DEPARTMENT OF BOTANY
| Researcher : Cai Y |
| Project Title: | Investigating structure-activity relationships of natural phenolic antioxidants from traditional Chinese medicinal plants |
| Investigator(s): | Cai Y, Sun M, Corke H |
| Department: | Botany |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To investigate and elucidate the structure-antioxidant activity relationships of representative natural phenolic antioxidants from traditional Chinese medicinal plants associated with anticancer using the improved ABTS⋅+ method (TEAC assay) at the basis of chemical structural analysis. |
| List of Research Outputs |
| Bao J., Cai Y., Sun M., Wang G.Y. and Corke H., Anthocyanins, Flavonols, and Free Radical Scavenging Activity of Chinese Bayberry (Myrica rubra) Extracts and Their Color Properties and Stability, Journal of Agricultural and Food Chemistry. 2005, 53: 2327-2332. |
| Cai Y., Sun M., Xing J. and Corke H., Antioxidant Phenolic Constituents in Roots of Rheum officinale and Rubia cordifolia: Structure-Radical Scavenging Activity Relationships, Journal of Agricultural and Food Chemistry. 2004, 52: 7884-7890. |
| Luo Q., Cai Y., Yan J., Sun M. and Corke H., Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum, Life Sciences. 2004, 76: 137-149. |
| Researcher : Chak KF |
| List of Research Outputs |
| Zhou Y., Chan J.H., Chan A.Y., Chak K.F., Wong Y.L., Chye M.L., Peiris J.S., Poon L.L. and Lam E., Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells, FEBS Letters. 2004, 577: 345-350. |
| Researcher : Chan SW |
| List of Research Outputs |
| Chan S.W., Identification of mutants in genes encoding Arabidopsis ACYL-Coenzyme a binding proteins ACBP3, ACBP4 and ADBP5, 2004. |
| Researcher : Chaturvedi G |
| List of Research Outputs |
| Chaturvedi G., Functional Characterization of OsELF3 as a flowering time regulator of rice, 2005. |
| Researcher : Chen SF |
| Project Title: | Transgenic chlamydomonas reinhardtii as an experimental system to study astaxanthin biosynthesis in green microalgae |
| Investigator(s): | Chen SF, Huang J, Sandmann G |
| Department: | Botany |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 09/2003 |
| Abstract: |
| To construct plasmids contianing single bkt ([beta]-carotene ketolase), and bhy ([beta]-carotene hydroxylase) under the control of a synthetic inducible promoter, and construct a bi-directional polar promoter for directing the expression of bkt and bhy; to introduce the gene bkt and/or bhy into C. reinhardtii; to investigate the expression of transgenes in C. reinhardtii; to study the molecular aspects of astaxanthin biosysthesis; and to investigate growth kinetics and productivity of astaxanthin of the Chlamydomonas mutants under photoautotrophic, mixotrophic and heterotrophic culture conditions. |
| Project Title: | Transgenic chlamydomonas reinhardtii as an experimental system to study astaxanthin biosynthesis in green microalgae |
| Investigator(s): | Chen SF, Huang J, Sandmann G |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2003 |
| Abstract: |
| To construct plasmids contianing single bkt ([beta]-carotene ketolase), and bhy ([beta]-carotene hydroxylase) under the control of a synthetic inducible promoter, and construct a bi-directional polar promoter for directing the expression of bkt and bhy; to introduce the gene bkt and/or bhy into C. reinhardtii; to investigate the expression of transgenes in C. reinhardtii; to study the molecular aspects of astaxanthin biosysthesis; and to investigate growth kinetics and productivity of astaxanthin of the Chlamydomonas mutants under photoautotrophic, mixotrophic and heterotrophic culture conditions. |
| Project Title: | Enhanced biosynthesis of astaxanthin in the green microalga Chlorella zofingiensis |
| Investigator(s): | Chen SF |
| Department: | Botany |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To investigate the biosynthesis of the ketocarotenoid astaxanthin in mixotrophic or heterotrophic culture of the microalga C. zofingiensis in which glucose and nitrate are manipulated to facilitate high-yield production of the pigment. |
| Project Title: | New antiparasitic molecules from microalgae |
| Investigator(s): | Chen SF, Vivares C. |
| Department: | Botany |
| Source(s) of Funding: | France/Hong Kong Joint Research Scheme - Travel Grants |
| Start Date: | 01/2004 |
| Abstract: |
| To obtain new antiparasitic molecules from microalgae from natural sources. |
| Project Title: | Molecular analyses of astaxanthin biosynthesis in the green microalgae Haematococcus pluvialis and Chlorella zofingiensis: a comparative study |
| Investigator(s): | Chen SF |
| Department: | Botany |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2004 |
| Abstract: |
| Refer to hard copy |
| List of Research Outputs |
| Chen S.F., Jiang Y. and Ouyang F., Development of bioprocess engineering in China, Biotechnology. 2005, 4: 1-6. |
| Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75. |
| Chen S.F., Invited Lecture, Invited lecture at University of Blaise Pascal, Clermont Ferrand, France . 2004. |
| Chen S.F., Invited Lecture, Invited lecture in Inje University, South Korea. 2004. |
| Chen S.F., Invited Speech, New Zealand Institute of Food Science and Technology Annual Conference 2005. 2005. |
| Chen S.F., Li H., Wong R.N.S., Ji B. and Jiang Y., Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography, Journal of Chromatography A. 2005, 1064: 183-186. |
| Chen S.F., Keynote/Plenary Lecture, The 4th International Chlorella Symposium, Functionality of Chlorella and Its Industrial Application. 2004. |
| Chen S.F., Liu X., Ma K.K. and Jiang Y., Nutrient profiles of an edible cyanobacterium Nostoc flagelliforme, Conference Handbook of the NZIFST Annual Conference 2005. 28-30 June. Christchurch, New Zealand, 2005, p. 40. |
| Chen S.F., Outstanding Researcher in Food Science and Technology Award , Chinese Institute of Food Science and Technology. 2004. |
| Chen S.F., The high-value green microscopic vegetable Chlorella and its biotechnological potential, Conference Handbook of the NZIFST Annual Conference 2005. 28-30 June. Christchurch, New Zealand, 2005, p. 21. |
| Chen S.F., The versatile microalgae, Chlorella and their biotechnological potential, Proceedings of the 4th International Chlorella Symposium, 10 November 2004. Seoul, South Korea, p. 1-24. |
| Huang Z., Guo B.J., Chen S.F., Wong R.N.S. and Jiang Y., Selenium-binding phycocyanin from Spirulina platensis: selenium distribution and scavenging activity, Conference Handbook of the NZIFST Annual Conference 2005. 28-30 June. Christchurch, New Zealand, 2005, p. 40. |
| Ip P.F., Wong K.H. and Chen S.F., Enhanced production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture, Process Biochemistry. 2004, 39(11): 1761-1766. |
| Ip P.F. and Chen S.F., Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark, Process Biochemistry. 2005, 40: 733-738. |
| Kumar S., Cheung H.Y., Lau W.C.S., Chen S.F. and Hyde K.D., In vitro studies of endophytic fungi from Tripterygium wilfordii with anti-proliferative activity on human peripheral blood mononuclear cells, Journal of Ethnopharmacology. 2004, 94: 295-300. |
| Li H. and Chen S.F., Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed-counter-current chromatography, Journal of Chromatography A. 2005, 1074: 107-110. |
| Li H. and Chen S.F., Preparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase, Journal of Chromatography A. 2004, 1061: 51-54. |
| Li H. and Chen S.F., Preparative isolation and purification of chuanxiongzine from medicinal plant Ligusticum chuanxiong by high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1047: 249-253. |
| Li H. and Chen S.F., Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1052(1-2): 229-232. |
| Li H., Jiang Y. and Chen S.F., Separation methods used for Scutellaria baicalensis active components, Journal of Chromatography B. 2004, 812: 277-290. |
| Li H. and Chen S.F., Simultaneous separation and purification of five coumarins from the Chinese medicinal herb Cnidium monnieri by high-speed counter-current chromatography, Journal of Separation Science. 2005, 28: 268-272. |
| Li X.Z., Zhang D.H., Chen S.F., Ma J., Dong Y.H. and Zhang L.H., Klebsiella singaporensis sp. nov., a novel isomaltulose-producing bacterium., International Journal of Systematic and Evolutionary Microbiology. 2004, 54: 2131-2136. |
| Liu X., Jiang Y. and Chen S.F., Fatty acid profile of the edible filamentous cyanobacterium Nostoc flagelliforme at different temperatures and developmental stages in liquid suspension culture, Process Biochemistry. 2005, 40: 371-377. |
| Lu H.T., Li H., Chen S.F., Wong M.H. and Jiang Y., Determination of triphenyltin and its metabolite diphenyltin by high-performance liquid chromatography with UV detection, Chromatographia. 2004, 60: 113-116. |
| Lu H.T., Jiang Y., Li H., Chen S.F. and Wong M.H., Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection, Chromatographia. 2004, 60: 259-264. |
| Wang S., Chen S.F., Sommerfeld M. and Hu Q., Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae), Planta. 2004, 220: 17-29. |
| Wen Z.Y. and Chen S.F., Prospects for eicosapentaenoic acid production using microorganisms, In: Cohen, Z. & Ratledge, C. (eds), Single Cell Oils. Champaign, AOCS Press, 2005, pp. 138-160 (invited paper). |
| Researcher : Cheung YW |
| List of Research Outputs |
| Cheung Y.W., Isolation and Identification of B-Cyanalanine Synthase From Etoilated Rice (Oryza sativa) Seedlings, 2004. |
| Researcher : Choi SM |
| List of Research Outputs |
| Choi S.M., Study of Structure-Function Relationships in Globulin from common buckwheat (Fagopyrum esculentum Monench) seeds, 2004. |
| Researcher : Chye ML |
| Project Title: | Production of fungus-resistant transgenic potato |
| Investigator(s): | Chye ML |
| Department: | Botany |
| Source(s) of Funding: | Areas of Excellence Scheme |
| Start Date: | 03/2000 |
| Abstract: |
| To produce fungus-resistant transgenic potato. |
| Project Title: | Functional analysis of Arabidopsis acyl-CoA binding proteins |
| Investigator(s): | Chye ML |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2000 |
| Abstract: |
| To elucidate the function of Arabidopsis ACBP1 and ACBP2. Findings from this study could potentially lead to the manipulation of plant lipid transport/storage in oilseed crops. |
| Project Title: | Regulation of cysteine proteinase expression in Solanum melongena |
| Investigator(s): | Chye ML, Yao KM |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2001 |
| Completion Date: | 08/2004 |
| Abstract: |
| To elucidate the molecular basis for ethylene- induction and circadian regulation of SmCP, a gene encoding cysteine proteinase that functions at senescence and developmental events associated with programmed cell death (PCD) in Solanum melongena (brinjal/ eggplant/ aubergine). |
| Project Title: | Subcellular localization and mutational analysis of Brassica juncea HMGS |
| Investigator(s): | Chye ML, Bach T.J. |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 12/2002 |
| Completion Date: | 05/2005 |
| Abstract: |
| The project attempts to localize BjHMGS by using: 1) anti-BjHMGS antibodies in western blot analysis and immunoelectron microscopy; and 2) BjHMGS-Green Fluorescent Protein fusions. The research team will also identify the amino acid residues in BhHMGS that are essential for catalytic activity by site-directed in vitro mutagenesis. This would be a prerequisite to the crystallization of BjHMGS and the elucidation of its 3-D structure and interaction with substrates, inhibitors and other factors affecting its biological activity. |
| Project Title: | Analysis of the catalytic domain in Brassica chitinase |
| Investigator(s): | Chye ML, Mowbray S. |
| Department: | Botany |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Completion Date: | 10/2004 |
| Abstract: |
| To elucidate the amino acids that are crucial for activity at the catalytic domain of BjCHI1. |
| Project Title: | Functional analysis of novel arabidopsis ACBPs containing kelch motifs |
| Investigator(s): | Chye ML |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2004 |
| Abstract: |
| To establish the function of these novel ACBPs by investigating: (1) the function of their acyl-CoA binding domains in preferentially binding oleoyl-CoA. (2) the significance of their kelch motifs in mediating protein-protein interactions, to identify their protein partners which will further verify their roles in plant lipid metabolism. |
| Project Title: | Identification of phytopathogens susceptible to agglutinating chitinase BjCHI1 |
| Investigator(s): | Chye ML |
| Department: | Botany |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2004 |
| Abstract: |
| To identify phytopathogens that are susceptible to BjCHI1 action. |
| Project Title: | Functional analysis of novel arabidopsis ACBPs containing kelch motifs |
| Investigator(s): | Chye ML |
| Department: | Botany |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Abstract: |
| Refer to hard copy |
| List of Research Outputs |
| Chye M.L., Edward Clarence Dyason Universitas 21 Fellowship (2004/2005), 2004. |
| Chye M.L., Zhao K.J., He Z...M..., Ramalingam S. and Fung K.L., Expression of an agglutinating chitinase BjCHI1 with two chitin-binding domains is R. solani-inducible and confers fungal protection in transgenic potato, Planta. 2005, 220: 717-730. |
| Chye M.L. and Li H., Genetically Modified Food, 基因改造食物寓吃喝於防疫, Ming Pao Daily - Dec. 9. 明報, 2004. |
| Chye M.L., Li H., Ramalingam S., Poon L.L.M. and Peiris J.S.M., Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS, US Patent Application No. 11/004,399 filed December 3, 2004 [Patent WO2005054473-A1 published June 16,2005] . United States Patent Office, 2005. |
| Chye M.L., Xu Z. and Sin S.F., Genetically modified plants expessing proteinase inhibitors SaPIN2a or SaPIN2b, and methods of use thereof for the inhibition of trypsin- and chymotrypsin-like activities, US Patent Application No. 10/725,829 filed 1st December, 2003 [Patent 20040205846-A1 published October 14, 2004]. United States Patent Office, 2004. |
| Chye M.L., Investigations on an agglutinating chitinase from Brassica juncea (Indian mustard), School of Agriculture & Food Systems, University of Melbourne, Australia, May 20, 2005 (Invited lecture). 2005. |
| Lam E., Poon L.L.M., Zhou Y., Chye M.L. and Peiris J.S.M., Transgenic plant-derived siRNAs: suppression of influenza virus propagation in mammalian cells, US Provisional Patent Application No. 60/673,100 filed April 20, 2005 . 2005. |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, In CD and e-book "Proceedings of the 16th International Symposium on Plant Lipids", Budapest, Hungary. www.mete.mtesz.hu/pls P3-4 p.1-3. 2004, On-line Publication. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, Proceedings of the 16th International Symposium on Plant Lipids, Budapest, Hungary. p. 94. 2004. |
| Nagegowda D.A., Bach T.J. and Chye M.L., Expression of recombinant Brassica juncea HMG-CoA synthase 1 and its mutants, TERPNET 2005. 7th International meeting of research on terpenes and isoprenoids. Wageningen, The Netherlands. April 20-23, 2005. Poster 46, p. 104. 2005. |
| Nagegowda D.A., Bach T.J. and Chye M.L., Brassica juncea HMG-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes, Biochemical Journal. 2004, 383: 517-527. |
| Nagegowda D.A., Ramalingam S., Hemmerlin A., Bach T.J. and Chye M.L., Brassica juncea HMG-CoA synthase 1: localization of mRNA and protein, TERPNET 2005. 7th International meeting of research on terpenes and isoprenoids. Wageningen, The Netherlands. April 20-23, 2005. Poster 16, p. 74. 2005. |
| Rawat R. and Chye M.L., Identification of ethylene-responsive elements in the promoter of SmCP, the gene encoding Solanum melongena cysteine proteinase, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 754. 2004. |
| Rawat R., Xu Z.F., Yao K.M. and Chye M.L., Identification of cis-elements in ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase, Plant Molecular Biology. Springer, 2005, 57: 629-643. |
| Sin S.F. and Chye M.L., Expression of proteinase inhibitor II proteins during floral development in Solanum americanum flowers, Planta. 2004, 219: 1010-1022. |
| Tang C.M., Chye M.L., Ramalingam S., Ouyang S., Zhao K.J., Ubhayasekera W. and Mowbray S., Functional analysis of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1, Plant Molecular Biology. 2004, 56: 285-298. |
| Ubhayasekera W., Tang C.M., Berglund G., Bergfors T. and Chye M.L., Crystal structures of a family 19 chitinase from Brassica juncea show flexibility of binding cleft loops, Ninth Annual Conference of the Swedish Structural Biology Network, Tallberg, Sweden, June 17-20, 2005. Poster 11. 2005. |
| Zhou Y., Chan J.H., Chan A.Y., Chak K.F., Wong Y.L., Chye M.L., Peiris J.S., Poon L.L. and Lam E., Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells, FEBS Letters. 2004, 577: 345-350. |
| Researcher : Corke H |
| Project Title: | The diversity of cereal grain products in Hong Kong and southern China |
| Investigator(s): | Corke H |
| Department: | Botany |
| Source(s) of Funding: | Woo Ting Sang Agricultural Development Research Fund |
| Start Date: | 01/1996 |
| Abstract: |
| The cereal industry in this region is incredibly diverse. There is a vast expansion of diversity fueled by increasing consumer affluence in China, and by the need for convenience products. What attributes of the cereal grain raw materials are most relaated to quality of these products? What additives and modified starches will be in most demand in future? Without basic scientific analysis of products on the market, these questions cannot be answered. |
| Project Title: | Behavior of human incisor teeth in relation to sensory appreciation of texture |
| Investigator(s): | Corke H, Lucas W, Agrawal KR |
| Department: | Botany |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 03/2005 |
| Abstract: |
| IntroductionPeople obtain great enjoyment from eating their food. The enjoyment of food is a sensory pleasure that is appreciated from the cradle to the grave. The first bite, which provides a chance for teeth to contact the food, plays a critical role for people to assess the class of food. The incisor teeth used to cut or incise foods are the main agent for biting off a piece of food or cutting into small pieces when necessary. The fundamental mechanical parameters include fracture stress and strain, yield stress, elastic modulus, Poisson’s ratio, coefficient of friction and fracture toughness, which associated with texture in food products, are used to describe the food material characterization. In contrast to mastication, the resistance of a food to an incisal bite will depend basically on the square root of two major food properties, Young’s modulus (elastic modulus) and fracture toughness. For many foods, the quantity should be approximately equal to the product of the stress cracking during a bite and the square root of the length of a notch or indentation from which this crack initiates (index). There are many different kinds of starch-based foods with special good taste and abundant nutrition in Asian. Millions of people in Asian prefer to choose starch-based foods for the major food of their meal as well. To develop an instrumental method predicting the sensory perception of the textural properties of Asian starch-based foods have important significant to meet the need of the consumer’s preference. The aims of this study are to establish the dental variables responsible for variation in the index, and apply the methodology to two food systems – a model system of starch gels with widely varying textural properties and an applied system using a range of noodle formulations. Via simple but fundamentally grounded experiments, applications to both dental and food industry practice can be established.Literature ReviewThe acceptability of many classes of food to consumers is strongly influenced by expectations about food texture. Food texture is defined as “all the rheological and structural (geometric and surface) attributes of product perceptible by means of mechanical, tactile, and, where appropriate, visual and auditory receptors” (Lawless & Heymann, 1998). By this definition, food texture can be evaluated manually. There have been a large number of studies of incision by food scientists in relation to consumer preferences. Perceptions of fracture should be evaluated using teeth and the first bite with the incisors seems particularly critical in formulating opinions (Bourne, 2002). Mechanical elements are often simultaneously investigated by various instruments and then correlations are established between sensory terms and mechanical properties. Most analyses (Meullenet, 2002; Hylander, 1975) have centered on the correlation of psychophysical responses with the mechanical characteristics of foods obtained from tests made with universal testing machines. Significant correlations between a perceived food quality and a mechanical property do not imply a functional relationship. Materials must be homogeneous and isotropic, and be in a geometrical shape such that stresses and strains can be precisely calculated. If only a limited range of foods is offered, their mechanical properties would likely be correlated with each other (Ashby 1998). Any number of nonsensical conclusions might follow, which will only become apparent when predictions subsequently break down on a broader range of foods.However, food scientists have largely ignored the theory of fracture in their mechanical characterizations of starch-based food. There have been many more such studies on other foods, such as cheese, nuts, etc. Applying fracture mechanics, Vincent et al. (2002) found that the critical stress intensity factor, KIC, of foods, a parameter related to crack initiation, was linearly related to perceived measures of ‘hardness’ and ‘crunchiness’ as evaluated by trained ‘taste’ panelists. Vincent et al. suggested that mechanical characterizations of foods founded on sound theoretical support could replace the need for panels of this type since these are simpler and cheaper to run than taste panels and are free from cultural and linguistic limitations. The first bite has been instrumented before (Kohyama & Nishi 1997; Kohyama et al. 2001), but the use of the fracture theory and its potential for characterizing bite efficiency appears firmly linked to J.F.V. Vincent’s pioneering work. So applying the theory of fracture to the mechanical characterization of starch-based foods has very important significance. |
| List of Research Outputs |
| Bao J., Cai Y., Sun M., Wang G.Y. and Corke H., Anthocyanins, Flavonols, and Free Radical Scavenging Activity of Chinese Bayberry (Myrica rubra) Extracts and Their Color Properties and Stability, Journal of Agricultural and Food Chemistry. 2005, 53: 2327-2332. |
| Cai Y., Sun M., Xing J. and Corke H., Antioxidant Phenolic Constituents in Roots of Rheum officinale and Rubia cordifolia: Structure-Radical Scavenging Activity Relationships, Journal of Agricultural and Food Chemistry. 2004, 52: 7884-7890. |
| Luo Q., Cai Y., Yan J., Sun M. and Corke H., Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum, Life Sciences. 2004, 76: 137-149. |
| Qin F., Li J., Li X. and Corke H., AFLP and RFLP linkage map in Coix, Genetic Resources and Crop Evolution. 2005, 52: 209-214. |
| Researcher : Fan KW |
| List of Research Outputs |
| Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75. |
| Researcher : Fang X |
| List of Research Outputs |
| Fang X., Characteristics of extracts from Prunella vulagaria on the immune response of Monocytes/Macrophages, 2004. |
| Fang X., Chang R.C.C., Yuen W.H. and Zee S.S.Y., Immune modulatory effects of Prunella vulgaris L., International Journal of Molecular Medicine. 2005, 15: 491-496. |
| Researcher : Fang X |
| List of Research Outputs |
| Fang X., Characteristics of extracts from Prunella vulagaria on the immune response of Monocytes/Macrophages, 2004. |
| Fang X., Chang R.C.C., Yuen W.H. and Zee S.S.Y., Immune modulatory effects of Prunella vulgaris L., International Journal of Molecular Medicine. 2005, 15: 491-496. |
| Researcher : Fung KL |
| List of Research Outputs |
| Chye M.L., Zhao K.J., He Z...M..., Ramalingam S. and Fung K.L., Expression of an agglutinating chitinase BjCHI1 with two chitin-binding domains is R. solani-inducible and confers fungal protection in transgenic potato, Planta. 2005, 220: 717-730. |
| Researcher : Ip PF |
| List of Research Outputs |
| Ip P.F., Wong K.H. and Chen S.F., Enhanced production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture, Process Biochemistry. 2004, 39(11): 1761-1766. |
| Ip P.F. and Chen S.F., Production of astaxanthin by the green microalga Chlorella zofingiensis in the dark, Process Biochemistry. 2005, 40: 733-738. |
| Researcher : Lam E |
| List of Research Outputs |
| Lam E., Poon L.L.M., Zhou Y., Chye M.L. and Peiris J.S.M., Transgenic plant-derived siRNAs: suppression of influenza virus propagation in mammalian cells, US Provisional Patent Application No. 60/673,100 filed April 20, 2005 . 2005. |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Lo C.S.C., Wang N. and Lam E., Inducible double-stranded RNA expression activates reversible transcript turnover and stable translational suppression of a target gene in transgenic tobacco, FEBS Letters. 2005, 579: 1498-1502. |
| Wang N., Lo C.S.C. and Lam E., Construction of a recombinase-based cloning vector for inducible gene slencing in plants and its application in control of flowering, Plant Biology 2004, Lake Buena Vista, FL, USA, July 24-28 2004. |
| Zhou Y., Chan J.H., Chan A.Y., Chak K.F., Wong Y.L., Chye M.L., Peiris J.S., Poon L.L. and Lam E., Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells, FEBS Letters. 2004, 577: 345-350. |
| Researcher : Lee YT |
| List of Research Outputs |
| Lee Y.T., Production of Transgene Plant-Derived Vaccines Via Plastid Transformation Technology, 2004. |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Researcher : Lee YT |
| List of Research Outputs |
| Lee Y.T., Production of Transgene Plant-Derived Vaccines Via Plastid Transformation Technology, 2004. |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Researcher : Leung KC |
| List of Research Outputs |
| Leung K.C., Binding studies on Arabidopsis ACYL-Coenzyme a binding proteins ACBP3, ACBP4 ad ACBP5, 2004. |
| Researcher : Li H |
| List of Research Outputs |
| Chen S.F., Li H., Wong R.N.S., Ji B. and Jiang Y., Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography, Journal of Chromatography A. 2005, 1064: 183-186. |
| Chye M.L. and Li H., Genetically Modified Food, 基因改造食物寓吃喝於防疫, Ming Pao Daily - Dec. 9. 明報, 2004. |
| Chye M.L., Li H., Ramalingam S., Poon L.L.M. and Peiris J.S.M., Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS, US Patent Application No. 11/004,399 filed December 3, 2004 [Patent WO2005054473-A1 published June 16,2005] . United States Patent Office, 2005. |
| Li H. and Chen S.F., Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed-counter-current chromatography, Journal of Chromatography A. 2005, 1074: 107-110. |
| Li H. and Chen S.F., Preparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase, Journal of Chromatography A. 2004, 1061: 51-54. |
| Li H. and Chen S.F., Preparative isolation and purification of chuanxiongzine from medicinal plant Ligusticum chuanxiong by high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1047: 249-253. |
| Li H. and Chen S.F., Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1052(1-2): 229-232. |
| Li H., Jiang Y. and Chen S.F., Separation methods used for Scutellaria baicalensis active components, Journal of Chromatography B. 2004, 812: 277-290. |
| Li H. and Chen S.F., Simultaneous separation and purification of five coumarins from the Chinese medicinal herb Cnidium monnieri by high-speed counter-current chromatography, Journal of Separation Science. 2005, 28: 268-272. |
| Lu H.T., Li H., Chen S.F., Wong M.H. and Jiang Y., Determination of triphenyltin and its metabolite diphenyltin by high-performance liquid chromatography with UV detection, Chromatographia. 2004, 60: 113-116. |
| Lu H.T., Jiang Y., Li H., Chen S.F. and Wong M.H., Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection, Chromatographia. 2004, 60: 259-264. |
| Researcher : Li H |
| List of Research Outputs |
| Chen S.F., Li H., Wong R.N.S., Ji B. and Jiang Y., Isolation and purification of the bioactive carotenoid zeaxanthin from the microalga Microcystis aeruginosa by high-speed counter-current chromatography, Journal of Chromatography A. 2005, 1064: 183-186. |
| Chye M.L. and Li H., Genetically Modified Food, 基因改造食物寓吃喝於防疫, Ming Pao Daily - Dec. 9. 明報, 2004. |
| Chye M.L., Li H., Ramalingam S., Poon L.L.M. and Peiris J.S.M., Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS, US Patent Application No. 11/004,399 filed December 3, 2004 [Patent WO2005054473-A1 published June 16,2005] . United States Patent Office, 2005. |
| Li H. and Chen S.F., Isolation and purification of baicalein, wogonin and oroxylin A from the medicinal plant Scutellaria baicalensis by high-speed-counter-current chromatography, Journal of Chromatography A. 2005, 1074: 107-110. |
| Li H. and Chen S.F., Preparative isolation and purification of bergapten and imperatorin from the medicinal plant Cnidium monnieri using high-speed counter-current chromatography by stepwise increasing the flow-rate of the mobile phase, Journal of Chromatography A. 2004, 1061: 51-54. |
| Li H. and Chen S.F., Preparative isolation and purification of chuanxiongzine from medicinal plant Ligusticum chuanxiong by high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1047: 249-253. |
| Li H. and Chen S.F., Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography, Journal of Chromatography A. 2004, 1052(1-2): 229-232. |
| Li H., Jiang Y. and Chen S.F., Separation methods used for Scutellaria baicalensis active components, Journal of Chromatography B. 2004, 812: 277-290. |
| Li H. and Chen S.F., Simultaneous separation and purification of five coumarins from the Chinese medicinal herb Cnidium monnieri by high-speed counter-current chromatography, Journal of Separation Science. 2005, 28: 268-272. |
| Lu H.T., Li H., Chen S.F., Wong M.H. and Jiang Y., Determination of triphenyltin and its metabolite diphenyltin by high-performance liquid chromatography with UV detection, Chromatographia. 2004, 60: 113-116. |
| Lu H.T., Jiang Y., Li H., Chen S.F. and Wong M.H., Simultaneous determination of oxytetracycline, doxycycline, tetracycline and chlortetracycline in tetracycline antibiotics by high-performance liquid chromatography with fluorescence detection, Chromatographia. 2004, 60: 259-264. |
| Researcher : Liu X |
| List of Research Outputs |
| Chen S.F., Liu X., Ma K.K. and Jiang Y., Nutrient profiles of an edible cyanobacterium Nostoc flagelliforme, Conference Handbook of the NZIFST Annual Conference 2005. 28-30 June. Christchurch, New Zealand, 2005, p. 40. |
| Liu X., Jiang Y. and Chen S.F., Fatty acid profile of the edible filamentous cyanobacterium Nostoc flagelliforme at different temperatures and developmental stages in liquid suspension culture, Process Biochemistry. 2005, 40: 371-377. |
| Researcher : Lo CSC |
| Project Title: | Identification of proteins targeted for degradation in Arabidopsis thaliana during disease resistance responses |
| Investigator(s): | Lo CSC, Lam E |
| Department: | Botany |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 10/2003 |
| Abstract: |
| To employ two complementary technologies, namely exon-trapping and proteomics, to identify potential proteins that are targeted for degradation during defense responses to P. parasitica in Arabidopsis; to identify a list of candidate proteins for future characterizations. |
| Project Title: | Identification of proteins targeted for degradation in Arabidopsis thaliana during disease resistance responses |
| Investigator(s): | Lo CSC, Lam E |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 10/2003 |
| Abstract: |
| To employ two complementary technologies, namely exon-trapping and proteomics, to identify potential proteins that are targeted for degradation during defense responses to P. parasitica in Arabidopsis; to identify a list of candidate proteins for future characterizations. |
| Project Title: | Functional characterization of the chalcone synthase gene family in Sorghum bicolor |
| Investigator(s): | Lo CSC |
| Department: | Botany |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 02/2004 |
| Completion Date: | 04/2005 |
| Abstract: |
| To develop research tools and generate resources that will be useful and critical for complete characterization of the biological and biochemical functions of the individual CHS genes in sorghum. |
| Project Title: | Functional characterization and metabolic engineering of flavonoid pathways in rice |
| Investigator(s): | Lo CSC, Lam E |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 10/2004 |
| Abstract: |
| To study: (1) functional characterization of 8 selected homologs of flavonoid structural genes in rice i) complementation of Arabidopsis tt mutants ii) generation and analysis of rice gene suppression mutants. (2) generation of transgenic rice with enhanced and/or novel flavonoid metabolites in endosperm. |
| Project Title: | Functional characterization and metabolic engineering of flavonoid pathways in rice |
| Investigator(s): | Lo CSC |
| Department: | Botany |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Abstract: |
| Refer to hard copy |
| Project Title: | Gene discovery for the phytoalexin biosynthesis pathway in Sorghum bicolor |
| Investigator(s): | Lo CSC |
| Department: | Botany |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2005 |
| Abstract: |
| refer hard copy proposal |
| List of Research Outputs |
| Adachi K., DeZwaan T., Lo C.S.C., Montenegro-Chamorro M.V., Frank S., Darveaux B., Mahanty S., Heiniger R., Skalchunes A., Pan H., Tarpey R., Shuster J., Tanzer T. and Hamer L., Methods for the identification of inhibitors of threonine synthase as antibiotics, US Patent No. 6806060 (October 19, 2004). 2004. |
| Lo C.S.C., Wang N. and Lam E., Inducible double-stranded RNA expression activates reversible transcript turnover and stable translational suppression of a target gene in transgenic tobacco, FEBS Letters. 2005, 579: 1498-1502. |
| Lo C.S.C., Montenegro-Chamorro M.V., Frank S., Darveaux B., Mahanty S., Heiniger R., Skalchunes A., Pan H., Tarper R., Shuster J., Tanzer M.M., Hamer L., Adachi K. and DeZwaan T.M., Methods for the identification of inhibitors of asparagine synthase as antibiotics, US Patent No. 6852484 (February 8, 2005). 2005. |
| Wang N., Lo C.S.C. and Lam E., Construction of a recombinase-based cloning vector for inducible gene slencing in plants and its application in control of flowering, Plant Biology 2004, Lake Buena Vista, FL, USA, July 24-28 2004. |
| Yu K.Y., Springob K., Schmidt J., Nicholson R.L., Chu I.K., Yip W.K. and Lo C.S.C., A stilbene synthase gene (SbSTS1) is involved in host and nonhost defense responses in sorghum, Plant Physiology. 2005, 138: 393-401. |
| Yu K.Y., Lam N.W., Lo C.S.C. and Chu I.K., Qualitative and quantitative analysis of flavonoid metabolites in sorghum by liquid chromatograph tandem mass spectrometry, Poster Presentation in 12th Symposium on Chemistry Postgraduate Research in Hong Kong, City University of Hong Kong, April 23, 2005. Hong Kong, Poster No: AE-21. |
| Researcher : Lu H |
| List of Research Outputs |
| Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75. |
| Researcher : Lung RWM |
| List of Research Outputs |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Researcher : Ma CY |
| Project Title: | Structure-function study of rice seed proteins |
| Investigator(s): | Ma CY |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 12/2001 |
| Abstract: |
| To isolate and purify the four major Osborne protein fractions from rice seeds, and to conduct a detailed structural analysis of the proteins and their major subfractions; to study the physicochemical and thermal denaturation, aggregation and gelation properties of the rice storage proteins. |
| Project Title: | Application of raman spectroscopy for analysis of chemically modified food proteins |
| Investigator(s): | Ma CY |
| Department: | Botany |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2002 |
| Completion Date: | 10/2004 |
| Abstract: |
| Food proteins have been chemically modified by various methods to improve their functional properties to enhance their utilization as food ingredients in formulated foods. |
| Project Title: | Analysis of chemically modified non-starch polysaccharides by Raman spectroscopy |
| Investigator(s): | Ma CY |
| Department: | Botany |
| Source(s) of Funding: | Small Project Funding |
| Start Date: | 11/2003 |
| Abstract: |
| To apply the technique of Raman spectroscopy for determining degrees of chemical modification in proteins, and extend the technique to study modified non-starch polysaccharides. |
| Project Title: | Study of chemically modified food proteins by vibrational spectroscopy |
| Investigator(s): | Ma CY |
| Department: | Botany |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2004 |
| Abstract: |
| Refer to hard copy |
| List of Research Outputs |
| Ma C.Y., Soybean: soy concentrates and isolates, In: Encyclopedia of Grain Science (C.W. Wrigley, H. Corke, C.E. Walker, eds.). Oxford, Elsevier, 2004, pp. 168-174. |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Raman spectroscopic determination of extent of O-esterification in acetylated soy protein isolates, Food Chemistry. 2004, 87: 477-481. |
| Researcher : Mishra G |
| List of Research Outputs |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, In CD and e-book "Proceedings of the 16th International Symposium on Plant Lipids", Budapest, Hungary. www.mete.mtesz.hu/pls P3-4 p.1-3. 2004, On-line Publication. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, Proceedings of the 16th International Symposium on Plant Lipids, Budapest, Hungary. p. 94. 2004. |
| Researcher : Mishra G |
| List of Research Outputs |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, In CD and e-book "Proceedings of the 16th International Symposium on Plant Lipids", Budapest, Hungary. www.mete.mtesz.hu/pls P3-4 p.1-3. 2004, On-line Publication. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, Proceedings of the 16th International Symposium on Plant Lipids, Budapest, Hungary. p. 94. 2004. |
| Researcher : Nagegowda DA |
| List of Research Outputs |
| Nagegowda D.A., Bach T.J. and Chye M.L., Expression of recombinant Brassica juncea HMG-CoA synthase 1 and its mutants, TERPNET 2005. 7th International meeting of research on terpenes and isoprenoids. Wageningen, The Netherlands. April 20-23, 2005. Poster 46, p. 104. 2005. |
| Nagegowda D.A., Bach T.J. and Chye M.L., Brassica juncea HMG-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes, Biochemical Journal. 2004, 383: 517-527. |
| Nagegowda D.A., Ramalingam S., Hemmerlin A., Bach T.J. and Chye M.L., Brassica juncea HMG-CoA synthase 1: localization of mRNA and protein, TERPNET 2005. 7th International meeting of research on terpenes and isoprenoids. Wageningen, The Netherlands. April 20-23, 2005. Poster 16, p. 74. 2005. |
| Researcher : Nagegowda DA |
| List of Research Outputs |
| Nagegowda D.A., Bach T.J. and Chye M.L., Expression of recombinant Brassica juncea HMG-CoA synthase 1 and its mutants, TERPNET 2005. 7th International meeting of research on terpenes and isoprenoids. Wageningen, The Netherlands. April 20-23, 2005. Poster 46, p. 104. 2005. |
| Nagegowda D.A., Bach T.J. and Chye M.L., Brassica juncea HMG-CoA synthase 1: expression and characterization of recombinant wild-type and mutant enzymes, Biochemical Journal. 2004, 383: 517-527. |
| Nagegowda D.A., Ramalingam S., Hemmerlin A., Bach T.J. and Chye M.L., Brassica juncea HMG-CoA synthase 1: localization of mRNA and protein, TERPNET 2005. 7th International meeting of research on terpenes and isoprenoids. Wageningen, The Netherlands. April 20-23, 2005. Poster 16, p. 74. 2005. |
| Researcher : Ouyang S |
| List of Research Outputs |
| Tang C.M., Chye M.L., Ramalingam S., Ouyang S., Zhao K.J., Ubhayasekera W. and Mowbray S., Functional analysis of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1, Plant Molecular Biology. 2004, 56: 285-298. |
| Researcher : Ramalingam S |
| List of Research Outputs |
| Chye M.L., Zhao K.J., He Z...M..., Ramalingam S. and Fung K.L., Expression of an agglutinating chitinase BjCHI1 with two chitin-binding domains is R. solani-inducible and confers fungal protection in transgenic potato, Planta. 2005, 220: 717-730. |
| Chye M.L., Li H., Ramalingam S., Poon L.L.M. and Peiris J.S.M., Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS, US Patent Application No. 11/004,399 filed December 3, 2004 [Patent WO2005054473-A1 published June 16,2005] . United States Patent Office, 2005. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, In CD and e-book "Proceedings of the 16th International Symposium on Plant Lipids", Budapest, Hungary. www.mete.mtesz.hu/pls P3-4 p.1-3. 2004, On-line Publication. |
| Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, Proceedings of the 16th International Symposium on Plant Lipids, Budapest, Hungary. p. 94. 2004. |
| Nagegowda D.A., Ramalingam S., Hemmerlin A., Bach T.J. and Chye M.L., Brassica juncea HMG-CoA synthase 1: localization of mRNA and protein, TERPNET 2005. 7th International meeting of research on terpenes and isoprenoids. Wageningen, The Netherlands. April 20-23, 2005. Poster 16, p. 74. 2005. |
| Tang C.M., Chye M.L., Ramalingam S., Ouyang S., Zhao K.J., Ubhayasekera W. and Mowbray S., Functional analysis of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1, Plant Molecular Biology. 2004, 56: 285-298. |
| Researcher : Rawat R |
| List of Research Outputs |
| Rawat R., Characterization of the promoter of SmCP, the gene encoding Solanum melongena cysteine protenase, 2004. |
| Rawat R. and Chye M.L., Identification of ethylene-responsive elements in the promoter of SmCP, the gene encoding Solanum melongena cysteine proteinase, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 754. 2004. |
| Rawat R., Xu Z.F., Yao K.M. and Chye M.L., Identification of cis-elements in ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase, Plant Molecular Biology. Springer, 2005, 57: 629-643. |
| Researcher : Rawat R |
| List of Research Outputs |
| Rawat R., Characterization of the promoter of SmCP, the gene encoding Solanum melongena cysteine protenase, 2004. |
| Rawat R. and Chye M.L., Identification of ethylene-responsive elements in the promoter of SmCP, the gene encoding Solanum melongena cysteine proteinase, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 754. 2004. |
| Rawat R., Xu Z.F., Yao K.M. and Chye M.L., Identification of cis-elements in ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase, Plant Molecular Biology. Springer, 2005, 57: 629-643. |
| Researcher : Sin SF |
| List of Research Outputs |
| Chye M.L., Xu Z. and Sin S.F., Genetically modified plants expessing proteinase inhibitors SaPIN2a or SaPIN2b, and methods of use thereof for the inhibition of trypsin- and chymotrypsin-like activities, US Patent Application No. 10/725,829 filed 1st December, 2003 [Patent 20040205846-A1 published October 14, 2004]. United States Patent Office, 2004. |
| Sin S.F., Characterization of proteinase inhibitor II from Solanum americanum, 2004. |
| Sin S.F. and Chye M.L., Expression of proteinase inhibitor II proteins during floral development in Solanum americanum flowers, Planta. 2004, 219: 1010-1022. |
| Researcher : Sin SF |
| List of Research Outputs |
| Chye M.L., Xu Z. and Sin S.F., Genetically modified plants expessing proteinase inhibitors SaPIN2a or SaPIN2b, and methods of use thereof for the inhibition of trypsin- and chymotrypsin-like activities, US Patent Application No. 10/725,829 filed 1st December, 2003 [Patent 20040205846-A1 published October 14, 2004]. United States Patent Office, 2004. |
| Sin S.F., Characterization of proteinase inhibitor II from Solanum americanum, 2004. |
| Sin S.F. and Chye M.L., Expression of proteinase inhibitor II proteins during floral development in Solanum americanum flowers, Planta. 2004, 219: 1010-1022. |
| Researcher : Tang CM |
| List of Research Outputs |
| Tang C.M., Chye M.L., Ramalingam S., Ouyang S., Zhao K.J., Ubhayasekera W. and Mowbray S., Functional analysis of the chitin-binding domains and the catalytic domain of Brassica juncea chitinase BjCHI1, Plant Molecular Biology. 2004, 56: 285-298. |
| Researcher : Tsang JSH |
| Project Title: | Global analysis of burkholderia cepacia MBA4 growing on haloacid and alkanoic acid media |
| Investigator(s): | Tsang JSH |
| Department: | Botany |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2003 |
| Abstract: |
| To investigate the global response of MBA4 to haloacid. |
| Project Title: | Genomic analysis of the growth of Burkholderia cepacia MBA4 in haloacid and non-haloacid conditions |
| Investigator(s): | Tsang JSH |
| Department: | Botany |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 02/2004 |
| Completion Date: | 01/2005 |
| Abstract: |
| To understand the adaptation of the bacterium in different environment. |
| Project Title: | Isolation and characterisation of the regulator of the dehalogenase IVa gene of Burkholderia cepacia MBA4 |
| Investigator(s): | Tsang JSH |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 01/2005 |
| Abstract: |
| To identifiy the regulatory element; to confirm the functional role of the regulatory element; to purificate the regulatory protein(s); to characterise the regulatory protein(s); to determinate the DNA-binding site. |
| Project Title: | Isolation and characterisation of the regulator of the dehalogenase IVa gene of Burkholderia cepacia MBA4 |
| Investigator(s): | Tsang JSH |
| Department: | Botany |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 01/2005 |
| Abstract: |
| Refer to hard copy |
| List of Research Outputs |
| Schmidberger J.W., Oakley A.J., Tsang J.S.H. and Wilce M.C.J., Purification, crystallization and preliminary crystallographic analysis of DehIVa, a dehalogenase from Burkholderia cepacia MBA4, Acta Crystal F. 2005, 61: 271-273. |
| Tsang J.S.H., Molecular biology of the Burkholderia cepacia complex, Advances in Applied Microbiology. 2004, 54: 71-91. |
| Wong P.S.H. and Tsang J.S.H., Proteomic analysis of the dehalogenase producing Burkholderia cepacia, In Programme and abstracts of the BioScience 2004 from molecules to organisms, p. 148a. Glasgow, UK. 2004. |
| Yuen H.F. and Tsang J.S.H., A putative amino acid transporter is involved in the catabolite repression of the dehalogenase of Burkholderia cepacia MBA4, In Programme and abstracts of the BioScience 2004 from molecules to organisms, p. 147a. Glasgow, UK. 2004. |
| Researcher : Wang L |
| List of Research Outputs |
| Lung S.C., Chan W.L., Yip W.K., Wang L., Yeung E.C. and Lim B.L., Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization, Plant Science. 2005, 169: 341-349. |
| Researcher : Wang N |
| List of Research Outputs |
| Lo C.S.C., Wang N. and Lam E., Inducible double-stranded RNA expression activates reversible transcript turnover and stable translational suppression of a target gene in transgenic tobacco, FEBS Letters. 2005, 579: 1498-1502. |
| Wang N., Lo C.S.C. and Lam E., Construction of a recombinase-based cloning vector for inducible gene slencing in plants and its application in control of flowering, Plant Biology 2004, Lake Buena Vista, FL, USA, July 24-28 2004. |
| Researcher : Wang S |
| List of Research Outputs |
| Wang S., Chen S.F., Sommerfeld M. and Hu Q., Proteomic analysis of molecular response to oxidative stress by the green alga Haematococcus pluvialis (Chlorophyceae), Planta. 2004, 220: 17-29. |
| Researcher : Wong KH |
| List of Research Outputs |
| Ip P.F., Wong K.H. and Chen S.F., Enhanced production of astaxanthin by the green microalga Chlorella zofingiensis in mixotrophic culture, Process Biochemistry. 2004, 39(11): 1761-1766. |
| Researcher : Wong PSH |
| List of Research Outputs |
| Wong P.S.H. and Tsang J.S.H., Proteomic analysis of the dehalogenase producing Burkholderia cepacia, In Programme and abstracts of the BioScience 2004 from molecules to organisms, p. 148a. Glasgow, UK. 2004. |
| Researcher : Wong YL |
| List of Research Outputs |
| Zhou Y., Chan J.H., Chan A.Y., Chak K.F., Wong Y.L., Chye M.L., Peiris J.S., Poon L.L. and Lam E., Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells, FEBS Letters. 2004, 577: 345-350. |
| Researcher : Xing J |
| List of Research Outputs |
| Cai Y., Sun M., Xing J. and Corke H., Antioxidant Phenolic Constituents in Roots of Rheum officinale and Rubia cordifolia: Structure-Radical Scavenging Activity Relationships, Journal of Agricultural and Food Chemistry. 2004, 52: 7884-7890. |
| Researcher : Xu Z |
| List of Research Outputs |
| Chye M.L., Xu Z. and Sin S.F., Genetically modified plants expessing proteinase inhibitors SaPIN2a or SaPIN2b, and methods of use thereof for the inhibition of trypsin- and chymotrypsin-like activities, US Patent Application No. 10/725,829 filed 1st December, 2003 [Patent 20040205846-A1 published October 14, 2004]. United States Patent Office, 2004. |
| Researcher : Yau CP |
| List of Research Outputs |
| Zeidler M., Zhou Q., Sarda X., Yau C.P. and Chua N., The nuclear localization signal and the C-terminal region of FHY1 are required for transmission of phytochrome A signals, The Plant Journal. 2004, 40: 355-365. |
| Researcher : Yip WK |
| Project Title: | Physiological studies of tomato plants transformed with various antisense ACC synthase genes |
| Investigator(s): | Yip WK |
| Department: | Botany |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 09/2003 |
| Abstract: |
| To examine the gene silencing effects of ACS2 and ACS4 on tomato fruit ripening and development; to examine the effects of ACS1a and ACS6 on System I ethylene biosynthesis during tomato fruit development and ripening; to investigate auxin-induced ethylene physiological impacts by gene silencing of ACS3 and ACS5 in tomatoes. |
| Project Title: | Physiological studies of tomato plants transformed with various antisense ACC synthase genes |
| Investigator(s): | Yip WK |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 09/2003 |
| Abstract: |
| To examine the gene silencing effects of ACS2 and ACS4 on tomato fruit ripening and development; to examine the effects of ACS1a and ACS6 on System I ethylene biosynthesis during tomato fruit development and ripening; to investigate auxin-induced ethylene physiological impacts by gene silencing of ACS3 and ACS5 in tomatoes. |
| Project Title: | Characterization of a cyanide detoxification gene encoding L-3-cyanoalanine synthase from rice |
| Investigator(s): | Yip WK |
| Department: | Botany |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2004 |
| Abstract: |
| Project Title: | Functional characterization and subcellular localization of three ethylene receptors in rice |
| Investigator(s): | Yip WK, Yau CP |
| Department: | Botany |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 01/2005 |
| Abstract: |
| Ethylene is an important plant hormone which regulates a range of developmental and physiological process in plants. In the past decades, exceptional progress has been made on understanding the molecular mechanism controlling the ethylene signalling pathway which was shown to require membrane-associated ethylene receptors to function. In Arabidopsis, there are altogether five ethylene receptors which negatively regulate ethylene responses. However, little is known about the involvement of receptor genes in the ethylene perception of monocotyledonous plants such as rice. Recently, we have successfully isolated five putative ethylene receptor genes from rice and showed that their expression levels were regulated developmentally, and by various external stimuli including ethylene. Here we propose to functionally characterize three rice ethylene receptor genes, OS-ERS1, OS-ERS2 and OS-ETR2 in planta. Moreover recent studies on the subcellular localization of two ethylene receptors, At-ETR1 and NtHK1 (an ethylene receptor ortholog in tobacco) revealed that they were localized to endoplasmic reticulum and plasma membrane respectively. To understand more in detail how these three rice ethylene receptors function in cellular level, we propose to investigate their corresponding subcellular localization by fusing with fluorescent protein. |
| List of Research Outputs |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Lung S.C., Chan W.L., Yip W.K., Wang L., Yeung E.C. and Lim B.L., Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization, Plant Science. 2005, 169: 341-349. |
| Yu K.Y., Springob K., Schmidt J., Nicholson R.L., Chu I.K., Yip W.K. and Lo C.S.C., A stilbene synthase gene (SbSTS1) is involved in host and nonhost defense responses in sorghum, Plant Physiology. 2005, 138: 393-401. |
| Researcher : Yu KY |
| List of Research Outputs |
| Yu K.Y., Springob K., Schmidt J., Nicholson R.L., Chu I.K., Yip W.K. and Lo C.S.C., A stilbene synthase gene (SbSTS1) is involved in host and nonhost defense responses in sorghum, Plant Physiology. 2005, 138: 393-401. |
| Yu K.Y., Lam N.W., Lo C.S.C. and Chu I.K., Qualitative and quantitative analysis of flavonoid metabolites in sorghum by liquid chromatograph tandem mass spectrometry, Poster Presentation in 12th Symposium on Chemistry Postgraduate Research in Hong Kong, City University of Hong Kong, April 23, 2005. Hong Kong, Poster No: AE-21. |
| Researcher : Yu M |
| List of Research Outputs |
| Yu M., Identification and characterization of Ethylene Receptor Genes in Rice, 2005. |
| Researcher : Yuen HF |
| List of Research Outputs |
| Yuen H.F. and Tsang J.S.H., A putative amino acid transporter is involved in the catabolite repression of the dehalogenase of Burkholderia cepacia MBA4, In Programme and abstracts of the BioScience 2004 from molecules to organisms, p. 147a. Glasgow, UK. 2004. |
| Yuen H.F., A study of the Dehalogenase Iva gene of Burkholderia cepacia MBA4, 2004. |
| Researcher : Yuen SN |
| List of Research Outputs |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Raman spectroscopic determination of extent of O-esterification in acetylated soy protein isolates, Food Chemistry. 2004, 87: 477-481. |
| Researcher : Zee SSY |
| List of Research Outputs |
| Chang R.C.C., Yu M.S., Leung S.K.Y., Lai S.W., Che C.M., Zee S.S.Y., So K.F. and Yuen W.H., Neuroprotective effects of anti-aging Chinese Medicine Lycium barbarum against b-amyloid peptide neurotoxicity. (Best Poster Prize in Poster Session 2), International Congress on Complementary and Alternative Medicines (ICCAM) 2005, 26-28 February 2005, Singapore. 2005, 145. |
| Fang X., Chang R.C.C., Yuen W.H. and Zee S.S.Y., Immune modulatory effects of Prunella vulgaris L., International Journal of Molecular Medicine. 2005, 15: 491-496. |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Liu X., Lu Y., Zhu H., Xu X., Feng J. and Zee S.S.Y., Abnormal behavior of nuclei and microtubule organizational changes during embryo sac development in the poly-egg mutant, APIV of rice, Acta Botanica Sinica. 2004, 55: 547-556. |
| So K.F., Chan H.C., Chang R.C.C., Chan Y.M.S., Yuen W.H. and Zee S.S.Y., Neuroprotection and Chinese Herbal Extracts, International Symposium on Chemicals and Pharmaceutical, Guangzhou, China. 2004, 15. |
| Wang L., Liu X., Lu Y., Feng J., Xu X. and Zee S.S.Y., Endosperm Development in Autotetraploid Rice: The Development of the Cellulose Wall of Aleuronic Layer Cell, Starch Accumulation of Endosperm and Formation of a Callose "Sheath-like" Structure, Chinese Journal of Rice Science. 2004, 18(6): 507-514. |
| Wang L., Liu X., Lu Y., Feng J., Xu X. and Zee S.S.Y., Endosperm Development in Autotetraploid Rice: The Fusion of Polar Nuclei and the Formation of Endosperm Cell Wall, Chinese Journal of Rice Science. 2004, 18(4): 281-289. |
| Yu M.S., Leung K.Y., Lai S.W., Che C.M., Zee S.S.Y., So K.F., Yuen W.H. and Chang R.C.C., Neuroprotective effects of anti-aging oriental medicine Lycium barbarum against bamyloid peptide neurotoxicity, Experimental Gerontology. 2005, 40: 716-727. |
| Researcher : Zhao Y |
| List of Research Outputs |
| Zhao Y., Ma C.Y., Yuen S.N. and Phillips D.L., Raman spectroscopic determination of extent of O-esterification in acetylated soy protein isolates, Food Chemistry. 2004, 87: 477-481. |
| Researcher : Zhou Y |
| Project Title: | Production of antiviral signals in transgenic plants via expression of double-stranded RNA: a feasibility study |
| Investigator(s): | Zhou Y |
| Department: | Botany |
| Source(s) of Funding: | Merit Award for RGC CERG Funded Projects |
| Start Date: | 10/2003 |
| Completion Date: | 07/2005 |
| Abstract: |
| To demonstrate that siRNAs targeting transcripts of an animal virus for cleavage can be produced in transgenic tobacco plants (we will omit tomato transformation sisnce this species requires substantially more tedious work in terms of tissue culture; to compare the efficacy of dsRNAs produced in the nucleus vs. plastids for transcript silencing in tobacco (again, we will omit tomato for our work because of the lack of sufficient support for manpower); to examine the feasibility of RNA and dsRNA uptake into the circulatory system of rabbits feeding on transgenic or wild-type tomatoes, as well as commercial animal feed with added synthetic siRNAs. |
| Project Title: | Production of antiviral signals in transgenic plants via expression of double-stranded RNA: a feasibility study |
| Investigator(s): | Zhou Y, Lam E |
| Department: | Botany |
| Source(s) of Funding: | Competitive Earmarked Research Grants (CERG) |
| Start Date: | 10/2003 |
| Completion Date: | 07/2005 |
| Abstract: |
| To demonstrate that siRNAs targeting transcripts of an animal virus for cleavage can be produced in transgenic tobacco plants; to explore if chloroplast genome can be engineered to produce siRNAs, and if yes, to compare the efficacy of dsRNAs produced in the nucleus vs. plastids for transcript silencing in tobacco; to examine the feasibility of RNA and dsRNA uptake into the circulatory system of rabbits feeding on transgenic or wild-type tomatoes, as well as commercial animal fed with added synthetic siRNAs. |
| Project Title: | Hunting for environment-friendly genes from a model plant arabidopsis |
| Investigator(s): | Zhou Y |
| Department: | Botany |
| Source(s) of Funding: | Seed Funding Programme for Basic Research |
| Start Date: | 02/2004 |
| Completion Date: | 07/2005 |
| Abstract: |
| To test the phytoremediation abilities of those genes in plants through genetic engineering, and put them into application in cleaning-up the contamined areas. |
| Project Title: | Chloroplast transformation for the production of siRNA and antigen against two kinds of hepatitis viruses |
| Investigator(s): | Zhou Y |
| Department: | Botany |
| Source(s) of Funding: | Incentive Award for RGC CERG Fundable But Not Funded Projects |
| Start Date: | 07/2004 |
| Completion Date: | 06/2005 |
| Abstract: |
| Refer to hard copy |
| List of Research Outputs |
| Lam E., Poon L.L.M., Zhou Y., Chye M.L. and Peiris J.S.M., Transgenic plant-derived siRNAs: suppression of influenza virus propagation in mammalian cells, US Provisional Patent Application No. 60/673,100 filed April 20, 2005 . 2005. |
| Lee Y.T., Lung R.W.M., Zhou Y., Chye M.L., Yip W.K., Zee S.S.Y. and Lam E., Regulated expression of the antigens against Hepatitis viruses and Epstein Barr Virus (EBV) in tobacco via stable plastid transformation, Plant Biology 2004 (American Society of Plant Biologists), Florida, USA, July 24-28, 2004. Abstract 731. 2004. |
| Zhou Y., Chan J.H., Chan A.Y., Chak K.F., Wong Y.L., Chye M.L., Peiris J.S., Poon L.L. and Lam E., Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells, FEBS Letters. 2004, 577: 345-350. |