Medicine Research (Poster Abstract, p118). Sydney, Australia, 2008. |
|
Shi J., Tong Y., Shen J. and Li H., Effectiveness and safety of herbal medicines in the treatment of irritable bowel syndrome: A systematic review., World Journal of Gastroenterology. 2008, Iss. 14(3): 454-462. |
|
Researcher
: Li H |
|
List of Research Outputs |
|
Li H., Sze C.W. and Tong Y., Immunomodulation Induced By Chinese Medicine Herb, Rhodiola algida, On Th1- and Th2-dependent Cytokine Production., 3rd International Congress Complementary Medicine Research (Poster Abstract, p118). Sydney, Australia, 2008. |
|
Shi J., Tong Y., Shen J. and Li H., Effectiveness and safety of herbal medicines in the treatment of irritable bowel syndrome: A systematic review., World Journal of Gastroenterology. 2008, Iss. 14(3): 454-462. |
|
Researcher
: Li L |
|
List of Research Outputs |
|
Li L., The Different Effects of Electro-acupuncture at Different Time on The plasma cAMPs, cGMPs and cAMP／cGMP ratios of Normal Young Adults , Chinese-French & Chinese-European International Forum of Chronomedicine. 2007. |
|
Li L., 穴位別講, 香港大學中醫藥學院主辦龐鼎元系列中醫專題講座, 2007. |
|
Li L., 《黃帝內經》、《素問》、《靈樞》諸書名的文化內涵, 香港《黃帝內經》學術研討會, 2008. |
|
Researcher
: Li Y |
|
List of Research Outputs |
|
Li Y., Chen J. and Loo T.Y., Immunomodulatory effect of Yupingfeng (YPF) on human lymphocytes in vitro, Organisation for Oncology and Translational Research (OOTR) 4th Annual Conference. 2007. |
|
Li Y., Loo T.Y., Guan X.Y. and Chen J., anticancer effect if Dioscin in breast and esopageal cancer cells, the 6th Biennial meeting of Asian Breast cancer society . 2007. |
|
Researcher
: Liu C |
|
Project Title: |
Systematic Determination and Compilation of DNA Barcodes for Medicinal Plants Used In Traditional Chinese Medicines |
|
Investigator(s): |
Liu C |
|
Department: |
Medical Faculty |
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
02/2006 |
|
Abstract: |
|
During the past decades, interest in Traditional Chinese Medicine (TCM) has grown worldwide. Among the many barriers for TCM to enter the mainstream medicines is the inconsistent quality of TCMs. This complicating factor has hindered the research, development and testing of TCMs. As a result, recent guideline from the US Food and Drug Administration has outlined the required quality control measures (www.fda.gov/cder/guidance/4592fnl.htm) for botanical raw materials (that is, fresh or processed part of a single species of plant or a fresh or processed alga or macroscopic fungus), botanical drug substances (that is, materials derived from one or more plants, algae, or macroscopic fungi by certain processes.) and botanical drug products (that is, a finished, labeled product that contains vegetable matter processed from one or many botanical substance). Among all these quality control requirements, the first step is to make sure that the correct botanical species have been used in the formulation. Unfortunately, there is no universal standard species determination method available at present time. Our hypothesis is that a short DNA sequence (100-650bp long) can be used for species identification of medicinal plants. This kind of short DNA sequence is called a DNA barcode in the following text. Our long term goal is to (1) systematically determine DNA barcode(s) that can be used for the species identification of most, if not all, medicinal plants used in the TCMs, and compile them into a DNA barcode library; and (2) establish a standard procedure to amplify the barcode sequence(s) from a test sample and compare it to sequences from the DNA barcode library for species identification. The present study is a pilot study in an attempt to lay the ground for the future large scale DNA barcode identification project in all important medicinal plants used in TCM. This study intends to address the problem of misidentification of botanical raw materials that has been well-reported in the literature and media. For example, a “Siberian Ginseng” (Eleutherococcus Senticosus) product implicated in a case of neonatal androgenisation was found upon analysis to be an unrelated species, Chinese silk vine “Periploca Sepium” (1). In Hong Kong, encephalopathy and neuropathy associated with a Chinese herbal preparation purportedly made from the roots of Long-Dan-Cao (Gentiana Regescens) turned out to be due to another plant Podophyllum Emodi. In another example, more than 48 cases of renal poisoning attributed to fang-ji (Stephania Tetrandra) in weight-loss preparation were actually caused by Guang-Fang-Ji (Aristolochia Fangchi); aristolochic acid is a known nephrotoxin (2). The exactly extent of this problem can be much greater than what have been reported considering that only the cases having the most severe consequences would be reported. The result obtained from this study will lay the foundation for the development of a standard method that will eventually limit, if not able to eliminate the problems of misidentification, adulteration and contamination of botanical raw materials and botanical drug products. We plan to accomplish our goal through three specific aims: SPECIFIC AIM 1: To select the DNA sequence that can be used as DNA barcode for species determination of medicinal plants. Recent study has led to the concept of “DNA barcode”, which is a short to very short (100-650bp) sequence that server as a DNA marker or fingerprint and has the power to identify most, if not all organisms on Earth based on variability in the barcode sequences (3). An international consortium has been formed to systematically barcode life in different domains (http://barcoding.si.edu/index_detail.htm). A proof-of-concept study has showed that the cytochrome c oxidase I (COI) sequences can server as the core of a global bio-identification system for many animals. These studies suggest that using a single DNA barcode for species identification of medicinal plants is also possible. With very few sequence information available for most medicinal plants, we will depend on literature search to select the sequence most likely to represent the DNA barcode. SPECIFIC AIM 2: To establish a standard experimental procedure for species determination of medicinal plants under test. The procedure will mainly involve Polymerase Chain Reaction (PCR) following by automatic DNA sequencing. Although the standard procedure of PCR has been well established, we might still encounter several problems since we are trying to amplify DNA from samples containing botanical species belonging to a wide range of taxonomic groups. First, our PCR primers might not be universal enough to effectively and specifically amplify the intended DNA barcode from certain samples. Second, the DNA purification method might require substantial modification to extract DNA from certain botanical samples. Third, specific measures might have to be taken to remove potential DNA polymerase inhibitors in the botanical samples. Consequently, significant amount of efforts are expected to optimize the experimental conditions. The goal of this aim to establish a procedure that can be used in the future large scale project. SPECIFIC AIM 3: To build a pilot database of DNA barcodes from approximately two hundred medicinal plants. Using DNA fingerprinting for species identification in specific medicinal plants have been reported before. However, systematic efforts to identify universal DNA barcodes and to use these barcodes for species determination and identification have not been reported. After the successful completion of specific aim 1 and 2, we will extend the study to approximate 200 medicinal plants. This survey type of study will help us to evaluate the usefulness and robustness of the selected DNA barcode and the standard procedure developed above. This information will facilitate the design of the following large-scale DNA barcode determination project. In summary, this proposed study intends to carry out the pilot study that will eventually solve the practically critical problem of species identification of medicinal plants. The data generated from this study can have broad applications in a wide range of research areas. For example, it will generate important new insights into the diversification of life and the rules of molecular evolution. In addition, it will lay the ground work for the future development of a systematic, reliable, cost-effective and accessible solution to authenticate the botanical materials used for making TCMs. |
|
Project Title: |
Identification and characterization of chromosomal functional domains in completely sequenced genomes |
|
Investigator(s): |
Liu C, Lau ASY |
|
Department: |
Medical Faculty |
|
Source(s) of Funding: |
Competitive Earmarked Research Grants (CERG) |
|
Start Date: |
08/2006 |
|
Abstract: |
|
(1) To develop statistical methods/software tools that can identify clusters of adjacent genes sharing similar expression patterns based on data derived from whole genome mRNA expression profiling experiments; (2) To apply these methods and tools to identify and characterize chromosomal functional domains in the genomes of lower and higher eukaryotes, and to build a Functional Domain Database to store these results; (3) To apply the tools and results obtained from (1) and (2) to solve specific biological problems. Specifically, the tools and results will be used to : i) identify regulatory elements for individual and all genes in these functional domains; and ii) identify potential chromosomal rearrangements in disease samples, especially those of cancer samples. |
|
Project Title: |
Identification of Chem- and Bio- Markers of Fritillaria Species Using High Performance Liquid Chromatography, Microarray and Multivariate Data Analysis Methods: Principle Component Analysis and Partial Least Square Modeling |
|
Investigator(s): |
Liu C |
|
Department: |
Medical Faculty |
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
03/2007 |
|
Abstract: |
|
Two of the main challenging tasks in the studies of traditional Chinese medicine (TCM) are (i) to identify the active components of TCM drugs; and (ii) to identify the molecular targets of these active components. These tasks become even more difficult as many TCM drugs exert their therapeutic effects through the coordinated activities of multiple active components on multiple molecular targets. Our long term goal is to develop methodologies to identity the set of active components of TCM drugs and their corresponding set of molecular targets. The determination of these molecules will lay the foundation to (i) elucidate the mechanisms of actions of TCM drugs, (ii) to provide better quality control of already-proven TCM drug products and (iii) to develop novel TCM drug products.Chromatographic and Spectrometric methods have been used extensively to identify the active components of TCM drugs. More recently, with the microarray technology become common place, gene expression profiles have been proposed to be used as markers of biological activities of TCM drugs. Thus, the combinatorial use of chemical profiling and microarray profiling technologies becomes technically feasible. However, many challenging experimental and computational issues remain, for example, (i) how to identify chemo-markers (that is, the set of chemical compounds or active components that contribute to the biological activity of a TCM drug); (ii) how to identify bio-response markers or bio-markers (that is, the set of genes that represent the biological response of the cells to the treatment of a TCM drug, and also represent the biological activity of the TCM drug); (iii) how to quantify the importance of individual component of the chemo- or bio- markers; (iv) how chemo-markers correlate with the bio-markers quantitatively. To our knowledge, systematic study using advance multivariate data analysis methods to address these problems have not been reported. The current study is designed as a very first attempt to address these important problems in a systematic manner.Our hypothesis is that (i) the gene expression profile of bio-markers can be used to quantify the biological activity of a TCM drug and the biological response of cells after treatment; and (ii) the abundance profile of chemo-markers correlate with the expression profile of bio-markers. In this pilot study, we are intended to test the hypothesis and also to establish the necessary methodologies. We will use Fritillaria species as our model TCM herbs. The bulbs of Fritillaria species have been widely used in folk medicines as antitussive and expectorant. We will use the technology of High Performance Liquid Chromatography (HPLC) coupled with Evaporative Light Scattering Detection (ELSD) to generate the chemical compound profiles, which we will use to identify the chemo-markers. On the other hand, we will use Affymetrix GeneChip technology to generate gene expression profiles, which we will use to identify the bio-markers. The identification of chemo- and bio- markers and the determination of their correlations will be carried out using multivariate data analysis methods such as Principle Component Analysis and Partial Least Square Modeling. This pilot study consists of three specific aims: Specific Aim 1: To obtain chemical profiles of Fritillaria species using HPLC-ELSD technology.Specific Aim 2: To obtain gene expression profiles of human cells treated with extracts of Fritillaria species using Affymetrix GeneChip technology.Specific Aim 3: To identify chemo-markers, bio-markers and to determine their correlations using multivariate data analysis methods.If successfully completed, we will extend the proposed study to utilize more sophisticated technologies and more specific assays, and to include more Fritillaria species. For example, we will use the technology of Liquid Chromatography (LC) and Mass Spectrometry (MS) to generate the chemical profiles instead. Real-time PCR, instead of whole genome microarray study, will be used to measure the expression profiles of a list of marker genes identified in the pilot study. Furthermore, more Fritillaria species with greater diversity in chemical compositions and biological activities can be subjected to the above analysis to increate the power of the analysis. These follow-up studies will constitute a RGC grant proposal. |
|
Researcher
: Liu Q |
|
List of Research Outputs |
|
Liu Q., Loo W.T.Y., Sze C.W., Tong Y. and Chow L.W.C., Curcumin’s effect on breast cancer cell – a preclinical trial, The 6th Biennial Meeting of the Asian Breast Cancer Society (ABCS). 2007, 20-22. |
|
Tong
Y., Zhang N.X. and Liu Q.,
Study on the Action Mechanism of "ShuGan LiFei" Therapy on
Experimental Rats with Asthma and under Stress, World Conference of
Stress, |
|
Researcher
: Liu Q |
|
List of Research Outputs |
|
Liu Q., Loo W.T.Y., Sze C.W., Tong Y. and Chow L.W.C., Curcumin’s effect on breast cancer cell – a preclinical trial, The 6th Biennial Meeting of the Asian Breast Cancer Society (ABCS). 2007, 20-22. |
|
Tong
Y., Zhang N.X. and Liu Q.,
Study on the Action Mechanism of "ShuGan LiFei" Therapy on
Experimental Rats with Asthma and under Stress, World Conference of
Stress, |
|
Researcher
: Loo TY |
|
List of Research Outputs |
|
Chen J. and Loo T.Y., Immunomulatory effect of YPF on human lymphocytes in vitro , The 6th biennial meeting of the Asian breast cancer society . 2007. |
|
Chen J., Loo T.Y. and Chou W.K., Juzen-taiho-to enhances immune activity in vitro and minimizes cyto-toxicity in vivo, the 6th Biennial meeting of Asian Breast cancer society. 2007. |
|
Li Y., Chen J. and Loo T.Y., Immunomodulatory effect of Yupingfeng (YPF) on human lymphocytes in vitro, Organisation for Oncology and Translational Research (OOTR) 4th Annual Conference. 2007. |
|
Li Y., Loo T.Y., Guan X.Y. and Chen J., anticancer effect if Dioscin in breast and esopageal cancer cells, the 6th Biennial meeting of Asian Breast cancer society . 2007. |
|
Loo T.Y., Chen J. and Chow W.C., A 3-year Prospective Study in Monitoring Bone Mineral Density of Mandible in 180 Fully Edentulous Patients after chemotherapy., the 6th Biennial meeting of Asian Breast cancer society . 2007. |
|
Loo T.Y., Chen J. and Chow W.C., A non-toxic herbal remedy which enhance lymphocyte activity and cytokine secretion: Takayama Ganoderma Lucidum, the 6th Biennial meeting of Asian Breast cancer society . 2007. |
|
Loo T.Y., Chen J. and Chow W.C., Traditional Chinese medicine, Rhodia, prevents oral membrane ulceration in patients undergoing chemotherapy: a novel discovery, the 6th Biennial meeting of Asian Breast cancer society . 2007. |
|
Researcher
: Mo F |
|
List of Research Outputs |
|
Mo F., Clinical Observation on the Application of 650nm Laser Acupoint Irradiation in the Treatment of the Benign Prostatic Hyperplasia . , 洪文，莫飛智，李建強，趙燕平，曾長春，劉頌豪.650nm鐳射穴位照射治療良性前列腺增生症30例臨床觀察.新中醫，2008，40（1）：60-61, New Journal of Traditional Chinese Medicine.2008，40（1）：60-61. 新中醫, Guangzhou,China, Guangzhou University of Traditional Chinese Medicine, 2008, 40(1): 60-61. |
|
Mo
F., Mo Feizhi. Forum on Teaching of Chinese
Medicine Classical Text "Yellow Emperor Canon of Chinese Medicine",
莫飛智.《黃帝內經》教學探討, Research
papers for the Conference on Research of "Yellow Emperor's Canon of
Chinese Medicine". |
|
Researcher
: Rong J |
|
Project Title: |
Modulation of heme oxygenase-1 expression by traditional Chinese medicine: Implications for antioxidant therapy |
|
Investigator(s): |
Rong J |
|
Department: |
Medical Faculty |
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
07/2006 |
|
Abstract: |
|
Heme oxygenase (HO) is the rate-limiting
enzyme degrading heme into biliverdin, ferrous iron and carbon monoxide.
Among the three isoenzymes isolated to date. HO-1 is highly inducible in
response to diverse stimuli that often provoke oxidative injury to the
tissues/organs [1-4]. The induction of HO-1 expression has been implicated in
a variety of disease states such as atherosclerosis, hypertension, transplant
rejection, acute renal injury, hyperoxia and hypoxia-induced lung injury,
diabetes, cancer, cerebrovascular accident and cardiovascular diseases [5,
6]. Notably, the HO-1 pathway is widely recognized as an intrinsic defense
against numerous pathological challenges. Pharmacological modulation of HO-1
expression therefore offers potential therapeutics to overcome the oxidative
injury and inflammatory tissue damage. Many traditional Chinese medicine
(TCM) products have been claimed to possess antioxidant and anti-inflammatory
activities. However, the mechanisms underlying the actions of TCM products
against the oxidative stress have not been well defined. We have recently
investigated the biological response fingerprints of human liver cells to a
TCM decoction ISF-1, a famous formula widely prescribed to treat ischemia
reperfusion injury. We found for the first time that HO-1 was up-regulated up
to 27-fold by the decoction ISF-1. Moreover, some heme/iron transporters and
iron regulated transcriptional factors were also up-regulated to certain
extent [7]. Based on our preliminary results, we hypothesize that the
heme/iron homeostasis is one of the major targets for the decoction ISF-1.
Presumably, most of the TCM products exert the antioxidant and
anti-inflammatory activities in a similar fashion. Our long term goal is to
investigate and resolve the problem of the mysterious correlation between the
chemical compositions and the therapeutic efficacy of TCM drugs.
Understanding on the molecular mechanisms of TCM actions is the key to
develop evidence-based TCM drugs according to the international standards.
Our strategy fingerprints the biological responses by the powerful
oligonucleotide GeneChips at the genome-wide scale [8, 9]. Based on the
global gene expression profiles, a number of representative mechanisms are
selected to approach the complexity of TCM pharmacological effects.
Subsequently, the active components responsible for the specific pathways are
isolated through the bioactivity-guided fractionation. We anticipate that an
optimal combination of active components (OCAC) will mimic the parent TCM
drug in terms of the potency and efficacy. As a proof of principle, the
proposed study will use a well-known post-stroke rehabilitation formula ISF-1
as an example and focus on ISF-1 mediated regulation of HO-1 pathway, one of
the key pathways in ischemia reperfusion injury [7, 10]. The following
specific aims will demonstrate how we will elucidate the mechanisms of ISF-1
actions and identify the individual active components from the complex
formula. On the basis of the identified active components from this study and
further related studies, we can develop a new optimized formula. Specific Aim
1: To identify the active compounds responsible for the induction of HO-1.
Identification of the active components plays a key role in translating the
experience-based TCM to modern evidence-based medicine. The decoction ISF-1
is the popular formula for the therapy of ischemia reperfusion injury.
Intriguingly, most of the herbal ingredients are widely used to treat
inflammation and oxidative stress. The antioxidant activity appears to be
cytoprotective during reperfusion. However, both the active components and
the regulated pathways have not been well characterized. We have recently
demonstrated that the decoction ISF-1 can induce the high level expression of
HO |
|
Project Title: |
Integrative transcriptomic and proteomic analysis of the cellular responses of human liver cells to the bioactive saponin Astragaloside IV: Towards the mechanisms underlying the antioxidant activity of Chinese medicine Radix Astragali |
|
Investigator(s): |
Rong J |
|
Department: |
Medical Faculty |
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
03/2007 |
|
Abstract: |
|
Radix Astragali, known as Huang-Qi in traditional Chinese medicine, has been shown to possess a variety of biological and pharmacological activities such as anti-aging, anti-inflammation, and anti-oxidation in the central nerve system, cardiovascular system, and immune system [1-5]. Interestingly, we have recently demonstrated that the induction of heme oxygenase-1 (HO-1) expression is a key event to account for the antioxidant activity of traditional Chinese medicines. The induction of HO-1 enzyme was significantly enhanced by combination of Radix Astragali with other Chinese medicines such as Radix Chuangxiong and Lumbricus [6]. Furthermore, we have successfully demonstrated that astragaloside IV is the active compound responsible for regulating HO-1 expression. HO-1 is known to be cytoprotective against oxidative insults [7], suggesting that our results well pertain to the broad pharmacological activities of astragaloside IV, for example, inhibition of NF-kB activation [8] and suppression of excess accumulation of intracellular calcium [9]. Thus, we hypothesize that astragaloside IV is able to activate several intracellular pathways and consequently HO-1 is induced to a greater extent. The purpose of this study is to identify the cellular protein targets for drug discovery based on the genome-wide biological response profiles of human cells astragaloside IV and understand how astragaloside IV enhances the induction of HO-1 expression.Specific Aim # 1. To determine the differential gene expression profiles of human cells with/without the treatment of astragaloside IV. This specific aim is based on the hypothesis that astragaloside IV exhibit its activities through regulating cellular gene expression. Under this specific aim, we will perform a genome-wide analysis of the cellular gene expression modulated by astragaloside IV. The alteration of gene expression pattern should suggest the cellular protein targets for astragloside IV. In the future studies (beyond this proposal), the mRNA expression of candidate genes will be verified by quantitative RT-PCR. Protein expression will be quantified by Western blotting and its cellular localization will be determined by immunohistochemical staining. The results from this study will provide evidence for further characterization of the cellular proteins targeted by astragaloside IV.Specific Aim # 2. To determine the differential proteomic profiles of human cells with/without the exposure to astragaloside IV. This specific aim is based on the hypothesis that astragaloside IV may regulate post-transcriptional modifications of proteins. Under this specific aim, we will apply the proteomic approach to determine how astragaloside IV affects the cellular proteomic profiles by 2D gel electrophoresis and protein identification. Protein expression will be analyzed by western blotting (for quantification) and immunohistochemistry (for localization). The results of proteomic analysis will clarify whether astragaloside IV alters the post-transcriptional modification spectrum of the cellular proteins or not.Specific Aim # 3. To elucidate the role of astragaloside IV in the regulation of HO-1 expression. Our hypothesis is that astragaloside IV regulates HO-1 expression by activating a specific intracellular pathway. Based on the transcriptomic and proteomic profiles obtained in Specific Aim #1 & 2, we will first use specific inhibitors to determine which intracellular pathway is involved in regulating HO-1 expression. Candidate pathways may include NF-kB, nitric oxide production or calcium homeostasis. A promoter-reporter system, in which the reporter luciferase is under the control of 4.9 kb upstream DNA sequence of HO-1 gene, will be used to facilitate the detection of HO-1 expression.This project represents our new attempts to apply the state-of the-art genomics and proteomics technologies to characterize the biological response fingerprints (BioReF) of Chinese traditional medicines in human cells. The results from the proposed studies should be informative as to what types of biological pathways are targeted by the purified active component astragaloside IV. This knowledge will advance current understanding of the molecular changes that characterized the pharmacological activities of astragaloside IV and the molecular basis for its role in regulating HO-1 expression. Ultimately, this study will provide direct evidence to make use of the biological activities of astragaloside IV, especially enhancement of HO-1 induction, for therapeutic purpose. |
|
List of Research Outputs |
|
Lee D.C.W., Chik S.C.C., Li C.B., Yang L.H., Rong J., Tam P.K.H. and Lau A.S.Y., Application of Platform Technologies to Investigate the Immunomodulatory Effects of Ginseng, 14th Hong Kong International Cancer Congress, Hong Kong, 14-16 November 2007. |
|
Rong J., Tilton R., Shen J., Ng K.M., Liu C., Tam P.K.H., Lau A.S.Y. and Cheng Y.C., Genome-wide Biological Response Fingerprinting (BioReF) of the Chinese Botanical Formulatrion ISF-1 Enables the Selection of Multiple Marker Genes as a Potential Metric for Quality Control , Journal of Ethno-Pharmacology . 2007, 113: 35-44. |
|
Rong J., Cheung Y.H., Lau A.S.Y., Shen J., Tam P.K.H. and Cheng Y.C., Induction of heme oxygenase-1 by traditional Chinese medicine formulation ISF-1 and its ingredients as a cytoprotective mechanism against oxidative stress, Int J Mol Med. Athens, Greece, D.A. Spandidos, 2008, 21: 405-11. |
|
Rong J., Cheung C.Y.H., Lau A.S.Y., Shen J., Tam P.K.H. and Cheng Y.C., Induction of heme oxygenase-1 by traditional Chinese medicine formulation ISF-1 and its ingredients as a cytoprotective mechanism against oxidative stress, International Journal of Molecular Medicine. 2008, 21(4): 405-411. |
|
Researcher
: Shen F |
|
List of Research Outputs |
|
Shen F., 中藥6類新藥丹仙康骨膠囊研製 獲貴州省科學技術進步三等獎 (課題主持人), 2007. |
|
Shen F., 龐鼎元系列中醫專題講座 - 股骨頭缺血壞死的中醫藥治療空間的探討, 香港大學中醫藥學院, 2007. |
|
Shen F., 腰椎間盤突出症的診斷與中醫治療, 蘭靈閣圖書國際有限公司與香港中醫骨傷科學會, 2007. |
|
Shen F., 中西醫綜合病例討論 -- 肌骨病, 香港醫院管理局, 2007. |
|
Researcher
: Shen J |
|
Project Title: |
Effects of Buyang Huanwu Decoction on neuronal regeneration after ischemic brain injury |
|
Investigator(s): |
Shen J, Fung PCW |
|
Department: |
Medicine |
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
02/2004 |
|
Abstract: |
|
To evaluate the effects of Buyang Huanwu Decoction, a classical formula of Traditional Chinese Medicine (TCM), and its major component Astragalus membranaceus on the regeneration of neurons after ischemic brain injury. |
|
List of Research Outputs |
|
Liu B.Y. and Shen J., Effects of Buyang Huanwu Decoction on caveolin-1 and 2 of cerebral ischemia in rats, Journal of Hunan TCM University. 2008, 28(1): 22-28. |
|
Ren J., Fung P.C.W., Chang C., Shen G.G., Chan F.H.Y., Liu K.J. and Shen J., A comparative ESR study on blood and tissue nitric oxide concentration during renal ischemia-reperfusion injury , Applied Magnetic Resonance. Springer-Verlag, 2007, 32: 243-255. |
|
Rong J., Tilton R., Shen J., Ng K.M., Liu C., Tam P.K.H., Lau A.S.Y. and Cheng Y.C., Genome-wide Biological Response Fingerprinting (BioReF) of the Chinese Botanical Formulatrion ISF-1 Enables the Selection of Multiple Marker Genes as a Potential Metric for Quality Control , Journal of Ethno-Pharmacology . 2007, 113: 35-44. |
|
Rong
J., Cheung Y.H., Lau A.S.Y., Shen J., Cheng Y.C. and Tam P.K.H., Induction of heme
oxygenase-1 by the Chinese herbal formulation ISF-1 and its ingredients as a
cytoprotective mechanism against oxidative stress, The Sixth Meeting of
Consortium for Globalization of Chinese Medicine (CGCM). |
|
Rong J., Cheung Y.H., Lau A.S.Y., Shen J., Tam P.K.H. and Cheng Y.C., Induction of heme oxygenase-1 by traditional Chinese medicine formulation ISF-1 and its ingredients as a cytoprotective mechanism against oxidative stress, Int J Mol Med. Athens, Greece, D.A. Spandidos, 2008, 21: 405-11. |
|
Rong J., Cheung C.Y.H., Lau A.S.Y., Shen J., Tam P.K.H. and Cheng Y.C., Induction of heme oxygenase-1 by traditional Chinese medicine formulation ISF-1 and its ingredients as a cytoprotective mechanism against oxidative stress, International Journal of Molecular Medicine. 2008, 21(4): 405-411. |
|
Shen
J., Integration of Multiple Comprehensive
Approaches for Exploration of Therapeutic Principle of BHD |
|
Shen
J., Rong
J., So K.F., Lau A.S.Y. and Tam P.K.H., Integration of multiple
comprehensive approaches for exploration of therapeutic principle of BHD |
|
Shen
J., Rong
J., Tong Y., So K.F., Lau A.S.Y., Liu K.J., Tam P.K.H. and Cheng Y.C., Integration
of systemic biology, chemical profile fingerprint and functional imaging
technology for understanding therapeutic principle of Chinese medicinal
formula: a study on classical Post-Stroke Formula, The Sixth Meeting of
Consortium for Globalization of Chinese Medicine (CGCM). |
|
Shen
J., Reagents for highly specific detection of
peroxynitrite. , Patent: |
|
Shi J., Tong Y., Shen J. and Li H., Effectiveness and safety of herbal medicines in the treatment of irritable bowel syndrome: A systematic review., World Journal of Gastroenterology. 2008, Iss. 14(3): 454-462. |
|
Zeng H.P., Wang T.T., Chen W., Wang C.Y., Chen D.F. and Shen J., Characterization of chemical components in extracts from Si-wu decoction with proliferation-promoting effects on rat mesenchymal stem cells., Bioorg Med Chem. . 2008, 16(9): 5109-5114. |
|
Researcher
: Shi J |
|
List of Research Outputs |
|
Shi J., Tong Y., Shen J. and Li H., Effectiveness and safety of herbal medicines in the treatment of irritable bowel syndrome: A systematic review., World Journal of Gastroenterology. 2008, Iss. 14(3): 454-462. |
|
Researcher
: Song J |
|
List of Research Outputs |
|
Sze C.W., Song J., Chang R.C.C., Wong R.N.S., Tong Y. and Zhang Y., Research Advances on the Anti-aging Profile of Fructus lycii: an Ancient Chinese Herbal Medicine, Journal of Complementary and Integrative Medicine. 2008, Vol. 5: Iss. 1, Art. 8. |
|
Researcher
: Sze CW |
|
Project Title: |
Mechanistics Study on the Molecular Actions of a Modern Chinese Medicine Decoction (GFP) in Estrogen Secretion in Rat Granulose Cell via Aromatase Activation in vitro. |
|
Investigator(s): |
Sze CW, Tong Y, Zhang Y |
|
Department: |
|
|
Source(s) of Funding: |
Small Project Funding |
|
Start Date: |
12/2006 |
|
Abstract: |
|
Objective:According to a review of Women's Health Initiative (WHI) and Data and Safety Monitoring Board (DSMB) in 2004, there were more than 477 million peri-menopausal women in the world, and is expected to rise to 1.1 billion after 20 years .GFP decoction has been widely used for more than 50 years in Chinese Medicine relieving peri-menopausal syndrome. Peri-menopause is the period of gradual degeneration of ovarian function, which in turn lowers the estrogen level. However, GFP can elevate the estrogen level of estrogenic depletion patients undergoing peri-menopause back to their normal level.Based on the fact that the main role of granulose cells is estrogen secretion and the results of our clinical study showed that the estrogen level of peri-menopausal patients increases after treated by GFP, we are now intending to find out how GFP can stimulate the estrogen secretion in ovarian granulose cells in vitro.Hypothesis: GFP treatment results in increased estrogen level. This increased secretion of estrogen is attributed to the activation of aromatase in ovarian granulose cells. Aromatase is an enzyme that produces estrogen in granulose cells.The purpose of this study: To find out the action mechanism of GFP-induced estrogen secretion in rat granulose cell via aromatase activation in vitro. If the Hypothesis can be verified, the study should shed novel light on the relationship between GFP and aromatase activation. This will benefit the therapeutic approach to peri-menopausal syndromes.Key Issues of this study:Special Aim 1: To determine and compare the compounds in GFP by High Performance Liquid Chromatograph (HPLC) which can establish the chemical fingerprint maps of GFP for quality control purpose.Special Aim 2: To establish a suitable cell model for studying the molecular event regulating granulosa cell steroidogenesis by GFP. Special Aim 3: To test the implication of the hypothesis, i.e. GFP-induced estrogen secretion in granulosa cells via aromatase activation, and to find out the brief action mechanism of GFP-induced estrogen secretion in rat granulose cells via aromatase activation in vitro. |
|
List of Research Outputs |
|
Chen
H., Cho W.C.S., Sze C.W.
and Tong Y., A Systematic Review on
Efficacy of Erxian Decoction Treated Menopausal Symptoms, 3rd
International Congress Complementary Medicine Research. |
|
Chen H., Cho W.C.S., Sze C.W. and Tong Y., Treatment of Menopausal Symptoms with Er-xian Decoction: A Systematic Review, America Journal of Chinese Medicine. 2008, 36(2): 233-244. |
|
|
|
Li H.,
Sze C.W. and Tong Y., Immunomodulation Induced By
Chinese Medicine Herb, Rhodiola algida, On Th1- and Th2-dependent Cytokine
Production., 3rd International Congress Complementary Medicine Research
(Poster Abstract, p118). |
|
Liu Q., Loo W.T.Y., Sze C.W., Tong Y. and Chow L.W.C., Curcumin’s effect on breast cancer cell – a preclinical trial, The 6th Biennial Meeting of the Asian Breast Cancer Society (ABCS). 2007, 20-22. |
|
Sze C.W., Zhang Y., Shaw P.C., But P.P.H., Ng T.B. and Tong Y., A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region, Biotechnology and Applied Biochemistry. 2008, 49: 149-154. |
|
Sze
C.W., Chinese Medicine for the
peri-menopausal sydrome: theory and experiment, Department of Health
Technology & Informatics, the |
|
Sze
C.W., Zhang
Y., Lau A.S.Y. and Tong Y., Developing method to screen for
endocrine-active Chinese medicine formulation to relieve the menopausal
syndrome, Sixth Meeting of Consortium for Globalization of Chinese
Medicine (Poster). |
|
Sze
C.W., Zhang
Y., Lau A.S.Y. and Tong Y., Erxian Decoction Stimulates
Estrogen Secretion Via Steroidogenic Signaling Transduction Pathway In Vivo.,
3rd International Congress Complementary Medicine Research . |
|
Sze C.W., Zhang Y., Zhang Z., Lau A.S.Y. and Tong Y., Erxian decoction stimulates estrogen secretion via steroidogenic signalling transduction pathway in vivo, 3rd International Congress on Complementary Medicine Research, Sydney, Australia, 29-31 March 2008 (Poster Abstract, p120). 2008, 60. |
|
Sze C.W., Lau A.S.Y., Zhang Y., Tong Y. and Zhang Z., Mechanical Study of Erxian Decoction for Peri-menopausal Syndrome., Third International Symposium on Healthy Aging: Improving the Health of an Aging Population. (Speaker). 2008. |
|
Sze C.W., Song J., Chang R.C.C., Wong R.N.S., Tong Y. and Zhang Y., Research Advances on the Anti-aging Profile of Fructus lycii: an Ancient Chinese Herbal Medicine, Journal of Complementary and Integrative Medicine. 2008, Vol. 5: Iss. 1, Art. 8. |
|
Sze C.W., Zhang Z. and Tong Y., Traditional Chinese Medicine for Parkinson’s Disease: Basic Theory and Preclinical Screening., Croucher Advanced Study Institute: Innovative Therapies of Movement Disorders: Basic and Clinical Sciences.. 2007. |
|
Sze C.W., Traditional Chinese Medicine for Parkinson’s Disease: Basic Theory and Preclinical Screening, Croucher Advanced Study Institute: Innovative Therapies of Movement Disorders: Basic and Clinical Sciences.. 2007. |
|
Tong
Y., Sze C.W., Zhang Y., Zhang Z. and Lau A.S.Y., "Mechanical Study of
Erxian Decoction for Peri-menopausal Syndrom”, Third International Healthy
Aging - Improving the Health of an Aging Population”, |
|
Yuan H.N., Wang C.Y., Sze C.W., Tong Y., Tan Q.R., Feng X.J., Jiu R.M., Zhang J.Z., Zhang Y. and Zhang Z., A Randomized Crossover Comparison of Herbal Medicine and Bromocriptine against Risperidone-Induced Hyperprolactinemia in Patients with Schizophrenia, Journal of Clinical Psychopharmacology. 2008, 28: 264-370. |
|
Zhang Y., Tong Y., Sze C.W., Feng Y., Song J.X., Wong R.N.S. and NG T.B., Application of sequence characterized amplified region (SCAR) analysis to authenticate Lycium barbarum and its adulterants., In: Stephen Sze CW, Song JX, Ricky Wong NS, Ng TB, Tong Y, Kalin Zhang YB. , Biotechnology and Applied Biochemistry . 2007, 51: 15-21. |
|
Zhang Y., Tong Y. and Sze C.W., PI: HK$200,000 (2007).Donation from the Li Ka Shing Foundation : Matching Grant for Seed Funding Programme. Donation from LKS Faculty of Medicine.No. 20460016. , In: Zhang YB, Sze CW, Tong Y. , LKS Faculty of Medicine. 2007. |
|
Researcher
: Tang J |
|
List of Research Outputs |
|
Tang
J., Feng
Y., Tong Y., Jia R., Sy L.K. and Man R.Y.K., The Mucilage Cavity is a
Distinct Microscopic Characteristic Showing in the Phloem but not Pith of
Rhizomes for the Identification of Radix et Rhizoma Rhei., In: Editoral
Department of Wuhan University Journal of Natural Sciences, Wuhan
University Journal of Natural Sciences. |
|
Researcher
: Tong Y |
|
Project Title: |
Development of Sequence Characterized Amplified Region (SCAR) Markers to Differentiate Fengdou Shihu (枫斗石斛) and Its Adulterants. |
|
Investigator(s): |
Tong Y, Zhang Y, Sze CW |
|
Department: |
|
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
03/2006 |
|
Abstract: |
|
Fengdou Shihu (FDSH) from Dendrobium
Officinale (铁皮石斛) (Family: orchidaceae) is a valuable
and tonic Chinese medicine, which have gained considerable favor in |
|
Project Title: |
To evaluate the anti-invasion, anti-metastasis, multidrug resistance property and chemoprevention effect of curcumin for the treatment of breast cancer. |
|
Investigator(s): |
Tong Y, Sze CW |
|
Department: |
|
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
04/2007 |
|
Abstract: |
|
The purpose of this study is to evaluate
the anti-invasion, anti-metastasis, multidrug resistance property and
chemoprevention effect of curcumin for the treatment of breast cancer.Breast
cancer, is the most common cancer among women in |
|
List of Research Outputs |
|
Chen
H., Cho W.C.S., Sze C.W. and Tong Y., A Systematic Review on
Efficacy of Erxian Decoction Treated Menopausal Symptoms, 3rd
International Congress Complementary Medicine Research. |
|
Chen H., Cho W.C.S., Sze C.W. and Tong Y., Treatment of Menopausal Symptoms with Er-xian Decoction: A Systematic Review, America Journal of Chinese Medicine. 2008, 36(2): 233-244. |
|
|
|
Feng Y., Tsao G.S.W., Tong Y. and Ng K.M., Matching Grant (4th Phase)-Research project on alternative drug for bear bile, UGC (Government Matching Grant Scheme). 2008, HK$402,500. |
|
Feng Y., Tsao G.S.W., Tong Y. and Ng K.M., Research project on alternative drug for bear bile, Yiqingzhai Foundation (The Pong Ding Yueng Endowment Fund for Education & Research in Chinese-Western Medicine). 2008, HK$805,000. |
|
Feng Y. and Tong Y., 从中药现代研究看中医学的基本特点, 香港中医杂志. Hong Kong, 香港中医杂志出版部, 2008, 3(1): 17-21. |
|
Feng Y., Ye X. and Tong Y., 建立香港中藥不良反應監測系統和發揮中醫藥在香港藥物濫用防治中的作用, 中國藥物濫用防治雜誌. Beijing., Publisher of Chinese Journal of Drug Abuse Prevention and Tr, 2007, 13(4): 220-223. |
|
Li H.,
Sze C.W. and Tong Y., Immunomodulation Induced By
Chinese Medicine Herb, Rhodiola algida, On Th1- and Th2-dependent Cytokine
Production., 3rd International Congress Complementary Medicine Research
(Poster Abstract, p118). |
|
Liu Q., Loo W.T.Y., Sze C.W., Tong Y. and Chow L.W.C., Curcumin’s effect on breast cancer cell – a preclinical trial, The 6th Biennial Meeting of the Asian Breast Cancer Society (ABCS). 2007, 20-22. |
|
Luk J.M.C., Wang X., Liu P., Wong K.F., Chan K.L., Tong Y., Hui C.K., Lau G. and Fan S.T., Traditional Chinese herbal medicines for treatment of liver fibrosis and cancer: from laboratory discovery to clinical evaluation, Liver International. 2007, 27(7): 879-890. |
|
Shen
J., Rong J., Tong Y., So K.F., Lau A.S.Y., Liu K.J., Tam P.K.H. and Cheng Y.C., Integration
of systemic biology, chemical profile fingerprint and functional imaging
technology for understanding therapeutic principle of Chinese medicinal
formula: a study on classical Post-Stroke Formula, The Sixth Meeting of
Consortium for Globalization of Chinese Medicine (CGCM). |
|
Shi J., Tong Y., Shen J. and Li H., Effectiveness and safety of herbal medicines in the treatment of irritable bowel syndrome: A systematic review., World Journal of Gastroenterology. 2008, Iss. 14(3): 454-462. |
|
Sze C.W., Zhang Y., Shaw P.C., But P.P.H., Ng T.B. and Tong Y., A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region, Biotechnology and Applied Biochemistry. 2008, 49: 149-154. |
|
Sze
C.W., Zhang Y., Lau A.S.Y. and Tong Y., Developing method to screen
for endocrine-active Chinese medicine formulation to relieve the menopausal
syndrome, Sixth Meeting of Consortium for Globalization of Chinese
Medicine (Poster). |
|
Sze
C.W., Zhang Y., Lau A.S.Y. and Tong Y., Erxian Decoction Stimulates
Estrogen Secretion Via Steroidogenic Signaling Transduction Pathway In Vivo.,
3rd International Congress Complementary Medicine Research . |
|
Sze C.W., Zhang Y., Zhang Z., Lau A.S.Y. and Tong Y., Erxian decoction stimulates estrogen secretion via steroidogenic signalling transduction pathway in vivo, 3rd International Congress on Complementary Medicine Research, Sydney, Australia, 29-31 March 2008 (Poster Abstract, p120). 2008, 60. |
|
Sze C.W., Lau A.S.Y., Zhang Y., Tong Y. and Zhang Z., Mechanical Study of Erxian Decoction for Peri-menopausal Syndrome., Third International Symposium on Healthy Aging: Improving the Health of an Aging Population. (Speaker). 2008. |
|
Sze C.W., Song J., Chang R.C.C., Wong R.N.S., Tong Y. and Zhang Y., Research Advances on the Anti-aging Profile of Fructus lycii: an Ancient Chinese Herbal Medicine, Journal of Complementary and Integrative Medicine. 2008, Vol. 5: Iss. 1, Art. 8. |
|
Sze C.W., Zhang Z. and Tong Y., Traditional Chinese Medicine for Parkinson’s Disease: Basic Theory and Preclinical Screening., Croucher Advanced Study Institute: Innovative Therapies of Movement Disorders: Basic and Clinical Sciences.. 2007. |
|
Tang
J., Feng Y., Tong Y., Jia R., Sy L.K. and Man R.Y.K., The Mucilage Cavity is a
Distinct Microscopic Characteristic Showing in the Phloem but not Pith of
Rhizomes for the Identification of Radix et Rhizoma Rhei., In: Editoral
Department of Wuhan University Journal of Natural Sciences, Wuhan
University Journal of Natural Sciences. |
|
Tong
Y., Sze
C.W., Zhang Y., Zhang Z. and Lau A.S.Y., "Mechanical Study of
Erxian Decoction for Peri-menopausal Syndrom”, Third International Healthy
Aging - Improving the Health of an Aging Population”, |
|
Tong Y., "Physical condition and healthcare”, HKSAR Civil Servant Training and Development Institute (CSTDI). 2007. |
|
Tong
Y., "The Mechanical Study of EXD for
Peri-menopausal Syndrom”, Department of Pharmacology, |
|
Tong Y., "The development of research of Prevention from oncology with Chinese Medicine” at its Press Conference for educational books for cancer prevention “Integration medicine-Love makes miracles” (癌症防治科普讀物 "中西合璧, 愛顯奇跡"), China Resources Retail (Group) Co., Ltd.. 2008. |
|
Tong
Y., "The roles of the Universities in
the training of TCM professionals in Hong Kong" under main theme session
“Training and Professional Development of Chinese Medicine practitioners”, 2007
TWGHs Eddie Wang Symposium on Integrated Chinese and Western Medicine, Hong
Kong (speaker). |
|
Tong Y., "The roles of the Universities in the training of TCM professionals in Hong Kong” under main theme session “Training and Professional Development of Chinese Medicine practitioners”, 2007 TWGHs Eddie Wang Symposium on Integrated Chinese and Western Medicine, Hong Kong. 2007. |
|
Tong Y., Comparison of the liver system between Chinese medicine "Gan" and Western medicine "liver", Hong Kong - Shanghai International Liver Congress (Speaker). 2008. |
|
Tong Y., Comparison of the liver system between Chinese medicine”Gan” and western Medicine “liver”, Hongkong – Shanghai International Liver Congress (Speaker). 2008. |
|
Tong Y., Mechanical Study of Erxian Decoction for Peri-menopausal Syndrome, HKU TCM Workshop. 2007. |
|
Tong
Y., Zhang N.X. and Liu Q., Study on the Action
Mechanism of "ShuGan LiFei" Therapy on Experimental Rats with
Asthma and under Stress, World Conference of Stress, |
|
Tong Y., to visit the pharmacology college and brain research center and gave a presentstion” the research status of school of Chinese Medicine of HKU”, The University of New Mexico. 2008. |
|
Tong Y., "Chinese medicine for the good health of the Elderly” 創新老年痴呆症活動工作坊2008, 醫院管理局職業治療統籌委員會, 香港老年痴呆症協會, 2008. |
|
Tong Y., 中醫的觀念II, University Health Service, HKU, 2008. |
|
Tong Y., 中醫的觀念 I, University Health Service, HKU, 2008. |
|
Wang D.N., Lin S.H., Yang D.P., Jin J., Tong Y. and Chen J., Characterization of steroidal
saponins in crude extract from Dioscorea Nipponica Makino by liquid
chromatography tandem multi-stage mass spectrometry, Analytica Chimica
Acta. |
|
Ye X.,
Feng Y., Tong Y. and Tsao G.S.W., The effects of coptis
extract on hepatoprotection in rats with liver damage., 2007 Hong
Kong-Macau postgraduate Symposium on Chinese Medicine. |
|
Yuan H.N., Wang C.Y., Sze C.W., Tong Y., Tan Q.R., Feng X.J., Jiu R.M., Zhang J.Z., Zhang Y. and Zhang Z., A Randomized Crossover Comparison of Herbal Medicine and Bromocriptine against Risperidone-Induced Hyperprolactinemia in Patients with Schizophrenia, Journal of Clinical Psychopharmacology. 2008, 28: 264-370. |
|
Zhang Y., Tong Y., Sze C.W., Feng Y., Song J.X., Wong R.N.S. and NG T.B., Application of sequence characterized amplified region (SCAR) analysis to authenticate Lycium barbarum and its adulterants., In: Stephen Sze CW, Song JX, Ricky Wong NS, Ng TB, Tong Y, Kalin Zhang YB. , Biotechnology and Applied Biochemistry . 2007, 51: 15-21. |
|
Zhang
Y. and Tong Y., Nature
Anti-cancer Medicine- Ganoderma and cordyceps. , In: Xie Yong Zheng (Ed)., lovingness
ferly- integrate of Chinese Medicine and western medicine.. |
|
Zhang Y., Tong Y. and Sze C.W., PI: HK$200,000 (2007).Donation from the Li Ka Shing Foundation : Matching Grant for Seed Funding Programme. Donation from LKS Faculty of Medicine.No. 20460016. , In: Zhang YB, Sze CW, Tong Y. , LKS Faculty of Medicine. 2007. |
|
Zhang Z., Tan Q.R., Tong Y., Li Q., Kang W.H., Zhen X.C. and Post R.M., The effectiveness of carbamazepine in unipolar depression: a double-blind, randomized, placebo-controlled study. , Journal of Affective Disorders. 2008, 109: 91-97. |
|
Researcher
: Wang TT |
|
List of Research Outputs |
|
Zeng H.P., Wang T.T., Chen W., Wang C.Y., Chen D.F. and Shen J., Characterization of chemical components in extracts from Si-wu decoction with proliferation-promoting effects on rat mesenchymal stem cells., Bioorg Med Chem. . 2008, 16(9): 5109-5114. |
|
Researcher
: Wu J |
|
Project Title: |
Anti-cystic hyperplasia of breast herbal medicine research |
|
Investigator(s): |
Wu J, Chow LWC |
|
Department: |
|
|
Source(s) of Funding: |
Other Funding Scheme |
|
Start Date: |
05/2001 |
|
Abstract: |
|
To study anti-cystic hyperplasia of breast herbal medicine. |
|
Project Title: |
Anti-prostatomegaly herbal medicine research |
|
Investigator(s): |
Wu J, Leung SYL |
|
Department: |
|
|
Source(s) of Funding: |
Other Funding Scheme |
|
Start Date: |
06/2001 |
|
Abstract: |
|
To study the effectiveness of anti-prostatomegaly herbal medicine. |
|
Researcher
: Ye X |
|
List of Research Outputs |
|
Feng Y., Ye X. and Tong Y., 建立香港中藥不良反應監測系統和發揮中醫藥在香港藥物濫用防治中的作用, 中國藥物濫用防治雜誌. Beijing., Publisher of Chinese Journal of Drug Abuse Prevention and Tr, 2007, 13(4): 220-223. |
|
Ye
X., Feng
Y., Tong Y. and Tsao G.S.W., The effects of coptis
extract on hepatoprotection in rats with liver damage., 2007 Hong
Kong-Macau postgraduate Symposium on Chinese Medicine. |
|
Researcher
: Yiu YM |
|
Project Title: |
The clinical effectiveness of acupuncture in the treatment of chronic fatigue syndrome (CFS) |
|
Investigator(s): |
Yiu YM, Ng SM |
|
Department: |
|
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
01/2006 |
|
Abstract: |
|
The main objective of this research is to conduct a randomized controlled clinical trial to evaluate the effectiveness of acupuncture on CFS |
|
List of Research Outputs |
|
Ng
S.M., Tsui E.Y.L. and Yiu Y.M., Acupuncture and chronic
fatigue syndrome: A Randomized controlled trial, World Mental Health
Congress. |
|
Tsui
E.Y.L., Ng S.M. and Yiu Y.M., Co-occurrence of irritable
bowel symptoms and chronic fatigue syndrome: Prevalence in |
|
Yiu Y.M., Ng S.M., Tsui E.Y.L. and Chan Y.L., A clinical trial of acupuncture for chronic fatigue syndrome, 針刺治療慢性疲勞綜合征的臨床研究, Hong Kong Chinese Medical Journal. 香港中醫雜誌, Hong Kong, 2008, 3(1): 76-79. |
|
Yiu Y.M., Ng S.M., Tsui E.Y.L. and Chan Y.L., A clinical trial of acupuncture for treating chronic fatigue syndrome in Hong Kong, Journal of Chinese Integrative Medicine (Zhong Xi Yi Jie He Xue Bao). 2007, 5: 630-633. |
|
Yiu
Y.M., Tsui
E.Y.L. and Ng S.M., The seasonal
effect of acupuncture in the treatment of chronic fatigue syndrome, World Mental
Health Congress. |
|
Researcher
: Zhang Y |
|
Project Title: |
Comparison of Pharmacological Effects and Chemical Constituents between Different Dendrobium Species |
|
Investigator(s): |
Zhang Y, Tong Y, Sze CW |
|
Department: |
|
|
Source(s) of Funding: |
Small Project Funding |
|
Start Date: |
01/2007 |
|
Abstract: |
|
Objectives:The Chinese crude drug
"Shihu", which is derived from the dried or fresh stems of
Dendrobium species (Orchidaceae), is a valuable and tonic Chinese medicine
and have gained considerable favor in |
|
List of Research Outputs |
|
Sze C.W., Zhang Y., Shaw P.C., But P.P.H., Ng T.B. and Tong Y., A DNA microarray for differentiation of the Chinese medicinal herb Dendrobium officinale (Fengdou Shihu) by its 5 S ribosomal DNA intergenic spacer region, Biotechnology and Applied Biochemistry. 2008, 49: 149-154. |
|
Sze
C.W., Zhang Y., Lau A.S.Y. and Tong Y., Developing method to screen for
endocrine-active Chinese medicine formulation to relieve the menopausal
syndrome, Sixth Meeting of Consortium for Globalization of Chinese
Medicine (Poster). |
|
Sze
C.W., Zhang Y., Lau A.S.Y. and Tong Y., Erxian Decoction Stimulates
Estrogen Secretion Via Steroidogenic Signaling Transduction Pathway In Vivo.,
3rd International Congress Complementary Medicine Research . |
|
Sze C.W., Zhang Y., Zhang Z., Lau A.S.Y. and Tong Y., Erxian decoction stimulates estrogen secretion via steroidogenic signalling transduction pathway in vivo, 3rd International Congress on Complementary Medicine Research, Sydney, Australia, 29-31 March 2008 (Poster Abstract, p120). 2008, 60. |
|
Sze C.W., Lau A.S.Y., Zhang Y., Tong Y. and Zhang Z., Mechanical Study of Erxian Decoction for Peri-menopausal Syndrome., Third International Symposium on Healthy Aging: Improving the Health of an Aging Population. (Speaker). 2008. |
|
Sze C.W., Song J., Chang R.C.C., Wong R.N.S., Tong Y. and Zhang Y., Research Advances on the Anti-aging Profile of Fructus lycii: an Ancient Chinese Herbal Medicine, Journal of Complementary and Integrative Medicine. 2008, Vol. 5: Iss. 1, Art. 8. |
|
Tong Y., Sze C.W., Zhang Y., Zhang Z. and Lau A.S.Y., "Mechanical Study of Erxian Decoction for Peri-menopausal Syndrom”, Third International Healthy Aging - Improving the Health of an Aging Population”, Hong Kong (Speaker). 2008. |
|
Yuan H.N., Wang C.Y., Sze C.W., Tong Y., Tan Q.R., Feng X.J., Jiu R.M., Zhang J.Z., Zhang Y. and Zhang Z., A Randomized Crossover Comparison of Herbal Medicine and Bromocriptine against Risperidone-Induced Hyperprolactinemia in Patients with Schizophrenia, Journal of Clinical Psychopharmacology. 2008, 28: 264-370. |
|
Zhang Y., Anti-Osteoporosis Activity of Naringin in the Retinoic Acid-Induced Osteoporosis Model , In: Min Wei, Ping Li, Kalin Yanbo Zhang, Zhonglin Yang, American Journal of Chinese Medicine . 2007. |
|
Zhang Y., Tong Y., Sze C.W., Feng Y., Song J.X., Wong R.N.S. and NG T.B., Application of sequence characterized amplified region (SCAR) analysis to authenticate Lycium barbarum and its adulterants., In: Stephen Sze CW, Song JX, Ricky Wong NS, Ng TB, Tong Y, Kalin Zhang YB. , Biotechnology and Applied Biochemistry . 2007, 51: 15-21. |
|
Zhang Y., Authentication of Lycium barbarum and its adulterants by sequence characterized amplified region (SCAR) analysis., In: Zhang Yanbo, Tong Yao, Sze Cho Wing, 3rd International Congress on Complementary Medicine Research. . 2008. |
|
Zhang Y., Chemical and Molecular Identification of Hong Dangshen, A Unique Medicinal Material for Diarrhea in Hong Kong , In: Zhang Yan-Bo, Jiang Ren-Wang, Li Song-Lin, Qiao Chun-Feng, Han Quan-Bin, Xu Hong-Xi, Wong Ka-Lok, But Paul Pui-Hay, Shaw Pang-Chui, Chinese Journal of Pharmaceutical Science . 2007. |
|
Zhang Y., Council member in Journal of Pharmaceutical and Biological Technology., In: Zhangyanbo, Journal of Pharmaceutical and Biological Technology. 2007. |
|
Zhang
Y., DNA microarray for identification of
Chinese Medicine., In: Zhang Yanbo, Chinese Medicine Serial Active of |
|
Zhang
Y., Development of DNA Microarray for High
Throughput Identification of Chinese Medicinal Formulations., In: Zhang
Yanbo, Sixth Meeting of Consortium for Globalization of Chinese Medicine
(CGCM).. |
|
Zhang Y., Molecular Authentication of Chinese Medicines, In: Zhang YB, International Conference & Exhibition of the Modernization of Chinese Medicine & Health Products. 2007. |
|
Zhang
Y. and Tong
Y., Nature Anti-cancer Medicine- Ganoderma and cordyceps. , In: Xie Yong
Zheng (Ed)., lovingness ferly- integrate of Chinese Medicine and western
medicine.. |
|
Zhang Y., PI: HK$100,000 (2007). Top-up fund for support the Postgraduate Studentships of MPhil or PhD students admitted. Funding from LKS Faculty of Medicine., In: Zhang YB , LKS Faculty of Medicine. 2007. |
|
Zhang Y., Tong Y. and Sze C.W., PI: HK$200,000 (2007).Donation from the Li Ka Shing Foundation : Matching Grant for Seed Funding Programme. Donation from LKS Faculty of Medicine.No. 20460016. , In: Zhang YB, Sze CW, Tong Y. , LKS Faculty of Medicine. 2007. |
|
Zhang
Y., PI: HK$50,000 (2008). Donation from the
Li Ka Shing Foundation : CERG Incentive Award. Project No 20460013. |
|
Zhang Y., Parmacology study in Blueberry Extract, In: Zhang Yanbo , Doctor health company . 2007. |
|
Zhang
Y., Special topic lecture: toxicity Chinese
Medicine-Bufo bufo gargarizans Canror., In: Zhang Yanbo, In the |
|
Zhang
Y., death case on toxicity Chinese
Medicine-Bufo bufo gargarizans Canror., In: Zhang Yanbo, In the law court
of Xi Wan Ho, |
|
Researcher
: Zhang Z |
|
Project Title: |
Therapeutic effects of ligustilide and mechanisms of its actions against premenstrual dysphoric disorder (PMDD) |
|
Investigator(s): |
Zhang Z |
|
Department: |
|
|
Source(s) of Funding: |
Seed Funding Programme for Basic Research |
|
Start Date: |
12/2006 |
|
Abstract: |
|
1. Objective The objective of the proposed project is to determine therapeutic effects of ligustilide, an essential oil initially derived from the Chinese medicine Angelica sinensis (Dang-Gui), and mechanisms of its actions against premenstrual dysphoric disorder (PMDD). PMDD symptoms mainly include severe depressed mood, anxiety, anger, and increased interpersonal conflicts. Although the antidepressants selective serotonin reuptake inhibitors (SSRIs) have been first-line therapy of PMDD, over 50% of the patients cannot obtain clinically defined responses. The incompleteness of SSRI therapy is largely because the etiology and pathogenesis of PMDD are apparently different from major depressive disorders. Since decreased sensitivity of central gamma-aminobutyric acid (GABA) receptors induced by dramatic fluctuation in ovarian steroids during the cycle is the major pathogenesis of PMDD is. Therefore, it is reasonable to believe that agents that can restore GABA receptor sensitivity by stabilizing ovarian steroid fluctuation should yield better clinical efficacy than SSRIs in the treatment of PMDD. Angelica sinensis is the most commonly used Chinese medicine in treating menstrual cycle-linked mood disorders. Ligustilide is an essential oil which initially derived from Angelica and accounts for nearly 45% of total Angelica essential oils. Behavioral studies have shown that ligustilide possesses broad psychotropic effects, in particular, anxiolytic, social conflict-improved, and antidepressant effects; the effects are modulated by central GABAergic systems. Furthermore, ligustilide also possesses estrogenic and neurosteroid-like activities. These observations have led to the hypotheses that ligustilide may have specific therapeutic effects against PMDD by stabilizing ovarian steroid fluctuation during the cycle, and the effects are regulated by certain GABA and 5-HT receptor subtypes. To test these hypotheses, the following specific aims are proposed: 1) To evaluate the therapeutic effects of ligustilide against anxiety, depressive, and social conflict behavior observed in a PMDD model (female rats in the estrous phase); 2) To characterize GABAA and 5-HT2 receptor subtypes in the prefrontal cortex, hippocampus, and amygdala that modulate behavioral actions of ligustilide; and 3) To determine if ligustilide pretreatment could normalize dysfunction of GABAA receptor subtypes induced by withdrawal from exposure to the neurosteroid allopregnanolone in cultured neuronal cells. Through achieving these specific aims, we attempt to answer the following questions: (1) Does ligustilide have comparable even better effects in inhibiting PMDD-like behavior compared to reference drugs? (2) Are behavioral effects of ligustilide correlated with decreases in the amplitude of fluctuation in ovarian steroids? (3) Can GABAA and 5-HT2 receptor antagonists abolish behavioral effects of ligustilide observed in PMDD models? and (4) How does ligustilide treatment change the expression of GABAA receptor subtypes in specific brain regions and cultured neuronal cells? 2. Background The idea that comes from our previous studies: Jia-Wei-Xiao-Yao-San (Free and Easy Wanderer Plus, FEWP) is a well known Chinese herbal mixture which principal indications are menstrual cycle-associated mood symptoms, including premenstrual tension and menopausal syndrome. In previous studies, we have shown the beneficial effects of FEWP as adjunctive therapy and monotherapy in patients with mood disorders. In particular, FEWP monotherapy yields significantly greater improvement than placebo in patients with unipolar and bipolar depression, and this greater improvement appears to be more significant in female patients. These findings have triggered our interest to further explore potential constituents contained in FEWP that may have specific effects against menstrual cycle-linked mood disorders, such as PMDD. Since Angelica sinensis is a principal herb in the FEWP formula and most commonly used for menstrual cycle-linked illnesses, its major constituent ligustilide naturally came into our first consideration. What is known about ligustilide: Ligustilide is a low molecular compound (C12H14O2: MW: 190.24), easily penetrating into the blood-brain barrier. Ligustilide has been shown to have broad psychotropic effects. Acute administration of ligustilide significantly improves anxiety-like and depressive behavior in male rats. Ligustilide treated animals also display significant improvements on aggressive in social interaction test. Moreover, ligustilide reverses heightened social isolation induced by FG7142, a GABAA receptor blocker. However, these effects of ligustilide remain to be evaluated. Ligustilide has moderate to high affinity at estrogen receptors and GABA receptors. It also up-regulates progesterone receptor mRNA and enhances the release of 5-hydroxytryptamine (5-HT) and noradrenaline (NA), suggesting that ligustilide has both estrogenic and neurosteroid effects, including regulating GABA, NA and 5-HT activities. It is therefore reasonable to believe that ligustilide may have specific effects against the etiology and pathogenesis of PMDD. What is known about PMDD: Dramatic fluctuation in the ovarian hormone progesterone (P) and its metabolite allopregnanolone (ALLO) during the cycle is believed to be a major etiological factor of PMDD. Since ALLO is a potent positive modulator of GABAA receptor that enhances central GABAergic inhibition, high levels of ALLO during the cycle may induce excessive inhibition and low level or sharp drop may induce withdrawal effects, resulting in negative mood, anxiety, irritability, and social conflict in certain individuals. Furthermore, reduced sensitivity of GABAA receptor subtypes in specific brain regions was found to be associated with changes in ALLO levels, recurrence and severity of PMDD experienced during the cycle. These observations have led to a belief that restoration of GABAA receptors by stabilizing the steroid fluctuations is an effective strategy in the treatment of PMDD. On the other hand, it has been known that the 5-HT2 receptor plays an important role in regulating therapeutic effects of SSRIs in the treatment of PMDD. We will evaluate effects of ligustilide on the expression of 5-HT2 receptor subtypes in brain regions. Models of PMDD: The most widely used model is female rats in the estrus phase. Behavioral responses of estrous-phase animals in the elevated plus-maze (EPM), forced swimming test (FST), and social interaction (SI) test are nearly 70%-110% higher than diestrous-phase animals, which well resemble anxiety, depression, and social conflict observed in PMDD patients. We will utilize the three paradigms to test anti-PMDD effects of ligustilide. Cultured rat hippocampal neurons express abundant various GABAA receptor subtypes, and changes in the expression induced by withdrawal from the exposure to neurosteroids are consistent with the pathogenesis of PMDD. We will use the cultured cellsto detect molecular mechanisms of ligustilide’s actions. |
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List of Research Outputs |
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Sze C.W., Zhang Y., Zhang Z., Lau A.S.Y. and Tong Y., Erxian decoction stimulates estrogen secretion via steroidogenic signalling transduction pathway in vivo, 3rd International Congress on Complementary Medicine Research, Sydney, Australia, 29-31 March 2008 (Poster Abstract, p120). 2008, 60. |
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Sze C.W., Lau A.S.Y., Zhang Y., Tong Y. and Zhang Z., Mechanical Study of Erxian Decoction for Peri-menopausal Syndrome., Third International Symposium on Healthy Aging: Improving the Health of an Aging Population. (Speaker). 2008. |
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Sze C.W., Zhang Z. and Tong Y., Traditional Chinese Medicine for Parkinson’s Disease: Basic Theory and Preclinical Screening., Croucher Advanced Study Institute: Innovative Therapies of Movement Disorders: Basic and Clinical Sciences.. 2007. |
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Tong Y., Sze C.W., Zhang Y., Zhang Z. and Lau A.S.Y., "Mechanical Study of Erxian Decoction for Peri-menopausal Syndrom”, Third International Healthy Aging - Improving the Health of an Aging Population”, Hong Kong (Speaker). 2008. |
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Yu Y., Wang J.R., Sun P.H., Guo Y., Jin G.Z., Zhang Z. and Zhen X., Neuroprotective effects of atypical D1 receptor agonist SKF83959 are mediated via D1 receptor-dependent inhibition of glycogen synthase kinase-3 beta and a receptor-independent anti-oxidative action., Journal of Neurochemistry. 2008, 104: 946-956. |
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Yuan H.N., Wang C.Y., Sze C.W., Tong Y., Tan Q.R., Feng X.J., Jiu R.M., Zhang J.Z., Zhang Y. and Zhang Z., A Randomized Crossover Comparison of Herbal Medicine and Bromocriptine against Risperidone-Induced Hyperprolactinemia in Patients with Schizophrenia, Journal of Clinical Psychopharmacology. 2008, 28: 264-370. |
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Zhang Z., Chinese herbal medicine and acupuncture therapy of major psychiatric disorders , 2007 World Mental Health Congress of the World Federation for Mental Health, Hong Kong, August 21, 2007. |
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Zhang Z., Tan Q.R., Tong Y., Li Q., Kang W.H., Zhen X.C. and Post R.M., The effectiveness of carbamazepine in unipolar depression: a double-blind, randomized, placebo-controlled study. , Journal of Affective Disorders. 2008, 109: 91-97. |
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