Dept of Microbiology

DEPARTMENT OF MICROBIOLOGY

Researcher : Altmeyer RM

List of Research Outputs

Lozach P...Y..., Amara A..., Bartosch B., Virelizier J...L..., Arenzana-Seisdedos F..., Cosset F...L... and Altmeyer R.M., C-type Lectins L-SIGN and DC-SIGN Capture and Transmit Infectious Hepatitis C Virus Pseudotype Particles, Journal of Biological Chemistry. 2004, 279: 32035-32045.

Zhang X., Yap Y.L. and Altmeyer R.M., Generation of predictive pharmacophore model for SARS-Coronavirus main proteinase, European Journal of Medicinal Chemistry. 2004, 40: 57-62.

Researcher : Cai J

List of Research Outputs

Cai J., Smith D.K., Xia X. and Yuen K.Y., MBEToolbox: a MATLAB toolbox for sequence data analysis in molecular biology and evolution., BMC bioinformatics. 2005, 6(1): 64.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Researcher : Cai J

List of Research Outputs

Cai J., Smith D.K., Xia X. and Yuen K.Y., MBEToolbox: a MATLAB toolbox for sequence data analysis in molecular biology and evolution., BMC bioinformatics. 2005, 6(1): 64.

Researcher : Cao K

List of Research Outputs

Cao K., Zheng Y., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H. and Zheng B., Peripheral NK Cells Play a Complementary Role for Anti-tumor Immunity in Nasopharyngeal Carcinoma Patients., Keystone Symposia – Basic Aspects of Tumor Immunology II (C3). Keystone, Colorado, USA.. 2005.

Researcher : Cao L

Project Title:	Identification and utilization of tumor specific serological markers for the early detection of esophageal cancer
Investigator(s):	Cao L, Ng MH
Department:	Microbiology
Source(s) of Funding:	Other Funding Scheme
Start Date:	07/1995

Abstract:

It is estimated that 50,000 people develop esophageal cancer each year in the developed countries, and at least 10 times of that number in developing countries, especially in China. Only about 10% of these patients recover from the disease after treatment. This is largely because early symptoms of the cancer is innocuous, and therefore it is not diagnosed until the disease is at an advanced stage. The proposal aims to develop a test for early diagnosis of this cancer in order to improve patients' chance of recovery. The project will be carried out in 3 stages. The first is to identify genes that are specifically expressed in the cancer at high levels. The second is to develop tests to detect the products encoded by these genes. The third is to carry out clinical trails to determine which of these tests can be used for early diagnosis with adequate sensitive and specificity.

List of Research Outputs

Researcher : Chan CS

List of Research Outputs

Yuen R.M.F., Wong D.K.H., Chan C.S., Zhang Z., Yuen J.C.H. and Lai C.L., Immune Response During Immunoclearance Phase of Chinese Patients with HBV Genotypes B and C, Journal of Hepatology. 2005, 42 (Suppl 2): 152.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Researcher : Chan KCW

List of Research Outputs

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zheng B., Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ma S. and Ng M.H., Effects of single nucleotide polymorphism in the RANTES promoter region on HIV infection and disease progression in indigenous Chinese, XV international AIDS conference. 11-16 July, 2004. Bangkok, Thailand.. 2004.

Researcher : Chan KH

List of Research Outputs

Chan K.H., Yau C.Y., Chu K.W., Poon L.L.M., Maldeis N.E. and Peiris J.S.M., Evaluation of Reverse Transcriptase-Strand Displacement Amplification (BD Probetec^TM) for Rapid Diagnosis of SARS Coronavirus Infection, 21st Annual Clinical Virology Symposium; Florida, USA; 8-9 May, 2005.. 2005.

Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75.

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Chiang A.K.S., Chan C.W., Lee P.P.W. and Chan K.H., Epstein-Barr Virus-associated Infectious Mononucleosis in Chinese Children, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 46.

Chiang A.K.S., Chan C.W., Chan K.H. and Lau A.S.Y., Epstein-barr Virus-associated Infectious Mononucleosis in Chinese Children, 5th Guangdong - Hong Kong Paediatric Exchange Meeting, HK, 24 July 2004.

Chiang A.K.S., Lam S.Y. and Chan K.H., Kinetics of EBV-specific Antibody in Symptomatic and Asymptomatic Primary Epstein-Barr Virus Infection in Chinese Children, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 46.

Chiang A.K.S., Chan G.C.F., Ha S.Y. and Chan K.H., Respiratory Syncytial Virus (RSV) infections in immunocompromized children, American Society of Haematoogy, 46th Annual Meeting and Exposition, San Diego, California, USA, 4-7 December 2004. 410b.

Chiu S.S.S., Chan K.H., Chu K.W., Kwan S.W., Guan Y., Poon L.L.M. and Peiris J.S.M., Human Coronavirus NL63 Infection and Other Coronavirus Infections in Children Hospitalized with Acute Respiratory Disease in Hong Kong, China, Clinical Infectious Diseases. 2005, 40: 172-11729.

Chu C.M., Leung W.S., Cheng V.C.C., Chan K.H., Lin A.W., Chan V.L., Lam J.Y., Chan K.S. and Yuen K.Y., Duration of RT-PCR positivity in severe acute respiratory syndrome., The European respiratory journal. 2005, 25(1): 12-4.

Chu C.M., Poon L.L.M., Cheng V.C.C., Chan K.S., Hung I.F., Wong M.M., Chan K.H., Leung W.S., Tang S.F., Chan V.L., Ng W.L., Sim T.C., Ng P.W., Law K.I., Tse D.M., Peiris J.S.M. and Yuen K.Y., Initial viral load and the outcomes of SARS., Canadian Medical Association journal . 2004, 171(11): 1349-52.

Fan H., Nicholls J.M., Chua D.T.T., Chan K.H., Sham J.S.T., Lee S. and Gulley M.L., Laboratory Markers Of Tumor Burden In Nasopharyngeal Carcinoma: A Comparison Of Viral Load And Serologic Tests For Epstein-barr Virus, International Journal of Cancer. 2004, 112(6): 1036-41.

Guan M., Chan K.H., Peiris J.S.M., Kwan S.W., Lam S.Y., Pang C.M., Chu K.W., Chan K.M., Chen H.Y., Phuah E.B. and Wong C.J., Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome., Clinical and Diagnostic Laboratory Immunology. 2004, 11(4): 699-703.

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Ip E.W.K., Chan K.H., Law H.K.W., Tso H.W., Kong K.P., Wong W.H.S., To Y.F., Yung W.W.H., Chow E.Y., Au K.L., Chan E.Y.T., Lim W., Jensenius J.C., Turner M.W., Peiris J.S.M. and Lau Y.L., Mannose-Binding Lectin in Severe Acute Respiratory Syndrome Coronavirus Infection, Journal of Infectious Diseases. 2005, 191: 1697-1704.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Leung A.Y.H., Chan M.T.L., Yuen K.Y., Cheng V.C.C., Chan K.H., Wong L.P., Liang R.H.S., Lie A.K.W. and Kwong Y.L., Ciprofloxacin Decreased Polyoma BK Virus Load in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation, Clinical Infectious Disease. 2005, 40: 528-537.

Poon L.L.M., Wong B.W., Chan K.H., Leung C.S., Yuen K.Y., Guan Y. and Peiris J.S.M., A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors., Journal of clinical virology. 2004, 30(3): 214-7.

Poon L.L.M., Leung C.S.W., Chan K.H., Lee H.C., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of human influenza A viruses by loop-mediated isothermal amplification., Journal of Clinical Microbiology. 2005, 43(1): 427-30.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Chan K.H., Hui W.T., Kwan G.S., Peiris J.S.M., Couch R.B. and Yuen K.Y., False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide., Journal of clinical microbiology. 2004, 42(12): 5885-8.

Woo P.C.Y., Lau S.K.P., Wong B.H., Chan K.H., Chu C.M., Tsoi H.W., Huang Y., Peiris J.S.M. and Yuen K.Y., Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus, Clinical and diagnostic laboratory immunology. 2004, 11(4): 665-8.

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Researcher : Chan KM

List of Research Outputs

Guan M., Chan K.H., Peiris J.S.M., Kwan S.W., Lam S.Y., Pang C.M., Chu K.W., Chan K.M., Chen H.Y., Phuah E.B. and Wong C.J., Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome., Clinical and Diagnostic Laboratory Immunology. 2004, 11(4): 699-703.

Lau S.K.P., Woo P.C.Y., Fung A.M., Chan K.M., Woo G.K.S. and Yuen K.Y., Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing., Journal of medical microbiology. 2004, 53(Pt 12): 1247-53.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M., Tang S.F. and Yuen K.Y., Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing., Journal of clinical pathology. 2005, 58(3): 301-7.

Researcher : Chan KM

List of Research Outputs

Researcher : Chan KS

List of Research Outputs

Chu C.M., Leung W.S., Cheng V.C.C., Chan K.H., Lin A.W., Chan V.L., Lam J.Y., Chan K.S. and Yuen K.Y., Duration of RT-PCR positivity in severe acute respiratory syndrome., The European respiratory journal. 2005, 25(1): 12-4.

Chu C.M., Poon L.L.M., Cheng V.C.C., Chan K.S., Hung I.F., Wong M.M., Chan K.H., Leung W.S., Tang S.F., Chan V.L., Ng W.L., Sim T.C., Ng P.W., Law K.I., Tse D.M., Peiris J.S.M. and Yuen K.Y., Initial viral load and the outcomes of SARS., Canadian Medical Association journal . 2004, 171(11): 1349-52.

Yap F.H.Y., Ho P.L., Lam K.F., Chan K.S., Cheng Y.H. and Peiris J.S.M., Excess hospital admissions for pneumonia, chronic obstructive pulmonary diseases, and heart failure during influenza seasons in Hong Kong, Journal of Medical Virology. Wiley-Liss, Inc., 2004, 73: 617-623.

Researcher : Chan MCW

Project Title:	Establishment of primary culture of human airway epithelial cells, as an in-vitro model to study human infection of coronavirus/avian influenza virus
Investigator(s):	Chan MCW, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To establish a primary culture of human airway and alveolar epithelial cells, which can act as an in-vitro model to study human infection of coronavirus and avian influenza virus.

Researcher : Chan YO

List of Research Outputs

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells, Blood. 2005, 106(7): 2366-2374.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine upregulation in SARS coronavirus infected human monocyte derived dendritic cells, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 85.

Researcher : Che X

List of Research Outputs

Che X., Hao W., Wang Y., Di B., Yin K., Xu Y.C., Feng C.S., Wan Z.Y., Cheng V.C.C. and Yuen K.Y., Nucleocapsid protein as early diagnostic marker for SARS., Emerging infectious diseases. 2004, 10(11): 1947-9.

Researcher : Chen H

Project Title:	Modification of cells for isolation and characterization of SARS CoV from animal sources
Investigator(s):	Chen H
Department:	Microbiology
Source(s) of Funding:	Seed Funding for New Staff
Start Date:	07/2004

Abstract:

To provide: (1) a panel of cell lines (pools) for the isolation of SARS CoV from animal sources; (2) information on molecular mechanism of interspecies transmission of SARS CoV; (3) clues for the further molecular virology studies on this virus.

Project Title:	Biological function/relevance for the 29 nucleotides insertion in the genome of animal SARS like CoV
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

This study will serve as a pilot investigating the potential effect of insertion/deletion on expression of viral proteins down stream of this region and provide clues for further investigation into the molecular mechanism for SARS coronavirus interspecies transmission.

List of Research Outputs

Chen H., Huang J., Wu Y., Liao G., Hutt-Fletcher L. and Hayward S.D., Chen H, Huang J, Wu FY, Liao G, Hutt-Fletcher L, Hayward SD. Related Articles, Links Regulation of expression of the Epstein-Barr virus BamHI-A rightward transcripts., Journal of Virology. 2005, 79(3): 1724-33.

Cheung K.W., Guan Y., Ng S.F., Chen H., Wong C.H.K., Peiris J.S.M. and Poon L.L.M., Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron., Journal of General Virology. 2005, 86(Pt 5): 1447-54.

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Chen Y

List of Research Outputs

He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Chen Y

List of Research Outputs

Researcher : Cheng SLE

List of Research Outputs

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distribution and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong children., Vaccine. 2004, 22(25-26): 3334-9.

Researcher : Cheng VCC

List of Research Outputs

Au W.Y., Hon C., Cheng V.C.C. and Ma E.S.K., Concomitant zoster myelitis and cerebral leukemia relapse after stem cell transplantation, Annals of Hematology. 2004, 84(1): 59-60.

Au W.Y., Lie A.K.W. and Cheng V.C.C., Hematopoietic progenitor cell transplantation from a donor with indeterminate human immunodeficiency virus antibody status, Transfusion. 2005, 45(5): 819-820.

Au W.Y., Cheng V.C.C., Wan T.S.K. and Ma E.S.K., Myelodysplasia masquerading as parvovirus-related red cell aplasia with giant pronormoblasts, Annals of Hematology. 2004, 83(10): 670-1.

Au W.Y., Lie A.K.W. and Cheng V.C.C., VDRL screening and haemopoietic stem cell transplantation, Bone Marrow Transplantation. 2005, 35: 1027-1028.

Che X., Hao W., Wang Y., Di B., Yin K., Xu Y.C., Feng C.S., Wan Z.Y., Cheng V.C.C. and Yuen K.Y., Nucleocapsid protein as early diagnostic marker for SARS., Emerging infectious diseases. 2004, 10(11): 1947-9.

Che X.Y., Qiu L.W., Liao Z.Y., Wang Y.D., Wen K., Pan Y.X., Hao W., Mei Y.B., Cheng V.C.C. and Yuen K.Y., Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43., The Journal of infectious diseases. 2005, 191(12): 2033-7.

Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75.

Cheng V.C.C., Tang S.F., Wu A.K., Chu C.M. and Yuen K.Y., Medical treatment of viral pneumonia including SARS in immunocompetent adult., The Journal of infection. 2004, 49(4): 262-73.

Chu C.M., Leung W.S., Cheng V.C.C., Chan K.H., Lin A.W., Chan V.L., Lam J.Y., Chan K.S. and Yuen K.Y., Duration of RT-PCR positivity in severe acute respiratory syndrome., The European respiratory journal. 2005, 25(1): 12-4.

Chu C.M., Poon L.L.M., Cheng V.C.C., Chan K.S., Hung I.F., Wong M.M., Chan K.H., Leung W.S., Tang S.F., Chan V.L., Ng W.L., Sim T.C., Ng P.W., Law K.I., Tse D.M., Peiris J.S.M. and Yuen K.Y., Initial viral load and the outcomes of SARS., Canadian Medical Association journal . 2004, 171(11): 1349-52.

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Leung A.Y.H., Chan M.T.L., Yuen K.Y., Cheng V.C.C., Chan K.H., Wong L.P., Liang R.H.S., Lie A.K.W. and Kwong Y.L., Ciprofloxacin Decreased Polyoma BK Virus Load in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation, Clinical Infectious Disease. 2005, 40: 528-537.

Leung A.Y.H., Chan M.T.L., Cheng V.C.C., Lie A.K.W., Yuen K.Y. and Kwong Y.L., Polyoma BK viruria in patients undergoing autologous hematopoietic stem cell transplantation, Bone Marrow Transplantation. 2005, 35: 1029−1030.

Leung A.Y.H., Chan M.T.L., Cheng V.C.C., Yuen K.Y. and Kwong Y.L., Quantification of Adenovirus in the Lower Respiratory Tract of Patients without Clinical Adenovirus-Related Respiratory Disease, Clinical Infectious Disease. 2005, 40: 1541-1544(online).

Ng K.H., Wu A.K., Cheng V.C.C., Tang S.F., Chan C.Y., Yung C.Y., Luk S.H., Lee T.W., Chow L. and Yuen K.Y., Pulmonary artery thrombosis in a patient with severe acute respiratory syndrome., Postgraduate medical journal. 2005, 81(956): e3.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Wu A.K., Cheng V.C.C., Tang S.F., Hung I.F., Lee R.A., Hui D.S. and Yuen K.Y., The unmasking of Pneumocystis jiroveci pneumonia during reversal of immunosuppression: case reports and literature review., BMC infectious diseases. 2004, 4(1): 57.

Researcher : Cheung CY

List of Research Outputs

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E. .W., Vogel L., Chu S.L., Chan C.M., Tse K.S., Subarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Immunization with trimeric S glycoprotein protects from SARS-CoV challenge despite the presence of in vitro facilitating antibodies, Xth International Nidovirus Symposium,Toward Control of SARS and Other Nidovirus Diseases. Colorado Springs, 2005.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells, Blood. 2005, 106(7): 2366-2374.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine upregulation in SARS coronavirus infected human monocyte derived dendritic cells, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 85.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Human Monocyte Derived Dendritic Cells Infected by SARS Associated Coronavirus, 12th International Congress of Immunology and 4th Annual Conference of FOCIS, Montreal, Canada, 18-23 July 2004.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Peiris J.S.M. and Lau Y.L., SARS Coronavirus Induced Chemokines Expression in Human Monocyte Derived Dendritic Cells, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 48.

Lee D.C.W., Li C.B., Cheung C.Y., Mok K.P., Law H.Y., Peiris J.S.M. and Lau A.S.Y., Immune Response to Virus Infections: Molecular Signaling Pathways of HIV - and H5N1-induced Cytokine Dysregulation in Disease Pathogenesis, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 13.

Mok K.P., Lee D.C.W., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., Cytokine dysregulation and differential onset of apoptosis in H5N1- and H1N1-infected human blood macrophages. , In: Mok CKP, Lee CW, Cheung CY, Peiris M and Lau ASY. , 9th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong. December 2004.. 2004.

Mok K.P., Lee D.C.W., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., Differential onset of apoptosis and its mechanisms in H5N1- and H1N1-infected human blood macrophages., In: Mok CKP, Lee CW, Cheung CY, Peiris M and Lau ASY. , 34th Annual Scientific Meeting of the Australasian Society for Immunology Australia. December 2004.. 2004.

Ren Y., He Q., Fan J.Q., Jones B., Zhou Y., Xie Y., Cheung C.Y., Wu A.Y.Y., Chiu J., Peiris J.S.M. and Tam P.K.H., The use of proteomics in the discovery of serum biomarkers from patients with severe acute respiratory syndrome, Proteomics. 2004, 4: 3477-3484.

Zhou J., Law H.K.W., Cheung C.Y., Peiris J.S.M. and Lau Y.L., Upregulation of Death Receptor Ligands in Adult and Neonatal Macrophages by Avian Influenza Viruses: A Mechanism for the Unusual Severity of Human Diseases?, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 52.

Zhou J., Law H.K.W., Cheung C.Y., Peiris J.S.M. and Lau Y.L., Upregulation of Death Receptor Ligands in Human Antigen Presenting Cells by Avian Flu Viruses: a Mechanism for the Unusual Severity of Human Diseases?, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 113.

Researcher : Cheung KW

List of Research Outputs

Cheung K.W., Guan Y., Ng S.F., Chen H., Wong C.H.K., Peiris J.S.M. and Poon L.L.M., Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron., Journal of General Virology. 2005, 86(Pt 5): 1447-54.

Researcher : Chong TK

List of Research Outputs

Chong T.K., Cloning and characterization of immunogenic proteinsof the antigenic mannoprotein superfamily in Aspergillus (PhD thesis), supervisor: Woo P.C.Y., Yuen K.Y., 2005.

Woo P.C.Y., Lau S.K.P., Lau C.C., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient., Bone marrow transplantation. 2005, 35(8): 831-3.

Researcher : Chow KH

List of Research Outputs

Ho P.L., Tse C.W., Mak C.K., Chow K.H. and Ng T.K., Community-acquired methicillin-resistant Staphylococcus aureus arrives in Hong Kong., Journal of Antimicrob Chemotherapy. 2004, 54(4): 845-6.

Ho P.L., Shek R.H., Chow K.H., Duan R., Mak C.K., Lai E.L.Y., Yam W.C., Tsang K.W.T. and Lai W.M., Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002., Jouranl of Antimicrobial Chemotherapy. 2005, 55(3): 326-32.

Ho P.L., Ho A.Y., Chow K.H., Wong R.C., Duan R.S., Ho W.L., Mak G.C., Tsang K.W., Yam W.C. and Yuen K.Y., Occurrence and molecular analysis of extended-spectrum {beta}-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002., The Journal of antimicrobial chemotherapy. 2005, 55(6): 840-5.

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distribution and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong children., Vaccine. 2004, 22(25-26): 3334-9.

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Researcher : Chow KH

List of Research Outputs

Ho P.L., Tse C.W., Mak C.K., Chow K.H. and Ng T.K., Community-acquired methicillin-resistant Staphylococcus aureus arrives in Hong Kong., Journal of Antimicrob Chemotherapy. 2004, 54(4): 845-6.

Researcher : Chu KW

List of Research Outputs

Chan K.H., Yau C.Y., Chu K.W., Poon L.L.M., Maldeis N.E. and Peiris J.S.M., Evaluation of Reverse Transcriptase-Strand Displacement Amplification (BD Probetec^TM) for Rapid Diagnosis of SARS Coronavirus Infection, 21st Annual Clinical Virology Symposium; Florida, USA; 8-9 May, 2005.. 2005.

Chiu S.S.S., Chan K.H., Chu K.W., Kwan S.W., Guan Y., Poon L.L.M. and Peiris J.S.M., Human Coronavirus NL63 Infection and Other Coronavirus Infections in Children Hospitalized with Acute Respiratory Disease in Hong Kong, China, Clinical Infectious Diseases. 2005, 40: 172-11729.

Guan M., Chan K.H., Peiris J.S.M., Kwan S.W., Lam S.Y., Pang C.M., Chu K.W., Chan K.M., Chen H.Y., Phuah E.B. and Wong C.J., Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome., Clinical and Diagnostic Laboratory Immunology. 2004, 11(4): 699-703.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Researcher : Du L

List of Research Outputs

Wang M., Gao Y., Zhou D.H., Wu X.W., Chen X.S., Di B., Liu Y.F., Chen F., Du L., Xu H.F., Gu J., Zheng B. and Xu J.G., The effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus., Zhonghua Liu Xing Bing Xue Za Zhi. 2005, 26: 22-24.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Duan L

List of Research Outputs

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Researcher : Duan R

List of Research Outputs

Ho P.L., Shek R.H., Chow K.H., Duan R., Mak C.K., Lai E.L.Y., Yam W.C., Tsang K.W.T. and Lai W.M., Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002., Jouranl of Antimicrobial Chemotherapy. 2005, 55(3): 326-32.

Researcher : Duan RS

List of Research Outputs

Ho P.L., Ho A.Y., Chow K.H., Wong R.C., Duan R.S., Ho W.L., Mak G.C., Tsang K.W., Yam W.C. and Yuen K.Y., Occurrence and molecular analysis of extended-spectrum {beta}-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002., The Journal of antimicrobial chemotherapy. 2005, 55(6): 840-5.

Researcher : Guan Y

Project Title:	Molecular evolution studies on H5N1/97 and related avian influenza viruses
Investigator(s):	Guan Y, Peiris JSM, Shortridge KF
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2001
Completion Date:	08/2004

Abstract:

To develop methods that can be applied to screen large numbers of virus isolates for genetic reassortants; to understand how the precursors of H5N1/97 virus evolved, and continue to evolve, in their hosts in southern China.

Project Title:	Vaccines for the control of H5N1 and other avian influenza viruses
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	The University of Hong Kong Foundation Seed Grant
Start Date:	04/2002

Abstract:

To study vaccines for the control of H5N1 and other avian influenza viruses.

Project Title:	Genetic determinants of influenza virus for inter-species transmission: studies using a reverse genetic approach
Investigator(s):	Guan Y, Peiris JSM, Poon LLM, Shortridge KF
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	08/2002

Abstract:

Avian influenza viruses can directly impact on human and animal health and thus cause economic loss. The study aims to establishing a reverse genetic system and to determine the biological significance of the deletion of NA stalk and the NS genes, particularly in relation to the host-restriction and pathogenesis. Results can enhance understanding of the machanism of inter-species transmission of influenza virus.

Project Title:	Searching for the precursor of SARS-Cov in Southern China
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To find source of SARS-Cov, and how this virus emerged in humans.

Project Title:	The role of migratory birds for evolution of influenza gene pool of southern China
Investigator(s):	Guan Y, Poon LLM, Li K.S.
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To observe the epidemiological dynamics of influenza viruses between domestic ducks (the major influenza gene reservoirs in this region) and migratory birds in southern China; to understand the mechanisms of inter-species transmission of influenza virus from wild aquatic birds to domestic poultry, or vice versa; to determine whether migratory birds may contribute some gene segments for the generation of influenza viruses of pandemic potential.

Project Title:	Identification of the possible animal reservoirs for SARS-like coronavirus in southern China
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

List of Research Outputs

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Cheung K.W., Guan Y., Ng S.F., Chen H., Wong C.H.K., Peiris J.S.M. and Poon L.L.M., Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron., Journal of General Virology. 2005, 86(Pt 5): 1447-54.

Chiu S.S.S., Chan K.H., Chu K.W., Kwan S.W., Guan Y., Poon L.L.M. and Peiris J.S.M., Human Coronavirus NL63 Infection and Other Coronavirus Infections in Children Hospitalized with Acute Respiratory Disease in Hong Kong, China, Clinical Infectious Diseases. 2005, 40: 172-11729.

Choi Y.K., Ozaki H., Webby R.J., Webster R.G., Peiris J.S.M., Poon L.L.M., Butt C., Leung C.Y.H. and Guan Y., Continuing evolution of H9N2 influenza viruses in Southeastern China., Journal of virology. 2004, 78(16): 8609-14.

Ellis T.M., Leung C.Y.H., Chow M.K., Bissett L.A., Wong W., Guan Y. and Peiris J.S.M., Vaccination of chickens against H5N1 avian influenza in the face of an outbreak interrupts virus transmission., Avian pathology. 2004, 2004: 405-12.

Govorkova E.A., Rehg J.E., Krauss S., Yen H.L., Guan Y., Peiris J.S.M., Nguyen T.D., Hanh T.H., Puthavathana P., Long H.T., Buranathai C., Lim W., Webster R.G. and Hoffmann E., Lethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004., Journal of Virology. 2005, 79(4): 2191-8.

Guan Y., "亞熱帶地區新發傳染病應對策略高峰論壇" organised by Guangxi Zhuang Autonomous Region Center for Disease Prevention and Control in Guangxi, China from 15 Jun – 18 Jun 2005. The title of my lecture is “The genesis and origin of H5N1 avian influenza viruses in eastern Asia”., 2005.

Guan Y., Global Vaccine Research Forum and Parallel HIV/Enteric Vaccine Symposia organised by WHO in Brazil from 12 Jun- 15 Jun 2005. The title of presentation is Genetic and antigenic difference of H5N1 influenza viruses isolated from Vietnam and Indonesia., 2005.

Guan Y., Infectious Diseases THREATENING Hong Kong and China: Challenges and Prevention Effectiveness organised by TheChinese University of Hong Kong in Hong Kong from 26 Feb - 27 Feb 2005. The title of my presentation is Characterization of a H9N2 influenza virus isolated from human in Hong Kong in 2003., 2005.

Guan Y., International Symposium on: Emergence and Control of Viral Respiratory Diseases organised by the MerieuxFoundation at Les Pensieres, Veyrier-du-Lac (French Alps) from 30 Nov - 3 Dec 2004. The title of my talk is Emergence and Re-emergence of H5N1 avian H5N1influenza viruses in poultry in eastern Asia., 2004.

Guan Y., WHO Animal Influenza Networkmeeting at Geneva, WHO/HQ on 15 Nov 2004. The title of my presentation is Continuing the endemicity of highly pathogenic H5N1 influenza virus in southern China., 2004.

Guan Y., “the Xth International Nidovirus Symposium: Toward Control of SARS and Other Nidovirus Diseases” organised by University of Colorado at Denver and Health Sciences Center at Colorado Springs, USA from 25 Jun – 30 Jun 2005. The title of my talk is “The role of wild animals from markets of southern China in mediating SARS-CoV to humans”. , 2005.

He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Li W.S., Zhang C., Sui J., Kuhn H., Moore M.J., Luo S., Wong S.K., Huang I.C., Xu K., Vasilieva N., Murakami A., He Y., Marasco W.A., Guan Y., Choe H. and Farzan M., Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. , The EMBO journal. 2005, 24(8): 1634-1643.

Lipatov A.S., Govorkova E.A., Webby R.J., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webster R.G., Influenza: emergence and control., Journal of virology. 2004, 2004: 8951-9.

Peiris J.S.M. and Guan Y., Confronting SARS: a view from Hong Kong., Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 2004, 359(1447): 1075-9.

Peiris J.S.M., Guan Y. and Yuen K.Y., Severe acute respiratory syndrome., Nature medicine. 2004, 10(12 Suppl): S88-97.

Peiris J.S.M., Guan Y. and Yuen K.Y., Special Research Achievement Award for the Research Team in the Department of Microbiology 2004., The University of Hong Kong. 2004.

Poon L.L.M., Wong B.W., Chan K.H., Leung C.S., Yuen K.Y., Guan Y. and Peiris J.S.M., A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors., Journal of clinical virology. 2004, 30(3): 214-7.

Poon L.L.M., Leung C.S.W., Chan K.H., Lee H.C., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of human influenza A viruses by loop-mediated isothermal amplification., Journal of Clinical Microbiology. 2005, 43(1): 427-30.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Poon L.L.M., Guan Y., Nicholls J.M., Yuen K.Y. and Peiris J.S.M., The aetiology, origins, and diagnosis of severe acute respiratory syndrome., Lancet Infectious Diseases. 2004, 4(11): 663-71.

Qu D., Zheng B., Yao X., Guan Y., Yuan Z.H., Zhong N.S., Lu L., Xie J. and Wen Y., Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) in mice induced local and serum antibodies., Vaccine. 2005, 23: 924-931.

Webster R.G., Lipatov A.S., Govorkova E.A., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webby R., Evolution of H5N1 influenza viruses in Asia., Nova Acta Leopoldina. 2005, 344: 75-80.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Zheng B., He M.L., Wong K.L., Lum C.T., Poon L.L.M., Peng Y., Guan Y., Lin M.C. and Kung H., Potent inhibition of SARS-associated coronavirus (SCoV) infection and replication by type I interferons (IFN-alpha/beta)but not by type II interferon (IFN-gamma), Journal of Interferon and Cytokine Research. 2004, 24(7): 388-90.

Zheng B., Guan Y. and Yuen K.Y., SARS – from animals to man: New Viral Threats to Human Health Worldwide, 12° CONGRESSO NAZIONALEdella SOCIETÀ di MICROBIOLOGIA MEDICA ODONTOIATRICA e CLINICA. 20-22 July, 2004. Genova, Italy.. 2004.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : He ML

Project Title:	Anti-HBV gene therapy using recombinant siRNA-rAAV virus
Investigator(s):	He ML
Department:	Institute of Molecular Biology
Source(s) of Funding:	Seed Funding Programme for Applied Research
Start Date:	06/2003
Completion Date:	09/2004

Abstract:

To construct rAAV plasmid; to package and purify rAAV virus; to test the efficacy of anti-HBV replication in vitro; to test the synergic effects of siRNAs and lamuvidine in vitro.

Project Title:	Transcriptional and functional regulation of TBX3 in retina formation
Investigator(s):	He ML, Kung H, Peng Y, Rao Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003
Completion Date:	01/2005

Abstract:

To characterize TBX3 promoter and identify elements that are responsible for the inhibition of retina formation; to characterize whether TBX3 has common and distinct function with TBX2 in retina formation; to elucidate the roles and relationship with other molecules (BMP4, jun/fos, E2F1) in the BMP4-pRB-p53 signaling pathway.

Project Title:	Transcriptional and functional regulation of TBX3 in retina formation
Investigator(s):	He ML, Kung H, Peng Y, Rao Y.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003
Completion Date:	01/2005

Abstract:

Project Title:	Development of lentiviral vectors for gene therapy
Investigator(s):	He ML
Department:	Institute of Molecular Biology
Source(s) of Funding:	Seed Funding Programme for Applied Research
Start Date:	06/2004
Completion Date:	06/2005

Abstract:

To construct pLenti expression vector; to package and purify Lentiviruses; to test transduction efficiency; to test in vitro anti-HBV efficacy.

Project Title:	The mechanisms of a novel HCC-associated oncogene (HCAO) in hepatocellular carcinogenesis
Investigator(s):	He ML, Kung H, Li T.P.
Department:	Institute of Molecular Biology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005
Completion Date:	01/2005

Abstract:

To investigate the effect of forced over-expression or suppression of HCAO in cell proliferation, cell division, cell cycle control, apoptosis, and tumorigenesis; to elucidate signaling pathways mediated by HCAO; to identify the interacting proteins of HCAO and their functions in hepatocarcinogenesis.

Project Title:	The mechanisms of a novel HCC-associated oncogene (HCAO) in hepatocellular carcinogenesis
Investigator(s):	He ML
Department:	Institute of Molecular Biology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005
Completion Date:	01/2005

Abstract:

List of Research Outputs

He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : He Z

List of Research Outputs

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Researcher : Ho PL

Project Title:	A phenotypic and genotypic analysis of vancomycin tolerance in Streptococcus pnemoniae
Investigator(s):	Ho PL, Yuen KY, Que TL
Department:	Microbiology
Source(s) of Funding:	Low Budget High Impact Programme
Start Date:	11/2000

Abstract:

To identify the frequency of occurrence of vancomycin tolerance in Streptococcus pneumoniae; to identify novel mutations in the histdine kinase (vncS) of tolerant pneumococci.

Project Title:	Molecular epidemiology of fluoroquinolone-resistant streptococcus pneumoniae in Hong kong and Canada
Investigator(s):	Ho PL, Low D.E.
Department:	Microbiology
Source(s) of Funding:	Low Budget High Impact Programme
Start Date:	11/2001

Abstract:

To define the molecular epidemiology of fluoroquinolone-resistant strains of S. pneumoniae isolated in Hong Kong and Canada.

Project Title:	Clinical outcome and impact on health care utilization of invasive pneumococcal disease in the era of antimicrobial resistance
Investigator(s):	Ho PL, Cheng YH, Chiu SSS, Tsang KWT, Yung R.W.H., Que T.L., Tsang D.N.C., Ng T.K.
Department:	Microbiology
Source(s) of Funding:	Health Care and Promotion Fund - Research Grant Projects
Start Date:	08/2002

Abstract:

To determine the impact of antibiotic resistance on patient outcome in invasive pneumococcal disease and to what extent additional health care resources are utilized as a result of the antibiotic resistance in a cohort of patients between 1997 to 2000; to document the serotypes of S. pneumoniae that casue invasive infection in the same cohort of patients.

Project Title:	Relation between antibiotic resistance in Escherichia coli of human and poultry origins
Investigator(s):	Ho PL, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To examine the relationship of antibiotic-resistant Escherichia coli isolated from poultry, hospital patients, and normal people.

Project Title:	Risk factors for SARS in health care workers following incubation of SARS patients
Investigator(s):	Ho PL, Hung CT, Yap F., Law K.I., Ho E.
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To identify factors associated with SARS transmission in HCW who participated in intubation of SARS patients; to define the transmission probability of an intubation procedure of SARS patient and to describe the intubation procedural practice of clinicians in the first two months of the outbreak.

Project Title:	Fluoroquinolone susceptibility clinical breakpoints and fluoroquinolone-resistant determining mutations among clinical strains of streptococcus pneumoniae
Investigator(s):	Ho PL, Wong SSY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To determine the frequency of occurrence of first step fluoroquinolone-resistant determining mutations among isolates categorized as susceptible by the currently accepted clinical breakpoints.

List of Research Outputs

Ho J.C.M., Wu A.Y.Y., Lam B., Ooi C.G.C., Khong P.L., Ho P.L., Chan M.M.W., Zhong N.S., Ko K.W., Lam W.K. and Tsang K.W.T., Pentaglobin in steroid-resistant severe acute respiratory syndrome, International Journal of Tuberculosis and Lung Disease. 2004, 8(10): 1173-1179.

Ho P.L., Biofunctional magnetic nanoparticles for pathogen detection , US non-provisional application no. 11/159,882 filed 23 June, 2005.. 2005.

Ho P.L., CA-MRSA: confusion with HA-MRSA, Scientific meeting. Hong Kong Medical Association / Hong Kong Society of Infectious Diseases. Hong Kong.. 2004.

Ho P.L., Common errors in infection control, International Chemotherapy Congress 2005. International Chemotherapy Congress. Manila, The Phillipines.. 2005.

Ho P.L., Community-acquired MRSA: confusion with healthcare-associated MRSA, Seminar in infectious diseases-Private Public interface in infectious diseases. Hong Kong Medical Association/HK Society of Infectious Diseases. Hong Kong.. 2004.

Ho P.L., Tse C.W., Mak C.K., Chow K.H. and Ng T.K., Community-acquired methicillin-resistant Staphylococcus aureus arrives in Hong Kong., Journal of Antimicrob Chemotherapy. 2004, 54(4): 845-6.

Ho P.L., Chow K.H., Mak G.C., Tsang K.W.T., Lau Y.L., Ho A.Y., Lai E.L. and Chiu S.S.S., Decreased Levofloxacin Susceptibility in Haemophilus influenzae in Children, Hong Kong, Emerging Infectious Diseases. 2004, 10(11): 1960-1962.

Ho P.L., Detection and characterization of extended-spectrum beta-lactamases among blood isolates of Enterobacters in Hong Kong. M.Med.Sci. thesis submitted by Mr. Shek Hoi Leong. Aug. 2004., 2004.

Ho P.L., Detection and characterization of extended-spectrum beta-lactamases among blood isolates of Proteus mirabilis in Hong Kong. M.Med.Sci. thesis submitted by Mr. Ho Yat Man Alex. Aug. 2004., 2004.

Ho P.L., Shek R.H., Chow K.H., Duan R., Mak C.K., Lai E.L.Y., Yam W.C., Tsang K.W.T. and Lai W.M., Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002., Jouranl of Antimicrobial Chemotherapy. 2005, 55(3): 326-32.

Ho P.L., Que T.L., Chiu S.S.S., Yung R.W.H., Ng T.K., Tsang D.N.C., Seto W.H. and Lau Y.L., Fluoroquinolone and Other Antimicrobial Resistance in Invasive Pneumococci, Hong Kong, 1995-2001, Emerging Infectious Diseases. 2004, 10(7): 1250-1257.

Ho P.L., Infection control in SARS, avian influenza and tuberculosis, Hospital authority training in infection control. Hospital Authority.. 2005.

Ho P.L., Mycobacterium tuberculosis and resistance to first line therapy, 9th Congress of the Asian Pacific Society of Respirology. Hong Kong Thoracic Society / American College of Chest Physicians (Hong Kong and Macau Chapter)/Hong Kong Lung Foundation. Hong Kong.. 2004.

Ho P.L., New anti-infective for respiratory infections, Annual Scientific Meeting 2005. Hong Kong Thoracic Society / American College of Chest Physicians (Hong Kong and Macau Chapter). Hong Kong.. 2005.

Ho P.L., Ho A.Y., Chow K.H., Wong R.C., Duan R.S., Ho W.L., Mak G.C., Tsang K.W., Yam W.C. and Yuen K.Y., Occurrence and molecular analysis of extended-spectrum {beta}-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002., The Journal of antimicrobial chemotherapy. 2005, 55(6): 840-5.

Ho P.L., Resistance in Staphylococcus aureus in the Far East, The 10th International Symposium on Staphylococci & Staphylococcal Infections. International Symposium on Staphylococci & Staphylococcal Infections. Alaska, United States.. 2004.

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distribution and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong children., Vaccine. 2004, 22(25-26): 3334-9.

Kwok P.W., Leung Y.H., Sun J.Z., Yan C.P.K., Ooi C.G.C., Ho P.L., Ho J.C.M., Tipoe G.L., Lam W.K. and Tsang K.W.T., The Effects of Gelomyrtol Forte on Human Ciliary Beat Frequency in vitro, Abstract Book of the 10th Medical Research Conference, University Department of Medicine, The University of Hong Kong, 19-20 February. 2005, page 65.

Kwok R., Leung Y.H., Sun J.Z., Yan C.P.K., Ooi C.G.C., Ho P.L., Ho J.C.M., Tipoe G.L., Lam W.K. and Tsang K.W.T., The effects of gelomyrtol forte on human ciliary beat frequency in vitro, Respirology. 2004, 9 (Suppl): A162 (290).

Lau S.K.P., Ho P.L., Li M.W., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and characterization of a chromosomal class C beta-lactamase and its regulatory gene in Laribacter hongkongensis., Antimicrobial agents and chemotherapy. 2005, 49(5): 1957-64.

Mak J.C.W., Ho S.P., Leung Y.H., Ho P.L., Ooi C.G.C., Yan C.P.K., Ko K.W., Ip M.S.M., Lam W.K. and Tsang K.W.T., Elevated levels of transforming growth factor- beta 1 in serum of patients with stable bronchiectasis, 9th Congress of Asian Pacific Society of Respirology, Hong Kong, December 2004. Respirology. 2004, 9 (Supp): A134.

Tsang K.W.T., Tipoe G.L., Mak J.C.W., Sun J.Z., Wong M.P., Leung R.C.M., Tan K.C.B., MedStat C.K.M., Ho J.C.M., Ho P.L., Rutman A. and Lam W.K., Ciliary central microtubular orientation is of no clinical significance in bronchiectasis, Respiratory Medicine. 2005, 99: 290-297.

Tsang K.W.T., Ooi C.G.C. and Ho P.L., Diagnosis and pharmacotherapy of severe acute respiratory syndrome: what have we learnt?, European Respiratory Journal. 2004, 24(6): 1025-1032.

Tsang K.W.T., Tan K.C.B., Ho P.L., Ooi C.G.C., Khong P.L., Leung Y.H., Mak J.C.W., Tipoe G.L., Ko K.W. and Lam W.K., Exhaled nitric oxide in bronchiectasis: the effects of inhaled corticosteroid therapy, International Journal of Tuberculosis and Lung Disease. 2004, 8(11): 1301-1307.

Tsang K.W.T., Tan K.C.B., Ho P.L., Ooi C.G.C., Ho J.C., Mak J.C.W., Tipoe G.L., Ko C., Yan C. and Lam W.K., Inhaled fluticasone in bronchiectasis: a 12 month study, Thorax. 2005, 60: 239-243.

Wang T.K.F., Ho P.L. and Wong S.S.Y., Recurrent Salmonella bacteraemia caused by different Salmonella species in a systemic lupus erythematosus patient. , New Microbiologica. 2005, 28: 151-156.

Yam W.C., Tam C.M., Leung C.C., Chan K.H., Leung T.Y., Wong K.C., Yew W.W., Seto W.H., Yuen K.Y. and Ho P.L., Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA sequencing., J Clin Microbiol . USA, ASM, 2004, 42: 4438-4443.

Yap F.H., Gomersall C.D., Fung K.S., Ho P.L., Ho O.M., Lam P.K., Lam D.T., Lyon D.J. and Joynt G.M., Increase in methicillin-resistant Staphylococcus aureus acquisition rate and change in pathogen pattern associated with an outbreak of severe acute respiratory syndrome., Clinical Infectious Diseases. 2004, 39(4): 511-6.

Yap F.H., Ho P.L. and Joynt G.M., Reply to bassetti et Al., Clinical Infectious Diseases. 2005, 40(4): 634-5.

Yap F.H.Y., Ho P.L., Lam K.F., Chan K.S., Cheng Y.H. and Peiris J.S.M., Excess hospital admissions for pneumonia, chronic obstructive pulmonary diseases, and heart failure during influenza seasons in Hong Kong, Journal of Medical Virology. Wiley-Liss, Inc., 2004, 73: 617-623.

Researcher : Huang Y

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Lau S.K.P., Wong B.H., Chan K.H., Chu C.M., Tsoi H.W., Huang Y., Peiris J.S.M. and Yuen K.Y., Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus, Clinical and diagnostic laboratory immunology. 2004, 11(4): 665-8.

Researcher : Hung IFN

List of Research Outputs

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Researcher : Im SWK

Project Title:	Investigation of community exposure to a novel coronavirus associated with SARS and herd immunity developed among healthy blood donors
Investigator(s):	Im SWK, Yam WC, Lee C.K.
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To determine the exposure of the Hong Kong community to the novel coronavirus and the extent of herd immunity developed; to optimize a cost-effective protocol suitable for high throughput blood testing screening.

Project Title:	Dendritic cell based immune intervention of nasopharyngeal carcinoma
Investigator(s):	Im SWK, Zheng B, Chua DTT
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To conduct an effective, individual EBV antigen expression system which will allow us to obtain large amount of such antigens; to detect if the DCs from NPC patients are selectively functionally defective to individual EBV antigens, and if so, whether cytokine-activate DCs may overcome the deficiency; to ascertain whether the cytokine-activated DCs of NPC patients plus the antigens can ex vivo activate specific CTL precursors or stimulate naive T cells to become specific CTLs, including optimization of the conditions for expansion of these specific (CTL cell lines in vitro; to evaluate whether the activated DCs together with the EBV antigens may indeed stimulate the target specific CTLs in NPC patients after removal of the cancer by traditional therapy.

Researcher : Kam YW

List of Research Outputs

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E. .W., Vogel L., Chu S.L., Chan C.M., Tse K.S., Subarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Immunization with trimeric S glycoprotein protects from SARS-CoV challenge despite the presence of in vitro facilitating antibodies, Xth International Nidovirus Symposium,Toward Control of SARS and Other Nidovirus Diseases. Colorado Springs, 2005.

Nal-Rogier B.T.M., Chan C.M., Kien F.S., Siu Y.L., Tse K.S., Chu S.L., Kam Y.W., Staropoli I., Crescenzo-Chaigne B., Escriou N., van der Werf S., Yuen K.Y. and Altmeyer R.M., Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E, Journal Of General Virology. Great Britain, 2005, 86: 1423-1434.

Researcher : Kam YW

List of Research Outputs

Researcher : Kao RYT

Project Title:	The application of high-throughput screening (HTS) technologies to identify inhibitors of SARS-associated coronavirus main proteinase
Investigator(s):	Kao RYT, Yuen KY
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To explore technologies for identifying inhibitors of SARS-associated coronavirus main proteinase.

Project Title:	Application of chemical genetic approaches to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei
Investigator(s):	Kao RYT, Yuen KY, Sun H, Madar J.C.S.
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

In conjunction with conventional genetic approaches (such as gene cloning and gene inactivation, etc.), we set out to employ chemical genetic approach - the use of high-throughput screening (HTS) technologies to identify biologically active small molecules that will interfere with particular cellular processes/biological pathways/gene products - to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei. Forward chemical genetics will be used to identify small molecule compounds that will interfere with host-pathogen interactions and dimorphism in P. marneffei. Reverse chemical genetics will be used to identify small molecules that will have specific inhibitory effects on MetAP1 or MetAP2 (essential enzymes involved in removal of N-terminal methionine) of P. marneffei. Resulting specific inhibitors of MetAPs will be tested in in vitro intracellular survival and in vivo animal infection models.

Project Title:	Application of chemical genetic approaches to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei
Investigator(s):	Kao RYT, Yuen KY, Sun H, Madar J.C.S.
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

Project Title:	Cloning of SARS-associated coronavirus main proteinase (SARS-CoV M^pro and identification of SARS-CoVM^pro specific synthetic substrates
Investigator(s):	Kao RYT, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2004

Abstract:

To develop a fluorescence-based continuous enzymatic assay for the rapid evaluation of potential inhibitors of SARS-CoV M^pro; so as to lay down the foundation for researches in inhibitors of various 3C-like viral proteases.

Project Title:	Identification of small molecule inhibitors of Staphylococcus aureus methionine aminopeptidase by reverse chemical genetics
Investigator(s):	Kao RYT
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005

Abstract:

Staphylococcus aureus forms part of the normal flora of human hosts as a common inhabitant of the nasal tract and the skin of humans and animals (Peacock et al., 2001). S. aureus causes a variety of infections in humans and animals. Infections are initiated when a breach of the skin or mucosal barrier allows S. aureus access to adjoining tissues or the bloodstream. During the pre-antibiotic era, many staphylococcal invasive infections led to the death of the patient. This effect was relieved by the discovery of penicillin in the 1940s. However, by the end of 1950s, approximately 50% of hospital isolates of this bacterium were penicillin resistant. Methicillin and other semi-synthetic derivative of penicillin were then used to treat S. aureus infections. In another few years, methicillin-resistant S. aureus (MRSA) were found in hospitals, leaving vancomycin as the last line of defence against staphylococcal infections (Mann, J. and Crabbe, M.J.C., 1996). However, strains of S. aureus with intermediate resistance to vancomycin are increasingly being found (Projan, S.J., 2000). Clearly, research in the pathogenesis of S. aureus and identification of novel targets for antibacterial therapy against this pathogen is imminent.Co- and post-translational modifications have long been recognized as essential steps for the maturation of proteins for proper folding, regulation, function, targeting and eventually degradation (Bradshaw, 1989; Meinnel, Mechulam, and Blanquet, 1993; Bradshaw, Brickley, and Walker, 1998; Arfin and Bradshaw, 1988). Since protein synthesis begins at the amino-terminus, modification at this site is one of the earliest steps and most common events in protein processing (Lowther and Matthews, 2002). Translation of proteins is initiated with methionine in eukaryotes and N-formyl methionine in prokaryotes, mitochondria, and chloroplasts. The N-formyl group is removed from proteins in prokaryotes and eukaryotic organelles by a deformylase, leaving a methionine residue at the amino terminus (Meinnel, Mechulam, and Blanquet, 1993). However, the majority of mature proteins do not retain the initiator residue. In the cytosolic extract of Escherichia coli only 40% of the polypeptide chains retain the N-terminal methionine, whereas about 50% display alanine, serine, or threonine at their N-termini (Waller, 1963). Methionine aminopeptidase (MetAP) is the enzyme responsible for the catalytic cleavage of the N-terminal methionine from proteins, when the penultimate residue has a small and uncharged side-chain (Tsunasawa, Stewart, and Sherman, 1985; Huang et al., 1987; Hirel et al., 1989). MetAPs have been proven essential in Gram negative bacteria, such as E. coli and Salmonella typhimurium (Chang, McGary, and Chang, 1989; Miller et al., 1989), and in Saccharomyces cerevisiae (Li and Chang, 1995). Its physiological importance has given rise to considerable interest among researchers to design antimicrobial inhibitors that specifically target this enzyme. The vitality of this gene in Gram positive bacteria has just been experimentally illustrated by our group recently (Wong et. al. Manuscript in preparation). The demonstration of map as an essential gene in Staphylococcus aureus has validated map as an attractive drug target for antibacterial and antifungal drug development.Recent advancements in synthetic organic chemistry, molecular biology, and informatics have made possible the use of large collections of small molecules (chemical libraries) to investigate protein/chemical interactions in vitro and in vivo (Kao et al. 2002, 2004; Degterev et al., 2001; Peterson et al., 2000; Mayer et al., 1999). The term "chemical genetics” has been coined to signify the use of chemicals to systematically perturb, and thus determine, the function of proteins in the same way that mutations are used in classical genetics. In forward chemical genetics, small molecules that induce altered phenotypes in cells or organisms are identified and their cellular targets will then be determined subsequently. In reverse chemical genetics, small molecules that interact with a known, purified protein are identified in vitro and their effects on cells or organisms will then be examined. We intend to employ high-throughput screened (HTS) based reverse chemical genetics to identify small molecules that will have specific inhibitory effects on the S. aureus MetAP-I (SaMetAP-I). Resulting specific inhibitors of SaMetAP-I will be tested in cell-based assays (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, and E. coli) to determine their in vivo antimicrobial effects.

List of Research Outputs

Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75.

Kao R.Y.T., To A.P.C., Ng L.W.Y., Tsui W.H.W., Lee T.S.W., Tsoi H.W. and Yuen K.Y., Characterization of SARS-CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence-based assay., FEBS letters. 2004, 576(3): 325-30.

Tanner J.A., Watt R.M., Kao R.Y.T. and Huang J., Targeting the SARS Coronavirus Helicase - Three Approaches to Inhibitor Development, FASEB Journal. 2005, 19: 217.9.

Researcher : Lai ELY

List of Research Outputs

Ho P.L., Shek R.H., Chow K.H., Duan R., Mak C.K., Lai E.L.Y., Yam W.C., Tsang K.W.T. and Lai W.M., Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002., Jouranl of Antimicrobial Chemotherapy. 2005, 55(3): 326-32.

Researcher : Lai WM

List of Research Outputs

Ho P.L., Shek R.H., Chow K.H., Duan R., Mak C.K., Lai E.L.Y., Yam W.C., Tsang K.W.T. and Lai W.M., Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002., Jouranl of Antimicrobial Chemotherapy. 2005, 55(3): 326-32.

Researcher : Lam HY

List of Research Outputs

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Researcher : Lam SY

List of Research Outputs

Chiang A.K.S., Lam S.Y. and Chan K.H., Kinetics of EBV-specific Antibody in Symptomatic and Asymptomatic Primary Epstein-Barr Virus Infection in Chinese Children, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 46.

Guan M., Chan K.H., Peiris J.S.M., Kwan S.W., Lam S.Y., Pang C.M., Chu K.W., Chan K.M., Chen H.Y., Phuah E.B. and Wong C.J., Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome., Clinical and Diagnostic Laboratory Immunology. 2004, 11(4): 699-703.

Researcher : Lau SKP

Project Title:	Molecular characterization of anaerobic Gram-positive non-sporulating rods and its clinical significance
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2002
Completion Date:	08/2004

Abstract:

The study proposes to use 16S ribosomal RNA gene sequencing as a way of genotypic identification for all clinical isolates of this group of bacteria failed to be identified by conventional methods. Using this approach, the research team aims at guiding clinicians to more appropriate treatment regimen and a better characterization of the clinical spectrum of diseases.

Project Title:	Molecular characterization of Streptococcus iniae isolated from patients outside North America
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To define the 2 isolates by 16S ribosomal RNA gene sequencing and perform PEGE on these 2 isolates, together with a type strain and the disease-associated strain from Canada.

Project Title:	Detection of nucleocapsid protein in patients with SARS related coronavirus infections
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To develop an enzyme-linked immunosorbant assay (ELISA)-based antigen test for rapid diagnosis of Severe Acute Respiratory Syndrome (SARS).

Project Title:	Molecular characterization and pathogenesis of a rare bacterium causing psoas abscess
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To characterize the pathogenesis of a rare bacterium associated wth psoas abscess by sequencing its 16S rRNA gene, M protein gene (emm) and streptolysin S structural gene (sagA); to characterize the bacterium's clonality by mulit-locus sequence typing, thus allowing an estimation on the potential of similar diseases in other parts of the world.

Project Title:	Usefulness of microseq 500 16S rDNA identification system for identification of anaerobic gram-positive bacilli isolated from blood cultures
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To evaluate the usefulness of the MicroSeq 500 16S rDNA bacterial identification system in the identification of anaerobic gram-positive bacilli isolated from blood cultures; to compare the results from the MicroSeq system with those from commercial biochemical identification kit.

List of Research Outputs

Chow C.K., Wong S.S.Y., Ho A.C.W. and Lau S.K.P., Unilateral epistaxis after swimming in a stream., Hong Kong Medical Journal. 2005, 11: 110-112.

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Lau S.K.P., Woo P.C.Y., Fung A.M., Chan K.M., Woo G.K.S. and Yuen K.Y., Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing., Journal of medical microbiology. 2004, 53(Pt 12): 1247-53.

Lau S.K.P., Ho P.L., Li M.W., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and characterization of a chromosomal class C beta-lactamase and its regulatory gene in Laribacter hongkongensis., Antimicrobial agents and chemotherapy. 2005, 49(5): 1957-64.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Lau S.K.P., Woo P.C.Y., To P.C.A., Lau T.Y. and Yuen K.Y., Lack of evidence that DNA in antibiotic preparations is a source of antibiotic resistance genes in bacteria from animal or human sources., Antimicrob Agents Chemother. 2004, 48(8): 3141-6.

Lau S.K.P., Woo P.C.Y., Chan C.Y., Wu W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi., Journal of clinical microbiology. 2005, 43(3): 1470-2.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Eco-epidemiology of Laribacter Hongkongensis, a novel bacterium associated with gastroenteritis, 9th Research Postgraduate Symposium., 2004.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H., Ng L.T., Hui W.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis., Journal of clinical microbiology. 2005, 43(2): 919-22.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess., Systematic and applied microbiology. 2005, 28(4): 316-22.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia., Microbiology and immunology. 2005, 49(1): 31-9.

Woo P.C.Y., Lau S.K.P., Woo G.K.S., Fung A.M., Yiu V.P. and Yuen K.Y., Bacteremia due to Clostridium hathewayi in a patient with acute appendicitis., Journal of clinical microbiology. 2004, 42(12): 5947-9.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Wong M.K.M. and Yuen K.Y., Bacteremia in a patient with colonic carcinoma caused by a novel Sedimentibacter species: Sedimentibacter hongkongensis sp. nov., Diagnostic microbiology and infectious disease. 2004, 50(2): 81-7.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Biliary tract disease as a risk factor for Plesiomonas shigelloides bacteraemia: a nine-year experience in a Hong Kong hospital and review of the literature., The new microbiologica. 2005, 28(1): 45-55.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M., Tang S.F. and Yuen K.Y., Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing., Journal of clinical pathology. 2005, 58(3): 301-7.

Woo P.C.Y., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., Continuous ambulatory peritoneal dialysis-related peritonitis associated with Lancefield group G beta-hemolytic streptococcus: report of two cases requiring Tenckhoff catheter removal., Journal of clinical microbiology. 2004, 42(9): 4399-402.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Chan K.H., Hui W.T., Kwan G.S., Peiris J.S.M., Couch R.B. and Yuen K.Y., False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide., Journal of clinical microbiology. 2004, 42(12): 5885-8.

Woo P.C.Y., Lau S.K.P., Wong B.H., Chan K.H., Chu C.M., Tsoi H.W., Huang Y., Peiris J.S.M. and Yuen K.Y., Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus, Clinical and diagnostic laboratory immunology. 2004, 11(4): 665-8.

Woo P.C.Y., Lau S.K.P., Lau C.C., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient., Bone marrow transplantation. 2005, 35(8): 831-3.

Woo P.C.Y., To P.C.A., Lau S.K.P., Fung A.M. and Yuen K.Y., Phenotypic and molecular characterization of erythromycin resistance in four isolates of Streptococcus-like gram-positive cocci causing bacteremia., Journal of clinical microbiology. 2004, 2004: 3303-5.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Tse H., Wong B.H.L. and Yuen K.Y., Streptococcus sinensis may react with Lancefield group F antiserum., Journal of medical microbiology. 2004, 53(Pt 11): 1083-8.

Researcher : Lee HC

List of Research Outputs

Poon L.L.M., Leung C.S.W., Chan K.H., Lee H.C., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of human influenza A viruses by loop-mediated isothermal amplification., Journal of Clinical Microbiology. 2005, 43(1): 427-30.

Researcher : Lee SS

List of Research Outputs

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zhao X.Y., Lee S.S., Wong K.H., Chan K.C., Ma S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Effects of single nucleotide polymorphisms in the RANTES promoter region in healthy and HIV-infected indigenous Chinese., European journal of immunogenetics. 2004, 31(4): 179-83.

Zheng B., Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ma S. and Ng M.H., Effects of single nucleotide polymorphism in the RANTES promoter region on HIV infection and disease progression in indigenous Chinese, XV international AIDS conference. 11-16 July, 2004. Bangkok, Thailand.. 2004.

Researcher : Lee TSW

List of Research Outputs

Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75.

Kao R.Y.T., To A.P.C., Ng L.W.Y., Tsui W.H.W., Lee T.S.W., Tsoi H.W. and Yuen K.Y., Characterization of SARS-CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence-based assay., FEBS letters. 2004, 576(3): 325-30.

Researcher : Leung CSW

List of Research Outputs

Poon L.L.M., Leung C.S.W., Chan K.H., Lee H.C., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of human influenza A viruses by loop-mediated isothermal amplification., Journal of Clinical Microbiology. 2005, 43(1): 427-30.

Researcher : Leung CYH

List of Research Outputs

Choi Y.K., Ozaki H., Webby R.J., Webster R.G., Peiris J.S.M., Poon L.L.M., Butt C., Leung C.Y.H. and Guan Y., Continuing evolution of H9N2 influenza viruses in Southeastern China., Journal of virology. 2004, 78(16): 8609-14.

Ellis T.M., Leung C.Y.H., Chow M.K., Bissett L.A., Wong W., Guan Y. and Peiris J.S.M., Vaccination of chickens against H5N1 avian influenza in the face of an outbreak interrupts virus transmission., Avian pathology. 2004, 2004: 405-12.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Researcher : Leung TY

List of Research Outputs

Yam W.C., Tam C.M., Leung C.C., Chan K.H., Leung T.Y., Wong K.C., Yew W.W., Seto W.H., Yuen K.Y. and Ho P.L., Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA sequencing., J Clin Microbiol . USA, ASM, 2004, 42: 4438-4443.

Researcher : Li WS

List of Research Outputs

Li W.S., Cloning and characterization of chromosomal class C B-Lactamase and its regulatory gene in Laribacter hongkongensis (MPhil thesis), supervisor: Yuen K.Y., Woo P.C.Y., 2005.

Li W.S., Zhang C., Sui J., Kuhn H., Moore M.J., Luo S., Wong S.K., Huang I.C., Xu K., Vasilieva N., Murakami A., He Y., Marasco W.A., Guan Y., Choe H. and Farzan M., Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. , The EMBO journal. 2005, 24(8): 1634-1643.

Researcher : Lin Y

List of Research Outputs

Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.

Researcher : Luk W

List of Research Outputs

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells, Blood. 2005, 106(7): 2366-2374.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine upregulation in SARS coronavirus infected human monocyte derived dendritic cells, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 85.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Human Monocyte Derived Dendritic Cells Infected by SARS Associated Coronavirus, 12th International Congress of Immunology and 4th Annual Conference of FOCIS, Montreal, Canada, 18-23 July 2004.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Peiris J.S.M. and Lau Y.L., SARS Coronavirus Induced Chemokines Expression in Human Monocyte Derived Dendritic Cells, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 48.

Researcher : Luk WK

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Researcher : Luo S

List of Research Outputs

Li W.S., Zhang C., Sui J., Kuhn H., Moore M.J., Luo S., Wong S.K., Huang I.C., Xu K., Vasilieva N., Murakami A., He Y., Marasco W.A., Guan Y., Choe H. and Farzan M., Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. , The EMBO journal. 2005, 24(8): 1634-1643.

Researcher : Ma S

List of Research Outputs

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X.Y., Lee S.S., Wong K.H., Chan K.C., Ma S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Effects of single nucleotide polymorphisms in the RANTES promoter region in healthy and HIV-infected indigenous Chinese., European journal of immunogenetics. 2004, 31(4): 179-83.

Zheng B., Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ma S. and Ng M.H., Effects of single nucleotide polymorphism in the RANTES promoter region on HIV infection and disease progression in indigenous Chinese, XV international AIDS conference. 11-16 July, 2004. Bangkok, Thailand.. 2004.

Researcher : Ma SSL

List of Research Outputs

Ma S.S.L., Genetic manipulation systems in Laribacter hongkongensis, a novel bacterium associated with gastroenteritis (MPhil thesis), supervisor: Woo P.C.Y., Lau S.K.P., 2005.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Eco-epidemiology of Laribacter Hongkongensis, a novel bacterium associated with gastroenteritis, 9th Research Postgraduate Symposium., 2004.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H., Ng L.T., Hui W.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis., Journal of clinical microbiology. 2005, 43(2): 919-22.

Researcher : Mak CK

List of Research Outputs

Ho P.L., Tse C.W., Mak C.K., Chow K.H. and Ng T.K., Community-acquired methicillin-resistant Staphylococcus aureus arrives in Hong Kong., Journal of Antimicrob Chemotherapy. 2004, 54(4): 845-6.

Ho P.L., Shek R.H., Chow K.H., Duan R., Mak C.K., Lai E.L.Y., Yam W.C., Tsang K.W.T. and Lai W.M., Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002., Jouranl of Antimicrobial Chemotherapy. 2005, 55(3): 326-32.

Researcher : Ng F

List of Research Outputs

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Researcher : Ng IHY

List of Research Outputs

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells, Blood. 2005, 106(7): 2366-2374.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine upregulation in SARS coronavirus infected human monocyte derived dendritic cells, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 85.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Human Monocyte Derived Dendritic Cells Infected by SARS Associated Coronavirus, 12th International Congress of Immunology and 4th Annual Conference of FOCIS, Montreal, Canada, 18-23 July 2004.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Peiris J.S.M. and Lau Y.L., SARS Coronavirus Induced Chemokines Expression in Human Monocyte Derived Dendritic Cells, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 48.

Researcher : Ng LWY

List of Research Outputs

Kao R.Y.T., To A.P.C., Ng L.W.Y., Tsui W.H.W., Lee T.S.W., Tsoi H.W. and Yuen K.Y., Characterization of SARS-CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence-based assay., FEBS letters. 2004, 576(3): 325-30.

Researcher : Ng MH

List of Research Outputs

Cao K., Zheng Y., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H. and Zheng B., Peripheral NK Cells Play a Complementary Role for Anti-tumor Immunity in Nasopharyngeal Carcinoma Patients., Keystone Symposia – Basic Aspects of Tumor Immunology II (C3). Keystone, Colorado, USA.. 2005.

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Ma L., Ng M.H. and Huang F., Interactions of dendritic cells and dying cells in Systemic Lupus Erythematosus, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zhao X.Y., Lee S.S., Wong K.H., Chan K.C., Ma S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Effects of single nucleotide polymorphisms in the RANTES promoter region in healthy and HIV-infected indigenous Chinese., European journal of immunogenetics. 2004, 31(4): 179-83.

Zheng B., Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ma S. and Ng M.H., Effects of single nucleotide polymorphism in the RANTES promoter region on HIV infection and disease progression in indigenous Chinese, XV international AIDS conference. 11-16 July, 2004. Bangkok, Thailand.. 2004.

Researcher : Ng SF

List of Research Outputs

Cheung K.W., Guan Y., Ng S.F., Chen H., Wong C.H.K., Peiris J.S.M. and Poon L.L.M., Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron., Journal of General Virology. 2005, 86(Pt 5): 1447-54.

Researcher : Ng SP

Project Title:	Monoclonal antibodies against lipopolysaccharide antigens of Salmonella and Shigella spp.
Investigator(s):	Ng SP
Department:	Microbiology
Source(s) of Funding:	Research Initiation Programme
Start Date:	02/2001

Abstract:

To characteerise a panel of monoclonal antibodies raised against the outer core region of Salamonella lipopolysaccharides (LPS).

Project Title:	Collection of background information on the alternative faecal indicators in the environmental waters of Hong Kong and their correlation with Escherichia coli
Investigator(s):	Ng SP, Yam WC
Department:	Microbiology
Source(s) of Funding:	Environment Protection Department - General Award
Start Date:	06/2001

Abstract:

To detect and enumerate alternative faecal microbial indicator organisms in the environmental waters of Hong Kong and to correlate the results with Escherichia coli in order to assess their value in water quality monitoring.

List of Research Outputs

Cao K., Zheng Y., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H. and Zheng B., Peripheral NK Cells Play a Complementary Role for Anti-tumor Immunity in Nasopharyngeal Carcinoma Patients., Keystone Symposia – Basic Aspects of Tumor Immunology II (C3). Keystone, Colorado, USA.. 2005.

Ching W.H., Leung M.K.H., Leung Y.C., Yam W.C., Ng S.P. and Vrijmoed L.L.P., Photocatalytic degradation of gaseous formaldehyde by Sol-Gel TiO₂ thin film coated on glass, Proceedings of 2004 Asia-Pacific Conference of International Solar Energy Society (ISES). Gwangju, Korea, 2004, 561-567.

Researcher : Pang CM

List of Research Outputs

Guan M., Chan K.H., Peiris J.S.M., Kwan S.W., Lam S.Y., Pang C.M., Chu K.W., Chan K.M., Chen H.Y., Phuah E.B. and Wong C.J., Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome., Clinical and Diagnostic Laboratory Immunology. 2004, 11(4): 699-703.

Researcher : Peiris JSM

Project Title:	Human herpesvirus 6 (HHV-6) and HHV-7 infections: reactivation, latency and viral interactions
Investigator(s):	Peiris JSM, Chiu SSS
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2001

Abstract:

To investigate patients with primary HHV-6 or HHV-7 inection, lateny and reactivation to: a) define the transcription patterns of viral genes in these different states, b) study the effect of related herpesviral infections on the reactivation of HHV-6, HHV-7 and CMV from latency in-vivo. To establish in-vitro models of HHV-6 and HHV-7 latency and reactivation to a) define the transcription patterns of viral genes in productive replication, latency and reactivation, b) study the effect of viral interactions on reactivation from latency, c) study the effect of cross-reacting antibody on the establishment of infection and latency in monocytes and peripheral blood leukocytes.

Project Title:	The Zoonotic potential of influenze viruses in Southeastern China
Investigator(s):	Peiris JSM, Guan Y, Shortridge KF, Webster R.G.
Department:	Microbiology
Source(s) of Funding:	The Wellcome Trust - General Award
Start Date:	05/2002
Completion Date:	04/2005

Abstract:

To establish the epidemiological dynamics and a spatially structured ecological / epidemiological model of influenza in poultry and pigs in Hong Kong and mainland China that provide conditions for interspecies transmission of influenza viruses; to determine if certain genotypes of influenza viruses have pandemic potential.

Project Title:	The application of DNA arrays to characterise the avian influenza gene pool in southern China
Investigator(s):	Peiris JSM, Guan Y, Shortridge KF, Yang MMS, Li K.S.
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2002

Abstract:

This project is a multi-institutional collaborative study for developing a high throughput DNA array to allow genotyping of influenza virus isolates of avian origin from Hong Kong and applying it to characterize the influenza virus gene pool in sourthern China. Through its application, it is expected to:- 1) identify the precursor virus that donated its internal genes to generate the mutiple reassortment of H5N1 isolated in Hong Kong markets in 2001; 2) define the frequency of reassortment between viruses of different virus lineages that co-circulate in poultry in Hong Kong and correlate this to host-specificity; and 3) investigate whether avian internal gene segments are transiently introduced into the human influenza gene pool.

Project Title:	Influenza pandemic preparedness in Asia (Research collaboration with national Institute of Allergy and Infectious Diseases (NIAID), St. Jude Children's Research Hospital, Inc. (SJCRH) and The University of Hong Kong)
Investigator(s):	Peiris JSM, Guan Y
Department:	Microbiology
Source(s) of Funding:	National Institutes of Health, US Department of Health and Human Services - General Award
Start Date:	04/2003

Abstract:

To study influenza pandemic preparedness in Asia.

Project Title:	Virus-cell interaction and the pathogenesis of SARS
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To explore what is the biological basis for the selective attraction of macrophages to the lung; to study what immunopathological consequences follow.

Project Title:	Genetic variation of the SARS coronavirus: molecular epidemiology and antigenic variation
Investigator(s):	Peiris JSM, Hedley AJ, Yuen KY, Guan Y, Leung FCC, Leung GM, Poon LLM, Lam TH, Anderson R.M., Chan P.K.S., Sung J.J.Y., Lim W.W.L.
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (Special Grants for Severe Acute Respiratory Syndrome Research)
Start Date:	08/2003
Completion Date:	07/2004

Abstract:

To establish a comprehensive integrated clinical specimen and viral genetic sequence database on SARS-CoV in Hong Kong; to define the molecular epidemiology of the virus and transmission patterns within Hong Kong and the wider region; to define the genetic diversity in the S-gene region associated with antigenic change.

Project Title:	Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?
Investigator(s):	Peiris JSM, Guan Y, Lau ASY, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

To identify the viral genetic determinants responsible for the differential cytokine hyper-induction associated with H5N1/97 viruses. We have already established the role of the viral NS gene, however, a role for other viral genes has not been excluded. we hypothesize (and have preliminary evidence) that one or more other viral genes are also involved in the hyper-induction of cytokines; to determine whether the avian viral genes/gene products of H5N1/97 directly up-regulate cytokine induction or lack "immune evasion" mechanisms evolved by human influenza viruses? to determine whether the differential up-regulation of cytokines associated with H5N1/97 is also observed in human lung epithelial cells (primary bronchial epithelial cells and human lung epithelial cell lines e.g. A549 cells.

Project Title:	Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?
Investigator(s):	Peiris JSM, Guan Y, Lau ASY, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

Project Title:	The role of influenza virus gene constellation and viral morphology on cytokine induction, pathogenesis and viral virulence
Investigator(s):	Peiris JSM, Nicholls JM, Guan Y, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	06/2004

Abstract:

Refer to hard copy

Project Title:	The macrophage in the pathogenesis of SARS coronavirus infection
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

Project Title:	Virology and pathogenesis of avian influenza and SARS
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Senior Medical Research Fellowships
Start Date:	03/2005
Completion Date:	08/2005

Abstract:

To be submitted

List of Research Outputs

Chan K.H., Yau C.Y., Chu K.W., Poon L.L.M., Maldeis N.E. and Peiris J.S.M., Evaluation of Reverse Transcriptase-Strand Displacement Amplification (BD Probetec^TM) for Rapid Diagnosis of SARS Coronavirus Infection, 21st Annual Clinical Virology Symposium; Florida, USA; 8-9 May, 2005.. 2005.

Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75.

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Cheung K.W., Guan Y., Ng S.F., Chen H., Wong C.H.K., Peiris J.S.M. and Poon L.L.M., Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron., Journal of General Virology. 2005, 86(Pt 5): 1447-54.

Chiu S.S.S., Chan K.H., Chu K.W., Kwan S.W., Guan Y., Poon L.L.M. and Peiris J.S.M., Human Coronavirus NL63 Infection and Other Coronavirus Infections in Children Hospitalized with Acute Respiratory Disease in Hong Kong, China, Clinical Infectious Diseases. 2005, 40: 172-11729.

Chiu S.S.S. and Peiris J.S.M., Human Metapneumovirus Infection in the Paediatric Population, Journal of Paediatrics, Obstetrics and Gynaecology. 2004, 30(4): 141-146.

Choi Y.K., Ozaki H., Webby R.J., Webster R.G., Peiris J.S.M., Poon L.L.M., Butt C., Leung C.Y.H. and Guan Y., Continuing evolution of H9N2 influenza viruses in Southeastern China., Journal of virology. 2004, 78(16): 8609-14.

Chu C.M., Poon L.L.M., Cheng V.C.C., Chan K.S., Hung I.F., Wong M.M., Chan K.H., Leung W.S., Tang S.F., Chan V.L., Ng W.L., Sim T.C., Ng P.W., Law K.I., Tse D.M., Peiris J.S.M. and Yuen K.Y., Initial viral load and the outcomes of SARS., Canadian Medical Association journal . 2004, 171(11): 1349-52.

Chye M.L., Li H., Ramalingam S., Poon L.L.M. and Peiris J.S.M., Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS, US Patent Application No. 11/004,399 filed December 3, 2004 [Patent WO2005054473-A1 published June 16,2005] . United States Patent Office, 2005.

Ellis T.M., Leung C.Y.H., Chow M.K., Bissett L.A., Wong W., Guan Y. and Peiris J.S.M., Vaccination of chickens against H5N1 avian influenza in the face of an outbreak interrupts virus transmission., Avian pathology. 2004, 2004: 405-12.

Govorkova E.A., Rehg J.E., Krauss S., Yen H.L., Guan Y., Peiris J.S.M., Nguyen T.D., Hanh T.H., Puthavathana P., Long H.T., Buranathai C., Lim W., Webster R.G. and Hoffmann E., Lethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004., Journal of Virology. 2005, 79(4): 2191-8.

Guan M., Chan K.H., Peiris J.S.M., Kwan S.W., Lam S.Y., Pang C.M., Chu K.W., Chan K.M., Chen H.Y., Phuah E.B. and Wong C.J., Evaluation and validation of an enzyme-linked immunosorbent assay and an immunochromatographic test for serological diagnosis of severe acute respiratory syndrome., Clinical and Diagnostic Laboratory Immunology. 2004, 11(4): 699-703.

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Ip E.W.K., Chan K.H., Law H.K.W., Tso H.W., Kong K.P., Wong W.H.S., To Y.F., Yung W.W.H., Chow E.Y., Au K.L., Chan E.Y.T., Lim W., Jensenius J.C., Turner M.W., Peiris J.S.M. and Lau Y.L., Mannose-Binding Lectin in Severe Acute Respiratory Syndrome Coronavirus Infection, Journal of Infectious Diseases. 2005, 191: 1697-1704.

Kam Y.W., Kien F.S., Roberts A., Cheung C.Y., Lamirande E. .W., Vogel L., Chu S.L., Chan C.M., Tse K.S., Subarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Immunization with trimeric S glycoprotein protects from SARS-CoV challenge despite the presence of in vitro facilitating antibodies, Xth International Nidovirus Symposium,Toward Control of SARS and Other Nidovirus Diseases. Colorado Springs, 2005.

Khoo U.S., Chan Y.K., Peiris J.S.M., Yip S.P., Liu W., Ngan H.Y.S., Tam P.K.H., Chan L.C. and Cheung A.N.Y., Association of ICAM3 genetic variant with Severe Acute Respiratory Syndrome (SARS)., HUGO's 10th Human Genome Meeting, 18-21 April 2005, Kyoto, Japan. 2005.

Lam E., Poon L.L.M., Zhou Y., Chye M.L. and Peiris J.S.M., Transgenic plant-derived siRNAs: suppression of influenza virus propagation in mammalian cells, US Provisional Patent Application No. 60/673,100 filed April 20, 2005 . 2005.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells, Blood. 2005, 106(7): 2366-2374.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine upregulation in SARS coronavirus infected human monocyte derived dendritic cells, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 85.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Human Monocyte Derived Dendritic Cells Infected by SARS Associated Coronavirus, 12th International Congress of Immunology and 4th Annual Conference of FOCIS, Montreal, Canada, 18-23 July 2004.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Peiris J.S.M. and Lau Y.L., SARS Coronavirus Induced Chemokines Expression in Human Monocyte Derived Dendritic Cells, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 48.

Lee D.C.W., Li C.B., Cheung C.Y., Mok K.P., Law H.Y., Peiris J.S.M. and Lau A.S.Y., Immune Response to Virus Infections: Molecular Signaling Pathways of HIV - and H5N1-induced Cytokine Dysregulation in Disease Pathogenesis, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 13.

Leung G.M., Chung P.H., Tsang T., Lim W., Chan K.K., Chau Y.K., Donnelly C.A., Ghani A.C., Fraser C., Riley S., Ferguson N.M., Anderson R.M., Law Y.L., Mok T., Ng T., Fu A., Leung P.Y., Peiris J.S.M., Lam T.H. and Hedley A.J., SARS-CoV antibody prevalence in all Hong Kong patient contacts, Emerging Infectious Diseases. 2004, 10: 1653-1656.

Leung G.M., Chung P.H., Tsang T., Lim W., Chan K.K., Chau Y.K., Donnelly C.A., Ghani A.G., Fraser C., Riley S., Ferguson N., Anderson R.M., Law Y.L., Mok T., Ng T., Fu A., Leung P.Y., Peiris J.S.M., Lam T.H. and Hedley A.J., Seroprevalence of IgG antibody to SARS coronavirus (SARS-CoV) in a population-based sample of close contacts of all 1,755 cases in Hong Kong (Abstract), American Public Health Association 132nd Annual Meeting and Exposition, 6-10 November 2004, Washington, DC. Washington, 2004, 121.

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Lipatov A.S., Govorkova E.A., Webby R.J., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webster R.G., Influenza: emergence and control., Journal of virology. 2004, 2004: 8951-9.

Mok K.P., Lee D.C.W., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., Cytokine dysregulation and differential onset of apoptosis in H5N1- and H1N1-infected human blood macrophages. , In: Mok CKP, Lee CW, Cheung CY, Peiris M and Lau ASY. , 9th Research Postgraduate Symposium, Faculty of Medicine, The University of Hong Kong. December 2004.. 2004.

Mok K.P., Lee D.C.W., Cheung C.Y., Peiris J.S.M. and Lau A.S.Y., Differential onset of apoptosis and its mechanisms in H5N1- and H1N1-infected human blood macrophages., In: Mok CKP, Lee CW, Cheung CY, Peiris M and Lau ASY. , 34th Annual Scientific Meeting of the Australasian Society for Immunology Australia. December 2004.. 2004.

Mok K.P., Yim H.C.H., Lee D.C.W., Peiris J.S.M. and Lau A.S.Y., Molecular Mechanisms of Avian Influenza Induced Interferon Dysregulation in Human Macrophages, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 51.

Ng M.W., Law H.K.W., Yung R.W.H., Chow E.Y., Au K.L., Chan E.Y.T., Lim W., Peiris J.S.M. and Lau Y.L., Association of Monocyte Chemoattractant Protein 1 Gene Polymorphism with Severe Acute Respiratory Syndrome (SARS), Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 51.

Okada M., Takemoto Y., Okuno Y., Hashimoto S., Yoshida S., Fukunaga Y., Tanaka T., Kita Y., Kuwayama S., Muraki Y., Kanamaru N., Takai H., Okada C., Sakaguchi Y., Furukawa I., Yamada K., Matsumoto M., Kase T., Demello D.E., Peiris J.S.M., Chen P.J., Yamamoto N., Yoshinaka Y., Nomura T., Ishida I., Morikawa S., Tashiro M. and Sakatani M., The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice., Vaccine. 2005, 23 (17-18): 2269-72.

Peiris J.S.M., Co-chair of the Session: Avian Influenza. , 24th International Congress on Chemotherapy, “Confronting Health Challenges, Strengthening Strategies”. Manila, The Philippines. . 2005.

Peiris J.S.M. and Guan Y., Confronting SARS: a view from Hong Kong., Philosophical transactions of the Royal Society of London. Series B, Biological sciences. 2004, 359(1447): 1075-9.

Peiris J.S.M., Convener of the Session: SARS in 2004. , ICAAC. American Society for Microbiology. USA. . 2004.

Peiris J.S.M., Croucher Senior Medical Research Fellowship 2005-2006, 2004.

Peiris J.S.M., Hong Kong Foreign Correspondents’ Club Lucheon Talk. Title: Beyond SARS: Preparing for the next epidemic., 2004.

Peiris J.S.M., Lecture: "The Re-Emergence of SARS". , ICAAC. American Society of for Microbiology. USA. . 2004.

Peiris J.S.M., Lecture: Prion diseases and infection control., Infectious Disease Course for Surgeons 2005. Hong Kong Hospital Authority. . 2005.

Peiris J.S.M., Lecture: "Avian Influenza A (H5N1): Origins and Pathogenesis". , 24th International Congress on Chemotherapy, “Confronting Health Challenges, Strengthening Strategies”. Manila, The Philippines. . 2005.

Peiris J.S.M., Lecture: "Emergence of H5N1 virus: Its evolution and pathogenesis"., WPCCID IX Meeting. Bangkok, Thailand. . 2004.

Peiris J.S.M., Lecture: "SARS and other emerging infections"., 7th International Medical Congress. Sri Lanka. . 2004.

Peiris J.S.M., Lecture: Avian Influenza: Pandemic Potential? , Pandemic Influenza: Could History Repeat itself? University of Michigan: School of Public Health, Florida, USA. . 2005.

Peiris J.S.M., Lecture: Biology of AI Virus., National Workshop on Avian Influenza. Bogor, Indonesia. . 2005.

Peiris J.S.M., Lecture: Bloodborne infections and needlestick injuries., Infectious Disease Course for Surgeons 2005. Hong Kong Hospital Authority. . 2005.

Peiris J.S.M., Lecture: Confronting SARS and Avian Flu: Laboratory Issues., SARS-Coronaviruses and Highly Pathogenic Influenza Viruses: Safety and Occupational Health for Laboratory Workers. Albany, USA. . 2004.

Peiris J.S.M., Lecture: Gearing up for SARS: Bird Flu & Impending Human Influenza., Biotech India 2005 2nd International Exhibition and Conference on Biotechnology. India. . 2005.

Peiris J.S.M., Lecture: SARS Coronavirus and Influenza: Research Update., ASEAN PLUS THREE Expert Group Meeting on Emerging Diseases. Bangkok, Thailand. . 2005.

Peiris J.S.M., Lecture: SARS Cov., Pasteur – Asia Virology Course 2004. Institute of Pasteur, The University of Hong Kong. . 2004.

Peiris J.S.M., Lecture: SARS., Conference on Emerging and Re-emerging Diseases. Tokyo, Japan. . 2005.

Peiris J.S.M., Lecture: SARS: Aspects of pathogenesis and the animal origins of the virus., EPISARS Consortium & PRR Chinese-French Research Collaborative Network on SARS. Hong Kong. . 2005.

Peiris J.S.M., Outstanding Research Award of 2003-04, The University of Hong Kong. 2004.

Peiris J.S.M., Plenary Lecture: "A Threat to Human Health and Implications for Tourism / Travel and Lecture: Human Influenza, Seasonality in the Region and I’m travellers"., 5th Asia Pacific Travel Health Conference 95th APTHC). Bangkok, Thailand. . 2004.

Peiris J.S.M., Plenary Lecture: "SARS: Confronting a New Disease". , WPCCID IX Meeting. Bangkok, Thailand. . 2004.

Peiris J.S.M., Plenary session: Topic: Global Viral Threads “SARS update”., Eurovirology Madrid Conference. European Society for Clinical Virology (ESCV). Spain. . 2004.

Peiris J.S.M., Scientific Session: Respiratory Infections; Lecture: Confronting emerging infections: SARS and Avian Flu., Christian Medical College (CMC) III Winter Symposium on “Infection and Immunity”. Vellore, India. . 2004.

Peiris J.S.M., Session: Avian Influenza: Lecture "A threat to human health"., 11th International Conference of the Association of Institutions for Tropical Veterinary Medicine and 16th Veterinary Association Malaysia Congress. Kuala Lumpur, Malaysia. . 2004.

Peiris J.S.M., Session: Emerging Pathogens. Lecture: SARS: A new emerging disease., 5th Louis Pasteur Conference on Infectious Diseases. Paris, France. . 2004.

Peiris J.S.M., Session: Epidemiology and Virology; Lecture: Virology and Laboratory Diagnosis, Symposium on Avian Influenza – in preparation for a pandemic. Hong Kong. . 2005.

Peiris J.S.M., Session: Global Threats and Preparedness. Lecture: Severe Acute Respiratory Syndrome., 24th International Congress on Chemotherapy, “Confronting Health Challenges, Strengthening Strategies”. Manila, The Philippines. . 2005.

Peiris J.S.M., Session: Molecular Pathology; Lecture: Molecular Diagnosis of Virological infections., COC Commissioned Training Program 2004/2005. Hong Kong Hospital Authority. . 2004.

Peiris J.S.M., Session: New and Emerging Zoonoses. Lecture:The role of the human-animal interface in the emergence of new pathogenic respiratory viruses., Health Protection Agency International Zoonoses Conference. Liverpool, United Kingdom.. 2005.

Peiris J.S.M., Session: Severe Acute Respiratory Syndrome (SARS); Lecture: The etiology and pathogenesis of SARS., International Pediatric Respiratory, Allergy and Immunology Congress 2004. Hong Kong. . 2004.

Peiris J.S.M., Guan Y. and Yuen K.Y., Severe acute respiratory syndrome., Nature medicine. 2004, 10(12 Suppl): S88-97.

Peiris J.S.M., Guan Y. and Yuen K.Y., Special Research Achievement Award for the Research Team in the Department of Microbiology 2004., The University of Hong Kong. 2004.

Peiris J.S.M., The Frederick Deinhardt Lectureship. Topic: Avian Influenza: A Pandemic Threat., 21st Annual Clinical Virology Symposium. Florida, USA. . 2005.

Peiris J.S.M., The Hottest Research of 2003-04. Scientists with Multiple Hot Papers. Ranked no. 1 with 9 hot papers cited during period., Science Watch.. 2005, 16 (2): 1-8.

Peiris J.S.M., Title: Avian and Human Influenza: Experiences of Hong Kong SAR., Asean Parliamentarians’ Seminar – The Spread of Avian Influenza (Bird Flu): Prevention and Policy. . 2005.

Peiris J.S.M., Topic: Avian flue: the threat to human health., WHO Influenza Training Course. Sapporo, Japan. . 2004.

Poon L.L.M., Wong B.W., Chan K.H., Leung C.S., Yuen K.Y., Guan Y. and Peiris J.S.M., A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors., Journal of clinical virology. 2004, 30(3): 214-7.

Poon L.L.M., Leung C.S.W., Chan K.H., Lee H.C., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of human influenza A viruses by loop-mediated isothermal amplification., Journal of Clinical Microbiology. 2005, 43(1): 427-30.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Poon L.L.M., Guan Y., Nicholls J.M., Yuen K.Y. and Peiris J.S.M., The aetiology, origins, and diagnosis of severe acute respiratory syndrome., Lancet Infectious Diseases. 2004, 4(11): 663-71.

Ren Y., He Q., Fan J.Q., Jones B., Zhou Y., Xie Y., Cheung C.Y., Wu A.Y.Y., Chiu J., Peiris J.S.M. and Tam P.K.H., The use of proteomics in the discovery of serum biomarkers from patients with severe acute respiratory syndrome, Proteomics. 2004, 4: 3477-3484.

Samaranayake L.P. and Peiris J.S.M., Severe acute respiratory syndrome, Journal of the American Dental Association. 2004, 135: 1292-1302.

Thach T.Q., Wong C.M., Chan K.P., Chau Y.K., Hedley A.J. and Peiris J.S.M., The validity of utilizing respiratory mortality data for identification of influenza epidemics in air pollution time-series studies (Abstract and poster), Better Air Quality 2004, 6-8 December 2004, Agra, India. Agra, India, Clean Air Initiative, 2004, 35.

Webster R.G., Lipatov A.S., Govorkova E.A., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webby R., Evolution of H5N1 influenza viruses in Asia., Nova Acta Leopoldina. 2005, 344: 75-80.

Wong C.M., Chan K.P., Thach T.Q., Ou C., Hedley A.J. and Peiris J.S.M., A case-crossover study on health impact of influenza circulation measured by weekly proportion of positive influenza virus isolations (Abstract), Epidemiologypresented in 16th Conference of the International Society for Environmental Epidemiology, 1-4 August 2004, New York. 2004, 15(4): S191-S192.

Wong C.M., Chan K.P., Hedley A.J. and Peiris J.S.M., Influenza-associated mortality in Hong Kong, Clinical Infectious Diseases. 2004, 39: 1611-1617.

Wong C.M., Peiris J.S.M., Thach T.Q., Chau Y.K., Chan K.P., Thomas G.N., Lam T.H., Wong T.W. and Hedley A.J., Interaction between air polution and respiratory viruses: Time-series studies for daily mortality and hospital admissions (Abstract and poster), Better Air Quality 2004, 6-8 December 2004, Agra, India. Agra, India, Clean Air Initiative, 2004, 17.

Wong C.M., Peiris J.S.M., Thach T.Q., Chau Y.K., Chan K.P., Thomas G.N., Lam T.H., Wong T.W. and Hedley A.J., Time-series studies for daily mortality and hospital admissions: Interaction between air pollution and respiratory viruses (Abstract), Health Effects Institute Annual Conference 2005, 17-19 April 2005, Baltimore. Baltimore, Health Effects Institute, 2005, 41-42.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Chan K.H., Hui W.T., Kwan G.S., Peiris J.S.M., Couch R.B. and Yuen K.Y., False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide., Journal of clinical microbiology. 2004, 42(12): 5885-8.

Woo P.C.Y., Lau S.K.P., Wong B.H., Chan K.H., Chu C.M., Tsoi H.W., Huang Y., Peiris J.S.M. and Yuen K.Y., Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus, Clinical and diagnostic laboratory immunology. 2004, 11(4): 665-8.

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Yap F.H.Y., Ho P.L., Lam K.F., Chan K.S., Cheng Y.H. and Peiris J.S.M., Excess hospital admissions for pneumonia, chronic obstructive pulmonary diseases, and heart failure during influenza seasons in Hong Kong, Journal of Medical Virology. Wiley-Liss, Inc., 2004, 73: 617-623.

Zhou J., Law H.K.W., Cheung C.Y., Peiris J.S.M. and Lau Y.L., Upregulation of Death Receptor Ligands in Adult and Neonatal Macrophages by Avian Influenza Viruses: A Mechanism for the Unusual Severity of Human Diseases?, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 52.

Zhou J., Law H.K.W., Cheung C.Y., Peiris J.S.M. and Lau Y.L., Upregulation of Death Receptor Ligands in Human Antigen Presenting Cells by Avian Flu Viruses: a Mechanism for the Unusual Severity of Human Diseases?, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 113.

Researcher : Poon LLM

Project Title:	Reverse genetic for a coronavirus responsible for severe acute respiratory syndrome
Investigator(s):	Poon LLM, Guan Y
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To generate a coronavirus-like mini vRNA GFP (green fluorescent protein) reporter system to study the transcription and replication of coronavirus; to establish a reverse genetic system of coronavirus.

Project Title:	Rapid molecular diagnosis of SARS coronavirus
Investigator(s):	Poon LLM, Peiris JSM, Tam J.S.L., Lo D.Y.M.
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (Special Grants for Severe Acute Respiratory Syndrome Research)
Start Date:	08/2003
Completion Date:	07/2004

Abstract:

To refine existing PCR-based assays for SARS-CoV detection; to develop novel quantitative PCR-based assays which target at other regions in the SARS-CoV genome; to identify the optimal clinical samples for SARS-CoV diagnosis; to determine the quantity of various viral RNA species (i.e. genomic, subgenomic RNA and defective interference viral particle) in clinical samples; to apply the quantitative PCR assays to determine the viral dynamic in SARS patients at different time points; to quantitative the amount of SARS-CoV in atypical SARS patients, such as asymptomatic patients and 'super-spreaders'.

Project Title:	Roles of segment-specific vRNA sequences of influenza A virus in viral replication, transcription and gene expression
Investigator(s):	Poon LLM, Guan Y, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	11/2003

Abstract:

To establish a GFP reporter assay for influenza replication studies; to determine the potential function of segment-specific viral RNA sequences in viral replication, tansporation and gene expression; to study the 5' and 3' segment-specific regions of avian, human and swine influenza.

Project Title:	Roles of segment-specific vRNA sequences of influenza A virus in viral replication, transcription and gene expression
Investigator(s):	Poon LLM, Guan Y, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2003

Abstract:

Project Title:	Use of small molecules as antiviral drugs for influenza A viruses
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Seed Funding for New Staff
Start Date:	07/2004

Abstract:

The recent use of high-throughput screening (HTS) to search for small molecule inhibitors provides a new possibility of identifying small molecules with anti-influenza effects. This approach has been proved to be useful in finding small molecule inhibitors of angiogenin. We intend to apply HTS technology to identify small molecules to inhibit influenza virus replication. In particular, we intend to identify inhibitors that are specific for the viral polymerase subunits.

Project Title:	Splicing of influenza A virus
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2004

Abstract:

To study the sequence requirements for the splicing of influenza viral mRNA; to study the role of splicing in controlling viral protein expression; to generate recombinant influenza viruses with mutations in the splicing sequences and determine the global effects of mutations in these regions in an influenza life cycle; to test whether there is a host-specific selective pressure on the 5' splicing site of influenza mRNA.

Project Title:	Splicing of influenza A virus
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Chan K.H., Yau C.Y., Chu K.W., Poon L.L.M., Maldeis N.E. and Peiris J.S.M., Evaluation of Reverse Transcriptase-Strand Displacement Amplification (BD Probetec^TM) for Rapid Diagnosis of SARS Coronavirus Infection, 21st Annual Clinical Virology Symposium; Florida, USA; 8-9 May, 2005.. 2005.

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Cheung K.W., Guan Y., Ng S.F., Chen H., Wong C.H.K., Peiris J.S.M. and Poon L.L.M., Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron., Journal of General Virology. 2005, 86(Pt 5): 1447-54.

Chiu S.S.S., Chan K.H., Chu K.W., Kwan S.W., Guan Y., Poon L.L.M. and Peiris J.S.M., Human Coronavirus NL63 Infection and Other Coronavirus Infections in Children Hospitalized with Acute Respiratory Disease in Hong Kong, China, Clinical Infectious Diseases. 2005, 40: 172-11729.

Choi Y.K., Ozaki H., Webby R.J., Webster R.G., Peiris J.S.M., Poon L.L.M., Butt C., Leung C.Y.H. and Guan Y., Continuing evolution of H9N2 influenza viruses in Southeastern China., Journal of virology. 2004, 78(16): 8609-14.

Chu C.M., Poon L.L.M., Cheng V.C.C., Chan K.S., Hung I.F., Wong M.M., Chan K.H., Leung W.S., Tang S.F., Chan V.L., Ng W.L., Sim T.C., Ng P.W., Law K.I., Tse D.M., Peiris J.S.M. and Yuen K.Y., Initial viral load and the outcomes of SARS., Canadian Medical Association journal . 2004, 171(11): 1349-52.

Chye M.L., Li H., Ramalingam S., Poon L.L.M. and Peiris J.S.M., Genetically modified plants comprising SARS-CoV viral nucleotide sequences and methods of use thereof for immunization against SARS, US Patent Application No. 11/004,399 filed December 3, 2004 [Patent WO2005054473-A1 published June 16,2005] . United States Patent Office, 2005.

Han D.P., Kim H.G., Kim Y.B., Poon L.L.M. and Cho M.W., Development of a safe neutralization assay for SARS-CoV and characterization of S-glycoprotein., Virology. 2004, 326 (1): 140-9.

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Lam E., Poon L.L.M., Zhou Y., Chye M.L. and Peiris J.S.M., Transgenic plant-derived siRNAs: suppression of influenza virus propagation in mammalian cells, US Provisional Patent Application No. 60/673,100 filed April 20, 2005 . 2005.

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Lipatov A.S., Govorkova E.A., Webby R.J., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webster R.G., Influenza: emergence and control., Journal of virology. 2004, 2004: 8951-9.

Poon L.L.M., Wong B.W., Chan K.H., Leung C.S., Yuen K.Y., Guan Y. and Peiris J.S.M., A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors., Journal of clinical virology. 2004, 30(3): 214-7.

Poon L.L.M., Animal/Avian influenza and pandemic disease, “Moving Towards Prevention and Control of Influenza Pandemic in the Next Decade”, Bangkok, Thailand.. 2004.

Poon L.L.M., Animal/Avian influenza and pandemic disease., in a Conference “Moving Towards Prevention and Control of Influenza Pandemic in the Next Decade”, Bangkok, Thailand.. 2004.

Poon L.L.M., Leung C.S.W., Chan K.H., Lee H.C., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of human influenza A viruses by loop-mediated isothermal amplification., Journal of Clinical Microbiology. 2005, 43(1): 427-30.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Poon L.L.M., SARS and other emerging viral infections in South East Asia, “Emerging infectious viral diseases in South East Asia”, Hanoi, Vietnam.. 2005.

Poon L.L.M., SARS etiology, transmission and diagnostic., in International symposium on Molecular aspects and prevent of SARS, Madrid, Spain.. 2004.

Poon L.L.M., SARS: aetiology, transmission and diagnosis, “Molecular aspects and prevention of SARS”, Madrid, Spain.. 2004.

Poon L.L.M., Sars and other coronavirus in humans and animals, Xth International Symposium of Nidoviruses, Colorado, USA.. 2005.

Poon L.L.M., Specific threat: emerge of human H5N1 of pandemic potential?, “Emergence and control of viral respiratory disease”, Foundation Merieux Conference Centre, France.. 2004.

Poon L.L.M., Guan Y., Nicholls J.M., Yuen K.Y. and Peiris J.S.M., The aetiology, origins, and diagnosis of severe acute respiratory syndrome., Lancet Infectious Diseases. 2004, 4(11): 663-71.

Poon L.L.M., Top 1% of most cited scientists (Clinical Medicine), ISI Web of Knowledge. 2005.

Webster R.G., Lipatov A.S., Govorkova E.A., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webby R., Evolution of H5N1 influenza viruses in Asia., Nova Acta Leopoldina. 2005, 344: 75-80.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Zheng B., He M.L., Wong K.L., Lum C.T., Poon L.L.M., Peng Y., Guan Y., Lin M.C. and Kung H., Potent inhibition of SARS-associated coronavirus (SCoV) infection and replication by type I interferons (IFN-alpha/beta)but not by type II interferon (IFN-gamma), Journal of Interferon and Cytokine Research. 2004, 24(7): 388-90.

Researcher : Poon RWS

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Researcher : Qu D

List of Research Outputs

Qu D., Zheng B., Yao X., Guan Y., Yuan Z.H., Zhong N.S., Lu L., Xie J. and Wen Y., Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) in mice induced local and serum antibodies., Vaccine. 2005, 23: 924-931.

Researcher : Seto WH

List of Research Outputs

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Yam W.C., Tam C.M., Leung C.C., Chan K.H., Leung T.Y., Wong K.C., Yew W.W., Seto W.H., Yuen K.Y. and Ho P.L., Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA sequencing., J Clin Microbiol . USA, ASM, 2004, 42: 4438-4443.

Researcher : Shiu SYW

Project Title:	Role of PDZD2 in the modulation of prostate cancer cell proliferation
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To determine the expression of full-length and secreted forms of PDZD2 in common prostate cancer cell lines; to detect any changes in the expression of the full-length and secreted forms of PDZD2 upon hormonal stimulation of prostate cancer cell proliferation; to study the effect of decreased endogenous expression of PDZD2 on prostate cancer cell proliferation.

Project Title:	sPDZD2-induced protein profile changes in prostate cancer
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To express and purify sPDZD2 in E. coli, and to study the effects of the recombinant protein on the protein profiles of human prostate cancer to cells using a 'proteomics' approach.

Researcher : Shortridge KF

List of Research Outputs

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Researcher : Sia SF

List of Research Outputs

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells, Blood. 2005, 106(7): 2366-2374.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine upregulation in SARS coronavirus infected human monocyte derived dendritic cells, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 85.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Human Monocyte Derived Dendritic Cells Infected by SARS Associated Coronavirus, 12th International Congress of Immunology and 4th Annual Conference of FOCIS, Montreal, Canada, 18-23 July 2004.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Peiris J.S.M. and Lau Y.L., SARS Coronavirus Induced Chemokines Expression in Human Monocyte Derived Dendritic Cells, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 48.

Researcher : Smith GJ

List of Research Outputs

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Researcher : Tang SF

List of Research Outputs

Cheng V.C.C., Tang S.F., Wu A.K., Chu C.M. and Yuen K.Y., Medical treatment of viral pneumonia including SARS in immunocompetent adult., The Journal of infection. 2004, 49(4): 262-73.

Chu C.M., Poon L.L.M., Cheng V.C.C., Chan K.S., Hung I.F., Wong M.M., Chan K.H., Leung W.S., Tang S.F., Chan V.L., Ng W.L., Sim T.C., Ng P.W., Law K.I., Tse D.M., Peiris J.S.M. and Yuen K.Y., Initial viral load and the outcomes of SARS., Canadian Medical Association journal . 2004, 171(11): 1349-52.

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Ng K.H., Wu A.K., Cheng V.C.C., Tang S.F., Chan C.Y., Yung C.Y., Luk S.H., Lee T.W., Chow L. and Yuen K.Y., Pulmonary artery thrombosis in a patient with severe acute respiratory syndrome., Postgraduate medical journal. 2005, 81(956): e3.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M., Tang S.F. and Yuen K.Y., Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing., Journal of clinical pathology. 2005, 58(3): 301-7.

Wu A.K., Cheng V.C.C., Tang S.F., Hung I.F., Lee R.A., Hui D.S. and Yuen K.Y., The unmasking of Pneumocystis jiroveci pneumonia during reversal of immunosuppression: case reports and literature review., BMC infectious diseases. 2004, 4(1): 57.

Researcher : Teng LL

List of Research Outputs

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Eco-epidemiology of Laribacter Hongkongensis, a novel bacterium associated with gastroenteritis, 9th Research Postgraduate Symposium., 2004.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H., Ng L.T., Hui W.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis., Journal of clinical microbiology. 2005, 43(2): 919-22.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia., Microbiology and immunology. 2005, 49(1): 31-9.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Wong M.K.M. and Yuen K.Y., Bacteremia in a patient with colonic carcinoma caused by a novel Sedimentibacter species: Sedimentibacter hongkongensis sp. nov., Diagnostic microbiology and infectious disease. 2004, 50(2): 81-7.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Tse H., Wong B.H.L. and Yuen K.Y., Streptococcus sinensis may react with Lancefield group F antiserum., Journal of medical microbiology. 2004, 53(Pt 11): 1083-8.

Researcher : Teng LL

List of Research Outputs

Researcher : To APC

List of Research Outputs

Kao R.Y.T., To A.P.C., Ng L.W.Y., Tsui W.H.W., Lee T.S.W., Tsoi H.W. and Yuen K.Y., Characterization of SARS-CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence-based assay., FEBS letters. 2004, 576(3): 325-30.

Researcher : To PCA

List of Research Outputs

Lau S.K.P., Woo P.C.Y., To P.C.A., Lau T.Y. and Yuen K.Y., Lack of evidence that DNA in antibiotic preparations is a source of antibiotic resistance genes in bacteria from animal or human sources., Antimicrob Agents Chemother. 2004, 48(8): 3141-6.

Woo P.C.Y., To P.C.A., Lau S.K.P., Fung A.M. and Yuen K.Y., Phenotypic and molecular characterization of erythromycin resistance in four isolates of Streptococcus-like gram-positive cocci causing bacteremia., Journal of clinical microbiology. 2004, 2004: 3303-5.

Researcher : Tse H

List of Research Outputs

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess., Systematic and applied microbiology. 2005, 28(4): 316-22.

Researcher : Tsoi HW

List of Research Outputs

Kao R.Y.T., To A.P.C., Ng L.W.Y., Tsui W.H.W., Lee T.S.W., Tsoi H.W. and Yuen K.Y., Characterization of SARS-CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence-based assay., FEBS letters. 2004, 576(3): 325-30.

Lau S.K.P., Ho P.L., Li M.W., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and characterization of a chromosomal class C beta-lactamase and its regulatory gene in Laribacter hongkongensis., Antimicrobial agents and chemotherapy. 2005, 49(5): 1957-64.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Lau S.K.P., Wong B.H., Chan K.H., Chu C.M., Tsoi H.W., Huang Y., Peiris J.S.M. and Yuen K.Y., Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus, Clinical and diagnostic laboratory immunology. 2004, 11(4): 665-8.

Researcher : Tsui WHW

List of Research Outputs

Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75.

Kao R.Y.T., To A.P.C., Ng L.W.Y., Tsui W.H.W., Lee T.S.W., Tsoi H.W. and Yuen K.Y., Characterization of SARS-CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence-based assay., FEBS letters. 2004, 576(3): 325-30.

Researcher : Wang J

List of Research Outputs

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Researcher : Wang TKF

List of Research Outputs

Wang T.K.F., Ho P.L. and Wong S.S.Y., Recurrent Salmonella bacteraemia caused by different Salmonella species in a systemic lupus erythematosus patient. , New Microbiologica. 2005, 28: 151-156.

Researcher : Wang Y

List of Research Outputs

Che X., Hao W., Wang Y., Di B., Yin K., Xu Y.C., Feng C.S., Wan Z.Y., Cheng V.C.C. and Yuen K.Y., Nucleocapsid protein as early diagnostic marker for SARS., Emerging infectious diseases. 2004, 10(11): 1947-9.

Di B., Hao W., Gao Y., Wang M., Wang Y., Qiu L.W., Wen K., Zhou D.H., Wu X.W., Lu E.J., Liao Z.Y., Mei Y.B., Zheng B. and Che X.Y., Monoclonal Antibody-based Antigen Capture Enzyme-linked Immunosorbent Assay Reveals High Sensitivity Of The Nucleocapsid Protein In Acute-phase Sera Of Severe Acute Respiratory Syndrome Patients., Clinical and Diagnostic Laboratory Immunology. 2005, 12: 135-140.

Researcher : Webster RG

List of Research Outputs

Choi Y.K., Ozaki H., Webby R.J., Webster R.G., Peiris J.S.M., Poon L.L.M., Butt C., Leung C.Y.H. and Guan Y., Continuing evolution of H9N2 influenza viruses in Southeastern China., Journal of virology. 2004, 78(16): 8609-14.

Govorkova E.A., Rehg J.E., Krauss S., Yen H.L., Guan Y., Peiris J.S.M., Nguyen T.D., Hanh T.H., Puthavathana P., Long H.T., Buranathai C., Lim W., Webster R.G. and Hoffmann E., Lethality to ferrets of H5N1 influenza viruses isolated from humans and poultry in 2004., Journal of Virology. 2005, 79(4): 2191-8.

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Lipatov A.S., Govorkova E.A., Webby R.J., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webster R.G., Influenza: emergence and control., Journal of virology. 2004, 2004: 8951-9.

Webster R.G., Lipatov A.S., Govorkova E.A., Ozaki H., Peiris J.S.M., Guan Y., Poon L.L.M. and Webby R., Evolution of H5N1 influenza viruses in Asia., Nova Acta Leopoldina. 2005, 344: 75-80.

Researcher : Wen Y

List of Research Outputs

Qu D., Zheng B., Yao X., Guan Y., Yuan Z.H., Zhong N.S., Lu L., Xie J. and Wen Y., Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) in mice induced local and serum antibodies., Vaccine. 2005, 23: 924-931.

Researcher : Wong BCW

List of Research Outputs

Wong B.C.W., Essentiality of Methionine Aminopeptidase in Staphyloccccus aureus (MPhil thesis), supervisor: Kao R.Y.T., Yuen K.Y., 2005.

Researcher : Wong BHL

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Chan K.H., Hui W.T., Kwan G.S., Peiris J.S.M., Couch R.B. and Yuen K.Y., False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide., Journal of clinical microbiology. 2004, 42(12): 5885-8.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Tse H., Wong B.H.L. and Yuen K.Y., Streptococcus sinensis may react with Lancefield group F antiserum., Journal of medical microbiology. 2004, 53(Pt 11): 1083-8.

Researcher : Wong CHK

List of Research Outputs

Cheung K.W., Guan Y., Ng S.F., Chen H., Wong C.H.K., Peiris J.S.M. and Poon L.L.M., Generation of recombinant influenza A virus without M2 ion-channel protein by introduction of a point mutation at the 5' end of the viral intron., Journal of General Virology. 2005, 86(Pt 5): 1447-54.

Researcher : Wong KC

List of Research Outputs

Yam W.C., Tam C.M., Leung C.C., Chan K.H., Leung T.Y., Wong K.C., Yew W.W., Seto W.H., Yuen K.Y. and Ho P.L., Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA sequencing., J Clin Microbiol . USA, ASM, 2004, 42: 4438-4443.

Researcher : Wong KH

List of Research Outputs

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zheng B., Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ma S. and Ng M.H., Effects of single nucleotide polymorphism in the RANTES promoter region on HIV infection and disease progression in indigenous Chinese, XV international AIDS conference. 11-16 July, 2004. Bangkok, Thailand.. 2004.

Researcher : Wong KL

List of Research Outputs

He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.

Zheng B., He M.L., Wong K.L., Lum C.T., Poon L.L.M., Peng Y., Guan Y., Lin M.C. and Kung H., Potent inhibition of SARS-associated coronavirus (SCoV) infection and replication by type I interferons (IFN-alpha/beta)but not by type II interferon (IFN-gamma), Journal of Interferon and Cytokine Research. 2004, 24(7): 388-90.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Wong LP

List of Research Outputs

Leung A.Y.H., Chan M.T.L., Yuen K.Y., Cheng V.C.C., Chan K.H., Wong L.P., Liang R.H.S., Lie A.K.W. and Kwong Y.L., Ciprofloxacin Decreased Polyoma BK Virus Load in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation, Clinical Infectious Disease. 2005, 40: 528-537.

Researcher : Wong MKM

List of Research Outputs

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess., Systematic and applied microbiology. 2005, 28(4): 316-22.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia., Microbiology and immunology. 2005, 49(1): 31-9.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Wong M.K.M. and Yuen K.Y., Bacteremia in a patient with colonic carcinoma caused by a novel Sedimentibacter species: Sedimentibacter hongkongensis sp. nov., Diagnostic microbiology and infectious disease. 2004, 50(2): 81-7.

Researcher : Wong OK

List of Research Outputs

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Researcher : Wong SSY

Project Title:	A novel method of bacterial identification: groEL gene sequencing for discriminating Burkholderia pseudomallei and Burkholderia thailandensis
Investigator(s):	Wong SSY, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To clone the groEL gene of B. thailandensis; to examine the potential of using this gene target for discriminating between B. thailandensis and B. pseudomallei.

Project Title:	Molecular characterization of streptococcus bovis bacteraemia
Investigator(s):	Wong SSY, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To characterize 48 S. bovis strains isolated from the blood cultures of 37 bacteraemic patients over a six-year period using a combination of phenotypic and genotypic techniques. The epidemiology, underlying diseases, clinical disease associations, and outcome of S. bovis bacteraemia in relation to the different biotypes and genotypes of S. bovis will be investigated, and the molecular epidemiology of erythromycin resistance in the S. bovis strains will also be analyzed.

Project Title:	Screening antigenic proteins in penicillium marneffei for seroepidemiology and vaccination
Investigator(s):	Wong SSY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

To screen for yeast- and mould-phase-specific antigens that are useful for epidemiological and diagnostic purposes; to screen for yeast-phase- and mould-phase-specific antigens that induce active immunity against penicilliosis marneffei from normalized cDNA libraries of the two phases of the dimorphic fungus; to construct a prophylactic and/or therapeutic vaccine prototype; to study the seroepidemiology of penicilliosis marneffei.

Project Title:	Screening antigenic proteins in penicillium marneffei for seroepidemiology and vaccination
Investigator(s):	Wong SSY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

Project Title:	Characterization of bacterial isolates with ambiguous biochemical profiles recovered from patients with clinically significant bacteremia by 16S rRNA gene sequencing, the state-of-the-art molecular technique for bacterial identification
Investigator(s):	Wong SSY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To use the state-of-the-art molecular technique for bacterial identification, 16S rRNA gene sequencing, to characterize these bacterial isolates. Novel bacterial species discovered will be further characterized.

List of Research Outputs

Chim J.C.S., Wong S.S.Y., Lam C.C.K. and Chan K.W., Concurrent hyperreactive malarial splenomegaly and quartan malarial nephropathy – Plasmodium malariae revisited, Haematologica. 2004, 89(7): ECR21.

Chow C.K., Wong S.S.Y., Ho A.C.W. and Lau S.K.P., Unilateral epistaxis after swimming in a stream., Hong Kong Medical Journal. 2005, 11: 110-112.

Ho P.L., Lam K.F., Chow K.H., Lau Y.L., Wong S.S.Y., Cheng S.L.E. and Chiu S.S.S., Serotype distribution and antimicrobial resistance patterns of nasopharyngeal and invasive Streptococcus pneumoniae isolates in Hong Kong children., Vaccine. 2004, 22(25-26): 3334-9.

Wang T.K.F., Ho P.L. and Wong S.S.Y., Recurrent Salmonella bacteraemia caused by different Salmonella species in a systemic lupus erythematosus patient. , New Microbiologica. 2005, 28: 151-156.

Wong S.S.Y. and Yuen K.Y., Human infection by avian influenza A H5N1., Hong Kong Medical Journal. Hong Kong, Hong Kong Academy of Medicine, 2005, 11: 189-199.

Wong S.S.Y. and Yuen K.Y., Penicilliosis in China, Mycopathologia. 2004, 158: 147-150.

Wong S.S.Y. and Yuen K.Y., Red imported fire ants in Hong Kong, Hong Kong Medical Journal. Hong Kong, Hong Kong Academy of Medicine, 2005, 11: 131-132.

Wong S.S.Y., Woo P.C.Y. and Yuen K.Y., Unusual laboratory findings in a case of Norwegian scabies provided a clue to diagnosis, Journal of Clinical Microbiology. 2005, 43: 2542-2544.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., Continuous ambulatory peritoneal dialysis-related peritonitis associated with Lancefield group G beta-hemolytic streptococcus: report of two cases requiring Tenckhoff catheter removal., Journal of clinical microbiology. 2004, 42(9): 4399-402.

Woo P.C.Y., Lau S.K.P., Lau C.C., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient., Bone marrow transplantation. 2005, 35(8): 831-3.

Researcher : Woo GKS

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Fung A.M., Chan K.M., Woo G.K.S. and Yuen K.Y., Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing., Journal of medical microbiology. 2004, 53(Pt 12): 1247-53.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Lau S.K.P., Woo P.C.Y., Chan C.Y., Wu W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi., Journal of clinical microbiology. 2005, 43(3): 1470-2.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess., Systematic and applied microbiology. 2005, 28(4): 316-22.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia., Microbiology and immunology. 2005, 49(1): 31-9.

Woo P.C.Y., Lau S.K.P., Woo G.K.S., Fung A.M., Yiu V.P. and Yuen K.Y., Bacteremia due to Clostridium hathewayi in a patient with acute appendicitis., Journal of clinical microbiology. 2004, 42(12): 5947-9.

Researcher : Woo PCY

Project Title:	A novel gold standard of bacterial identification: granulicatella adiacens and abiotrophia defectiva bacteremia characterized by 16S ribosomal RNA gene sequencing
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To use 16S rRNA gene sequencing, assisted by traditional phenotypic tests; to define the epidemiology, clinical diseases, and outcome of G. adiacens and A. defectiva bacteremia.

Project Title:	Development of an ELISA-based antibody test for SARS associated coronavirus infection
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To develop and evaluate an ELISA based on the highly immunogenic nucleocapsid protein of SARS associated coronavirus; to examine the seroprevalence of the general population before and after the SARS outbreak and non-pneumonic SARS associated coronavirus infections in both the general population and health care workers.

Project Title:	Epidemiology and pathogenesis of a novel clinical syndrome: laribacter hongkongensis gastroenteritis
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To study the epidemiology of L. hongkongensis gastroenteritis in humans; to provide clinicians with appropriate treatment regimen by correlation of antibiotic susceptibilities and clinical outcome; to discover the source and reservoir of the bacterium; to define its pathogenesis in terms of toxin production, tissue and animal invasiveness.

Project Title:	Molecular epidemiology of erythromycin resistant [beta]-hemolytic Lancefield group G streptococci causing bacteremia
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To perform phenotypic and genotypic characterization of the erythromycin resistance in [beta]-hemolytic group G streptococci recovered from blood cultures of patients in a six-year period. Risk factors for bacteremia caused by [beta]-hemolytic group G streptocci will also be analyzed.

Project Title:	The origin of antibiotic resistance genes: a genome based phylogentic analysis
Investigator(s):	Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To perform a genome based in silico analysis to determine the reason for antibiotic resistance genes in actinomycetes being the "origins" of some antibiotic resistance genes.

Project Title:	The role of a novel bacterium, Laribacter hongkongensis, in gastroenteritis, and its epidemiology and pathogenesis
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To study the association of L. hongkongensis with gastroenteritis in humans and the epidemiology of this novel disease entity; to provide clinicians with appropriate treatment regimen by correlation of antibiotic susceptibilities and clinical outcome; to discover the source and reservoir of the bacterium; to define its pathogenesis in terms of toxin production, tissue and animal invasiveness.

Project Title:	The role of a novel bacterium, Laribacter hongkongensis, in gastroenteritis, and its epidemiology and pathogenesis
Investigator(s):	Woo PCY
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Lau S.K.P., Woo P.C.Y., Fung A.M., Chan K.M., Woo G.K.S. and Yuen K.Y., Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing., Journal of medical microbiology. 2004, 53(Pt 12): 1247-53.

Lau S.K.P., Ho P.L., Li M.W., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and characterization of a chromosomal class C beta-lactamase and its regulatory gene in Laribacter hongkongensis., Antimicrobial agents and chemotherapy. 2005, 49(5): 1957-64.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Lau S.K.P., Woo P.C.Y., To P.C.A., Lau T.Y. and Yuen K.Y., Lack of evidence that DNA in antibiotic preparations is a source of antibiotic resistance genes in bacteria from animal or human sources., Antimicrob Agents Chemother. 2004, 48(8): 3141-6.

Lau S.K.P., Woo P.C.Y., Chan C.Y., Wu W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi., Journal of clinical microbiology. 2005, 43(3): 1470-2.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Eco-epidemiology of Laribacter Hongkongensis, a novel bacterium associated with gastroenteritis, 9th Research Postgraduate Symposium., 2004.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H., Ng L.T., Hui W.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis., Journal of clinical microbiology. 2005, 43(2): 919-22.

Wong S.S.Y., Woo P.C.Y. and Yuen K.Y., Unusual laboratory findings in a case of Norwegian scabies provided a clue to diagnosis, Journal of Clinical Microbiology. 2005, 43: 2542-2544.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., A novel coronavirus, HKU1, from patients with pneumonia, 15th European Congress of Clinical Microbiology and Infectious Diseases. 2005.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess., Systematic and applied microbiology. 2005, 28(4): 316-22.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia., Microbiology and immunology. 2005, 49(1): 31-9.

Woo P.C.Y., Lau S.K.P., Woo G.K.S., Fung A.M., Yiu V.P. and Yuen K.Y., Bacteremia due to Clostridium hathewayi in a patient with acute appendicitis., Journal of clinical microbiology. 2004, 42(12): 5947-9.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Wong M.K.M. and Yuen K.Y., Bacteremia in a patient with colonic carcinoma caused by a novel Sedimentibacter species: Sedimentibacter hongkongensis sp. nov., Diagnostic microbiology and infectious disease. 2004, 50(2): 81-7.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Biliary tract disease as a risk factor for Plesiomonas shigelloides bacteraemia: a nine-year experience in a Hong Kong hospital and review of the literature., The new microbiologica. 2005, 28(1): 45-55.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M., Tang S.F. and Yuen K.Y., Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing., Journal of clinical pathology. 2005, 58(3): 301-7.

Woo P.C.Y., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., Continuous ambulatory peritoneal dialysis-related peritonitis associated with Lancefield group G beta-hemolytic streptococcus: report of two cases requiring Tenckhoff catheter removal., Journal of clinical microbiology. 2004, 42(9): 4399-402.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Chan K.H., Hui W.T., Kwan G.S., Peiris J.S.M., Couch R.B. and Yuen K.Y., False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide., Journal of clinical microbiology. 2004, 42(12): 5885-8.

Woo P.C.Y., Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, Hong Kong Institute of Medical Laboratory Sciences. 2004.

Woo P.C.Y., Lau S.K.P., Wong B.H., Chan K.H., Chu C.M., Tsoi H.W., Huang Y., Peiris J.S.M. and Yuen K.Y., Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus, Clinical and diagnostic laboratory immunology. 2004, 11(4): 665-8.

Woo P.C.Y., New emerging infectious diseases, XXI International Seminar on Clinical Microbiology. 2004.

Woo P.C.Y., Lau S.K.P., Lau C.C., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient., Bone marrow transplantation. 2005, 35(8): 831-3.

Woo P.C.Y., To P.C.A., Lau S.K.P., Fung A.M. and Yuen K.Y., Phenotypic and molecular characterization of erythromycin resistance in four isolates of Streptococcus-like gram-positive cocci causing bacteremia., Journal of clinical microbiology. 2004, 2004: 3303-5.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Tse H., Wong B.H.L. and Yuen K.Y., Streptococcus sinensis may react with Lancefield group F antiserum., Journal of medical microbiology. 2004, 53(Pt 11): 1083-8.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Wu AKL

List of Research Outputs

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Researcher : Wu HS

List of Research Outputs

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Researcher : Wu WL

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Chan C.Y., Wu W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi., Journal of clinical microbiology. 2005, 43(3): 1470-2.

Researcher : Wu Y

List of Research Outputs

Chen H., Huang J., Wu Y., Liao G., Hutt-Fletcher L. and Hayward S.D., Chen H, Huang J, Wu FY, Liao G, Hutt-Fletcher L, Hayward SD. Related Articles, Links Regulation of expression of the Epstein-Barr virus BamHI-A rightward transcripts., Journal of Virology. 2005, 79(3): 1724-33.

Researcher : Xie J

List of Research Outputs

Qu D., Zheng B., Yao X., Guan Y., Yuan Z.H., Zhong N.S., Lu L., Xie J. and Wen Y., Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) in mice induced local and serum antibodies., Vaccine. 2005, 23: 924-931.

Researcher : Xu K

List of Research Outputs

Li W.S., Zhang C., Sui J., Kuhn H., Moore M.J., Luo S., Wong S.K., Huang I.C., Xu K., Vasilieva N., Murakami A., He Y., Marasco W.A., Guan Y., Choe H. and Farzan M., Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. , The EMBO journal. 2005, 24(8): 1634-1643.

Researcher : Yam WC

List of Research Outputs

Ching W.H., Leung M.K.H., Leung Y.C., Yam W.C., Ng S.P. and Vrijmoed L.L.P., Photocatalytic degradation of gaseous formaldehyde by Sol-Gel TiO₂ thin film coated on glass, Proceedings of 2004 Asia-Pacific Conference of International Solar Energy Society (ISES). Gwangju, Korea, 2004, 561-567.

Fung Y.S., Sun H., Zhang Y., Yao S. and Yam W.C., PCR/DNA piezoelectric quartz crystal biosensor for bacteria sensing, Abstract of 2^nd France China Workshop on Surface Molecules of Biological Interest: Biosensor Applications, October 13-16, 2004, Céret, France, p 36. 2004, 36.

Fung Y.S., Sun H., Zhang Y., Yao S.Z. and Yam W.C., PCR/DNA piezoelectric quartz crystal biosensor for bacteria sensing, Invited lecture at Plenary Session of 2^nd France China Workshop on Surface Molecules of Biological Interest: Biosensor Application, Céret, France, October 13-16. 2004.

Ho P.L., Shek R.H., Chow K.H., Duan R., Mak C.K., Lai E.L.Y., Yam W.C., Tsang K.W.T. and Lai W.M., Detection and characterization of extended-spectrum beta-lactamases among bloodstream isolates of Enterobacter spp. in Hong Kong, 2000-2002., Jouranl of Antimicrobial Chemotherapy. 2005, 55(3): 326-32.

Ho P.L., Ho A.Y., Chow K.H., Wong R.C., Duan R.S., Ho W.L., Mak G.C., Tsang K.W., Yam W.C. and Yuen K.Y., Occurrence and molecular analysis of extended-spectrum {beta}-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002., The Journal of antimicrobial chemotherapy. 2005, 55(6): 840-5.

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Yam W.C., Tam C.M., Leung C.C., Chan K.H., Leung T.Y., Wong K.C., Yew W.W., Seto W.H., Yuen K.Y. and Ho P.L., Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA sequencing., J Clin Microbiol . USA, ASM, 2004, 42: 4438-4443.

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zhao X.Y., Lee S.S., Wong K.H., Chan K.C., Ma S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Effects of single nucleotide polymorphisms in the RANTES promoter region in healthy and HIV-infected indigenous Chinese., European journal of immunogenetics. 2004, 31(4): 179-83.

Researcher : Yao X

List of Research Outputs

Qu D., Zheng B., Yao X., Guan Y., Yuan Z.H., Zhong N.S., Lu L., Xie J. and Wen Y., Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) in mice induced local and serum antibodies., Vaccine. 2005, 23: 924-931.

Researcher : Yap YL

List of Research Outputs

Yap Y.L., Zhang X., Ling M.T., Wang X., Wong Y.C. and Danchin A.L.M., Classification between normal and tumor tissues based on the pair-wise gene expression ratio, BMC Cancer . 2004, 4: 72.

Yap Y.L., Lam C.L., Girard L., Zhang X., Hernandez D., Gras R., Wang E., Chiu S. .W., Chung L.P., Lam W.K., Smith D.K., Minna J. .D., Danchin A. and Wong M.P., Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays, Nucleic Acids Research. Oxford University Press 2005, 2004, 33 No. 8: 2764.

Zhang X., Yap Y.L. and Danchin A.L.M., A Testing the hypothesis of a recombinant origin of the SARS-associated coronavirus., Achieves of Virology. Austria, 2004, 150: 1-20.

Zhang X., Yap Y.L. and Altmeyer R.M., Generation of predictive pharmacophore model for SARS-Coronavirus main proteinase, European Journal of Medicinal Chemistry. 2004, 40: 57-62.

Zhang X. and Yap Y.L., Putative structure and function of ORF3 in SARS coronavirus, Journal of Molecular Structure. Elsevier B V, 2004, 715: 55-58.

Zhang X. and Yap Y.L., The 3D structure analysis of SARS-CoV S1 protein reveals a link to influenza virus neuraminidase and implications for drug and antibody discovery., Journal of Molecular Structure (THEOCHEM). 2004, 681: 137-141.

Researcher : Yew WW

List of Research Outputs

Yam W.C., Tam C.M., Leung C.C., Chan K.H., Leung T.Y., Wong K.C., Yew W.W., Seto W.H., Yuen K.Y. and Ho P.L., Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA sequencing., J Clin Microbiol . USA, ASM, 2004, 42: 4438-4443.

Researcher : Yuen KY

Project Title:	Establishment of core facilities for the HKU HIV/AIDS research laboratories
Investigator(s):	Yuen KY, Zheng B, Woo PCY, Yam WC, Im SWK
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	11/2001

Abstract:

To support the development of state-of-art clinical management of HIV/AIDS founded on scientific research and clinical trials; to undertake research for expanding the knowledge base on HIV molecular epidemiology in Hong Kong and the reigon; to examine the immunological mechanisms underlying HIV pathogenesis; to study AIDS associated infectious diseases; to build expertise in HIV molecular biology and immunology.

Project Title:	Molecular characterization of a novel type of "Viridans Streptococcus"
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To report the isolation of a catalase negative, [alpha]-hemolytic, optochin and bacitracin resistant "viridans streptococcus" that does not grow in 10% or 40% bile, on MacConkey agar or bile esculin agar, or in 6% NaC1, from the blood culture of a 73-year old woman with pyomyositis and post-streptococcal reactive arthritis.

Project Title:	An oral DNA vaccine for SARS associated coronavirus infections
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To perform a pilot study to test the feasibility of using the live-attenuated Salmonella system for delivery of DNA vaccine of the nucleocapsid protein of SARS associated coronavirus in mice, and compare the immunogenicity of oral DNA vaccine, naked DNA vaccine, and recombinant protein vaccine.

Project Title:	Molecular characterization of Streptococcus milleri causing infective endocarditis
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To identify members of the "S. milleri group" in patients with infective endocarditis by 16S rRNA gene sequencing; to define the epidemiology, clinical spectrum, treatment, and outcome of infective endocarditis associated with the "S. milleri group".

Project Title:	An oral mucosal DNA vaccine for SARS coronavirus infections
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	06/2004

Abstract:

Refer to hard copy

Project Title:	A study of dendritic cell-based vaccine for immune therapy of chronic Hepatitis B virus infection
Investigator(s):	Yuen KY
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

Project Title:	Characterization of a novel bacterial genus and species, Anaerospora hongkongensis gen. nov.sp.nov., isolated from the blood culture of a patient
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To characterize a novel bacterial genus and species using various phenotypic and genotypic techniques; to understad its evolutional relationship to other related bacterial genus and species.

Project Title:	Characterisation of local/emerging pathogens and development of anti-infective agents
Investigator(s):	Yuen KY, Jin LJ, Kao RYT, Lau G, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Seed Funding for Strategic Research Theme
Start Date:	05/2005

Abstract:

There are 4 categories of pathogens are selected to compete with the rest of the world and therefore advance the research of our University to top international level: hepatitis B virus (HBV), Laribacter Hongkongenesis, influenze A virus and novel oral pathogens.

List of Research Outputs

Cai J., Smith D.K., Xia X. and Yuen K.Y., MBEToolbox: a MATLAB toolbox for sequence data analysis in molecular biology and evolution., BMC bioinformatics. 2005, 6(1): 64.

Che X., Hao W., Wang Y., Di B., Yin K., Xu Y.C., Feng C.S., Wan Z.Y., Cheng V.C.C. and Yuen K.Y., Nucleocapsid protein as early diagnostic marker for SARS., Emerging infectious diseases. 2004, 10(11): 1947-9.

Che X.Y., Qiu L.W., Liao Z.Y., Wang Y.D., Wen K., Pan Y.X., Hao W., Mei Y.B., Cheng V.C.C. and Yuen K.Y., Antigenic cross-reactivity between severe acute respiratory syndrome-associated coronavirus and human coronaviruses 229E and OC43., The Journal of infectious diseases. 2005, 191(12): 2033-7.

Chen S.F., Chan K.H., Jiang Y., Kao R.Y.T., Lu H., Fan K.W., Cheng V.C.C., Tsui W.H.W., Lee T.S.W., Peiris J.S.M. and Yuen K.Y., In vitro susceptibility of ten clinical isolates of SARS coronavirus to selected antiviral compounds, Journal of Clinical Virology. 2004, 31(1): 69-75.

Chen Z., Zhang L., Qin C., Ba L., Yi C.E., Zhang F., Wei Q., He T., Wu W., Wu J., Gao H., Tu X., Gettie A., Farzan M., Yuen K.Y. and Ho D.D., Recombinant modified vaccinia virus Ankara expressing the spike glycoprotein of severe acute respiratory syndrome coronavirus induces protective neutralizing antibodies primarily targeting the receptor binding region., Journal of Virology. 2005, 79(5): 2678-88.

Cheng V.C.C., Tang S.F., Wu A.K., Chu C.M. and Yuen K.Y., Medical treatment of viral pneumonia including SARS in immunocompetent adult., The Journal of infection. 2004, 49(4): 262-73.

Cheung C.Y., Poon L.L.M., Ng I.H.Y., Luk W., Sia S.F., Wu H.S., Chan K.H., Yuen K.Y., Gordon S., Guan Y. and Peiris J.S.M., Cytokine responses in severe acute respiratory syndrome coronavirus-infected macrophages in vitro: possible relevance to pathogenesis., Journal of Virology. 2005, 79(12): 7819-26.

Chu C.M., Leung W.S., Cheng V.C.C., Chan K.H., Lin A.W., Chan V.L., Lam J.Y., Chan K.S. and Yuen K.Y., Duration of RT-PCR positivity in severe acute respiratory syndrome., The European respiratory journal. 2005, 25(1): 12-4.

Chu C.M., Poon L.L.M., Cheng V.C.C., Chan K.S., Hung I.F., Wong M.M., Chan K.H., Leung W.S., Tang S.F., Chan V.L., Ng W.L., Sim T.C., Ng P.W., Law K.I., Tse D.M., Peiris J.S.M. and Yuen K.Y., Initial viral load and the outcomes of SARS., Canadian Medical Association journal . 2004, 171(11): 1349-52.

He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Ho P.L., Ho A.Y., Chow K.H., Wong R.C., Duan R.S., Ho W.L., Mak G.C., Tsang K.W., Yam W.C. and Yuen K.Y., Occurrence and molecular analysis of extended-spectrum {beta}-lactamase-producing Proteus mirabilis in Hong Kong, 1999-2002., The Journal of antimicrobial chemotherapy. 2005, 55(6): 840-5.

Hung I.F., Cheng V.C.C., Wu A.K., Tang S.F., Chan K.H., Chu C.M., Wong M.M., Hui W.T., Poon L.L.M., Tse D.M., Chan K.S., Woo P.C.Y., Lau S.K.P., Peiris J.S.M. and Yuen K.Y., Viral loads in clinical specimens and SARS manifestations., Emerging infectious diseases. 2004, 10(9): 1550-7.

Kao R.Y.T., To A.P.C., Ng L.W.Y., Tsui W.H.W., Lee T.S.W., Tsoi H.W. and Yuen K.Y., Characterization of SARS-CoV main protease and identification of biologically active small molecule inhibitors using a continuous fluorescence-based assay., FEBS letters. 2004, 576(3): 325-30.

Lau S.K.P., Woo P.C.Y., Fung A.M., Chan K.M., Woo G.K.S. and Yuen K.Y., Anaerobic, non-sporulating, Gram-positive bacilli bacteraemia characterized by 16S rRNA gene sequencing., Journal of medical microbiology. 2004, 53(Pt 12): 1247-53.

Lau S.K.P., Ho P.L., Li M.W., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and characterization of a chromosomal class C beta-lactamase and its regulatory gene in Laribacter hongkongensis., Antimicrobial agents and chemotherapy. 2005, 49(5): 1957-64.

Lau S.K.P., Woo P.C.Y., Wong B.H.L., Tsoi H.W., Woo G.K.S., Poon R.W.S., Chan K.H., Wei W.I., Peiris J.S.M. and Yuen K.Y., Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay., Journal of clinical microbiology. 2004, 42(7): 2884-9.

Lau S.K.P., Woo P.C.Y., To P.C.A., Lau T.Y. and Yuen K.Y., Lack of evidence that DNA in antibiotic preparations is a source of antibiotic resistance genes in bacteria from animal or human sources., Antimicrob Agents Chemother. 2004, 48(8): 3141-6.

Lau S.K.P., Woo P.C.Y., Chan C.Y., Wu W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi., Journal of clinical microbiology. 2005, 43(3): 1470-2.

Leung A.Y.H., Chan M.T.L., Yuen K.Y., Cheng V.C.C., Chan K.H., Wong L.P., Liang R.H.S., Lie A.K.W. and Kwong Y.L., Ciprofloxacin Decreased Polyoma BK Virus Load in Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation, Clinical Infectious Disease. 2005, 40: 528-537.

Leung A.Y.H., Chan M.T.L., Cheng V.C.C., Lie A.K.W., Yuen K.Y. and Kwong Y.L., Polyoma BK viruria in patients undergoing autologous hematopoietic stem cell transplantation, Bone Marrow Transplantation. 2005, 35: 1029−1030.

Leung A.Y.H., Chan M.T.L., Cheng V.C.C., Yuen K.Y. and Kwong Y.L., Quantification of Adenovirus in the Lower Respiratory Tract of Patients without Clinical Adenovirus-Related Respiratory Disease, Clinical Infectious Disease. 2005, 40: 1541-1544(online).

Li K.S., Guan Y., Wang J., Smith G.J., Xu K.M., Duan L., Rahardjo A.P., Puthavathana P., Buranathai C., Nguyen T.D., Estoepangestie A.T., Chaisingh A., Auewarakul P., Long H.T., Hanh N.T., Webby R.J., Poon L.L.M., Chen H., Shortridge K.F., Yuen K.Y., Webster R.G. and Peiris J.S.M., Genesis of a highly pathogenic and potentially pandemic H5N1 influenza virus in eastern Asia., Nature. 2004, 430(6996): 209-13.

Nal-Rogier B.T.M., Chan C.M., Kien F.S., Siu Y.L., Tse K.S., Chu S.L., Kam Y.W., Staropoli I., Crescenzo-Chaigne B., Escriou N., van der Werf S., Yuen K.Y. and Altmeyer R.M., Differential maturation and subcellular localization of severe acute respiratory syndrome coronavirus surface proteins S, M and E, Journal Of General Virology. Great Britain, 2005, 86: 1423-1434.

Ng K.H., Wu A.K., Cheng V.C.C., Tang S.F., Chan C.Y., Yung C.Y., Luk S.H., Lee T.W., Chow L. and Yuen K.Y., Pulmonary artery thrombosis in a patient with severe acute respiratory syndrome., Postgraduate medical journal. 2005, 81(956): e3.

Peiris J.S.M., Guan Y. and Yuen K.Y., Severe acute respiratory syndrome., Nature medicine. 2004, 10(12 Suppl): S88-97.

Peiris J.S.M., Guan Y. and Yuen K.Y., Special Research Achievement Award for the Research Team in the Department of Microbiology 2004., The University of Hong Kong. 2004.

Poon L.L.M., Wong B.W., Chan K.H., Leung C.S., Yuen K.Y., Guan Y. and Peiris J.S.M., A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors., Journal of clinical virology. 2004, 30(3): 214-7.

Poon L.L.M., Leung C.S.W., Chan K.H., Lee H.C., Yuen K.Y., Guan Y. and Peiris J.S.M., Detection of human influenza A viruses by loop-mediated isothermal amplification., Journal of Clinical Microbiology. 2005, 43(1): 427-30.

Poon L.L.M., Chu K.W., Chan K.H., Wong O.K., Ellis T.M., Leung C.Y.H., Lau S.K.P., Woo P.C.Y., Suen K.Y., Yuen K.Y., Guan Y. and Peiris J.S.M., Identification of a novel coronavirus in bats., Journal of Virology. 2005, 79(4): 2001-9.

Poon L.L.M., Guan Y., Nicholls J.M., Yuen K.Y. and Peiris J.S.M., The aetiology, origins, and diagnosis of severe acute respiratory syndrome., Lancet Infectious Diseases. 2004, 4(11): 663-71.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Eco-epidemiology of Laribacter Hongkongensis, a novel bacterium associated with gastroenteritis, 9th Research Postgraduate Symposium., 2004.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H., Ng L.T., Hui W.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis., Journal of clinical microbiology. 2005, 43(2): 919-22.

Wong S.S.Y. and Yuen K.Y., Human infection by avian influenza A H5N1., Hong Kong Medical Journal. Hong Kong, Hong Kong Academy of Medicine, 2005, 11: 189-199.

Wong S.S.Y. and Yuen K.Y., Penicilliosis in China, Mycopathologia. 2004, 158: 147-150.

Wong S.S.Y. and Yuen K.Y., Red imported fire ants in Hong Kong, Hong Kong Medical Journal. Hong Kong, Hong Kong Academy of Medicine, 2005, 11: 131-132.

Wong S.S.Y., Woo P.C.Y. and Yuen K.Y., Unusual laboratory findings in a case of Norwegian scabies provided a clue to diagnosis, Journal of Clinical Microbiology. 2005, 43: 2542-2544.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia., Journal of virology. 2005, 79(2): 884-95.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess., Systematic and applied microbiology. 2005, 28(4): 316-22.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous drug abuser with pseudobacteremia., Microbiology and immunology. 2005, 49(1): 31-9.

Woo P.C.Y., Lau S.K.P., Woo G.K.S., Fung A.M., Yiu V.P. and Yuen K.Y., Bacteremia due to Clostridium hathewayi in a patient with acute appendicitis., Journal of clinical microbiology. 2004, 42(12): 5947-9.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Wong M.K.M. and Yuen K.Y., Bacteremia in a patient with colonic carcinoma caused by a novel Sedimentibacter species: Sedimentibacter hongkongensis sp. nov., Diagnostic microbiology and infectious disease. 2004, 50(2): 81-7.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Biliary tract disease as a risk factor for Plesiomonas shigelloides bacteraemia: a nine-year experience in a Hong Kong hospital and review of the literature., The new microbiologica. 2005, 28(1): 45-55.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M., Tang S.F. and Yuen K.Y., Clostridium bacteraemia characterised by 16S ribosomal RNA gene sequencing., Journal of clinical pathology. 2005, 58(3): 301-7.

Woo P.C.Y., Wong S.S.Y., Lau S.K.P. and Yuen K.Y., Continuous ambulatory peritoneal dialysis-related peritonitis associated with Lancefield group G beta-hemolytic streptococcus: report of two cases requiring Tenckhoff catheter removal., Journal of clinical microbiology. 2004, 42(9): 4399-402.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Chan K.H., Hui W.T., Kwan G.S., Peiris J.S.M., Couch R.B. and Yuen K.Y., False-positive results in a recombinant severe acute respiratory syndrome-associated coronavirus (SARS-CoV) nucleocapsid enzyme-linked immunosorbent assay due to HCoV-OC43 and HCoV-229E rectified by Western blotting with recombinant SARS-CoV spike polypeptide., Journal of clinical microbiology. 2004, 42(12): 5885-8.

Woo P.C.Y., Lau S.K.P., Wong B.H., Chan K.H., Chu C.M., Tsoi H.W., Huang Y., Peiris J.S.M. and Yuen K.Y., Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus, Clinical and diagnostic laboratory immunology. 2004, 11(4): 665-8.

Woo P.C.Y., Lau S.K.P., Lau C.C., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient., Bone marrow transplantation. 2005, 35(8): 831-3.

Woo P.C.Y., To P.C.A., Lau S.K.P., Fung A.M. and Yuen K.Y., Phenotypic and molecular characterization of erythromycin resistance in four isolates of Streptococcus-like gram-positive cocci causing bacteremia., Journal of clinical microbiology. 2004, 2004: 3303-5.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Tse H., Wong B.H.L. and Yuen K.Y., Streptococcus sinensis may react with Lancefield group F antiserum., Journal of medical microbiology. 2004, 53(Pt 11): 1083-8.

Wu A.K., Cheng V.C.C., Tang S.F., Hung I.F., Lee R.A., Hui D.S. and Yuen K.Y., The unmasking of Pneumocystis jiroveci pneumonia during reversal of immunosuppression: case reports and literature review., BMC infectious diseases. 2004, 4(1): 57.

Xia X. and Yuen K.Y., Differential selection and mutation between dsDNA and ssDNA phages shape the evolution of their genomic AT percentage., BMC genetics. 2005, 6(1): 20.

Yam W.C., Chan K.H., Chow K.H., Poon L.L.M., Lam H.Y., Yuen K.Y., Seto W.H. and Peiris J.S.M., Clinical evaluation of real-time PCR assays for rapid diagnosis of SARS coronavirus during outbreak and post-epidemic periods., J Clin Virol. USA, 2005, 33(1): 19-24.

Yam W.C., Tam C.M., Leung C.C., Chan K.H., Leung T.Y., Wong K.C., Yew W.W., Seto W.H., Yuen K.Y. and Ho P.L., Direct Detection of Rifampin-Resistant Mycobacterium tuberculosis in Respiratory Specimens by PCR-DNA sequencing., J Clin Microbiol . USA, ASM, 2004, 42: 4438-4443.

Yuen K.Y., Award of the Silver Bauhinia Star 2004, Hong Kong Government, 2004.

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zhao X.Y., Lee S.S., Wong K.H., Chan K.C., Ma S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Effects of single nucleotide polymorphisms in the RANTES promoter region in healthy and HIV-infected indigenous Chinese., European journal of immunogenetics. 2004, 31(4): 179-83.

Zheng B., Guan Y. and Yuen K.Y., SARS – from animals to man: New Viral Threats to Human Health Worldwide, 12° CONGRESSO NAZIONALEdella SOCIETÀ di MICROBIOLOGIA MEDICA ODONTOIATRICA e CLINICA. 20-22 July, 2004. Genova, Italy.. 2004.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Yung RWH

List of Research Outputs

Lau S.K.P., Ho P.L., Li M.W., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and characterization of a chromosomal class C beta-lactamase and its regulatory gene in Laribacter hongkongensis., Antimicrobial agents and chemotherapy. 2005, 49(5): 1957-64.

Researcher : Zhang C

List of Research Outputs

Li W.S., Zhang C., Sui J., Kuhn H., Moore M.J., Luo S., Wong S.K., Huang I.C., Xu K., Vasilieva N., Murakami A., He Y., Marasco W.A., Guan Y., Choe H. and Farzan M., Receptor and viral determinants of SARS-coronavirus adaptation to human ACE2. , The EMBO journal. 2005, 24(8): 1634-1643.

Researcher : Zhang Z

List of Research Outputs

Yuen R.M.F., Wong D.K.H., Chan C.S., Zhang Z., Yuen J.C.H. and Lai C.L., Immune Response During Immunoclearance Phase of Chinese Patients with HBV Genotypes B and C, Journal of Hepatology. 2005, 42 (Suppl 2): 152.

Researcher : Zhao X

List of Research Outputs

Duan Z.P., Zhao X., Huang D.Z., He L.X., Yu C.Y., Zhao C.H. and Zheng B., RANTES gene single nucleotide polymorphisms and expression in chronic Hepatitis B Virus infection., Chinese Medical Journal (Engl). 2005, 118: 909-914.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zheng B., Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ma S. and Ng M.H., Effects of single nucleotide polymorphism in the RANTES promoter region on HIV infection and disease progression in indigenous Chinese, XV international AIDS conference. 11-16 July, 2004. Bangkok, Thailand.. 2004.

Researcher : Zheng B

Project Title:	The pattern of chemokine receptor polymorphism in healthy and HIV positive Chinese adults
Investigator(s):	Zheng B, Lee SS
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	01/2001

Abstract:

To determine the prevalence of the major forms of CCR5 promoter and CCR2 polymorphism in healthy Chinese adults; to compare the pattern between healthy adults and HIV infected individuals in Hong Kong.

Project Title:	CCR5 expression on antigen-presenting cens in HIV infection
Investigator(s):	Zheng B, Lee SS
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	01/2001

Abstract:

To describe the pattern of CCR5 expression on APC in HIV infection; to correlate this pattern to HIV infected APC; to determine the functional and/or quantitative deficiency in CCR5 expressing APC.

Project Title:	Functional studies of dendritic cells in chronic Chinese Hepatitis B patients
Investigator(s):	Zheng B, Yuen KY, Qu D, Zhang J.M., Wen YM, Zhu N.S.
Department:	Microbiology
Source(s) of Funding:	NSFC/RGC Joint Research Scheme
Start Date:	12/2001

Abstract:

To ascertain if defective DC indeed contribute to CHBVI and if the defect may account for the immune tolerance; to investigate the mechanism of the DC functional deficit; to determine the extent to which it contributes to the infection; to investitate whether the defect may affectimmune response to other viruses or the general immune status of the individuals; to determine whether the defect may be overcome by ex vivo cytokine treatment to restore the function of DC and/or by improving antigen uptake by DC.

Project Title:	Primary study for development of an inactivated coronavirus vaccine
Investigator(s):	Zheng B
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To develop a coronavirus inactivated vaccine (CI-1) for the prevention of SARS-associated coronavirus infection.

Project Title:	A study of dendritic cell-based vaccine for immune therapy of chronic hepatitis B virus infection
Investigator(s):	Zheng B, Lu L, Ng MH
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To investigate the hypothesis that DC-based vaccine (cytokine-activated monocyte-derived DCs used as vectors to deliver HBsAg or HBcAg) may be crucial in immunotherapy of chronic HBV infection.

Project Title:	A study of antiviral peptides targeting spike and matrix proteins of SARS coronavirus
Investigator(s):	Zheng B, Guan Y
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (Special Grants for Severe Acute Respiratory Syndrome Research)
Start Date:	09/2003
Completion Date:	08/2004

Abstract:

To test the hypothesis that some of amino acid residues in spike (S) and matrix (M) proteins that are different between human SCoV and animal SCoV-like viruses might determine whether the virus infects human or animals and that peptides designed based on these substitutions might interfere with the formation of fusion-active structures and therefore block the entry of the virus into host cells.

Project Title:	Studies of SARS virus vaccines
Investigator(s):	Zheng B, Guan Y
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2004

Abstract:

To develop SCoV vaccines for prevention of SARS; to develop an inactivated vaccine and a cold attenuated live vaccine.

Project Title:	A study of SARS virus animal vaccines
Investigator(s):	Zheng B
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

List of Research Outputs

Bernini A., Spiga O., Ciutti A., Chiellini S., Bracci L., Yan X.Y., Zheng B., Huang J., He M.L., Song H.D., Hao P., Zhao G.P. and Niccolai N., Prediction of quaternary assembly of SARS coronavirus peplomer, Biochemical and Biophysical Research Communications. 2004, 325: 1210-1214.

Cao K., Zheng Y., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H. and Zheng B., Peripheral NK Cells Play a Complementary Role for Anti-tumor Immunity in Nasopharyngeal Carcinoma Patients., Keystone Symposia – Basic Aspects of Tumor Immunology II (C3). Keystone, Colorado, USA.. 2005.

Chan C.P., Siu K.L., Zheng B. and Jin D., Subcellular localization of sars coronavirus orf8 protein, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Di B., Hao W., Gao Y., Wang M., Wang Y., Qiu L.W., Wen K., Zhou D.H., Wu X.W., Lu E.J., Liao Z.Y., Mei Y.B., Zheng B. and Che X.Y., Monoclonal Antibody-based Antigen Capture Enzyme-linked Immunosorbent Assay Reveals High Sensitivity Of The Nucleocapsid Protein In Acute-phase Sera Of Severe Acute Respiratory Syndrome Patients., Clinical and Diagnostic Laboratory Immunology. 2005, 12: 135-140.

Duan Z.P., Zhao X., Huang D.Z., He L.X., Yu C.Y., Zhao C.H. and Zheng B., RANTES gene single nucleotide polymorphisms and expression in chronic Hepatitis B Virus infection., Chinese Medical Journal (Engl). 2005, 118: 909-914.

He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Qu D., Zheng B., Yao X., Guan Y., Yuan Z.H., Zhong N.S., Lu L., Xie J. and Wen Y., Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) in mice induced local and serum antibodies., Vaccine. 2005, 23: 924-931.

Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.

Wang M., Xu H.F., Mo Z.Y., Zheng B., Gu J., Qin P.Z., Zhang Z.B., Zou X.Y., Liang C.Y., Zhao Y.T. and Gao K., Anti-SARS virus antibody responses against human SARS-associated coronavirus and animal SARS-associated coronavirus-like virus, Chin Med J (Eng). 2004, 117: 1723-1725.

Wang M., Gao Y., Zhou D.H., Wu X.W., Chen X.S., Di B., Liu Y.F., Chen F., Du L., Xu H.F., Gu J., Zheng B. and Xu J.G., The effects of 4 laboratory test kits in early detecting of severe acute respiratory syndrome coronavirus., Zhonghua Liu Xing Bing Xue Za Zhi. 2005, 26: 22-24.

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., He Z., Ma S., Ng F., Chan C.S., Ho T., Ng M.H. and Zheng B., A novel CCR5 mutation selectively affects immunoreactivity and fusogenic property of the HIV co-receptor in a slow progressing HIV patient., AIDS. 2004, 18: 1729-1732.

Zhao X., Lee S.S., Wong K.H., Chan K.C., Ng F., Chan C.C., Han D., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Functional analysis of naturally occurring mutations in the open reading frame of CCR5 in HIV-infected Chinese patients and healthy controls., Journal of acquired immune deficiency syndromes. 2005, 38(5): 509-17.

Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ng F., Chan C.S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Polymorphisms and Functional Analysis of CCR5 Gene in Southern Chinese HIV Patients and Healthy Controls., Keystone Symposia – HIV Pathogenesis (X7). 9-15 April, 2005. Banff, Alberta, Canada.. Banff, Alberta, Canada., 2005.

Zhao X.Y., Lee S.S., Wong K.H., Chan K.C., Ma S., Yam W.C., Yuen K.Y., Ng M.H. and Zheng B., Effects of single nucleotide polymorphisms in the RANTES promoter region in healthy and HIV-infected indigenous Chinese., European journal of immunogenetics. 2004, 31(4): 179-83.

Zheng B., Animal source of SARS, The 4th national conference for Microbiology and Immunology. 14-16 September, 2004. Chongqing, China.. 2004.

Zheng B., Zhao X., Lee S.S., Wong K.H., Chan K.C.W., Ma S. and Ng M.H., Effects of single nucleotide polymorphism in the RANTES promoter region on HIV infection and disease progression in indigenous Chinese, XV international AIDS conference. 11-16 July, 2004. Bangkok, Thailand.. 2004.

Zheng B., He M.L., Wong K.L., Lum C.T., Poon L.L.M., Peng Y., Guan Y., Lin M.C. and Kung H., Potent inhibition of SARS-associated coronavirus (SCoV) infection and replication by type I interferons (IFN-alpha/beta)but not by type II interferon (IFN-gamma), Journal of Interferon and Cytokine Research. 2004, 24(7): 388-90.

Zheng B., Guan Y. and Yuen K.Y., SARS – from animals to man: New Viral Threats to Human Health Worldwide, 12° CONGRESSO NAZIONALEdella SOCIETÀ di MICROBIOLOGIA MEDICA ODONTOIATRICA e CLINICA. 20-22 July, 2004. Genova, Italy.. 2004.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Zheng Y

List of Research Outputs

Cao K., Zheng Y., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H. and Zheng B., Peripheral NK Cells Play a Complementary Role for Anti-tumor Immunity in Nasopharyngeal Carcinoma Patients., Keystone Symposia – Basic Aspects of Tumor Immunology II (C3). Keystone, Colorado, USA.. 2005.

Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.

Researcher : Zhou J

List of Research Outputs

Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.

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