Dept of Biochemistry

Research and Scholarship 2005

DEPARTMENT OF BIOCHEMISTRY



Researcher : Chan CP

List of Research Outputs

Chan C.P., Siu K.L., Zheng B. and Jin D., Subcellular localization of sars coronavirus orf8 protein, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Chin K.T., Zhou H., Wong C.M., Lee M.F., Chan C.P., Qiang B.Q., Yuan J.G., Ng I.O.L. and Jin D., The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma, Nucleic Acids Research. 2005, 33(6): 1859-1873.


Researcher : Chan D

Project Title:Molecular and cellular changes underlying increased bone formation in a mouse model with generalized progressive hyperostosis
Investigator(s):Chan D, Cheah KSE
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:10/2001
Abstract:
To determine the fate of Co110-13del protein by analysing its synthesis, secretion and degradation in primary osteoblast cultures, and correlate the finding with possible contributing factors; to test primary osteoblasts for constitutive activation and the capacity to promote active bone formation by monitoring collagen synthesis, in vitro matrix formation/composition, mineralisation, and matrix architecture surrounding cells; to determine whether osteoclast activity and/or osteoclastogenesis contribute to the increase-bone phenotype.


Project Title:A matrix metalloproteinase detector for in vitro monitoring of enzyme activity
Investigator(s):Chan D, Lam E
Department:Biochemistry
Source(s) of Funding:Research Initiation Programme
Start Date:06/2002
Abstract:
To establish efficient in vitro reporter assays for aggrecanase and other MMPs.


Project Title:The role of matrix remodeling in endochondral bone formation
Investigator(s):Chan D, Cheah KSE
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:10/2002
Abstract:
The project attempts to:- 1) create heterozygous and homzygous mouse mutants expressing MMP-resistant collagen X from the four embryonic stem (ES) cell clones; 2) perform detailed in virto and in vivo analysis to study the molecular and phenotypic consequences on endochondral bone formation.


Project Title:Understanding the molecular basis of skeletal (digit) patterning defects in Brachydactylyl type A-1
Investigator(s):Chan D, Cheah KSE, He L.
Department:Biochemistry
Source(s) of Funding:NSFC/RGC Joint Research Scheme
Start Date:01/2003
Abstract:
To create a BDA-1 mouse model by gene targeting; to study the impact of the BDA-1 mutation on skeletal morphogenesis; to study the biochemical consequence of BDA-1 mutations using in vitro cell approaches.


Project Title:Defining the molecular basis and 'factors' promoting osteoblast activity in a mouse model with generalized hyperostosis
Investigator(s):Chan D, Cheah KSE, Cheung KMC, Chu IK
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2003
Abstract:
To define the molecular basis and "factors" promoting osteoblast activity in a mouse model with generalized progressive hyperostosis.


Project Title:The biology of digit formation and its pathology in brachydactyly
Investigator(s):Chan D, Mundlos S.
Department:Biochemistry
Source(s) of Funding:Germany/Hong Kong Joint Research Scheme
Start Date:01/2005
Abstract:
To characterize the Ihh-E95K mouse phenotype focusing on endochondral ossification and digit formation; to investigate interacting brachydactyly pathways using a genetic approach in mice; to create Hoxd13 targeting constructs with expanded poly-Als repeats and perform gene-targeting in ES cells.


Project Title:Genomic analysis of the regulation of osteoblast activity in a mouse with generalized hyperostosis
Investigator(s):Chan D, Cheah KSE, Cheung KMC
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2005
Abstract:
To analyses of specific markers for known regulators of osteoblast differentiation, proliferation and biosynthetic activity; to use gene expression profiling using micro-arrays to gain insights into genes that are expressed in normal and 13del-tg osteoblasts to identify alterations in pathways as a consequence of expressing Col10-13del; to create a transgenic mouse expression of Col10-13del in osteoblasts to assess its expression and correlation to the hyperostosis phenotype.


Project Title:Genomic analysis of the regulation of osteoblast activity in a mouse with generalized hyperostosis
Investigator(s):Chan D
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
Refer to hard copy


List of Research Outputs

Abbah S.A., Lu W.W., Chan D., Liu W.G., Li Z.Y. and Luk K.D.K., New bone formation with osteoprogenitor cells confined in sodium alginate microcapsules , 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Chan D., Yang L., Hui W.S., Chan W.C.W., Huang J., Shum D.K.Y., Cheung K.M.C., Luo Z.J. and Cheah K.S.E., A splicing mutation in the ext2 gene affects messenger RNA stability in a family with hereditary multiple exostoses, XIXth Meeting of the Federation of the European Connective Tissue Societies, Taormina-Giardini Noxos, Italy. 9-13 July 2004.
Chan W.Y., Cheah K.S.E., Ng V.C.W., Murphy G. and Chan D., Mouse model with impaired collagen X degradation at the chondro-osseous junction, Gordon Research Conference: Biology and Pathology of Cartilage. Il Ciocco, Barga, Italy June 2005.
Chan W.Y. and Chan D., Mouse model with impaired matrix remodeling in endochondral ossification, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., So C.L., Kwan K.M., Hunziker E.B., Yamada Y., Bateman J.F., Cheung K.M.C. and Chan D., Chondrocytes survive ER stress caused by unfolded proteins via re-programming, HGM2005 HUGO's 10th Human Genome Meeting, Kyoto, Japan 18-21 April 2005.
Cheah K.S.E., Niewiadomska A.K., Dung W.F., Lau J.Y.B., Hunziker E., Aszodi A., Gustafsson E., Yamada Y., Zhou Z., Tryggvason K. and Chan D., Perlecan compartmentalizes collagen X in the mammalian growth plate., XIXth meeting of Federation of the European Connective Tissue Societies: Taormina-Giardia Naxos, Italy July 2004.
Cheng Y.W., Chan W.C.W., Ng V.C.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse expressing mutant type X collagen , 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Cheng Y.W., Chan W.C.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse expressing mutant type X collagen, 51st Annual Meeting of the Orthopaedic Research Society, Washington DC, USA, February 20-23, 2005.
Cheng Y.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse model expressing mutant type X, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Cheung K.M.C., Ho G. and Chan D., Intervertebral disc regeneration by use of autologous mesenchymal stem cells, an experimental model in rabbits, 39th Annual Meeting of the Scoliosis Research Society, Buenos Aires, Argentina, September 6-9, 2004.
Gao B., Cheah K.S.E., He L. and Chan D., Delayed endochondral ossification and digit joint formation in mice expressing a brachydactyly type A1 mutation in Indian Hedgehog, HGM2005 Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005 . 2005.
Guo X., Irwin M.G., Chan D., Tipoe G.L. and Cheung K.M.C., Differential fuctions of cyclooxygenase 1 and 2 in bone repair, European Cells and Materials. 2005, 10 Suppl. 3: 56.
Guo X., Irwin M.G., Cheung K.M.C. and Chan D., The effects of cyclooxygenase -2 selective non-steroidal anti-inflammatory drugs (NSAIDs) in fracture repair , 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Guo X., Cheung K.M.C., Chan D. and Irwin M.G., The effects of cyclooxygenase-2 selective non-steroidal anti-inflammatory drugs (NSAIDs) in fracture repair, 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Ho G., Chan D. and Cheung K.M.C., Intervertebral disc regeneration by use of autologous mesenchymal stem cells, an experimental model in rabbits, 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Jim J.J.T., Chan D., Cheah K.S.E., Luk K.D.K. and Cheung K.M.C., Isolation and detection of type IX collagen from adult human intervertebral disc, 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Sham P.C., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., The TRP2 allele of collagen IX as an age-dependent risk factor for the development and severity of intervertebral disc degeneration, Asia Pacific Orthopaedic Association 14th Triennial Congress, 5-10 September 2004.
Poon R.W.Y., Yeung K.W.K., Liu X.Y., Chu P.K., Chung C.Y., Lu W.W., Cheung K.M.C. and Chan D., Carbon plasma immersion ion implantation of Nickel-Titanium shape memory alloys, Biomaterials. 2005, 26(15): 2265-2272.
Song Y., Cheung K.M.C., Karppinen J., Ho D.W.H., Yip S...P., Leong J...C...Y., Ott J., Luk K.D.K., Cheah K.S.E., Sham P.C. and Chan D., Association studies of intervertebral disc disease with genes in the aggrecan degradation pathway, HUGO 2005: Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005.
Wilson R., Freddi S., Chan D., Cheah K.S.E. and Bateman J.F., Misfolding of collagen X chains harboring schmid metaphyseal chondrodysplasia mutations results in aberrant disulfide bond formation, intracellular retention, and activation of the unfolded protein response., Journal of Biological Chemistry. 2005, 22;280(16): 15544-52.
Wong C.T., Lu W.W., Chan D., Cheung K.M.C. and Luk K.D.K., Stimulation of in vitro mineralization on strontium-containing hydroxyapatite bioactive bone cement, 51st Annual Meeting of the Orthopaedic Research Society, Washington DC, USA, February 20-23, 2005.
Wong Q.N.Y., Ng V.C.W., Lin M.C., Huang F.Y., Chan D. and Huang J., Efficient and seamless DNA recombineering using a thymidylate synthase a selection system in Escherichia coli, Nucleic Acids Research. 2005, 33(6): 2-9.
Yang L., Chan D. and Cheah K.S.E., Genetic analyses of terminal differentiation of hypertrophic chondrocytes, 9th Research Postgraduate Symposium, HKU, December 4, 2004.
Yeung K.W.K., Poon R.W.Y., Liu X.Y., Ho J.P.Y., Chung C.Y., Chu P.K., Chan D., Lu W.W. and Cheung K.M.C., An in-vitro and surface chemical analysis of nickel-titanium alloys enhanced by plasma ion implantations surface treatment, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Yeung K.W.K., Poon R.W.Y., Liu X.Y., Ho J.P.Y., Chung C.Y., Chu P.K., Lu W.W., Chan D. and Cheung K.M.C., Enhanced corrosion resistance and biocompatibility by plasma implantation of nickel titanium alloys: an in vitro and surface chemical study, 51st Annual Meeting of the Orthopaedic Research Society, Washington DC, USA, February 20-23, 2005.
Yeung K.W.K., Poon R.W.Y., Liu X.Y., Ho J.P.Y., Chung C.Y., Chu P.K., Lu W.W., Chan D. and Cheung K.M.C., Investigation of nickel suppression and cyto-compatibility of surface treated NiTi shape memory alloy by using plasma immersion ion implantation, Journal of Biomedical Materials Research: Part A. 2005, 72A(3): 238-245.
Yeung K.W.K., Poon R.W.Y., Liu X.Y., Ho J.P.Y., Chung C.Y., Chu P.K., Chan D., Lu W.W. and Cheung K.M.C., Study of enhanced corrosion resistance and biocompatibility by plasma implantation of nickel-titanium alloys: an in-vitro and surface chemcial analysis, 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Yeung K.W.K., Poon R.W.Y., Liu X.Y., Ho J.P.Y., Chu P.K., Chung C.Y., Lu W.W., Chan D. and Cheung K.M.C., Study of nickel suppression and cyto-compatibility of plasma immersion ion implantation treated nickel titanium shape memory alloys, BME 2004 Conference, Hong Kong, September 23-25, 2004 . 2004.


Researcher : Chan JKM

List of Research Outputs

Chan J.K.M. and Zhou Z., MIT-MMP in FGF signaling, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Chan J.K.M. and Zhou Z., MT1-MMP in FGF Signaling, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Liu B., Chan J.K.M., Huang J. and Zhou Z., Premature aging and genomic instability in mice lacking zmpste24, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Zhou Z., Wang J., Cao R., Morita H., Soininen R., Chan J.K.M., Liu B., Cao Y. and Tryggvason K., Impaired angiogenesis, delayed wound healing and retarded tumor growth in perlecan heparan sulfate-deficient mice, Cancer Research. 2004, 15;64(14): 4699-702.


Researcher : Chan KFJ

Project Title:Signal transduction in the brain: roles of ganglioside-stimulated protein kinase
Investigator(s):Chan KFJ
Department:Biochemistry
Source(s) of Funding:University Research Committee / Committee on Research and Conference Grants - General Award
Start Date:07/1993
Abstract:
To establish the functional roles for a novel ganglioside-stimulated protein kinase. These investigations are designed to provide further evidence for our unifying hypothesis that gangliosides can serve as multifuncitonal biomodulators in neural signal transduction.





Researcher : Chan KK

List of Research Outputs

Chan K.K., Chen Y.S., Yau T.O., Fu M., Lui V.C.H., Tam P.K.H. and Sham M.H., Hoxb3 vagal neural crest-specific enhancer element for controlling enteric nervous system development, Developmental Dynamics. 2005, 233: 473-483.
Law M.L., Tsang W.H., Chan K.K. and Sham M.H., Abnormal enteric ganglia development in a SOX 10 mouse mutant generated by gene targeting, 9th Rearch Postgraduate Symposium, HKU4 December 2004.
Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interaction of Hoxb3 and Hoxb1 in hindbrain patterning revealed by ectopic expression of Hoxb3 in rhombomere 4 , 17 Annual Meeting on Mouse Molecular Genetics, Cold Spring Harbor Laboratory, New York, 1-5 September 2004.


Researcher : Chan KT

List of Research Outputs

Chan K.T. and Sham M.H., Disruption of the mouse Hoxb3 locus affects ventral body wall development, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Chan LC

List of Research Outputs

Au W.Y., Ma E.S.K., Lam V.M.S., Chan L.C., Pang A.W.K. and Kwong Y.L., Glucose 6-phosphate dehydrogenase (G6PD) deficiency in elderly Chinese women heterozygous for G6PD variants, American Journal of Medical Genetics Part A. 2004, 129A(2): 208 - 211.


Researcher : Chan SF

List of Research Outputs

Ng D.C.H., Ching Y.P., Chan S.F. and Jin D., Human T-cell leukemia virus type I oncoprotein tax targets the centrosome, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Chan TF

List of Research Outputs

Chan T.F., An analysis of two naturally-occurring G6PD deficient mutants, G6PD campinus AND G6PD fukaya, MPhil Thesis. 2005.


Researcher : Chan WCW

List of Research Outputs

Chan D., Yang L., Hui W.S., Chan W.C.W., Huang J., Shum D.K.Y., Cheung K.M.C., Luo Z.J. and Cheah K.S.E., A splicing mutation in the ext2 gene affects messenger RNA stability in a family with hereditary multiple exostoses, XIXth Meeting of the Federation of the European Connective Tissue Societies, Taormina-Giardini Noxos, Italy. 9-13 July 2004.
Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., So C.L., Kwan K.M., Hunziker E.B., Yamada Y., Bateman J.F., Cheung K.M.C. and Chan D., Chondrocytes survive ER stress caused by unfolded proteins via re-programming, HGM2005 HUGO's 10th Human Genome Meeting, Kyoto, Japan 18-21 April 2005.
Cheng Y.W., Chan W.C.W., Ng V.C.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse expressing mutant type X collagen , 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Cheng Y.W., Chan W.C.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse expressing mutant type X collagen, 51st Annual Meeting of the Orthopaedic Research Society, Washington DC, USA, February 20-23, 2005.


Researcher : Chan WY

List of Research Outputs

Chan W.Y., Cheah K.S.E., Ng V.C.W., Murphy G. and Chan D., Mouse model with impaired collagen X degradation at the chondro-osseous junction, Gordon Research Conference: Biology and Pathology of Cartilage. Il Ciocco, Barga, Italy June 2005.
Chan W.Y. and Chan D., Mouse model with impaired matrix remodeling in endochondral ossification, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Cheah KSE

Project Title:The role of Type II procollagens encoded by alternatively spliced forms of aI(II) procollagen mRNA in cartilage differnetiation and growth
Investigator(s):Cheah KSE
Department:Biochemistry
Source(s) of Funding:Arthritis and Rheumatism Council (ARC) - General Award
Start Date:12/1993
Abstract:
To gain insight into the function of type IIA procollagen by: a) performing a loss-of function test by introducing into the mouse germ line, a mutation in the [alpha]1(II) collagen gene in which exon 2 is deleted, using gene targeting; determining the phenoypic consequence of reduced levels of expression or failure to express type IIA procollage; generating monoclonal antibodies to the cysteine-rich domain within the aminopropeptide of type IIA procollagen.


Project Title:Defining the role of the transcription factor Sox9 in skeletal development by tissue-specific gene inactivation
Investigator(s):Cheah KSE, Lovell-Badge R.H.
Department:Biochemistry
Source(s) of Funding:UK/Hong Kong Joint Research Scheme
Start Date:02/1998
Abstract:
To inactivate the mouse Sox9 gene selectively and specifically in developing cartilage tissue in order to understand the role of Sox9 in skeletal formation and the relationship between loss of Sox9 gene function and skeletal malformation.


Project Title:Molecular and transgenic approaches for the identification of genes regulated by SOX9 during mouse development
Investigator(s):Cheah KSE
Department:Biochemistry
Source(s) of Funding:Outstanding RGC Projects
Start Date:09/1998
Abstract:
To use a combination of molecular cloning, cell culture and transgenic/chimeric mouse approaches to: 1) identify other downstream targets of SOX9; 2) test the possibility that SOX9 may have a role as a negative regulator of transcription; 3) study SOX9 function by determining the developmental consequences of mis-expression of the gene in transgenic mice. These studies will provide fundatmental information on the mechanisms by which SOX9 regulates gene expression, with profound implications for understanding it's development role and the molecular basis of CD caused by loss of SOX9 function.


Project Title:Functional analysis of the morphogenetic role of type IIA procollagen during mouse development by conditional tissue-specific gene inactivation
Investigator(s):Cheah KSE, Chan D, Tam P.P.L.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2001
Abstract:
To use tissue-specific gene inactivation in mice, molecular embryology and biochemistry to test the hypothesis that IIA procollagen has a morphogenetic role in tissue patterning during organogenesis.


Project Title:In vivo functional analysis of the SOX9 transcription factor in regulating developmental gene expression
Investigator(s):Cheah KSE, Chan D, Tam P.P.L.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2001
Abstract:
To use transactivation of the COL2A1 enhancer reporter upon ectopic expression of SOX in transgenic mice to assess: i) the functional requirement for SOX9 to activate its target genes by testing the competence of SOX2 and SOX8 to substitute for SOX9; ii) the requirements for the N-, HMG, C terminus regions of SOX9 in activating its target genes by testing the competence of SOX9-SOX2 and SOX9-SOX8 chimeric proteins to substitute for SOX9 by gene targeting into the Sox9 locus in mouse ES cells, to test further the competence of the alternative SOX gene and/or relevant chimeric SOX protein to mediate the differentiation and developmental roles of SOX9, with particular focus on chondrogenesis.


Project Title:Genomic approaches to uncover functionally relevant signalling pathways in craniofacial development
Investigator(s):Cheah KSE, Wong BCW, Sham MH, Chung SK, Huang J, Smith DK, Wicking C.A., Yang MMS, Chow KL, Tam P.P.L.
Department:Biochemistry
Source(s) of Funding:Central Allocation Vote - Group Research Project
Start Date:06/2002
Abstract:
To build a multidisciplinary, competitive and productive research team of a critical size with the central theme of uncovering genetic relationships and functionally relevant signalling pathways in craniofacial development in order to make an impact in this important research area, currently at the fore-front of genomic biology; to pool expertise and resources, employing complementary genetic approaches in mice, coupled with advanced technology to reveal networks of genes and the complex interactions that specify morphology of the head skeleton and facial structures; to use and develop bioinformatics tools to analyse the expression data and formulate hypotheses on genetic relationships and the pathways regulating patterning, formation and growth of the craniofacial primordia; to test the hypotheses on potential functional relationships and molecular interactions revealed by the bioinformatic analyses, in the worm model and in the yeast two hybrid system.


Project Title:The regulation and role of Sox2 in the circling, deaf and yellow mouse mutants Yellow submarine (Ysb) and Light coat and circling (Lcc)
Investigator(s):Cheah KSE
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:10/2002
Abstract:
The current project will test some hypotheses of Sox2 function using gene mapping, comparative genomics, genetics and transgenesis to: - i) identify the chromosomal breakpoints in Lcc; ii) identify the mutations in Ysb and Lcc and determine whether these have disrupted cis-elements which regulate Sox2 expression in the inner ear and hair follicle; iii) validate these findings by functional rescue/mutagenesis/genetic experiments in transgenic mice; iv) identify the molecular developmental changes in inner ear hair cell and hair follicle morphogenesis.


Project Title:Understanding gene function and molecular bases of disease using transgenic and gene targeting technology
Investigator(s):Cheah KSE, Chan SY, Chan LC, Sham MH, Chung SK, Chow BKC, Kung H, Chan D, Huang J, Lin MC, Zhang JCL, Waye MMY, Lung M.L., Chow KL, Tam P.P.L., Chan W.Y., Lui VCH, Yao KM
Department:Biochemistry
Source(s) of Funding:Central Allocation Vote - Group Research Project
Start Date:06/2003
Abstract:
To continue to build up a multidisciplinary, competitive and productive research team of a critical size pooling expertise and resources to tackle several fore-front issues of human diseases using animal models; to maintain a highly productive Transgenic Core Facility (TCF) with extended "clientele", facilitating the timely generation of genetically modified mice for more projects which will serve to provide new knowledge in understanding gene function and as models of human disease, provide insight into the molecular pathogenesis of disease.


Project Title:Genetic manipulation and analyses of the regulation of chondrocyte hypertrophy
Investigator(s):Cheah KSE, Chan D
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:09/2003
Abstract:
To understand the roles of Ihh and Ppr specifically in hypertrophic chondrocytes (HCs) and will dissect the mode of action of the Ihh and PPR pathways in HCs, in vivo, in transgenic and conditional knockout mice, Specially we will: 1) Investigate whether abnormal Ihh signaling and /or processing in 13del HCs is a primary cause of the differentiation abnormality by a) ablating Smoothened (Smo), the transducer of Ihh signaling in 13 del and wild-type HCs and b) over-expressing either Ihh or a non-secreted and unprocessed form of Ihh in normal HCs. 2) By the same rationale, study the role of the PTHrP/PPR pathway by ablating PPR in 13 del and wild-type HCs.


Project Title:Functional analysis of SM22[beta] by tissue-specific targeted deletion in mice
Investigator(s):Cheah KSE, Lau CP, Feil R., Parmacek M.S., Zhang JCL
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2003
Abstract:
To determine the general role of SM22[beta] in a cell; to understand the specific role of SM22[beta] in cardiovascular development.


Project Title:Genetic manipulation and analyses of the regulation of chondrocyte hypertrophy
Investigator(s):Cheah KSE, Chan D
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2003
Abstract:
To understand the roles of Ihh and Ppr specifically in hypertrophic chondrocytes (HCs) and will dissect the mode of action of the Ihh and PPR pathways in HCs, in vivo, in transgenic and conditional knockout mice, Specially we will: 1) Investigate whether abnormal Ihh signaling and /or processing in 13del HCs is a primary cause of the differentiation abnormality by a) ablating Smoothened (Smo), the transducer of Ihh signaling in 13 del and wild-type HCs and b) over-expressing either Ihh or a non-secreted and unprocessed form of Ihh in normal HCs. 2) By the same rationale, study the role of the PTHrP/PPR pathway by ablating PPR in 13 del and wild-type HCs.


Project Title:Functional and genetic analyses of the role of type IIA procollagen in morphogenesis and as a regulator of BMP and TGF(Beta) signaling
Investigator(s):Cheah KSE
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2004
Abstract:
Refer to hard copy


Project Title:Strategic research theme of development and reproduction under the strategic research area of health development framework and seed funding proposal
Investigator(s):Cheah KSE, Ho PC, Wong WT, Sham PC, Chin FYL, Chan BP
Department:Biochemistry
Source(s) of Funding:Seed Funding for Strategic Research Theme
Start Date:05/2005
Abstract:
To promote and facilitate partnership that are highly interactive, collaborative and multidisciplinary; to achieve research excellence in the fields of development and reproduction and, through their translation, better treatment and prevention of diseases; to raise community awares and understanding of important issues and advances concerning healthy development and reproduction and treatment of diseases.


List of Research Outputs

Au Y.K., Wynn S.L., Cheah K.S.E. and Cheung K.M.C., Cre expression in the node and notochord in transgenic mice driven by FOXA2 minimal nothchord element, 51st Annual Meeting of the Orthopaedic Research Society, Washington DC, USA, February 20-23, 2005.
Au Y.K., Cheung K.M.C. and Cheah K.S.E., In vivo analysis of SOX9 by conditionally knock-in of a mutant Sox9, Sox9Y440X, in the notochord., Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Au Y.K., Wynn S.L., Cheah K.S.E. and Cheung K.M.C., The use of a mouse model to understand the pathogenesis of campomelic dysplasia caused by the human mutation, Sox9Y440X, 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Chan D., Yang L., Hui W.S., Chan W.C.W., Huang J., Shum D.K.Y., Cheung K.M.C., Luo Z.J. and Cheah K.S.E., A splicing mutation in the ext2 gene affects messenger RNA stability in a family with hereditary multiple exostoses, XIXth Meeting of the Federation of the European Connective Tissue Societies, Taormina-Giardini Noxos, Italy. 9-13 July 2004.
Chan K.W., Wong R.W.C., Mak K.K.L., Ng P.K.M., Mak H.S., Cheah K.S.E. and Chan S.Y., Functions of Epidermal Growth Factor Revealed by Analysis of Transgenic Mice, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 11.
Chan W.Y., Cheah K.S.E., Ng V.C.W., Murphy G. and Chan D., Mouse model with impaired collagen X degradation at the chondro-osseous junction, Gordon Research Conference: Biology and Pathology of Cartilage. Il Ciocco, Barga, Italy June 2005.
Cheah K.S.E., 16th International Congress of the IFAA, Kyoto, Japan, 22-27 August 2004. 2004.
Cheah K.S.E., An essential role for Sox2 in sensory organ development in the mammalian inner ear., Asia-Pacific Developmental biology Research Symposium, Kobe, Japan, 8-9 November 2004.
Cheah K.S.E., An essential role for Sox2 in sensory organ development in the mammalian inner ear, The Japanese Society of Developmental Biologists - Asia-Pacific Developmental Biology Research Symposium, Kobe, Japan, 8-9 November 2004. 2004.
Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., So C.L., Kwan K.M., Hunziker E.B., Yamada Y., Bateman J.F., Cheung K.M.C. and Chan D., Chondrocytes survive ER stress caused by unfolded proteins via re-programming, HGM2005 HUGO's 10th Human Genome Meeting, Kyoto, Japan 18-21 April 2005.
Cheah K.S.E., Development genomics and skeletal research, Hong Kong Institution of Science 2004 Annual Conference "Science Advancement in Hong Kong", 31 October 2004 Hong Kong. 2004.
Cheah K.S.E., Developmental genomics and skeletal research, Hong Kong Institution of Science, 12th Annual Conference 2004 on Science Advancement in Hong Kong. 2004.
Cheah K.S.E., Genetic analyses of matrix protein function and terminal differentiation of hypertrophic chondrocytes. , 16th International Congress of the International Federation of Association of Anatomists, 22-27 August 2004, Kyoto, Japan . 2004, 79: 160.
Cheah K.S.E., Wong S.Y.Y., Zhang J.C.L., Leung W.L., Kung H. and Tam P.P.L., Genetic analysis of the role of procollagen IIA as an extracellular regulator of BMP signaling, Frontiers in Biomedical Research, HKU 2004, 3 December 2004 Hong Kong. 2004.
Cheah K.S.E., Niewiadomska A.K., Dung W.F., Lau J.Y.B., Hunziker E., Aszodi A., Gustafsson E., Yamada Y., Zhou Z., Tryggvason K. and Chan D., Perlecan compartmentalizes collagen X in the mammalian growth plate., XIXth meeting of Federation of the European Connective Tissue Societies: Taormina-Giardia Naxos, Italy July 2004.
Cheah K.S.E., Procollagen IIA as an extracellular regulator of BMP signaling, The Joint Seminar of CDB & the university of Hong Kong, Center for Developmental Biology, Kobe, Japan, 15 April 2005. 2005.
Cheah K.S.E., Kiernan A., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D...M. and Lovell-Badge R.H., Sox2 is requured for sensory organ development in the inner ear, Cold Spring Harbor Meeting on Mouse Molecular Genetics, 1-5 September 2004.
Cheah K.S.E., The Mouse as a Measure of Man in Health and Disease, Science Forum of “Science Alive. DNA explore”organized by British Council, Science Museum, Hong Kong, 17 March 2005 . 2005.
Cheah K.S.E., Uncovering deafness genes: Sox2 is essential for sensory organ development in the inner ear., The 3rd HK Medical Genetics Conference, Hong Kong, 8-10 April, 2005.
Gao B., Cheah K.S.E., He L. and Chan D., Delayed endochondral ossification and digit joint formation in mice expressing a brachydactyly type A1 mutation in Indian Hedgehog, HGM2005 Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005 . 2005.
Huang J., Leong M.K., Watt R.M., Tsang A.C.C., Danchin A.L.M., Cheah K.S.E. and Liu D., Recombinogenic TaggingReveals Dynamic Compartmentalization of the Escherichia Coli Proteome, 2nd International Conference on Pathways, Networks, and Systems: Theory and Experiments, October 15-20, 2004, Crete, Greece. 2004.
Huen M.S.Y., Li X.T., Lu L., Watt R.M., Liu D., Cheah K.S.E. and Huang J., Unraveling the molecular basis of the bacteriophage lambda-encoded recombination system in E. coli, Keystone Symposia , Keystone, Colorado, USA, 5-11 January 2005.
Jim J.J.T., Chan D., Cheah K.S.E., Luk K.D.K. and Cheung K.M.C., Isolation and detection of type IX collagen from adult human intervertebral disc, 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Sham P.C., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., The TRP2 allele of collagen IX as an age-dependent risk factor for the development and severity of intervertebral disc degeneration, Asia Pacific Orthopaedic Association 14th Triennial Congress, 5-10 September 2004.
Kiernan A.E., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D.M., Teasse C., Lovell-Badge R.H., Steel K.P. and Cheah K.S.E., Sox2 is required for sensory organ development in the mammalian inner ear, Nature. 2005, 434: 0131-1035.
Lee Y.F., Pelling A.L., Leung K.K.H. and Cheah K.S.E., The molecular bases underlying the inner ear phenotypes of mouse mutants, yellow submarine (YSB)and light coat and circling (LCC), 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Leung K.K.H. and Cheah K.S.E., Sox2 is Required for Sensory Organ Development in the Inner Ear., Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Leung Y.L., Dung W.F., Lau J.Y.B., Leung K.K.H. and Cheah K.S.E., Identifying transcriptional regulatory elements directing hypertrophic chondrocyte-specific expression of Col10a1, 10th SCBA International Symposium, 18-23 July 2004.
Li J. and Cheah K.S.E., The Role of Indian Hedgehog in Chondrocyte Hypertrophy , Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D., Koopman P., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, Annual meeting of the international society for computational biology 2005. Michigan June 25-29 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D.A.G.M.A.R., Koopman P.E.T.E.R., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, ISMB Conerence 2005, 13th Annual International Conference on Intelligent Systems for Molecular Biology. Detroit Michigan, 25-29 June 2005.
Mak A.C.Y., Cheah K.S.E. and Smith D.K., Bioinformatics studies of gene regulation of SOX9 and the SOX family, 9th Research Postgraduate Symposium, HKU, 4 December 2004.
Pelling A.L., Kierman A., Leung K.K.H., Tang A.S.P., Bell D...M., Lovell-Badge R., Steel K. and Cheah K.S.E., Sox2 is required for sensory organ development in the inner ear, HGM2005 Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005 . 2004.
Song Y., Cheung K.M.C., Karppinen J., Ho D.W.H., Yip S...P., Leong J...C...Y., Ott J., Luk K.D.K., Cheah K.S.E., Sham P.C. and Chan D., Association studies of intervertebral disc disease with genes in the aggrecan degradation pathway, HUGO 2005: Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005.
Tai C.P., Wynn S.L. and Cheah K.S.E., In Vivo Analysis of Specificity of Target Gene Transactivation by SOX9 , The 4th Annual Australian Developmental Biology Workshop30th September - 4th October 2004 Rottnest Island, Perth, Western Australia . 2004.
Tai C.P. and Cheah K.S.E., In vivo analysis of the transactivation specificity of group E SOX proteins, 13th conference of the international society of differentiation Sheraton Waikiki, Honolulu, HawaiiSeptember 5-9, 2004. 2004.
Tai C.P. and Cheah K.S.E., In vivo analysis of the transactivation specificity of group E SOX proteins, 9th Research Postgraduate Symposium, HKU, 4 December 2004.
Tai C.P. and Cheah K.S.E., In vivo analysis of the transactivation specificity of group E SOX proteins, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Wilson R., Freddi S., Chan D., Cheah K.S.E. and Bateman J.F., Misfolding of collagen X chains harboring schmid metaphyseal chondrodysplasia mutations results in aberrant disulfide bond formation, intracellular retention, and activation of the unfolded protein response., Journal of Biological Chemistry. 2005, 22;280(16): 15544-52.
Wong E.Y.M., Cheah K.S.E. and Sham M.H., Ectopic expression of Hoxb3 in otic vesicle causes abnormal ear development, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interaction of Hoxb3 and Hoxb1 in hindbrain patterning revealed by ectopic expression of Hoxb3 in rhombomere 4 , 17 Annual Meeting on Mouse Molecular Genetics, Cold Spring Harbor Laboratory, New York, 1-5 September 2004.
Wu X.S., Xin L., Shang X.Y., Lu L., Watt R.M., Cheah K.S.E., Huang J., Liu D. and Liang C.C., Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression, Proceedings of the National Academy of Science. 2005, 102(7): 2508-2513.
Yang L. and Cheah K.S.E., Characterizing the re-activated Ihh singling in 13del programming chondrocytes, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Yang L., Chan D. and Cheah K.S.E., Genetic analyses of terminal differentiation of hypertrophic chondrocytes, 9th Research Postgraduate Symposium, HKU, December 4, 2004.


Researcher : Cheng LYL

Project Title:A concordance study of the importance of autosomal short tandem repeat (STR) and Y-chromosome STR in the Chinese community
Investigator(s):Cheng LYL, Tan-Un KC
Department:Biochemistry
Source(s) of Funding:Small Project Funding
Start Date:11/2002
Abstract:
In recent years, Short Tandem repeat (STR) and microsatellite analyses offer invaluable evidences in forensic science casework in court. Despite the well established database in the US forensic system, an Asian population database urgently needed to be established. In this research study, we use the STR markers that suit the American Combined DNA index system (CODIS) required by FBI to analyse Asian Chinese samples. Hence, the frequency of the allele for each locus can be calculated statistically, and stored for the use of the Chinese community.





Researcher : Cheng YW

List of Research Outputs

Cheng Y.W., Chan W.C.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse expressing mutant type X collagen, 51st Annual Meeting of the Orthopaedic Research Society, Washington DC, USA, February 20-23, 2005.
Cheng Y.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse model expressing mutant type X, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Cheung MS

List of Research Outputs

Ma R.Y.M., Tong H.K., Cheung M.S., Tsang A.C.C., Leung G.W.Y. and Yao K.M., Journal of Cell Science, Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. 2004, 118: 000-000.


Researcher : Chin KT

List of Research Outputs

Chin K.T. and Jin D., Development Of An Effective Method Totag Escherichia Coli Chromosomal Genes Based On Recombineering, The 8thEvolutionary Biology Meeting, Marseilles, France, Sept. 2004.. 2004.
Chin K.T., Functional characterization of the liver-enriched transcription factor CREB-H, PhD thesis. 2005.
Chin K.T. and Jin D., Human T-cell leukemia virus oncoprotein Tax represses nuclear receptor-dependent transcription by targeting coactivator TAX1BP1, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Chin K.T. and Jin D., Identification and characterization of a novel liver-enriched transcription factor of the BZIP family, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.
Chin K.T., Zhou H., Wong C.M., Lee M.F., Chan C.P., Qiang B.Q., Yuan J.G., Ng I.O.L. and Jin D., The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma, Nucleic Acids Research. 2005, 33(6): 1859-1873.
Chin K.T. and Jin D., The liver-enriched transcription factor creb-H is a growth suppressor under-expressed in hepatocellular carcinoma, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Ching Y.P., Chun C.S., Chin K.T., Zhang Z.Q., Jeang K.T. and Jin D., Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex, Retrovirology. 2004, 1(18): 1-12.


Researcher : Ching YP

Project Title:The functional characterisation of Pak5 and caspase 4 interaction- a role in apoptosis
Investigator(s):Ching YP
Department:Pathology
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Abstract:
To confirm the interaction of Pak5 and caspase 4 using three independent assays, including co-immunoprecipitation, co-immunostaining and GST affinity pull down assays; to map the minimal binding region of these two proteins and develop functional assays to assess the functional outcome of this interaction; to determine if Pak5 plays a role in apoptosis by regulating the caspase 4.


Project Title:Roles and regulation of group II p21-activated protein kinases:-implications in cancer metastasis
Investigator(s):Ching YP, Jin D, Ng IOL
Department:Pathology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2005
Abstract:
To study: (1) Characterisation of the interaction between Pak5 and NM23 i) co-immunoprecipitation of Pak5 adn NM23 ii) defining the binding domain between Pak 5 and NM23 iii) xploring the interaction between Pak4 adn NM23. (2) Impact of Pak5-NM23 interaction in the biochemical properties of Pak5 and NM23 i) nucleotide diphosphate kinase activity ii) GTPase activating activity iii) in vitro kinase activity iv) subcellular localisation. 3) Roles of PakII in cancer metastasis using HCC as a model i) expression profile of Pak4 in HCC ii) clinicopathological analysis iii) cell invasion assay.


Project Title:Roles and regulation of group II p21-activated protein kinases:-implications in cancer metastasis
Investigator(s):Ching YP
Department:Pathology
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
Refer to hard copy





Researcher : Choy EYW

List of Research Outputs

Choy E.Y.W. and Jin D., Construction of new DNA vectors based on promoters of Epstein-Barr virus-encoded small RNAs for expression of small hairpin RNAs in mammalian cells, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005.
Choy E.Y.W. and Jin D., Development of novel viral promoter-driven DNA vectors for expression of shRNAs to mediate RNA interference in mammalian cells, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Choy E.Y.W. and Jin D., Development of novel viral promoter-driven DNA vectors for expression of siRNAs to mediate RNA interference in mammalian cells, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.
Jin D., Kok K.H. and Choy E.Y.W., 10th SCBA International Symposium, Beijing, China, 18-23 July 2004, Rational design and development of small interfering RNAs and small hairpin RNAs for inhibition of hepatitis C virus replication. 2004.
Kok K.H., Choy E.Y.W., Ching Y.P. and Jin D., Cell-to-cell spreading of RNA interference in mammalian cells, The ASCB 44th Annual Meeting, Washington, DC, USA, 4-8 December 2004.


Researcher : Chun CS

List of Research Outputs

Ching Y.P., Chun C.S., Chin K.T., Zhang Z.Q., Jeang K.T. and Jin D., Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex, Retrovirology. 2004, 1(18): 1-12.
Chun C.S. and Jin D., Transcriptional regulation of mitotic checkpoint gene MAD1by p53, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Kok K.H., Chun C.S. and Jin D., Identification and characterization of TXBP151, a novel cellular target of HTLV-ITAX, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Chun CS

List of Research Outputs

Ching Y.P., Chun C.S., Chin K.T., Zhang Z.Q., Jeang K.T. and Jin D., Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex, Retrovirology. 2004, 1(18): 1-12.
Chun C.S. and Jin D., Transcriptional regulation of mitotic checkpoint gene MAD1by p53, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Kok K.H., Chun C.S. and Jin D., Identification and characterization of TXBP151, a novel cellular target of HTLV-ITAX, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Dung WF

List of Research Outputs

Cheah K.S.E., Niewiadomska A.K., Dung W.F., Lau J.Y.B., Hunziker E., Aszodi A., Gustafsson E., Yamada Y., Zhou Z., Tryggvason K. and Chan D., Perlecan compartmentalizes collagen X in the mammalian growth plate., XIXth meeting of Federation of the European Connective Tissue Societies: Taormina-Giardia Naxos, Italy July 2004.
Leung Y.L., Dung W.F., Lau J.Y.B., Leung K.K.H. and Cheah K.S.E., Identifying transcriptional regulatory elements directing hypertrophic chondrocyte-specific expression of Col10a1, 10th SCBA International Symposium, 18-23 July 2004.


Researcher : Gao B

List of Research Outputs

Gao B., Cheah K.S.E., He L. and Chan D., Delayed endochondral ossification and digit joint formation in mice expressing a brachydactyly type A1 mutation in Indian Hedgehog, HGM2005 Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005 . 2005.


Researcher : Garcia-Barcelo MM

Project Title:Role of RET regulatory polymorphisms in Hirschsprung's disease
Investigator(s):Garcia-Barcelo MM, Tam PKH, Ganster RW
Department:Surgery
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Abstract:
To identify and functionally test a RET susceptibility locus which may predispose to disease more than 60% of the Chinese HSCR patients and to replicate our findings in an extended sample.


Project Title:RET regulatory polymorphisms and susceptibility to Hirschsprung's disease
Investigator(s):Garcia-Barcelo MM, Tam PKH, Ganster RW, Sham P.C.
Department:Surgery
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2004
Abstract:
To study the effect of the RET promoter SNPs on transcription, independently and in conjection with other 5' cis-regulatory elements; to identify the transcription factor functioning at the SNPs site; to investigate whether there is functional and/or physical interactions between PHOX2B and the RET promoter; to test our genetic observations on a larger sample of Chinese HSCR patients; to investigate whether there is association between the transmission of HSCR-susceptibility RET haplotypes and PHOX2B-associated polymorphisms in affected individuals.


Project Title:RET regulatory polymorphisms and susceptibility to Hirschsprung's disease
Investigator(s):Garcia-Barcelo MM
Department:Surgery
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
Refer to hard copy


Project Title:Study of the molecular basis of anorectal malformations
Investigator(s):Garcia-Barcelo MM, Tam PKH, Lui VCH
Department:Surgery
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:01/2005
Abstract:
refer to hard copy proposal





Researcher : Ho DWH

List of Research Outputs

Song Y., Cheung K.M.C., Karppinen J., Ho D.W.H., Yip S...P., Leong J...C...Y., Ott J., Luk K.D.K., Cheah K.S.E., Sham P.C. and Chan D., Association studies of intervertebral disc disease with genes in the aggrecan degradation pathway, HUGO 2005: Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005.


Researcher : Huang J

Project Title:Identification of myosin VA interacting proteins
Investigator(s):Huang J, Wong NS, Pearlman R.E., Siu M.K.W.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2001
Abstract:
To use biochemical, molecular biology and cell biology techniques to evaluate the candidates that are likely to be the proteins mediating MyoVA binding to the cargoes; to identify more MCP candidates as well as other proteins that are important for MyoVA function.


Project Title:RNA-DNA hybrid oligonucleotide mediated site-specific repair and gene therapy
Investigator(s):Huang J, Liu DP
Department:Biochemistry
Source(s) of Funding:Matching Fund for Hi-Tech Research and Development Program of China (863 Projects)
Start Date:01/2003
Abstract:
To establish the reporter system for RDO and SSO mediated mutagenesis in mouse ES cells; to investigate the methods to improve the mutagenesis efficiency of RDO- and SSO-mediated mutagenesis; to study the mechanisms underlying SSO-mediated mutagenesis.


Project Title:DNA recombineering mediated by single stranded oligonucleotides
Investigator(s):Huang J, Cheah KSE, Watt RM, Liu DP
Department:Biochemistry
Source(s) of Funding:NSFC/RGC Joint Research Scheme
Start Date:03/2003
Abstract:
To understand detailed biophysical characterization o the [beta] protein; to identify endogenous proteins essential for Red-mediated recombination in E. coli; to introduce [beta] into mammalian cell lines; to study the mechanism of SSO-mediated mutagenesis in mammalian cells.


Project Title:Development of high throughput screen for the discovery of SARS coronavirus helicase inhibitors
Investigator(s):Huang J, Sun H, Poon LLM, Tanner JA, Watt RM,
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:10/2003
Completion Date:09/2004
Abstract:
To carry out biochemical characterization of the putative SARS coronavirus helicase; to evaluate known helicase inhibitors of their efficacy on SARS-CoV nsp10 protein; to develop high throughput assay for the screening of potential helicase inhibitors.


Project Title:Characterization of the molecular scaffolds in rab27a and myosin va-mediated vesicular transport
Investigator(s):Huang J, Sun H, Miller A
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Completion Date:01/2005
Abstract:
To characterize the vesicles transported by brain Myo Va isoform in Purkinje neurons; to develop a proteomic interactive map for MyoVa isoforms and Rab27 a across range of tissues; to elucidate the mechanism of scaffold assembly/disassembly.


Project Title:Helicases as antiviral drug targets
Investigator(s):Huang J, Zheng B, Sun H
Department:Biochemistry
Source(s) of Funding:Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:06/2004
Abstract:
Refer to hard copy


Project Title:Development of a novel system for recombinant protein production
Investigator(s):Huang J, Danchin A.L.M.
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Applied Research
Start Date:10/2004
Abstract:
To study in silicon identification of genetic elements necessary for high level protein production; to develop an efficient and versatile recombinant expression system for the expression of recombinant proteins; to carry out genetic modification of P. haloplanktis TAC125 for the improvement of protein production.


Project Title:Development of an Efficient Recombinant Protein Expression System in Pseudoalteromonas haloplanktis TAC125
Investigator(s):Huang J, Danchin A.L.M.
Department:Biochemistry
Source(s) of Funding:France/Hong Kong Joint Research Scheme - Travel Grants
Start Date:01/2005
Abstract:
In silico identification of cold-adapted promoters, strongly and tightly regulated by simple environmental changes, genetic modifications necessary to improve protein production; development of an efficient and versatile recombinant expression system for the expression of recombinant proteins; genetic modification of Pesudoalteromonas haloplanktis TAC125 for the improvement of protein production.


Project Title:Functions of the ubiquitously expressed kinesin in Purkinje neurons
Investigator(s):Huang J
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
Refer to hard copy


Project Title:Functions of the ubiquitously expressed kinesin in Purkinje neurons
Investigator(s):Huang J, Yip HKF, Wu W, Mugnaini E., Copeland N.G., Jenkins NA
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2005
Abstract:
To determine the KhcU mutation effect on overall cerebellum development; to determine whether PC can project their processes to the target area; determine the localization of ER, Golgi complex, mitochondria, synaptic vesicles and lysosomes; to determine whether KhcU-mediated transport is required for normal MyoVA distribution and neurite out growth; to determine the ultrastructures of KhcU-deficiency PC.


Project Title:Biochemical characterization of a putative helicase from a novel coronavirus that causes pneumonia
Investigator(s):Huang J, Yuen KY
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2005
Abstract:
refer hard copy proposal


List of Research Outputs

Bernini A., Spiga O., Ciutti A., Chiellini S., Bracci L., Yan X.Y., Zheng B.J., Huang J., He M.L., Song H.D., Hao P., Zhao G.P. and Niccolai N., Prediction of Quaternary Assembly of SARS CoronavirusPeplomer, Biochemical and Biophysical Research Communications. 2004, 325(4): 1210-1214.
Bernini A., Spiga O., Ciutti A., Chiellini S., Bracci L., Yan X.Y., Zheng B., Huang J., He M.L., Song H.D., Hao P., Zhao G.P. and Niccolai N., Prediction of quaternary assembly of SARS coronavirus peplomer, Biochemical and Biophysical Research Communications. 2004, 325: 1210-1214.
Chan D., Yang L., Hui W.S., Chan W.C.W., Huang J., Shum D.K.Y., Cheung K.M.C., Luo Z.J. and Cheah K.S.E., A splicing mutation in the ext2 gene affects messenger RNA stability in a family with hereditary multiple exostoses, XIXth Meeting of the Federation of the European Connective Tissue Societies, Taormina-Giardini Noxos, Italy. 9-13 July 2004.
Court D., Li X., Huang J., Constantino N. and Liu D., Host Cells Deficient for Mismatch Repair, US Patent WO2004106513. 2004.
He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Huang J., Zhang X., Chen B., Ng A.H.L., Tanner J.A., Tay D.K.C., So K.F., Rachel R.A., Copeland N.G. and Jenkins N.A., Cre-transgenic mouse line for conditional gene knockout in retinal rod bipolar cells , INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 2005, 46: 3111S.
Huang J., Tanner J.A. and Zhang X., METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES, US Patent WO2005010179. 2005.
Huang J., Leong M.K., Watt R.M., Tsang A.C.C., Danchin A.L.M., Cheah K.S.E. and Liu D., Recombinogenic TaggingReveals Dynamic Compartmentalization of the Escherichia Coli Proteome, 2nd International Conference on Pathways, Networks, and Systems: Theory and Experiments, October 15-20, 2004, Crete, Greece. 2004.
Huen M.S.Y., Kung H. and Huang J., Probing the mechanistic basis of the bacteriophage lambda-encoded recombination system in E. Coli, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Huen M.S.Y., Li X.T., Lu L., Watt R.M., Liu D., Cheah K.S.E. and Huang J., Unraveling the molecular basis of the bacteriophage lambda-encoded recombination system in E. coli, Keystone Symposia , Keystone, Colorado, USA, 5-11 January 2005.
Leong M.K., Danchin A.L.M. and Huang J., Development of an Effective Method to Tag Escherichia ColiChromosomal Genes Based on Recombineering, 8th Evolutionary Biology Meeting, September 22-24, 2004, Marseille, France . 2004.
Liu B., Chan J.K.M., Huang J. and Zhou Z., Premature aging and genomic instability in mice lacking zmpste24, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Ng A.H.L. and Huang J., Characterization of the specificity of Cre expression in cerebellar Purkinje cells, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Ng A.H.L., Zhang X. and Huang J., Characterize the specificity of CRE expression in cerebellar purkinje cells, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
O'Sullivan T.N., Wu X.S., Rachel R.A., Huang J., Swing D.A., Matesic L.E., Hammer J.A., Copeland N.G. and Jenkins N.A., DSU Functions in a MYO5A-Independent Pathway to Suppress the Coat Color of Dilute Mice, Proceedings of the National Academy of Sciences. 2004, 101(48): 16831-16836.
Sun H., Ge R., Watt R.M., He Q. and Huang J., Towards the understanding of nickel trafficking in helicobacter pylori: Expression and characterization of a his-rich protein, HPN, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.
Tanner J.A., Watt R.M., Kao R.Y.T. and Huang J., Targeting the SARS Coronavirus Helicase - Three Approaches to Inhibitor Development, FASEB Journal. 2005, 19: 217.9.
Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.
Tyska M.J., Mackey A.T., Huang J., Copeland N.G., Jenkins N.A. and Mooseker M.S., Myosin-1a is critical for normal brush border structure and composition, Molecular Biology of the Cell. 2005, 16: 2443-2457.
Wong Q.N.Y., Ng V.C.W., Lin M.C., Huang F.Y., Chan D. and Huang J., Efficient and seamless DNA recombineering using a thymidylate synthase a selection system in Escherichia coli, Nucleic Acids Research. 2005, 33(6): 2-9.
Wu X.S., Xin L., Shang X.Y., Lu L., Watt R.M., Cheah K.S.E., Huang J., Liu D. and Liang C.C., Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression, Proceedings of the National Academy of Science. 2005, 102(7): 2508-2513.
Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.


Researcher : Huang J

List of Research Outputs

Bernini A., Spiga O., Ciutti A., Chiellini S., Bracci L., Yan X.Y., Zheng B.J., Huang J., He M.L., Song H.D., Hao P., Zhao G.P. and Niccolai N., Prediction of Quaternary Assembly of SARS CoronavirusPeplomer, Biochemical and Biophysical Research Communications. 2004, 325(4): 1210-1214.
Bernini A., Spiga O., Ciutti A., Chiellini S., Bracci L., Yan X.Y., Zheng B., Huang J., He M.L., Song H.D., Hao P., Zhao G.P. and Niccolai N., Prediction of quaternary assembly of SARS coronavirus peplomer, Biochemical and Biophysical Research Communications. 2004, 325: 1210-1214.
Chan D., Yang L., Hui W.S., Chan W.C.W., Huang J., Shum D.K.Y., Cheung K.M.C., Luo Z.J. and Cheah K.S.E., A splicing mutation in the ext2 gene affects messenger RNA stability in a family with hereditary multiple exostoses, XIXth Meeting of the Federation of the European Connective Tissue Societies, Taormina-Giardini Noxos, Italy. 9-13 July 2004.
Court D., Li X., Huang J., Constantino N. and Liu D., Host Cells Deficient for Mismatch Repair, US Patent WO2004106513. 2004.
He M.L., Zheng B., Guan Y., Chen Y., Wong K.L., Huang J., Peng Y., Yuen K.Y. and Kung H., The inhibitory effects of siRNAS targeting the replicase or structural genes of the sars-associated coronavirus (SCOV) on viral replication, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Huang J., Zhang X., Chen B., Ng A.H.L., Tanner J.A., Tay D.K.C., So K.F., Rachel R.A., Copeland N.G. and Jenkins N.A., Cre-transgenic mouse line for conditional gene knockout in retinal rod bipolar cells , INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 2005, 46: 3111S.
Huang J., Tanner J.A. and Zhang X., METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES, US Patent WO2005010179. 2005.
Huang J., Leong M.K., Watt R.M., Tsang A.C.C., Danchin A.L.M., Cheah K.S.E. and Liu D., Recombinogenic TaggingReveals Dynamic Compartmentalization of the Escherichia Coli Proteome, 2nd International Conference on Pathways, Networks, and Systems: Theory and Experiments, October 15-20, 2004, Crete, Greece. 2004.
Huen M.S.Y., Kung H. and Huang J., Probing the mechanistic basis of the bacteriophage lambda-encoded recombination system in E. Coli, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Huen M.S.Y., Li X.T., Lu L., Watt R.M., Liu D., Cheah K.S.E. and Huang J., Unraveling the molecular basis of the bacteriophage lambda-encoded recombination system in E. coli, Keystone Symposia , Keystone, Colorado, USA, 5-11 January 2005.
Leong M.K., Danchin A.L.M. and Huang J., Development of an Effective Method to Tag Escherichia ColiChromosomal Genes Based on Recombineering, 8th Evolutionary Biology Meeting, September 22-24, 2004, Marseille, France . 2004.
Liu B., Chan J.K.M., Huang J. and Zhou Z., Premature aging and genomic instability in mice lacking zmpste24, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Ng A.H.L. and Huang J., Characterization of the specificity of Cre expression in cerebellar Purkinje cells, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Ng A.H.L., Zhang X. and Huang J., Characterize the specificity of CRE expression in cerebellar purkinje cells, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
O'Sullivan T.N., Wu X.S., Rachel R.A., Huang J., Swing D.A., Matesic L.E., Hammer J.A., Copeland N.G. and Jenkins N.A., DSU Functions in a MYO5A-Independent Pathway to Suppress the Coat Color of Dilute Mice, Proceedings of the National Academy of Sciences. 2004, 101(48): 16831-16836.
Sun H., Ge R., Watt R.M., He Q. and Huang J., Towards the understanding of nickel trafficking in helicobacter pylori: Expression and characterization of a his-rich protein, HPN, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.
Tanner J.A., Watt R.M., Kao R.Y.T. and Huang J., Targeting the SARS Coronavirus Helicase - Three Approaches to Inhibitor Development, FASEB Journal. 2005, 19: 217.9.
Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.
Tyska M.J., Mackey A.T., Huang J., Copeland N.G., Jenkins N.A. and Mooseker M.S., Myosin-1a is critical for normal brush border structure and composition, Molecular Biology of the Cell. 2005, 16: 2443-2457.
Wong Q.N.Y., Ng V.C.W., Lin M.C., Huang F.Y., Chan D. and Huang J., Efficient and seamless DNA recombineering using a thymidylate synthase a selection system in Escherichia coli, Nucleic Acids Research. 2005, 33(6): 2-9.
Wu X.S., Xin L., Shang X.Y., Lu L., Watt R.M., Cheah K.S.E., Huang J., Liu D. and Liang C.C., Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression, Proceedings of the National Academy of Science. 2005, 102(7): 2508-2513.
Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.


Researcher : Huen MSY

List of Research Outputs

Huen M.S.Y., Kung H. and Huang J., Probing the mechanistic basis of the bacteriophage lambda-encoded recombination system in E. Coli, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Huen M.S.Y., Li X.T., Lu L., Watt R.M., Liu D., Cheah K.S.E. and Huang J., Unraveling the molecular basis of the bacteriophage lambda-encoded recombination system in E. coli, Keystone Symposia , Keystone, Colorado, USA, 5-11 January 2005.


Researcher : Jiang J

List of Research Outputs

Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.


Researcher : Jin D

Project Title:Implementation of theoretical and technical system for disease genomics
Investigator(s):Jin D
Department:Institute of Molecular Biology
Source(s) of Funding:Matching Fund for National Key Basic Research Development Scheme (973 Projects)
Start Date:08/2001
Abstract:
To study implementation of theoretical and technical system for disease genomics.


Project Title:Characterization of a novel centrosomal target of HTLV-I oncoprotein tax
Investigator(s):Jin D
Department:Institute of Molecular Biology
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:12/2001
Abstract:
To characterize a novel human centrosomal protein, which binds to HTLV-I oncoprotein Tax in yeast two-hybrid assay and co-immunoprecipitates with Tax from extracts of mammalian cells.


Project Title:Mitotic checkpoint and genomic stability in ovarian cancer
Investigator(s):Jin D
Department:Biochemistry
Source(s) of Funding:National Institutes of Health, US Department of Health and Human Services - General Award
Start Date:09/2002
Abstract:
To investigate the molecular basis of mitotic checkpoint in mammalian cells nad its relevance to genomic instability in ovarian cancer.


Project Title:Functional characterization of a novel liver-enriched transcription factor of the bZIP family
Investigator(s):Jin D, Chung SK, Ching YP, Ng IOL
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:12/2002
Abstract:
Previous study of the research team shows that LZIP-[beta] is a liver-enriched transcription factor implicated in tissue-specific expression of genes. In this study, the team will focus on four areas of research:- 1) biochemical and biological characterization of LZIP-[beta] transcript and protein in mammalian tissues and cells; 2) functional characterization of LZIP-[beta] as a novel CRE-binding transcription factor; 3) investigating the roles of LZIP-[beta] in liver-specific gene expression and cancer development; and 4) establishment of frog and mouse models for functional studies of LZIP-[beta].


Project Title:Development of novel nucleic acid therapeutics for viral infection and cancer
Investigator(s):Jin D, Chan LC, Sham MH, Ko CB, Tsao GSW, Smith DK, Lai CL, Ng IOL
Department:Biochemistry
Source(s) of Funding:Innovation and Technology Support Programme
Start Date:06/2003
Completion Date:05/2005
Abstract:
To further optimize the protocols for computer-aided design of siRNAs/ shRNAs and for mass production of siRNAs through in vitro transcription catalyzed by T7/SP6 RNA polymerase; to develop new and improved vectors for delivery of shRNA into human cells; to design, produce and test more than 50 siRNAs and shRNAs targeting hepatitis B and C viruses, lymphoma and leukemia, as well as liver cancer; to develop and further improve various indicator plasmid constructs, cell culture systems and animal models for evaluating the effectiveness of nucleic acid therapeutics on hepatitis, liver cancer, lymphoma and leukemia.


Project Title:Development of RNAi technology for inhibition of viral replication
Investigator(s):Jin D
Department:Biochemistry
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Abstract:
To use Sindbis virus as a model to systematically study RNAi-mediated inhibition of viral replication; to study the rules for selection of RNAi targets particularly effective for inhibition of viral replication in mammalian cells; to compare the effectiveness of siRNAs targeting UTR, non-structural (such as polymerase) or structural (such as capsid) regions.


Project Title:Roles and regulation of peroxiredoxins: from yeast to human
Investigator(s):Jin D
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:12/2003
Abstract:
To perform in-depth analyses on the transcriptional regulation of yeast PMP20; to study the structure-function relationship of yeast and human peroxiredoxins; to characterize the impact of binding to heme on Tsa1p/Tsa2p function; to investigate the roles of peroxiredoxins in cell physiology and pathology. Out focus will be on apoptosis, aging and cell proliferation.


Project Title:Roles and regulation of peroxiredoxins: from yeast to human
Investigator(s):Jin D
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:12/2003
Abstract:
To perform in-depth analyses on the transcriptional regulation of yeast PMP20; to study the structure-function relationship of yeast and human peroxiredoxins; to characterize the impact of binding to heme on Tsa1p/Tsa2p function; to investigate the roles of peroxiredoxins in cell physiology and pathology. Out focus will be on apoptosis, aging and cell proliferation.


Project Title:Regulatory roles for vault poly(ADP-ribose) polymerase in NF(KAPPA)B activation
Investigator(s):Jin D
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2004
Abstract:
Refer to hard copy


Project Title:Regulatory roles for vault poly(ADP-ribose) polymerase in NF[kappa]B activation
Investigator(s):Jin D, Yam JWP
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:03/2005
Abstract:
refer hard copy proposal


List of Research Outputs

Chan C.P., Siu K.L., Zheng B. and Jin D., Subcellular localization of sars coronavirus orf8 protein, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Cheung H.W., Jin D., Ling M.T., Wong Y.C., Wang Q., Tsao G.S.W. and Wang X., MAD2 expression induces chemosensitization to DNA damaging platin, in nasopharyngeal carcinoma cells, Proceeding of the American Association for Cancer Research, April 2005. 46: 1259 No. 5326.
Cheung H.W., Jin D., Ling M.T., Wong Y.C., Wang Q., Tsao G.S.W. and Wang X., Mitotic arrest deficient 2 expression induces chemosensitization to a DNA-damaging agent, cisplatin, in nasopharyngeal carcinoma cells, Cancer Research. 2005, 65(4): 1450-1458.
Cheung N., So C.W., Yam J.W.P., So C.K.C., Poon R.Y.C., Jin D. and Chan L.C., Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukemia, Biochemical Journal. 2004, 383(Pt 1): 27-35.
Chin K.T. and Jin D., Development Of An Effective Method Totag Escherichia Coli Chromosomal Genes Based On Recombineering, The 8thEvolutionary Biology Meeting, Marseilles, France, Sept. 2004.. 2004.
Chin K.T. and Jin D., Human T-cell leukemia virus oncoprotein Tax represses nuclear receptor-dependent transcription by targeting coactivator TAX1BP1, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Chin K.T. and Jin D., Identification and characterization of a novel liver-enriched transcription factor of the BZIP family, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.
Chin K.T., Zhou H., Wong C.M., Lee M.F., Chan C.P., Qiang B.Q., Yuan J.G., Ng I.O.L. and Jin D., The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma, Nucleic Acids Research. 2005, 33(6): 1859-1873.
Chin K.T. and Jin D., The liver-enriched transcription factor creb-H is a growth suppressor under-expressed in hepatocellular carcinoma, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Ching Y.P., Wong C.M., Jin D. and Ng I.O.L., Identification of a novel RHO GTPASE activating protein called DLC2, involved in hepatocellular carcinoma, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.
Choy E.Y.W. and Jin D., Construction of new DNA vectors based on promoters of Epstein-Barr virus-encoded small RNAs for expression of small hairpin RNAs in mammalian cells, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005.
Choy E.Y.W. and Jin D., Development of novel viral promoter-driven DNA vectors for expression of shRNAs to mediate RNA interference in mammalian cells, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Choy E.Y.W. and Jin D., Development of novel viral promoter-driven DNA vectors for expression of siRNAs to mediate RNA interference in mammalian cells, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.
Chun C.S. and Jin D., Transcriptional regulation of mitotic checkpoint gene MAD1by p53, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Jin D., Kok K.H. and Choy E.Y.W., 10th SCBA International Symposium, Beijing, China, 18-23 July 2004, Rational design and development of small interfering RNAs and small hairpin RNAs for inhibition of hepatitis C virus replication. 2004.
Kok K.H., Choy E.Y.W., Ching Y.P. and Jin D., Cell-to-cell spreading of RNA interference in mammalian cells, The ASCB 44th Annual Meeting, Washington, DC, USA, 4-8 December 2004.
Kok K.H., Chun C.S. and Jin D., Identification and characterization of TXBP151, a novel cellular target of HTLV-ITAX, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Leung T.H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Identification of multiple isoforms of DLC2, a novel tumor suppressor gene, and their functional characterization in hepatocellular carcinoma, 4th International Meeting of Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, December 2004 (Young Investigator Award). 2004.
Ng D.C.H., Jin D. and Ng I.O.L., Characterisation of Steroidogenic Acute Regulatory Protein Related Lipid Transfer Domain (START)domain in Deleted in Liver Cancer 2(DLC-2)., Society For Chinese Bioscientists in America, Beijing, July 2004 . 2004.
Ng D.C.H., Ching Y.P., Ng I.O.L. and Jin D., Functional characterization of a Novel RhoGAP protein deleted in liver cancer 2 (DLC2), 9th research postgraduate symposium, HKU, December 4, 2004.
Ng D.C.H., Ching Y.P., Chan S.F. and Jin D., Human T-cell leukemia virus type I oncoprotein tax targets the centrosome, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Siu K.L., Wong C.M. and Jin D., 10th SCBA International Symposium, Beijing, China, 18-23 July 2004, Peroxiredoxin-null yeast cells are genomically unstable. 2004.
Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Association of MAD2 Expression with Mitotic Checkpoint Competence in Hepatoma Cells, J. Biomed. Sci. . 2004, 11: 920-7.
Wang X., Wong Y.C. and Jin D., Mitotic checkpoint: a therapeutic target in the treatment of human cancer, Letters in Drug Design and Discovery. 2005, 2: 184-193.
Yam J.W.P., Jin D. and Chan L.C., Identification and characterization of EBP, a Novel EEN binding protein that inhibits ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.


Researcher : Kok KH

List of Research Outputs

Jin D., Kok K.H. and Choy E.Y.W., 10th SCBA International Symposium, Beijing, China, 18-23 July 2004, Rational design and development of small interfering RNAs and small hairpin RNAs for inhibition of hepatitis C virus replication. 2004.
Kok K.H., Choy E.Y.W., Ching Y.P. and Jin D., Cell-to-cell spreading of RNA interference in mammalian cells, The ASCB 44th Annual Meeting, Washington, DC, USA, 4-8 December 2004.
Kok K.H., Chun C.S. and Jin D., Identification and characterization of TXBP151, a novel cellular target of HTLV-ITAX, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Kwok JCF

List of Research Outputs

Kwok J.C.F., Ng K.Y., Zhang F., Chan Y.S. and Shum D.K.Y., Chondroitin sulfates restrict axonal growth along the projection path of vestibular nuclear neurons across the hindbrain of prenatal rats, Neuroscience Letters. 2004, 370 Suppl: S13–14.


Researcher : Lai WL

List of Research Outputs

Lai W.L. and Wong N.S., ROS Mediates 4HPR-induced Post-transcriptional Expression Of Gadd153 Gene, Free Radical Biology and Medicine. Elsevier, 2005, 38(12): 1585.
Lai W.L. and Wong N.S., Reactive oxygen species (ROS) regulates the expression of the growth-arrest and DNA damage inducible gene-153 at the post-transcriptional level, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Lai W.L. and Wong N.S., Reactive oxygen species (ROS) regulates the expression of the growth-arrest-and DNA-damage inducible gene at the post-transcriptional level, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.


Researcher : Lam VMS

Project Title:Structural NADP+, subunit interaction and other stability determinants in human glucose-6-phosphate dehydrogenase (G6PD) and deficient variants
Investigator(s):Lam VMS, Engel P.C., Adams M.J.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:11/2000
Completion Date:09/2005
Abstract:
Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a global and local of jaundice in newborns. The majority are usually asymptomatic but environmental agents such as the ingestion of fava bean or some Chinese herbal medicines can induce favism or haemolysis. The project aims to bioengineer change(s) in the amino acids, around the 'structural' NADP site, some of which correspond to naturally occurring Class 1 variants.


Project Title:The C-terminus of human glucose-6-phosphate dehydrogenase (h-G6PD): a structural and/or regulatory domain?
Investigator(s):Lam VMS, Engel P.C., Adams M.J.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:12/2002
Completion Date:09/2005
Abstract:
The project aims at examining the role of the aromatic amino acids in the C-terminus. The research team will create a series of mutations in each of these residues, express and purify these enzymes for assay of stability and NADP+ binding.


Project Title:A study on the roles of the amino acids involved in the catalytic efficiency of human glucose-6-phosphate dehydrogenase (G6PD)
Investigator(s):Lam VMS
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Abstract:
To construct recombinants which can express R72Q and R72A mutant enzymes, in different vectors; take experiment to express the enzymes under different conditions; to develop different strategies to purify the enzymes; ADP-sepharose column is likely to be ineffective; to obtain initial rate measurements and determine the kinetic and/or NADP+ binding constants of these enzymes.


Project Title:'Structural' NADP+ and conformation change accompanying Glucose-6-phosphate dehydrogenase (G5PD) activity
Investigator(s):Lam VMS, Au SWN
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:01/2005
Abstract:
refer to hard copy proposal


List of Research Outputs

Au W.Y., Ma E.S.K., Lam V.M.S., Chan L.C., Pang A.W.K. and Kwong Y.L., Glucose 6-phosphate dehydrogenase (G6PD) deficiency in elderly Chinese women heterozygous for G6PD variants, American Journal of Medical Genetics Part A. 2004, 129A(2): 208 - 211.
Kataka M., Gover S., Vandeputte-Rutten S., Lam V.M.S. and Adams M.J., Structural studies of glucose-6-phosphate and NADP + binding to human glucose-6=phosphate dehydrogenase , Acta Crystallographica . 2005, D61: 495-504.
Wang X., Enget P.A.U.L. .C. and Lam V.M.S., "Structural" NADP+ and the dimer interface: Detailed analyses of G6PD class I deficient mutants at R393, 9th research postgraduate symposium, HKU, December 4, 2004.
Wang X., Engel P...C... and Lam V.M.S., "Structural" NAPP+and G6PD Class 1 deficient mutants, 5th Human Genome Organization (HUGO) Pacific Meeting and 6thAsia-Pacific Conference on Human Genetics. November 17,2004 Singapore. 2004.


Researcher : Lau JYB

List of Research Outputs

Cheah K.S.E., Niewiadomska A.K., Dung W.F., Lau J.Y.B., Hunziker E., Aszodi A., Gustafsson E., Yamada Y., Zhou Z., Tryggvason K. and Chan D., Perlecan compartmentalizes collagen X in the mammalian growth plate., XIXth meeting of Federation of the European Connective Tissue Societies: Taormina-Giardia Naxos, Italy July 2004.


Researcher : Law ML

List of Research Outputs

Law M.L., Tsang W.H., Chan K.K. and Sham M.H., Abnormal enteric ganglia development in a SOX 10 mouse mutant generated by gene targeting, 9th Rearch Postgraduate Symposium, HKU4 December 2004.
Law M.L. and Sham M.H., Abnormal enteric ganglia development in a Sox10 mouse mutant generated by gene targeting, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Law M.L., Ngan E.S.W., Lui V.C.H., Tam P.K.H. and Sham M.H., The role of Sox 10 on survival and proliferation of enteric neural crest stem cells, 3rd Annual Meeting of International Society for Stem Cell Research.. 2005.


Researcher : Law ML

List of Research Outputs

Law M.L., Tsang W.H., Chan K.K. and Sham M.H., Abnormal enteric ganglia development in a SOX 10 mouse mutant generated by gene targeting, 9th Rearch Postgraduate Symposium, HKU4 December 2004.
Law M.L. and Sham M.H., Abnormal enteric ganglia development in a Sox10 mouse mutant generated by gene targeting, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Law M.L., Ngan E.S.W., Lui V.C.H., Tam P.K.H. and Sham M.H., The role of Sox 10 on survival and proliferation of enteric neural crest stem cells, 3rd Annual Meeting of International Society for Stem Cell Research.. 2005.


Researcher : Law SKF

List of Research Outputs

Law S.K.F., MPhil Thesis, The molecular consequences of Indian hedgehog mutations in distal digit patterning. 2004.


Researcher : Lee JSK

List of Research Outputs

Au W.Y. and Lee J.S.K., Imatinib mesylate (STI-571) and porphyria cutanea tarda in a Chinese patient, Haematologica. 2005, 90: ECR18.
Ip P., Wong V.C.N., Ho M.H.K., Lee J.S.K. and Wong W.H.S., Environmental mercury exposure in children: South China's experience, Pediatrics International. 2004, 46(6): 715-721.
Ip P., Wong V.C.N., Ho M.H.K., Lee J.S.K. and Wong W.H.S., Mercury Exposure in Children With Autistic Spectrum Disorder: Case-Control Study, Journal of Child Neurology. 2004, 19: 431-434.
Lee P.P.W., Poon G.W.K., Kwan E.Y.W., Wong K.Y., Chan K.W., Lee J.S.K., Tam S.C.F., Lau E., Tang M.H.Y., Gu X.F., Low L.C.K. and Cheung P.T., Antenatal Diagnosis and Postnatal Management of Tetrahydrobiopterin-deficient Hyperphenyalaninemia in a Hong Kong Chinese Infant, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 37.
Poon G.W.K., Mak C.M., Wong R.M.S., Wong K.Y., Yung T.C., Tam S., Siu S.T.S., Lee J.S.K. and Low L.C.K., Reversible Myocardial Damage in a Premature Infant with Carnitine-Acylcarnitine Translocase Deficiency, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 33.


Researcher : Lee YF

List of Research Outputs

Lee Y.F., Pelling A.L., Leung K.K.H. and Cheah K.S.E., The molecular bases underlying the inner ear phenotypes of mouse mutants, yellow submarine (YSB)and light coat and circling (LCC), 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.


Researcher : Leong JCY

List of Research Outputs

Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Sham P.C., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., The TRP2 allele of collagen IX as an age-dependent risk factor for the development and severity of intervertebral disc degeneration, Asia Pacific Orthopaedic Association 14th Triennial Congress, 5-10 September 2004.


Researcher : Leong MK

List of Research Outputs

Huang J., Leong M.K., Watt R.M., Tsang A.C.C., Danchin A.L.M., Cheah K.S.E. and Liu D., Recombinogenic TaggingReveals Dynamic Compartmentalization of the Escherichia Coli Proteome, 2nd International Conference on Pathways, Networks, and Systems: Theory and Experiments, October 15-20, 2004, Crete, Greece. 2004.
Leong M.K., Danchin A.L.M. and Huang J., Development of an Effective Method to Tag Escherichia ColiChromosomal Genes Based on Recombineering, 8th Evolutionary Biology Meeting, September 22-24, 2004, Marseille, France . 2004.
Leong M.K., PhD Thesis, Development of an effective method to tag escherichia COL1 chromosomal genes by recombineering. 2004.


Researcher : Leung GWY

List of Research Outputs

Ma R.Y.M., Tong H.K., Cheung M.S., Tsang A.C.C., Leung G.W.Y. and Yao K.M., Journal of Cell Science, Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. 2004, 118: 000-000.
Ma R.Y.M., Tsang A.C.C., Leung G.W.Y. and Yao K.M., The mitogen-activated protein kinase pathway promotes mitotic progression through the forkhead box transcription factor foxmi, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.


Researcher : Leung KKH

List of Research Outputs

Cheah K.S.E., Kiernan A., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D...M. and Lovell-Badge R.H., Sox2 is requured for sensory organ development in the inner ear, Cold Spring Harbor Meeting on Mouse Molecular Genetics, 1-5 September 2004.
Kiernan A.E., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D.M., Teasse C., Lovell-Badge R.H., Steel K.P. and Cheah K.S.E., Sox2 is required for sensory organ development in the mammalian inner ear, Nature. 2005, 434: 0131-1035.
Lee Y.F., Pelling A.L., Leung K.K.H. and Cheah K.S.E., The molecular bases underlying the inner ear phenotypes of mouse mutants, yellow submarine (YSB)and light coat and circling (LCC), 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Leung K.K.H. and Cheah K.S.E., Sox2 is Required for Sensory Organ Development in the Inner Ear., Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Leung Y.L., Dung W.F., Lau J.Y.B., Leung K.K.H. and Cheah K.S.E., Identifying transcriptional regulatory elements directing hypertrophic chondrocyte-specific expression of Col10a1, 10th SCBA International Symposium, 18-23 July 2004.
Pelling A.L., Kierman A., Leung K.K.H., Tang A.S.P., Bell D...M., Lovell-Badge R., Steel K. and Cheah K.S.E., Sox2 is required for sensory organ development in the inner ear, HGM2005 Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005 . 2004.
Yip J.W.S. and Leung K.K.H., The genetics of the degenerative intervertebral disc disease, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Leung WL

List of Research Outputs

Cheah K.S.E., Wong S.Y.Y., Zhang J.C.L., Leung W.L., Kung H. and Tam P.P.L., Genetic analysis of the role of procollagen IIA as an extracellular regulator of BMP signaling, Frontiers in Biomedical Research, HKU 2004, 3 December 2004 Hong Kong. 2004.


Researcher : Leung YL

List of Research Outputs

Leung Y.L., Dung W.F., Lau J.Y.B., Leung K.K.H. and Cheah K.S.E., Identifying transcriptional regulatory elements directing hypertrophic chondrocyte-specific expression of Col10a1, 10th SCBA International Symposium, 18-23 July 2004.


Researcher : Li J

List of Research Outputs

Li J. and Cheah K.S.E., The Role of Indian Hedgehog in Chondrocyte Hypertrophy , Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Li X

List of Research Outputs

Court D., Li X., Huang J., Constantino N. and Liu D., Host Cells Deficient for Mismatch Repair, US Patent WO2004106513. 2004.


Researcher : Liu B

List of Research Outputs

Liu B. and Zhou Z., Genomic instability caused by unprocessed prelamin A contributes to premature aging in mice lacking Zmpste24, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Liu B., Chan J.K.M., Huang J. and Zhou Z., Premature aging and genomic instability in mice lacking zmpste24, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Zhou Z., Wang J., Cao R., Morita H., Soininen R., Chan J.K.M., Liu B., Cao Y. and Tryggvason K., Impaired angiogenesis, delayed wound healing and retarded tumor growth in perlecan heparan sulfate-deficient mice, Cancer Research. 2004, 15;64(14): 4699-702.


Researcher : Liu D

List of Research Outputs

Court D., Li X., Huang J., Constantino N. and Liu D., Host Cells Deficient for Mismatch Repair, US Patent WO2004106513. 2004.
Huang J., Leong M.K., Watt R.M., Tsang A.C.C., Danchin A.L.M., Cheah K.S.E. and Liu D., Recombinogenic TaggingReveals Dynamic Compartmentalization of the Escherichia Coli Proteome, 2nd International Conference on Pathways, Networks, and Systems: Theory and Experiments, October 15-20, 2004, Crete, Greece. 2004.
Huen M.S.Y., Li X.T., Lu L., Watt R.M., Liu D., Cheah K.S.E. and Huang J., Unraveling the molecular basis of the bacteriophage lambda-encoded recombination system in E. coli, Keystone Symposia , Keystone, Colorado, USA, 5-11 January 2005.
Wu X.S., Xin L., Shang X.Y., Lu L., Watt R.M., Cheah K.S.E., Huang J., Liu D. and Liang C.C., Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression, Proceedings of the National Academy of Science. 2005, 102(7): 2508-2513.


Researcher : Liu H

List of Research Outputs

Liu H. and Shum D.K.Y., Chondroitin sulfate proteoglycans (CSPG) and hyaluronan at borders between astrocytes and schwann cells restrict neurite crossing., Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Liu H. and Shum D.K.Y., Chondroitin sulfate proteoglycans (CSPG) produced by schwann cells in astrocyte-schwann cell cocultures limit neurite extension and crossing of cellular boundaries, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.


Researcher : Lovell-Badge RH

List of Research Outputs

Cheah K.S.E., Kiernan A., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D...M. and Lovell-Badge R.H., Sox2 is requured for sensory organ development in the inner ear, Cold Spring Harbor Meeting on Mouse Molecular Genetics, 1-5 September 2004.
Kiernan A.E., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D.M., Teasse C., Lovell-Badge R.H., Steel K.P. and Cheah K.S.E., Sox2 is required for sensory organ development in the mammalian inner ear, Nature. 2005, 434: 0131-1035.


Researcher : Lu L

List of Research Outputs

Huen M.S.Y., Li X.T., Lu L., Watt R.M., Liu D., Cheah K.S.E. and Huang J., Unraveling the molecular basis of the bacteriophage lambda-encoded recombination system in E. coli, Keystone Symposia , Keystone, Colorado, USA, 5-11 January 2005.
Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.
Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.


Researcher : Ma RYM

List of Research Outputs

Ma R.Y.M., Tong H.K., Cheung M.S., Tsang A.C.C., Leung G.W.Y. and Yao K.M., Journal of Cell Science, Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. 2004, 118: 000-000.
Ma R.Y.M., Tsang A.C.C., Leung G.W.Y. and Yao K.M., The mitogen-activated protein kinase pathway promotes mitotic progression through the forkhead box transcription factor foxmi, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.


Researcher : Mak ACY

List of Research Outputs

Mak A.C.Y. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family., Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D., Koopman P., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, Annual meeting of the international society for computational biology 2005. Michigan June 25-29 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D.A.G.M.A.R., Koopman P.E.T.E.R., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, ISMB Conerence 2005, 13th Annual International Conference on Intelligent Systems for Molecular Biology. Detroit Michigan, 25-29 June 2005.
Mak A.C.Y., Cheah K.S.E. and Smith D.K., Bioinformatics studies of gene regulation of SOX9 and the SOX family, 9th Research Postgraduate Symposium, HKU, 4 December 2004.


Researcher : Murphy G

List of Research Outputs

Chan W.Y., Cheah K.S.E., Ng V.C.W., Murphy G. and Chan D., Mouse model with impaired collagen X degradation at the chondro-osseous junction, Gordon Research Conference: Biology and Pathology of Cartilage. Il Ciocco, Barga, Italy June 2005.


Researcher : Ng AHL

List of Research Outputs

Huang J., Zhang X., Chen B., Ng A.H.L., Tanner J.A., Tay D.K.C., So K.F., Rachel R.A., Copeland N.G. and Jenkins N.A., Cre-transgenic mouse line for conditional gene knockout in retinal rod bipolar cells , INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 2005, 46: 3111S.
Ng A.H.L. and Huang J., Characterization of the specificity of Cre expression in cerebellar Purkinje cells, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Ng A.H.L., Zhang X. and Huang J., Characterize the specificity of CRE expression in cerebellar purkinje cells, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.


Researcher : Ng AYN

List of Research Outputs

Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D., Koopman P., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, Annual meeting of the international society for computational biology 2005. Michigan June 25-29 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D.A.G.M.A.R., Koopman P.E.T.E.R., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, ISMB Conerence 2005, 13th Annual International Conference on Intelligent Systems for Molecular Biology. Detroit Michigan, 25-29 June 2005.


Researcher : Ng DCH

List of Research Outputs

Leung T.H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Identification of multiple isoforms of DLC2, a novel tumor suppressor gene, and their functional characterization in hepatocellular carcinoma, 4th International Meeting of Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, December 2004 (Young Investigator Award). 2004.
Ng D.C.H., Jin D. and Ng I.O.L., Characterisation of Steroidogenic Acute Regulatory Protein Related Lipid Transfer Domain (START)domain in Deleted in Liver Cancer 2(DLC-2)., Society For Chinese Bioscientists in America, Beijing, July 2004 . 2004.
Ng D.C.H., Ching Y.P., Ng I.O.L. and Jin D., Functional characterization of a Novel RhoGAP protein deleted in liver cancer 2 (DLC2), 9th research postgraduate symposium, HKU, December 4, 2004.
Ng D.C.H., Ching Y.P., Chan S.F. and Jin D., Human T-cell leukemia virus type I oncoprotein tax targets the centrosome, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Ng VCW

List of Research Outputs

Chan W.Y., Cheah K.S.E., Ng V.C.W., Murphy G. and Chan D., Mouse model with impaired collagen X degradation at the chondro-osseous junction, Gordon Research Conference: Biology and Pathology of Cartilage. Il Ciocco, Barga, Italy June 2005.
Cheng Y.W., Chan W.C.W., Ng V.C.W., Cheung K.M.C. and Chan D., Molecular basis for increased bone formation in a mouse expressing mutant type X collagen , 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Wong Q.N.Y., Ng V.C.W., Lin M.C., Huang F.Y., Chan D. and Huang J., Efficient and seamless DNA recombineering using a thymidylate synthase a selection system in Escherichia coli, Nucleic Acids Research. 2005, 33(6): 2-9.


Researcher : Niewiadomska AK

List of Research Outputs

Cheah K.S.E., Niewiadomska A.K., Dung W.F., Lau J.Y.B., Hunziker E., Aszodi A., Gustafsson E., Yamada Y., Zhou Z., Tryggvason K. and Chan D., Perlecan compartmentalizes collagen X in the mammalian growth plate., XIXth meeting of Federation of the European Connective Tissue Societies: Taormina-Giardia Naxos, Italy July 2004.


Researcher : Ott J

List of Research Outputs

Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Sham P.C., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., The TRP2 allele of collagen IX as an age-dependent risk factor for the development and severity of intervertebral disc degeneration, Asia Pacific Orthopaedic Association 14th Triennial Congress, 5-10 September 2004.


Researcher : Pang WC

List of Research Outputs

Mak C.M., Pang W.C., Chan G.C.F., Wong W.K. and Tam S., Serial lipoprotein electrophoresis reveals the lipid changes in L-asparaginase-induced chylomicronaemia syndrome, British Journal of Biomedical Science. British, 2005, 62(2): 95-97.
Tan K.C.B., Tso A.W.K., Ma O.C., Pang W.C., Tam S. and Lam K.S.L., Determinants of postprandial triglyceride and remnant-like lipoproteins in type 2 diabetes, Diabetes Metabolism Research and Reviews. 2005, 21(2): 209-14.


Researcher : Pelling AL

List of Research Outputs

Cheah K.S.E., Kiernan A., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D...M. and Lovell-Badge R.H., Sox2 is requured for sensory organ development in the inner ear, Cold Spring Harbor Meeting on Mouse Molecular Genetics, 1-5 September 2004.
Kiernan A.E., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D.M., Teasse C., Lovell-Badge R.H., Steel K.P. and Cheah K.S.E., Sox2 is required for sensory organ development in the mammalian inner ear, Nature. 2005, 434: 0131-1035.
Lee Y.F., Pelling A.L., Leung K.K.H. and Cheah K.S.E., The molecular bases underlying the inner ear phenotypes of mouse mutants, yellow submarine (YSB)and light coat and circling (LCC), 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Pelling A.L., Kierman A., Leung K.K.H., Tang A.S.P., Bell D...M., Lovell-Badge R., Steel K. and Cheah K.S.E., Sox2 is required for sensory organ development in the inner ear, HGM2005 Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005 . 2004.


Researcher : Poon WH

List of Research Outputs

Poon W.H. and Wong N.S., The induction of p21/WAF1/CIP1 expression by the specific kappa opioid receptor agonist is independent of MAPK and PKC., 44th Annual Meeting Washington. DC December 4-8, 2004.


Researcher : Sae-Pang JJ

List of Research Outputs

Sae-Pang J.J., Zhang J. and Sham M.H., Analysis of multiple cardiac abnormalities of a knockout mouse mutant Hoxb3-lacZ, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Sae-Pang J.J., Zhang J. and Sham M.H., Analysis of multiple cardiac abnormalities of a knockout mouse mutant Hoxb3Hoxb3lacZ, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Sham M.H., Sae-Pang J.J., Wong E.Y.M., Mak S.S. and Ling K.W., The role of Hoxb3 in mammalian hindbrain patterning and craniofacial development, Anatomical Science international. 2004, 79s: 54.
Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interaction of Hoxb3 and Hoxb1 in hindbrain patterning revealed by ectopic expression of Hoxb3 in rhombomere 4 , 17 Annual Meeting on Mouse Molecular Genetics, Cold Spring Harbor Laboratory, New York, 1-5 September 2004.


Researcher : Sham MH

Project Title:Hoxb3lacZ: a mutant mouse model for studying the roles of Hoxb3 in heart and thoracic body wall development
Investigator(s):Sham MH
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:09/2003
Abstract:
To characterize the Hoxb3(lacZ) mutant locus and examine if this is a hypomorphic mutant; to analyse the cardiac abnormalities in the Hoxb3(lacZ) mutant using molecular markers and determine the role of Hoxb3 during heart development; to examine the ventral body wall defect by morphological and molecular markers, and identify any cooperation of Hoxb3 with other Hox genes in the developmental processes involved.


Project Title:Hoxb3lacZ: a mutant mouse model for studying the roles of Hoxb3 in heart and thoracic body wall development
Investigator(s):Sham MH
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2003
Abstract:
To characterize the Hoxb3(lacZ) mutant locus and examine if this is a hypomorphic mutant; to analyse the cardiac abnormalities in the Hoxb3(lacZ) mutant using molecular markers and determine the role of Hoxb3 during heart development; to examine the ventral body wall defect by morphological and molecular markers, and identify any cooperation of Hoxb3 with other Hox genes in the developmental processes involved.


Project Title:Mapping the gene for a novel blind mouse mutant
Investigator(s):Sham MH, Song Y
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Completion Date:01/2005
Abstract:
To identify the mutation that leads to the blind phenotype of the mutant mouse strain we produced by genome-wide mapping using microsatellite markers.


Project Title:Investigating the roles of Sox10 in the maintenance and differentiation of vagal neural crest cells during enteric nervous system development
Investigator(s):Sham MH
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2004
Abstract:
Refer to hard copy


List of Research Outputs

Chan K.K., Chen Y.S., Yau T.O., Fu M., Lui V.C.H., Tam P.K.H. and Sham M.H., Hoxb3 vagal neural crest-specific enhancer element for controlling enteric nervous system development, Developmental Dynamics. 2005, 233: 473-483.
Chan K.T. and Sham M.H., Disruption of the mouse Hoxb3 locus affects ventral body wall development, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Chan L.C., Kong C.T. and Sham M.H., The leukaemia fusion gene, Mll-Een affects haemopoietic development in mouse embryonic stem cells with enhanced proliferation and self renewal of myeloid progenitors, Days of Molecular Medicine 2005 – Stem Cell Biology and Human Disease, 17-19 March 2005, Salk Institute, La Jolla, California, USA. 2005.
Fu M., Lui V.C.H., Sham M.H., Pachnis V. and Tam P.K.H., Sonic hedgehog regulates the proliferation, differentiation, and migration of enteric neural crest cells in gut., The Journal of Cell Biology. 2004, 166(5): 673-684.
Garcia-Barcelo M.M., Ganster R.W., Lui V.C.H., Leon Y.Y., So M.T., Lau A.M.F., Fu M., Sham M.H., Knight J., Zannini M.S., Sham P.C. and Tam P.K.H., TTF-1 and RET promoter SNPs: regulation of RET transcription in Hirschsprung's disease, Human Molecular Genetics. 2005, 14(2): 191-204.
Law M.L., Tsang W.H., Chan K.K. and Sham M.H., Abnormal enteric ganglia development in a SOX 10 mouse mutant generated by gene targeting, 9th Rearch Postgraduate Symposium, HKU4 December 2004.
Law M.L. and Sham M.H., Abnormal enteric ganglia development in a Sox10 mouse mutant generated by gene targeting, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Law M.L., Ngan E.S.W., Lui V.C.H., Tam P.K.H. and Sham M.H., The role of Sox 10 on survival and proliferation of enteric neural crest stem cells, 3rd Annual Meeting of International Society for Stem Cell Research.. 2005.
Lu L., Zhang M., Cheung C.T., Wong C., Ko K.H., Tsang S.L., Chan L.C. and Sham M.H., Hoxb3 deficiency impairs B lymphopoiesis, 12th International Conference for Immunology, Montreal, Canada, July 18-23, 2004. 2004.
Lui V.C.H., Fu M., Sham M.H. and Tam P.K.H., Sonic hedgehog (SHH) promotes proliferation and maintains multi-potency of enteric neural crest stem cells (NCSCs), 51st Annual International Congress of British Association of Paediatric Surgeons, Oxford, U.K. 27-30 July 2004.
Lui V.C.H., Fu M., Sham M.H. and Tam P.K.H., Sonic hedgehog (SHH) regulates the migration and differentiation of the enteric neural crest stem cells (NCSCs) in gastrointestinal tract, 51st Annual International Congress of British Association of Paediatric Surgeons, Oxford, U.K. 27-30 July 2004.
Sae-Pang J.J., Zhang J. and Sham M.H., Analysis of multiple cardiac abnormalities of a knockout mouse mutant Hoxb3-lacZ, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Sae-Pang J.J., Zhang J. and Sham M.H., Analysis of multiple cardiac abnormalities of a knockout mouse mutant Hoxb3Hoxb3lacZ, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Sham M.H., Ectopic expression of Hoxb3 in the mouse hindbrain causes abnormal ear development, Centre for Developmental Biology, RIKEN Research Centre, Kobe, Japan. 2005.
Sham M.H., The role of Hoxb3 in mammalian hindbrain patterning and craniofacial development, 16th International Congress of the IFAA, Anatomical Science 2004 From Gene to Body, Kyoto, Japan. 2004.
Sham M.H., Sae-Pang J.J., Wong E.Y.M., Mak S.S. and Ling K.W., The role of Hoxb3 in mammalian hindbrain patterning and craniofacial development, Anatomical Science international. 2004, 79s: 54.
Shi J., Zheng D., Liu Y., Sham M.H., Tam P.K.H., Farzaneh F. and Xu R., Overexpression of soluble TRAIL induces apoptosis in human lung adenocarcinoma and inhibits growth of tumor xenografts in nude mice., Cancer Research. 2005, 65(5): 1687-1692.
Siu K.Y., Sham M.H. and Chow B.K.C., Secretin, a known gastrointestinal peptide, is widely expressed during mouse embryonic development, Gene Expression Patterns . 2005, 5: 445-451.
Wong E.Y.M. and Sham M.H., Ectopic expression of Hoxb3 in the hindbrain causes abnormal ear development, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005.
Wong E.Y.M., Cheah K.S.E. and Sham M.H., Ectopic expression of Hoxb3 in otic vesicle causes abnormal ear development, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interaction of Hoxb3 and Hoxb1 in hindbrain patterning revealed by ectopic expression of Hoxb3 in rhombomere 4 , 17 Annual Meeting on Mouse Molecular Genetics, Cold Spring Harbor Laboratory, New York, 1-5 September 2004.
Xu R., Li H., Cai K., Sham M.H., Tam P.K.H. and Lam K.S.L., Distribution, pathway and dynamics intake of recombinant adeno-associated virus and gene expression in the gut after oral administration, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004. P2-13-17.
Zhang M., Ko K.H., Sham M.H. and Lu L., Novel function of HOXB3 gene in regulation B lymphopoiesis , 9th research postgraduate symposium, HKU, December 4, 2004.


Researcher : Sham PC

Project Title:An association screen for schizophrenia susceptibility loci in a high-LD, gene-rich region of chromosome 3p
Investigator(s):Sham PC
Department:Psychiatry
Source(s) of Funding:Small Project Funding
Start Date:07/2004
Abstract:
To identify novel susceptibility loci for schizophrenia, by conducting a high-density SNP screen of a region recently identified by a meta-analysis of 20 genome-wide linkage scans.


List of Research Outputs

Garcia-Barcelo M.M., Ganster R.W., Lui V.C.H., Leon Y.Y., So M.T., Lau A.M.F., Fu M., Sham M.H., Knight J., Zannini M.S., Sham P.C. and Tam P.K.H., TTF-1 and RET promoter SNPs: regulation of RET transcription in Hirschsprung's disease, Human Molecular Genetics. 2005, 14(2): 191-204.
Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Sham P.C., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., The TRP2 allele of collagen IX as an age-dependent risk factor for the development and severity of intervertebral disc degeneration, Asia Pacific Orthopaedic Association 14th Triennial Congress, 5-10 September 2004.
Song Y., Cheung K.M.C., Karppinen J., Ho D.W.H., Yip S...P., Leong J...C...Y., Ott J., Luk K.D.K., Cheah K.S.E., Sham P.C. and Chan D., Association studies of intervertebral disc disease with genes in the aggrecan degradation pathway, HUGO 2005: Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005.


Researcher : Shum DKY

Project Title:The role of chondroitin 6-sulfotransferase in post-traumatic regeneration of peripheral nerves
Investigator(s):Shum DKY, Chung SK, Chan YS
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:10/2001
Completion Date:09/2004
Abstract:
To identify the cell type(s) that produce the CS moiety in question; to determine the time course and expression pattern of C6ST in relation to axon regrowth and conduction across the crush-injured nerve; to determine if suppression of C6ST changes the efficacy of regeneration of crushed nerves.


Project Title:Expression of heparanase in the injured spinal cord
Investigator(s):Shum DKY
Department:Biochemistry
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Abstract:
To determine the time course and cellular source of heparanase expression in the bridged hemicord; to determine the distribution of substrate HS in relation to the heparanase-expressing cells.


Project Title:Glycosaminoglycan-modulating strategies to promote plasticity and repair after spinal cord injury
Investigator(s):Shum DKY
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2004
Abstract:
Refer to hard copy


Project Title:Expression of chondroitin sulfotransferases in the injured spinal cord
Investigator(s):Shum DKY
Department:Biochemistry
Source(s) of Funding:Small Project Funding
Start Date:11/2004
Abstract:
To determine the expression profiles of the chondroitin sulfotransferases (STs) with time; to map the expression of chondroitin STs in reactive astrocytes, macrophages and meningeal fibroblasts that invade the leison site; to recover chondroitin sulfates of the lesion site and surrounding glial scar for comparison of sulfation patterns with those of intact tissue.


List of Research Outputs

Chan D., Yang L., Hui W.S., Chan W.C.W., Huang J., Shum D.K.Y., Cheung K.M.C., Luo Z.J. and Cheah K.S.E., A splicing mutation in the ext2 gene affects messenger RNA stability in a family with hereditary multiple exostoses, XIXth Meeting of the Federation of the European Connective Tissue Societies, Taormina-Giardini Noxos, Italy. 9-13 July 2004.
Chan Y.S., Lai C.H., Ng K.Y. and Shum D.K.Y., Development and compensation of the gravity sensing system: Implications on neural plasticity, In: X.M. Wang et al. (Eds) Progress in Neuroscience. Higher Education Press, Beijing. 2004, 39-55.
Chau C.H., Liu J., Chan Y.S. and Shum D.K.Y., Expression of chondroitin sulfotransferases in the injured spinal cord, Soc. Neuroscience Abstract (U.S.A.). 2004, 43.6.
Kwok J.C.F., Ng K.Y., Zhang F., Chan Y.S. and Shum D.K.Y., Chondroitin sulfates restrict axonal growth along the projection path of vestibular nuclear neurons across the hindbrain of prenatal rats, Neuroscience Letters. 2004, 370 Suppl: S13–14.
Lai C.H., Tse Y.C., Shum D.K.Y., Yung K.K. and Chan Y.S., Postnatal expression of Fos in otolith-related brainstem neurons of rats following off-vertical axis rotation, J. Comparative Neurology. 2004, 470: 282-296.
Li C., Zhang Y.K., Guan Z.L., Shum D.K.Y. and Chan Y.S., Vestibular afferent innervation in the efferent vestibular nucleus of rats, Neuroscience Letters. 2005, 385: 36-40.
Liu H. and Shum D.K.Y., Chondroitin sulfate proteoglycans (CSPG) and hyaluronan at borders between astrocytes and schwann cells restrict neurite crossing., Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Liu H. and Shum D.K.Y., Chondroitin sulfate proteoglycans (CSPG) produced by schwann cells in astrocyte-schwann cell cocultures limit neurite extension and crossing of cellular boundaries, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Yeung M.N. and Shum D.K.Y., Expression of heparanase and syndecan-3 are colocalized in mouse growth plate, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Zhang F., Lai C.H., Li J.L., Shum D.K.Y. and Chan Y.S., Expression of TrkA, TrkB and TrkC receptors in the central pathway of trigeminal proprioception in the rat, Acta Anatomica Sinica. 2004, 35: 249-252.
Zhang Y. and Shum D.K.Y., Heparanase colocalizes with syndecan-3 in neurons of normal adult spinal cord, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Zhang Y. and Shum D.K.Y., To understand the expression profile and function of heparanase in the adult spinal cord, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Siu KL

List of Research Outputs

Chan C.P., Siu K.L., Zheng B. and Jin D., Subcellular localization of sars coronavirus orf8 protein, 10th SCBA International Symposium, Beijing, China18-23 July 2004.
Siu K.L., Wong C.M. and Jin D., 10th SCBA International Symposium, Beijing, China, 18-23 July 2004, Peroxiredoxin-null yeast cells are genomically unstable. 2004.


Researcher : Siu STS

List of Research Outputs

Poon G.W.K., Mak C.M., Wong R.M.S., Wong K.Y., Yung T.C., Tam S., Siu S.T.S., Lee J.S.K. and Low L.C.K., Reversible Myocardial Damage in a Premature Infant with Carnitine-Acylcarnitine Translocase Deficiency, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 33.


Researcher : Smith DK

Project Title:Patterns in proteins with unusual flexibility profiles
Investigator(s):Smith DK
Department:Biochemistry
Source(s) of Funding:Seed Funding for New Staff
Start Date:08/2002
Abstract:
To identify what makes some proteins imcompatible with flexibility parameters calculated from a large set of protein structures.


Project Title:Distinguishing among functional, chance occurrences and variations in the DNA sequences bound by transcription factors
Investigator(s):Smith DK, Yao KM
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2003
Abstract:
To improve computationally based methods for the detection of transcription factor binding sites in genomic DNA and to distinguish whether slight variations in binding site preference among structurally related transcription factors represent functionally important differences.


List of Research Outputs

Cai J., Smith D.K., Xia X. and Yuen K.Y., MBEToolbox: a MATLAB toolbox for sequence data analysis in molecular biology and evolution., BMC bioinformatics. 2005, 6(1): 64.
Fu W., Chung B.H.Y., Smith D.K. and Cheung P.T., Automated method for the identification of conserved non-coding regions in hypoxic-ischaemic regulated genes in different species, The 3rd Hong Kong Medical Genetics Conference, HK< 8-10 April 2005. 15.
Fu W., Chung B.H.Y., Chan K.W., Bhatia I., Smith D.K. and Cheung P.T., Sequence analysis for putative transcription factor binding sites of a novel hypoxic-ischaemic regulated gene - HID-1, The 3rd Hong Kong Medical Genetics Conference, HK< 8-10 April 2005. 30.
He W., Gong K., Smith D.K. and Ip N. .Y., The N-Terminal cytokine binding domain of LIFR is required for CNTF binding and signaling, FEBS . Elsevier B.V., 2005, 579: 4317-4323.
Mak A.C.Y. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family., Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D., Koopman P., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, Annual meeting of the international society for computational biology 2005. Michigan June 25-29 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D.A.G.M.A.R., Koopman P.E.T.E.R., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, ISMB Conerence 2005, 13th Annual International Conference on Intelligent Systems for Molecular Biology. Detroit Michigan, 25-29 June 2005.
Mak A.C.Y., Cheah K.S.E. and Smith D.K., Bioinformatics studies of gene regulation of SOX9 and the SOX family, 9th Research Postgraduate Symposium, HKU, 4 December 2004.
Tan W.K. and Smith D.K., Patterns of houskeeping genes in cancer, 12th International Conference on Intelligent System for Molecular Biology and 3rd European Conference on Computational Biology at Glasgow, UK, July 31 - August 4, 2004.
Yap Y.L., Lam C.L., Girard L., Zhang X., Hernandez D., Gras R., Wang E., Chiu S. .W., Chung L.P., Lam W.K., Smith D.K., Minna J. .D., Danchin A. and Wong M.P., Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays, Nucleic Acids Research. Oxford University Press 2005, 2004, 33 No. 8: 2764.


Researcher : So CL

List of Research Outputs

Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., So C.L., Kwan K.M., Hunziker E.B., Yamada Y., Bateman J.F., Cheung K.M.C. and Chan D., Chondrocytes survive ER stress caused by unfolded proteins via re-programming, HGM2005 HUGO's 10th Human Genome Meeting, Kyoto, Japan 18-21 April 2005.


Researcher : Song Y

Project Title:Genetic linkage analysis of early onset degenerative disc disease in Southern Chinese
Investigator(s):Song Y, Cheah KSE, Cheung KMC, Leong JCY, Chan D
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:11/2003
Abstract:
To detect linkage associated with early onset familial DDD by performing a genome-wide scan; to define the chromosomal location of the early onset familial DDD gene; to begin preliminary work towards positional/candidate clonning.


Project Title:Genetic linkage analysis of early onset degenerative disc disease in Southern Chinese
Investigator(s):Song Y, Cheah KSE, Cheung KMC, Leong JCY, Chan D
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:11/2003
Abstract:
To detect linkage associated with early onset familial DDD by performing a genome-wide scan; to define the chromosomal location of the early onset familial DDD gene; to begin preliminary work towards positional/candidate clonning.


Project Title:Mapping and cloning a new gene on chromosome 8q24 for amyotrophic lateral sclerosis in a large Chinese family
Investigator(s):Song Y, Ho SL, Fong CY, Ramsden D.B.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:01/2005
Abstract:
To finely map the candidate region using recombinational and linkage disequilibrium approaches; to identify and prioritize candidate genes in this interval for mutation analysis using bioinformatics tools to assess function, Northern blot to assess expression in the nervous system and microarray/quantitative PCR methods to assess differences in candidate gene expression between affected and normal individuals; Screen pathogenic mutations are found, investigate the frequency of mutations in this gene in individuals with sporadic ALS to determine the risk for ALS attributable to this gene.


Project Title:Mapping and cloning a new gene on chromosome 8q24 for amyotrophic lateral sclerosis in a large Chinese family
Investigator(s):Song Y
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
Refer to hard copy


List of Research Outputs

Chu S.F., Tam C.M., Kam K.M., Song Y., Lau Y.L. and Chiang A.K.S., b-Chemokine Polymorphisms are not Associated with Tuberculosis in the Hong Kong Chinese Population, The 3rd Hong Kong Medical Genetics Conference, Hong Kong, 8-10 April 2005.
Jim J.J.T., Noponen-Hietala N., Cheung K.M.C., Ott J., Karppinen J., Sahraravand A., Luk K.D.K., Yip S.P., Sham P.C., Song Y., Leong J.C.Y., Cheah K.S.E., Ala-Kokko L. and Chan D., The TRP2 allele of collagen IX as an age-dependent risk factor for the development and severity of intervertebral disc degeneration, Asia Pacific Orthopaedic Association 14th Triennial Congress, 5-10 September 2004.
Song Y., Cheung K.M.C., Karppinen J., Ho D.W.H., Yip S...P., Leong J...C...Y., Ott J., Luk K.D.K., Cheah K.S.E., Sham P.C. and Chan D., Association studies of intervertebral disc disease with genes in the aggrecan degradation pathway, HUGO 2005: Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005.


Researcher : Tai CP

List of Research Outputs

Tai C.P., Wynn S.L. and Cheah K.S.E., In Vivo Analysis of Specificity of Target Gene Transactivation by SOX9 , The 4th Annual Australian Developmental Biology Workshop30th September - 4th October 2004 Rottnest Island, Perth, Western Australia . 2004.
Tai C.P. and Cheah K.S.E., In vivo analysis of the transactivation specificity of group E SOX proteins, 13th conference of the international society of differentiation Sheraton Waikiki, Honolulu, HawaiiSeptember 5-9, 2004. 2004.
Tai C.P. and Cheah K.S.E., In vivo analysis of the transactivation specificity of group E SOX proteins, 9th Research Postgraduate Symposium, HKU, 4 December 2004.
Tai C.P. and Cheah K.S.E., In vivo analysis of the transactivation specificity of group E SOX proteins, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Tam PPL

List of Research Outputs

Cheah K.S.E., Wong S.Y.Y., Zhang J.C.L., Leung W.L., Kung H. and Tam P.P.L., Genetic analysis of the role of procollagen IIA as an extracellular regulator of BMP signaling, Frontiers in Biomedical Research, HKU 2004, 3 December 2004 Hong Kong. 2004.


Researcher : Tam S

List of Research Outputs

Cheung Y.F., Karmin O., Tam S. and Siow Y.L., Induction of MCP1, CCR2 and iNOS Expression in THP-1 Macrophages by Serum of Children Late After Kawasaki Disease, 13th Annual Scientific Congress of Hong Kong College of Cardiology (abstract published in the Journal of the Hong Kong College of Cardiology), 22-24 April 2005 (Best paper award). 2005, 13: 51.
Chiu A., Tam S., Au W.Y., Chan S.C., Liu C.L. and Fan S.T., MARS treatment for a patient presenting with acquired hepatic glutamine synthetase deficiency after orthotopic liver transplantation (Case Report), Liver Transplantation. 2005, 11(3): 353-355.
Lam J.C., Ooi C.G.C., Tam S., Lam K.S.L. and Ip M.S.M., Insulin resistance and other cardiovascular risk factors in obstructive sleep apnea, American Thoracic Society International Conference, San Diego, USA. May 2005. Proceedings of the American Thoracic Society. 2005, p.A232.
Lam J.C., Lam K.S.L., Tam S., Lam C.L. and Ip M.S.M., The determinants of blood pressure in relation to obstructive sleep apnea, American Thoracic Society International Conference, San Diego, USA. May 2005. Proceedings of the American Thoracic Society. 2005, p.A878.
Lam J.C.M., Ooi C.G.C., Tam S., Khong P.L., Lam B. and Ip M.S.M., Obesity and dyslipidemia in Chinese patients with obstructive sleep apnea, 9th Congress of Asian Pacific Society of Respirology, Hong Kong, December 2004. Respirology. 2004, 9 (Supp): A143.
Lam J.C.M., Lam K.S.L., Tam S., Yan S.W. and Ip M.S.M., The determinants of blood pressure in relatin to obstructive sleep apnea, 10th Medical Research Conference, Department of Medicine, The University of Hong Kong, February 2005. Abstract Book: RM 58.
Lam J.C.M., Ooi C.G.C., Lam K.S.L., Tam S., Lai A.Y.K., Tsang K.W.T., Lam W.K. and Ip M.S.M., The relationship of insulin resistance, obesity and sleep-disordered breathing , 9th Congress of Asian Pacific Society of Respirology, Hong Kong, December 2004. Respirology . 2004, 9 (Supp): A143.
Lam J.C.M., Ooi C.G.C., Lam K.S.L., Tam S., Lai A.Y.K., Tsang K.W.T., Lam W.K. and Ip M.S.M., The relationship of insulin resistance, obesity and sleep-disordered breathing, 10th Medical Research Conference, Department of Medicine, The University of Hong Kong, February 2005. Abstract Book: RM 59.
Mak C.M., Pang W.C., Chan G.C.F., Wong W.K. and Tam S., Serial lipoprotein electrophoresis reveals the lipid changes in L-asparaginase-induced chylomicronaemia syndrome, British Journal of Biomedical Science. British, 2005, 62(2): 95-97.


Researcher : Tam SCF

List of Research Outputs

Cheung Y.F., Ho M.H.K., Tam S.C.F., Yung T.C. and Chau A.K.T., Elevated hs-CRP Levels and Increased Arterial Stiffness in Children with a History of Kawasaki Disease, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 26.
Cheung Y.F., Ho M.H.K., Tam S.C.F. and Yung T.C., Increased high sensitivity C reactive protein concentrations and increased arterial stiffness in children with a history of Kawasaki disease, Heart. 2004, 90: 1281-1285.
Lee P.P.W., Poon G.W.K., Kwan E.Y.W., Wong K.Y., Chan K.W., Lee J.S.K., Tam S.C.F., Lau E., Tang M.H.Y., Gu X.F., Low L.C.K. and Cheung P.T., Antenatal Diagnosis and Postnatal Management of Tetrahydrobiopterin-deficient Hyperphenyalaninemia in a Hong Kong Chinese Infant, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 37.


Researcher : Tam SMT

List of Research Outputs

Yeung M.L., Tam S.M.T., Tsang A.C.C. and Yao K.M., Spdzd2 is a putative regulatory factor of insulin gene expression and pancreatic b-cell growth, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Tan WK

List of Research Outputs

Tan W.K. and Smith D.K., Patterns of houskeeping genes in cancer, 12th International Conference on Intelligent System for Molecular Biology and 3rd European Conference on Computational Biology at Glasgow, UK, July 31 - August 4, 2004.


Researcher : Tang ASP

List of Research Outputs

Cheah K.S.E., Kiernan A., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D...M. and Lovell-Badge R.H., Sox2 is requured for sensory organ development in the inner ear, Cold Spring Harbor Meeting on Mouse Molecular Genetics, 1-5 September 2004.
Kiernan A.E., Pelling A.L., Leung K.K.H., Tang A.S.P., Bell D.M., Teasse C., Lovell-Badge R.H., Steel K.P. and Cheah K.S.E., Sox2 is required for sensory organ development in the mammalian inner ear, Nature. 2005, 434: 0131-1035.
Pelling A.L., Kierman A., Leung K.K.H., Tang A.S.P., Bell D...M., Lovell-Badge R., Steel K. and Cheah K.S.E., Sox2 is required for sensory organ development in the inner ear, HGM2005 Human Genome Meeting Programme and Abstract Book Kyoto, Japan 18th-21st April 2005 . 2004.


Researcher : Tanner JA

Project Title:Comparative Characterization of the Two Polyphosphate Kinases of M. tuberculosis
Investigator(s):Tanner JA
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2005
Abstract:
Inorganic polyphosphate (polyP) is present in every cell in nature and consists of chains of tens or hundreds of orthophosphate residues linked by high-energy phospoanhydride bonds. For decades, the molecule was ignored as a 'molecular fossil', but recent discoveries, principally by Nobel Prize winner Arthur Kornberg, have challenged this view and brought deserved attention to this forgotten biopolymer [1]. In prokaryotes, the molecule plays a critical role in physiological adjustments to growth, development, stress and deprivation [2], besides acting as a phosphate reservoir, a metal chelator, a buffer, and is an important factor in mRNA processing and degradadation. In eukaryotes, roles have only started to be uncovered in the last year or two, but the molecule has already been shown to be a regulatory factor in the proliferative signaling pathways of mammalian cells [3]. It is clear that this simple inorganic molecule plays fundamental and crucial roles that are only just beginning to be unravelled. Until 2002, it was believed that polyphosphates were synthesized by a single class of enzymes, the polyphosphate kinases (PPK). However, a landmark paper from Kornberg in 2002 challenged that view [4], and it was discovered that there are in fact two classes of polyphosphate synthetases in prokaryotes: PPK1 and PPK2. Some bacteria, such as E. coli and H. pylori, only possess PPK1, others, such as M. tuberculosis, have both PPK1 and PPK2, whilst a few others only have PPK2. As PPK1 and PPK2 only exist in microorganisms and are absolutely absent from higher eukaryotes, these are excellent targets for new classes of antibacterials. As a first step towards both new antibacterials against tuberculosis and to deepen our understanding of this fundamental class of enzymes, we here propose to purify and perform a comparative characterization of PPK1 and PPK2 from M. tuberculosis. We shall test the emerging hypothesis that PPK1 is primarily a Mg2+-dependent ATP driven kinase whilst PPK2 is primarily a Mn2+-dependent GTP driven kinase. If this hypothesis holds true, it carries significant insight into the relationship between inorganic polyphosphate and the cell cycle. We can break down the overall objectives of this seed project funding grant into a three-stage process: I. Cloning of the ppk1 and ppk2 genes from M. tuberculosis. II. Purification of the PPK1 and PPK2 proteins. III. Comparative enzymatic characterization of PPK1 and PPK2. 1. Kornberg, A., N. N. Rao, et al. (1999). "Inorganic polyphosphate: a molecule of many functions." Annu Rev Biochem 68: 89-125. 2. Kuroda, A., K. Nomura, et al. (2001). "Role of inorganic polyphosphate in promoting ribosomal protein degradation by the Lon protease in E. coli." Science 293(5530): 705-8. 3. Wang, L., C. D. Fraley, et al. (2003). "Inorganic polyphosphate stimulates mammalian TOR, a kinase involved in the proliferation of mammary cancer cells." Proc Natl Acad Sci U S A 100(20): 11249-54. 4. Zhang, H., K. Ishige, et al. (2002). "A polyphosphate kinase (PPK2) widely conserved in bacteria." Proc Natl Acad Sci U S A 99(26): 16678-83.


List of Research Outputs

Huang J., Zhang X., Chen B., Ng A.H.L., Tanner J.A., Tay D.K.C., So K.F., Rachel R.A., Copeland N.G. and Jenkins N.A., Cre-transgenic mouse line for conditional gene knockout in retinal rod bipolar cells , INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 2005, 46: 3111S.
Huang J., Tanner J.A. and Zhang X., METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES, US Patent WO2005010179. 2005.
Tanner J.A., Watt R.M., Kao R.Y.T. and Huang J., Targeting the SARS Coronavirus Helicase - Three Approaches to Inhibitor Development, FASEB Journal. 2005, 19: 217.9.
Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.
Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.


Researcher : Tong HK

List of Research Outputs

Ma R.Y.M., Tong H.K., Cheung M.S., Tsang A.C.C., Leung G.W.Y. and Yao K.M., Journal of Cell Science, Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. 2004, 118: 000-000.


Researcher : Tsang ACC

List of Research Outputs

Huang J., Leong M.K., Watt R.M., Tsang A.C.C., Danchin A.L.M., Cheah K.S.E. and Liu D., Recombinogenic TaggingReveals Dynamic Compartmentalization of the Escherichia Coli Proteome, 2nd International Conference on Pathways, Networks, and Systems: Theory and Experiments, October 15-20, 2004, Crete, Greece. 2004.
Ma R.Y.M., Tong H.K., Cheung M.S., Tsang A.C.C., Leung G.W.Y. and Yao K.M., Journal of Cell Science, Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. 2004, 118: 000-000.
Ma R.Y.M., Tsang A.C.C., Leung G.W.Y. and Yao K.M., The mitogen-activated protein kinase pathway promotes mitotic progression through the forkhead box transcription factor foxmi, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.
Yeung M.L., Tam S.M.T., Tsang A.C.C. and Yao K.M., Spdzd2 is a putative regulatory factor of insulin gene expression and pancreatic b-cell growth, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Tsang KY

List of Research Outputs

Cheah K.S.E., Tsang K.Y., Cheslett D., Chan W.C.W., So C.L., Kwan K.M., Hunziker E.B., Yamada Y., Bateman J.F., Cheung K.M.C. and Chan D., Chondrocytes survive ER stress caused by unfolded proteins via re-programming, HGM2005 HUGO's 10th Human Genome Meeting, Kyoto, Japan 18-21 April 2005.


Researcher : Tsang SL

List of Research Outputs

Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interaction of Hoxb3 and Hoxb1 in hindbrain patterning revealed by ectopic expression of Hoxb3 in rhombomere 4 , 17 Annual Meeting on Mouse Molecular Genetics, Cold Spring Harbor Laboratory, New York, 1-5 September 2004.


Researcher : Tsang WH

List of Research Outputs

Law M.L., Tsang W.H., Chan K.K. and Sham M.H., Abnormal enteric ganglia development in a SOX 10 mouse mutant generated by gene targeting, 9th Rearch Postgraduate Symposium, HKU4 December 2004.


Researcher : Wang X

List of Research Outputs

Wang X., Enget P.A.U.L. .C. and Lam V.M.S., "Structural" NADP+ and the dimer interface: Detailed analyses of G6PD class I deficient mutants at R393, 9th research postgraduate symposium, HKU, December 4, 2004.
Wang X., Engel P...C... and Lam V.M.S., "Structural" NAPP+and G6PD Class 1 deficient mutants, 5th Human Genome Organization (HUGO) Pacific Meeting and 6thAsia-Pacific Conference on Human Genetics. November 17,2004 Singapore. 2004.
Wang X., A study of Glucose-6-Phosphate dehydrogenase (G6PD) class I deficient mutants: R393G and R393H at the dimer interface versus other mutants, PhD Thesis. 2005.


Researcher : Watt RM

List of Research Outputs

Huang J., Leong M.K., Watt R.M., Tsang A.C.C., Danchin A.L.M., Cheah K.S.E. and Liu D., Recombinogenic TaggingReveals Dynamic Compartmentalization of the Escherichia Coli Proteome, 2nd International Conference on Pathways, Networks, and Systems: Theory and Experiments, October 15-20, 2004, Crete, Greece. 2004.
Huen M.S.Y., Li X.T., Lu L., Watt R.M., Liu D., Cheah K.S.E. and Huang J., Unraveling the molecular basis of the bacteriophage lambda-encoded recombination system in E. coli, Keystone Symposia , Keystone, Colorado, USA, 5-11 January 2005.
Sun H., Ge R., Watt R.M., He Q. and Huang J., Towards the understanding of nickel trafficking in helicobacter pylori: Expression and characterization of a his-rich protein, HPN, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.
Tanner J.A., Watt R.M., Kao R.Y.T. and Huang J., Targeting the SARS Coronavirus Helicase - Three Approaches to Inhibitor Development, FASEB Journal. 2005, 19: 217.9.
Tanner J.A., Zheng B., Zhou J., Watt R.M., Jiang J., Wong K.L., Lin Y., Lu L., He M.L., Kung H.F., Kesel A.J. and Huang J., The adamantane-derived bananins are potent inhibitors of the helicase activities and replication of SARS coronavirus, Chemistry & Biology. 2005, 12: 303-311.
Wu X.S., Xin L., Shang X.Y., Lu L., Watt R.M., Cheah K.S.E., Huang J., Liu D. and Liang C.C., Increased efficiency of oligonucleotide-mediated gene repair through slowing replication fork progression, Proceedings of the National Academy of Science. 2005, 102(7): 2508-2513.
Zheng B., Guan Y., He M.L., Sun H., Du L., Zheng Y., Wong K.L., Chen H., Chen Y., Lu L., Tanner J.A., Watt R.M., Niccolai N., Bernini A., Spiga O., Woo P.C.Y., Kung H.F., Yuen K.Y. and Huang J., Synthetic peptides outside the spike protein heptad repeat regions as potent inhibitors of SARS-associated coronavirus, Antiviral Therapy. 2005, 10: 393-403.


Researcher : Wong CM

List of Research Outputs

Siu K.L., Wong C.M. and Jin D., 10th SCBA International Symposium, Beijing, China, 18-23 July 2004, Peroxiredoxin-null yeast cells are genomically unstable. 2004.


Researcher : Wong EYM

List of Research Outputs

Sham M.H., Sae-Pang J.J., Wong E.Y.M., Mak S.S. and Ling K.W., The role of Hoxb3 in mammalian hindbrain patterning and craniofacial development, Anatomical Science international. 2004, 79s: 54.
Wong E.Y.M. and Sham M.H., Ectopic expression of Hoxb3 in the hindbrain causes abnormal ear development, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005.
Wong E.Y.M., Cheah K.S.E. and Sham M.H., Ectopic expression of Hoxb3 in otic vesicle causes abnormal ear development, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Wong E.Y.M., Sae-Pang J.J., Mak S.S., Ling K.W., Tsang S.L., Cheuk Y.C., Chan K.K., Cheah K.S.E. and Sham M.H., Interaction of Hoxb3 and Hoxb1 in hindbrain patterning revealed by ectopic expression of Hoxb3 in rhombomere 4 , 17 Annual Meeting on Mouse Molecular Genetics, Cold Spring Harbor Laboratory, New York, 1-5 September 2004.


Researcher : Wong IHN

Project Title:Epigenetic and genetic deregulation in pediatric solid tumors
Investigator(s):Wong IHN, Tam PKH
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:09/2003
Completion Date:03/2005
Abstract:
To study the transcriptional deregulation of SOCS1, SOCS2 and SOCS3 in pediatric solid tumors and cell lines derived from pediatric cancer patients in vivo; to investigate whether transcriptional repression of SOCS1, SOCS2 and SOCS3 is caused by epigenetic mechanisms, cancer cell lines will be treated with a demethylating drug and an inhibitor of histone deacetylase in order to reactivate their expression; to identify epigenetic and genetic alterations in SOCS1, SOCS2 and SOCS3 associated with the JAK/STAT and RAS signaling pathways in childhood neoplasia.


Project Title:Epigenetic and genetic deregulation in pediatric solid tumors
Investigator(s):Wong IHN, Tam PKH
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2003
Completion Date:03/2005
Abstract:
To study the transcriptional deregulation of SOCS1, SOCS2 and SOCS3 in pediatric solid tumors and cell lines derived from pediatric cancer patients in vivo; to investigate whether transcriptional repression of SOCS1, SOCS2 and SOCS3 is caused by epigenetic mechanisms, cancer cell lines will be treated with a demethylating drug and an inhibitor of histone deacetylase in order to reactivate their expression; to identify epigenetic and genetic alterations in SOCS1, SOCS2 and SOCS3 associated with the JAK/STAT and RAS signaling pathways in childhood neoplasia.


Project Title:Epigenetic deregulation of imprinted growth/apoptosis regulatory genes in the development of pediatric solid tumors
Investigator(s):Wong IHN, Tam PKH
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:03/2004
Completion Date:03/2005
Abstract:
To study the relationship between epigenetic alterations and transcriptional control of four critical imprinted growth/apoptosis regulatory genes (NOEY2, p73, WT1 and IPL) in pediatric solid tumors and pediatric cancer cell lines; to characterize these four critical imprinted growth/apoptosis regulatory genes with implications for pediatric carcinogenesis in vitro; to restore the normal patterns of regulation and expression of the four critical imprinted growth/apoptosis regulatory genes in pediatric cancer cell lines by demethylation treatment and inhibition of histone deacetylases.


Project Title:Epigenetic and genetic deregulation in association with RAS signaling in childhood neoplasia
Investigator(s):Wong IHN
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2004
Completion Date:03/2005
Abstract:
Refer to hard copy


List of Research Outputs

Wong I.H.N., Chan J.K.Y. and Tam P.K.H., Epigenetic deregulation of cell cycle progression: a novel therapeutic strategy for pediatric cancer, 38th Annual Meeting of the Pacific Association of Pediatric Surgery, Vancouver, Canada, 22-26 May 2005.


Researcher : Wong NS

Project Title:Kappa-opioid receptor mediated cellular stress response: the role of Inositol 1,4,5-trisphosphate and diacylglycerol
Investigator(s):Wong NS, Yao KM
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:10/2002
Abstract:
The Phospholipase-C (PLC)/inositol-lipid signaling mechanism is itself of widespread importance in cellular regulation and is coupled to all the three major subtypes of opioid receptors (OR), but how this signaling pathway mediates OR-simulated cellular responses is scarcely known. The objective of this project is to investigate the importance of the PLC/inositol-lipid pathway in KappaOr-induced stress responses.


Project Title:Identification of a novel signaling pathway that regulates the transcription of the stress-response gene GADD153
Investigator(s):Wong NS
Department:Biochemistry
Source(s) of Funding:Small Project Funding
Start Date:11/2003
Abstract:
To identity the intracellular signaling pathway that is responsible for mediating the effect of fenretinide/4HPR by elucidating the "4HPR-response element" in the proximal promoter of the GADD153 gene.


Project Title:The establishment of a model cellular system for the investigation of the regulation of mRNA-stability by endoplasmic retriculum stress in pancreactic [beta]-cell
Investigator(s):Wong NS
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:05/2005
Abstract:
refer hard copy proposal


List of Research Outputs

Lai W.L. and Wong N.S., ROS Mediates 4HPR-induced Post-transcriptional Expression Of Gadd153 Gene, Free Radical Biology and Medicine. Elsevier, 2005, 38(12): 1585.
Lai W.L. and Wong N.S., Reactive oxygen species (ROS) regulates the expression of the growth-arrest and DNA damage inducible gene-153 at the post-transcriptional level, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Lai W.L. and Wong N.S., Reactive oxygen species (ROS) regulates the expression of the growth-arrest-and DNA-damage inducible gene at the post-transcriptional level, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Poon W.H. and Wong N.S., The induction of p21/WAF1/CIP1 expression by the specific kappa opioid receptor agonist is independent of MAPK and PKC., 44th Annual Meeting Washington. DC December 4-8, 2004.
Zhao R., Wong N.S. and Tom W.M., Interferon-genhances nitric oxide synthase expression induced by paclitaxel in alveolar macrophages: The roles of ras/mek/erk and NF-kB in signal transduction, 9th research postgraduate symposium, HKU, December 4, 2004.
Zhao R., Wong N.S. and Tom W.M., Synergistically interferon-g enhances paclitaxel-induced nitric oxide production in a murine alveolar macrophage cell line: roles of NF-kB, MEK and PKCd, Free Radical Biology and Medicine. New York, Elsevier, 2004, 37: S102.


Researcher : Wong SYY

List of Research Outputs

Cheah K.S.E., Wong S.Y.Y., Zhang J.C.L., Leung W.L., Kung H. and Tam P.P.L., Genetic analysis of the role of procollagen IIA as an extracellular regulator of BMP signaling, Frontiers in Biomedical Research, HKU 2004, 3 December 2004 Hong Kong. 2004.


Researcher : Wynn SL

List of Research Outputs

Au Y.K., Wynn S.L., Cheah K.S.E. and Cheung K.M.C., Cre expression in the node and notochord in transgenic mice driven by FOXA2 minimal nothchord element, 51st Annual Meeting of the Orthopaedic Research Society, Washington DC, USA, February 20-23, 2005.
Au Y.K., Wynn S.L., Cheah K.S.E. and Cheung K.M.C., The use of a mouse model to understand the pathogenesis of campomelic dysplasia caused by the human mutation, Sox9Y440X, 24th Annual Congress of the Hong Kong Orthopaedic Association, Hong Kong, November 13-14, 2004.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D., Koopman P., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, Annual meeting of the international society for computational biology 2005. Michigan June 25-29 2005.
Mak A.C.Y., Ng A.Y.N., Wynn S.L., Wilhelm D.A.G.M.A.R., Koopman P.E.T.E.R., Cheah K.S.E. and Smith D.K., Bioinformatic studies of gene regulation involving SOX9 and the SOX family, ISMB Conerence 2005, 13th Annual International Conference on Intelligent Systems for Molecular Biology. Detroit Michigan, 25-29 June 2005.
Tai C.P., Wynn S.L. and Cheah K.S.E., In Vivo Analysis of Specificity of Target Gene Transactivation by SOX9 , The 4th Annual Australian Developmental Biology Workshop30th September - 4th October 2004 Rottnest Island, Perth, Western Australia . 2004.


Researcher : Yang L

List of Research Outputs

Chan D., Yang L., Hui W.S., Chan W.C.W., Huang J., Shum D.K.Y., Cheung K.M.C., Luo Z.J. and Cheah K.S.E., A splicing mutation in the ext2 gene affects messenger RNA stability in a family with hereditary multiple exostoses, XIXth Meeting of the Federation of the European Connective Tissue Societies, Taormina-Giardini Noxos, Italy. 9-13 July 2004.
Yang L. and Cheah K.S.E., Characterizing the re-activated Ihh singling in 13del programming chondrocytes, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Yang L., Chan D. and Cheah K.S.E., Genetic analyses of terminal differentiation of hypertrophic chondrocytes, 9th Research Postgraduate Symposium, HKU, December 4, 2004.


Researcher : Yao KM

Project Title:Investigating the role of a secreted protein sPIN-1 in the regulation of pancreatic [beta]-cell growth and differentiation
Investigator(s):Yao KM, Chung SK, Thomas M.K., Habener J.F.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2002
Abstract:
To study: (1) The cellular expression of xPIN-1 in pancreas, (2) The effect of purified sPIN-1 on growth and differentiation of primary and cultured islet cells in vitro; (3) The effect of over-expression sPIN-1 in pancreatic [beta]-cell in transgenic mice.


Project Title:Regulation of FOXM1 activity by the Ras/Raf/MAPK pathway
Investigator(s):Yao KM
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Completion Date:01/2005
Abstract:
To perform immunocytochemical, DNA binding and transcriptional analyses to investigate the molecular mechanism(s) underlying the enhancing effect of MEK1 on FOXM1 activity; to enrich our understanding of how the cell cycle is regulated by mitogenic signals, which is of major biological interest and crucial for devising strategies to enhance cell regeneration after tissue injury.


Project Title:Investigating the functional involvement of PDZD2 in the regulation of pancreatic beta cell gene expression and function
Investigator(s):Yao KM, Cheah KSE, Thomas M.K.
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2004
Abstract:
To investigate cellular model - inducible silencing of PDZD2 expression in INS-1E cells; to define PDZD2 function by pancreatic beta cell-specific gene inactivation in mice.


Project Title:Investigating the functional involvement of PDZD2 in the regulation of pancreatic beta cell gene expression and function
Investigator(s):Yao KM
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:01/2005
Abstract:
Refer to hard copy


List of Research Outputs

Ma R.Y.M., Tong H.K., Cheung M.S., Tsang A.C.C., Leung G.W.Y. and Yao K.M., Journal of Cell Science, Raf/MEK/MAPK signaling stimulates the nuclear translocation and transactivating activity of FOXM1c. 2004, 118: 000-000.
Ma R.Y.M., Tsang A.C.C., Leung G.W.Y. and Yao K.M., The mitogen-activated protein kinase pathway promotes mitotic progression through the forkhead box transcription factor foxmi, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.
Rawat R., Xu Z.F., Yao K.M. and Chye M.L., Identification of cis-elements in ethylene and circadian regulation of the Solanum melongena gene encoding cysteine proteinase, Plant Molecular Biology. Springer, 2005, 57: 629-643.
Stanojevic V., Yao K.M. and Thomas M.K., The coactivator bridge-1 increases transcriptional activation by pancreas duodenum bomeobox-1 (PDX-1), Molecular Cell Endocrinology. 2005, 15;237(1-2): 67-74.
Tam C.W., Yao K.M. and Shiu S.Y.W., Anti-proliferative effects of PDZD2 and its secreted protein sPDZD2 on prostate cancer cells, HKU 9th Research Postgraduate Symposium. 2004, 2004: 2.08.
Tam C.W., Yao K.M. and Shiu S.Y.W., Potential roles of PDZD2 in prostate cancer pathogenesis, Taiwan-Hong Kong International Symposium on Neural Physiology. 2004, 2004: 16.
Yeung M.L., Tam S.M.T., Tsang A.C.C. and Yao K.M., Spdzd2 is a putative regulatory factor of insulin gene expression and pancreatic b-cell growth, 10th SCBA International Symposium, Beijing, China18-23 July 2004.



Researcher : Yeung ML

List of Research Outputs

Yeung M.L., Tam S.M.T., Tsang A.C.C. and Yao K.M., Spdzd2 is a putative regulatory factor of insulin gene expression and pancreatic b-cell growth, 10th SCBA International Symposium, Beijing, China18-23 July 2004.


Researcher : Yeung MN

List of Research Outputs

Yeung M.N. and Shum D.K.Y., Expression of heparanase and syndecan-3 are colocalized in mouse growth plate, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Zhang X

List of Research Outputs

Huang J., Zhang X., Chen B., Ng A.H.L., Tanner J.A., Tay D.K.C., So K.F., Rachel R.A., Copeland N.G. and Jenkins N.A., Cre-transgenic mouse line for conditional gene knockout in retinal rod bipolar cells , INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE. 2005, 46: 3111S.
Huang J., Tanner J.A. and Zhang X., METHOD FOR CONSTRUCTING AND MODIFYING LARGE DNA MOLECULES, US Patent WO2005010179. 2005.
Ng A.H.L., Zhang X. and Huang J., Characterize the specificity of CRE expression in cerebellar purkinje cells, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.


Researcher : Zhang Y

List of Research Outputs

Zhang Y. and Shum D.K.Y., Heparanase colocalizes with syndecan-3 in neurons of normal adult spinal cord, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Zhang Y. and Shum D.K.Y., To understand the expression profile and function of heparanase in the adult spinal cord, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.


Researcher : Zhou H

List of Research Outputs

Chin K.T., Zhou H., Wong C.M., Lee M.F., Chan C.P., Qiang B.Q., Yuan J.G., Ng I.O.L. and Jin D., The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma, Nucleic Acids Research. 2005, 33(6): 1859-1873.


Researcher : Zhou Z

Project Title:Investigation of alternations in growth factor signaling and their contribution to defective intramembranous bone formation in mice lacking MT1-MMP
Investigator(s):Zhou Z
Department:Biochemistry
Source(s) of Funding:Merit Award for RGC CERG Funded Projects
Start Date:09/2003
Abstract:
To understand the Nature of defective calvarial osteogenesis and alterations in FGF signaling in MT1-MMP homozygous mutant mice; to regulate the MT1-MMP by FGF signaling.


Project Title:Investigation of alternations in growth factor signaling and their contribution to defective intramembranous bone formation in mice lacking MT1-MMP
Investigator(s):Zhou Z
Department:Biochemistry
Source(s) of Funding:Competitive Earmarked Research Grants (CERG)
Start Date:09/2003
Abstract:
To understand the Nature of defective calvarial osteogenesis and alterations in FGF signaling in MT1-MMP homozygous mutant mice; to regulate the MT1-MMP by FGF signaling.


Project Title:DNA double-strand break repair and accelerated aging in Zmpste24 deficient mice
Investigator(s):Zhou Z
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:02/2004
Completion Date:01/2005
Abstract:
To look into the double-strand DNA damage repair in Zmpste24 and lamin A mutant cells to understand whether unprocessed prelamin A and lack of lamin A (Knockout mice already got from Dr Stward, NIH) affects homologous recombination and nonhomologous end joining.


Project Title:Nuclear envelope integrity and the accelerated aging in Zmpste24 deficient mice
Investigator(s):Zhou Z
Department:Biochemistry
Source(s) of Funding:Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:07/2004
Abstract:
Refer to hard copy


Project Title:Investigating the mechanism underlying anti-cancer effect of farnesyl transferase inhibitors
Investigator(s):Zhou Z
Department:Biochemistry
Source(s) of Funding:Seed Funding Programme for Basic Research
Start Date:01/2005
Abstract:
refer hard copy proposal


List of Research Outputs

Chan J.K.M. and Zhou Z., MIT-MMP in FGF signaling, 9th Rearch Postgraduate Symposium, HKU 4 December 2004 . 2004.
Chan J.K.M. and Zhou Z., MT1-MMP in FGF Signaling, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Cheah K.S.E., Niewiadomska A.K., Dung W.F., Lau J.Y.B., Hunziker E., Aszodi A., Gustafsson E., Yamada Y., Zhou Z., Tryggvason K. and Chan D., Perlecan compartmentalizes collagen X in the mammalian growth plate., XIXth meeting of Federation of the European Connective Tissue Societies: Taormina-Giardia Naxos, Italy July 2004.
Liu B. and Zhou Z., Genomic instability caused by unprocessed prelamin A contributes to premature aging in mice lacking Zmpste24, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.
Liu B., Chan J.K.M., Huang J. and Zhou Z., Premature aging and genomic instability in mice lacking zmpste24, 9th Research Postgraduate Symposium, HKU, December 4, 2004 . 2004.
Mirastschijski U., Zhou Z., Rollman O., Tryggvason K. and Agren M.S., Wound healing in membrane-type-1 matrix metalloproteinase-deficient mice, Journal of Investigative Dermatology. 2004, 123(3): 600-2.
Oblander S.A., Zhou Z., Galvez B.G., Tryggvason K. and Apte S.S., Distinctive Functions Of Membrane Type 1 Matrix-metalloprotease (mt1-mmp Or Mmp-14) In Lung And Submandibular Gland Development Are Independent Of Its Role In Pro-mmp-2 Activation, Developmental Biology. 2005, 277: 255-269.
Zhou Z., Genomic Instability In Laminopathy Based Premature Aging, University of Cambridge, University of Cambridge, UK. 2005.
Zhou Z., Genomic Instability In Laminopathy Based Premature Aging, University of Cambridge, UK. 2005.
Zhou Z., Wang J., Cao R., Morita H., Soininen R., Chan J.K.M., Liu B., Cao Y. and Tryggvason K., Impaired angiogenesis, delayed wound healing and retarded tumor growth in perlecan heparan sulfate-deficient mice, Cancer Research. 2004, 15;64(14): 4699-702.


-- End of Listing --