Dept of Zoology

DEPARTMENT OF ZOOLOGY

Researcher : Au WL

List of Research Outputs

Ip C.Y., Au W.L. and Leung F.C.C., Characterization of the 5'-Flanking Transcriptional Regulatory Region of Chicken Growth Hormone Gene, Experimental Biology of Medicine. 2004, 229: 640-649.

Researcher : Bao J

List of Research Outputs

Bao J., Cai Y., Sun M., Wang G.Y. and Corke H., Anthocyanins, Flavonols, and Free Radical Scavenging Activity of Chinese Bayberry (Myrica rubra) Extracts and Their Color Properties and Stability, Journal of Agricultural and Food Chemistry. 2005, 53: 2327-2332.

Researcher : Cai Y

Project Title:	Investigating structure-activity relationships of natural phenolic antioxidants from traditional Chinese medicinal plants
Investigator(s):	Cai Y, Sun M, Corke H
Department:	Botany
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To investigate and elucidate the structure-antioxidant activity relationships of representative natural phenolic antioxidants from traditional Chinese medicinal plants associated with anticancer using the improved ABTS⋅+ method (TEAC assay) at the basis of chemical structural analysis.

Researcher : Chan DKO

List of Research Outputs

Yang X., Sit W.H., Chan D.K.O. and Wan J.M.F., The cell death process of the anticancer agent polysaccharide-peptide (PSP) in human promyelocytic leukemic HL-60 cells, Oncology Reports. 2005, 13: 1201-1210.

Researcher : Chan LY

List of Research Outputs

Chan L.Y. and Li E.T.S., Increased uncoupling protein 1 and 3 in diet-induced obese rats supplemented with bitter melon (Momordica charantia), Federation of American Societies for Experimental Biology (Abstract 287.2). 2005.

Researcher : Chan MCW

Project Title:	Establishment of primary culture of human airway epithelial cells, as an in-vitro model to study human infection of coronavirus/avian influenza virus
Investigator(s):	Chan MCW, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To establish a primary culture of human airway and alveolar epithelial cells, which can act as an in-vitro model to study human infection of coronavirus and avian influenza virus.

Researcher : Chan SK

List of Research Outputs

Tan-Un K.C., Chan S.K., Wong S.Y., Lee K.H., Kawasoe J., Zielenski J., Tsui L.C. and Tan W.C., Prevalence of CFTR Gene Mutations/Polymorphisms in East Asian Patients with Asthma, American Thoracic Society, ATS, San Diego May 2005 page A619. 2005.

Researcher : Chan SM

Project Title:	Vitellogenesis in the decapod crustacean: analysis of the shrimp vitellogenin gene and the hormonal control of shrimp vitellogenesis
Investigator(s):	Chan SM
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2002

Abstract:

The project attempts to: 1) characterize the shrimp vitelogenin; 2) clone the vitellogenin gene and cDNA; 3) study the expression of shrimp vitellogenin; and 4) study the hormonal effects of vitellogenesis.

Project Title:	The role of the GATA transcription factor in the regulation of the shrimp eyestalk crustacean hyperglycemic hormone A (CHH-A) and vitellogenin gene expression
Investigator(s):	Chan SM
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

To clone and characterize the GATA-TF from the shrimp; to analyze the expression of the GATA-TF in relation to the reproductive cycle; to investigate the role of GATA-TF in the regulation of CHH-A gene and vitellogenin gene.

Project Title:	The role of the GATA transcription factor in the regulation of the shrimp eyestalk crustacean hyperglycemic hormone A (CHH-A) and vitellogenin gene expression
Investigator(s):	Chan SM
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

Project Title:	The farneosic acid o-methyltransferase in the control of sex determination and reproduction: potential application in monosex culture and gonad maturation in shrimp aquaculture
Investigator(s):	Chan SM
Department:	Zoology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

List of Research Outputs

Chan S.M., Mak A.S.C., Choi C.L., Ma H.T., Hui J.H.L. and Tiu H.K., Vitellogenesis in the Red Crab, Charybdis feriatus: Contributions from Small Vitellogenin Transcripts (CfVg) and Farnesoic Acid Stimulation of CfVg Expression, New York Academy of Sciences. 2005, 1040: 74-79.

Chan S.M., Tiu H.K., Hui J.H.L., Kung S.Y., Tsang W.S. and Mak A.S.C., Vitellogenesis in the decapod crustaceans: implications from the characterization of shrimp vitellogenin, 15th ICCE, May 22-27, Boston Abstract P.58. 2005.

Chung P.K., Kung S.Y., Chu K.H. and Chan S.M., The heat shock cognate70 (hsc70) as a negative regulator of vitellogenin gene expression in the sand shrimp, Metapenaeus ensis 15th ICCE, May 22-27, Boston Abstract P.59. 2005.

Guan H., Mak A.S.C. and Chan S.M., Metapenaeus ensis Keystone Symposia, Colorado April 14-19, Double-stranded RNA induced gene silencing of the CHH/MIH/GIH neuropeptide gene family in sand shrimp. 2004, 108.

Kee F., Ng S.M., Vaudry H., Pang T.K.R., Lau H.Y., Chan S.M. and Chow B.K.C., Aspartic acid scanning mutation analysis of a goldfish growth hormone-releasing hormone (GHRH) receptor specific to the GHRHsalmon-like peptide, General and Comparative Endocrinology. 2005, 140: 41-51.

Lu L., Zhou S.Y., Chen C., Weng S.P., Chan S.M. and He J.G., Complete genome sequence analysis of an iridovirus isoloated from the orange-spotted grouper, Epinephelus coioides, Virology. 2005, 339: 81-100.

Mak A.S.C., Choi C.L., Tiu H.K., Hui J.H.L., He J.G., Tobe S.S. and Chan S.M., Vitellogenesis in the red crab Charybdis feriatus: Hepatopancreas-specific expression and farnesoic acid stimulation of vitellogenin gene expression, Molecular Reproduction and Development. 2005, 70: 288-300.

Tiu H.K. and Chan S.M., Effects of 20-hydroxyecdysone, farnesoic acid and other hormones, 15th ICCE, May 22-27, Boston Abstract P.126. 2005.

Tiu H.K., He J.G. and Chan S.M., Organization and expression study of the shrimp (Metapenacus ensis) putative 5-HT receptor: up-regulation in the brain by 5-HT, Gene. 2005, 353(1): 41-52.

Xie J., Lu L., Deng M., Weng S., Zhu J., Wu Y., Gan L., Chan S.M. and He J., Inhibition of reporter gene and Iridovirus-tiger frog virus in fish cell by RNA interference, Virology. 2005, 338(1): 43-52.

Researcher : Chan WL

List of Research Outputs

Chan W.L. and Lim B.L., Expression and characterization of Bacillus phytase in transgenic Arabidopsis, Acta Physiologiae Plantarum. 2004, 26 (3): 143-144.

Lung S.C., Chan W.L., Yip W.K., Wang L., Yeung E.C. and Lim B.L., Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization, Plant Science. 2005, 169: 341-349.

Researcher : Chan YW

List of Research Outputs

Wu H.Z., Chan Y.W., Wong K.M. and Leung F.C.C., Molecular Identification of Hog Cholera Viruses from Southern China, Journal of Animal and Veterinary Advances. 2005, 4: 497-501.

Researcher : Chan YY

List of Research Outputs

Chan Y.Y., Hoo L.C.R. and Chow B.K.C., Classification of functional motifs of the secretin receptor on signal transduction, Peptide & Protein Research. 2004, 6: 145-156.

Researcher : Chen Q

List of Research Outputs

Chen Q. and Li E.T.S., Reduced adiposity in bitter melon (Momordica charantia) fed rats is associated with lower tissue triglyceride and higher plasma catecholamines, British Journal of Nutrition. 2005, 93: 747-754.

Researcher : Cheng CY

List of Research Outputs

Lui W.Y., Mruk D.D. and Cheng C.Y., Interactions among IQGAP1, cdc42, and the cadherin/catenin protein complex regulate Sertoli-germ cell adherens junction dynamics in the testis, Journal of Cellular Physiology. 2005, 202: 49-66.

Researcher : Chow BKC

Project Title:	Secretin as a neuropeptide
Investigator(s):	Chow BKC, Yung WH
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2002

Abstract:

The project attempts to:- 1) study the distributions fo secretin and secretin receptor in the rat brain by in situ hybridization and immunocytochemical staining; 2) investigate the electrophysiological functions of secretin in regulating Purkinje cell functions, particularly on neuronal pathways and mechanisms; and 3) study the potential electrophysiological activities of secretin in discrete cells from other brain regions.

Project Title:	Regulation of the human secretin receptor gene
Investigator(s):	Chow BKC
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To investigate the interactions of GC motifs within the core promoter region of the hSR gene with Sp1 and Sp3. In addition, the connections between the Sp proteins, cytosine methylation and methylation imprinting will also be studied to elucidate the epigenetic regulation of expression in the hSR gene.

Project Title:	Mapping of human secretin and its receptor gene expression in human cerebellum
Investigator(s):	Chow BKC
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To demonstrate the presence of their transcripts in human cerebellum using real-time quantitative PCR and in situ hybridization, and the localization of secretin peptide by immunohistochemical staining and confocal microscopy.

Project Title:	A conditional knockout animal model for secretin
Investigator(s):	Chow BKC, Sham MH, Yung WH
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To develop an embryonic stem cell-line carrying the floxed secretin gene flanked by loxP sites; to generate secretin null mutant to investigate the physiology of a general secretin KO; to investigate the physiological effects of neuron-specific and Purkinje-specific secretin gene KO.

Project Title:	A conditional knockout animal model for secretin
Investigator(s):	Chow BKC
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Chan Y.Y., Hoo L.C.R. and Chow B.K.C., Classification of functional motifs of the secretin receptor on signal transduction, Peptide & Protein Research. 2004, 6: 145-156.

Chow B.K.C., Croucher Senior Research Fellow, Croucher Foundation. 2004.

Chow B.K.C., Grace Pickford Medal: , International Federation of Comparative Endocrine Societies (IFCES). 2005.

Chow B.K.C., Grace Pickford lecture, 15th International Congress of Comparative Endocrinology, Boston, U.S.A. 2005.

Ho S.N.S., Chu Y.S.J., Yung W.H. and Chow B.K.C., Expression and electrophysiological studies of secretin in the rat hypothalamic paraventricular nucleus, 34th Annual Meeting of the Society for Neuroscience, San Diego, U.S.A.. 2004.

Kee F., Ng S.M., Vaudry H., Pang T.K.R., Lau H.Y., Chan S.M. and Chow B.K.C., Aspartic acid scanning mutation analysis of a goldfish growth hormone-releasing hormone (GHRH) receptor specific to the GHRHsalmon-like peptide, General and Comparative Endocrinology. 2005, 140: 41-51.

Lee M.Y., Yung W.H., Chen L. and Chow B.K.C., Expression and spatial distribution of secretin and secretin receptor in human cerebellum, Neuroendocrinology. 2005, 16: 219-222.

Lee T.O., Tan-Un K.C., Lin M.C. and Chow B.K.C., Retinoic acid activates human secretin gene expression by Sp proteins and Nuclear Factor I in neuronal SH-SY5Y cells, Journal of Neurochemistry. 2005, 93: 339-350.

Siu K.Y., Sham M.H. and Chow B.K.C., Secretin, a known gastrointestinal peptide, is widely expressed during mouse embryonic development, Gene Expression Patterns . 2005, 5: 445-451.

Researcher : Chow YCK

List of Research Outputs

Yip C.W., Hon C.C., Zeng F., Chow Y.C.K. and Leung F.C.C., Prevalence of non-pneumonic infections with SARS-correlated virus, Lancet. 2004, 363: 1826-1827.

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Chu YSJ

List of Research Outputs

Ho S.N.S., Chu Y.S.J., Yung W.H. and Chow B.K.C., Expression and electrophysiological studies of secretin in the rat hypothalamic paraventricular nucleus, 34th Annual Meeting of the Society for Neuroscience, San Diego, U.S.A.. 2004.

Researcher : Chung PK

List of Research Outputs

Chung P.K., Kung S.Y., Chu K.H. and Chan S.M., The heat shock cognate70 (hsc70) as a negative regulator of vitellogenin gene expression in the sand shrimp, Metapenaeus ensis 15th ICCE, May 22-27, Boston Abstract P.59. 2005.

Researcher : Fu G

List of Research Outputs

Huo L., Fu G., Wang X., Ko K.W. and Wong A.O.L., Modulation of Calmodulin Gene Expression as a Novel Mechanism for Growth Hormone Feedback Control by Insulin-like Growth Factor in Grass Carp Pituitary Cells, Endocrinology. 2005, 146: 3821-3835.

Researcher : Fung SK

List of Research Outputs

Fung S.K. and Wong A.O.L., Grass carp activin: Molecular cloning and functional role in regulating growth hormone gene expression in grass carp pituitary cells, Program and Abstracts, 86th Annual Meeting of the Endocrine Society, Abstract P1-262, pp. 217. 2004.

Researcher : Guan H

List of Research Outputs

Guan H., Mak A.S.C. and Chan S.M., Metapenaeus ensis Keystone Symposia, Colorado April 14-19, Double-stranded RNA induced gene silencing of the CHH/MIH/GIH neuropeptide gene family in sand shrimp. 2004, 108.

Researcher : He M

List of Research Outputs

Li E.T.S., He M., Yau Y.C. and Anderson G.H., Ethnic specific body mass index cut-off points, Canadian Federation of Biological Societies 48th Annual meeting, Guelph, June 2005 (Abstract F63). 2005.

Researcher : Ho SNS

List of Research Outputs

Ho S.N.S., Chu Y.S.J., Yung W.H. and Chow B.K.C., Expression and electrophysiological studies of secretin in the rat hypothalamic paraventricular nucleus, 34th Annual Meeting of the Society for Neuroscience, San Diego, U.S.A.. 2004.

Researcher : Hon CC

List of Research Outputs

Yip C.W., Hon C.C., Zeng F., Chow Y.C.K. and Leung F.C.C., Prevalence of non-pneumonic infections with SARS-correlated virus, Lancet. 2004, 363: 1826-1827.

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Hoo LCR

List of Research Outputs

Chan Y.Y., Hoo L.C.R. and Chow B.K.C., Classification of functional motifs of the secretin receptor on signal transduction, Peptide & Protein Research. 2004, 6: 145-156.

Researcher : Hui JHL

List of Research Outputs

Chan S.M., Mak A.S.C., Choi C.L., Ma H.T., Hui J.H.L. and Tiu H.K., Vitellogenesis in the Red Crab, Charybdis feriatus: Contributions from Small Vitellogenin Transcripts (CfVg) and Farnesoic Acid Stimulation of CfVg Expression, New York Academy of Sciences. 2005, 1040: 74-79.

Chan S.M., Tiu H.K., Hui J.H.L., Kung S.Y., Tsang W.S. and Mak A.S.C., Vitellogenesis in the decapod crustaceans: implications from the characterization of shrimp vitellogenin, 15th ICCE, May 22-27, Boston Abstract P.58. 2005.

Mak A.S.C., Choi C.L., Tiu H.K., Hui J.H.L., He J.G., Tobe S.S. and Chan S.M., Vitellogenesis in the red crab Charybdis feriatus: Hepatopancreas-specific expression and farnesoic acid stimulation of vitellogenin gene expression, Molecular Reproduction and Development. 2005, 70: 288-300.

Researcher : Hui KH

List of Research Outputs

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Hui PY

List of Research Outputs

Hui P.Y., Sit W.H. and Wan J.M.F., Induction of S phase cell arrest and caspase activation by polysaccharide peptide isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cells, Oncology Reports. 2005, 14: 145-155.

Researcher : Huo L

List of Research Outputs

Huo L., Lee K.Y., Leung P.C. and Wong A.O.L., Goldfish Calmodulin: Molecular Cloning, Tissue Distribution, and Regulation of Transcript Expression in Goldfish Pituitary Cells, Endocrinology. 2004, 145: 5056-5067.

Huo L., Fu G., Wang X., Ko K.W. and Wong A.O.L., Modulation of Calmodulin Gene Expression as a Novel Mechanism for Growth Hormone Feedback Control by Insulin-like Growth Factor in Grass Carp Pituitary Cells, Endocrinology. 2005, 146: 3821-3835.

Researcher : Ip CY

List of Research Outputs

Ip C.Y., Au W.L. and Leung F.C.C., Characterization of the 5'-Flanking Transcriptional Regulatory Region of Chicken Growth Hormone Gene, Experimental Biology of Medicine. 2004, 229: 640-649.

Researcher : Jiang Y

List of Research Outputs

Zhou H., Jiang Y., Ko K.W., Li W. and Wong A.O.L., Paracrine regulation of growth hormone gene expression by gonadotrophin release in grass carp pituitary cells: functonal implications, molecular mechanisms and signal transduction, Journal of Molecular Endocrinology. 2005, 34: 415-432.

Researcher : Ko KW

List of Research Outputs

Huo L., Fu G., Wang X., Ko K.W. and Wong A.O.L., Modulation of Calmodulin Gene Expression as a Novel Mechanism for Growth Hormone Feedback Control by Insulin-like Growth Factor in Grass Carp Pituitary Cells, Endocrinology. 2005, 146: 3821-3835.

Zhou H., Jiang Y., Ko K.W., Li W. and Wong A.O.L., Paracrine regulation of growth hormone gene expression by gonadotrophin release in grass carp pituitary cells: functonal implications, molecular mechanisms and signal transduction, Journal of Molecular Endocrinology. 2005, 34: 415-432.

Researcher : Ko RCC

Project Title:	Prevalence of two protozoan pathogens, Girardia and Cryptosporidium, in the water supplies of Hong Kong
Investigator(s):	Ko RCC, Mak C, Wu R.S.S., Singh M.
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	10/2002

Abstract:

The study attempts to:- 1) determine the general prevalence of two organisms, Giardia intestinalis and Cryptosporidium parvum, in Hong Kong water supplies; 2) determine the major source of contamination; 3) determine, if any, the seasonal incidence of these two organisms in various water supplies.

Project Title:	Modulation of cell-mediated immune and inflammatory responses by Trichinella pseudospiralis during the acute muscle phase of infection- the roles of adhesion molecules and chemokines
Investigator(s):	Ko RCC, Mak C
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002

Abstract:

To further characterise the cell-mediated immune (systemic) response during the early phase of worm infection; to determine the roles of adhesion molecules and chemokines in suppression of inflammatory/immune response after the newborn larvae have invaded the striated muscles; to test the hypothesis that this species of nematode produce effector molecules to evade host's response.

Project Title:	Studies on some heat-induced genes of an intracellular parasitic nematode, Trichinella pseudospiralis II. Cloning and characterization of a homologue of translationally controlled tumour proteins (TCTP)
Investigator(s):	Ko RCC, Mak C
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	11/2004

Abstract:

To complete our preliminary experiments by cloning and sequencing the entire transcript of the translationally controlled tumour protein (TCTP) of Trichinella; to elucidate the gene structure; to express the recombinant protein; to assay the biological functions of the protein and to assess its role in the infectious process.

Researcher : Kung SY

List of Research Outputs

Chan S.M., Tiu H.K., Hui J.H.L., Kung S.Y., Tsang W.S. and Mak A.S.C., Vitellogenesis in the decapod crustaceans: implications from the characterization of shrimp vitellogenin, 15th ICCE, May 22-27, Boston Abstract P.58. 2005.

Chung P.K., Kung S.Y., Chu K.H. and Chan S.M., The heat shock cognate70 (hsc70) as a negative regulator of vitellogenin gene expression in the sand shrimp, Metapenaeus ensis 15th ICCE, May 22-27, Boston Abstract P.59. 2005.

Researcher : Lau HY

List of Research Outputs

Kee F., Ng S.M., Vaudry H., Pang T.K.R., Lau H.Y., Chan S.M. and Chow B.K.C., Aspartic acid scanning mutation analysis of a goldfish growth hormone-releasing hormone (GHRH) receptor specific to the GHRHsalmon-like peptide, General and Comparative Endocrinology. 2005, 140: 41-51.

Researcher : Law KM

List of Research Outputs

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Lee KY

List of Research Outputs

Huo L., Lee K.Y., Leung P.C. and Wong A.O.L., Goldfish Calmodulin: Molecular Cloning, Tissue Distribution, and Regulation of Transcript Expression in Goldfish Pituitary Cells, Endocrinology. 2004, 145: 5056-5067.

Researcher : Lee MY

List of Research Outputs

Lee M.Y., Yung W.H., Chen L. and Chow B.K.C., Expression and spatial distribution of secretin and secretin receptor in human cerebellum, Neuroendocrinology. 2005, 16: 219-222.

Researcher : Lee PY

List of Research Outputs

Lee P.Y., Ectoplasmic specialization, a testis-specific cell-cell actin-based adherens junction type: is this a potential target for male contraceptive development?, Human Reproduction Update. 2004.

Wong C.H., Xia W., Lee P.Y., Mruk D.D., Lee W.W.M. and Cheng C.Y., Regulation of Ectoplasmic Specialization Dynamics in the Seminiferous Epithelium by Focal Adhesion-Associated Proteins in Testosterone-Suppressed Rat Testes, Endocrinology. 2005, 146: 1192-1204.

Zhang J., Wong C.H., Xia W., Mruk D.D., Lee P.Y., Lee W.W.M. and Cheng C.Y., Regulation of Sertoli-Germ Cell Adherens Junction Dynamics via Changes in Protein-Protein Interactions of the N-Cadherin-b-Catenin Protein Complex which Are Possibly Mediated by c-Src and Myotubularin-Related Protein 2: An in Vivo Study Using an Androgen Suppression Model, Endocrinology. 2005, 146: 1268-1284.

Researcher : Lee TO

Project Title:	Transcriptional regulation of a nasopharyngeal carcinoma tumor suppressor: RASSF1A
Investigator(s):	Lee TO, Chow BKC
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To elucidate the basal transcription regulation mechanisms of RASSFIA in normal - RASSF1A-expressing and RASSF1A-non-expressing NPC cells; to fine map the core promoter region of RASSF1 gene; identify transcription factor(s) that regulates RASSF1A expression.

List of Research Outputs

Lee T.O., Tan-Un K.C., Lin M.C. and Chow B.K.C., Retinoic acid activates human secretin gene expression by Sp proteins and Nuclear Factor I in neuronal SH-SY5Y cells, Journal of Neurochemistry. 2005, 93: 339-350.

Researcher : Lee WWM

Project Title:	Analysis of the promoter sequence of rat and mouse testin
Investigator(s):	Lee WWM
Department:	Zoology
Source(s) of Funding:	The Population Council - General Award
Start Date:	07/1999

Abstract:

To analysis promoter sequence of rat and mouse testin.

Project Title:	Gas6 expression and tyrosine kinase phosphorlation in testicular cell lines
Investigator(s):	Lee WWM
Department:	Zoology
Source(s) of Funding:	The Population Council - General Award
Start Date:	08/1999

Abstract:

To study Gas6 expression and tyrosine kinase phosphorlation in testicular cell lines.

Project Title:	Disassembly of cell junctions in the testis
Investigator(s):	Lee WWM, Cheng C.Y.
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2001

Abstract:

To identify target genes that are involved in junction disassembly between testicular cells by an in vitro cadmium chloride disassembly model system. Peptides of 20-25 amino acids will be synthesized based on the extracellular domains of Cx-33 (a testis-specific GJ protein) and occludin (a TJ protein found in the testis). 2.a. These peptides and their corresponding antibodies will be used to perturb cell junction assembly in vitro. 2.b. The active peptide will be injested intratesticularly to assess its effects in vivo. To use antisense oligodeoxynucleotides (ODNs) specific to Cx33, a testi-specific GJ protein largely expressed by germ cells, to perturb the GJs between testicular cells. It is anticipated such a disruption will impair germ cell movement when communications between Sertoli and germ cells are disrupted. 3.a. Antisense ODNs will be screened in vitro by using Sertoli cell cultures and Sertoli-germ cell cocultures to select ODNs that can arrest the timely expression of Cx33. 3.b. Selected ODNs will be injected intratesticularly to assess their effects in the seminiferous epithelium in vivo.

Project Title:	Induction of germ cell loss by a synthetic occludin peptide and its mechanism of action: an in vivo model to study cell junction formation in the testis
Investigator(s):	Lee WWM, Lui WY, Cheng C.Y.
Department:	Zoology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To examine the mechanism by which occludin peptide induces germ cell loss in the seminiferous epithelium; to investigate the integrity of the BTB in the peptide-injected testis and correlate with the kinetics of testicular changes; to examine whether the recovery of the BTB following the occludin peptide-induced damage to the Sertoli cell-TJ barrier is regulated, at least in part, by TGF-[beta].

Project Title:	Differential expression and transcriptional regulation of the Tyro 3 receptor tyrosine kinases in the testis
Investigator(s):	Lee WWM
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2004

Abstract:

To characterize the suppressing region of -26/+126, and to compare the difference in the transcriptional mechanism of the other two members of Tyro 3 family.

Project Title:	Regulation of anchoring junction dynamics in the testis
Investigator(s):	Lee WWM, Lui WY, Siu KY, Cheng C.Y.
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2004

Abstract:

To identificate the signaling and scaffolding proteins at the apical ectoplasmic specilizations (ES) that associate with the integrin/laminin, nectin/afadin/ponsin and cadherin/catenin complexes; to characterize the singaling pathways utilized by the actin-based adherens junctions (integrin/lamini, nectin/afadin/ponsin and cadherin/catenin complexes) to regulate ES dynamics.

Project Title:	Regulation of anchoring junction dynamics in the testis
Investigator(s):	Lee WWM
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Lee C.Y.L., Xu J., Lee C.K.F., Luk J.M.C., Lee W.W.M. and Yeung W.S.B., Mechanism of actions of human oviduct derived embryotrophic factor-3 (ETF3) or iC3b on mouse preimplantation embryo development, The 37th Annual Meeting of The Society For The Study of Reproduction, University of British Columbia, Vancouver, Canada, August 1-4, 2004.

Lui W.Y., Sze K.L. and Lee W.W.M., Cell junction proteins in the testis: transcriptional regulation and targeted protein degradation, Plenary Lecture, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Lui W.Y., Cheng Y.H., Mruk D.D., Cheng C.H., Mo M.Y., Lee W.W.M. and Cheng C.Y., Inflammatory response in the liver is influenced by the testis -- a physiological link exists between the testis and the liver?, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Lui W.Y., Sze K.L. and Lee W.W.M., Sp1 and CREB promote nectin-2 transcription to regulate adherens junctions assembly in the testis, Annual Meeting of the Society for Endocrinology, UK, November 1-3 2004, London, UK. 2004.

Lui W.Y. and Lee W.W.M., cAMP Perturbs Inter-Sertoli Tight Junction Permeability Barrier In Vitro via its Effect on Proteasome-Sensitive Ubiquitination of Occludin, Journal of Cellular Physiology. 2005, 203: 564-572.

Lui W.Y. and Lee W.W.M., cAMP perturbs inter-Sertoli tight junction permeability barrier in vitro via its effect on proteasome-sensitive ubiquitination of occludin., The 44th American Society for Cell Biology Annual Meeting, Dec 4-8 2004, Washington, DC, USA. 2004.

Lui W.Y., Cheng Y.H., Mruk D.D., Cheng C.H., Mo M.Y., Lee W.W.M. and Cheng C.Y., a₂-Macroglobulin expression in the liver in response to inflammation is mediated by the testis, Journal of Endocrinology. 2005, 185: 497-505.

Ng K.M., So M.T. and Lee W.W.M., Expression of Rabbit Sex Hormone-Binding Globulin during Pregnancy and Prenatal Development and Identification of a Novel Isoform, Endocrinology. 2005, 146: 1965-1972.

Ng K.M., So M.T. and Lee W.W.M., Rabbit sex hormone-binding globulin: expression during embryonic development and identification of a noval exon-skipped isoform, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-Germ Cell Anchoring Junction Dynamics in the Testis Are Regulated by an Interplay of Lipid and Protein Kinases, The Journal of Biological Chemistry. 2005, 280: 25029-25047.

Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Wong C.H., Mruk D.D., Siu K.Y., Lee W.W.M. and Cheng C.Y., Cell-cell junction dynamics at the blood-testis barrier (BTB) are regulated by a2-macroglobulin via the c-Jun N-terminal kinase (JNK) signaling pathway, The 44th American Society for Cell Biology Annual Meeting, Dec 4-8 2004, Washington, DC, USA. 2004.

Wong C.H., Xia W., Lee P.Y., Mruk D.D., Lee W.W.M. and Cheng C.Y., Regulation of Ectoplasmic Specialization Dynamics in the Seminiferous Epithelium by Focal Adhesion-Associated Proteins in Testosterone-Suppressed Rat Testes, Endocrinology. 2005, 146: 1192-1204.

Xu J., Lee C.Y.L., Lee C.K.F., Kwok K.L., Lee W.W.M., Luk J.M.C. and Yeung W.S.B., Embryotrophic factor-3 from human oviductal cells enhances proliferation, suppresses apoptosis and stimulates the expression of the b1 submit of sodium-potassium ATPase in mouse embryos, Human Reproduction. 2004, 19(12): 2919-2926.

Zhang J., Wong C.H., Xia W., Mruk D.D., Lee P.Y., Lee W.W.M. and Cheng C.Y., Regulation of Sertoli-Germ Cell Adherens Junction Dynamics via Changes in Protein-Protein Interactions of the N-Cadherin-b-Catenin Protein Complex which Are Possibly Mediated by c-Src and Myotubularin-Related Protein 2: An in Vivo Study Using an Androgen Suppression Model, Endocrinology. 2005, 146: 1268-1284.

Researcher : Leong CWI

List of Research Outputs

Leong C.W.I., Tan-Un K.C. and Li E.T.S., Associations of adiponectin gene polymorphisms (T45G and G276T) with risk factors in healthy Chinese adults are gender-dependent, Federation of American Societies for Experimental Biology (Abstract LB245). 2005.

Researcher : Leung FCC

Project Title:	Development of a rapid high throughput RT-PCR assay to detect SARS-CoV
Investigator(s):	Leung FCC
Department:	Zoology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To develop a 96-wells RT-PCR platform assay for the detection of the coronavirus.

Project Title:	Cloning of the viral genes from the newly identified SARS coronavirus
Investigator(s):	Leung FCC
Department:	Zoology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To clone all the viral gene into vector and these cloned genes will be then be used as reagents by us and other as the first step for investigation.

Project Title:	Rapid accurate diagnostic assay for the SARS coronavirus
Investigator(s):	Leung FCC, Peiris JSM
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (Special Grants for Severe Acute Respiratory Syndrome Research)
Start Date:	09/2003
Completion Date:	08/2004

Abstract:

To develop an S gene based ELISA assay for serological assessment of the population after the initial outbreak of SARS and to further develop the current RT-PCR assay for diagnosis the SARS Coronavirus developed in our laboratory into a rapid accurate high through-put assay that can accommodate up to thousands of samples per day.

Project Title:	Molecular cloning of the chicken growth hormone receptor and the molecular characterization of the 5'-flanking region
Investigator(s):	Leung FCC
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	10/2003

Abstract:

To determine the molecular structure and nucleotide sequence of the chicken GHR gene and the multiple 5' UTR exon 1.

Project Title:	Molecular cloning of the chicken growth hormone receptor and the molecular characterization of the 5'-flanking region
Investigator(s):	Leung FCC
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	10/2003

Abstract:

To determine the molecular structure and nucleotide sequence of the chicken GHR gene and the multiple 5' UTR exon 1.

Project Title:	Isolation and characterization of a PCV2 virus causing postweaning multisystemic wasting syndrome in pigs
Investigator(s):	Leung FCC
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To isolate and characterize the PCV2 virus that causes the Postweaning multisystemic wasting syndrome (PMWS) in pigs.

Project Title:	Molecular characterization of the chicken infectious bursal disease virus (IBDV) induced apoptosis
Investigator(s):	Leung FCC
Department:	Zoology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

List of Research Outputs

Ip C.Y., Au W.L. and Leung F.C.C., Characterization of the 5'-Flanking Transcriptional Regulatory Region of Chicken Growth Hormone Gene, Experimental Biology of Medicine. 2004, 229: 640-649.

Leung F.C.C., Virus, influenza to SARS, Public Lecture for Hong Kong Museum of Science. 2004.

Li J., Wang Y. and Leung F.C.C., Cloning of chicken growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) and characterization of their spatial and temporal expression, Proceedings 15th International Congress Comparative Endocrinology, Boston, MA. p. 92. 2005.

Qin J.H., Leung F.C.C., Fung Y.S., Zhu D. and Lin B.C., Rapid authentication of ginseng species using microchip electrophoresis with laser-induced fluorescence detection, Analytical and Bioanalytical Chemistry . 2005, 381: 812-819.

Wang C.Y. and Leung F.C.C., Characterization of the chicken prolactin-releasing peptide receptor gene, Proceedings 15th International Congress Comparative Endocrinology, Boston, MA. p.133. 2005.

Wang Y., Li J. and Leung F.C.C., Chicken epidermal growth factor (EGF): cDNA cloning, genomic organization and characterization of its expression in the ovary, Proceedings 15th International Congress Comparative Endocrinology, Boston, MA. p.133. 2005.

Wang Y. and Leung F.C.C., DNA structure constraint is probably a fundamental factor inducing CpG deficiency in bacteria , Bioinformatic. 2004, 20: 3336-3345.

Wong Y.L., Wu H.Z. and Leung F.C.C., Molecular characterization of the Chinese isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the construction and characterization of the DNA vaccines, Journal of Animal and Veterinary Advances. 2005, 4: 63-70.

Wu H.Z., Chan Y.W., Wong K.M. and Leung F.C.C., Molecular Identification of Hog Cholera Viruses from Southern China, Journal of Animal and Veterinary Advances. 2005, 4: 497-501.

Yip C.W., Hon C.C., Zeng F., Chow Y.C.K. and Leung F.C.C., Prevalence of non-pneumonic infections with SARS-correlated virus, Lancet. 2004, 363: 1826-1827.

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Leung MF

List of Research Outputs

Leung M.F. and Lim B.L., Persistence of recombinant Bacillus beta-propeller phytaswe derived from transgenic tobacco in soils, Acta Physiologiae Plantarum. 2004, 26 (3): 119.

Researcher : Leung PC

List of Research Outputs

Gu J., Wang Y. and Leung P.C., A cryptic plasmid from Vibrio cholerae MP-1 isolated from Mai Po Nature Reserve and other plasmids from both pristine and polluted marine environments, Conference on the Microbiology of the Tropical Seas. December 13-15, 2004. Dona Paula, Goa, India. 2004, p. PMB(IT)-02.

Huo L., Lee K.Y., Leung P.C. and Wong A.O.L., Goldfish Calmodulin: Molecular Cloning, Tissue Distribution, and Regulation of Transcript Expression in Goldfish Pituitary Cells, Endocrinology. 2004, 145: 5056-5067.

Wang Y., Leung P.C. and Gu J., A cryptic plasmid from Vibrio cholerae mp-1 isolated from Mai Po Nature Reserve of Hong Kong, BioMicroWorld 2005 - International Conference on Environmental, Industrial and Applied Microbiology, March 14-18, 2005. Badajoz, Spain. 2005, p. 5.

Researcher : Li ETS

Project Title:	Nutritional benefits of dietary fiber supplementation in hospitalized geriatrics
Investigator(s):	Li ETS, Lui S.S.H., Dai D.L.K., Chiu S., Chan V., Dai D.L.K.
Department:	Zoology
Source(s) of Funding:	Other Funding Scheme
Start Date:	09/1997

Abstract:

To improve gentle bowel fitness, relieve constipation and improve serum lipid profile of institutionalized geriatric patients via dietary fiber supplementation.

Project Title:	An evaluation on the antioxidant effects of Lycium barbarum L. and its supplementation on cataract formation in rats
Investigator(s):	Li ETS
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To systematically examine the antioxidant properties of Lycium barbarum L. and evaluate the effect of its supplementation on cataract development in rats.

Project Title:	Dietary bitter melon reduces adiposity and enhances insulin sensitivity: a role for peroxisome proliferator-activated receptors?
Investigator(s):	Li ETS
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

Determine the effect of bitter melon supplementation on adiposity, glucose tolerance and endocrine profile of insulin resistant rats; determine the involvement of peroxisome proliferators-activated receptors and adipocyte-derived peptides; determine the involvement of sympathetic activity and uncoupling proteins from brown and white adipocytes, and skeletal muscle.

Project Title:	Dietary bitter melon reduces adiposity and enhances insulin sensitivity: a role for peroxisome proliferator-activated receptors?
Investigator(s):	Li ETS
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

Project Title:	Anti-cataract effects of Lycium barbarum L and Momordica charantia
Investigator(s):	Li ETS
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To systematically examine the antioxidant properties of Lycium barbarum L. and Momordica charantia and evaluate the effects of supplementation on cataract development in rats.

List of Research Outputs

Chan L.Y. and Li E.T.S., Increased uncoupling protein 1 and 3 in diet-induced obese rats supplemented with bitter melon (Momordica charantia), Federation of American Societies for Experimental Biology (Abstract 287.2). 2005.

Chen Q. and Li E.T.S., Reduced adiposity in bitter melon (Momordica charantia) fed rats is associated with lower tissue triglyceride and higher plasma catecholamines, British Journal of Nutrition. 2005, 93: 747-754.

Leong C.W.I., Tan-Un K.C. and Li E.T.S., Associations of adiponectin gene polymorphisms (T45G and G276T) with risk factors in healthy Chinese adults are gender-dependent, Federation of American Societies for Experimental Biology (Abstract LB245). 2005.

Li E.T.S., He M., Yau Y.C. and Anderson G.H., Ethnic specific body mass index cut-off points, Canadian Federation of Biological Societies 48th Annual meeting, Guelph, June 2005 (Abstract F63). 2005.

Po H.M. and Li E.T.S., Reduced adiposity in bitter melon fed rats is accompanied with changes in PPAR-Alpha expression, Canadian Federation of Biological Societies 48th Annual meeting, Guelph, June 2005 (Abstract W61). 2005.

Researcher : Li J

List of Research Outputs

Li J., Wang Y. and Leung F.C.C., Cloning of chicken growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) and characterization of their spatial and temporal expression, Proceedings 15th International Congress Comparative Endocrinology, Boston, MA. p. 92. 2005.

Wang Y., Li J. and Leung F.C.C., Chicken epidermal growth factor (EGF): cDNA cloning, genomic organization and characterization of its expression in the ovary, Proceedings 15th International Congress Comparative Endocrinology, Boston, MA. p.133. 2005.

Researcher : Li W

List of Research Outputs

Zhou H., Jiang Y., Ko K.W., Li W. and Wong A.O.L., Paracrine regulation of growth hormone gene expression by gonadotrophin release in grass carp pituitary cells: functonal implications, molecular mechanisms and signal transduction, Journal of Molecular Endocrinology. 2005, 34: 415-432.

Researcher : Lie Ken Jie MSF

List of Research Outputs

Lie Ken Jie M.S.F. and Lam N.W., Reaction of Mono-epoxidized Conjugated Linoleic Acid Ester with Boron Trifluoride Etherate Complex , Lipids. 2004, 39: 583-587.

Mishra G., Ramalingam S., Lie Ken Jie M.S.F. and Chye M.L., Characterization of a mutant in Arabidopsis ACBP2, Proceedings of the 16th International Symposium on Plant Lipids, Budapest, Hungary. p. 94. 2004.

Researcher : Lim BL

Project Title:	Acute infectious bursal disease in poultry: epidemiology of the disease, basis of virulence and improvement of vaccination
Investigator(s):	Lim BL, Muller H., Van den Berg T., Eterradossi N.
Department:	Zoology
Source(s) of Funding:	European Commission (EUROPA) - General Award
Start Date:	01/1999

Abstract:

To study: 1) development and harmonisation of the diagnostic tools; 2) molecular epidemiology of vvIBDV; 3) constitution of an IBDV genomic cDNA bank and generation of relevant full length cDNA's that could be used in the reverse genetics system to generate genetically engineered recombinant infectious viral clones; 4) harmonisation of nomenclature of IBDV strains based on molecular criteria; 5) pathogenesis of the disease: search for cell receptors and virulence markers - molecular basis of attenuation - cell-mediated immune response; 6) improvement of the vaccination protocols: reverse genetics system - DNA vaccination.

Project Title:	Studies on Bacillus phytases
Investigator(s):	Lim BL
Department:	Zoology
Source(s) of Funding:	Other Funding Scheme
Start Date:	09/2000

Abstract:

To close the genes of Bacillus phytases; to overexpress Bacillus phytases and to characterize their biochemical properties; to carry out agricultural applications of Bacillus phytases.

Project Title:	Identification and molecular cloning of infectious bursal disease virus (IBDV) receptors on chicken cells
Investigator(s):	Lim BL
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2000
Completion Date:	08/2004

Abstract:

To clone and identify the receptors of infectious bursal disease virus (IBDV) by both molecular and biochemical approaches.

Project Title:	Regulatory mechanism of the bacteriophage phi-105
Investigator(s):	Lim BL, Leung T.Y.C.
Department:	Zoology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To paint a clearer picture of the regulatory mechanism of this Bacillus bacteriophage.

Project Title:	Characterization of phytase-like proteins from Shewanella oneidensis and nostoc punctiforme
Investigator(s):	Lim BL
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2004

Abstract:

To study the biochemical propeties of two puttive proteins, they are Shewanella oneidensis and Nostoc punctiforme, including substrate specificity, pH / temperature profiles and thermostability and compare them with the Bacillus phytases; to explore their physiological functions beside phytate-hydrolyzing activity, since both putative proteins were substantially larger than Bacillus phytases.

Project Title:	Utilization of soil organic phosphorus by genetically modified plants
Investigator(s):	Lim BL, Koyama H.
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2004

Abstract:

To confirm whether the enhanced growth performance of the GM line is attributed to the utilization of soil IHIP by the secreted [beta]-propella phytase; to examine whether the GM plants have lower P requriement than the null lines and the wildtype; to examine whether the GM plants can utilized various salt forms of IHPs in soil. (Fe-phytate, Ca-phytate, Al-phytate); to examine the stability and the activity of the secreted phytase in soil ; to compare the abilities of 3 classes of phytases (BPP, HAP and PAP) in hydrolyzing IHP adsorbed to various soil components (A1, Fe and Ca precipitates, clays, etc); to examine the effects of organic acids (citrate, malate, oxalate) on the activities of the three classes of phytases (BPP, HAP and PAP); to create a phytase secreting Arabidopsis line that has elevated citrate acid secretion from root and compare its growth performance with the line that solely secretes phytase.

Project Title:	Utilization of soil organic phosphorus by genetically modified plants
Investigator(s):	Lim BL
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Berg T.P.V., Morales D., Eterradossi N., Rivallan G., Toquin D., Raue R., Zierenberg K., Zhang M.F., Zhu Y.P., Wang C.Q., Zheng H.J., Wang X., Chen G.C., Lim B.L. and Müller H., Assessment of genetic, antigenic and pathotypic criteria for the characterization of IBDV strains, Avian Pathology. 2004, 33: 470-476.

Chan W.L. and Lim B.L., Expression and characterization of Bacillus phytase in transgenic Arabidopsis, Acta Physiologiae Plantarum. 2004, 26 (3): 143-144.

Leung M.F. and Lim B.L., Persistence of recombinant Bacillus beta-propeller phytaswe derived from transgenic tobacco in soils, Acta Physiologiae Plantarum. 2004, 26 (3): 119.

Lim B.L., Mobilization of soil organic phosphorus by root excretion of phytases, Symposium lecture, The XVth International Symposium on Gnotobiology. Abstract pp.36, Tokyo, Japan, June 20-24, 2005.

Lim B.L., Mobilization of soil organic phosphorus by root excretion of phytases, Symposium lecture, The XVth International Symposium on Gnotobiology. Tokyo, Japan, June 20-24, 2005. . 2005.

Lung S.C., Chan W.L., Yip W.K., Wang L., Yeung E.C. and Lim B.L., Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization, Plant Science. 2005, 169: 341-349.

Researcher : Lui WY

Project Title:	Transcriptional regulation of mouse nectin-2 gene in Sertoli cells
Investigator(s):	Lui WY, Lee WWM
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To explore the regulatory mechanism of nectin-2 gene expression in the testis.

List of Research Outputs

Lui W.Y., Sze K.L. and Lee W.W.M., Cell junction proteins in the testis: transcriptional regulation and targeted protein degradation, Plenary Lecture, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Lui W.Y., Cheng Y.H., Mruk D.D., Cheng C.H., Mo M.Y., Lee W.W.M. and Cheng C.Y., Inflammatory response in the liver is influenced by the testis -- a physiological link exists between the testis and the liver?, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Lui W.Y., Mruk D.D. and Cheng C.Y., Interactions among IQGAP1, cdc42, and the cadherin/catenin protein complex regulate Sertoli-germ cell adherens junction dynamics in the testis, Journal of Cellular Physiology. 2005, 202: 49-66.

Lui W.Y., Sze K.L. and Lee W.W.M., Sp1 and CREB promote nectin-2 transcription to regulate adherens junctions assembly in the testis, Annual Meeting of the Society for Endocrinology, UK, November 1-3 2004, London, UK. 2004.

Lui W.Y. and Lee W.W.M., cAMP Perturbs Inter-Sertoli Tight Junction Permeability Barrier In Vitro via its Effect on Proteasome-Sensitive Ubiquitination of Occludin, Journal of Cellular Physiology. 2005, 203: 564-572.

Lui W.Y. and Lee W.W.M., cAMP perturbs inter-Sertoli tight junction permeability barrier in vitro via its effect on proteasome-sensitive ubiquitination of occludin., The 44th American Society for Cell Biology Annual Meeting, Dec 4-8 2004, Washington, DC, USA. 2004.

Lui W.Y., Cheng Y.H., Mruk D.D., Cheng C.H., Mo M.Y., Lee W.W.M. and Cheng C.Y., a₂-Macroglobulin expression in the liver in response to inflammation is mediated by the testis, Journal of Endocrinology. 2005, 185: 497-505.

Researcher : Lung SC

List of Research Outputs

Lung S.C., Chan W.L., Yip W.K., Wang L., Yeung E.C. and Lim B.L., Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization, Plant Science. 2005, 169: 341-349.

Researcher : Ma HT

List of Research Outputs

Chan S.M., Mak A.S.C., Choi C.L., Ma H.T., Hui J.H.L. and Tiu H.K., Vitellogenesis in the Red Crab, Charybdis feriatus: Contributions from Small Vitellogenin Transcripts (CfVg) and Farnesoic Acid Stimulation of CfVg Expression, New York Academy of Sciences. 2005, 1040: 74-79.

Researcher : Mak ASC

List of Research Outputs

Chan L.L., Hodgkiss I.J., Wan J.M.F., Lum J.H.K., Mak A.S.C., Sit W.H. and Lo S.C.L., Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: The use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction, Proteomics. 2004, 4: 3214-3226.

Chan L.L., Hodgkiss I.J., Lam P.K.S., Wan J.M.F., Chou H.N., Lum J.H.K., Lo G.Y., Mak A.S.C., Sit W.H. and Lo S.C.L., Use of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal blooms, Proteomics. 2005, 5: 1580-1593.

Chan S.M., Mak A.S.C., Choi C.L., Ma H.T., Hui J.H.L. and Tiu H.K., Vitellogenesis in the Red Crab, Charybdis feriatus: Contributions from Small Vitellogenin Transcripts (CfVg) and Farnesoic Acid Stimulation of CfVg Expression, New York Academy of Sciences. 2005, 1040: 74-79.

Chan S.M., Tiu H.K., Hui J.H.L., Kung S.Y., Tsang W.S. and Mak A.S.C., Vitellogenesis in the decapod crustaceans: implications from the characterization of shrimp vitellogenin, 15th ICCE, May 22-27, Boston Abstract P.58. 2005.

Guan H., Mak A.S.C. and Chan S.M., Metapenaeus ensis Keystone Symposia, Colorado April 14-19, Double-stranded RNA induced gene silencing of the CHH/MIH/GIH neuropeptide gene family in sand shrimp. 2004, 108.

Mak A.S.C., Choi C.L., Tiu H.K., Hui J.H.L., He J.G., Tobe S.S. and Chan S.M., Vitellogenesis in the red crab Charybdis feriatus: Hepatopancreas-specific expression and farnesoic acid stimulation of vitellogenin gene expression, Molecular Reproduction and Development. 2005, 70: 288-300.

Researcher : Mei J

List of Research Outputs

Mei J. and Xu R.J., Transient changes of transforming growth factor-b expression in the small intestine of the pig in association with weaning, British Journal of Nutrition. 2005, 93: 37-45.

Researcher : Mruk DD

List of Research Outputs

Lui W.Y., Cheng Y.H., Mruk D.D., Cheng C.H., Mo M.Y., Lee W.W.M. and Cheng C.Y., Inflammatory response in the liver is influenced by the testis -- a physiological link exists between the testis and the liver?, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Lui W.Y., Mruk D.D. and Cheng C.Y., Interactions among IQGAP1, cdc42, and the cadherin/catenin protein complex regulate Sertoli-germ cell adherens junction dynamics in the testis, Journal of Cellular Physiology. 2005, 202: 49-66.

Wong C.H., Mruk D.D., Siu K.Y., Lee W.W.M. and Cheng C.Y., Cell-cell junction dynamics at the blood-testis barrier (BTB) are regulated by a2-macroglobulin via the c-Jun N-terminal kinase (JNK) signaling pathway, The 44th American Society for Cell Biology Annual Meeting, Dec 4-8 2004, Washington, DC, USA. 2004.

Wong C.H., Xia W., Lee P.Y., Mruk D.D., Lee W.W.M. and Cheng C.Y., Regulation of Ectoplasmic Specialization Dynamics in the Seminiferous Epithelium by Focal Adhesion-Associated Proteins in Testosterone-Suppressed Rat Testes, Endocrinology. 2005, 146: 1192-1204.

Zhang J., Wong C.H., Xia W., Mruk D.D., Lee P.Y., Lee W.W.M. and Cheng C.Y., Regulation of Sertoli-Germ Cell Adherens Junction Dynamics via Changes in Protein-Protein Interactions of the N-Cadherin-b-Catenin Protein Complex which Are Possibly Mediated by c-Src and Myotubularin-Related Protein 2: An in Vivo Study Using an Androgen Suppression Model, Endocrinology. 2005, 146: 1268-1284.

Researcher : Ng KM

List of Research Outputs

Ng K.M., So M.T. and Lee W.W.M., Expression of Rabbit Sex Hormone-Binding Globulin during Pregnancy and Prenatal Development and Identification of a Novel Isoform, Endocrinology. 2005, 146: 1965-1972.

Ng K.M., So M.T. and Lee W.W.M., Rabbit sex hormone-binding globulin: expression during embryonic development and identification of a noval exon-skipped isoform, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Researcher : Ng KM

List of Research Outputs

Researcher : Ng SM

List of Research Outputs

Kee F., Ng S.M., Vaudry H., Pang T.K.R., Lau H.Y., Chan S.M. and Chow B.K.C., Aspartic acid scanning mutation analysis of a goldfish growth hormone-releasing hormone (GHRH) receptor specific to the GHRHsalmon-like peptide, General and Comparative Endocrinology. 2005, 140: 41-51.

Researcher : Ngai HHY

List of Research Outputs

Ngai H.H.Y., Sit W.H. and Wan J.M.F., The Nephroprotective Effects of the Herbal Medicine Preparation, WH30+, on the Chemical-Induced Acute and Chronic Renal Failure in Rats, The American Journal of Chinese Medicine. 2005, 33: 491-500.

Researcher : Ngan ESW

Project Title:	Sonic hedgehog (Shh) signaling in the enteric neural crest cells
Investigator(s):	Ngan ESW, Sham MH, Lui VCH, Tam PKH
Department:	Surgery
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005

Abstract:

refer hard copy proposal

Researcher : Pang TKR

List of Research Outputs

Kee F., Ng S.M., Vaudry H., Pang T.K.R., Lau H.Y., Chan S.M. and Chow B.K.C., Aspartic acid scanning mutation analysis of a goldfish growth hormone-releasing hormone (GHRH) receptor specific to the GHRHsalmon-like peptide, General and Comparative Endocrinology. 2005, 140: 41-51.

Researcher : Po HM

List of Research Outputs

Po H.M. and Li E.T.S., Reduced adiposity in bitter melon fed rats is accompanied with changes in PPAR-Alpha expression, Canadian Federation of Biological Societies 48th Annual meeting, Guelph, June 2005 (Abstract W61). 2005.

Researcher : Pon YL

List of Research Outputs

Pon Y.L., Auersperg N. and Wong A.S.T., Gonadotropins Regulate N-cadherin-mediated Human Ovarian Surface Epithelial Cell Survival at Both Post-translational and Transcriptional Levels through a Cyclic AMP/Protein Kinase A Pathway, The Journal of Biological Chemistry. 2005, 280: 15438-15448.

Pon Y.L., Auersperg N. and Wong A.S.T., Gonadotropins in the regulation of cadherin-mediated survival signals of ovarian surface epithelium, Proceedings Nature Biotechnology. 2005, 16: 134.

Pon Y.L. and Wong A.S.T., Gonadotropins: regulators of N-cadherin adhesion and inhibitors of cell survival in human ovarian surface epithelium, Proceedings Society of Endocrinology, Metabolism and Reproduction 16,S1. 2004.

Researcher : Sit WH

List of Research Outputs

Chan L.L., Hodgkiss I.J., Wan J.M.F., Lum J.H.K., Mak A.S.C., Sit W.H. and Lo S.C.L., Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: The use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction, Proteomics. 2004, 4: 3214-3226.

Chan L.L., Sit W.H., Hodgkiss I.J., Hsieh D.P. and Wan J.M.F., The use of species-specific and strain-specific peptides for the identification of PST-producing strains of Alexandrium minutum and A, tamarense, Congress Proteomics for Health, Switzerland. P.79. 2004.

Chan L.L., Hodgkiss I.J., Lam P.K.S., Wan J.M.F., Chou H.N., Lum J.H.K., Lo G.Y., Mak A.S.C., Sit W.H. and Lo S.C.L., Use of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal blooms, Proteomics. 2005, 5: 1580-1593.

Hui P.Y., Sit W.H. and Wan J.M.F., Induction of S phase cell arrest and caspase activation by polysaccharide peptide isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cells, Oncology Reports. 2005, 14: 145-155.

Ngai H.H.Y., Sit W.H. and Wan J.M.F., The Nephroprotective Effects of the Herbal Medicine Preparation, WH30+, on the Chemical-Induced Acute and Chronic Renal Failure in Rats, The American Journal of Chinese Medicine. 2005, 33: 491-500.

Wan J.M.F. and Sit W.H., Activation of caspase is independent to cyclin E expression in camptothecin-induced apoptosis in HL-60 cells, Cytometry 59A (1): 117 XXII ISAC congress, France. 2004.

Yang X., Sit W.H. and Wan J.M.F., Bivariate flow cytometric analysis of HL-60 cell cycle progression affected by polysaccharopeptide (PSP) from coriolus versicolor mycelia, Cytometry 59A (1): 118-119, XXII ISAC congress, France. 2004.

Yang X., Sit W.H., Chan D.K.O. and Wan J.M.F., The cell death process of the anticancer agent polysaccharide-peptide (PSP) in human promyelocytic leukemic HL-60 cells, Oncology Reports. 2005, 13: 1201-1210.

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Siu KY

Project Title:	Ectoplasmic specialization dynamics in the testis is regulated by focal adhesion complex-mediated signaling pathways
Investigator(s):	Siu KY, Lee WWM
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To investigate the downstream signaling pathway(s) underneath integrin and FAK that contribute to the regulation of ES dynamics.

List of Research Outputs

Siu K.Y., Sham M.H. and Chow B.K.C., Secretin, a known gastrointestinal peptide, is widely expressed during mouse embryonic development, Gene Expression Patterns . 2005, 5: 445-451.

Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-Germ Cell Anchoring Junction Dynamics in the Testis Are Regulated by an Interplay of Lipid and Protein Kinases, The Journal of Biological Chemistry. 2005, 280: 25029-25047.

Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Wong C.H., Mruk D.D., Siu K.Y., Lee W.W.M. and Cheng C.Y., Cell-cell junction dynamics at the blood-testis barrier (BTB) are regulated by a2-macroglobulin via the c-Jun N-terminal kinase (JNK) signaling pathway, The 44th American Society for Cell Biology Annual Meeting, Dec 4-8 2004, Washington, DC, USA. 2004.

Researcher : Siu KY

List of Research Outputs

Siu K.Y., Sham M.H. and Chow B.K.C., Secretin, a known gastrointestinal peptide, is widely expressed during mouse embryonic development, Gene Expression Patterns . 2005, 5: 445-451.

Researcher : Siu KY

List of Research Outputs

Siu K.Y., Sham M.H. and Chow B.K.C., Secretin, a known gastrointestinal peptide, is widely expressed during mouse embryonic development, Gene Expression Patterns . 2005, 5: 445-451.

Researcher : Siu KY

List of Research Outputs

Siu K.Y., Sham M.H. and Chow B.K.C., Secretin, a known gastrointestinal peptide, is widely expressed during mouse embryonic development, Gene Expression Patterns . 2005, 5: 445-451.

Researcher : So MT

List of Research Outputs

Ng K.M., So M.T. and Lee W.W.M., Expression of Rabbit Sex Hormone-Binding Globulin during Pregnancy and Prenatal Development and Identification of a Novel Isoform, Endocrinology. 2005, 146: 1965-1972.

Ng K.M., So M.T. and Lee W.W.M., Rabbit sex hormone-binding globulin: expression during embryonic development and identification of a noval exon-skipped isoform, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Researcher : Sun M

Project Title:	Comparative studies of selected medicinal plants from China and India: phylogenetic relationships, population genetics, and conservation
Investigator(s):	Sun M, Corke H, Cai Y
Department:	Zoology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005

Abstract:

Folk medicinal herbs are commonly used for treatment of many diseases. Both China and India are among the countries rich in medicinal plant resources. During long-term folk practices, the two different cultures have each identified a range of medicinal plants. For example, of the recorded 27,100/17,000 native species in the flora of China/India, 11,146/7500 species are reported to have some medicinal values, respectively (Hamilton 2002). Still many locally available species are neglected or under-utilized because of the limitations of traditional approaches, such as random selection or the "trial and error" method, in the discoveries of medicinal plants. Cross-cultural investigations will promote further exploration of local flora for identification of new species of medicinal properties to facilitate the discovery of new drugs. For example, on the lists of important or national priority medicinal plants in India, many of the species have numerous congeners native to China. Although in some genera the same species are utilized as medicinal herbs in both cultures, more frequently this is not the case. Therefore, new medicinal species in the flora of China/India could be easily identified simply by reference to the recognized medicinal species in the other culture, although whether the plants with identical Latin names in different flora are actually the same species need to be confirmed. Furthermore, phylogenetic analysis can be performed for the genera/families containing well-known medicinal species both within and between cultures, in order to identify their closest wild relatives which are more likely possess the same or similar bioactive compounds. The traditional approaches to medicinal herb identification and classification are largely dependent on morphological and anatomical characteristics, but many of such characteristics are affected by environmental and developmental factors. Modern chemical analytical techniques and tools have been extensively used for characterization and quantification of bioactive components in medicinal plants. Plant species or genera with close relationships usually contain similar chemical compounds. Recent advances in molecular techniques render DNA-based assays more advantageous than both morphological and chemical approaches in resolving phylogenetic relationships at various taxonomic levels. The molecular genetic methods are based on the genotype rather than the phenotype, and therefore the phylogenetically informative characters are not affected by environmental and physiological factors. Also, DNA samples can be prepared non-destructively from small pieces of plant tissues and subsequently PCR-amplified to yield sufficient amounts of template DNA for molecular genetic analysis. Thus DNA-based molecular markers have been extensively used for the study of genetic diversity and evolutionary relationships in plants, including medicinal herbs. However, an integrative approach, using both molecular marker techniques and modern chemical analytical tools, to the identification/discovery of medicinal plants is still rare. Through DNA polymorphism assay of selected genera/families that contain important medicinal plants, we may better understand their evolutionary relationships and gain insight into the distribution of certain medicinal active components in the genera or families and identify new sources of active components-containing medicinal plants through chemical assays.The main objectives of the research: o Comparative analysis of bioactive compounds in selected medicinal plants and their closest wild relatives.o Phylogenetic analysis of species relationships using molecular marker systems, including ISSR, AFLP and ITS at both population and species levels and more conservative DNA sequences at the genus/family levels;o Investigation of population structure, reproductive ecology and genetic diversity in the endangered medicinal species for developing conservation and management strategies for sustainable utilization of natural resources.

List of Research Outputs

Bao J., Cai Y., Sun M., Wang G.Y. and Corke H., Anthocyanins, Flavonols, and Free Radical Scavenging Activity of Chinese Bayberry (Myrica rubra) Extracts and Their Color Properties and Stability, Journal of Agricultural and Food Chemistry. 2005, 53: 2327-2332.

Cai Y., Sun M., Xing J. and Corke H., Antioxidant Phenolic Constituents in Roots of Rheum officinale and Rubia cordifolia: Structure-Radical Scavenging Activity Relationships, Journal of Agricultural and Food Chemistry. 2004, 52: 7884-7890.

Cribb P., Sun M. and Barretto G., Phaius tankervilleae and P. wallichii (Orchidaceae), a pair of confused species, KEW Bulletin. 2004, 59: 547-554.

Luo Q., Cai Y., Yan J., Sun M. and Corke H., Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum, Life Sciences. 2004, 76: 137-149.

Researcher : Sze KL

List of Research Outputs

Lui W.Y., Sze K.L. and Lee W.W.M., Cell junction proteins in the testis: transcriptional regulation and targeted protein degradation, Plenary Lecture, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Lui W.Y., Sze K.L. and Lee W.W.M., Sp1 and CREB promote nectin-2 transcription to regulate adherens junctions assembly in the testis, Annual Meeting of the Society for Endocrinology, UK, November 1-3 2004, London, UK. 2004.

Researcher : Tan-Un KC

Project Title:	Characterization of cytoglobin, a potential biomarker for the diagnosis of human liver fibrosis
Investigator(s):	Tan-Un KC, Ng IOL
Department:	Zoology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	05/2005

Abstract:

refer hard copy proposal

List of Research Outputs

Chan M.M.W., Tan-Un K.C., Ip M.S.M., Tsang K.W.T., Ho S.P., Ho J.C.M., Chan H. and Lam W.K., Lung Cancer Susceptibility and Polymorphism of Glutathione-S-transferase Genes in Hong Kong, Lung Cancer. 2004, 45(2): 155-160.

Lee T.O., Tan-Un K.C., Lin M.C. and Chow B.K.C., Retinoic acid activates human secretin gene expression by Sp proteins and Nuclear Factor I in neuronal SH-SY5Y cells, Journal of Neurochemistry. 2005, 93: 339-350.

Leong C.W.I., Tan-Un K.C. and Li E.T.S., Associations of adiponectin gene polymorphisms (T45G and G276T) with risk factors in healthy Chinese adults are gender-dependent, Federation of American Societies for Experimental Biology (Abstract LB245). 2005.

Tan-Un K.C., Chan S.K., Wong S.Y., Lee K.H., Kawasoe J., Zielenski J., Tsui L.C. and Tan W.C., Prevalence of CFTR Gene Mutations/Polymorphisms in East Asian Patients with Asthma, American Thoracic Society, ATS, San Diego May 2005 page A619. 2005.

Researcher : Tiu HK

List of Research Outputs

Chan S.M., Mak A.S.C., Choi C.L., Ma H.T., Hui J.H.L. and Tiu H.K., Vitellogenesis in the Red Crab, Charybdis feriatus: Contributions from Small Vitellogenin Transcripts (CfVg) and Farnesoic Acid Stimulation of CfVg Expression, New York Academy of Sciences. 2005, 1040: 74-79.

Chan S.M., Tiu H.K., Hui J.H.L., Kung S.Y., Tsang W.S. and Mak A.S.C., Vitellogenesis in the decapod crustaceans: implications from the characterization of shrimp vitellogenin, 15th ICCE, May 22-27, Boston Abstract P.58. 2005.

Mak A.S.C., Choi C.L., Tiu H.K., Hui J.H.L., He J.G., Tobe S.S. and Chan S.M., Vitellogenesis in the red crab Charybdis feriatus: Hepatopancreas-specific expression and farnesoic acid stimulation of vitellogenin gene expression, Molecular Reproduction and Development. 2005, 70: 288-300.

Tiu H.K. and Chan S.M., Effects of 20-hydroxyecdysone, farnesoic acid and other hormones, 15th ICCE, May 22-27, Boston Abstract P.126. 2005.

Tiu H.K., He J.G. and Chan S.M., Organization and expression study of the shrimp (Metapenacus ensis) putative 5-HT receptor: up-regulation in the brain by 5-HT, Gene. 2005, 353(1): 41-52.

Researcher : Tsang WS

List of Research Outputs

Chan S.M., Tiu H.K., Hui J.H.L., Kung S.Y., Tsang W.S. and Mak A.S.C., Vitellogenesis in the decapod crustaceans: implications from the characterization of shrimp vitellogenin, 15th ICCE, May 22-27, Boston Abstract P.58. 2005.

Researcher : Wan JMF

Project Title:	The role of free radicals and antioxidants in motor neuron degenerative disease
Investigator(s):	Wan JMF, Vacca-Galloway LL
Department:	Zoology
Source(s) of Funding:	Other Funding Scheme
Start Date:	07/1995

Abstract:

There are increasing evidence indicating the involvement of free radicals damage in many chronic diseases such as Parkinson's disease, Alzheimer's disease and neurondegenerative disease. By using a motor neuron degenerative disease mouse model, to investigate the roles of free radicals in the disease process and investigating whether antioxidants such as vitamins E and C can be any therapeutic use by naturalizing the free radicals.

Project Title:	Effects of tumor necrosis factor-[alpha]TNF-[alpha] on cyclins and related cell cycle proteins expressions in human tumor cell lines as determined
Investigator(s):	Wan JMF
Department:	Zoology
Source(s) of Funding:	Other Funding Scheme
Start Date:	07/1995

Abstract:

To investigate: a) the effect of TNF on cyclins: D, E, A, B expression by flow cytometry; b) the effect of TNF on P21, PCNA expression by cytometric studies; c) the effect of TNF on tumor cell lines proliferation and apoptosis studied by flow cytometry.

Project Title:	The effects of antioxidants on small cell lung cancer cell line, NCI-H446
Investigator(s):	Wan JMF
Department:	Zoology
Source(s) of Funding:	Other Funding Scheme
Start Date:	09/1995

Abstract:

Lung cancer is almost certainly the most common cancer in the world today. Over the past several years, work has focused on characterizing the prevention, inhibition and regression of lung cancer by [beta]-carotene, vitamin C and vitamin C which act as antioxidants. This study aims to investigate the antiproliferative potential of [beta]-carotene and retinoids by flow cytometry technology. The data will help us to understand how antioxidants prevent lung cancer formation and progression.

Project Title:	The effects of different dietary fatty acids on the development of mammary tumors in female fischer 344 rats
Investigator(s):	Wan JMF
Department:	Zoology
Source(s) of Funding:	Other Funding Scheme
Start Date:	09/1995

Abstract:

Exciting evidences demonstrated that the quality of dietary fatty acids, especially W-6 and W-3 polyunsaturated fatty acids affect the development of cancers such as the colon, breast, and prostate. This project aims to investigate the effect of saturated, monosaturated W-3, and W-6 polyunsaturated fatty acids on breast cancer cells proliferation by using flow cytometry technology. The data in this study will help us understand the mechanisms involved in more depth.

Project Title:	To determine the mechanisms of apoptosis of polysaccharide peptide (PSP) in human leukemic cell lines and human normal lymphocytes
Investigator(s):	Wan JMF
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

To determine the apoptotic pathways of PSP-induced apoptosis in various human leukemic cell eines; to identify the survival pathways in the human normal lymphocytes in response to PSP-induced apoptosis.

Project Title:	To determine the mechanisms of apoptosis of polysaccharide peptide (PSP) in human leukemic cell lines and human normal lymphocytes
Investigator(s):	Wan JMF
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

List of Research Outputs

Chan L.L., Hodgkiss I.J., Wan J.M.F., Lum J.H.K., Mak A.S.C., Sit W.H. and Lo S.C.L., Proteomic study of a model causative agent of harmful algal blooms, Prorocentrum triestinum II: The use of differentially expressed protein profiles under different growth phases and growth conditions for bloom prediction, Proteomics. 2004, 4: 3214-3226.

Chan L.L., Sit W.H., Hodgkiss I.J., Hsieh D.P. and Wan J.M.F., The use of species-specific and strain-specific peptides for the identification of PST-producing strains of Alexandrium minutum and A, tamarense, Congress Proteomics for Health, Switzerland. P.79. 2004.

Chan L.L., Hodgkiss I.J., Lam P.K.S., Wan J.M.F., Chou H.N., Lum J.H.K., Lo G.Y., Mak A.S.C., Sit W.H. and Lo S.C.L., Use of two-dimensional gel electrophoresis to differentiate morphospecies of Alexandrium minutum, a paralytic shellfish poisoning toxin-producing dinoflagellate of harmful algal blooms, Proteomics. 2005, 5: 1580-1593.

Hui P.Y., Sit W.H. and Wan J.M.F., Induction of S phase cell arrest and caspase activation by polysaccharide peptide isolated from Coriolus versicolor enhanced the cell cycle dependent activity and apoptotic cell death of doxorubicin and etoposide, but not cytarabine in HL-60 cells, Oncology Reports. 2005, 14: 145-155.

Ngai H.H.Y., Sit W.H. and Wan J.M.F., The Nephroprotective Effects of the Herbal Medicine Preparation, WH30+, on the Chemical-Induced Acute and Chronic Renal Failure in Rats, The American Journal of Chinese Medicine. 2005, 33: 491-500.

Wan J.M.F. and Sit W.H., Activation of caspase is independent to cyclin E expression in camptothecin-induced apoptosis in HL-60 cells, Cytometry 59A (1): 117 XXII ISAC congress, France. 2004.

Yang X., Sit W.H. and Wan J.M.F., Bivariate flow cytometric analysis of HL-60 cell cycle progression affected by polysaccharopeptide (PSP) from coriolus versicolor mycelia, Cytometry 59A (1): 118-119, XXII ISAC congress, France. 2004.

Yang X., Sit W.H., Chan D.K.O. and Wan J.M.F., The cell death process of the anticancer agent polysaccharide-peptide (PSP) in human promyelocytic leukemic HL-60 cells, Oncology Reports. 2005, 13: 1201-1210.

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Wang X

List of Research Outputs

Huo L., Fu G., Wang X., Ko K.W. and Wong A.O.L., Modulation of Calmodulin Gene Expression as a Novel Mechanism for Growth Hormone Feedback Control by Insulin-like Growth Factor in Grass Carp Pituitary Cells, Endocrinology. 2005, 146: 3821-3835.

Wang X. and Wong A.O.L., Program and Abstracts, 86th Annual Meeting of the Endocrine Society. Abstract P3-48, pp. 476, Adrenergic alpha 2 inhibition of growth hormone gene expression in Chinese grass carp pituitary cells.. 2004.

Researcher : Wang Y

List of Research Outputs

Li J., Wang Y. and Leung F.C.C., Cloning of chicken growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) and characterization of their spatial and temporal expression, Proceedings 15th International Congress Comparative Endocrinology, Boston, MA. p. 92. 2005.

Wang Y., Li J. and Leung F.C.C., Chicken epidermal growth factor (EGF): cDNA cloning, genomic organization and characterization of its expression in the ovary, Proceedings 15th International Congress Comparative Endocrinology, Boston, MA. p.133. 2005.

Wang Y. and Leung F.C.C., DNA structure constraint is probably a fundamental factor inducing CpG deficiency in bacteria , Bioinformatic. 2004, 20: 3336-3345.

Researcher : Wang Y

List of Research Outputs

Wang Y. and Leung F.C.C., DNA structure constraint is probably a fundamental factor inducing CpG deficiency in bacteria , Bioinformatic. 2004, 20: 3336-3345.

Researcher : Wong AOL

Project Title:	Novel aspects of adrenergic regulation of growth hormone release in fish
Investigator(s):	Wong AOL
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2002
Completion Date:	12/2004

Abstract:

To elucidate the neuroendocrine control and signal transduction mechanisms regulating growth hormone(GH) secretion in fish; to characterize the a2 adrenoreceptor subtype(s) expressed in goldfish somatotrophs; to elucidate the signaling pathways responsible for adrenergic regulation of GH release in goldfish pituitary cells.

Project Title:	Regulation of growth hormone synthesis in fish: mechanisms of action of the novel hypophysiotropic factor pituitary adenylate cyclase-activating polypeptide
Investigator(s):	Wong AOL
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2002

Abstract:

The project aims at understanding the mechanisms and evolution of neuroendocrine control of body growth in lower vertebrates. The research on GH synthesis and secretion of vertebrate endocrine will also provide new insights on the role of hormone biosynthesis in "stimulus-secretion" coupling of vertebrate endocrine systems.

Project Title:	Novel aspects of growth hormone autoregulation in grass carp pituitary cells:- intrapituitary "ultra-short" feedback on growth hormone gene expression in fish?
Investigator(s):	Wong AOL
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

To establish the structural identity of grass carp GH receptor; to provide a full-length receptor cDNA for subsequent functional expression studies; to confirm the expression of GH receptors in immuno-identified grass carp somatotrophs; to characterize ligand-binding properties of grass carp GH receptors expressed in CHO cells; to show the functional coupling of grass carp GH receptors with Ca²⁺i and Spi 2.1 gene activation; to examine the role of GH on GH synthesis and GH mRNA expression in grass carp pituitary cells; to examine the role of endogenous GH on GH mRNA expression induced by GH-releasing factors; to examine the effects of inhibiting the JAK/MARK cascade in GH-induced GH mRNA expression; to examine the effects of inhibiting the JAK/IRS/P13K cascade in GH-induced GH mRNA expression; to demonstrate GH modulation of GH transcript stability by altering the half-life (T1/2) of GHmRNA; to show that GH is essential to maintain basal expression of GH primary transcripts in the nucleus; to test if GH can alter the 5' promoter activity of grass carp GH gene using a reporter gene assay.

Project Title:	Novel aspects of growth hormone autoregulation in grass carp pituitary cells:- intrapituitary "ultra-short" feedback on growth hormone gene expression in fish?
Investigator(s):	Wong AOL
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

Project Title:	Paracrine regulation of growth hormone gene expression by gonadotropin secretion in carp pituitary cells
Investigator(s):	Wong AOL
Department:	Zoology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2004
Completion Date:	01/2005

Abstract:

To demonstrate the expression of GTH-II/LH receptors in immuno-identified carp somatotrophs; to examine the role of GTH-II in regulating the expression of GH primary transcripts in carp pituitary cells; to map the coarse location of "responsive sequence(s)" in the 5' promoter of grass carp GH gene mediating GTH-II induction of GH gene transcription.

Project Title:	Intrapituitary feedback loop regulating growth hormone synthesis:- paracrine regulation of growth hormone gene expression by gonadotropin release in grass carp pituitary cells
Investigator(s):	Wong AOL
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To study: (1) molecular cloning of grass carp GTH-II receptor by 3' and 5' RACE; (2) tissue distribution of GTH-II receptor transcripts in Chinese grass carp; (3) functional expression and characterization of grass carp GTH-II receptors; (4) GH mRNA expression regulated by interactions between GTH-II and GH regulators; (5) post-receptor signaling mechanisms mediating GTH-induced GH gene expression in grass carp pituitary cells; (6) functional role of GH mRNA stability and GH primary transcript in GTH-induced GH gene expression in grass carp pituitary cells; modulation of the 5' promoter activity of grass carp GH gene by GTH-II through the AC/cAMP/PKA/CREB pathway.

Project Title:	Intrapituitary feedback loop regulating growth hormone synthesis:- paracrine regulation of growth hormone gene expression by gonadotropin release in grass carp pituitary cells
Investigator(s):	Wong AOL
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Fung S.K. and Wong A.O.L., Grass carp activin: Molecular cloning and functional role in regulating growth hormone gene expression in grass carp pituitary cells, Program and Abstracts, 86th Annual Meeting of the Endocrine Society, Abstract P1-262, pp. 217. 2004.

Huo L., Lee K.Y., Leung P.C. and Wong A.O.L., Goldfish Calmodulin: Molecular Cloning, Tissue Distribution, and Regulation of Transcript Expression in Goldfish Pituitary Cells, Endocrinology. 2004, 145: 5056-5067.

Huo L., Fu G., Wang X., Ko K.W. and Wong A.O.L., Modulation of Calmodulin Gene Expression as a Novel Mechanism for Growth Hormone Feedback Control by Insulin-like Growth Factor in Grass Carp Pituitary Cells, Endocrinology. 2005, 146: 3821-3835.

Li W.S., Chen D., Wong A.O.L. and Lin H.R., Molecular cloning, tissue distribution, and ontogeny of mRNA expression of growth hormone in orange-spotted grouper (Epinephelus coioides), General and Comparative Endocrinology. 2005, 144: 78-89.

Wang X. and Wong A.O.L., Program and Abstracts, 86th Annual Meeting of the Endocrine Society. Abstract P3-48, pp. 476, Adrenergic alpha 2 inhibition of growth hormone gene expression in Chinese grass carp pituitary cells.. 2004.

Yip H.K.F., Lau W.M., Tsao G.S.W., So K.F. and Wong A.O.L., Zebrafish telomerase catalytic subunit: Molecular cloning and characterization of a ribonucleoprotein reverse transcriptase gene highly expressed in the retina, Association for Research in Vision and Ophthalmology Annual Meeting. 2005.

Zhou H., Jiang Y., Ko K.W., Li W. and Wong A.O.L., Paracrine regulation of growth hormone gene expression by gonadotrophin release in grass carp pituitary cells: functonal implications, molecular mechanisms and signal transduction, Journal of Molecular Endocrinology. 2005, 34: 415-432.

Researcher : Wong AST

Project Title:	HGF-Met and Wnt-[beta]-catenin signaling in human ovarian biology and cancer development
Investigator(s):	Wong AST
Department:	Zoology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To test whether constitutively active HGF/Met signaling plays a causative role in the development of ovarian cancer; to investigate the role of HGF in OSE, and uncover the signal transduction mechanism underlying its function; to elucidate the role of [beta]-catenin in ovarian cell physiology, and determine how it is regulated by reproductive endocrine factors.

Project Title:	The role of hepatocyte growth factor (HGF) in ovarian carcinoma cell migration/invasion
Investigator(s):	Wong AST
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2004

Abstract:

To study the downstream signaling pathways mediated by HGF for the invasive growth in human ovarian tumours.

Project Title:	The role of GRP/BiP and MnSOD in BRCA1-mediated breast/ovarian tumor suppression
Investigator(s):	Wong AST, He Q
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2004

Abstract:

To elucidate the role of GRP78/BiP and MnSOD in BRCA1-mediated breast and ovarian tumor suppression; to define the direct effect of BRCA1 on the altered expressions of GRP78/BiP and MnSOD by transfection and expression of functional BRCA1 gene in OSE cells; to examine the role of GRP78/BiP and MnSOD overespression in the regulation of apoptosis, cell growth and cell cycle progression, and in protecting cells from external stresses and resistance to cytotoxic drugs in breast and ovarian epithelial cells; to determine the mechanism (signal pathway) by which BRCA1 alters the gene activities of GRP78/BiP and MnSOD.

Project Title:	Synergistic action of epidermal growth factor and Met signalling pathways in the motile and invasive phenotype of ovarian cancer
Investigator(s):	Wong AST
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To study the downstream signalling pathways mediated by the cross-talk between EGF and HGF for the invasive growth in human ovarian tumours.

Project Title:	The role of GRP/BiP and MnSOD in BRCA1-mediated breast/ovarian tumor suppression
Investigator(s):	Wong AST
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

He Q.Y., Zhou Y., Wong E.S.Y., Ehlen T.G., Auersperg N., Chiu J. and Wong A.S.T., Proteomic analysis of a preneoplastic phenotype in ovarian surface epithelial cells derived from prophylactic oophorectomies, Gynecologic Oncology. 2005, 98: 68-76.

He Q.Y., Zhou Y., Wong E.S.Y., Ehlen T., Auersperg N., Chiu J. and Wong A.S.T., Proteomic analysis of human ovarian surface epithelial cells with germline mutations of BRCA1, Proceedings Society of Endocrinology, Metabolism and Reproduction 16, P-S4. 2004.

Pon Y.L., Auersperg N. and Wong A.S.T., Gonadotropins Regulate N-cadherin-mediated Human Ovarian Surface Epithelial Cell Survival at Both Post-translational and Transcriptional Levels through a Cyclic AMP/Protein Kinase A Pathway, The Journal of Biological Chemistry. 2005, 280: 15438-15448.

Pon Y.L., Auersperg N. and Wong A.S.T., Gonadotropins in the regulation of cadherin-mediated survival signals of ovarian surface epithelium, Proceedings Nature Biotechnology. 2005, 16: 134.

Pon Y.L. and Wong A.S.T., Gonadotropins: regulators of N-cadherin adhesion and inhibitors of cell survival in human ovarian surface epithelium, Proceedings Society of Endocrinology, Metabolism and Reproduction 16,S1. 2004.

Zhou H., Roskelley C.D., Pelech S., Miller D., Leung P.C.K., Auersperg N. and Wong A.S.T., ERK1/2, PI3K and p70S6K are important mediators for HGF-induced migration and invasion in human ovarian cancer cells, Proceedings Nature Biotechnology. 2005, 16: 94.

Zhou H., Keung Y.K. and Wong A.S.T., High levels of Met expression in the control of hepatocyte growth factor-dependent invasive growth in ovarian cancer cells, Proceedings Society of Endocrinology, Metabolism and Reproduction 16, P-S3. 2004.

Researcher : Wong CH

List of Research Outputs

Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-Germ Cell Anchoring Junction Dynamics in the Testis Are Regulated by an Interplay of Lipid and Protein Kinases, The Journal of Biological Chemistry. 2005, 280: 25029-25047.

Siu K.Y., Wong C.H., Lee W.W.M. and Cheng C.Y., Sertoli-germ cell anchoring junction dynamics in the testis are regulated by an interplay of lipid and protein kinases, Symposium on Post-meiotic Approaches in Male Contraception, June 6-8 2005, CUHK, Hong Kong. 2005.

Wong C.H., Mruk D.D., Siu K.Y., Lee W.W.M. and Cheng C.Y., Cell-cell junction dynamics at the blood-testis barrier (BTB) are regulated by a2-macroglobulin via the c-Jun N-terminal kinase (JNK) signaling pathway, The 44th American Society for Cell Biology Annual Meeting, Dec 4-8 2004, Washington, DC, USA. 2004.

Wong C.H., Xia W., Lee P.Y., Mruk D.D., Lee W.W.M. and Cheng C.Y., Regulation of Ectoplasmic Specialization Dynamics in the Seminiferous Epithelium by Focal Adhesion-Associated Proteins in Testosterone-Suppressed Rat Testes, Endocrinology. 2005, 146: 1192-1204.

Zhang J., Wong C.H., Xia W., Mruk D.D., Lee P.Y., Lee W.W.M. and Cheng C.Y., Regulation of Sertoli-Germ Cell Adherens Junction Dynamics via Changes in Protein-Protein Interactions of the N-Cadherin-b-Catenin Protein Complex which Are Possibly Mediated by c-Src and Myotubularin-Related Protein 2: An in Vivo Study Using an Androgen Suppression Model, Endocrinology. 2005, 146: 1268-1284.

Researcher : Wong ESY

List of Research Outputs

He Q.Y., Zhou Y., Wong E.S.Y., Ehlen T.G., Auersperg N., Chiu J. and Wong A.S.T., Proteomic analysis of a preneoplastic phenotype in ovarian surface epithelial cells derived from prophylactic oophorectomies, Gynecologic Oncology. 2005, 98: 68-76.

He Q.Y., Zhou Y., Wong E.S.Y., Ehlen T., Auersperg N., Chiu J. and Wong A.S.T., Proteomic analysis of human ovarian surface epithelial cells with germline mutations of BRCA1, Proceedings Society of Endocrinology, Metabolism and Reproduction 16, P-S4. 2004.

Researcher : Wong YL

List of Research Outputs

Wong Y.L., Wu H.Z. and Leung F.C.C., Molecular characterization of the Chinese isolates of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and the construction and characterization of the DNA vaccines, Journal of Animal and Veterinary Advances. 2005, 4: 63-70.

Researcher : Xia W

List of Research Outputs

Wong C.H., Xia W., Lee P.Y., Mruk D.D., Lee W.W.M. and Cheng C.Y., Regulation of Ectoplasmic Specialization Dynamics in the Seminiferous Epithelium by Focal Adhesion-Associated Proteins in Testosterone-Suppressed Rat Testes, Endocrinology. 2005, 146: 1192-1204.

Zhang J., Wong C.H., Xia W., Mruk D.D., Lee P.Y., Lee W.W.M. and Cheng C.Y., Regulation of Sertoli-Germ Cell Adherens Junction Dynamics via Changes in Protein-Protein Interactions of the N-Cadherin-b-Catenin Protein Complex which Are Possibly Mediated by c-Src and Myotubularin-Related Protein 2: An in Vivo Study Using an Androgen Suppression Model, Endocrinology. 2005, 146: 1268-1284.

Researcher : Xu RJ

Project Title:	A study on the physiological role of transforming growth factor [beta] in postnatal adaptation of the gastrointestinal tract in neonatal pigs
Investigator(s):	Xu RJ
Department:	Zoology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To investigate the possible physiological role of TGF-[beta] in regulation of postnatal adaptation of the gastrointestinal tract in neonatal animals.

Project Title:	Postnatal adaptation of the gastrointestinal tract in neonatal pigs: a possible role of transforming growth factor-[beta]
Investigator(s):	Xu RJ
Department:	Zoology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

To examine concentration changes of TGF-[beta] in milk during the lactation; to study chemical properties of TGF-[beta] in milk; to examine the stability of milk-borne TGF-[beta] in the GI lumen; to study the development changes of endogenous production of TGF-[beta] in the GI tract; to study the effects of milk on intestinal mutation in newborn piglets; to study the endogenous production of TGF-D and its receptors in the gastrointestinal tract in early-weaned piglets.

Project Title:	Postnatal adaptation of the gastrointestinal tract in neonatal pigs: a possible role of transforming growth factor-[beta]
Investigator(s):	Xu RJ
Department:	Zoology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

List of Research Outputs

Chen C.Y., Wang T., Huo Y.J., Xu R.J. and Zhou G.L., Biochemical analysis of hepatic cell development in newborn pigs, Veterinary and Animal Science (Chinese). 2005, 36, issue 2: 153-157.

Lin G.J., Wang T. and Xu R.J., Metabolic effects of hydrolysed casein in newborn IUGR pigs, Proceedings of 9th Scientific Meeting of Chinese Veterinary and Animal Science Association, p. 67. 2004.

Lin G.J., Wang T. and Xu R.J., Metabolic effects of oral ingestion of insulin in newborn pigs, Proceedings of 9th Scientific Meeting of Chinese Veterinary and Animal Science Association, p. 88. 2004.

Mei J. and Xu R.J., Transient changes of transforming growth factor-b expression in the small intestine of the pig in association with weaning, British Journal of Nutrition. 2005, 93: 37-45.

Sangild P.T. and Xu R.J., Colostrum, Encyclopedia of Animal Science. Marcel Dekker, New York, 2004.

Wang T., Huo Y.J., Shi F.X., Xu R.J. and Hutz R.J., Effects of Intrauterine Growth Retardation on Development of the Gastrointestinal Tract in Neonatal Pigs, Biology of the Neonate. 2005, 88: 66-72.

Wang T., Huo Y.J., Xu R.J. and Chen J., Influences of intrauterine growth retardation and oral insulin on the expressions of some genes in the intestinal mucosa of neonatal pigs. The proceeding of 2004 International Symposium on Feed Additive, Animal Nutrition and Health (July 2004, Guangzhou, China), p.68-81. 2004.

Wang T., Xu R.J. and Huo Y.J., Oral insulin stimulates intestinal development and enzyme maturation in newborn pigs, Agricultural Sciences in China. 2004, 3, No. 9: 707-715.

Zhang Y.Q., He X.Z., Zhang J.S., Wang R.A., Zhou J. and Xu R.J., Stage-specific localization of transforming growth factor beta 1 and beta 3 and their receptors during spermatogenesis in men, Asian Journal of Andrology. 2004, 6(2): 105-109.

Researcher : Yang X

List of Research Outputs

Yang X., Sit W.H. and Wan J.M.F., Bivariate flow cytometric analysis of HL-60 cell cycle progression affected by polysaccharopeptide (PSP) from coriolus versicolor mycelia, Cytometry 59A (1): 118-119, XXII ISAC congress, France. 2004.

Yang X., Sit W.H., Chan D.K.O. and Wan J.M.F., The cell death process of the anticancer agent polysaccharide-peptide (PSP) in human promyelocytic leukemic HL-60 cells, Oncology Reports. 2005, 13: 1201-1210.

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Yip CW

List of Research Outputs

Yip C.W., Hon C.C., Zeng F., Chow Y.C.K. and Leung F.C.C., Prevalence of non-pneumonic infections with SARS-correlated virus, Lancet. 2004, 363: 1826-1827.

Researcher : Zeng F

List of Research Outputs

Yip C.W., Hon C.C., Zeng F., Chow Y.C.K. and Leung F.C.C., Prevalence of non-pneumonic infections with SARS-correlated virus, Lancet. 2004, 363: 1826-1827.

Zeng F., Hon C.C., Sit W.H., Chow Y.C.K., Hui K.H., Law K.M., Ng W.L.V., Yang X., Leung F.C.C. and Wan J.M.F., Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray, International Journal of Oncology. 2005, 27: 513-523.

Researcher : Zhang J

List of Research Outputs

Zhang J., Wong C.H., Xia W., Mruk D.D., Lee P.Y., Lee W.W.M. and Cheng C.Y., Regulation of Sertoli-Germ Cell Adherens Junction Dynamics via Changes in Protein-Protein Interactions of the N-Cadherin-b-Catenin Protein Complex which Are Possibly Mediated by c-Src and Myotubularin-Related Protein 2: An in Vivo Study Using an Androgen Suppression Model, Endocrinology. 2005, 146: 1268-1284.

Researcher : Zhou H

List of Research Outputs

Zhou H., Roskelley C.D., Pelech S., Miller D., Leung P.C.K., Auersperg N. and Wong A.S.T., ERK1/2, PI3K and p70S6K are important mediators for HGF-induced migration and invasion in human ovarian cancer cells, Proceedings Nature Biotechnology. 2005, 16: 94.

Zhou H., Keung Y.K. and Wong A.S.T., High levels of Met expression in the control of hepatocyte growth factor-dependent invasive growth in ovarian cancer cells, Proceedings Society of Endocrinology, Metabolism and Reproduction 16, P-S3. 2004.

Zhou H., Jiang Y., Ko K.W., Li W. and Wong A.O.L., Paracrine regulation of growth hormone gene expression by gonadotrophin release in grass carp pituitary cells: functonal implications, molecular mechanisms and signal transduction, Journal of Molecular Endocrinology. 2005, 34: 415-432.

Researcher : Zhou H

List of Research Outputs

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