Dept of Microbiology

DEPT OF MICROBIOLOGY

Researcher : Butt KM

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Researcher : Cai J

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

Researcher : Cao K

List of Research Outputs

Zheng B., Cao K., Chan C.P.Y., Duan Z.H., Gao Y., Wang M., Di B., Hollidt J.M., Bergmann A., Lehmann M., Renneberg I., Tam J.S.L., Chan P.K.S., Cautherley G.W.H., Fuchs D. and Renneberg R., Serum Neopterin for Early Assessment of Severity of Severe Acute Respiratory Syndrome, Clin Immunol. 2005, 16: 18-26.

Zheng Y., Cao K., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H., Lu L. and Zheng B., Complementary activation of peripheral natural killer cell immunity in nasopharyngeal carcinoma, Cancer Science. 2006, 97(9): 912-9.

Researcher : Chan CS

List of Research Outputs

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., Development of New Technique for Detection of HIV Specific Cytotoxic T Lymphocytes, Keystone Symposia HIV Vaccines (×6). 27 March-2 April. 2006.

Researcher : Chan HY

List of Research Outputs

Chu C.M., Cheng V.C.C., Hung I.F.N., Chan K.S., Tang S.F., Tsang T.H.F., Chan H.Y. and Yuen K.Y., Viral Load Distribution in SARS Outbreak, Emerging Infectious Diseases. 2005, 11(12): 1882-1886.

Researcher : Chan KCW

List of Research Outputs

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., Development of New Technique for Detection of HIV Specific Cytotoxic T Lymphocytes, Keystone Symposia HIV Vaccines (×6). 27 March-2 April. 2006.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Zhang H. and Lu L., Ex vivo expansion of functional CD4 T cells from HIV patients., The 6th International Workshop on HIV, Cells of Macrophage/Dendritic Lineage and Other Reservoirs.. 2005, 37.

Researcher : Chan KH

List of Research Outputs

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Chan K.H., Mak W.W., Ngan H.Y.S., Xu X., Screaton G., Tam P.K.H., Austyn J.M., Chan L.C., Yip S.P., Peiris J.S.M., Khoo U.S. and Lin C.L., Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection. , Nature Genetics. 2006, 38: 38-46.

Chiang A.K.S. and Chan K.H., Prospective studies on primary EBV infection in Chinese children, The Croucher Foundation Advanced Study Institute on Epstein-Barr Virus and Nasopharyngeal Carcinoma organized by The University of Hong Kong and The Hong Kong University of Science and Technology, Hong Kong, 23-27 January 2006.

Drosten C., Chan K.H. and Poon L.L.M., Viral diagnosis of SARS, In: Stohr K, Anderson L, Osterhaus A, Yuen KY, Peiris JSM (eds). Severe acute respiratory syndrome. Blackwell, Oxford, 2005, 64-71.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Lau S.K.P., To W.K., Tse P.W.T., Chan A.K.H., Woo P.C.Y., Tsoi H.W., Leung A.F.Y., Li K.S.M., Chan P.K.S., Lim W.W.L., Yung R.W.H., Chan K.H. and Yuen K.Y., Human parainfluenza virus 4 outbreak and the role of diagnostic tests, Journal of Clinical Microbiology. 2005, 43: 4515-4521.

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Lee P.P.W., Wong W.H.S., Leung G.M., Chiu S.S.S., Chan K.H., Peiris J.S.M., Lam T.H. and Lau Y.L., Risk-Stratified Seroprevalence of Severe Acute Respiratory Syndrome Coronavirus Among Children in Hong Kong, Pediatrics. 2006, 117(6): e1156-1162.

Lee P.P.W., Wong W.H.S., Leung G.M., Chiu S.S.S., Chan K.H., Peiris J.S.M., Lam T.H. and Lau Y.L., Risk-stratified seroperevalence of SARS-coronarvirus (SARS-CoV) among children in Hong Kong, Joint Annual Scientific Meeting, The Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association, 7 January 2006, Queen Elizabeth Hospital. 2006, 44.

Nicholls J.M., Butany J., Poon L.L.M., Chan K.H., Beh S.L., Poutanen S., Peiris J.S.M. and Wong M.P., Time course and cellular localization of SARS-CoV nucleoprotein and RNA in lungs from fatal cases of SARS, Public Library of Science Medicine . 2006, 3(2): e27.

Poon L.L.M., Wong W.Y., Chan K.H., Ng S.F., Yuen K.Y., Guan Y. and Peiris J.S.M., Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. , Journal of Clinical Microbiology. 2005, 43: 3457-3459.

Poon L.L.M., Leung C.S.W., Chan K.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates. , Journal of Medical Virology. 2005, 76: 435-40.

Poon L.L.M., Wong W.Y., Ma H.T., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S. and Peiris J.S.M., Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification, Clinical Chemistry. 2006, 52(2): 303-6.

Poon L.L.M., Chan K.H. and Drosten C., Viral diagnosis of SARS, Severe acute respiratory syndrome: a clinical guide.. Blackwell, 2005, 64-71.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Wong C.M., Yang L., Chan K.P., Leung G.M., Chan K.H., Guan Y., Lam T.H., Hedley A.J. and Peiris J.S.M., Influenza-associated hospitalization in a subtropical city, PLoS Medicine. 2006, 3(4): e121.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chen Z.W., Wong B.H.L., Chan J.K.H., Wong L.P., He W., Ma C., Chan K.H., Ho D.D. and Yuen K.Y., SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus, Vaccine. 2005, 23: 4959-4968.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Woo P.C.Y., Lau S.K.P., Huang Y., Tsoi H.W., Chan K.H. and Yuen K.Y., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia., Archives of virology. 2005, 150(11): 2299-311.

Researcher : Chan KM

List of Research Outputs

Lau S.K.P., Ng K.H.L., Woo P.C.Y., Yip K.T., Fung A.M.Y., Woo G.K.S., Chan K.M., Que T.L. and Yuen K.Y., Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures, Journal of Clinical Pathology. 2006, 59: 219-222.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M.Y., Tang S.F. and Yuen K.Y., Clostridium bacteremia characterized by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology. 2005, 58: 301-307.

Researcher : Chan MCW

Project Title:	Establishment of primary culture of human airway epithelial cells, as an in-vitro model to study human infection of coronavirus/avian influenza virus
Investigator(s):	Chan MCW, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004
Completion Date:	04/2006

Abstract:

To establish a primary culture of human airway and alveolar epithelial cells, which can act as an in-vitro model to study human infection of coronavirus and avian influenza virus.

Project Title:	Primary human lung epithelial cells as an in-vitro model to study the pathogenesis of SARS coronavirus infection
Investigator(s):	Chan MCW, Peiris JSM, Tsao GSW
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

The research objective is to define cytokine, chemokine, SARS functional receptor gene ACE2 and apoptotic related gene expression profile of human lung epithelial cells in response to infection with infectious and inactivated SARS coronavirus and purified viral proteins.

List of Research Outputs

Chan M.C.W. and Peiris J.S.M., Induction Of Proinflammatory Cytokines And Chemokines By Influenza A (H5N1) Viruses In Human Alveolar Epithelial Cells (typeII Pneumocytes), Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies 2005.

Chan M.C.W., Cheung C.Y., Chui W.H., Tsao G.S.W., Nicholls J.M., Chan Y.O., Chan W.Y., Long H.T., Poon L.L.M., Guan Y. and Peiris J.S.M., Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells, Respiratory Research. 2005, 6: 135.

Researcher : Chan WY

List of Research Outputs

Chan M.C.W., Cheung C.Y., Chui W.H., Tsao G.S.W., Nicholls J.M., Chan Y.O., Chan W.Y., Long H.T., Poon L.L.M., Guan Y. and Peiris J.S.M., Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells, Respiratory Research. 2005, 6: 135.

Researcher : Chan YO

List of Research Outputs

Chan M.C.W., Cheung C.Y., Chui W.H., Tsao G.S.W., Nicholls J.M., Chan Y.O., Chan W.Y., Long H.T., Poon L.L.M., Guan Y. and Peiris J.S.M., Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells, Respiratory Research. 2005, 6: 135.

Researcher : Chen H

Project Title:	Modification of cells for isolation and characterization of SARS CoV from animal sources
Investigator(s):	Chen H
Department:	Microbiology
Source(s) of Funding:	Seed Funding for New Staff
Start Date:	07/2004

Abstract:

To provide: (1) a panel of cell lines (pools) for the isolation of SARS CoV from animal sources; (2) information on molecular mechanism of interspecies transmission of SARS CoV; (3) clues for the further molecular virology studies on this virus.

Project Title:	Biological function/relevance for the 29 nucleotides insertion in the genome of animal SARS like CoV
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

This study will serve as a pilot investigating the potential effect of insertion/deletion on expression of viral proteins down stream of this region and provide clues for further investigation into the molecular mechanism for SARS coronavirus interspecies transmission.

Project Title:	Identification of the possible animal reservoirs for SARS-like coronavirus in southern China
Investigator(s):	Chen H, Guan Y, Fan X
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

Even though the SARS epidemic had been brought under control, the lack of understanding of the origin of SCoV make it impossible to determine wheter this disease might come back in future. The detection of SCoV-like virus in wild animal species in Guangdone raises the necessity of furhter understanding the epidemiology and ecology of SCoV or SCoV-like viruses. The objectives of this proposal 1) to identify the animal reservoirs of SCoV-like viruses; 2) to identify the susceptible species that could facilitate the SARS outbreak. 3) to establish a system for the detection and isolation of sCoV-like viruses. The findings generated by this project are of critical importance for scientific and public health points of view, and may provide clues how to prevent the re-emergence of SARS in humans.

Project Title:	Molecular basis of genesis and determinants for pathogenicity of avian influenza H5N1 virus genotype Z
Investigator(s):	Chen H
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2006

Abstract:

N/A

Project Title:	Molecular basis of genesis and determinants for pathogenicity of avian influenza H5N1 virus genotype Z
Investigator(s):	Chen H, Guan Y
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2006

Abstract:

To extend our current genetic and surveillance studies to investigate the molecular basis for the genesis of the dominant genotype Z and identify molecular determinants for the pathogenesis of H5N1 viruses, especially the potential for adaptation of H5N1 in chickens and mammals; to evaluate the replication efficiency (rate) of different H5N1 genotypes in mammalian cells using tissue culture; to analyze tissue transmission and pathogenesis of H5N1 isolates identified from 2000 to 2004 in Southern China in chickens and mice in a BL-3 facility; to investigate the relationship between pathogenicity and molecular differences using bioinformatics; to identify other possible molecular determinants of H5N1 virus pathogenesis in chicken and mice in addition to the recognized HA basic amino connecting peptide using reverse genetics techniques.

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chen H., Anti-Viral Drug Resistance in Avian H5N1 Viruses Circulating in Southeast Asia, The VIII International Symposium on Respiratory Viral Infections to be held March 16 -19, 2006 at Hawaii, USA. 2006.

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Chen H. and Guan Y., Market wild animals mediating SARS outbreak in Guangdong. , Forum on Tropical Infectious Diseases. Nanning, Guangxi, 2005.

Chen H. and Guan Y., Outbreak of H5N1 virus in water fowls in Qinghai lake, Who Avian Influenza Working Group. WHO avian influenza working group, Malta, 2005.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Guan Y. and Chen H., Resistance to anti-influenza agents., Lancet. 2005, 366(9492): 1139-40.

Hulse-Post D.J., Sturm-Ramirez K.M., Humberd J., Seiler P., Govorkova E.A., Krauss S., Scholtissek C., Puthavathana P., Buranathai C., Nguyen T.D., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Role of domestic ducks in the propagation and biological evolution of highly pathogenic H5N1 influenza viruses in Asia., Proceedings of the National Academy of Sciences of the United States of America. 2005, 102(30): 10682-7.

Sturm-Ramirez K.M., Hulse-Post D.J., Govorkova E.A., Humberd J., Seiler P., Puthavathana P., Buranathai C., Nguyen T.D., Chaisingh A., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Are ducks contributing to the endemicity of highly pathogenic H5N1 influenza virus in Asia?, Journal of Virology. 2005, 79(17): 11269-79.

Wang M., Yan M.Y., Xu H.F., Liang W.L., Kan B., Zheng B., Chen H., Zheng H., Xu Y.M., Zhang E.M., Wang H.X., Ye J.R., Li G.C., Li M.C., Cui Z.G., Liu Y.F., Guo R.T., Liu X.N., Zhan D.H., Zhou D.H., Zhao A.L., Hai R., Yu D.Z., Guan Y. and Xu J.G., SARS-CoV Infection In A Restaurant From Palm Civet., Emerg Infect Dis. 2005, 11: 1860-1865.

Webster R.G., Peiris J.S.M., Chen H. and Guan Y., H5N1 outbreaks and enzootic influenza, Emerging Infectious Diseases. 2006, 12(1): 3-8.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Researcher : Chen JHK

List of Research Outputs

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Researcher : Chen Y

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Zheng B., Duan Z.P., Zhao X., Huang D.Z., He L.X., Chen Y. and Zhao C.H., RANTES gene single nucleotide polymorphisms and expression in patients with chronic hepatitis B virus infection., International Meeting on the Molecular Biology of Hepatitis B Viruses. 18-21 September 2005. Heidelberg, Germany.. 2005.

Researcher : Chen Y

List of Research Outputs

Researcher : Cheng VCC

List of Research Outputs

Au W.Y., Kumana C.R., Lam C.W., Cheng V.C.C., Shek T.W.H., Chan E.Y.T., Liu K.Y. and Kwong Y.L., Solid tumors subsequent to arsenic trioxide treatment for acute promyelocytic leukemia, Leukemia Research. 2006.

Au W.Y., Cheng V.C.C. and Lie A.K.W., Toxoplasma Gondii Serology and Stem Cell Transplantation in Chinese, Transplantation. 2005, 37(10): 4497-4499.

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Cheng V.C.C., Yew W.W. and Yuen K.Y., Molecular diagnostics in tuberculosis., European journal of clinical microbiology & infectious diseases. 2005, 24(11): 711-720.

Cheng V.C.C., Ho P.L. and Yuen K.Y., Two probable cases of serious drug interaction between clarithromycin and colchicine., The Southern medical journal. 2005, 98(8): 811-3.

Cheng V.C.C., Ho P.L. and Yuen K.Y., Two probable cases of serious drug interaction between clarithromycin and colchicine, South Medical Journal. 2005, 98(8): 811-3.

Chu C.M., Cheng V.C.C., Hung I.F.N., Chan K.S., Tang S.F., Tsang T.H.F., Chan H.Y. and Yuen K.Y., Viral Load Distribution in SARS Outbreak, Emerging Infectious Diseases. 2005, 11(12): 1882-1886.

Hon C., Au W.Y. and Cheng V.C.C., Ophthalmic zoster sine herpete presenting as oculomotor palsy after marrow transplantation for acute myeloid leukemia, Haematologica. 2005, 90(12Suppl): EIM04.

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Researcher : Cheung CL

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Researcher : Cheung CY

List of Research Outputs

Chan M.C.W., Cheung C.Y., Chui W.H., Tsao G.S.W., Nicholls J.M., Chan Y.O., Chan W.Y., Long H.T., Poon L.L.M., Guan Y. and Peiris J.S.M., Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells, Respiratory Research. 2005, 6: 135.

Kien F.S., Kam Y.W., Roberts A., Cheung C.Y., Lamirande E. .W., Vogel L., Chu S.L., Chan C.M., Tse K.S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Immunization with trimeric S glycoprotein protects from SARS-CoV challenge despite the presence of in vitro facilitating antibodies, Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies 2005 - XIII International Congress of Virology . 2005.

Lee D.C.W., Mok K.P., Cheung C.Y., Law H.Y., Peiris J.S.M. and Lau A.S.Y., Mechanisms of H5N1 Avian Influenza Virus Induced TNF-alpha dysregulation and a Delay in Apoptosis in Primary Blood Macrophages, Cell Research (The 2005 Annual Meeting of International Society for Interferon & Cytokine Research), Shanghai, PR China, 20-24 October 2005. 15(10) Suppl: 100.

Lee D.C.W., Mok K.P., Cheung C.Y., Law H.Y., Peiris J.S.M. and Lau A.S.Y., The Paradox of H5N1 Avian Influenza Virus Infection in Primary Blood macrophages: TNF-alpha Hyper-induction and a Delay in Apoptosis. * Recipient of 2nd Prize Young Investigator Award., In: Davy C.W. Lee, Chris K.P. Mok, Chung-Yan Cheung, Anna H.Y. Law, Malik Peiris and Allan S.Y. Lau., 7th World Congress on Inflammation, 20 - 24 August 2005 in Melbourne, Australia.. 2005.

Lee D.C.W., Mok K.P., Cheung C.Y., Law H.Y., Peiris J.S.M. and Lau A.S.Y., The Paradox of H5N1 Avian Influenza Virus Infection in Primary Blood macrophages: TNF-alpha Hyper-induction and a Delay in Apoptosis, Inflammation Research (Official Journal of The International Societies, The European Histamine Research Society), 23 August 2005. 54: S181.

Lee D.C.W., Cheung C.Y., Law H.Y., Mok K.P., Peiris J.S.M. and Lau A.S.Y., p38 Mitogen-Activated Protein Kinase-Dependent Hyperinduction of Tumor Necrosis Factor Alpha Expression in Reponse to Avian Influenza Virus H5N1, Journal of Virology. 2005, 79(16): 10147-10154.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Differential expression of chemokines and their receptors in adult and neonatal macrophages infected with human or avian influenza viruses, 6^th Guangdong - Hong Kong Paediatric Exchange Meeting, Guangdong, PRC, 25 June 2006 (2^nd Prize in Poster Presentation). 2006, 38.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Functional Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Production by Avian Influenza Virus-Infected Macrophages, The Journal of Infectious Diseases. 2006, 193(7): 945-953.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Impairment of T lymphocytes activation in avian influenza: an in vitro study, 6th Guangdong – Hong Kong Paediatric Exchnage Meeting. China, 2006, 39.

Researcher : Ching PTY

List of Research Outputs

Ho P.L., Cheng J.C., Ching P.T.Y., Kwan J.K., Lim W.W.L., Tong W.C., Wu T.C., Tse C.W., Lam R., Yung R.W.H. and Seto W.H., Optimising antimicrobial prescription in hospitals by introducing an antimicrobial stewardship programme in Hong Kong: consensus statement, Hong Kong Medical Journal. 2006, 12(2): 141-8.

Researcher : Chong TK

Project Title:	Construction and characterization of delta AFMP1 delta AFMP2 deletion mutant of Aspergillus fumigatus
Investigator(s):	Chong TK, Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

In this project, we propose to study the essentiality and possible roles of AFMP1 and AFMP2 in Aspergillus fumigatus by constructing the deletion mutants, delta AFMP1, delta AFMP2 and delta AFMP1 delta AFMP2 of Aspergillus fumigatus. The neighborhoods of these genes in the genomes of A. fumigatus, A. nidulans and P. marneffei will also be explored.

List of Research Outputs

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

Woo P.C.Y., Lau S.K.P., Lau C.Y., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient, Bone Marrow Transplantation. 2005, 35: 831-833.

Researcher : Chow KH

List of Research Outputs

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

Ho P.L., Mak C.K., Tse C.W., Chow K.H. and Cheung C.H., Invasive Haemophilus influenzae isolates with decreased levofloxacin susceptibility in Hong Kong, Journal of Antimicrobial Chemotherapy. 2006, 57(2): 366.

Researcher : Dhanasekaran V

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Du L

List of Research Outputs

Kan B., Wang M., Jing H., Xu H., Jiang X., Yan M., Liang W., Zheng H., Wan K., Liu Q., Cui B., Xu Y., Zhang E., Wang H., Ye J., Li G., Li M., Cui Z., Qi X., Chen K., Du L., Gao K., Zhao Y.T., Zou X.Z., Feng Y.J., Gao Y.F., Hai R., Yu D., Guan Y. and Xu J., Molecular evolution analysis and geographic investigation of severe acute respiratory syndrome coronavirus-like virus in palm civets at an animal market and on farms., Journal of Virology. 2005, 79(18): 11892-900.

Researcher : Duan L

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Duan RS

List of Research Outputs

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

Researcher : Fan X

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Guan Y

Project Title:	Vaccines for the control of H5N1 and other avian influenza viruses
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	The University of Hong Kong Foundation Seed Grant
Start Date:	04/2002

Abstract:

To study vaccines for the control of H5N1 and other avian influenza viruses.

Project Title:	Genetic determinants of influenza virus for inter-species transmission: studies using a reverse genetic approach
Investigator(s):	Guan Y, Peiris JSM, Poon LLM, Shortridge KF
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	08/2002

Abstract:

Avian influenza viruses can directly impact on human and animal health and thus cause economic loss. The study aims to establishing a reverse genetic system and to determine the biological significance of the deletion of NA stalk and the NS genes, particularly in relation to the host-restriction and pathogenesis. Results can enhance understanding of the machanism of inter-species transmission of influenza virus.

Project Title:	Searching for the precursor of SARS-Cov in Southern China
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To find source of SARS-Cov, and how this virus emerged in humans.

Project Title:	The role of migratory birds in the spread of H5N1 influenza virus in Southern China
Investigator(s):	Guan Y
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2005
Completion Date:	06/2006

Abstract:

N/A

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Chan M.C.W., Cheung C.Y., Chui W.H., Tsao G.S.W., Nicholls J.M., Chan Y.O., Chan W.Y., Long H.T., Poon L.L.M., Guan Y. and Peiris J.S.M., Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells, Respiratory Research. 2005, 6: 135.

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Chen H. and Guan Y., Market wild animals mediating SARS outbreak in Guangdong. , Forum on Tropical Infectious Diseases. Nanning, Guangxi, 2005.

Chen H. and Guan Y., Outbreak of H5N1 virus in water fowls in Qinghai lake, Who Avian Influenza Working Group. WHO avian influenza working group, Malta, 2005.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Choi Y.K., Nguyen T.D., Ozaki H., Webby R.J., Puthavathana P., Buranathal C., Chaisingh A., Auewarakul P., Hanh N.T., Ma S.K., Hui P.Y., Guan Y., Peiris J.S.M. and Webster R.G., Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004., Journal of Virology. 2005, 79(16): 10821-5.

Guan Y., Antigenic shift and drift - evolution of avian influenza viruses, The Lancet Asia Medical Forum 2006, 3 - 4 May 2006, SUNTEC Singapore. 2006.

Guan Y., Establishment of multiple H5N1 virus sub-lineages in southeast Asia: implications for pandemic control, International Symposium on Recent Advances in Molecular, Clinical and Social Medicine organised by The University of Manitoba and the Shantou University in Shantou, China . Shantou, China, 2005.

Guan Y., H5N1 Influenza Endemic or Pandemic? Evidence from the field, American Society for Microbiology the 106th General Meeting in Orlando, FL, May 23-25, 2006.

Guan Y. and Chen H., Resistance to anti-influenza agents., Lancet. 2005, 366(9492): 1139-40.

Guan Y., The role of migratory birds in spread of H5N1 avian influenza virus over long distance , WHO Working Group on Influenza Research at the Human/Animal Interface organised by WHO in Malta. Malta, 2005.

Guan Y., 第一屆中國中西醫結合傳染病學術會議暨中國中西醫結合學會傳染病專業委員會成立大會, 第一屆中國中西醫結合傳染病學術會議暨中國中西醫結合學會傳染病專業委員會成立大會, 2006.

Hulse-Post D.J., Sturm-Ramirez K.M., Humberd J., Seiler P., Govorkova E.A., Krauss S., Scholtissek C., Puthavathana P., Buranathai C., Nguyen T.D., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Role of domestic ducks in the propagation and biological evolution of highly pathogenic H5N1 influenza viruses in Asia., Proceedings of the National Academy of Sciences of the United States of America. 2005, 102(30): 10682-7.

Kan B., Wang M., Jing H., Xu H., Jiang X., Yan M., Liang W., Zheng H., Wan K., Liu Q., Cui B., Xu Y., Zhang E., Wang H., Ye J., Li G., Li M., Cui Z., Qi X., Chen K., Du L., Gao K., Zhao Y.T., Zou X.Z., Feng Y.J., Gao Y.F., Hai R., Yu D., Guan Y. and Xu J., Molecular evolution analysis and geographic investigation of severe acute respiratory syndrome coronavirus-like virus in palm civets at an animal market and on farms., Journal of Virology. 2005, 79(18): 11892-900.

Poon L.L.M., Wong W.Y., Chan K.H., Ng S.F., Yuen K.Y., Guan Y. and Peiris J.S.M., Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. , Journal of Clinical Microbiology. 2005, 43: 3457-3459.

Poon L.L.M., Leung C.S.W., Chan K.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates. , Journal of Medical Virology. 2005, 76: 435-40.

Poon L.L.M., Wong W.Y., Ma H.T., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S. and Peiris J.S.M., Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification, Clinical Chemistry. 2006, 52(2): 303-6.

Shinya K., Hatta M., Yamada S., Takada A., Watanabe S., Halfmann P., Horimoto T., Neumann G., Kim J.H., Lim W.W.L., Guan Y. and Peiris J.S.M., Characterization of a human H5N1 influenza A virus isolated in 2003., Journal of Virology. 2005, 79(15): 9926-32.

Sturm-Ramirez K.M., Hulse-Post D.J., Govorkova E.A., Humberd J., Seiler P., Puthavathana P., Buranathai C., Nguyen T.D., Chaisingh A., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Are ducks contributing to the endemicity of highly pathogenic H5N1 influenza virus in Asia?, Journal of Virology. 2005, 79(17): 11269-79.

Wang M., Yan M.Y., Xu H.F., Liang W.L., Kan B., Zheng B., Chen H., Zheng H., Xu Y.M., Zhang E.M., Wang H.X., Ye J.R., Li G.C., Li M.C., Cui Z.G., Liu Y.F., Guo R.T., Liu X.N., Zhan D.H., Zhou D.H., Zhao A.L., Hai R., Yu D.Z., Guan Y. and Xu J.G., SARS-CoV Infection In A Restaurant From Palm Civet., Emerg Infect Dis. 2005, 11: 1860-1865.

Webster R.G., Peiris J.S.M., Chen H. and Guan Y., H5N1 outbreaks and enzootic influenza, Emerging Infectious Diseases. 2006, 12(1): 3-8.

Webster R.G., Guan Y., Poon L.L.M., Krauss S., Webby R., Govorkovai E. and Peiris J.S.M., The spread of the H5N1 bird flu epidemic in Asia in 2004, Archives of Virology Supplementum. 2005, 19: 117-29.

Wong C.M., Yang L., Chan K.P., Leung G.M., Chan K.H., Guan Y., Lam T.H., Hedley A.J. and Peiris J.S.M., Influenza-associated hospitalization in a subtropical city, PLoS Medicine. 2006, 3(4): e121.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

de Jong M.D., Thanh T.T., Khanh T.T., Hien V.M., Smith G.J., Chau N.V., Cam B.V., Qui P.V., Ha D.Q., Guan Y., Peiris J.S.M., Hien T.T. and Farrar J., Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection, New England Journal of Medicine. 2005, 353: 2667-2672.

Researcher : Guan Y

List of Research Outputs

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H. and Guan Y., Market wild animals mediating SARS outbreak in Guangdong. , Forum on Tropical Infectious Diseases. Nanning, Guangxi, 2005.

Chen H. and Guan Y., Outbreak of H5N1 virus in water fowls in Qinghai lake, Who Avian Influenza Working Group. WHO avian influenza working group, Malta, 2005.

Guan Y., Antigenic shift and drift - evolution of avian influenza viruses, The Lancet Asia Medical Forum 2006, 3 - 4 May 2006, SUNTEC Singapore. 2006.

Guan Y., H5N1 Influenza Endemic or Pandemic? Evidence from the field, American Society for Microbiology the 106th General Meeting in Orlando, FL, May 23-25, 2006.

Guan Y. and Chen H., Resistance to anti-influenza agents., Lancet. 2005, 366(9492): 1139-40.

Hulse-Post D.J., Sturm-Ramirez K.M., Humberd J., Seiler P., Govorkova E.A., Krauss S., Scholtissek C., Puthavathana P., Buranathai C., Nguyen T.D., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Role of domestic ducks in the propagation and biological evolution of highly pathogenic H5N1 influenza viruses in Asia., Proceedings of the National Academy of Sciences of the United States of America. 2005, 102(30): 10682-7.

Webster R.G., Peiris J.S.M., Chen H. and Guan Y., H5N1 outbreaks and enzootic influenza, Emerging Infectious Diseases. 2006, 12(1): 3-8.

Researcher : Guo C

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Ho PL

Project Title:	A phenotypic and genotypic analysis of vancomycin tolerance in Streptococcus pnemoniae
Investigator(s):	Ho PL, Yuen KY, Que TL
Department:	Microbiology
Source(s) of Funding:	Low Budget High Impact Programme
Start Date:	11/2000

Abstract:

To identify the frequency of occurrence of vancomycin tolerance in Streptococcus pneumoniae; to identify novel mutations in the histdine kinase (vncS) of tolerant pneumococci.

Project Title:	Molecular epidemiology of fluoroquinolone-resistant streptococcus pneumoniae in Hong kong and Canada
Investigator(s):	Ho PL, Low D.E.
Department:	Microbiology
Source(s) of Funding:	Low Budget High Impact Programme
Start Date:	11/2001

Abstract:

To define the molecular epidemiology of fluoroquinolone-resistant strains of S. pneumoniae isolated in Hong Kong and Canada.

Project Title:	Clinical outcome and impact on health care utilization of invasive pneumococcal disease in the era of antimicrobial resistance
Investigator(s):	Ho PL, Cheng YH, Chiu SSS, Tsang KWT, Yung R.W.H., Que T.L., Tsang D.N.C., Ng T.K.
Department:	Microbiology
Source(s) of Funding:	Health Care and Promotion Fund - Research Grant Projects
Start Date:	08/2002

Abstract:

To determine the impact of antibiotic resistance on patient outcome in invasive pneumococcal disease and to what extent additional health care resources are utilized as a result of the antibiotic resistance in a cohort of patients between 1997 to 2000; to document the serotypes of S. pneumoniae that casue invasive infection in the same cohort of patients.

Project Title:	Risk factors for SARS in health care workers following incubation of SARS patients
Investigator(s):	Ho PL, Hung CT, Yap F., Law K.I., Ho E.
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To identify factors associated with SARS transmission in HCW who participated in intubation of SARS patients; to define the transmission probability of an intubation procedure of SARS patient and to describe the intubation procedural practice of clinicians in the first two months of the outbreak.

Project Title:	Fluoroquinolone susceptibility clinical breakpoints and fluoroquinolone-resistant determining mutations among clinical strains of streptococcus pneumoniae
Investigator(s):	Ho PL, Wong SSY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To determine the frequency of occurrence of first step fluoroquinolone-resistant determining mutations among isolates categorized as susceptible by the currently accepted clinical breakpoints.

Project Title:	Molecular epidemiology of enterobacteriaceae producing extended-spectrum beta-lactamases in Hong Kong
Investigator(s):	Ho PL
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2005
Completion Date:	06/2006

Abstract:

N/A

Project Title:	Antimicrobial resistance among uropathogens causing well-defined episodes of uncomplicated cystitis among adult women in primary care settings
Investigator(s):	Ho PL, Yuen KY, Lam RMK, Kam KM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

(1) to assess prevalence of antimicrobial resistnace among uropathogens causing well-defined episodes of acute uncomplicated cystitis in adult women; (2) to assess the clonality, resistant determinants and virulence factor profiles of antibiotic-resistant strains of uropathogenic E. coli.

Project Title:	Evaluation of molecular tests for the rapid detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis and their impact on patient management
Investigator(s):	Ho PL, Yam WC, Leung CC, Yew WW, Mok YW, Chan KS, Tam CM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	04/2006

Abstract:

(1) to evaluate rapid tests for genotypic detection of rifampicin and isoniazid resistance in Mycobacterium tuberculosis in respiratory specimens and culture isolates; (2) to assess the effects of a model for targeted testing of patients with risks for multidrug-resistant tuberculosis on patient management

List of Research Outputs

Cheng V.C.C., Ho P.L. and Yuen K.Y., Two probable cases of serious drug interaction between clarithromycin and colchicine., The Southern medical journal. 2005, 98(8): 811-3.

Cheng V.C.C., Ho P.L. and Yuen K.Y., Two probable cases of serious drug interaction between clarithromycin and colchicine, South Medical Journal. 2005, 98(8): 811-3.

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

Ho P.L., Chau P.H., Yip P.S.F., Ooi C.G.C., Khong P.L., Ho J.C.M., Wong P.C., Ko C.F., Yan C.P.K. and Tsang K.W.T., A prediction rule for clinical diagnosis of severe acute respiratory syndrome, European Respiratory Journal. 2005, 26(3): 474-9.

Ho P.L., Antimicrobial resistance in Haemophilus species. M. Phil thesis submitted by Mr. Mak Chun Kit Gannon. Aug 2005, 2005.

Ho P.L., Antimicrobial stewardship program - Hong Kong experience, 2nd International Infection Control Conference. Hong Kong. 2006.

Ho P.L., Que T.L., Ng T.K., Chiu S.S.S., Yung R.W.H. and Tsang K.W.T., Clinical outcomes of bacteremic pneumococcal infections in an area with high resistance, European Journal of Clinical Microbiology and Infectious Diseases. 2006, 25(5): 323-7.

Ho P.L., Community-associated MRSA, 2nd International Infection Control Conference. Hong Kong.. 2006.

Ho P.L., Detection and characterization of extended-spectrum beta-lactamases among blood isolates of Providencia stuartii in Hong Kong. M.Med.Sc thesis submitted by Mr. Choy Wai Kit. Aug. 2005. , 2005.

Ho P.L., ESBL in the community and impact on the treatment of community-acquired infections, The 10th Hong Kong Medical Forum. The University of Hong Kong. Hong Kong.. 2005.

Ho P.L., Extended-spectrum beta-lactamase-producing Enterobacteriaceae, Antimicrobial drug resistance in Taiwan-Update. Taipei. Taiwan.. 台灣感染症醫學會, 中華民國微生物學會, 2005.

Ho P.L., Extended-spectrum beta-lactamases in fecal isolates of Escherichia coli from human and food animals. M. Phil thesis submitted by Mr. Duan Rong Shuai. Aug 2005., 2005.

Ho P.L., Extended-spectrum beta-lactamases in the community and impact on treatment of community-acquired infections, 10th Hong Kong Medical Forum. Department of Medicine, The University of Hong Kong. Hnog Kong.. 2005.

Ho P.L., Mak C.K., Tse C.W., Chow K.H. and Cheung C.H., Invasive Haemophilus influenzae isolates with decreased levofloxacin susceptibility in Hong Kong, Journal of Antimicrobial Chemotherapy. 2006, 57(2): 366.

Ho P.L., Chiu S.S.S., Cheung C.H., Lee R.A., Tsai T.F. and Lau Y.L., Invasive pneumococcal disease burden in Hong Kong children, The Pediatric Infectious Disease Journal. 2006, 25(5): 454-5.

Ho P.L., Cheng J.C., Ching P.T.Y., Kwan J.K., Lim W.W.L., Tong W.C., Wu T.C., Tse C.W., Lam R., Yung R.W.H. and Seto W.H., Optimising antimicrobial prescription in hospitals by introducing an antimicrobial stewardship programme in Hong Kong: consensus statement, Hong Kong Medical Journal. 2006, 12(2): 141-8.

Ho P.L., Should we give pneumococcal conjugate vaccine for all infants in Hong Kong, Annual scientific meeting. Hong Kong Society of Paediatrics. Hong Kong.. 2005.

Kwok R.P., Sun J.Z., Tipoe G.L., Leung Y.H., Yan C.P.K., Ooi C.G.C., Ho P.L., Mak J.C.W., Lam W.K. and Tsang K.W.T., Pseudomonas aeruginosa pyocyanin : discruption of ultrastructure of human respiratory mucosa in vitro, 11th Medical Research Conference, Department of Medicine, The University of Hong Kong,. 2006, Abstract Book 087: pp. 55.

Kwok R.P., Leung Y.H., Sun J.Z., Yan C.P.K., Ooi C.G.C., Ho P.L., Tipoe G.L., Lam W.K. and Tsang K.W.T., The effects of gelomyrtol ForteÒ on human ciliary beat frequency in bronchiectasis subjects in vitro, 11th Medical Research Conference, Department of Medicine, The University of Hong Kong. 2006, Abstract Book 086: pp.54.

Kwok R.P., Leung Y.H., Sun J.Z., Yan C.P.K., Ooi C.G.C., Ho P.L., Tipoe G.L., Lam W.K. and Tsang K.W.T., The effects of gelomyrtol forteÒ on human ciliary beat frequency in bronchiectasis subjects in vitro, Respirology. 2005, 10 (Suppl): A126 (#169).

Kwok R.P., Leung Y.H., Sun J.Z., Yan C.P.K., Ko K.W., Ooi C.G.C., Ho P.L., Tipoe G.L., Lam W.K. and Tsang K.W.T., The effects of myrtol standardized on human ciliary beat frequency in vitro, European Respiratory Medicine. 2005, 26 (Suppl 49): 100S (#714).

Lau S.K.P., Ho P.L., Li W.S., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and Characterization of Chromosomal Class C -Lactamase and Its Regulatory Gene in Laribacter hongkongensis., Antimicrobial Agents and Chemotherapy. 2005, 49: 1957-1964.

Leung T.Y., Ho P.L., Yuen K.Y., Woo W.L., Lam T.H., Kao R.Y.T., Seto W.H. and Yam W.C., Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA, Antimicrobial Agents and Chemotherapy. 2006, 50(3): 1075-8.

Ma E.S. and Ho P.L., Disseminated Penicillium marneffei infection, British Journal of Haematology. 2005, 130(1): 2.

Mak C.K., Ho P.L., Tse C.W., Lau S.K.P. and Wong S.S.Y., Reduced levofloxacin susceptibility and tetracycline resistance in a clinical isolate of Haemophilus quentini identified by 16S rRNA sequencing., Journal of Clinical Microbiology. 2005, 43: 5391-5392.

Mak J.C.W., Ho S.P., Leung Y.H., Ho P.L., Ooi C.G.C., Tipoe G.L., Yan C.P.K., Ip M.S.M., Lam W.K. and Tsang K.W.T., Elevated levels of transforming growth factor-beta(1) in serum of patients with stable bronchiectasis, Respiratory Medicine. 2005, 99: 1223-1228.

Yam W.C., Yuen K.Y., Kam S.Y., Yiu L.S., Chan K.S., Leung C.C., Tam C.M., Ho P.O., Yew W.W., Seto W.H. and Ho P.L., Diagnostic application of genotypic identification of mycobacteria, Journal of Medical Microbiology. 2006, 55(Pt 5): 529-36.

Researcher : Huang K

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Huang Y

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., Genomic evidence for antibiotic resistance genes of actinomycetes as origins of antibiotic resistance genes in pathogenic bacteria simply because actinomycetes are more ancestral than pathogenic bacteria, Medical Hypotheses. 2006, 67: 1297-1304.

Woo P.C.Y., Huang Y., Lau S.K.P., Tsoi H.W. and Yuen K.Y., In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease, Microbiology and Immunology. 2005, 49: 899-908.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Woo P.C.Y., Lau S.K.P., Huang Y., Tsoi H.W., Chan K.H. and Yuen K.Y., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia., Archives of virology. 2005, 150(11): 2299-311.

Researcher : Huang Y

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Researcher : Hui PY

List of Research Outputs

Choi Y.K., Nguyen T.D., Ozaki H., Webby R.J., Puthavathana P., Buranathal C., Chaisingh A., Auewarakul P., Hanh N.T., Ma S.K., Hui P.Y., Guan Y., Peiris J.S.M. and Webster R.G., Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004., Journal of Virology. 2005, 79(16): 10821-5.

Researcher : Hung IFN

List of Research Outputs

Chu C.M., Cheng V.C.C., Hung I.F.N., Chan K.S., Tang S.F., Tsang T.H.F., Chan H.Y. and Yuen K.Y., Viral Load Distribution in SARS Outbreak, Emerging Infectious Diseases. 2005, 11(12): 1882-1886.

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Researcher : Im SWK

Project Title:	Dendritic cell based immune intervention of nasopharyngeal carcinoma
Investigator(s):	Im SWK, Zheng B, Chua DTT
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

N/A

Project Title:	Investigation of community exposure to a novel coronavirus associated with SARS and herd immunity developed among healthy blood donors
Investigator(s):	Im SWK, Yam WC, Lee C.K.
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To determine the exposure of the Hong Kong community to the novel coronavirus and the extent of herd immunity developed; to optimize a cost-effective protocol suitable for high throughput blood testing screening.

List of Research Outputs

Researcher : Kam YW

List of Research Outputs

Kien F.S., Kam Y.W., Roberts A., Cheung C.Y., Lamirande E. .W., Vogel L., Chu S.L., Chan C.M., Tse K.S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Immunization with trimeric S glycoprotein protects from SARS-CoV challenge despite the presence of in vitro facilitating antibodies, Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies 2005 - XIII International Congress of Virology . 2005.

Researcher : Kao RYT

Project Title:	Application of chemical genetic approaches to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei
Investigator(s):	Kao RYT, Yuen KY, Sun H, Madar J.C.S.
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

In conjunction with conventional genetic approaches (such as gene cloning and gene inactivation, etc.), we set out to employ chemical genetic approach - the use of high-throughput screening (HTS) technologies to identify biologically active small molecules that will interfere with particular cellular processes/biological pathways/gene products - to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei. Forward chemical genetics will be used to identify small molecule compounds that will interfere with host-pathogen interactions and dimorphism in P. marneffei. Reverse chemical genetics will be used to identify small molecules that will have specific inhibitory effects on MetAP1 or MetAP2 (essential enzymes involved in removal of N-terminal methionine) of P. marneffei. Resulting specific inhibitors of MetAPs will be tested in in vitro intracellular survival and in vivo animal infection models.

Project Title:	Application of chemical genetic approaches to dissect the molecular basis of virulence and dimorphism in Penicillium marneffei
Investigator(s):	Kao RYT, Yuen KY, Sun H, Madar J.C.S.
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

N/A

Project Title:	Cloning of SARS-associated coronavirus main proteinase (SARS-CoV M^pro and identification of SARS-CoVM^pro specific synthetic substrates
Investigator(s):	Kao RYT, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2004
Completion Date:	01/2006

Abstract:

To develop a fluorescence-based continuous enzymatic assay for the rapid evaluation of potential inhibitors of SARS-CoV M^pro; so as to lay down the foundation for researches in inhibitors of various 3C-like viral proteases.

Project Title:	Identification of small molecule inhibitors of Staphylococcus aureus methionine aminopeptidase by reverse chemical genetics
Investigator(s):	Kao RYT
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005
Completion Date:	12/2006

Abstract:

Staphylococcus aureus forms part of the normal flora of human hosts as a common inhabitant of the nasal tract and the skin of humans and animals (Peacock et al., 2001). S. aureus causes a variety of infections in humans and animals. Infections are initiated when a breach of the skin or mucosal barrier allows S. aureus access to adjoining tissues or the bloodstream. During the pre-antibiotic era, many staphylococcal invasive infections led to the death of the patient. This effect was relieved by the discovery of penicillin in the 1940s. However, by the end of 1950s, approximately 50% of hospital isolates of this bacterium were penicillin resistant. Methicillin and other semi-synthetic derivative of penicillin were then used to treat S. aureus infections. In another few years, methicillin-resistant S. aureus (MRSA) were found in hospitals, leaving vancomycin as the last line of defence against staphylococcal infections (Mann, J. and Crabbe, M.J.C., 1996). However, strains of S. aureus with intermediate resistance to vancomycin are increasingly being found (Projan, S.J., 2000). Clearly, research in the pathogenesis of S. aureus and identification of novel targets for antibacterial therapy against this pathogen is imminent. Co- and post-translational modifications have long been recognized as essential steps for the maturation of proteins for proper folding, regulation, function, targeting and eventually degradation (Bradshaw, 1989; Meinnel, Mechulam, and Blanquet, 1993; Bradshaw, Brickley, and Walker, 1998; Arfin and Bradshaw, 1988). Since protein synthesis begins at the amino-terminus, modification at this site is one of the earliest steps and most common events in protein processing (Lowther and Matthews, 2002). Translation of proteins is initiated with methionine in eukaryotes and N-formyl methionine in prokaryotes, mitochondria, and chloroplasts. The N-formyl group is removed from proteins in prokaryotes and eukaryotic organelles by a deformylase, leaving a methionine residue at the amino terminus (Meinnel, Mechulam, and Blanquet, 1993). However, the majority of mature proteins do not retain the initiator residue. In the cytosolic extract of Escherichia coli only 40% of the polypeptide chains retain the N-terminal methionine, whereas about 50% display alanine, serine, or threonine at their N-termini (Waller, 1963). Methionine aminopeptidase (MetAP) is the enzyme responsible for the catalytic cleavage of the N-terminal methionine from proteins, when the penultimate residue has a small and uncharged side-chain (Tsunasawa, Stewart, and Sherman, 1985; Huang et al., 1987; Hirel et al., 1989). MetAPs have been proven essential in Gram negative bacteria, such as E. coli and Salmonella typhimurium (Chang, McGary, and Chang, 1989; Miller et al., 1989), and in Saccharomyces cerevisiae (Li and Chang, 1995). Its physiological importance has given rise to considerable interest among researchers to design antimicrobial inhibitors that specifically target this enzyme. The vitality of this gene in Gram positive bacteria has just been experimentally illustrated by our group recently (Wong et. al. Manuscript in preparation). The demonstration of map as an essential gene in Staphylococcus aureus has validated map as an attractive drug target for antibacterial and antifungal drug development. Recent advancements in synthetic organic chemistry, molecular biology, and informatics have made possible the use of large collections of small molecules (chemical libraries) to investigate protein/chemical interactions in vitro and in vivo (Kao et al. 2002, 2004; Degterev et al., 2001; Peterson et al., 2000; Mayer et al., 1999). The term "chemical genetics” has been coined to signify the use of chemicals to systematically perturb, and thus determine, the function of proteins in the same way that mutations are used in classical genetics. In forward chemical genetics, small molecules that induce altered phenotypes in cells or organisms are identified and their cellular targets will then be determined subsequently. In reverse chemical genetics, small molecules that interact with a known, purified protein are identified in vitro and their effects on cells or organisms will then be examined. We intend to employ high-throughput screened (HTS) based reverse chemical genetics to identify small molecules that will have specific inhibitory effects on the S. aureus MetAP-I (SaMetAP-I). Resulting specific inhibitors of SaMetAP-I will be tested in cell-based assays (Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pneumoniae, and E. coli) to determine their in vivo antimicrobial effects.

Project Title:	Mode of action of a lead anti-influenza compound identified by chemical genetics
Investigator(s):	Kao RYT
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2006

Abstract:

Based on the concept of chemical genetics and in conjunction with the high-throughput screening (HTS) technologies we have developed, we set out to establish a HTS-based chemical genetic platform for the identification of biologically active small molecule compounds that will perturb most, if not all, biological pathways involved in the pathogenesis of influenza A viruses in cellular models. Forward chemical genetics has been employed to identify small molecule compounds that will interfere with infectivity of human influenza A/WSN/33 (H1N1) in Madin-Darby canine kidney (MDCK) cells. Primary screening of the library has identified more than 1,000 compounds that can perturb the infectivity of H1N1 virus in cell-based assays. A sampling survey of 70 primary hits indicates that ~10% of the hits have EC50 (median effective concentration) at or below 10 microM [determined by MTT (3-[4,5-dimethylth-iazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and PRA (plaque reduction assay)]. The result is in good agreement with the ones we obtained from our previously published SARS-CoV study. One particular lead compound FA5123 {[4-(2-Chloro-4-nitro-phenyl)-2-methyl-piperazin-1-yl]-[3-(2-chloro-phenyl)-5-methyl-isoxazol-4-yl]-methanone} has been identified (figure 1). FA5123 inhibits influenza A/WSN/33 (H1N1) in MDCK cells with an EC50 of 5 microM and TC50 (median toxicity concentration) > 1 mM [determined by MTT (3-[4,5-dimethylth-iazol-2-yl]-2,5-diphenyltetrazolium bromide)-based assay]. The molecular target of this isolated compound will be defined by isolation of mutant viruses resistant to this particular compound followed by identification of the mutated gene by sequencing of the mutant viral gene segments. It is not our major goal to produce ready-to-use, marketable antiviral agents from this study, for that is the job of the pharmaceutical companies. We are exploring novel strategies that can be employed to obtain biologically active novel small-molecule compounds (with defined molecular targets) that will have the potential of being developed to be novel anti-influenza therapeutics.

Project Title:	Methionine aminopeptidase (MetAP) as a novel target for development of antibiotic therapeutics against Staphylococcus aureus (S. aureus) - a proteomic approach
Investigator(s):	Kao RYT, Yuen KY, Che CM, Siu AFM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	02/2006

Abstract:

Using proteomic approach in conjunction with conventional genetic manipulations of S. aureus wild-type strain and its newly constructed MetAP knockout strain, we intend to gain insight into the physiological roles of MetAP. The biomarkers of MetAP deficient state in S. aureus will be studied and be used as future platform for development and validation of MetAP specific antibiotics.

List of Research Outputs

Leung T.Y., Ho P.L., Yuen K.Y., Woo W.L., Lam T.H., Kao R.Y.T., Seto W.H. and Yam W.C., Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA, Antimicrobial Agents and Chemotherapy. 2006, 50(3): 1075-8.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Kao R.Y.T., Lau S.K.P. and Yuen K.Y., Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, FEMS Microbiology Letters. 2005, 252: 57-65.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Researcher : Lam HY

List of Research Outputs

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Researcher : Lam TH

List of Research Outputs

Lee P.P.W., Wong W.H.S., Leung G.M., Chiu S.S.S., Chan K.H., Peiris J.S.M., Lam T.H. and Lau Y.L., Risk-stratified seroperevalence of SARS-coronarvirus (SARS-CoV) among children in Hong Kong, Joint Annual Scientific Meeting, The Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association, 7 January 2006, Queen Elizabeth Hospital. 2006, 44.

Researcher : Lau CY

List of Research Outputs

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

Woo P.C.Y., Lau S.K.P., Lau C.Y., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient, Bone Marrow Transplantation. 2005, 35: 831-833.

Researcher : Lau SKP

Project Title:	Molecular characterization and pathogenesis of a rare bacterium causing psoas abscess
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2005

Abstract:

To characterize the pathogenesis of a rare bacterium associated wth psoas abscess by sequencing its 16S rRNA gene, M protein gene (emm) and streptolysin S structural gene (sagA); to characterize the bacterium's clonality by mulit-locus sequence typing, thus allowing an estimation on the potential of similar diseases in other parts of the world.

Project Title:	Usefulness of microseq 500 16S rDNA identification system for identification of anaerobic gram-positive bacilli isolated from blood cultures
Investigator(s):	Lau SKP, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004
Completion Date:	10/2006

Abstract:

To evaluate the usefulness of the MicroSeq 500 16S rDNA bacterial identification system in the identification of anaerobic gram-positive bacilli isolated from blood cultures; to compare the results from the MicroSeq system with those from commercial biochemical identification kit.

Project Title:	Environmental surveillance of Laribacter hongkongensis, a diarrheal pathogen discovered in Hong Kong
Investigator(s):	Lau SKP, Yuen KY, Woo PCY, Young L
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2004

Abstract:

To conduct a territory-wide, environmental surveillance in Hong Kong to study the epidemiology and potential environmental reservoir of L. hongkongensis.

Project Title:	Antibiotic susceptibility profiles and cloning and characterization of antibiotic resistance genes of Laribacter hongkongensis
Investigator(s):	Lau SKP
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	07/2005

Abstract:

N/A

Project Title:	Antibiotic susceptibility profiles and cloning and characterization of antibiotic resistance genes of Laribacter hongkongensis
Investigator(s):	Lau SKP, Woo PCY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	07/2005

Abstract:

To elucidate the antibiotic susceptibility profile of human and animal strains of L. hongkongensis>; to compare macrobroth dilution method, E-test and disk diffusion test for antibiotic susceptibility testing in L. hongkongensis; to clone and characterize the beta-lactamase and tetracycline resistance genes of L. hongkongensis.

Project Title:	Characterization of two novel anaerobic, gram-positive bacilli recovered from blood cultures of patients
Investigator(s):	Lau SKP, Woo PCY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

The objectives of this study are to characterize two novel anaerobic, gram-positive bacilli recovered from blood cultures of patients in Hong Kong.

Project Title:	Development of a multilocus sequence typing system for Laribacter hongkongensis
Investigator(s):	Lau SKP, Woo PCY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

Understanding the epidemiology of infectious disease pathogens is the first step in combating infectious diseases. This study will help facilitate the comparison of different strains of L. hongkongensis isolated form different hosts or the environment; and in turn, the sources of human infections and possible control measures.

List of Research Outputs

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Lau S.K.P., Woo P.C.Y., Luk W.K., Fung A.M.Y., Hui W.T., Fong A.H.C., Chow C.W., Wong S.S.Y. and Yuen K.Y., Clinical isolates of Streptococcus iniae from Asia are more mucoid and beta-hemolytic than those from North America., Diagnostic Microbiology and Infectious Diseases. 2006, 54: 177-181.

Lau S.K.P., Ho P.L., Li W.S., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and Characterization of Chromosomal Class C -Lactamase and Its Regulatory Gene in Laribacter hongkongensis., Antimicrobial Agents and Chemotherapy. 2005, 49: 1957-1964.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Lau S.K.P., Woo P.C.Y., Li N.K.H., Teng L.L., Leung K.W., Ng K.H.L., Que T.L. and Yuen K.Y., Globicatella bacteremia identified by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology . 2006, 59: 303-307.

Lau S.K.P., To W.K., Tse P.W.T., Chan A.K.H., Woo P.C.Y., Tsoi H.W., Leung A.F.Y., Li K.S.M., Chan P.K.S., Lim W.W.L., Yung R.W.H., Chan K.H. and Yuen K.Y., Human parainfluenza virus 4 outbreak and the role of diagnostic tests, Journal of Clinical Microbiology. 2005, 43: 4515-4521.

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Lau S.K.P., Woo P.C.Y., Chan C.Y.F., Woo W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi, Journal of Clinical Microbiology. 2005, 43: 1470-1472.

Lau S.K.P., Ng K.H.L., Woo P.C.Y., Yip K.T., Fung A.M.Y., Woo G.K.S., Chan K.M., Que T.L. and Yuen K.Y., Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures, Journal of Clinical Pathology. 2006, 59: 219-222.

Mak C.K., Ho P.L., Tse C.W., Lau S.K.P. and Wong S.S.Y., Reduced levofloxacin susceptibility and tetracycline resistance in a clinical isolate of Haemophilus quentini identified by 16S rRNA sequencing., Journal of Clinical Microbiology. 2005, 43: 5391-5392.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, Journal of Clinical Microbiology. 2005, 43: 919-922.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chen Z.W., Wong B.H.L., Chan J.K.H., Wong L.P., He W., Ma C., Chan K.H., Ho D.D. and Yuen K.Y., SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus, Vaccine. 2005, 23: 4959-4968.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess, Systematic and Applied Microbiology . 2005, 28: 316-322.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C.Y., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous abuser with pseudobacteraemia. , Microbiology and Immunology. 2005, 49: 31-39.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Biliary tract disease is a risk factor for Plesiomonas shigelloides bacteremia: a nine-year experience in a Hong Kong hospital and review of the literature, New Microbiologica . 2005, 28: 45-55.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Teng L.L., Lau S.K.P. and Yuen K.Y., Clinical, phenotypic, and genotypic evidence for Streptococcus sinensis as the common ancestor of anginosus and mitis groups of streptococci, Medical Hypotheses . 2005, 66: 345-351.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M.Y., Tang S.F. and Yuen K.Y., Clostridium bacteremia characterized by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology. 2005, 58: 301-307.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Kao R.Y.T., Lau S.K.P. and Yuen K.Y., Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, FEMS Microbiology Letters. 2005, 252: 57-65.

Woo P.C.Y., Lau S.K.P., Teng L.L. and Yuen K.Y., Current status and future directions of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis and traveller's diarrhoea, Current Opinions in Infectious Disease. 2005, 18: 413-419.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., Genomic evidence for antibiotic resistance genes of actinomycetes as origins of antibiotic resistance genes in pathogenic bacteria simply because actinomycetes are more ancestral than pathogenic bacteria, Medical Hypotheses. 2006, 67: 1297-1304.

Woo P.C.Y., Huang Y., Lau S.K.P., Tsoi H.W. and Yuen K.Y., In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease, Microbiology and Immunology. 2005, 49: 899-908.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Infectious diseases emerging from Chinese wet-markets: zoonotic origins of severe respiratory viral infections, Current Opinion in Infectious Diseases. 2006, 19: 401-407.

Woo P.C.Y., Tse H., Wong S.S.Y., Tse C.W., Fung A.M.Y., Tam D.M.W., Lau S.K.P. and Yuen K.Y., Life-threatening invasive Helcococcus kunzii infections in intravenous drug users and ermA-mediated erythromycin resistance., Journal of Clinical Microbiology. 2005, 43: 6205-6208.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Woo P.C.Y., Lau S.K.P., Lau C.Y., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient, Bone Marrow Transplantation. 2005, 35: 831-833.

Woo P.C.Y., Lau S.K.P., Huang Y., Tsoi H.W., Chan K.H. and Yuen K.Y., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia., Archives of virology. 2005, 150(11): 2299-311.

Researcher : Lee P

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Researcher : Lee RA

List of Research Outputs

Ho P.L., Chiu S.S.S., Cheung C.H., Lee R.A., Tsai T.F. and Lau Y.L., Invasive pneumococcal disease burden in Hong Kong children, The Pediatric Infectious Disease Journal. 2006, 25(5): 454-5.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Researcher : Lee SS

List of Research Outputs

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., Development of New Technique for Detection of HIV Specific Cytotoxic T Lymphocytes, Keystone Symposia HIV Vaccines (×6). 27 March-2 April. 2006.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Zhang H. and Lu L., Ex vivo expansion of functional CD4 T cells from HIV patients., The 6th International Workshop on HIV, Cells of Macrophage/Dendritic Lineage and Other Reservoirs.. 2005, 37.

Researcher : Leung CSW

List of Research Outputs

Poon L.L.M., Leung C.S.W., Chan K.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates. , Journal of Medical Virology. 2005, 76: 435-40.

Researcher : Leung CYH

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Leung TY

List of Research Outputs

Leung T.Y., Ho P.L., Yuen K.Y., Woo W.L., Lam T.H., Kao R.Y.T., Seto W.H. and Yam W.C., Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA, Antimicrobial Agents and Chemotherapy. 2006, 50(3): 1075-8.

Researcher : Li KS

List of Research Outputs

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Li KSM

List of Research Outputs

Lau S.K.P., To W.K., Tse P.W.T., Chan A.K.H., Woo P.C.Y., Tsoi H.W., Leung A.F.Y., Li K.S.M., Chan P.K.S., Lim W.W.L., Yung R.W.H., Chan K.H. and Yuen K.Y., Human parainfluenza virus 4 outbreak and the role of diagnostic tests, Journal of Clinical Microbiology. 2005, 43: 4515-4521.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Researcher : Li SMK

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Researcher : Li WS

List of Research Outputs

Lau S.K.P., Ho P.L., Li W.S., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and Characterization of Chromosomal Class C -Lactamase and Its Regulatory Gene in Laribacter hongkongensis., Antimicrobial Agents and Chemotherapy. 2005, 49: 1957-1964.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Kao R.Y.T., Lau S.K.P. and Yuen K.Y., Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, FEMS Microbiology Letters. 2005, 252: 57-65.

Researcher : Li Z

List of Research Outputs

Qian W.P., Tan Y., Chen Y., Peng Y., Li Z., Lu G.X., Lin M.C., Kung H., He M.L. and Shing L.K., Rapid quantification of semen hepatitis B virus DNA by real-time polymerase chain reaction, World Journal of Gastroenterology. 2005, 11(34): 5385-9.

Researcher : Lim WWL

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Ho P.L., Cheng J.C., Ching P.T.Y., Kwan J.K., Lim W.W.L., Tong W.C., Wu T.C., Tse C.W., Lam R., Yung R.W.H. and Seto W.H., Optimising antimicrobial prescription in hospitals by introducing an antimicrobial stewardship programme in Hong Kong: consensus statement, Hong Kong Medical Journal. 2006, 12(2): 141-8.

Niedrig M., Leitmeyer K., Lim W.W.L., Peiris J.S.M., Mackenzie J.S. and Zambon M., First external quality assurance of antibody diagnostic for SARS-new coronavirus, Journal of Clinical Virology. 2005, 34(1): 22-5.

Peiris J.S.M. and Lim W.W.L., Laboratory diagnosis of human disease caused by H5N1 influenza virus, Tropical Update - The Hong Kong College of Pathologists. 2006, 15 (1): 4.

Peiris J.S.M., Tsang D.N.C. and Lim W.W.L., Virological diagnosis of SARS, In: Jane CK Chan, Vivian CW Taam Wong, Challenges of Severe Acute Respiratory Syndrome. Saunders Elsevier, 2005, 1: 299-316.

Shinya K., Hatta M., Yamada S., Takada A., Watanabe S., Halfmann P., Horimoto T., Neumann G., Kim J.H., Lim W.W.L., Guan Y. and Peiris J.S.M., Characterization of a human H5N1 influenza A virus isolated in 2003., Journal of Virology. 2005, 79(15): 9926-32.

Researcher : Lu H

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Luk WK

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Luk W.K., Fung A.M.Y., Hui W.T., Fong A.H.C., Chow C.W., Wong S.S.Y. and Yuen K.Y., Clinical isolates of Streptococcus iniae from Asia are more mucoid and beta-hemolytic than those from North America., Diagnostic Microbiology and Infectious Diseases. 2006, 54: 177-181.

Researcher : Ma HT

List of Research Outputs

Poon L.L.M., Wong W.Y., Ma H.T., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S. and Peiris J.S.M., Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification, Clinical Chemistry. 2006, 52(2): 303-6.

Researcher : Ma SK

List of Research Outputs

Choi Y.K., Nguyen T.D., Ozaki H., Webby R.J., Puthavathana P., Buranathal C., Chaisingh A., Auewarakul P., Hanh N.T., Ma S.K., Hui P.Y., Guan Y., Peiris J.S.M. and Webster R.G., Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004., Journal of Virology. 2005, 79(16): 10821-5.

Researcher : Ma SSL

List of Research Outputs

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, Journal of Clinical Microbiology. 2005, 43: 919-922.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Kao R.Y.T., Lau S.K.P. and Yuen K.Y., Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, FEMS Microbiology Letters. 2005, 252: 57-65.

Researcher : Mak CK

List of Research Outputs

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

Ho P.L., Mak C.K., Tse C.W., Chow K.H. and Cheung C.H., Invasive Haemophilus influenzae isolates with decreased levofloxacin susceptibility in Hong Kong, Journal of Antimicrobial Chemotherapy. 2006, 57(2): 366.

Mak C.K., Ho P.L., Tse C.W., Lau S.K.P. and Wong S.S.Y., Reduced levofloxacin susceptibility and tetracycline resistance in a clinical isolate of Haemophilus quentini identified by 16S rRNA sequencing., Journal of Clinical Microbiology. 2005, 43: 5391-5392.

Researcher : Ng F

List of Research Outputs

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., Development of New Technique for Detection of HIV Specific Cytotoxic T Lymphocytes, Keystone Symposia HIV Vaccines (×6). 27 March-2 April. 2006.

Researcher : Ng IHY

List of Research Outputs

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Differential expression of chemokines and their receptors in adult and neonatal macrophages infected with human or avian influenza viruses, 6^th Guangdong - Hong Kong Paediatric Exchange Meeting, Guangdong, PRC, 25 June 2006 (2^nd Prize in Poster Presentation). 2006, 38.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Functional Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Production by Avian Influenza Virus-Infected Macrophages, The Journal of Infectious Diseases. 2006, 193(7): 945-953.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Impairment of T lymphocytes activation in avian influenza: an in vitro study, 6th Guangdong – Hong Kong Paediatric Exchnage Meeting. China, 2006, 39.

Researcher : Ng MH

List of Research Outputs

Ma L., Chan K.W., Trendell-Smith N.J., Wu A.Y.Y., Tian L., Lam A.C., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam C.S.K., Li X., Yang C., Leung S.Y., Ng M.H. and Huang F., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic cells in susceptible mouse strains, In: Ma L, Chan KW, Trendell-Smith NJ, Wu A, Tian L, Lam AC, Chan AK, Lo CK, Chik S, Ko KH, To CK, Kam SK, Li XS, Yang CH, Leung SY, Ng MH, Stott DI, MacPherson GG, Huang FP, European Journal of Immunology (In This Issue highlight, Editorial Comments). 2005, 35(11): 3364-75.

Zheng Y., Cao K., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H., Lu L. and Zheng B., Complementary activation of peripheral natural killer cell immunity in nasopharyngeal carcinoma, Cancer Science. 2006, 97(9): 912-9.

Researcher : Ng SF

List of Research Outputs

Poon L.L.M., Wong W.Y., Chan K.H., Ng S.F., Yuen K.Y., Guan Y. and Peiris J.S.M., Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. , Journal of Clinical Microbiology. 2005, 43: 3457-3459.

Researcher : Ng SP

Project Title:	Monoclonal antibodies against lipopolysaccharide antigens of Salmonella and Shigella spp.
Investigator(s):	Ng SP
Department:	Microbiology
Source(s) of Funding:	Research Initiation Programme
Start Date:	02/2001

Abstract:

To characteerise a panel of monoclonal antibodies raised against the outer core region of Salamonella lipopolysaccharides (LPS).

Project Title:	Collection of background information on the alternative faecal indicators in the environmental waters of Hong Kong and their correlation with Escherichia coli
Investigator(s):	Ng SP, Yam WC
Department:	Microbiology
Source(s) of Funding:	Environment Protection Department - General Award
Start Date:	06/2001

Abstract:

To detect and enumerate alternative faecal microbial indicator organisms in the environmental waters of Hong Kong and to correlate the results with Escherichia coli in order to assess their value in water quality monitoring.

List of Research Outputs

Lam R.C.W., Leung M.K.H., Leung Y.C., Vrijmoed L.L.P., Yam W.C., Ng S.P. and Chu P.K., Photocatalytic glass reactor for solar-assisted air purification, Proceedings of the 2005 Solar World Congress. Orlando, Florida, USA, 2005, CD-ROM.

Leung M.K.H., Liu C.H., Chan A.H.S., Leung Y.C., Yam W.C., Ng S.P. and Vrijmoed L.L.P., Prediction of transient turbulent dispersion by CFD-statistical hybrid modeling method, Atmospheric Environment. Oxford, UK, Elsevier Ltd., 2005, 39: 6345-6351.

Zheng Y., Cao K., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H., Lu L. and Zheng B., Complementary activation of peripheral natural killer cell immunity in nasopharyngeal carcinoma, Cancer Science. 2006, 97(9): 912-9.

Researcher : Peiris JSM

Project Title:	The application of DNA arrays to characterise the avian influenza gene pool in southern China
Investigator(s):	Peiris JSM, Guan Y, Shortridge KF, Yang MMS, Li K.S.
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2002

Abstract:

This project is a multi-institutional collaborative study for developing a high throughput DNA array to allow genotyping of influenza virus isolates of avian origin from Hong Kong and applying it to characterize the influenza virus gene pool in sourthern China. Through its application, it is expected to:- 1) identify the precursor virus that donated its internal genes to generate the mutiple reassortment of H5N1 isolated in Hong Kong markets in 2001; 2) define the frequency of reassortment between viruses of different virus lineages that co-circulate in poultry in Hong Kong and correlate this to host-specificity; and 3) investigate whether avian internal gene segments are transiently introduced into the human influenza gene pool.

Project Title:	Influenza pandemic preparedness in Asia (Research collaboration with national Institute of Allergy and Infectious Diseases (NIAID), St. Jude Children's Research Hospital, Inc. (SJCRH) and The University of Hong Kong)
Investigator(s):	Peiris JSM, Guan Y
Department:	Microbiology
Source(s) of Funding:	National Institutes of Health, US Department of Health and Human Services - General Award
Start Date:	04/2003

Abstract:

To study influenza pandemic preparedness in Asia.

Project Title:	Virus-cell interaction and the pathogenesis of SARS
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To explore what is the biological basis for the selective attraction of macrophages to the lung; to study what immunopathological consequences follow.

Project Title:	Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?
Investigator(s):	Peiris JSM, Guan Y, Lau ASY, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

To identify the viral genetic determinants responsible for the differential cytokine hyper-induction associated with H5N1/97 viruses. We have already established the role of the viral NS gene, however, a role for other viral genes has not been excluded. we hypothesize (and have preliminary evidence) that one or more other viral genes are also involved in the hyper-induction of cytokines; to determine whether the avian viral genes/gene products of H5N1/97 directly up-regulate cytokine induction or lack "immune evasion" mechanisms evolved by human influenza viruses? to determine whether the differential up-regulation of cytokines associated with H5N1/97 is also observed in human lung epithelial cells (primary bronchial epithelial cells and human lung epithelial cell lines e.g. A549 cells.

Project Title:	Viral determinants underlying the increased secretion of pro-inflammatory cytokines in H5N1 infected human macrophages: a mechanism for the unusual severity of human H5N1 disease?
Investigator(s):	Peiris JSM, Guan Y, Lau ASY, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

N/A

Project Title:	The role of influenza virus gene constellation and viral morphology on cytokine induction, pathogenesis and viral virulence
Investigator(s):	Peiris JSM, Nicholls JM, Guan Y, Poon LLM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	02/2005

Abstract:

To correlate the cytokine induction phenotype with viral genotype and morphology.

Project Title:	Virology and pathogenesis of avian influenza and SARS
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Senior Medical Research Fellowships
Start Date:	03/2005
Completion Date:	08/2005

Abstract:

The main objectives of this project are: 1) To study the pathogenesis and clinical disease burden of influenza. 2) To consolidate this by achieving international recognition as a WHO Influenza Collaborating Center for Animal influenza viruses.

Project Title:	The macrophage in the pathogenesis of Severe Acute Respiratory Syndrome Coronavirus infection
Investigator(s):	Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

The global gene expression profiling of SARS-CoV infected macrophages will provide the basis for a more detailed analysis of the signaling pathways involved in subsequent studies, and may provide rational and novel therapeutic options to be devised for treating SARS.

Project Title:	Mass Tag PCR: A novel, multiplex and potentially sensitive approach for the rapid detection of respiratory pathogens
Investigator(s):	Peiris JSM, Chiu SSS, Cheung CY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	06/2006

Abstract:

To divise, optimize and evaluate the Mass Tag PCR system for the rapid disgnosis for simultaneous screening of a wide spectrum of causative agents for human respiratory tract infections, including the avian influenza H5N1 and H7N7, SARS-coronavirus (SARS-CoV). Thiw will significantly facilitate better prevention, treatment and control of infectious diseases.

Project Title:	Pathogenesis, cell signaling and virus evolution of avian influenza A (H5N1)
Investigator(s):	Peiris JSM, Guan Y, Ip NT, Yang MMS, Lau ASY, Nicholls JM, Chen H, Poon LLM, Lau YL, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Central Allocation Vote - Group Research Project
Start Date:	06/2006

Abstract:

To study the continued evolution of H5N1 influenza viruses in Vietnam and Indonesia, in the context of the ongoing studies in Hong Kong and mainland China; to define differences in cell-signaling pathways activated by avian influenza A H5N1 compared to convetional human influenza (H1N1 or H3N2) in primary human macrophages and epithelial cells; to compare the biological characteristics of H5N1 viruses from Indonesia and Vietnam to define a basis for possible differential pathogenicity for humans; investigate the biological significance of specific viral genetic mutations/changes in recent h5N1 viruses in primary human cell culture models

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Chan M.C.W. and Peiris J.S.M., Induction Of Proinflammatory Cytokines And Chemokines By Influenza A (H5N1) Viruses In Human Alveolar Epithelial Cells (typeII Pneumocytes), Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies 2005.

Chan M.C.W., Cheung C.Y., Chui W.H., Tsao G.S.W., Nicholls J.M., Chan Y.O., Chan W.Y., Long H.T., Poon L.L.M., Guan Y. and Peiris J.S.M., Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells, Respiratory Research. 2005, 6: 135.

Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Chan K.H., Mak W.W., Ngan H.Y.S., Xu X., Screaton G., Tam P.K.H., Austyn J.M., Chan L.C., Yip S.P., Peiris J.S.M., Khoo U.S. and Lin C.L., Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection. , Nature Genetics. 2006, 38: 38-46.

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Choi Y.K., Nguyen T.D., Ozaki H., Webby R.J., Puthavathana P., Buranathal C., Chaisingh A., Auewarakul P., Hanh N.T., Ma S.K., Hui P.Y., Guan Y., Peiris J.S.M. and Webster R.G., Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004., Journal of Virology. 2005, 79(16): 10821-5.

Chong W.P., Ip E.W.K., Tso H.W., Ng M.W., Wong W.H.S., Law H.K.W., Yung R.W.H., Chow E.Y., Au K.L., Chan E.Y.T., Lim W., Peiris J.S.M. and Lau Y.L., The interferon gamma gene polymorphism +874 A/T is associated with severe acute respiratory syndrome, BMC Infectious Diseases. 2006, 6: 82.

Ellis T.M., Sims L.D., Wong H.K., Wong C.W., Dyrting K.C., Chow K.W., Leung C. and Peiris J.S.M., Use of avian influenza vaccination in Hong Kong, Developments in Biologicals. 2006, 124: 133-43.

Hui S.L., Chu L.W., Peiris J.S.M., Chan K.H., Chu D. and Tsui W., Immune response to influenza vaccination in community-dwelling Chinese elderly persons., Vaccine. 2006, 24(25): 5371-80.

Hulse-Post D.J., Sturm-Ramirez K.M., Humberd J., Seiler P., Govorkova E.A., Krauss S., Scholtissek C., Puthavathana P., Buranathai C., Nguyen T.D., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Role of domestic ducks in the propagation and biological evolution of highly pathogenic H5N1 influenza viruses in Asia., Proceedings of the National Academy of Sciences of the United States of America. 2005, 102(30): 10682-7.

Jennings J.C. and Peiris J.S.M., Avian influenza H5N1: is it a cause for concern?, Internal Medicine Journal. 2005, 36: 145-147.

Khoo U.S., Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Ngan H.Y.S., Tam P.K.H., Chan L.C., Yip S.P., Peiris J.S.M., Sham P.C. and Lin C.L., The protective role of homozygous L-SIGN (CLEC4M) in SARS coronavirus infection., HUGO's 11th Human Genome Meeting, Helsinki, Finland. . 2006.

Kien F.S., Kam Y.W., Roberts A., Cheung C.Y., Lamirande E. .W., Vogel L., Chu S.L., Chan C.M., Tse K.S., Subbarao K., Peiris J.S.M., Nal-Rogier B.T.M. and Altmeyer R.M., Immunization with trimeric S glycoprotein protects from SARS-CoV challenge despite the presence of in vitro facilitating antibodies, Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies 2005 - XIII International Congress of Virology . 2005.

Kuiken T., Leighton F.A., Fouchier R.A., LeDuc J.W., Peiris J.S.M., Schudel A., Stohr K. and Osterhaus A.D., Public Health. Pathogen surveillance in animals., Science. 2005, 309: 1680-1.

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Lau Y.L., Ng M.W., Peiris J.S.M., Pak S., Yung W.H., Chow Y., Au K.L., Chan Y.T., Lim W., Bonnevie-Nielsen V. and Field L.L., Innate immune response genes polymorphisms in SARS, 1^st Congress of Asian Society for Pediatric Research, Tokyo, Japan, 24-26 November 2005. 50.

Lau Y.L. and Peiris J.S.M., Pathogenesis of severe acute respiratory syndrome, In: Lau YL, Peiris JS, Current Opinion in Immunology. 2005, 17(4): 404-410.

Lee D.C.W., Mok K.P., Cheung C.Y., Law H.Y., Peiris J.S.M. and Lau A.S.Y., Mechanisms of H5N1 Avian Influenza Virus Induced TNF-alpha dysregulation and a Delay in Apoptosis in Primary Blood Macrophages, Cell Research (The 2005 Annual Meeting of International Society for Interferon & Cytokine Research), Shanghai, PR China, 20-24 October 2005. 15(10) Suppl: 100.

Lee D.C.W., Mok K.P., Cheung C.Y., Law H.Y., Peiris J.S.M. and Lau A.S.Y., The Paradox of H5N1 Avian Influenza Virus Infection in Primary Blood macrophages: TNF-alpha Hyper-induction and a Delay in Apoptosis. * Recipient of 2nd Prize Young Investigator Award., In: Davy C.W. Lee, Chris K.P. Mok, Chung-Yan Cheung, Anna H.Y. Law, Malik Peiris and Allan S.Y. Lau., 7th World Congress on Inflammation, 20 - 24 August 2005 in Melbourne, Australia.. 2005.

Lee D.C.W., Mok K.P., Cheung C.Y., Law H.Y., Peiris J.S.M. and Lau A.S.Y., The Paradox of H5N1 Avian Influenza Virus Infection in Primary Blood macrophages: TNF-alpha Hyper-induction and a Delay in Apoptosis, Inflammation Research (Official Journal of The International Societies, The European Histamine Research Society), 23 August 2005. 54: S181.

Lee D.C.W., Cheung C.Y., Law H.Y., Mok K.P., Peiris J.S.M. and Lau A.S.Y., p38 Mitogen-Activated Protein Kinase-Dependent Hyperinduction of Tumor Necrosis Factor Alpha Expression in Reponse to Avian Influenza Virus H5N1, Journal of Virology. 2005, 79(16): 10147-10154.

Lee P.P.W., Wong W.H.S., Leung G.M., Chiu S.S.S., Chan K.H., Peiris J.S.M., Lam T.H. and Lau Y.L., Risk-Stratified Seroprevalence of Severe Acute Respiratory Syndrome Coronavirus Among Children in Hong Kong, Pediatrics. 2006, 117(6): e1156-1162.

Lee P.P.W., Wong W.H.S., Leung G.M., Chiu S.S.S., Chan K.H., Peiris J.S.M., Lam T.H. and Lau Y.L., Risk-stratified seroperevalence of SARS-coronarvirus (SARS-CoV) among children in Hong Kong, Joint Annual Scientific Meeting, The Hong Kong Paediatric Society and Hong Kong Paediatric Nurses Association, 7 January 2006, Queen Elizabeth Hospital. 2006, 44.

Merianos A. and Peiris J.S.M., International health regulations (2005)., The Lancet. 2005, 366(9493): 1249-51.

Nicholls J.M. and Peiris J.S.M., Good ACE, bad ACE do battle in lung injury, SARS, Nature Medicine. 2005, 11: 821-822.

Nicholls J.M., Butany J., Poon L.L.M., Chan K.H., Beh S.L., Poutanen S., Peiris J.S.M. and Wong M.P., Time course and cellular localization of SARS-CoV nucleoprotein and RNA in lungs from fatal cases of SARS, Public Library of Science Medicine . 2006, 3(2): e27.

Niedrig M., Leitmeyer K., Lim W.W.L., Peiris J.S.M., Mackenzie J.S. and Zambon M., First external quality assurance of antibody diagnostic for SARS-new coronavirus, Journal of Clinical Virology. 2005, 34(1): 22-5.

Osterhaus A.D.M.E. and Peiris J.S.M., Lessons Learnt, In: Malik Peiris, Larry J. Anderson, Albert D.M.E. Osterhaus, Klaus Stohr and K.Y. Yuen, Severe Acute Respiratory Syndrome. Blackwell Publishing, 2005, 1: 249-253.

Peiris J.S.M., Advisor., Asia Pacific Advisory Committee on Influenza.. 2005.

Peiris J.S.M. and Osterhaus A.D.M.E., Aetiology of SARS, In: Malik Peiris, Larry J. Anderson, Albert D.M.E. Osterhaus, Klaus Stohr and K.Y. Yuen, Severe Acute Respiratory Syndrome. Blackwell Publishing, 2005, 1: 50-57.

Peiris J.S.M., Avian Influenza in Human and Animal., Influenza Inter-Pandemic Preparedness Plan Episode III. Phuket, Thailand.. 2006.

Peiris J.S.M., Avian Influenza update, Asia Pacific Advisory Committee on Influenza (APACI), Hong Kong.. 2005.

Peiris J.S.M., Avian Influenza, Talk to the key decision makers of the Bank in Asia of the HSBC, Hong Kong.. 2005.

Peiris J.S.M., Avian influenza update, Asia Pacific Advisory Committee on Influenza Meeting. Hong Kong. 8-9 October, 2005.. 2005.

Peiris J.S.M., Avian influenza: a pandemic threat, 4th World Congress of The World Society for Paediatric Infectious Diseases. Scientific session of the Asian Society for Paediatric Infectious Diseases (ASPID): Emerging Infections in Asia. Warshaw, Poland.. 2005.

Peiris J.S.M., Bird Flu, Asia Society Hong Kong.. 2006.

Peiris J.S.M., Chairman of "Pandemic preparedness" Session, The Lancet Asia Medical Forum 2006. Singapore.. 2006.

Peiris J.S.M., Chairman of Plenary Lecture "Influenza Pandemics: Current rends and where they will likely lead in the future", The 2nd International Infection Control Conference, Hong Kong. 2006.

Peiris J.S.M., Co-organizer , 2nd Pasteur - Asia Virology Course - Respiratory Viruses, Hong Kong.. 2005.

Peiris J.S.M., Co-organizer of the course., 2nd Pasteur – Asia Virology Course – Respiratory Viruses. Institute of Pasteur, The University of Hong Kong. . 2005.

Peiris J.S.M., Cytokine induction in murine macrophages by influenza A infection., Thesis of M.Phil. student Ms Chan Wan Yi.. 2005.

Peiris J.S.M., Detection of human coronavirus OC43 and 229E by reverse transcription PCR in children hospitalized with respiratory disease in Hong Kong., Thesis of MMedSci. student Ms. Grace Kwan.. 2005.

Peiris J.S.M., Anderson L.J., Osterhaus A.D.M.E., Stohr K. and Yuen K.Y., Editor, "Severe Acute Respiratory Syndrome". Blackwell Publishing, 2005, 1: 1-263.

Peiris J.S.M., Emerging Viral Infections, 2006 RACP Congress. Cairns, Australia. 2006.

Peiris J.S.M., Expert Advisor., Technical Review and International Expert Consultation Meeting of the Avian Influenza Situation in Indonesia. WHO Indonesia.. 2006.

Peiris J.S.M., Expert Advisor., WHO sponsored "Expert group meeting to standardize Avian Influenza diagnosis in referral laboratories in India. WHO Pune.. 2006.

Peiris J.S.M., Genesis and prevalence of H1N2 swind influenza virus in pigs from Southern China., Thesis of MMedSci student Mr Edward Ma.. 2005.

Peiris J.S.M., H5N1 influenza virus, The International Symposium on "Molecular Bases Underlying Microbial Infections and the Host Responses", Tokyo, Japan.. 2005.

Peiris J.S.M., Human H5N1 Disease, "Roche Satellite Symposium" of the Lancet Asia Medical Forum. Singapore.. 2006.

Peiris J.S.M., Human Infections with Highly Pathogeneics Avian Influenza Viruses, 2006 Joint Keystone Symposium on "Viral Immunity" From Basic Mechanisms to Vaccines" and "Advances in Influenza Research: From Birds to Bedsice". Colorado, USA.. 2006.

Peiris J.S.M., Human infections with avian influenza viruses, Keystone Symposia. Joint Plenary Session. Advances in Influenza Research: From birds to bench to bedside and Viral Immunity: From basic mechanisms to vaccines, Colorado, USA.. 2006.

Peiris J.S.M., ICAAC program committee member for e years from Nov. 2005 to Oct. 2008., ICAAC, USA.. 2005.

Peiris J.S.M. and Yuen K.Y., Influenza, In: Mary Ip, Moira Chan-Yeung, Wah Kit Lam, Nan Shan Zhong, Respiratory Medicine: An Asian Perspective. Hong Kong University Press, 2005, 1: 209-221.

Peiris J.S.M., Keynote speaker: Lecture "Avian Influenza", University of Indonesia, Jakarta. 2005.

Peiris J.S.M. and Lim W.W.L., Laboratory diagnosis of human disease caused by H5N1 influenza virus, Tropical Update - The Hong Kong College of Pathologists. 2006, 15 (1): 4.

Peiris J.S.M., Laboratory diagnosis of respiratory infections & Aetiology of Severe Acute Respiratory Syndrome., 2nd Pasteur - Asia Virology Course - Respiratory Viruses, Hong Kong.. 2005.

Peiris J.S.M., Lecture on Avian Influenza., University of Indonesia. Jakarta, Indonesia.. 2006.

Peiris J.S.M., Lecture: Avian Influenza update. , Asia Pacific Advirosy Committee on Influenza (APACI). Hong Kong. . 2005.

Peiris J.S.M., Lecture: H5N1 influenza virus., The International Symposium on “Molecular Bases Underlying Microbial Infections and the Host Responses”. Tokyo, Japan. . 2005.

Peiris J.S.M., Lecture: Laboratory diagnosis of respiratory infections & Aetiology of Severe Acute Respiratory Syndrome, 2nd Pasteur – Asia Virology Course – Respiratory Viruses. Hong Kong. . 2005.

Peiris J.S.M., Lecturer., WHO Indonesia Training Course, Bogor, Indonesia.. 2005.

Peiris J.S.M., Lecturer., WHO Training Course on rapid diagnosis of Avian Influenza, Sri Lanka.. 2005.

Peiris J.S.M., Member for 4 years wef Feb. 2006., WHO Expert Advisory Panel on Virus Diseases. Geneva, Switzerland.. 2006.

Peiris J.S.M., Member., Working Group on Investigating Interventions in the case of an Influenza Pandemic of the Scientific Committee on Advanced Data Analysis and Disease Modelling, Centre for Health Protection, Department of Health. Hong Kong. 2005.

Peiris J.S.M., Pandemic Influenza: A Potential Role for Statins in Treatment and Prophylaxis, Clinical Infectious Diseases Award for Outstanding Review. 2006.

Peiris J.S.M., Pathogenesis of avian flu H5N1., Centre for Health Protection. Seminar: Update on Avian Influenza and the Use of Anti-viral Agents. Hong Kong.. 2006.

Peiris J.S.M., Pathogenesis, The Lancet Asia Medical Forum 2006. Singapore.. 2006.

Peiris J.S.M., Plenary Lecture: Emerging viral infections including influenza, 8th Nagasaki-Sinagpore Medical Symposium on Infectious Diseases, Singapore.. 2006.

Peiris J.S.M., SARS, avian influenza and innate immune resposes, Keystone Symposium. Determinants of host resistance, susceptibility or immunopathology to pathogens, Colorado, USA.. 2006.

Peiris J.S.M., Scientific Advisory Committee., VIII International Symposium on Respiratory Viral Infections, Hawaii, USA.. 2006.

Peiris J.S.M., Scientific session of the Asian Society for Pediatric Infectious Diseases (ASPID): Emerging Infections in Asia. Lecture: Avian influenza: a pandemic threat., 4th World Congress of The World Society for Pediatric Infectious Diseases. Warsaw, Poland. . 2005.

Peiris J.S.M., Session IV Pathogenesis; Lecture: "SARS Pathogenesis: Insights from Human Experience", VIII International Symposium on Respiratory Viral Infections, Hawaii, USA. . 2006.

Peiris J.S.M., Session: Avian Influenza; Lecture: Transmission from Human to Human: Evidence or Reality., 2nd International Infection Control Conference. Hong Kong.. 2006.

Peiris J.S.M., Session: Influenza: Lecture: Avian Flu: A Threat to Human Health?, CU 3rd CEID Annual Scientific Meeting Scientific Programme: The Changing Scene of Endemic, Epidemic and Pandemic Infections. Hong Kong.. 2006.

Peiris J.S.M., Session: Innovation and strategy against Avian Influenza. Lecture on Avian Flu., 2006 Shanghai Bio-forum. Shanghai, China.. 2006.

Peiris J.S.M., Temporary Advisor., Regional Meeting on Avian Influenza and Pandemic Preparedness, Bangkok, Thailand.. 2005.

Peiris J.S.M. and Poon L.L.M., The SARS coronavirus (SARS-CoV)., In: SARS: How a global epidemic was stopped. World Health Organization. Geneva.. Mackenzie, 2006, 210-217.

Peiris J.S.M., Poon L.L.M. and Mackenzie J.S., The SARS coronavirus (SARS-CoV), SARS - How a global epidemic was stopped. World Health Organization, 2006, 210-217.

Peiris J.S.M., Viral Infection., 13th Infection Control Course for Health Care Professionals 2006. Hong Kong.. 2006.

Peiris J.S.M., Tsang D.N.C. and Lim W.W.L., Virological diagnosis of SARS, In: Jane CK Chan, Vivian CW Taam Wong, Challenges of Severe Acute Respiratory Syndrome. Saunders Elsevier, 2005, 1: 299-316.

Peiris J.S.M., Virus Plenary: Emerging virus diseases. Topic: Confronting SARS: A view from the epicentre., Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies 2005. XIII International Congress of Virology. San Francisco, U.S.A.. 2005.

Peiris J.S.M., Virus Plenary: Emerging virus diseases; Lecture: Confronting SARS: A view from the epicentre, Joint Meeting of the 3 Divisions of the International Union of Microbiological Societies 2005, San Francisco, USA. 2005.

Poon L.L.M., Wong W.Y., Chan K.H., Ng S.F., Yuen K.Y., Guan Y. and Peiris J.S.M., Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. , Journal of Clinical Microbiology. 2005, 43: 3457-3459.

Poon L.L.M., Leung C.S.W., Chan K.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates. , Journal of Medical Virology. 2005, 76: 435-40.

Poon L.L.M., Wong W.Y., Ma H.T., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S. and Peiris J.S.M., Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification, Clinical Chemistry. 2006, 52(2): 303-6.

Shinya K., Hatta M., Yamada S., Takada A., Watanabe S., Halfmann P., Horimoto T., Neumann G., Kim J.H., Lim W.W.L., Guan Y. and Peiris J.S.M., Characterization of a human H5N1 influenza A virus isolated in 2003., Journal of Virology. 2005, 79(15): 9926-32.

Sturm-Ramirez K.M., Hulse-Post D.J., Govorkova E.A., Humberd J., Seiler P., Puthavathana P., Buranathai C., Nguyen T.D., Chaisingh A., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Are ducks contributing to the endemicity of highly pathogenic H5N1 influenza virus in Asia?, Journal of Virology. 2005, 79(17): 11269-79.

Uiprasertkul M., Puthavathana P., Sangsiriwut K., Pooruk P., Srisook K., Peiris J.S.M., Nicholls J.M., Chokephaibulkit K., Vanprapar N. and Auewarakul P., Influenza A H5N1 replication sites in humans, Emerging Infectious Diseases. 2005, 11(7): 1036-1041.

Webster R.G., Peiris J.S.M., Chen H. and Guan Y., H5N1 outbreaks and enzootic influenza, Emerging Infectious Diseases. 2006, 12(1): 3-8.

Webster R.G., Guan Y., Poon L.L.M., Krauss S., Webby R., Govorkovai E. and Peiris J.S.M., The spread of the H5N1 bird flu epidemic in Asia in 2004, Archives of Virology Supplementum. 2005, 19: 117-29.

Wong C.M., Chan K.P., Yang L., Leung G.M., Chan K.H., Hedley A.J. and Peiris J.S.M., Influenza associated weekly hospitalization in Hong Kong (Abstract), 17th IEA World Congress of Epidemiology, 21-25 August 2005, Bangkok, Thailand. Bangkok, Thailand, International Epidemiological Association, 2005, 383.

Wong C.M., Yang L., Chan K.P., Leung G.M., Chan K.H., Guan Y., Lam T.H., Hedley A.J. and Peiris J.S.M., Influenza-associated hospitalization in a subtropical city, PLoS Medicine. 2006, 3(4): e121.

Wong C.M., Peiris J.S.M., Thach T.Q., Chau Y.K., Chan K.P., Chung R.Y.N., Thomas G.N., Lam T.H., Wong T.W. and Hedley A.J., Interaction between air pollution and respiratory viruses: Time-series for daily mortality and hospital admissions, US Health Effects Insitute PAPA Hong Kong Regional Meeting, 30 November - 1 December 2005, Hong Kong (powerpoint presentation). Hong Kong, US Health Effects Institute, 2005.

Wong C.M., Peiris J.S.M., Thach T.Q., Chau Y.K., Chan K.P., Chung R.Y.N., Thomas G.N., Lam T.H., Wong T.W. and Hedley A.J., Multi-city PAPA - Hong Kong study, US Health Effects Institute PAPA Hong Kong Regional Meeting, 30 November - 1 December 2005, Hong Kong (powerpoint presentation). Hong Kong, US Health Effects Institute, 2005.

Wong C.M., Peiris J.S.M., Thach T.Q., Chau Y.K., Chan K.P., Thomas G.N., Lam T.H., Wong T.W. and Hedley A.J., Public health and air pollution in Asia (PAPA) project - Multicity and Hong Kong studies (Powerpoint presentation), 46th Annual Meeting of Japan Society of Atmospheric Environment: Understanding Exposure to and Health Effects of Air Pollution: The Public Health and Air Pollution in Asia (PAPA) Program, 8 September 2005, Nagoya, Japan. Nagoya, Japan, Health Effects Institute, 2005.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Xu M.S., Chan Y.K., Peiris J.S.M., Yip S.P., Cheung A.N.Y. and Khoo U.S., A variant in the CD209 promoter is associated with severity of Severe Acute Respiratory Syndrome (SARS), HUGO's 11th Human Genome Meeting, Helsinki, Finland. 2006.

Yang L., Wong C.M. and Peiris J.S.M., Seasonal health impacts of influenza in Hong Kong (Abstract), Hong Kong College of Community Medicine Annual Scientific Meeting, 25 June 2006, Hong Kong. Hong Kong, Hong Kong College of Community Medicine, 2006, Poster 12.

Yang L., Wong C.M., Chan K.P. and Peiris J.S.M., The impact of influenza on hospitalization in Hong Kong (Abstract), 10th Research Postgraduate Symposium, 3 December 2005, Hong Kong. Hong Kong, Faculty of Medicine, University of Hong Kong, 2005, 85.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Differential expression of chemokines and their receptors in adult and neonatal macrophages infected with human or avian influenza viruses, 6^th Guangdong - Hong Kong Paediatric Exchange Meeting, Guangdong, PRC, 25 June 2006 (2^nd Prize in Poster Presentation). 2006, 38.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Functional Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Production by Avian Influenza Virus-Infected Macrophages, The Journal of Infectious Diseases. 2006, 193(7): 945-953.

Zhou J., Law H.K.W., Cheung C.Y., Ng I.H.Y., Peiris J.S.M. and Lau Y.L., Impairment of T lymphocytes activation in avian influenza: an in vitro study, 6th Guangdong – Hong Kong Paediatric Exchnage Meeting. China, 2006, 39.

de Jong M.D., Thanh T.T., Khanh T.T., Hien V.M., Smith G.J., Chau N.V., Cam B.V., Qui P.V., Ha D.Q., Guan Y., Peiris J.S.M., Hien T.T. and Farrar J., Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection, New England Journal of Medicine. 2005, 353: 2667-2672.

Researcher : Poon LLM

Project Title:	Reverse genetic for a coronavirus responsible for severe acute respiratory syndrome
Investigator(s):	Poon LLM, Guan Y
Department:	Microbiology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To generate a coronavirus-like mini vRNA GFP (green fluorescent protein) reporter system to study the transcription and replication of coronavirus; to establish a reverse genetic system of coronavirus.

Project Title:	Roles of segment-specific vRNA sequences of influenza A virus in viral replication, transcription and gene expression
Investigator(s):	Poon LLM, Guan Y, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2003

Abstract:

To establish a GFP reporter assay for influenza replication studies; to determine the potential function of segment-specific viral RNA sequences in viral replication, tansporation and gene expression; to study the 5' and 3' segment-specific regions of avian, human and swine influenza.

Project Title:	Roles of segment-specific vRNA sequences of influenza A virus in viral replication, transcription and gene expression
Investigator(s):	Poon LLM, Guan Y, Peiris JSM
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	11/2003

Abstract:

N/A

Project Title:	Splicing of influenza A virus
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2004

Abstract:

To study the sequence requirements for the splicing of influenza viral mRNA; to study the role of splicing in controlling viral protein expression; to generate recombinant influenza viruses with mutations in the splicing sequences and determine the global effects of mutations in these regions in an influenza life cycle; to test whether there is a host-specific selective pressure on the 5' splicing site of influenza mRNA.

Project Title:	Splicing of influenza A virus
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

N/A

Project Title:	Membrane fusion of influenza A virus
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2005
Completion Date:	06/2006

Abstract:

N/A

Project Title:	Development of one-step reverse trasncriptase-loop mediated isothermal amplification for direct detections of H5N1 and human influenza viruses
Investigator(s):	Poon LLM
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	04/2006

Abstract:

Introduction A diagnosis of influenza A virus could be made by classical methods such as virus isolation, haemagglutinin inhibition test, antigen detection and serology. In the last decade, the rapid advancement of molecular techniques has opened up a new horizon for clinical diagnosis of influenza virus. Molecular techniques, such as PCR, mutliplex PCR, real-time PCR and nucleic acid sequence-based amplification (NASBA) are shown to be useful for clinical applications. These molecular techniques are demonstrated to be highly sensitive and specific. More importantly, most of the above molecular tests could produce diagnostic results in a very short period of time, thereby making these molecular approaches become attractive tools for rapid diagnosis of influenza viruses. These molecular tests, however, often require sophisticated equipment, expensive reagents and highly skilled personnel. Thus, these rapid molecular tests might not be the method of choice in basic clinical settings in developing countries or in field situations. The recent invention of loop-mediated isothermal amplification (LAMP) has opened up a new horizon for molecular diagnosis (1). Briefly, this method depends on auto-cycling strand displacement DNA synthesis performed by a Bst DNA polymerase. The reaction relies on recognition of the target sequence by six independent sequences in the initial stage and by four independent sequences during the later stages of the LAMP reaction, thereby making this kind of assays to be highly specific. The amplification products are stem-loop DNA structures with several inverted repeats of the target and form cauliflower-like structures with multiple loops. This approach is extreme rapid and has DNA amplification efficiency equivalent to that of PCR-based methods (1). More importantly, this approach is extreme simple and all reactions are performed in an isothermal environment. Furthermore, no special apparatus is needed, which makes it more economical and practical than nested PCR or real-time PCR. These advantages make LAMP become very applicable in very basic healthcare/field settings. Recently, using the LAMP approach, we developed several molecular assays for detecting cDNA from several pathogens (2-4). In these previous studies, the performances of these assays were at least comparable to routine molecular diagnostic tests. More importantly, we successfully used this technology for a direct detection DNA of Plasomodium falciparum in heat-treated blood (5). More importantly, our modifications of the assays could reduce the reagent cost to as low as 2 HK dollars per test (5). We expect the low running cost of this detection approach would be an attractive option for molecular diagnosis. However, our modified technology still cannot be used for a direct detection of RNA species in clinical samples. The recent outbreaks of H5 influenza viruses in Southeast Asia suggest a new pandemic influenza strain will evolve from this geographical region. Based on the experiences from other outbreaks (e.g. SARS), the availabilities of rapid diagnostic tests for H5N1 influenza virus will play a key role in patient management and policy making. We therefore reason that, due to its low running cost, the LAMP assay might be used as a screening test for extensive influenza surveillance/diagnosis. In particular, we expect this assays might be adopted by other developing countries, thereby allowing more extensive H5N1 surveillances in these countries. Here, we propose to develop a one-step reverse transcriptase (RT)-LAMP assay that allows direct detection sof RNA species from clinical samples. Aims In this study, we aim at: 1. developing a general one-step RT-LAMP protocol that allows direct detections of RNA species from clinical samples; 2. developing one-step RT-LAMP assays for avian H5N1 influenza A virus; 3 developing one-step RT-LAMP assays for human influenza A virus. References 1. Notomi et al, 2000. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res. 28:E63. 2. Poon et al, 2004. Rapid detection of the severe acute respiratory syndrome (SARS) coronavirus by a loop-mediated isothermal amplification assay. Clin Chem. 50:1050. 3. Poon et al, 2005. Detection of human influenza A viruses by loop-mediated isothermal amplification. J Clin Microbiol. 43:427. 4. Poon et al, 2005. Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. J Clin Microbiol. 43:3457. 5. Poon et al, A sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification. Clin Chem. In press.

List of Research Outputs

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Chan M.C.W., Cheung C.Y., Chui W.H., Tsao G.S.W., Nicholls J.M., Chan Y.O., Chan W.Y., Long H.T., Poon L.L.M., Guan Y. and Peiris J.S.M., Proinflammatory cytokine responses induced by influenza A (H5N1) viruses in primary human alveolar and bronchial epithelial cells, Respiratory Research. 2005, 6: 135.

Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Chan K.H., Mak W.W., Ngan H.Y.S., Xu X., Screaton G., Tam P.K.H., Austyn J.M., Chan L.C., Yip S.P., Peiris J.S.M., Khoo U.S. and Lin C.L., Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection. , Nature Genetics. 2006, 38: 38-46.

Drosten C., Chan K.H. and Poon L.L.M., Viral diagnosis of SARS, In: Stohr K, Anderson L, Osterhaus A, Yuen KY, Peiris JSM (eds). Severe acute respiratory syndrome. Blackwell, Oxford, 2005, 64-71.

Khoo U.S., Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Ngan H.Y.S., Tam P.K.H., Chan L.C., Yip S.P., Peiris J.S.M., Sham P.C. and Lin C.L., The protective role of homozygous L-SIGN (CLEC4M) in SARS coronavirus infection., HUGO's 11th Human Genome Meeting, Helsinki, Finland. . 2006.

Nicholls J.M., Butany J., Poon L.L.M., Chan K.H., Beh S.L., Poutanen S., Peiris J.S.M. and Wong M.P., Time course and cellular localization of SARS-CoV nucleoprotein and RNA in lungs from fatal cases of SARS, Public Library of Science Medicine . 2006, 3(2): e27.

Peiris J.S.M. and Poon L.L.M., The SARS coronavirus (SARS-CoV)., In: SARS: How a global epidemic was stopped. World Health Organization. Geneva.. Mackenzie, 2006, 210-217.

Peiris J.S.M., Poon L.L.M. and Mackenzie J.S., The SARS coronavirus (SARS-CoV), SARS - How a global epidemic was stopped. World Health Organization, 2006, 210-217.

Poon L.L.M., Editor, International Journal Of Virology (launch In 2006). Academic Journals Inc., 2005.

Poon L.L.M., Wong W.Y., Chan K.H., Ng S.F., Yuen K.Y., Guan Y. and Peiris J.S.M., Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. , Journal of Clinical Microbiology. 2005, 43: 3457-3459.

Poon L.L.M., Influenza surveillance and diagnosis, Symposium in flu research and diagnostic advances, Taipei, Taiwan. 2005.

Poon L.L.M., Influenza surveillance in animals , H5N1 technical meeting in Jakarta, Indonesia. 2006.

Poon L.L.M., Influenza surveillance in animals, Avian influenza and pigs workshop, Bogor, Indonesia. 2005.

Poon L.L.M., Influenza transcription and replication, Frontiers in Biomedical Research, Hong Kong. 2005.

Poon L.L.M., Insluenza RNA Polymerase as target for therapeutics, 3rd Pasteur-Asia Virology Course a training course, Hong Kong. 2006.

Poon L.L.M., Molecular biology of SARS coronaviurs, the 2nd Pasteur-Asia Virology course a training course, Hong Kong. 2005.

Poon L.L.M. and Lo Y.M.D., Non-invasive methods for detecting non-host DNA in a host using epigenetic differences between the host and non-host DNA. US patent number: 6,927,028 , 2005.

Poon L.L.M., Leung C.S.W., Chan K.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates. , Journal of Medical Virology. 2005, 76: 435-40.

Poon L.L.M., Wong W.Y., Ma H.T., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S. and Peiris J.S.M., Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification, Clinical Chemistry. 2006, 52(2): 303-6.

Poon L.L.M., Chan K.H. and Drosten C., Viral diagnosis of SARS, Severe acute respiratory syndrome: a clinical guide.. Blackwell, 2005, 64-71.

Poon L.L.M., WHO training program for avian influenza diagnosis, Bogor, Indonesia. . 2005.

Webster R.G., Guan Y., Poon L.L.M., Krauss S., Webby R., Govorkovai E. and Peiris J.S.M., The spread of the H5N1 bird flu epidemic in Asia in 2004, Archives of Virology Supplementum. 2005, 19: 117-29.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Researcher : Poon RWS

List of Research Outputs

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Researcher : Qin K

List of Research Outputs

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Que TL

List of Research Outputs

Ho P.L., Que T.L., Ng T.K., Chiu S.S.S., Yung R.W.H. and Tsang K.W.T., Clinical outcomes of bacteremic pneumococcal infections in an area with high resistance, European Journal of Clinical Microbiology and Infectious Diseases. 2006, 25(5): 323-7.

Researcher : Rayner JM

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Researcher : Seto WH

List of Research Outputs

Ho P.L., Cheng J.C., Ching P.T.Y., Kwan J.K., Lim W.W.L., Tong W.C., Wu T.C., Tse C.W., Lam R., Yung R.W.H. and Seto W.H., Optimising antimicrobial prescription in hospitals by introducing an antimicrobial stewardship programme in Hong Kong: consensus statement, Hong Kong Medical Journal. 2006, 12(2): 141-8.

Leung T.Y., Ho P.L., Yuen K.Y., Woo W.L., Lam T.H., Kao R.Y.T., Seto W.H. and Yam W.C., Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA, Antimicrobial Agents and Chemotherapy. 2006, 50(3): 1075-8.

Tsang K.W.T., Shim Y.S., Wong T.K., Liam C.K., Eng P., Lam W.K. and Seto W.H., Possible case scenarios and logistic issues in H5N1 pandemic, Respirology. 2006, 11: 520-522.

Yam W.C., Yuen K.Y., Kam S.Y., Yiu L.S., Chan K.S., Leung C.C., Tam C.M., Ho P.O., Yew W.W., Seto W.H. and Ho P.L., Diagnostic application of genotypic identification of mycobacteria, Journal of Medical Microbiology. 2006, 55(Pt 5): 529-36.

Researcher : Shiu SYW

Project Title:	Role of PDZD2 in the modulation of prostate cancer cell proliferation
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2005

Abstract:

To determine the expression of full-length and secreted forms of PDZD2 in common prostate cancer cell lines; to detect any changes in the expression of the full-length and secreted forms of PDZD2 upon hormonal stimulation of prostate cancer cell proliferation; to study the effect of decreased endogenous expression of PDZD2 on prostate cancer cell proliferation.

Project Title:	sPDZD2-induced protein profile changes in prostate cancer
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To express and purify sPDZD2 in E. coli, and to study the effects of the recombinant protein on the protein profiles of human prostate cancer to cells using a 'proteomics' approach.

Project Title:	Functional roles of PDZD2 and sPDZD2 in prostate cancer pathogenesis
Investigator(s):	Shiu SYW
Department:	Physiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2005
Completion Date:	06/2006

Abstract:

N/A

Project Title:	Overexpression of sPDZD2 in prostate cancer cells
Investigator(s):	Shiu SYW, Yao KM
Department:	Physiology
Source(s) of Funding:	Small Project Funding
Start Date:	10/2005

Abstract:

PDZ (PSD95, Discs-large, and ZO-1) domain containing protein 3 (PDZK3), also known as PDZ domain containing protein 2 (PDZD2), is a ubiquitously expressed protein of unknown function which has six PDZ domains. The protein is cleaved in a caspase-dependent mechanism to generate a secreted protein (sPDZD2), which may participate in extracellular signaling [Yeung et al. 2003, EMBO Rep 4: 412]. Interestingly, gene expression of PDZK3 has been reported to be upregulated in prostate cancer when compared with benign tissues Thus, it is believed that PDZK3, also named as activated in prostate cancer protein (AIPC), may play an important role in prostate tumorigenesis [Chaib et al. 2001, Cancer Res 61:2390]. Given that our ongoing studies have demonstrated that sPDZD2 protein is produced and secreted by human prostate cancer cell lines, we would like to postulate that sPDZD2 protein, a cleavage product of PDZD2, may play an important role in prostate cancer pathogenesis by modulating prostate cancer cell growth and differentiation. The objectives of this proposal are to 1) over-express sPDZD2 protein in prostate cancer cells, and 2) to study any effect of the protein on the expression of cell cycle and apoptosis-related proteins in the transfected cancer cells.

List of Research Outputs

Researcher : Smith GJ

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

de Jong M.D., Thanh T.T., Khanh T.T., Hien V.M., Smith G.J., Chau N.V., Cam B.V., Qui P.V., Ha D.Q., Guan Y., Peiris J.S.M., Hien T.T. and Farrar J., Oseltamivir Resistance during Treatment of Influenza A (H5N1) Infection, New England Journal of Medicine. 2005, 353: 2667-2672.

Researcher : Tang SF

List of Research Outputs

Chu C.M., Cheng V.C.C., Hung I.F.N., Chan K.S., Tang S.F., Tsang T.H.F., Chan H.Y. and Yuen K.Y., Viral Load Distribution in SARS Outbreak, Emerging Infectious Diseases. 2005, 11(12): 1882-1886.

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M.Y., Tang S.F. and Yuen K.Y., Clostridium bacteremia characterized by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology. 2005, 58: 301-307.

Researcher : Teng LL

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Li N.K.H., Teng L.L., Leung K.W., Ng K.H.L., Que T.L. and Yuen K.Y., Globicatella bacteremia identified by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology . 2006, 59: 303-307.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, Journal of Clinical Microbiology. 2005, 43: 919-922.

Teng L.L., Laribacter hongkongensis, a novel bacterium associated with gastroenteritis (PhD thesis), supervisor: Woo P.C.Y., Yuen K.Y. , 2005.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C.Y., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous abuser with pseudobacteraemia. , Microbiology and Immunology. 2005, 49: 31-39.

Woo P.C.Y., Teng L.L., Lau S.K.P. and Yuen K.Y., Clinical, phenotypic, and genotypic evidence for Streptococcus sinensis as the common ancestor of anginosus and mitis groups of streptococci, Medical Hypotheses . 2005, 66: 345-351.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Kao R.Y.T., Lau S.K.P. and Yuen K.Y., Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, FEMS Microbiology Letters. 2005, 252: 57-65.

Woo P.C.Y., Lau S.K.P., Teng L.L. and Yuen K.Y., Current status and future directions of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis and traveller's diarrhoea, Current Opinions in Infectious Disease. 2005, 18: 413-419.

Researcher : To KKW

List of Research Outputs

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Researcher : Tsang DNC

List of Research Outputs

Peiris J.S.M., Tsang D.N.C. and Lim W.W.L., Virological diagnosis of SARS, In: Jane CK Chan, Vivian CW Taam Wong, Challenges of Severe Acute Respiratory Syndrome. Saunders Elsevier, 2005, 1: 299-316.

Researcher : Tsang THF

List of Research Outputs

Chu C.M., Cheng V.C.C., Hung I.F.N., Chan K.S., Tang S.F., Tsang T.H.F., Chan H.Y. and Yuen K.Y., Viral Load Distribution in SARS Outbreak, Emerging Infectious Diseases. 2005, 11(12): 1882-1886.

Researcher : Tse H

Project Title:	Phenotypic and genotypic characterization of Helcococcus isolates in Hong Kong and their mechanisms of erythromycin resistance
Investigator(s):	Tse H, Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To perform phenotypic and molecular characterization on the Helcococcus isolates from patients in Hong Kong and investigate its mechanism of erythromycin resistance

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess, Systematic and Applied Microbiology . 2005, 28: 316-322.

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

Woo P.C.Y., Tse H., Wong S.S.Y., Tse C.W., Fung A.M.Y., Tam D.M.W., Lau S.K.P. and Yuen K.Y., Life-threatening invasive Helcococcus kunzii infections in intravenous drug users and ermA-mediated erythromycin resistance., Journal of Clinical Microbiology. 2005, 43: 6205-6208.

Researcher : Tsoi HW

List of Research Outputs

Lau S.K.P., Ho P.L., Li W.S., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and Characterization of Chromosomal Class C -Lactamase and Its Regulatory Gene in Laribacter hongkongensis., Antimicrobial Agents and Chemotherapy. 2005, 49: 1957-1964.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Lau S.K.P., To W.K., Tse P.W.T., Chan A.K.H., Woo P.C.Y., Tsoi H.W., Leung A.F.Y., Li K.S.M., Chan P.K.S., Lim W.W.L., Yung R.W.H., Chan K.H. and Yuen K.Y., Human parainfluenza virus 4 outbreak and the role of diagnostic tests, Journal of Clinical Microbiology. 2005, 43: 4515-4521.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chen Z.W., Wong B.H.L., Chan J.K.H., Wong L.P., He W., Ma C., Chan K.H., Ho D.D. and Yuen K.Y., SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus, Vaccine. 2005, 23: 4959-4968.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Woo P.C.Y., Huang Y., Lau S.K.P., Tsoi H.W. and Yuen K.Y., In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease, Microbiology and Immunology. 2005, 49: 899-908.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Woo P.C.Y., Lau S.K.P., Huang Y., Tsoi H.W., Chan K.H. and Yuen K.Y., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia., Archives of virology. 2005, 150(11): 2299-311.

Researcher : Wang J

List of Research Outputs

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Wang P

List of Research Outputs

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Researcher : Webster RG

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Choi Y.K., Nguyen T.D., Ozaki H., Webby R.J., Puthavathana P., Buranathal C., Chaisingh A., Auewarakul P., Hanh N.T., Ma S.K., Hui P.Y., Guan Y., Peiris J.S.M. and Webster R.G., Studies of H5N1 influenza virus infection of pigs by using viruses isolated in Vietnam and Thailand in 2004., Journal of Virology. 2005, 79(16): 10821-5.

Hulse-Post D.J., Sturm-Ramirez K.M., Humberd J., Seiler P., Govorkova E.A., Krauss S., Scholtissek C., Puthavathana P., Buranathai C., Nguyen T.D., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Role of domestic ducks in the propagation and biological evolution of highly pathogenic H5N1 influenza viruses in Asia., Proceedings of the National Academy of Sciences of the United States of America. 2005, 102(30): 10682-7.

Sturm-Ramirez K.M., Hulse-Post D.J., Govorkova E.A., Humberd J., Seiler P., Puthavathana P., Buranathai C., Nguyen T.D., Chaisingh A., Long H.T., Naipospos T.S., Chen H., Ellis T.M., Guan Y., Peiris J.S.M. and Webster R.G., Are ducks contributing to the endemicity of highly pathogenic H5N1 influenza virus in Asia?, Journal of Virology. 2005, 79(17): 11269-79.

Webster R.G., Peiris J.S.M., Chen H. and Guan Y., H5N1 outbreaks and enzootic influenza, Emerging Infectious Diseases. 2006, 12(1): 3-8.

Webster R.G., Guan Y., Poon L.L.M., Krauss S., Webby R., Govorkovai E. and Peiris J.S.M., The spread of the H5N1 bird flu epidemic in Asia in 2004, Archives of Virology Supplementum. 2005, 19: 117-29.

Researcher : Wong BHL

List of Research Outputs

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chen Z.W., Wong B.H.L., Chan J.K.H., Wong L.P., He W., Ma C., Chan K.H., Ho D.D. and Yuen K.Y., SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus, Vaccine. 2005, 23: 4959-4968.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Researcher : Wong CW

List of Research Outputs

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

Researcher : Wong GKM

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Researcher : Wong HLB

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Researcher : Wong KH

List of Research Outputs

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., Development of New Technique for Detection of HIV Specific Cytotoxic T Lymphocytes, Keystone Symposia HIV Vaccines (×6). 27 March-2 April. 2006.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Zhang H. and Lu L., Ex vivo expansion of functional CD4 T cells from HIV patients., The 6th International Workshop on HIV, Cells of Macrophage/Dendritic Lineage and Other Reservoirs.. 2005, 37.

Researcher : Wong KL

List of Research Outputs

He M.L., Zheng B., Chen Y., Wong K.L., Huang J., Lin M.C., Peng Y., Yuen K.Y., Sung J.J. and Kung H.F., Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus., FEBS Letters. 2006, 580(10): 2414-2420.

Researcher : Wong LP

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chen Z.W., Wong B.H.L., Chan J.K.H., Wong L.P., He W., Ma C., Chan K.H., Ho D.D. and Yuen K.Y., SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus, Vaccine. 2005, 23: 4959-4968.

Researcher : Wong MKM

List of Research Outputs

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess, Systematic and Applied Microbiology . 2005, 28: 316-322.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C.Y., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous abuser with pseudobacteraemia. , Microbiology and Immunology. 2005, 49: 31-39.

Researcher : Wong SSY

Project Title:	Molecular characterization of streptococcus bovis bacteraemia
Investigator(s):	Wong SSY, Yuen KY, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To characterize 48 S. bovis strains isolated from the blood cultures of 37 bacteraemic patients over a six-year period using a combination of phenotypic and genotypic techniques. The epidemiology, underlying diseases, clinical disease associations, and outcome of S. bovis bacteraemia in relation to the different biotypes and genotypes of S. bovis will be investigated, and the molecular epidemiology of erythromycin resistance in the S. bovis strains will also be analyzed.

Project Title:	Screening antigenic proteins in penicillium marneffei for seroepidemiology and vaccination
Investigator(s):	Wong SSY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

To screen for yeast- and mould-phase-specific antigens that are useful for epidemiological and diagnostic purposes; to screen for yeast-phase- and mould-phase-specific antigens that induce active immunity against penicilliosis marneffei from normalized cDNA libraries of the two phases of the dimorphic fungus; to construct a prophylactic and/or therapeutic vaccine prototype; to study the seroepidemiology of penicilliosis marneffei.

Project Title:	Screening antigenic proteins in penicillium marneffei for seroepidemiology and vaccination
Investigator(s):	Wong SSY, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

N/A

Project Title:	Characterization of bacterial isolates with ambiguous biochemical profiles recovered from patients with clinically significant bacteremia by 16S rRNA gene sequencing, the state-of-the-art molecular technique for bacterial identification
Investigator(s):	Wong SSY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To use the state-of-the-art molecular technique for bacterial identification, 16S rRNA gene sequencing, to characterize these bacterial isolates. Novel bacterial species discovered will be further characterized.

Project Title:	Phenotypic and genotypic characterization of Streptococcus iniae isolates in Hong Kong
Investigator(s):	Wong SSY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To perform phenotypic and molecular characterization on the Streptococcus iniae isolates from patients in Hong Kong.

List of Research Outputs

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

Lau S.K.P., Woo P.C.Y., Luk W.K., Fung A.M.Y., Hui W.T., Fong A.H.C., Chow C.W., Wong S.S.Y. and Yuen K.Y., Clinical isolates of Streptococcus iniae from Asia are more mucoid and beta-hemolytic than those from North America., Diagnostic Microbiology and Infectious Diseases. 2006, 54: 177-181.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Mak C.K., Ho P.L., Tse C.W., Lau S.K.P. and Wong S.S.Y., Reduced levofloxacin susceptibility and tetracycline resistance in a clinical isolate of Haemophilus quentini identified by 16S rRNA sequencing., Journal of Clinical Microbiology. 2005, 43: 5391-5392.

Wong S.S.Y. and Yuen K.Y., Avian influenza virus infections in human, Chest. 2006, 129: 156-168.

Wong S.S.Y. and Yuen K.Y., Influenza vaccination: options and issues., Hong Kong Medical Journal. 2005, 11: 381-390.

Wong S.S.Y. and Yuen K.Y., Nasopharyngeal detection of severe acute respiratory syndrome-associated coronavirus RNA in health-care workers., Chest. 2006, 129: 12-13.

Wong S.S.Y. and Yuen K.Y., The severe acute respiratory syndrome (SARS)., Journal of NeuroVirology. 2005, 11: 455-468.

Wong S.S.Y. and Yuen K.Y., Zoonotic potential of emerging animal diseases, BMJ (Clinical research ed.). 2005, 331(7527): 1256-1260.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Tse H., Wong S.S.Y., Tse C.W., Fung A.M.Y., Tam D.M.W., Lau S.K.P. and Yuen K.Y., Life-threatening invasive Helcococcus kunzii infections in intravenous drug users and ermA-mediated erythromycin resistance., Journal of Clinical Microbiology. 2005, 43: 6205-6208.

Woo P.C.Y., Lau S.K.P., Lau C.Y., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient, Bone Marrow Transplantation. 2005, 35: 831-833.

Researcher : Wong WY

List of Research Outputs

Poon L.L.M., Wong W.Y., Chan K.H., Ng S.F., Yuen K.Y., Guan Y. and Peiris J.S.M., Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. , Journal of Clinical Microbiology. 2005, 43: 3457-3459.

Poon L.L.M., Wong W.Y., Ma H.T., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S. and Peiris J.S.M., Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification, Clinical Chemistry. 2006, 52(2): 303-6.

Researcher : Woo GKS

List of Research Outputs

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Lau S.K.P., Woo P.C.Y., Chan C.Y.F., Woo W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi, Journal of Clinical Microbiology. 2005, 43: 1470-1472.

Lau S.K.P., Ng K.H.L., Woo P.C.Y., Yip K.T., Fung A.M.Y., Woo G.K.S., Chan K.M., Que T.L. and Yuen K.Y., Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures, Journal of Clinical Pathology. 2006, 59: 219-222.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess, Systematic and Applied Microbiology . 2005, 28: 316-322.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C.Y., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous abuser with pseudobacteraemia. , Microbiology and Immunology. 2005, 49: 31-39.

Researcher : Woo PCY

Project Title:	Molecular epidemiology of erythromycin resistant [beta]-hemolytic Lancefield group G streptococci causing bacteremia
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To perform phenotypic and genotypic characterization of the erythromycin resistance in [beta]-hemolytic group G streptococci recovered from blood cultures of patients in a six-year period. Risk factors for bacteremia caused by [beta]-hemolytic group G streptocci will also be analyzed.

Project Title:	The origin of antibiotic resistance genes: a genome based phylogentic analysis
Investigator(s):	Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004
Completion Date:	10/2006

Abstract:

To perform a genome based in silico analysis to determine the reason for antibiotic resistance genes in actinomycetes being the "origins" of some antibiotic resistance genes.

Project Title:	The role of a novel bacterium, Laribacter hongkongensis, in gastroenteritis, and its epidemiology and pathogenesis
Investigator(s):	Woo PCY
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

N/A

Project Title:	The role of a novel bacterium, Laribacter hongkongensis, in gastroenteritis, and its epidemiology and pathogenesis
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To study the association of L. hongkongensis with gastroenteritis in humans and the epidemiology of this novel disease entity; to provide clinicians with appropriate treatment regimen by correlation of antibiotic susceptibilities and clinical outcome; to discover the source and reservoir of the bacterium; to define its pathogenesis in terms of toxin production, tissue and animal invasiveness.

Project Title:	Complete genome sequencing and characterization of the urease gene cassette of Laribacter hongkongensis, a novel bacterium associated with freshwater fish related community-acquired gastroenteritis and traveler's diarrhea
Investigator(s):	Woo PCY
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2005
Completion Date:	06/2006

Abstract:

N/A

Project Title:	Usefulness of full sequence and 5' end 527-bp 16S ribosomal RNA gene sequencing for identification of medically important anaerobic bacteria
Investigator(s):	Woo PCY, Lau SKP, Yuen KY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To study usefulness of full sequence and 5' end 527-bp 16S ribosomal RNA gene sequencing for identification of medically important anaerobic bacteria.

Project Title:	A prospective study on the epidemiology of coronavirus-associated respiratory tract infections in Hong Kong and the role of rapid disgnostic tests
Investigator(s):	Woo PCY, Yuen KY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

The present study will generate valuable information for understanding the prevalence, the clinical spectrum of disease and epidemiology of the different coronaviruses in all age groups, and in turn their public health significance and economic implications, as compared to other respiratory viral pathogens. Rapid tests useful for the detection of the various coronaviruses from clinical specimens will also be developed.

List of Research Outputs

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Lau S.K.P., Woo P.C.Y., Luk W.K., Fung A.M.Y., Hui W.T., Fong A.H.C., Chow C.W., Wong S.S.Y. and Yuen K.Y., Clinical isolates of Streptococcus iniae from Asia are more mucoid and beta-hemolytic than those from North America., Diagnostic Microbiology and Infectious Diseases. 2006, 54: 177-181.

Lau S.K.P., Ho P.L., Li W.S., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and Characterization of Chromosomal Class C -Lactamase and Its Regulatory Gene in Laribacter hongkongensis., Antimicrobial Agents and Chemotherapy. 2005, 49: 1957-1964.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Lau S.K.P., Woo P.C.Y., Li N.K.H., Teng L.L., Leung K.W., Ng K.H.L., Que T.L. and Yuen K.Y., Globicatella bacteremia identified by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology . 2006, 59: 303-307.

Lau S.K.P., To W.K., Tse P.W.T., Chan A.K.H., Woo P.C.Y., Tsoi H.W., Leung A.F.Y., Li K.S.M., Chan P.K.S., Lim W.W.L., Yung R.W.H., Chan K.H. and Yuen K.Y., Human parainfluenza virus 4 outbreak and the role of diagnostic tests, Journal of Clinical Microbiology. 2005, 43: 4515-4521.

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Lau S.K.P., Woo P.C.Y., Chan C.Y.F., Woo W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi, Journal of Clinical Microbiology. 2005, 43: 1470-1472.

Lau S.K.P., Ng K.H.L., Woo P.C.Y., Yip K.T., Fung A.M.Y., Woo G.K.S., Chan K.M., Que T.L. and Yuen K.Y., Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures, Journal of Clinical Pathology. 2006, 59: 219-222.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, Journal of Clinical Microbiology. 2005, 43: 919-922.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chen Z.W., Wong B.H.L., Chan J.K.H., Wong L.P., He W., Ma C., Chan K.H., Ho D.D. and Yuen K.Y., SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus, Vaccine. 2005, 23: 4959-4968.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess, Systematic and Applied Microbiology . 2005, 28: 316-322.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C.Y., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous abuser with pseudobacteraemia. , Microbiology and Immunology. 2005, 49: 31-39.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Biliary tract disease is a risk factor for Plesiomonas shigelloides bacteremia: a nine-year experience in a Hong Kong hospital and review of the literature, New Microbiologica . 2005, 28: 45-55.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Teng L.L., Lau S.K.P. and Yuen K.Y., Clinical, phenotypic, and genotypic evidence for Streptococcus sinensis as the common ancestor of anginosus and mitis groups of streptococci, Medical Hypotheses . 2005, 66: 345-351.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M.Y., Tang S.F. and Yuen K.Y., Clostridium bacteremia characterized by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology. 2005, 58: 301-307.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Kao R.Y.T., Lau S.K.P. and Yuen K.Y., Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, FEMS Microbiology Letters. 2005, 252: 57-65.

Woo P.C.Y., Control of the H5N1 influenza epidemic at the source, HGM 2006 meeting, Helsinki, Finland. 2006.

Woo P.C.Y., Lau S.K.P., Teng L.L. and Yuen K.Y., Current status and future directions of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis and traveller's diarrhoea, Current Opinions in Infectious Disease. 2005, 18: 413-419.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., Genomic evidence for antibiotic resistance genes of actinomycetes as origins of antibiotic resistance genes in pathogenic bacteria simply because actinomycetes are more ancestral than pathogenic bacteria, Medical Hypotheses. 2006, 67: 1297-1304.

Woo P.C.Y., Huang Y., Lau S.K.P., Tsoi H.W. and Yuen K.Y., In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease, Microbiology and Immunology. 2005, 49: 899-908.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Infectious diseases emerging from Chinese wet-markets: zoonotic origins of severe respiratory viral infections, Current Opinion in Infectious Diseases. 2006, 19: 401-407.

Woo P.C.Y., Tse H., Wong S.S.Y., Tse C.W., Fung A.M.Y., Tam D.M.W., Lau S.K.P. and Yuen K.Y., Life-threatening invasive Helcococcus kunzii infections in intravenous drug users and ermA-mediated erythromycin resistance., Journal of Clinical Microbiology. 2005, 43: 6205-6208.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Woo P.C.Y., Lau S.K.P., Lau C.Y., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient, Bone Marrow Transplantation. 2005, 35: 831-833.

Woo P.C.Y., Lau S.K.P., Huang Y., Tsoi H.W., Chan K.H. and Yuen K.Y., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia., Archives of virology. 2005, 150(11): 2299-311.

Researcher : Wu AKL

List of Research Outputs

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Researcher : Wu WL

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Xia N

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Xu K

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Yam WC

List of Research Outputs

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

Ha W.Y., Lau C.C., Yue P.Y., Hung K.K., Chan K., Chui S.H., Chui A.K., Yam W.C. and Wong R.N., Simultaneous detection of precore/basal core promoter mutations in hepatitis B virus using arrayed primer extension, Mol Diagn Ther. 2006, 10(2): 125-134.

Lam R.C.W., Leung M.K.H., Leung Y.C., Vrijmoed L.L.P., Yam W.C., Ng S.P. and Chu P.K., Photocatalytic glass reactor for solar-assisted air purification, Proceedings of the 2005 Solar World Congress. Orlando, Florida, USA, 2005, CD-ROM.

Leung M.K.H., Liu C.H., Chan A.H.S., Leung Y.C., Yam W.C., Ng S.P. and Vrijmoed L.L.P., Prediction of transient turbulent dispersion by CFD-statistical hybrid modeling method, Atmospheric Environment. Oxford, UK, Elsevier Ltd., 2005, 39: 6345-6351.

Leung T.Y., Ho P.L., Yuen K.Y., Woo W.L., Lam T.H., Kao R.Y.T., Seto W.H. and Yam W.C., Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA, Antimicrobial Agents and Chemotherapy. 2006, 50(3): 1075-8.

Ma E.S.K., Wong C.L., Lai K.T., Chan E.C., Yam W.C., Chan A.C. and Chan A.C.Y., Kocuria kristinae infection associated with acute cholecystitis. , BMC Infect Dis. UK, 2005, 5(1): 60.

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Yam W.C., Yuen K.Y., Kam S.Y., Yiu L.S., Chan K.S., Leung C.C., Tam C.M., Ho P.O., Yew W.W., Seto W.H. and Ho P.L., Diagnostic application of genotypic identification of mycobacteria, Journal of Medical Microbiology. 2006, 55(Pt 5): 529-36.

Researcher : Yew WW

List of Research Outputs

Cheng V.C.C., Yew W.W. and Yuen K.Y., Molecular diagnostics in tuberculosis., European journal of clinical microbiology & infectious diseases. 2005, 24(11): 711-720.

Researcher : Yip CK

List of Research Outputs

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Researcher : Yip CY

List of Research Outputs

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Researcher : Yuen KY

Project Title:	Establishment of core facilities for the HKU HIV/AIDS research laboratories
Investigator(s):	Yuen KY, Zheng B, Woo PCY, Yam WC, Im SWK
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	11/2001

Abstract:

To support the development of state-of-art clinical management of HIV/AIDS founded on scientific research and clinical trials; to undertake research for expanding the knowledge base on HIV molecular epidemiology in Hong Kong and the reigon; to examine the immunological mechanisms underlying HIV pathogenesis; to study AIDS associated infectious diseases; to build expertise in HIV molecular biology and immunology.

Project Title:	Molecular characterization of Streptococcus milleri causing infective endocarditis
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To identify members of the "S. milleri group" in patients with infective endocarditis by 16S rRNA gene sequencing; to define the epidemiology, clinical spectrum, treatment, and outcome of infective endocarditis associated with the "S. milleri group".

Project Title:	An oral mucosal DNA vaccine for SARS coronavirus infections
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	09/2004

Abstract:

To test the feasibility of using the live-attenuated Salmonella system for delivery of DNA vaccine of the spike protein of SARS-CoV in mice, and compare the immunogenicity of oral DNA vaccine, naked DNA vaccine, and recombinant protein vaccine.

Project Title:	Characterization of a novel bacterial genus and species, Anaerospora hongkongensis gen. nov.sp.nov., isolated from the blood culture of a patient
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004
Completion Date:	10/2006

Abstract:

To characterize a novel bacterial genus and species using various phenotypic and genotypic techniques; to understad its evolutional relationship to other related bacterial genus and species.

Project Title:	Characterisation of local/emerging pathogens and development of anti-infective agents
Investigator(s):	Yuen KY, Jin LJ, Kao RYT, Lau G, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Seed Funding for Strategic Research Theme
Start Date:	05/2005

Abstract:

There are 4 categories of pathogens are selected to compete with the rest of the world and therefore advance the research of our University to top international level: hepatitis B virus (HBV), Laribacter Hongkongenesis, influenze A virus and novel oral pathogens.

Project Title:	The role of a novel coronavirus, coronavirus HKU1, in respiratory illness and its epidemiology and molecular evolution
Investigator(s):	Yuen KY
Department:	Microbiology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	07/2005

Abstract:

N/A

Project Title:	The role of a novel coronavirus, coronavirus HKU1, in respiratory illness and its epidemiology and molecular evolution
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	07/2005

Abstract:

To study the role of CoV-HKU1 in respiratory illness in humans and its clinical spectrum of disease and epidemiology; to develop laboratory tests for the diagnosis of CoV-HKU1 infection; to study the molecular evolution of this novel coronavirus.

Project Title:	Bacteraemia caused by Anaerotruncus colihominis and emended description of the species
Investigator(s):	Yuen KY, Lau SKP, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Small Project Funding
Start Date:	09/2005

Abstract:

To characterize a strain of Anaerotruncus colihominis recovered from the blood culture of a patient in Hong Kong and provide an emended description of the species.

Project Title:	Wild animal surveillance for coronavirus HKU1, a novel coronavirus associated with pneumonia in patients in Hong Kong, and potential variants of other coronaviruses that infect human
Investigator(s):	Yuen KY, Lau SKP, Woo PCY
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	12/2005

Abstract:

The present study is going to yield valuable information for understanding the reservoir of coronavirus HKU1 and potential variants of other coroanviruses that infect human, which is crucial for the control of respiratory tract infections associated with the viruses.

Project Title:	A multilocus sequence typing system for Penicillum marneffei: an international molecular cyber system for tracking the origin and transmission of Penicillium marneffei
Investigator(s):	Yuen KY, Woo PCY, Lau SKP
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2006

Abstract:

In this project, using the sequence data of the Penicillium marneffei Genome Project, we propose to build up a multilocus sequence typing (MLST) system, for this fungus.

List of Research Outputs

Au W.Y., Cheng T.S., Siu T.S. and Yuen K.Y., Cerebellar degeneration and folate deficiency due to cough misture abuse, Haematologica. 2005, 90:(ECR)28: Epub.

Au W.Y., Kwong Y.L. and Yuen K.Y., Hemophagocytosis in the peripheral blood due to tuberculosis mycobacteremia, American Journal of Medicine. 2005, 118(11): 1298-9.

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Poon L.L.M., Guan Y., Seto W.H., Yuen K.Y. and Peiris J.S.M., Serological response in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63, Clinical and Diagnostic Laboratory Immunology. 2005, 12: 1317-1321.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Cheng V.C.C., Yew W.W. and Yuen K.Y., Molecular diagnostics in tuberculosis., European journal of clinical microbiology & infectious diseases. 2005, 24(11): 711-720.

Cheng V.C.C., Ho P.L. and Yuen K.Y., Two probable cases of serious drug interaction between clarithromycin and colchicine., The Southern medical journal. 2005, 98(8): 811-3.

Cheng V.C.C., Ho P.L. and Yuen K.Y., Two probable cases of serious drug interaction between clarithromycin and colchicine, South Medical Journal. 2005, 98(8): 811-3.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Chu C.M., Cheng V.C.C., Hung I.F.N., Chan K.S., Tang S.F., Tsang T.H.F., Chan H.Y. and Yuen K.Y., Viral Load Distribution in SARS Outbreak, Emerging Infectious Diseases. 2005, 11(12): 1882-1886.

Duan R.S., Sit T.H., Wong S.S.Y., Wong C.W., Chow K.H., Mak C.K., Yam W.C., Ng L.T., Yuen K.Y. and Ho P.L., Escherichia coli producing CTX-M beta-lactamases in food animals in Hong Kong, Microbial Drug Resistance. 2006, 12(2): 145-8.

He M.L., Zheng B., Chen Y., Wong K.L., Huang J., Lin M.C., Peng Y., Yuen K.Y., Sung J.J. and Kung H.F., Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus., FEBS Letters. 2006, 580(10): 2414-2420.

Hung I.F.N., Wu A.K.L., Cheng V.C.C., Tang S.F., To K.K.W., Yeung C.K., Woo P.C.Y., Lau S.K.P., Cheung B.M.Y. and Yuen K.Y., Fatal interaction between clarithromycin and colchicine in patients with renal insufficiency: a retrospective study., Clinical infectious diseases. 2005, 41(3): 291-300.

Lau S.K.P., Woo P.C.Y., Luk W.K., Fung A.M.Y., Hui W.T., Fong A.H.C., Chow C.W., Wong S.S.Y. and Yuen K.Y., Clinical isolates of Streptococcus iniae from Asia are more mucoid and beta-hemolytic than those from North America., Diagnostic Microbiology and Infectious Diseases. 2006, 54: 177-181.

Lau S.K.P., Ho P.L., Li W.S., Tsoi H.W., Yung R.W.H., Woo P.C.Y. and Yuen K.Y., Cloning and Characterization of Chromosomal Class C -Lactamase and Its Regulatory Gene in Laribacter hongkongensis., Antimicrobial Agents and Chemotherapy. 2005, 49: 1957-1964.

Lau S.K.P., Woo P.C.Y., Yip C.Y., Tse H., Tsoi H.W., Cheng V.C.C., Lee P., Tang S.F., Cheung C.H.Y., Lee R.A., So L.Y., Lau Y.L., Chan K.H. and Yuen K.Y., Coronavirus HKU1 and Other Coronavirus Infections in Hong Kong, Journal of Clinical Microbiology. 2006, 44(6): 2063-2071.

Lau S.K.P., Woo P.C.Y., Li N.K.H., Teng L.L., Leung K.W., Ng K.H.L., Que T.L. and Yuen K.Y., Globicatella bacteremia identified by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology . 2006, 59: 303-307.

Lau S.K.P., To W.K., Tse P.W.T., Chan A.K.H., Woo P.C.Y., Tsoi H.W., Leung A.F.Y., Li K.S.M., Chan P.K.S., Lim W.W.L., Yung R.W.H., Chan K.H. and Yuen K.Y., Human parainfluenza virus 4 outbreak and the role of diagnostic tests, Journal of Clinical Microbiology. 2005, 43: 4515-4521.

Lau S.K.P., Che X.Y., Woo P.C.Y., Wong B.H.L., Cheng V.C.C., Woo G.K.S., Hung I.F.N., Poon R.W.S., Chan K.H., Peiris J.S.M. and Yuen K.Y., SARS coronavirus detection methods., Emerging infectious diseases. 2005, 11(7): 1108-11.

Lau S.K.P., Woo P.C.Y., Li S.M.K., Huang Y., Tsoi H.W., Wong H.L.B., Wong S.S.Y., Leung S.Y., Chan K.H. and Yuen K.Y., Severe acute respiratory syndrome coronavirus-like virus in Chinese horseshoe bats., Proceedings of the National Academy of Sciences of the Unite States of America. 2005, 102: 14040–14045.

Lau S.K.P., Woo P.C.Y., Chan C.Y.F., Woo W.L., Woo G.K.S. and Yuen K.Y., Typhoid fever associated with acute appendicitis caused by an H1-j strain of Salmonella enterica serotype Typhi, Journal of Clinical Microbiology. 2005, 43: 1470-1472.

Lau S.K.P., Ng K.H.L., Woo P.C.Y., Yip K.T., Fung A.M.Y., Woo G.K.S., Chan K.M., Que T.L. and Yuen K.Y., Usefulness of the MicroSeq 500 16S rDNA bacterial identification system for identification of anaerobic Gram positive bacilli isolated from blood cultures, Journal of Clinical Pathology. 2006, 59: 219-222.

Leung A.Y.H., Yuen K.Y. and Kwong Y.L., Polyma BK virus and haemorrhagic cystitis in haematopoietic stem cell transplantation - a changing paradigm, Bone Marrow Transplantation. 2005.

Leung A.Y.H., Yuen K.Y. and Kwong Y.L., Polyoma BK virus and haemorrhagic cystitis in haematopoietic stem cell transplantation: a changing paradigm, Bone Marrow Transplantation. 2005, 36(11): 929-37.

Leung A.Y.H., Wong A.S.Y., Chan G.C.F., Yuen K.Y. and Kwong Y.L., Urinary reactivation of polyoma BK virus but not adenovirus in paediatric and adolescent patients after haematopoietic stem cell transplantation, Bone Marrow Transplantation. 2006, 37(6): 621-3.

Leung T.Y., Ho P.L., Yuen K.Y., Woo W.L., Lam T.H., Kao R.Y.T., Seto W.H. and Yam W.C., Molecular characterization of isoniazid resistance in Mycobacterium tuberculosis: identification of a novel mutation in inhA, Antimicrobial Agents and Chemotherapy. 2006, 50(3): 1075-8.

Peiris J.S.M., Anderson L.J., Osterhaus A.D.M.E., Stohr K. and Yuen K.Y., Editor, "Severe Acute Respiratory Syndrome". Blackwell Publishing, 2005, 1: 1-263.

Peiris J.S.M. and Yuen K.Y., Influenza, In: Mary Ip, Moira Chan-Yeung, Wah Kit Lam, Nan Shan Zhong, Respiratory Medicine: An Asian Perspective. Hong Kong University Press, 2005, 1: 209-221.

Poon L.L.M., Wong W.Y., Chan K.H., Ng S.F., Yuen K.Y., Guan Y. and Peiris J.S.M., Evaluation of real-time reverse transcriptase PCR and real-time loop-mediated amplification assays for severe acute respiratory syndrome coronavirus detection. , Journal of Clinical Microbiology. 2005, 43: 3457-3459.

Poon L.L.M., Leung C.S.W., Chan K.H., Yuen K.Y., Guan Y. and Peiris J.S.M., Recurrent mutations associated with isolation and passage of SARS coronavirus in cells from non-human primates. , Journal of Medical Virology. 2005, 76: 435-40.

Poon L.L.M., Wong W.Y., Ma H.T., Chan K.H., Chow L.M., Abeyewickreme W., Tangpukdee N., Yuen K.Y., Guan Y., Looareesuwan S. and Peiris J.S.M., Sensitive and inexpensive molecular test for falciparum malaria: detecting Plasmodium falciparum DNA directly from heat-treated blood by loop-mediated isothermal amplification, Clinical Chemistry. 2006, 52(2): 303-6.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of Huh7 cell line by SARS coronavirus and human coronavirus 229E., Journal of Virology. 2005, 79: 6180-6193.

Teng L.L., Woo P.C.Y., Ma S.S.L., Sit T.H.C., Ng L.T., Lau S.K.P. and Yuen K.Y., Ecoepidemiology of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, Journal of Clinical Microbiology. 2005, 43: 919-922.

Wong S.S.Y. and Yuen K.Y., Avian influenza virus infections in human, Chest. 2006, 129: 156-168.

Wong S.S.Y. and Yuen K.Y., Influenza vaccination: options and issues., Hong Kong Medical Journal. 2005, 11: 381-390.

Wong S.S.Y. and Yuen K.Y., Nasopharyngeal detection of severe acute respiratory syndrome-associated coronavirus RNA in health-care workers., Chest. 2006, 129: 12-13.

Wong S.S.Y. and Yuen K.Y., The severe acute respiratory syndrome (SARS)., Journal of NeuroVirology. 2005, 11: 455-468.

Wong S.S.Y. and Yuen K.Y., Zoonotic potential of emerging animal diseases, BMJ (Clinical research ed.). 2005, 331(7527): 1256-1260.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Chen Z.W., Wong B.H.L., Chan J.K.H., Wong L.P., He W., Ma C., Chan K.H., Ho D.D. and Yuen K.Y., SARS coronavirus spike polypeptide DNA vaccine priming with recombinant spike polypeptide from Escherichia coli as booster induces high titer of neutralizing antibody against SARS coronavirus, Vaccine. 2005, 23: 4959-4968.

Woo P.C.Y., Tse H., Lau S.K.P., Leung K.W., Woo G.K.S., Wong M.K.M., Ho C.M. and Yuen K.Y., Alkanindiges hongkongensis sp. nov. A novel Alkanindiges species isolated from a patient with parotid abscess, Systematic and Applied Microbiology . 2005, 28: 316-322.

Woo P.C.Y., Teng L.L., Leung K.W., Lau S.K.P., Woo G.K.S., Wong A.C.Y., Wong M.K.M. and Yuen K.Y., Anaerospora hongkongensis gen. nov. sp. nov., a novel genus and species with ribosomal DNA operon heterogeneity isolated from an intravenous abuser with pseudobacteraemia. , Microbiology and Immunology. 2005, 49: 31-39.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Biliary tract disease is a risk factor for Plesiomonas shigelloides bacteremia: a nine-year experience in a Hong Kong hospital and review of the literature, New Microbiologica . 2005, 28: 45-55.

Woo P.C.Y., Lau S.K.P., Chu C.M., Chan K.H., Tsoi H.W., Huang Y., Wong B.H.L., Poon R.W.S., Cai J., Luk W.K., Poon L.L.M., Wong S.S.Y., Guan Y., Peiris J.S.M. and Yuen K.Y., Characterisation and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia, Journal of Virology. 2005, 79: 884-895.

Woo P.C.Y., Lau S.K.P., Tsoi H.W., Poon R.W.S., Chu C.M., Lee R.A., Luk W.K., Wong G.K.M., Wong B.H.L., Cheng V.C.C., Tang S.F., Wu A.K.L., Yung R.W.H., Chen H., Guan Y., Chan K.H. and Yuen K.Y., Clinical and molecular epidemiological features of coronavirus HKU1-associated community-acquired pneumonia, Journal of Infectious Diseases . 2005, 192: 1898-1907.

Woo P.C.Y., Teng L.L., Lau S.K.P. and Yuen K.Y., Clinical, phenotypic, and genotypic evidence for Streptococcus sinensis as the common ancestor of anginosus and mitis groups of streptococci, Medical Hypotheses . 2005, 66: 345-351.

Woo P.C.Y., Lau S.K.P., Chan K.M., Fung A.M.Y., Tang S.F. and Yuen K.Y., Clostridium bacteremia characterized by 16S ribosomal RNA gene sequencing, Journal of Clinical Pathology. 2005, 58: 301-307.

Woo P.C.Y., Ma S.S.L., Teng L.L., Li W.S., Kao R.Y.T., Lau S.K.P. and Yuen K.Y., Construction of an inducible expression shuttle vector for Laribacter hongkongensis, a novel bacterium associated with gastroenteritis, FEMS Microbiology Letters. 2005, 252: 57-65.

Woo P.C.Y., Lau S.K.P., Teng L.L. and Yuen K.Y., Current status and future directions of Laribacter hongkongensis, a novel bacterium associated with gastroenteritis and traveller's diarrhoea, Current Opinions in Infectious Disease. 2005, 18: 413-419.

Woo P.C.Y., Lau S.K.P., Wong B.H.L., Tsoi H.W., Fung A.M., Kao R.Y.T., Chan K.H., Peiris J.S.M. and Yuen K.Y., Differential sensitivities of severe acute respiratory syndrome (SARS) coronavirus spike polypeptide enzyme-linked immunosorbent assay (ELISA) and SARS coronavirus nucleocapsid protein ELISA for serodiagnosis of SARS coronavirus pneumonia., Journal of Clinical Microbiology. 2005, 43(7): 3054-8.

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

Woo P.C.Y., Lau S.K.P., Huang Y. and Yuen K.Y., Genomic evidence for antibiotic resistance genes of actinomycetes as origins of antibiotic resistance genes in pathogenic bacteria simply because actinomycetes are more ancestral than pathogenic bacteria, Medical Hypotheses. 2006, 67: 1297-1304.

Woo P.C.Y., Huang Y., Lau S.K.P., Tsoi H.W. and Yuen K.Y., In silico analysis of ORF1ab in coronavirus HKU1 genome reveals a unique putative cleavage site of coronavirus HKU1 3C-like protease, Microbiology and Immunology. 2005, 49: 899-908.

Woo P.C.Y., Lau S.K.P. and Yuen K.Y., Infectious diseases emerging from Chinese wet-markets: zoonotic origins of severe respiratory viral infections, Current Opinion in Infectious Diseases. 2006, 19: 401-407.

Woo P.C.Y., Tse H., Wong S.S.Y., Tse C.W., Fung A.M.Y., Tam D.M.W., Lau S.K.P. and Yuen K.Y., Life-threatening invasive Helcococcus kunzii infections in intravenous drug users and ermA-mediated erythromycin resistance., Journal of Clinical Microbiology. 2005, 43: 6205-6208.

Woo P.C.Y., Lau S.K.P., Li K.S.M., Poon R.W.S., Wong B.H.L., Tsoi H.W., Yip C.K., Huang Y., Chan K.H. and Yuen K.Y., Molecular diversity of coronaviruses in bats, Virology. 2006, 351: 180-187.

Woo P.C.Y., Lau S.K.P., Lau C.Y., Chong T.K., Hui W.T., Wong S.S.Y. and Yuen K.Y., Penicillium marneffei fungaemia in an allogeneic bone marrow transplant recipient, Bone Marrow Transplantation. 2005, 35: 831-833.

Woo P.C.Y., Lau S.K.P., Huang Y., Tsoi H.W., Chan K.H. and Yuen K.Y., Phylogenetic and recombination analysis of coronavirus HKU1, a novel coronavirus from patients with pneumonia., Archives of virology. 2005, 150(11): 2299-311.

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Yam W.C., Yuen K.Y., Kam S.Y., Yiu L.S., Chan K.S., Leung C.C., Tam C.M., Ho P.O., Yew W.W., Seto W.H. and Ho P.L., Diagnostic application of genotypic identification of mycobacteria, Journal of Medical Microbiology. 2006, 55(Pt 5): 529-36.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., Development of New Technique for Detection of HIV Specific Cytotoxic T Lymphocytes, Keystone Symposia HIV Vaccines (×6). 27 March-2 April. 2006.

Researcher : Yung RWH

List of Research Outputs

Ho P.L., Que T.L., Ng T.K., Chiu S.S.S., Yung R.W.H. and Tsang K.W.T., Clinical outcomes of bacteremic pneumococcal infections in an area with high resistance, European Journal of Clinical Microbiology and Infectious Diseases. 2006, 25(5): 323-7.

Ho P.L., Cheng J.C., Ching P.T.Y., Kwan J.K., Lim W.W.L., Tong W.C., Wu T.C., Tse C.W., Lam R., Yung R.W.H. and Seto W.H., Optimising antimicrobial prescription in hospitals by introducing an antimicrobial stewardship programme in Hong Kong: consensus statement, Hong Kong Medical Journal. 2006, 12(2): 141-8.

Researcher : Zhang J

List of Research Outputs

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Cheung C.L., Rayner J.M., Smith G.J., Wang P., Naipospos T.S., Zhang J., Yuen K.Y., Webster R.G., Peiris J.S.M., Guan Y. and Chen H., Distribution of amantadine-resistant H5N1 avian influenza variants in Asia., The Journal of infectious diseases. 2006, 193(12): 1626-1629.

Researcher : Zhang L

List of Research Outputs

Butt K.M., Smith G.J., Chen H., Zhang L., Leung C.Y.H., Xu K., Lim W.W.L., Webster R.G., Yuen K.Y., Peiris J.S.M. and Guan Y., Human infection with an avian H9N2 influenza A virus in Hong Kong in 2003., Journal of clinical microbiology. 2005, 43(11): 5760-5767.

Chen H., Smith G.J., Li K.S., Wang J., Fan X., Rayner J.M., Dhanasekaran V., Zhang J., Zhang L., Guo C., Cheung C.L., Xu K., Duan L., Huang K., Qin K., Leung C.Y.H., Wu W.L., Lu H., Chen Y., Xia N., Naipospos T.S., Yuen K.Y., Hassan S.S., Bahri S., Nguyen T.D., Webster R.G., Peiris J.S.M. and Guan Y., Establishment of multiple sublineages of H5N1 influenza virus in Asia: implications for pandemic control., Proceedings of the National Academy of Sciences of the United States of America. 2006, 103(8): 2845-2850.

Researcher : Zhao X

List of Research Outputs

Zheng B., Duan Z.P., Zhao X., Huang D.Z., He L.X., Chen Y. and Zhao C.H., RANTES gene single nucleotide polymorphisms and expression in patients with chronic hepatitis B virus infection., International Meeting on the Molecular Biology of Hepatitis B Viruses. 18-21 September 2005. Heidelberg, Germany.. 2005.

Researcher : Zheng B

Project Title:	CCR5 expression on antigen-presenting cens in HIV infection
Investigator(s):	Zheng B, Lee SS
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	01/2001

Abstract:

To describe the pattern of CCR5 expression on APC in HIV infection; to correlate this pattern to HIV infected APC; to determine the functional and/or quantitative deficiency in CCR5 expressing APC.

Project Title:	The pattern of chemokine receptor polymorphism in healthy and HIV positive Chinese adults
Investigator(s):	Zheng B, Lee SS
Department:	Microbiology
Source(s) of Funding:	Council for the AIDS Trust Fund - General Award
Start Date:	01/2001

Abstract:

To determine the prevalence of the major forms of CCR5 promoter and CCR2 polymorphism in healthy Chinese adults; to compare the pattern between healthy adults and HIV infected individuals in Hong Kong.

Project Title:	Studies of SARS virus vaccines
Investigator(s):	Zheng B, Guan Y
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2004
Completion Date:	01/2006

Abstract:

To develop SCoV vaccines for prevention of SARS; to develop an inactivated vaccine and a cold attenuated live vaccine.

Project Title:	Studies of SARS virus vaccines
Investigator(s):	Zheng B, Guan Y
Department:	Microbiology
Source(s) of Funding:	Research Fund for the Control of Infectious Diseases - Full Grants
Start Date:	01/2005

Abstract:

The study objectives are developments of an inactivated vaccine and a cold attenuated live vaccine.

Project Title:	A mechanism study of spike protein in the infection of SARS coronavirus
Investigator(s):	Zheng B
Department:	Microbiology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2005
Completion Date:	06/2006

Abstract:

N/A

Project Title:	A preliminary study on the inhibition of avian infuenza virus H5N1 replication using siRNA
Investigator(s):	Zheng B
Department:	Microbiology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2006
Completion Date:	12/2006

Abstract:

1. Study purposes Searching for effective and specific antiviral agents against H5N1 avian flu is one of the world-wide top priorities in influenza research. Our project aims to assess the feasibility of using RNAi to inhibit the replication of H5N1 virus in cell cultures. 2. Key issues (1) The first outbreak of H5N1 avian flu in Hong Kong in 1997 and its re-emergence in Southeast Asian since 2004 have afflicted >125 patients and claimed >60 lives. Although the number of patients was limited and there was no evidence that the virus can efficiently transmitted from human to human, the recent outbreaks have suggested that the virus has the potential to trigger a pandemic. (2) The pathology of H5N1 avian flu is poorly understood. To day, no effective and specific treatment for this highly pathogenic disease has been recommended yet. Therefore, development of effective therapeutics and specific vaccination to combat H5N1 avian flu has become a worldwide effort. The high pathogenicity of H5N1 still causes problems and safety concerns for vaccine development. (3) RNAi is effective for inhibition of viral replication. However, it is not known whether the highly pathogenic H5N1 may have evolved to express anti-RNAi proteins or RNAs. Our and others’ pilot studies have demonstrated that siRNAs targeting PA, NP and M2 genes of H5N1 virus can effectively inhibit viral replication. The current proposal is a logical extension of these studies. (4) The roles of the other 3 structural (H, N & M1) and 5 non-structural (PB2, PB1, NS1 & NS2) genes of H5N1 in viral replication are distinct. Hence, siRNAs targeting different H5N1 genes may have synergistic effects, thereby avoiding the rapid emergence of siRNA-resistsant mutants. (5) Delivery of RNAi into live animals and humans is a challenging task and has been under intense investigation. Yet, many questions concerning the efficacy, tissue specificity and safety of delivery systems remain unanswered. The development and optimization of effective delivery systems is the key barrier to siRNA therapeutics. In this regard, adenovirus (Ad) and adenovirus associated virus (AAV) vectors may have some advantages for delivery of siRNAs against influenza viruses into mice and humans. 3. Project objectives (1) Rational design and evalution of additional siRNAs targeting multiple structural and nonstructural genes of H5N1 virus in cultured cells. (2) Analysis of the synergistic antiviral effects of different siRNAs obtained. (3) Development of effective non-viral and viral based systems for delivering H5N1-targeting siRNAs.

List of Research Outputs

Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Chan K.H., Mak W.W., Ngan H.Y.S., Xu X., Screaton G., Tam P.K.H., Austyn J.M., Chan L.C., Yip S.P., Peiris J.S.M., Khoo U.S. and Lin C.L., Homozygous L-SIGN (CLEC4M) plays a protective role in SARS coronavirus infection. , Nature Genetics. 2006, 38: 38-46.

He M.L., Zheng B., Chen Y., Wong K.L., Huang J., Lin M.C., Peng Y., Yuen K.Y., Sung J.J. and Kung H.F., Kinetics and synergistic effects of siRNAs targeting structural and replicase genes of SARS-associated coronavirus., FEBS Letters. 2006, 580(10): 2414-2420.

Khoo U.S., Chan V.S.F., Chan Y.K., Chen Y., Poon L.L.M., Cheung A.N.Y., Zheng B., Ngan H.Y.S., Tam P.K.H., Chan L.C., Yip S.P., Peiris J.S.M., Sham P.C. and Lin C.L., The protective role of homozygous L-SIGN (CLEC4M) in SARS coronavirus infection., HUGO's 11th Human Genome Meeting, Helsinki, Finland. . 2006.

Li B.J., Tang Q., Cheng D., Qin C., Xie F.Y., Wei Q., Xu J., Liu Y., Zheng B., Martin M.C., Zhong N.S. and Lu P.Y., Using siRNA in prophylactic and therapeutic regimens against SARS coronavirus in Rhesus macaque, Nature Medicine. 2005, 11: 944-951.

Wang M., Yan M.Y., Xu H.F., Liang W.L., Kan B., Zheng B., Chen H., Zheng H., Xu Y.M., Zhang E.M., Wang H.X., Ye J.R., Li G.C., Li M.C., Cui Z.G., Liu Y.F., Guo R.T., Liu X.N., Zhan D.H., Zhou D.H., Zhao A.L., Hai R., Yu D.Z., Guan Y. and Xu J.G., SARS-CoV Infection In A Restaurant From Palm Civet., Emerg Infect Dis. 2005, 11: 1860-1865.

Yam W.C., Chen J.H.K., Wong K.H., Chan K.C.W., Cheng V.C.C., Lam H.Y., Lee S.S., Zheng B. and Yuen K.Y., Clinical utility of genotyping resistance test on determining the mutation patterns in HIV-1 CRF01_AE and subtype B patients receiving antiretroviral therapy in Hong Kong., Journal of Clinical Virology. 2006, 35: 454-457.

Zhang M., Ko K.H., Lam Q.L.K., Srivastava G., Zheng B., Lau Y.L. and Lu L., Expression and function of TNF family member B cell-activating factor in the development of autoimmune arthritis, International Immunology. 2005, 17(8): 1081-1092.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Chan C.S., Ng F., Huang F. and Yuen K.Y., Development of New Technique for Detection of HIV Specific Cytotoxic T Lymphocytes, Keystone Symposia HIV Vaccines (×6). 27 March-2 April. 2006.

Zheng B., Lee S.S., Wong K.H., Chan K.C.W., Zhang H. and Lu L., Ex vivo expansion of functional CD4 T cells from HIV patients., The 6th International Workshop on HIV, Cells of Macrophage/Dendritic Lineage and Other Reservoirs.. 2005, 37.

Zheng B., Duan Z.P., Zhao X., Huang D.Z., He L.X., Chen Y. and Zhao C.H., RANTES gene single nucleotide polymorphisms and expression in patients with chronic hepatitis B virus infection., International Meeting on the Molecular Biology of Hepatitis B Viruses. 18-21 September 2005. Heidelberg, Germany.. 2005.

Zheng B., Cao K., Chan C.P.Y., Duan Z.H., Gao Y., Wang M., Di B., Hollidt J.M., Bergmann A., Lehmann M., Renneberg I., Tam J.S.L., Chan P.K.S., Cautherley G.W.H., Fuchs D. and Renneberg R., Serum Neopterin for Early Assessment of Severity of Severe Acute Respiratory Syndrome, Clin Immunol. 2005, 16: 18-26.

Zheng Y., Cao K., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H., Lu L. and Zheng B., Complementary activation of peripheral natural killer cell immunity in nasopharyngeal carcinoma, Cancer Science. 2006, 97(9): 912-9.

Researcher : Zheng Y

List of Research Outputs

Chen H., Smith G.J., Zheng Y., Qin K., Wang J., Li K.S., Webster R.G., Peiris J.S.M. and Guan Y., Avian flu: H5N1 virus outbreak in migratory waterfowl., Nature. 2005, 436(7048): 191-2.

Zheng Y., Cao K., Ng S.P., Chua D.T.T., Sham J.S.T., Kwong D.L.W., Ng M.H., Lu L. and Zheng B., Complementary activation of peripheral natural killer cell immunity in nasopharyngeal carcinoma, Cancer Science. 2006, 97(9): 912-9.

Researcher : Zhou C

List of Research Outputs

Woo P.C.Y., Chong T.K., Tse H., Cai J., Lau C.Y., Zhou C., Lau S.K.P. and Yuen K.Y., Genomic and experimental evidence for a potential sexual cycle in the pathogenic thermal dimorphic fungus Penicillium marneffei., FEBS letters. 2006, 580(14): 3409-3416.

-- End of Listing --