Dept of Pathology

DEPARTMENT OF PATHOLOGY

Researcher : Beh SL

Project Title:	The attitudes of doctors toward rape victims
Investigator(s):	Beh SL
Department:	Pathology
Source(s) of Funding:	Other Funding Scheme
Start Date:	06/1993

Abstract:

To have an objective assessment of what the prevalent attitudes are; to ascertain if there is a gender bias; to compare local data with data from other countries; to compare data with other professional groups.

Project Title:	Homicide in three Chinese cities: Hong Kong, Shenzhen and Beijing
Investigator(s):	Beh SL
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	10/2000
Completion Date:	12/2004

Abstract:

To examine the nature of homicide in Hong Kong, Shenzhen and Beijing. The project aims to provide a medico-criminological dimension often lacking in homicide studies.

Project Title:	A study of the profile of causes of death in patients suspected to suffer from the Severe Acute Respiratory Syndrome (SARS) and its impact on subsequent clinical management
Investigator(s):	Beh SL, Wong MP, Nicholls JM, Chung LP, Lee K.C., Pang S.W., Leung C.Y.
Department:	Pathology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To analyze the profile of the causes of death in patients with suspected SARS.

List of Research Outputs

Aggrawal A., Busutil A., Baccinol E., Clark J., Beh S.L., Clement J. .G., Cordner S., Karch S., Ernst M.F. and Maden A., Editorial Advisory Board, In: Jason Payne-James, Roger W. Byard, Tracey S. Cprey, Carol Henderson, Encyclopedia of Forensic and Legal Medicine. Oxford, Elsevier, 2005, 1-4.

Beh S.L. and Broadhurst R.G., Child Homicide in Hong Kong 1989-1998, American Academy of Forensic Sciences Annual Conference. 2005.

Beh S.L. and Luo B., Death Investigation Systems: China, In: Jason Payne-James, Roger W. Byard, Tracey S. Corey, Carol Henderson, Encyclopedia of Forensic and Legal Medicine. Oxford, Elsevier Academic Press, 2005, 2: 120-122.

Beh S.L., Deaths: Trauma, Musculoskeletal System , In: Jason Payne-James, Roger W Byard, Tracey S Corey 7 Carol Henderson, Encyclopedia of Forensic and Legal Medicine . Oxford, UK, Elsevier Academic Press, 2005, 2: 103-106.

Beh S.L., Sexual Offenses, Adult: Global Crime Figures and Statistics, In: Jason Payne-James, Roger W. Byard, Tracey S. Corey, Carol Henderson, Encyclopedia of Forensic and Legal Medicine. Oxford, Elsevier Academic Press, 2005, 4: 111-116.

Gibson I., Cheung L.K., Chow S.P., Cheung W.L., Beh S.L., Savalani M.M. and Lee S.H., The use of rapid prototyping to assist medical applications, Proceedings of the 10th Assises Europoeennes du Prototypage Rapide. Paris, France, AFPR, 2004, CD-ROM: 1-8.

Researcher : Chan ASW

List of Research Outputs

Wong C.W., Fan Y.S., Chan T.L., Chan A.S.W., Ho L.C., Ma T.K.F., The Cancer Genome Project , Yuen S.T. and Leung S.Y., BRAF and NRAS mutations are uncommon in melanomas arising in diverse internal organs, Journal of Clinical Pathology. 2005, 58: 640-644.

Researcher : Chan ASY

List of Research Outputs

Chan L.C., Chan A.S.Y., Hui E.C., Ha S.Y. and Ma E.S.K., Hb H Disease: Time to Screen?, Genetics and Population Health. Inaugural Conference of the Australian Public Health Genetics Consortium. 2004.

Leung S.Y., Yuen S.T., Chu K.M., Mathy J.A., Li R., Chan A.S.Y., Law S.Y.K., Wong J., Chen X. and So S., Expression profiling identifies chemokine (C-C motif) ligand 18 as an independent prognostic indicator in gastric cancer, Gastroenterology. 2004, 127(2): 457-469.

Researcher : Chan CF

List of Research Outputs

Chan C.F., NF-kappa B pathway dysregulation and relation to HBx in hepatocellular carcinoma, Master of Philosophy thesis. 2004.

Researcher : Chan CY

List of Research Outputs

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Frequent down-regulation of HDPR1, a novel inhibitor of WNT/beta-catenin signaling, in hepatocellular carcinoma (Abstract), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of WNT/b-catenin signaling, is down-regulated in hepatocellular carcinoma: involvement of promoter hypermethylation, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of the WNT/beta-catenin signaling, is frequently downregulated in hepatocellular carcinoma: involvement of methylation-mediated gene silencing, Oncogene. 2005, 24(9): 1607-1614.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), Asian Journal of Surgery. 2005, 28(Suppl 2): S84.

Researcher : Chan DW

Project Title:	Characterization of the roles of Hepatitis B virus X protein in hepatocellular carcinoma
Investigator(s):	Chan DW, Ng IOL
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To knock down the expression of HBx in an HBx-expressing HCC cell line (PLC/PRF/5), (RNAi); to investigate the expression levels of HBx-induced oncogenes in PLC/PRF/5 cells with knock-down of HBx; to evaluate the change of tumorigenicity of PLC/PRF/5 cells with knock-down of HBx using in vitro and in vivo assays.

List of Research Outputs

Chan D.W., Lee M.F., Chan P.C.Y. and Ng I.O.L., T-cadherin is silenced by frequent genetic and epigenetic alterations in hepatocellular carcinoma, The 11th HK International Cancer Congress, 10-12 November 2004, Hong Kong. 2004.

Liu V.W.S., Chiu P.M., Yao K.M., Chan D.W., Hui C.C., Cheung A.N.Y. and Ngan H.Y.S., Study of hedgehog signaling in cervical cancer, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Researcher : Chan EYT

List of Research Outputs

Ip E.W.K., Chan K.H., Law H.K.W., Tso H.W., Kong K.P., Wong W.H.S., To Y.F., Yung W.W.H., Chow E.Y., Au K.L., Chan E.Y.T., Lim W., Jensenius J.C., Turner M.W., Peiris J.S.M. and Lau Y.L., Mannose-Binding Lectin in Severe Acute Respiratory Syndrome Coronavirus Infection, Journal of Infectious Diseases. 2005, 191: 1697-1704.

Mok T.M.Y., Chan E.Y.T., Lo Y., Wong R.W.S. and Lau W.C.S., Antiphospholipid (aPL) antibody profiles in Chinese patients with systemic lupus erythematosus, 11th APLAR Bi-annual Congress, Jeju, Korea 11-16 September 2004.

Mok T.M.Y., Chan E.Y.T., Fong D.Y.T., Leung K.F.S., Wong W.S. and Lau W.C.S., Antiphospholipid Antibody Profiles and Their Clinical Associations in Chinese Patients with Systemic Lupus Erythematosus, The Journal of Rheumatology. 2005, 32 (4): 622-8.

Ng M.W., Law H.K.W., Yung R.W.H., Chow E.Y., Au K.L., Chan E.Y.T., Lim W., Peiris J.S.M. and Lau Y.L., Association of Monocyte Chemoattractant Protein 1 Gene Polymorphism with Severe Acute Respiratory Syndrome (SARS), Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 51.

Researcher : Chan GSW

List of Research Outputs

Chan G.S.W., Lam M.F., Au W.Y., Tse K.C., Chan D.T.M., Lai K.N. and Chan K.W., IgA nephropathy complicating graft-versus-host disease, another nephropathy causing nephrotic syndrome after bone marrow transplantation, Histopathology. 2004, 45(6): 648-651.

Chan G.S.W. and Chan K.W., Images in pathology: adventures of pinocchio, International Journal of Surgical Pathology. 2005, 13: 205.

Chan G.S.W., Ng W.K., Nicholls J.M. and Chan K.W., Pathologic quiz case: acute renal failure secondary to an uncommon urinary bladder tumor. Micropapillary transitional cell carcinoma of urinary bladder, Archives of Pathology and Laboratory Medicine. 2005, 129(2): e53–e54.

Researcher : Chan KL

List of Research Outputs

Chan K.L., Guan X.Y. and Ng I.O.L., High-throughput tissue microarray analysis of c-myc activation in chronic liver diseases and hepatocellular carcinoma, Human Pathology. 2004, 35(11): 1324-1331.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Frequent down-regulation of HDPR1, a novel inhibitor of WNT/beta-catenin signaling, in hepatocellular carcinoma (Abstract), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of WNT/b-catenin signaling, is down-regulated in hepatocellular carcinoma: involvement of promoter hypermethylation, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of the WNT/beta-catenin signaling, is frequently downregulated in hepatocellular carcinoma: involvement of methylation-mediated gene silencing, Oncogene. 2005, 24(9): 1607-1614.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), Asian Journal of Surgery. 2005, 28(Suppl 2): S84.

Yau T.O., Chan P.C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), The 4th International Meeting on Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, 14-16 December 2004.

Researcher : Chan KL

List of Research Outputs

Researcher : Chan KW

Project Title:	Proteomic analysis of nasal NK-cell lymphoma: understanding the oncogenic process and in search of novel tumor markers
Investigator(s):	Chan KW
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To understand the proteomic aberrations involved in the malignant transformation of normal NK cell to NL, since alterations in protein expression underlie neoplastic behavior, this proteomic analysis will provide insights into the molecular pathogenesis of NL; to identify tumor markers of NL and develop assessment methods of these tumor markers.

List of Research Outputs

Chan G.S.W., Lam M.F., Au W.Y., Tse K.C., Chan D.T.M., Lai K.N. and Chan K.W., IgA nephropathy complicating graft-versus-host disease, another nephropathy causing nephrotic syndrome after bone marrow transplantation, Histopathology. 2004, 45(6): 648-651.

Chan G.S.W. and Chan K.W., Images in pathology: adventures of pinocchio, International Journal of Surgical Pathology. 2005, 13: 205.

Chan G.S.W., Ng W.K., Nicholls J.M. and Chan K.W., Pathologic quiz case: acute renal failure secondary to an uncommon urinary bladder tumor. Micropapillary transitional cell carcinoma of urinary bladder, Archives of Pathology and Laboratory Medicine. 2005, 129(2): e53–e54.

Chim J.C.S., Wong S.S.Y., Lam C.C.K. and Chan K.W., Concurrent hyperreactive malarial splenomegaly and quartan malarial nephropathy – Plasmodium malariae revisited, Haematologica. 2004, 89(7): ECR21.

Chim S., Lee T.L. and Chan K.W., Propylthiouracil-induced ANCA Positive Crescentic Glomerulonephritis: A Case Report and Literature Review, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 46.

Chim S., Lee T.L. and Chan K.W., Propylthiouracil-induced anca positive crescentic glomerulonephritis: a case report and literature review, The 13th Congress of the International Pediatric Nephrology Association, Adelaide, Australia, 29 August - 2 September 2004.

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Kwok W.K., Ling M.T., Lee D.T.W., Lau T.C.M., Zhou C., Zhang X., Chua C.W., Chan K.W., Chan F.L., Glackin C., Wong Y.C. and Wang X., Up-regulation of TWIST in prostate cancer and its implication as a therapeutic target, Cancer Research. 2005, 65(12): 5153-5162.

Leung J.C.K., Chan Y.Y., Li F.K., Tang S.C.W., Chan K.W., Chan D.T.M., Lam M.F., Wieslander A. and Lai K.N., Glucose degradation products downregulate ZO-1 expression in human peritoneal mesothelial cells: the role of VEGF, Nephrology Dialysis Transplantation. 2005, 20(7): 1336-1349.

Lo C.C., Wong K.Y. and Chan K.W., BRAF mutation in papillary thyroid carcinoma, 13th Annual Scientific Meeting of the Hong Kong Division of the International Academy of Pathology, Hong Kong, 12-14 November 2004.

Lo C.C., Wong K.Y. and Chan K.W., Ras mutation in papillary thyroid carcinoma, 13th Annual Scientific Meeting of the Hong Kong Division of the International Academy of Pathology, Hong Kong, 12-14 November 2004.

Lo S.H., Wong K.S., Arlt V.M., Phillips D.H., Lai C.K., Poon W.T., Chan C.K., Mo K.L., Chan K.W. and Chan A., Detection of Herba Aristolochia Mollissemae in a patient with unexplained nephropathy, American Journal of Kidney Diseases. 2005, 45(2): 407-410.

Ma L., Chan K.W., Trendell-Smith N.J., Lo C.K., To K.W. and Huang F., Necrotic cells induce systemic autoimmune disease in vivo by activation of dendritic cells, 62th Annual Meeting, American Academy of Allergy, Asthma & Immunology (AAAAI), San Antonio, FL, USA, 3-7 March 2005.

Tang S.C.W., Tse K.C., Chan K.W., Ho Y.W. and Lai K.N., Anderson-Fabry disease, American Journal of Kidney Diseases. 2005, 45: A51, e21-22.

Researcher : Chan LC

Project Title:	Mouse models of human leukaemia
Investigator(s):	Chan LC, Sham MH
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2002

Abstract:

The project attempts to understand the role of mixed lineage leukaemia gene (MLL) and EEN in th epathogenesis of leukaemia. Researchers will create mutant mice bearing the MLL-EEN fusion gene by gene targeting in embryonic stem (ES) cells. A conditional knock-in approach will be used in which MLL-EEN fusion gene will be expressed only in cells of the myeloid lineage after birth.

Project Title:	Subcellular localization and functional analysis of EEN/SH3GL1, a protein involved in leukaemia
Investigator(s):	Chan LC, Jin D, Poon R.Y.C.
Department:	Pathology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To sublocalise EEN/SH3GL1 in haemopoietic cells and leukaemias; to determine the regulatory roles of EEN/SH3GL1 in the cell cycle through, (a) microinjection of anti-EEN/SH3GL1 antibody and (b) suppression of EEN/SH3GL1 expression by RNA interference; to study the interaction of EEN/SH3GL1 2 with cyclins and cdks; to investigate whether EEN/SH3GL 1 is a substrate for phosphorylation by cdc2 and if so, to determine the role of the phosphorylated form of EEN/SH3GL 1 during cell cycle.

Project Title:	Ras signaling in human leukaemia
Investigator(s):	Chan LC, Kong CT, So E.C.W.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To study: (1) Identification of the possible interaction between other MLL fusion partners and components of Ras-mediated pathways. i) design of MLL fusion partner constructs. ii) analysis of transactivation of E1K-1, a target of Ras pathway signaling. iii)soft agar transformation assay. (2) Molecular dissection of the potential functional link between Ras-signaling and MLL-AF6 fusion mediated leukaemogenesis using the ML-1 cell line. i) molecular analysis of Ras signaling pathway following suppression of MLL-AF6 expression in ML-1 cell line . ii) effects of inhibition of Ras signaling pathway on cellular growth and apotosis of ML-1 cell line . (3) Validation of the pathogenic significance of Ras-signaling pathway in MLL fusion mediated leukaemogenesis using an Mll-Een knock-in model. i) effects on suppression of Ras signaling pathway on growth and differentiation of embryonic bodies and haemopoiesis colonies derived from Mll-Een targeted ES cells.

Project Title:	ATM mutations in childhood leukaemia
Investigator(s):	Chan LC, Ha SY, Mizutani S., Greaves M.F., Chen S.J.
Department:	Pathology
Source(s) of Funding:	Michael Kadoorie Cancer Genetics Research Fund
Start Date:	01/2005

Abstract:

To screen for ATM gene mutations in childhood ALL samples from Hong Kong, Shanghai, Tokyo and London, UK; to determine the germline status of the ATM gene in patients whose diagnostic samples showed mutations; to determine the presence of ATM mutations or allelic variations in the normal population of samples.

Project Title:	Ras signaling in human leukaemia
Investigator(s):	Chan LC
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Ambekar C.S., Lee J.S.K., Cheung B.M.Y., Chan L.C., Liang R.H.S. and Kumana C.R., Metabolism of Chloramphenicol Succinate, a Competitive Substrate and Inhibitor of Succinate Dehydrogenase: Possible Reason for its Toxicity, Toxicology in Vitro. 2004, 18: 441-7.

Chan L.C., Biological significance of fusion genes in leukaemia, 13th T.B. Teoh Foundation Lecture, Hong Kong College of Pathologists. Hong Kong.. 2004.

Chan L.C., Creating a mouse model of human leukaemia: gene targeting of MLL-EEN fusion gene., Joint seminar of Riken Centre for Development Biology (CDB) and The University of Hong Kong. Kobe, Japan.. 2005.

Chan L.C., Demonstrations on integrated curriculum and PBL teaching, West China School of Medicine of the Sichuan University. Sichuan, China.. 2004.

Chan L.C., Development of Haematology as a Laboratory/Medical Specialty in Hong Kong – Problems & Prospects , Xth Congress of the International Society of Hematology, Asian-Pacific Division, 1-4 September 2004, Nagoya, Japan. 2004.

Chan L.C., From patient to mouse - what we can learn from an animal model of leukaemia., Pediatric Haemat-Oncology Symposium; Centro Hospital Conde de S. Januario. Macau.. 2005.

Chan L.C., Fusion genes in acute leukaemia. , West China School of Medicine of the Sichuan University. Sichuan, China.. 2004.

Chan L.C., Chan A.S.Y., Hui E.C., Ha S.Y. and Ma E.S.K., Hb H Disease: Time to Screen?, Genetics and Population Health. Inaugural Conference of the Australian Public Health Genetics Consortium. 2004.

Chan L.C., Molecular genetics and impact on clinical management. Lessons from childhood leukaemia., Symposium - Molecular Biology: ABC. 11th Hong Kong International Cancer Congress. Hong Kong.. 2004.

Chan L.C., Tea leaves and tails from Hong Kong., 21st Anniversary Celebration. Leukemia Research Fund Centre for Cell & Molecular Biology, ICR, London. . 2005.

Chan L.C., The impact of molecular genetics on the practise of clinical haematology., National Health Care Group (NHG) Annual Scientific Congress. Singapore.. 2004.

Chan L.C., Kong C.T. and Sham M.H., The leukaemia fusion gene, Mll-Een affects haemopoietic development in mouse embryonic stem cells with enhanced proliferation and self renewal of myeloid progenitors, Days of Molecular Medicine 2005 – Stem Cell Biology and Human Disease, 17-19 March 2005, Salk Institute, La Jolla, California, USA. 2005.

Chan L.C., The leukaemia fusion gene, Mll-Een, leads to enhanced proliferation and self renewal of myeloid progenitors in mouse embryonic stem cells, The Croucher Advanced Study Institute on “Molecular Genetics Cell Signaling in Cancers” Hong Kong, 17-21 January 2005.

Chen J.F., Ma E.S.K., Ha S.Y., Chan G.C.F., Chan A.Y.Y., Chan L.C. and Lau Y.L., The Effect of Mild b-Thalassaemia Mutations on Clinical Manifestation of b-Thalassaemia Major and Intermedia in Chinese, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 18.

Chen J.F., Ma E.S.K., Ha S.Y., Chan G.C.F., Chan A.Y.Y., Chan L.C. and Lau Y.L., The Effect of Mild b-thalassaemia Mutations on Clinical Mainfestation of b-thalassaemia Major and Intermedia in Chinese, 5th Guangdong - Hong Kong Paediatric Exchange Meeting, HK, 24 July 2004. 2004.

Cheung N., So C.W., Yam J.W.P., So C.K.C., Poon R.Y.C., Jin D. and Chan L.C., Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukemia, Biochemical Journal. 2004, 383(Pt 1): 27-35.

Ding C., Chiu R.W.K., Lau T.K., Leung T.N., Chan L.C., Chan A.Y.Y., Charoenkwan P., Ng I.S.L., Law H.Y., Ma E.S.K., Xu X., Wanapirak C., Sanguansermsri T., Liao C., Ai M.A.T.J., Chui D.H.K., Cantor C.R. and Lo Y.M.D., MS analysis of single-nucleotide differences in circulating nucleic acids: Application to noninvasive prenatal diagnosis, Proceedings of the National Academy of Sciences. 2004, 101(29): 10762-10767.

Khoo U.S., Chan Y.K., Peiris J.S.M., Yip S.P., Liu W., Ngan H.Y.S., Tam P.K.H., Chan L.C. and Cheung A.N.Y., Association of ICAM3 genetic variant with Severe Acute Respiratory Syndrome (SARS)., HUGO's 10th Human Genome Meeting, 18-21 April 2005, Kyoto, Japan. 2005.

Lu L., Zhang M., Cheung C.T., Wong C., Ko K.H., Tsang S.L., Chan L.C. and Sham M.H., Hoxb3 deficiency impairs B lymphopoiesis, 12th International Conference for Immunology, Montreal, Canada, July 18-23, 2004. 2004.

Ma E.S.K., Wan T.S.K. and Chan L.C., FISHing - Current status and future prospects for improved management of leukemia, Cancer Reviews: Asia-Pacific. 2004, 2(2): 131-141.

Sun Q., Huang F. and Chan L.C., Dendritic cells differentiation from cells of leukemia origin, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Wan T.S.K., Ma E.S.K., Chan G.C.F. and Chan L.C., Investigation of MYCN status in neuroblastoma by fluorescence in situ hybridization, International Journal of Molecular Medicine. 2004, 14: 981-987.

Wan T.S.K., Ma E.S.K., Chow E.Y.D., Li Y.H., Lin S.Y. and Chan L.C., Pathogenesis of jumping translocations: a molecular cytogenetics study, Leukemia Research. 2004, 28(10): 1075-1079.

Yam J.W.P., Jin D. and Chan L.C., Identification and characterization of EBP, a Novel EEN binding protein that inhibits ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.

Researcher : Chan PCY

List of Research Outputs

Chan D.W., Lee M.F., Chan P.C.Y. and Ng I.O.L., T-cadherin is silenced by frequent genetic and epigenetic alterations in hepatocellular carcinoma, The 11th HK International Cancer Congress, 10-12 November 2004, Hong Kong. 2004.

Yau T.O., Chan P.C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), The 4th International Meeting on Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, 14-16 December 2004.

Researcher : Chan QKY

List of Research Outputs

Chan Q.K.Y., Khoo U.S., Chan Y.K., Ngan H.Y.S., Li S.S., Chiu P.M., Man M.L.S., Ip P.P.C., Xue W. and Cheung A.N.Y., Promoter methylation and differential expression of p-Class glutathione S-transferase in endometrial carcinoma, The Journal of Molecular Diagnostics. 2005, 7(1): 8-16.

Chan Q.K.Y., Khoo U.S., Ngan H.Y.S., Yang C., Xue W., Chan Y.K., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Single nucleotide polymorphism of Pi-Class glutathione S-transferase and susceptibility to endometrial carcinoma, Clinical Cancer Research. 2005, 11(8): 2981-2985.

Cheung A.N.Y., Chiu P.M., Chan Q.K.Y., Chan Y.K. and Ngan H.Y.S., Stem cell related genes and malignant progression in gestational trophoblastic diseases, In: 105943, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Researcher : Chan SY

List of Research Outputs

Liu W., Chan Y.K., Chan S.Y., Yip S.P., Cheung A.N.Y., Chua D.T.T., Ngan H.Y.S. and Khoo U.S., BRCA1 gene promoter polymorphisms associated with breast cancer risk, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Researcher : Chan TL

Project Title:	Allele-specific imbalance in gene expression as a cause for hereditary colorectal cancer
Investigator(s):	Chan TL, Leung SY, Yuen ST
Department:	Pathology
Source(s) of Funding:	Michael Kadoorie Cancer Genetics Research Fund
Start Date:	01/2005

Abstract:

To look for allele-specific imbalance in gene expression of the Adenomatous Polyposis Coli (APC), MSH2 and MLH1 genes in FAP or HNPCC patients with undetectable germline mutation; to confirm the pathological significance of the allele with a lower gene expression level by examination for co-segregation with disease and loss of the wild-type allele in cancer tissue; to look for mechanisms that can account for the reduced gene expression level in the disease allele.

Project Title:	Molecular characterisation of the serrated neoplasia pathway and its role in the development of colorectal cancer with mismatch repair deficiency
Investigator(s):	Chan TL, Leung SY, Yuen ST
Department:	Pathology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005

Abstract:

Colorectal cancer (CRC) is one of the commonest cancers world-wide with 850,000 new cases each year. Majority of CRC are known to develop through an adenoma-carcinoma sequence, with molecular genetic changes that characterize each transition step. Adenoma is considered pre-malignant and early prevention and treatment of CRC is possible through regular endoscopic surveillance and removal of tumour at the adenoma stage. However, despite regular surveillance, some patients still develop CRC. One of the possibilities is that there exists another pathway of tumour development. Recently, there are data to suggest the existence of an alternative route, the serrated neoplasia pathway, for CRC development. This latter pathway includes those serrated polyps (SP) that span a morphological spectrum from hyperplastic polyp (HP) to serrated adenoma (SA). The earliest member in this pathway, HP, is a very common lesion in aged individuals and it is a long held belief that HPs are innocent with little propensity for malignant progression. However, a small subset of HPs may progress but the criteria to distinguish the high risk versus the low risk ones are unclear. Concurrently, it is known that a subset (15%) of sporadic CRC manifest a form of genetic instability called microsatellite instability (MSI). This is due to promoter methylation leading to loss of expression of the DNA mismatch repair protein MLH1. The evolution of this subset of CRC is unclear as a preceding adenoma phase is very rarely seen. Recently, studies by us and others have provided molecular evidences to suggest that there may be a strong link between the serrated neoplasia pathway and the development of sporadic CRC with MSI. These include the identification of a high incidence of BRAF mutation in HPs and SAs, the occurrence of MSI in some SPs and the selective association of BRAF mutation with sporadic late-onset CRC with MSI. Our recent pilot study has shown that MLH1 inactivation can be detected in SPs at its very early phase and these can just involve several crypts within the lesion. With these data, we propose to perform a large scale phenotypic and molecular characterization of serrated polyps at their early phase of evolution to define the link between SPs and MSI CRC, and the temporal sequence of genetic changes in this pathway. Aims:1. To look for evidence of MLH1 inactivation in various stages of evolution of serrated polyps, and to document its incidence and phenotypic characteristics.2. To document the occurrence of microsatellite instability and frameshift mutation in growth regulatory genes containing microsatellite encoding region as consequences of MLH1 inactivation in various stages of serrated polyps.3. To analyse the inter-relationship between MLH1 inactivation, BRAF/KRAS mutations and presence of the CpG Island Methylator Phenotype in SPs and their temporal sequence of occurrence.4. To look for alteration in major molecular genetic pathways in SPs with MLH1 inactivation.5. To look for evidence of a field effect of MLH1 inactivation in the colon in patients with sporadic late-onset MSI colorectal cancer with MLH1 promoter methylation.6. To look for clinical, morphological or molecular markers that can distinguish SPs with high risk of progression to MSI CRC.

List of Research Outputs

Li V.S.W., Wong C.W., Chan T.L., Chan A.S.W., Zhao W., Chu K.M., Chen X., Yuen S.T., Leung S.Y. and So S., Mutations of PIK3CA in gastric adenocarcinoma, BioMed Central Cancer. 2005, 5: 29.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Wong C.W., Fan Y.S., Chan T.L., Chan A.S.W., Ho L.C., Ma T.K.F., The Cancer Genome Project , Yuen S.T. and Leung S.Y., BRAF and NRAS mutations are uncommon in melanomas arising in diverse internal organs, Journal of Clinical Pathology. 2005, 58: 640-644.

Researcher : Chan YK

Project Title:	Study of metastasis suppressing genes in metastatic ovarian cancer
Investigator(s):	Chan YK, Ngan HYS, Cheung ANY, Khoo US
Department:	Obstetrics and Gynaecology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To investigate the role of the metastasis suppressing genes in the metastasis ovarian cancer. The epigenetic factor of promoter hypermethylation in relation to the expression of the metastasis suppressing gene will aso be investigated.

List of Research Outputs

Cheung A.N.Y., Chiu P.M., Chan Q.K.Y., Chan Y.K. and Ngan H.Y.S., Stem cell related genes and malignant progression in gestational trophoblastic diseases, In: 105943, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Researcher : Cheung ANY

Project Title:	Methylation and expression analysis of p33/ING1 tumor-suppressor gene in ovarian endometrial and ovarian cancer
Investigator(s):	Cheung ANY, Ngan HYS
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To investigate whether the status of expression and methylation of p33/ING1 contributes significantly in hte pathogenesis and progression of endometrial and ovarian cancer.

Project Title:	DNA methylation and histone acetylation profile in gestational trophoblastic
Investigator(s):	Cheung ANY, Ngan HYS, Khoo US, Chan YK
Department:	Pathology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To analyze the DNA methylation and histone acetylation status of p53, mdm2, p21^WAF, Mc1-1,bc1-2, p16, E-cadherin, caspases 8 and 10 in hydatidiform mole in an attempt to evaluate the role of such epigenetic changes in the pathogenesis of trophoblastic disease; to recognize genetic markers that may be able to predict the clinical progression of hydatidiform mole; to explore potential use of DNA methylation and histone acetylation inhibitors in future treatment.

Project Title:	Pi-class Glutathione-S-Transferase in endometrial carcinoma
Investigator(s):	Cheung ANY, Ngan HYS, Khoo US
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To investigate whether the genetic alteration, status of expression and methylation of GSTP1 contributes significantly to the pathogenesis and progression of endometrial cancer.

Project Title:	Stem cell related genes in gynaecological cancers
Investigator(s):	Cheung ANY, Ngan HYS
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To characterize the expression levels and epigenetic alterations of important stem-cell related genes in ovarian and endometrial cancers in an attempt to evaluate the roles of such genes in the their carcinogenesis; and to define useful genetic markers as predictors of clinical progression or targets for therapy.

Project Title:	Stem cell related genes in gestational trophoblastic diseases
Investigator(s):	Cheung ANY, Ngan HYS, Chan YK, Yeung WSB
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To characterize the expression levels and epigenetic alterations of important stem-cell related genes in gestational trophoblastic disease (GTD) in an attempt to evaluate the roles of such genes in the pathogenesis of GTD; and to define useful genetic markers as predictors of clinical progression or targets for therapy.

Project Title:	Stem cell related genes in gestational trophoblastic diseases
Investigator(s):	Cheung ANY
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Ajonuma L.C., Ng E.H.Y., Chow P.H., Hung C.Y., Tsang L.L., Cheung A.N.Y., Brito-Jones C., Lok I.H., Haines C.J. and Chan H.C., Increased cystic fibrosis transmembrane conductance regulator (CFTR) expression in the human hydrosalpinx, Human Reproduction. 2005, 20(5): 1228-1234.

Chan Q.K.Y., Khoo U.S., Chan Y.K., Ngan H.Y.S., Li S.S., Chiu P.M., Man M.L.S., Ip P.P.C., Xue W. and Cheung A.N.Y., Promoter methylation and differential expression of p-Class glutathione S-transferase in endometrial carcinoma, The Journal of Molecular Diagnostics. 2005, 7(1): 8-16.

Chan Q.K.Y., Khoo U.S., Ngan H.Y.S., Yang C., Xue W., Chan Y.K., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Single nucleotide polymorphism of Pi-Class glutathione S-transferase and susceptibility to endometrial carcinoma, Clinical Cancer Research. 2005, 11(8): 2981-2985.

Chan Y.K., Liu S., Leung C.Y., Cheung A.N.Y., Khoo U.S., Leung T.W. and Ngan H.Y.S., Promoter methylation of Delta-TA isoform of p73 regulating the corresponding transcript expression level in gynecological cancers, 70th Cold Spring Harbor Laboratory Symposium: Molecular Approaches to Controlling Cancer, New York, USA, June 1-6, 2005.

Cheung A.N.Y., Chiu P.M., Tsun O.K.L., Khoo U.S., Leung B.S.Y. and Ngan H.Y.S., Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology, Journal of Clinical Pathology. 2004, 57: 721-727.

Cheung A.N.Y., Differential gene expression and methylation status as predicting indicators for gestational trophoblastic neoplasia, The Croucher Advanced Study Institute on “Molecular Genetics Cell Signaling in Cancers” Hong Kong, 17-21 January 2005.

Cheung A.N.Y., Chiu P.M., Chan Q.K.Y., Chan Y.K. and Ngan H.Y.S., Stem cell related genes and malignant progression in gestational trophoblastic diseases, In: 105943, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Feng H., Choy M.Y., Deng W., Wong H.L., Lau W.M., Cheung A.N.Y., Ngan H.Y.S. and Tsao G.S.W., Establishment and characterization of a human first-trimester extravillous trophoblast cell line (TEV-1), Journal of The Society for Gynecologic Investigation. 2005, 12(4): e21-32.

Fong P.Y., Xue W., Ngan H.Y.S., Chan Y.K., Khoo U.S., Tsao G.S.W., Chiu P.M., Man M.L.S. and Cheung A.N.Y., Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome, Cancer. 2004, 103(2): 268-276.

Khoo U.S., Chan Y.K., Peiris J.S.M., Yip S.P., Liu W., Ngan H.Y.S., Tam P.K.H., Chan L.C. and Cheung A.N.Y., Association of ICAM3 genetic variant with Severe Acute Respiratory Syndrome (SARS)., HUGO's 10th Human Genome Meeting, 18-21 April 2005, Kyoto, Japan. 2005.

Li R.H.W., Yu M.M.Y., Cheung A.N.Y. and Wong Y.F., Expression of leptin and leptin receptors in gestational trophoblastic diseases, Gynecologic Oncology. 2004, 95(2): 299-306.

Li S.S., Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Tam I.Y.S., Chiu P.M., Ip P.P.C., Tam K.F. and Cheung A.N.Y., Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: A tissue microarray and clinicopathological analysis, Histopathology. 2005, 46(3): 307-313.

Liu V.W.S., Chiu P.M., Yao K.M., Chan D.W., Hui C.C., Cheung A.N.Y. and Ngan H.Y.S., Study of hedgehog signaling in cervical cancer, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Liu W., Chan Y.K., Chan S.Y., Yip S.P., Cheung A.N.Y., Chua D.T.T., Ngan H.Y.S. and Khoo U.S., BRCA1 gene promoter polymorphisms associated with breast cancer risk, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Shen D.H., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Epigenetic and genetic alterations of p33ING1b in ovarian cancer., In: Shen DH*, Chan YK*, Khoo US, Ngan HY, Xue WC, Chiu PM, Ip PP, Cheung AN. , Carcinogenesis . 2005, 26: 855-63.

Wang Y., Liu V.W.S., Tsang P.C.K., Xue W.C., Cheung A.N.Y. and Ngan H.Y.S., Elevated copy number and extensive microsatellite instability in mitochondrial genome of human endometrial adenocarcinoma, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Xue W., Chan Y.K., Feng H., Chiu P.M., Ngan H.Y.S., Tsao G.S.W. and Cheung A.N.Y., Promoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinoma, The Journal of Molecular Diagnostics. 2004, 6(4): 326-334.

Researcher : Cheung N

List of Research Outputs

Cheung N., So C.W., Yam J.W.P., So C.K.C., Poon R.Y.C., Jin D. and Chan L.C., Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukemia, Biochemical Journal. 2004, 383(Pt 1): 27-35.

Researcher : Chiang AKS

Project Title:	Experimental immunotherapy of tumours
Investigator(s):	Chiang AKS
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Research Initiation Programme
Start Date:	04/2001

Abstract:

To develop strategies in enhancing the host immune response against tumours.

Project Title:	Immunstimulatory effects of different microbial components on maturation and differentiation of dendritic cell subsets
Investigator(s):	Chiang AKS, Chan GCF, Lau ASY
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Low Budget High Impact Programme
Start Date:	11/2001
Completion Date:	04/2005

Abstract:

To characterize the effects of different microbial components including nucleic acids on the maturation and survival of DC subsets, pDC1 and pDC2. The T cell polarization effect and the mechanisms of signal transduction would be further studied.

Project Title:	Genetic studies of tubercubosis
Investigator(s):	Chiang AKS, Tang N., Yew W.W., Tam C.M.
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Other Funding Scheme
Start Date:	03/2002

Abstract:

To host susceptibility genes in tubercubosis; to study pharmacogenomics in anti-tubercubosis drug metabolism.

Project Title:	Prospective study of virologic and immunologic parameters of primary Epstein-Barr virus infection in Chinese children
Investigator(s):	Chiang AKS, Chan KH
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	10/2003

Abstract:

An unresolved issue in EBV immunology concerns the relationship between clinical IM-like symtoms, virus DNA load and CD8+ T cell lymphocytosis; Acute IM in adolescents is associated with large expansions of EBV lytic epitope reactivities which are followed by a later expansion of latent epitope responses. The magnitude of the EBV lytic and latent epitope reactivities would be studied and compared in both IM and non-IM patients; likewise, is there a difference in the rate of emergence of latent epitope responses bewteen the IM and non -IM patients? The kinetics and evolution of the EBV epitope-specific cytotoxic T-lymphocyte (CTL) responses from primary to persistent phases of EBV infection would be compared between the two groups of children.

Project Title:	Prospective study of virologic and immunologic parameters of primary Epstein-Barr virus infection in Chinese children
Investigator(s):	Chiang AKS, Chan KH
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	10/2003

Abstract:

Project Title:	Host susceptibility genes in tuberculosis
Investigator(s):	Chiang AKS, Lau YL
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To investigate the genetic susceptibility of the Chinese population to tuberculosis.

Project Title:	Association, linkage and functional studies of IL-12P40 polymorphisms in human tuberculosis susceptibility
Investigator(s):	Chiang AKS
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

Project Title:	Prospective study of Epstein-Barr virus (EBV) strains in primary EBV infection
Investigator(s):	Chiang AKS
Department:	Paediatrics & Adolescent Medicine
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To study the occurrence of Epstein-Barr virus (EBV) diversity and coinfection in a prospective study of primary Epstein-Barr virus infection in Chinese children.

Researcher : Ching YP

Project Title:	The functional characterisation of Pak5 and caspase 4 interaction- a role in apoptosis
Investigator(s):	Ching YP
Department:	Pathology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2004

Abstract:

To confirm the interaction of Pak5 and caspase 4 using three independent assays, including co-immunoprecipitation, co-immunostaining and GST affinity pull down assays; to map the minimal binding region of these two proteins and develop functional assays to assess the functional outcome of this interaction; to determine if Pak5 plays a role in apoptosis by regulating the caspase 4.

Project Title:	Roles and regulation of group II p21-activated protein kinases:-implications in cancer metastasis
Investigator(s):	Ching YP, Jin D, Ng IOL
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To study: (1) Characterisation of the interaction between Pak5 and NM23 i) co-immunoprecipitation of Pak5 adn NM23 ii) defining the binding domain between Pak 5 and NM23 iii) xploring the interaction between Pak4 adn NM23. (2) Impact of Pak5-NM23 interaction in the biochemical properties of Pak5 and NM23 i) nucleotide diphosphate kinase activity ii) GTPase activating activity iii) in vitro kinase activity iv) subcellular localisation. 3) Roles of PakII in cancer metastasis using HCC as a model i) expression profile of Pak4 in HCC ii) clinicopathological analysis iii) cell invasion assay.

Project Title:	Roles and regulation of group II p21-activated protein kinases:-implications in cancer metastasis
Investigator(s):	Ching YP
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Ching Y.P., Wong C.M., Jin D. and Ng I.O.L., Identification of a novel RHO GTPASE activating protein called DLC2, involved in hepatocellular carcinoma, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.

Ching Y.P., Identification of an autoinhibitory domain in P21-activated protain kinase 5, Joint Research Retreat for Young Scientists, HKU - Cambridge - HKUST, 10-12 March 2005 . 2005.

Ching Y.P., Chun C.S., Chin K.T., Zhang Z.Q., Jeang K.T. and Jin D., Specific TATAA and bZIP requirements suggest that HTLV-I Tax has transcriptional activity subsequent to the assembly of an initiation complex, Retrovirology. 2004, 1(18): 1-12.

Kok K.H., Choy E.Y.W., Ching Y.P. and Jin D., Cell-to-cell spreading of RNA interference in mammalian cells, The ASCB 44th Annual Meeting, Washington, DC, USA, 4-8 December 2004.

Leung T.H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Identification of multiple isoforms of DLC2, a novel tumor suppressor gene, and their functional characterization in hepatocellular carcinoma, 4th International Meeting of Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, December 2004 (Young Investigator Award). 2004.

Li H.Y., Fung K.L., Ching Y.P., Ng I.O.L., Chung S.S.M., Sze K.H., Ko B.C.B. and Sun H., Structural study of the SAM domain of the deleted in liver cancer 2 (DLC2), Chemistry Symposium of Hong Kong, March 2005.

Ng D.C.H., Ching Y.P., Ng I.O.L. and Jin D., Functional characterization of a Novel RhoGAP protein deleted in liver cancer 2 (DLC2), 9th research postgraduate symposium, HKU, December 4, 2004.

Ng D.C.H., Ching Y.P., Chan S.F. and Jin D., Human T-cell leukemia virus type I oncoprotein tax targets the centrosome, 10th SCBA International Symposium, Beijing, China18-23 July 2004.

Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Association of MAD2 Expression with Mitotic Checkpoint Competence in Hepatoma Cells, J. Biomed. Sci. . 2004, 11: 920-7.

Wong C.M., Ching Y.P. and Ng I.O.L., Genome-wide methylation screening in search for novel tumor suppressor genes in liver cancer, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Researcher : Chiu PM

List of Research Outputs

Chan Q.K.Y., Khoo U.S., Chan Y.K., Ngan H.Y.S., Li S.S., Chiu P.M., Man M.L.S., Ip P.P.C., Xue W. and Cheung A.N.Y., Promoter methylation and differential expression of p-Class glutathione S-transferase in endometrial carcinoma, The Journal of Molecular Diagnostics. 2005, 7(1): 8-16.

Chan Q.K.Y., Khoo U.S., Ngan H.Y.S., Yang C., Xue W., Chan Y.K., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Single nucleotide polymorphism of Pi-Class glutathione S-transferase and susceptibility to endometrial carcinoma, Clinical Cancer Research. 2005, 11(8): 2981-2985.

Cheung A.N.Y., Chiu P.M., Tsun O.K.L., Khoo U.S., Leung B.S.Y. and Ngan H.Y.S., Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology, Journal of Clinical Pathology. 2004, 57: 721-727.

Cheung A.N.Y., Chiu P.M., Chan Q.K.Y., Chan Y.K. and Ngan H.Y.S., Stem cell related genes and malignant progression in gestational trophoblastic diseases, In: 105943, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Fong P.Y., Xue W., Ngan H.Y.S., Chan Y.K., Khoo U.S., Tsao G.S.W., Chiu P.M., Man M.L.S. and Cheung A.N.Y., Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome, Cancer. 2004, 103(2): 268-276.

Li S.S., Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Tam I.Y.S., Chiu P.M., Ip P.P.C., Tam K.F. and Cheung A.N.Y., Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: A tissue microarray and clinicopathological analysis, Histopathology. 2005, 46(3): 307-313.

Liu V.W.S., Chiu P.M., Yao K.M., Chan D.W., Hui C.C., Cheung A.N.Y. and Ngan H.Y.S., Study of hedgehog signaling in cervical cancer, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Shen D.H., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Epigenetic and genetic alterations of p33ING1b in ovarian cancer., In: Shen DH*, Chan YK*, Khoo US, Ngan HY, Xue WC, Chiu PM, Ip PP, Cheung AN. , Carcinogenesis . 2005, 26: 855-63.

Xue W., Chan Y.K., Feng H., Chiu P.M., Ngan H.Y.S., Tsao G.S.W. and Cheung A.N.Y., Promoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinoma, The Journal of Molecular Diagnostics. 2004, 6(4): 326-334.

Researcher : Chiu PM

List of Research Outputs

Researcher : Chow EYD

List of Research Outputs

Wan T.S.K., Ma E.S.K., Chow E.Y.D., Li Y.H., Lin S.Y. and Chan L.C., Pathogenesis of jumping translocations: a molecular cytogenetics study, Leukemia Research. 2004, 28(10): 1075-1079.

Researcher : Chung LP

List of Research Outputs

Au W.Y., Srivastava G., Wong K.Y., Chung L.P., Ma E.S.K., Wan T.S.K. and Kwong Y.L., Transformation of diffuse large B-cell lymphoma into pre-B acute lymphoblastic leukemia: clinicopathologic features and clonal relationship, Human Pathology. 2004, 35(7): 900-903.

Lam C.L., Wong M.P., Girard L., Chung L.P., Chau W.S., Chiu S.W., Lam W.K. and Minna J.D., Gene expression profiling in lung adenocarcinomas reveals molecular signatures of potential biological significance , Journal of The Japanese Respiratory Society. 2005, 43 (Suppl): 115 (EO6-3).

Lam D.C.L., Wong M.P., Girard L., Shigematsu H., Chung L.P., Gazdar A.F., Chiu S.W., Suen W.S., Lam W.K. and Minna J.D., Expression profiling in lung adenocarcinoma with or without Epidermal Growth Factor Receptor (EGFR) gene mutation at exons 18-21 reveals expression signatures related to the EGFR pathway, 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, P.O.CB 39: 888/B#9.

Lam D.C.L., Wong M.P., Girard L., Chung L.P., Chau W.S., Chiu S.W., Lam W.K. and Minna J.D., Gene expression profiling in lung adenocarcinomas reveals molecular signatures of potential biological significance, Respirology. 2004, 9 (Suppl): A121 (236).

Tam I.Y.S., Wong M.P., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., EGFR mutations are prevalent and independent from K-RAS mutations in lung adenocarcinomas from non-smokers, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Tam I.Y.S., Wong M.P., Suen W.S., Wang E., Lam W.K., Chiu S.W., Gazdar A., Minna J.D. and Chung L.P., EGFR mutations are prevalent and independent from k-RAS mutations in lung adenocarcinomas from non-smokers, 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, M.S.CL00.01: 1666.

Wong M.P., Lam D.C.L., Yap D.Y.L., Girard L., Tam I.Y.S., Chung L.P., Chiu S.W., Lam W.K., Danchin A. and Minna J.D., Identification of discriminating gene expression of EBV-associated primary lymphoepitheioma-like carcinoma of lung by oligonucleotide microarray analysis , 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, P.O.CB19: 75/B#3.

Yap Y.L., Lam C.L., Girard L., Zhang X., Hernandez D., Gras R., Wang E., Chiu S. .W., Chung L.P., Lam W.K., Smith D.K., Minna J. .D., Danchin A. and Wong M.P., Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays, Nucleic Acids Research. Oxford University Press 2005, 2004, 33 No. 8: 2764.

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., High-resolution analysis of DNA copy number alterations and expression profiling by microarray technology in primary lung cancer cell lines, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Researcher : Fong PY

List of Research Outputs

Fong P.Y., Xue W., Ngan H.Y.S., Chan Y.K., Khoo U.S., Tsao G.S.W., Chiu P.M., Man M.L.S. and Cheung A.N.Y., Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome, Cancer. 2004, 103(2): 268-276.

Researcher : Hawkins BR

List of Research Outputs

Au W.Y., Lie A.K.W., Kung A.W.C., Liang R.H.S., Hawkins B.R. and Kwong Y.L., Autoimmune thyroid dysfunction after hematopoietic stem cell transplantation, Bone Marrow Transplantation. 2005, 35(4): 383-8.

Kwok J.S.Y., Leung A.Y.H., Lie A.K.W., Lee T.L., Lau Y.L., Chu P., Jones B.M., Hawkins B.R. and Liang R.H.S., Antirecipient helper and cytotoxic T-cell frequencies in bone marrow transplantation, Bone Marrow Transplantation. 2004, 34(3): 207-213.

Researcher : Higgins DA

List of Research Outputs

Liang Y., Zhang J.C.L., Higgins D.A. and Chan S.Y., Functional characteristics of SUN-2, 9th Research Postgraduate Symposium, HKU, 4 December 2004. 104.

Researcher : Ho WL

List of Research Outputs

Lai A.T.Y., Lam C.M., Ng K.K.C., Yeung C., Ho W.L., Poon L.T. and Ng I.O.L., Hepatic actinomycosis presenting as a liver tumour: Case report and literature review, Asian Journal of Surgery. 2004, 27(4): 345-347.

Researcher : Huang F

Project Title:	The roles of regulatory dendritic cells in peripheral tolerance and autoimmunity
Investigator(s):	Huang F, MacPherson G.G.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2001

Abstract:

To create in vivo models which mimic and auto- or host-reactive environment; to visualise the fate of potential self-reactive T cells in normal and in autoimmune-prone mice; to study the role of DCreg in the generation of Treg cells; to study DC functions in autoimmune models; to generate DCreg and Treg for the treatment of autoimmune diseases.

Project Title:	Functional conditioning of dendritic cells for DC-based tumor vaccine
Investigator(s):	Huang F, Tian L, Ng IOL
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2003

Abstract:

Dendritic cell (DC)-based tumor vaccine is a newly developed therapeutic approach for cancer treatment. It aims to promote specific immunity to cancer cells within tumor bearing individuals. Recent studies reveal that DC are not a homogenous population, and their ability to provide activation signals can vary significantly between different DC subtypes, lineages or maturity. The types and functional conditions, hence the immunogenic 'quality', of the DC employed are understandably essential. This project is to examine critically the roles of co-stimulatory molecules in DC-based tumor vaccines. The main objective is to develop immunologically active and programmable DC-tumor vaccines with high efficacy useful in cancer therapies.

Project Title:	Mechanisms for the induction and regulation of autoimmune responses by dendritic cells and T regulatory cells in systemic lupus erythematosus
Investigator(s):	Huang F, Wu AYY, Tian L
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	12/2003

Abstract:

To study the mechanisms for induction and regulation of autoimmune responses; to study effects of cell death on the induction of anti-nuclear antibody production, in normal and in autoimmune-prone mice; to test the hypothesis that uptake of necrotic or apoptotic dying cells by dendritic cells may differentially regulate autoimmune responses in vivo; to determine the roles, and mechanisms for generation, of the naturally occurring T regulatory cells in autoimmune and non-autoimmune mice.

Project Title:	Mechanisms for the induction and regulation of autoimmune responses by dendritic cells and T regulatory cells in systemic lupus erythematosus
Investigator(s):	Huang F, Wu AYY, Tian L
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2003

Abstract:

Project Title:	Molecular characterization of dendritic cells functionally modulated by dying cells - mechanism for the induction and regulation of autoimmune responses by dendritic cells (II)
Investigator(s):	Huang F, Leung SY, Wu AYY, Tian L
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To study the molecular mechanisms for the induction of autoimmune responses by DCs; to study the immune response profiles of DCs after uptake of dying cells; to determine how DC functional properties can be differentially modulated following uptake of apoptotic and necrotic dying cells; to determine the therapeutic potential of functionally conditioned DC for a possible long-term rectification of the autoimmune disorder.

Project Title:	Molecular characterization of dendritic cells functionally modulated by dying cells - mechanism for the induction and regulation of autoimmune responses by dendritic cells (II)
Investigator(s):	Huang F
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Chiang A.K.S., Wong O.H. and Huang F., Differential responses of cord and adult blood-derived dendritic cells to dying cells, The 3^rd Congress of the Federation of Immunology Societies of Asia-Oceania, Hangzhou, PR China, 18-22 April 2005. C95.

Huang F., Dendritic Cells: Friends, Foes and the ‘Trojan Horse’, Presented at the Hong Kong Society of Haematology, Hong Kong, 28 January 2005. 2005.

Huang F., Induction of systemic autoimmune disease by dendritic cells that have captured dying cells, Proceedings of the 11th Asia Pacific League of Associations for Rheumatology Congress (APLAR 2004), International Convention Center, (ICC), Jeju, Korea, September 11-16, 2004. 2004.

Huang F., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Presented at Sir William Dunn School of Pathology, University of Oxford, UK, 3 August 2004. 2004.

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Ma L., Ng M.H. and Huang F., Interactions of dendritic cells and dying cells in Systemic Lupus Erythematosus, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Ma L., Chan K.W., Trendell-Smith N.J., Lo C.K., To K.W. and Huang F., Necrotic cells induce systemic autoimmune disease in vivo by activation of dendritic cells, 62th Annual Meeting, American Academy of Allergy, Asthma & Immunology (AAAAI), San Antonio, FL, USA, 3-7 March 2005.

MacPherson G., Milling S., Yrlid U., Cousins L., Turnbull E. and Huang F., Uptake of antigens from the intestine by dendritic cells, Annals of the New York Academy of Sciences. 2004, 1029: 75-82.

Sun Q., Huang F. and Chan L.C., Dendritic cells differentiation from cells of leukemia origin, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

To K.W., Chiang A.K.S. and Huang F., Tumor derived immuno-suppressive molecules on dendritic cells (DC) functions and the implications in DC-based vaccine, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Wong O.H., Huang F. and Chiang A.K.S., Role of dendritic cell in the immune regulation of primary epstein-barr virus infection, Proceedings of the 12th International Congress of Immunology, 18-23 July 2004, Montreal. 2004.

Yang C. and Huang F., Regulation of autoimmune responses by dendritic cells and T regulatory cells in Systemic Lupus Erythematosus, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Researcher : Ip PPC

List of Research Outputs

Chan Q.K.Y., Khoo U.S., Chan Y.K., Ngan H.Y.S., Li S.S., Chiu P.M., Man M.L.S., Ip P.P.C., Xue W. and Cheung A.N.Y., Promoter methylation and differential expression of p-Class glutathione S-transferase in endometrial carcinoma, The Journal of Molecular Diagnostics. 2005, 7(1): 8-16.

Chan Q.K.Y., Khoo U.S., Ngan H.Y.S., Yang C., Xue W., Chan Y.K., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Single nucleotide polymorphism of Pi-Class glutathione S-transferase and susceptibility to endometrial carcinoma, Clinical Cancer Research. 2005, 11(8): 2981-2985.

Li S.S., Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Tam I.Y.S., Chiu P.M., Ip P.P.C., Tam K.F. and Cheung A.N.Y., Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: A tissue microarray and clinicopathological analysis, Histopathology. 2005, 46(3): 307-313.

Shen D.H., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Epigenetic and genetic alterations of p33ING1b in ovarian cancer., In: Shen DH*, Chan YK*, Khoo US, Ngan HY, Xue WC, Chiu PM, Ip PP, Cheung AN. , Carcinogenesis . 2005, 26: 855-63.

Researcher : Jones BM

List of Research Outputs

Chiang A.K.S., Ma E.S.K., Jones B.M., Li Y.H., Chan G.C.F., Ha S.Y. and Lau Y.L., Long Term Immune Reconstitution after Bone Marrow Transplantation for Severe Combined Immunodeficiency and Wiskott Aldrich Syndrome, 5th Guangdong - Hong Kong Paediatric Exchange Meeting, HK, 24 July 2004. 第五屆粵港兒科學術交流會，香港， 24 July 2004, 47.

Jones B.M., Chiu S.S.S., Wong W.H.S., Lim W.W.L. and Lau Y.L., Cytokine profile in human immunodeficiency Viurs-infected children treated with highly active Antiretroviral therapy, Medscape General Medicine. 2005, 7(1): @2005.

Kim D.L., Wong R.W.S., Jones B.M., Lau W.C.S. and Kim T.Y., Antiperinuclear factor in Chinese: does antiperinuclear factor have the same implication in Chinese and Western populations?, 11th APLAR Bi-annual Congress, Jeju, Korea 11-16 September 2004.

Kwok J.S.Y., Leung A.Y.H., Lie A.K.W., Lee T.L., Lau Y.L., Chu P., Jones B.M., Hawkins B.R. and Liang R.H.S., Antirecipient helper and cytotoxic T-cell frequencies in bone marrow transplantation, Bone Marrow Transplantation. 2004, 34(3): 207-213.

Kwok J.S.Y. and Jones B.M., Unnecessary repeat requesting of tests: an audit in a government hospital immunology laboratory, Journal of Clinical Pathology. 2005, 58: 457-462.

Researcher : Khoo US

Project Title:	Detection of breast cancer cells in fine needle aspirates by methylation-specific polymerase-chain-reaction
Investigator(s):	Khoo US
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To find out if methylation analysis could be a useful adjunct to identification of breast cancer cells in fine needle aspiration cytology (FNAC) samples taken from lesions of the breast.

Project Title:	Association of the pro-inflammatory and anti-inflammatory cytokine gene polymorphism to breast cancer susceptibility
Investigator(s):	Khoo US, Cheung ANY, Chan YK, Yip SP
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To determine whether the genetic variants of the pro-inflammatory and anti-inflammatory cytokines may (a) contribute towards breast cancer susceptibility or (b) influence prognosis; to investigate whether the joint effects of several of these alleles and/or in combination of specific environmental factors may contribute towards a stronger association.

Project Title:	Promoter polymorphisms of BRCA1: genetic association and functional study
Investigator(s):	Khoo US, Chan YK, Cheung ANY
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To perform a case-control study to investigate the promoter polymorphisms BRCA1 gene for risk association to breast cancer; to test by Luciferase report assay whether these promoter SNPs result in functional alteration of the BRCA1 gene.

List of Research Outputs

Chan Q.K.Y., Khoo U.S., Chan Y.K., Ngan H.Y.S., Li S.S., Chiu P.M., Man M.L.S., Ip P.P.C., Xue W. and Cheung A.N.Y., Promoter methylation and differential expression of p-Class glutathione S-transferase in endometrial carcinoma, The Journal of Molecular Diagnostics. 2005, 7(1): 8-16.

Chan Q.K.Y., Khoo U.S., Ngan H.Y.S., Yang C., Xue W., Chan Y.K., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Single nucleotide polymorphism of Pi-Class glutathione S-transferase and susceptibility to endometrial carcinoma, Clinical Cancer Research. 2005, 11(8): 2981-2985.

Chan Y.K., Liu S., Leung C.Y., Cheung A.N.Y., Khoo U.S., Leung T.W. and Ngan H.Y.S., Promoter methylation of Delta-TA isoform of p73 regulating the corresponding transcript expression level in gynecological cancers, 70th Cold Spring Harbor Laboratory Symposium: Molecular Approaches to Controlling Cancer, New York, USA, June 1-6, 2005.

Cheung A.N.Y., Chiu P.M., Tsun O.K.L., Khoo U.S., Leung B.S.Y. and Ngan H.Y.S., Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology, Journal of Clinical Pathology. 2004, 57: 721-727.

Fong P.Y., Xue W., Ngan H.Y.S., Chan Y.K., Khoo U.S., Tsao G.S.W., Chiu P.M., Man M.L.S. and Cheung A.N.Y., Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome, Cancer. 2004, 103(2): 268-276.

Khoo U.S., Chan Y.K., Peiris J.S.M., Yip S.P., Liu W., Ngan H.Y.S., Tam P.K.H., Chan L.C. and Cheung A.N.Y., Association of ICAM3 genetic variant with Severe Acute Respiratory Syndrome (SARS)., HUGO's 10th Human Genome Meeting, 18-21 April 2005, Kyoto, Japan. 2005.

Lam T.P., Khoo U.S., Chan Y.S., Cheng Y.H. and Lam K.F., The first batch of graduates of a new medical curriculum in Asia: how their teachers see them, Medical Education. 2004, 38(9): 980-986.

Li S.S., Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Tam I.Y.S., Chiu P.M., Ip P.P.C., Tam K.F. and Cheung A.N.Y., Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: A tissue microarray and clinicopathological analysis, Histopathology. 2005, 46(3): 307-313.

Liu W., Chan Y.K., Chan S.Y., Yip S.P., Cheung A.N.Y., Chua D.T.T., Ngan H.Y.S. and Khoo U.S., BRCA1 gene promoter polymorphisms associated with breast cancer risk, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Shen D.H., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Epigenetic and genetic alterations of p33ING1b in ovarian cancer., In: Shen DH*, Chan YK*, Khoo US, Ngan HY, Xue WC, Chiu PM, Ip PP, Cheung AN. , Carcinogenesis . 2005, 26: 855-63.

Researcher : Ko KH

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Lu L., Zhang M., Cheung C.T., Wong C., Ko K.H., Tsang S.L., Chan L.C. and Sham M.H., Hoxb3 deficiency impairs B lymphopoiesis, 12th International Conference for Immunology, Montreal, Canada, July 18-23, 2004. 2004.

Lu L., Lam Q.L.K., Zhang M., Lo K.C., Ko K.H., Osmond D.G., Wu G.E. and Rottapel R., Novel function of c-Abl in regulating precursor B cell development, The 3rd Congress of the Federation of Immunology Societies of Asia-Oceania, Hangzhou, China, April 18-22, 2005.

Zhang M., Ko K.H., Sham M.H. and Lu L., Novel function of HOXB3 gene in regulation B lymphopoiesis , 9th research postgraduate symposium, HKU, December 4, 2004.

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Researcher : Kong CT

List of Research Outputs

Chan L.C., Kong C.T. and Sham M.H., The leukaemia fusion gene, Mll-Een affects haemopoietic development in mouse embryonic stem cells with enhanced proliferation and self renewal of myeloid progenitors, Days of Molecular Medicine 2005 – Stem Cell Biology and Human Disease, 17-19 March 2005, Salk Institute, La Jolla, California, USA. 2005.

Researcher : Kwok JSY

List of Research Outputs

Au W.Y., Kwok J.S.Y., Chu K.M. and Ma E.S.K., Life-threatening cryoglobulinemia in HCV negative Southern Chinese and a novel association with structural aortic abnormalities, Annals of Hematology. 2005, 84: 95-98.

Kwok J.S.Y., Leung A.Y.H., Lie A.K.W., Lee T.L., Lau Y.L., Chu P., Jones B.M., Hawkins B.R. and Liang R.H.S., Antirecipient helper and cytotoxic T-cell frequencies in bone marrow transplantation, Bone Marrow Transplantation. 2004, 34(3): 207-213.

Kwok J.S.Y. and Jones B.M., Unnecessary repeat requesting of tests: an audit in a government hospital immunology laboratory, Journal of Clinical Pathology. 2005, 58: 457-462.

Lee T.L., Kwok J.S.Y. and Lau Y.L., A Child with Behcet's Disease Associated with Natural Killer Cell Deficiency, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 48.

Researcher : Kwong THG

List of Research Outputs

Kwong T.H.G., An Autopsy-based Epidemiological Study of Road Traffic Fatalities in Hong Kong: Crash Type, Injury Severity and Prospects for Intervention. 2004.

Researcher : Lam AC

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Researcher : Lam CCK

List of Research Outputs

Au W.Y., Ma S.Y., Chim J.C.S., Choy C., Loong F., Lie A.K.W., Lam C.C.K., Leung A.Y.H., Tse E.W.C., Yau C.C., Liang R.H.S. and Kwong Y.L., Clinicopathologic features and treatment outcome of mature T-cell and natural killer-cell lymphomas diagnosed according to the World Health Organization classification scheme: a single center experience of 10 years, Annals of Oncology. 2005, 16(2): 206-214.

Chim J.C.S., Wong S.S.Y., Lam C.C.K. and Chan K.W., Concurrent hyperreactive malarial splenomegaly and quartan malarial nephropathy – Plasmodium malariae revisited, Haematologica. 2004, 89(7): ECR21.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Researcher : Lam CL

List of Research Outputs

Girard L., Lam C.L., Shigematsu H., Wong M.P., Peyton M., Sheridan S., Beer D.G., Gazdar A.F. and Minna J.D., Gene profiling of lung cancers with EGFR mutations, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Yap Y.L., Lam C.L., Girard L., Zhang X., Hernandez D., Gras R., Wang E., Chiu S. .W., Chung L.P., Lam W.K., Smith D.K., Minna J. .D., Danchin A. and Wong M.P., Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays, Nucleic Acids Research. Oxford University Press 2005, 2004, 33 No. 8: 2764.

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., High-resolution analysis of DNA copy number alterations and expression profiling by microarray technology in primary lung cancer cell lines, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Researcher : Lam CL

List of Research Outputs

Researcher : Lam LKQ

List of Research Outputs

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Researcher : Lam QLK

List of Research Outputs

Lu L., Lam Q.L.K., Zhang M., Lo K.C., Ko K.H., Osmond D.G., Wu G.E. and Rottapel R., Novel function of c-Abl in regulating precursor B cell development, The 3rd Congress of the Federation of Immunology Societies of Asia-Oceania, Hangzhou, China, April 18-22, 2005.

Researcher : Lee MF

List of Research Outputs

Chan D.W., Lee M.F., Chan P.C.Y. and Ng I.O.L., T-cadherin is silenced by frequent genetic and epigenetic alterations in hepatocellular carcinoma, The 11th HK International Cancer Congress, 10-12 November 2004, Hong Kong. 2004.

Chin K.T., Zhou H., Wong C.M., Lee M.F., Chan C.P., Qiang B.Q., Yuan J.G., Ng I.O.L. and Jin D., The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma, Nucleic Acids Research. 2005, 33(6): 1859-1873.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Frequent down-regulation of HDPR1, a novel inhibitor of WNT/beta-catenin signaling, in hepatocellular carcinoma (Abstract), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of WNT/b-catenin signaling, is down-regulated in hepatocellular carcinoma: involvement of promoter hypermethylation, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of the WNT/beta-catenin signaling, is frequently downregulated in hepatocellular carcinoma: involvement of methylation-mediated gene silencing, Oncogene. 2005, 24(9): 1607-1614.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), Asian Journal of Surgery. 2005, 28(Suppl 2): S84.

Yau T.O., Chan P.C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), The 4th International Meeting on Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, 14-16 December 2004.

Researcher : Leung BSY

List of Research Outputs

Cheung A.N.Y., Chiu P.M., Tsun O.K.L., Khoo U.S., Leung B.S.Y. and Ngan H.Y.S., Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology, Journal of Clinical Pathology. 2004, 57: 721-727.

Researcher : Leung CY

List of Research Outputs

Chan Y.K., Liu S., Leung C.Y., Cheung A.N.Y., Khoo U.S., Leung T.W. and Ngan H.Y.S., Promoter methylation of Delta-TA isoform of p73 regulating the corresponding transcript expression level in gynecological cancers, 70th Cold Spring Harbor Laboratory Symposium: Molecular Approaches to Controlling Cancer, New York, USA, June 1-6, 2005.

Mok C.C., Ying K.Y., Tang S.C.W., Leung C.Y., Lee K.W., Ng W.L., Wong R.W.S. and Lau W.C.S., Predictors and outcome of renal flares after successful cyclophosphamide treatment for diffuse proliferative lupus glomerulonephritis. , Arthritis & Rheumatism. 2004, 50: 2559-2568.

Researcher : Leung KFS

List of Research Outputs

Mok T.M.Y., Chan E.Y.T., Fong D.Y.T., Leung K.F.S., Wong W.S. and Lau W.C.S., Antiphospholipid Antibody Profiles and Their Clinical Associations in Chinese Patients with Systemic Lupus Erythematosus, The Journal of Rheumatology. 2005, 32 (4): 622-8.

Researcher : Leung SY

Project Title:	Identification of novel differentially expressed genes in gastric cancer and their functional characterisation
Investigator(s):	Leung SY, Yuen ST, Chu KM, Kung H, Lin MC
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2001
Completion Date:	12/2004

Abstract:

To identify and isolate partial cDNA sequences of genes that are differentially-expressed in various stages of gastric carcinogenesis using combined supression subtractive hybridisation and cDNA microarrays; to study cloning of full length cDNA sequences for novel genes; to study expression analysis of these genes in the mRNA and protein level in a large panel of gastric cancers, and examine their relationaship with clinico-pathological parameters, association with aetiological agents, molecular genetic changes, prognosis, response to treatment and their potential use as tujour markers; to study functional characterisation of these novel genes, with regard to their effects in cell division, mitotic activity, tranforming activity, tumourigenicity, roles in cell cycle control, apoptosis, and relationship with other signal trnsduction pathways.

Project Title:	High resolution mapping of chromosomal aberrations by cDNA microarray-based comparative genomic hybridisation and their correlation with gene expression profile of gastric adenocarcinoma
Investigator(s):	Leung SY, Yuen ST, Chu KM, Lin MC, Chen X., So S.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2002

Abstract:

The project attempts to achieve a high resolution mapping of the chromosomal gains and losses in primary gastric cancer samples by hybridisation of genomic DNA to a microarray containing 44,000 human cDNA clones. The changes of DNA copy number will then be correlated with gene expression profiles already available in the gastric cancer samples, generated using the same cDNA microarray. New oncogenes or tumour suppressor genes may be identified in these regions that may constitute new targets for diagnosis, prognostication and pathway specific therapeutic strategy.

Project Title:	Identification of BRAF mutation in various stages of colorectal carcinogenesis and its relationship with KRAS mutation
Investigator(s):	Leung SY, Yuen ST
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002

Abstract:

To examine the biological relationship of BRAF mutation with KRAS mutation. The association of mutations in these two genes with clinico-pathological features, molecular parameters and prognosis will be sought.

Project Title:	Delineation of prognostic biomarkers in gastric cancer using cDNA microarray data, validation in independent dataset and their functional characterisation
Investigator(s):	Leung SY, Yuen ST, Chu KM, Kung H, Lin MC, Chen X., So S.
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	11/2003

Abstract:

To identify a list of genes that significantly predict tumour behaviour and patient outcome in a cohort of 90 gastric adenocarcinoma patients studied by cDNA microarray; to validate the prognostic significance of the top 50 survival genes in a large independent cohort of gastric adenocarcinoma patients using a combination of real-time quantitative RT-PCR, in-situ hybridisation and immunohistochemistry performed in high-density tissue microarray; to carry out functional characterisation of the survival genes using various cell culture and animal models.

Project Title:	Delineation of prognostic biomarkers in gastric cancer using cDNA microarray data, validation in independent dataset and their functional characterisation
Investigator(s):	Leung SY, Yuen ST, Chu KM, Kung H, Lin MC, Chen X., So S.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	11/2003

Abstract:

Project Title:	Detail study of relationship of BRAF and KRAS mutation with microstatellite instability in colorectal cancer
Investigator(s):	Leung SY, Yuen ST
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To examine in detail the incidence of BRAF and KRAS mutation in a large series of MSI colorectal cancer with detail characterization of germine MMR gene mutation and MLH1 promoter methylation.

Project Title:	Genomic screening for potential tumour suppressors silenced by promoter hypermethylation in gastric adenocarcinomas using cDNA microarray
Investigator(s):	Leung SY
Department:	Pathology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

Project Title:	Genomic screening for potential tumour suppressors silenced by promoter hypermethylation in gastric adenocarcinomas using cDNA microarray
Investigator(s):	Leung SY, Yuen ST, Chu KM, Fung TWK, Lin MC, Chen X
Department:	Pathology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005

Abstract:

Gastric cancer, being the second most common cancer worldwide, continues to present with poor prognosis and obscure cause. In view of the relatively high regional incidence in Hong Kong and certain parts of China, our group has initiated an extensive study in gastric cancer using genomics approach in order to build a solid foundation for on-going research. In collaboration with Stanford University (California, U.S.A.), we have reported the gene expression profiles of 126 gastric adenocarcinomatous and non-neoplastic mucosal samples using a cDNA microarray that contains more than 30,000 unique genes. Noted from the expression profiles were around one thousand genes that were significantly down-regulated in gastric tumours compared with the non-neoplastic mucosae. Among these are many potential tumour suppressors which may contribute to the development of gastric cancer. Recent focus on epigenetics has revealed DNA methylation at the gene promoter region as a key mechanism in silencing tumour suppressor genes and hence plays an important role in carcinogenesis. The latest development of genomics approach has also allowed the systematic profiling for genes that are silenced by methylation in tumour cell lines. The data generated can then be analysed in comparison to the gene expression profiles of tumour tissue samples. This approach will lead to the identification of a comprehensive list of genes that are down-regulated in gastric tumour tissues and at the same time, re-expressed in tumour cell lines after demethylation treatment. Aims:1. To identify genes that are induced after demethylation treatment in a large panel of gastric cancer cell lines by cDNA microarray analysis2. To analyse the data against the expression profile database of gastric tumour tissue samples, so as to prioritise a list of genes that are down-regulated in the largest proportion of gastric tumour tissues and at the same time, induced after demethylation treatment in the largest number of gastric cancer cell lines3. To verify the association of promoter demethylation with gene re-expression in selected gastric cancer cell lines by bisulphite genomic sequencing/Pyrosequencing and RT-PCR4. To systematically determine the promoter methylation pattern of these target genes in gastric tumour tissues, non-neoplastic gastric mucosae and peripheral blood leucocytes, and to correlate the extent of methylation with gene expression levels in gastric tumour tissues.5. To correlate the promoter methylation-induced silencing of specific target genes with clinico-pathological parameters, response to treatment and patient outcome

List of Research Outputs

Au W.Y., Mak W., Ho S.L., Leung S.Y. and Kwong Y.L., Reversible paraneoplastic neuropathy associated with T-cell large granular lymphocyte leukemia, Neurology. 2004, 63(3): 588-589.

Cheung Y.T., He Q., Li M., Leung S.Y., Chiu J., Lin M.C. and Kung H., Characterization of human cell cycle related kinase in glioblastoma carcinogenesis by a proteomic study, Advances in Proteomics in Cancer Research, Florida, USA, October 1,2004.

He Q., Cheung Y.H., Leung S.Y., Yuen S.T., Chu K.M. and Chiu J., Diverse proteomic alterations in gastric adenocarcinoma, Proteomics. 2004, 4(10): 3276-3287.

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Ko S., Luk J.M.C., Wong W.Y., Leung S.Y. and Chu K.M., CDX2 co-localizes with liver-intestine cadherin in intestinal metaplasia and adenocarcinoma of stomach, 6th International Gastric Cancer of Congress, Yokohama, Japan, 4-7 May 2005.

Ko S., Chu K.M., Luk J.M.C., Wong W.Y., Yuen S.T., Leung S.Y. and Wong J., CDX2 co-localizes with liver-intestine cadherin in intestinal metaplasia and adenocarcinoma of stomach, Journal of Pathology . 2005, 205: 615-622.

Leung P.W., Lee C.C., Hung S.F., Ho T.P., Tang C.P., Kwong S.L., Leung S.Y., Yuen S.T., Mak-Lieh F., Oosterlaan J., Grady D., Harxhi A., Ding Y.C., Chi H.C., Flodman P., Schuck S., Spence M.A., Moyzis R. and Swanson J., Dopamine receptor D4 (DRD4) gene in Han Chinese children with attention-deficit/hyperactivity disorder (ADHD): increased prevalence of the 2-repeat allele, American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics. 2005, 133B(1): 54-56.

Leung S.Y., Advances in molecular genetics in colorectal cancer, Hong Kong Sanatorium & Hospital and Stanford University Medical Centre Joint Teleconference Symposium entitled “Management of G.I. Malignancy”, Hong Kong, September 2004.

Leung S.Y., Advances in molecular genetics in colorectal cancer, Invited talk to Asia Pacific Digestive Week, Beijing, China, October 2004.

Leung S.Y., Delineation of prognostic markers by expression profiling in gastric cancer, Inaugural Meeting of the Asia-Pacific Gastric Cancer Consortium Australia, August 2004.

Leung S.Y., Delineation of prognostic markers in gastric cancer by expression profiling, Invited talk to Frontier in Biomedical Research, The University of Hong Kong, Hong Kong, December 2004.

Leung S.Y., Expression profiling and molecular genetics of adenocarcinomas arising from the gastrointestinal tract , The Croucher Advanced Study Institute on “Molecular Genetics Cell Signaling in Cancers” Hong Kong, 17-21 January 2005.

Leung S.Y., Yuen S.T., Chu K.M., Mathy J.A., Li R., Chan A.S.Y., Law S.Y.K., Wong J., Chen X. and So S., Expression profiling identifies chemokine (C-C motif) ligand 18 as an independent prognostic indicator in gastric cancer, Gastroenterology. 2004, 127(2): 457-469.

Leung S.Y., Microsatellite instability and hereditary non-polyposis colorectal cancer syndrome – causes, genetic diagnosis and molecular pathways of cancer development, 1th Annual meeting of the Japanese Familial Cancer Society, Fukushima, Japan, June 2005.

Leung S.Y., Microsatellite instability in colon cancer – causes and molecular pathways of tumour development, 6th Jointed Annual Scientific Meeting of the Hong Kong Society of Gastroenterology, Hong Kong, October 2004.

Leung S.Y., Molecular portraits of gastric cancer – What can we learn from expression profiling, Research seminar delivered in Peter MacCallum Cancer Center, Melbourne, Australia, August 2004.

Leung S.Y., Molecular portraits of gastric cancer – a Hong Kong perspective, Inaugural Meeting of the Asia-Pacific Gastric Cancer Consortium Australia, August 2004.

Leung S.Y., Molecular portraits of gastric cancer, Symposium entitled “Genomics, Proteomics and Therapeutics in Cancer Research” organized by Research Centre of Cancer, The University of Hong Kong, Hong Kong, October 2004.

Li V.S.W., Wong C.W., Chan T.L., Chan A.S.W., Zhao W., Chu K.M., Chen X., Yuen S.T., Leung S.Y. and So S., Mutations of PIK3CA in gastric adenocarcinoma, BioMed Central Cancer. 2005, 5: 29.

Stephens P., Edkins S., Davies H., Greenman C., Cox C., Hunter C., Bignell G., Teague J., Smith R., Stevens C., O'Meara S., Parker A., Tarpey P., Avis T., Barthorpe A., Brackenbury L., Buck G., Butler A., Clements J., Cole J., Dicks E., Edwards K., Forbes S., Gorton M., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jones D., Kosmidou V., Laman R., Lugg R., Menzies A., Perry J., Petty R., Raine K., Shepherd R., Small A., Solomon H., Stephens Y., Tofts C., Varian J., Webb A., West S., Widaa S., Yates A., Brasseur F., Cooper C.S., Flanagan A.M., Green A., Knowles M., Leung S.Y., Looijenga L.H., Malkowicz B., Pierotti M.A., Teh B., Yuen S.T., Nicholson A.G., Lakhani S., Easton D.F., Weber B.L., Stratton M.R., Futreal P.A. and Wooster R., A screen of the complete protein kinase gene family identifies diverse patterns of somatic mutations in human breast cancer, Nature Genetics. 2005, 37(6): 590-592.

Stephens P., Hunter C., Bignell G., Edkins S., Davies H., Teague J., Stevens C., O'Meara S., Smith R., Parker A., Barthorpe A., Blow M., Brackenbury L., Butler A., Clarke O., Cole J., Dicks E., Dike A., Drozd A., Edwards K., Forbes S., Foster R., Gray K., Greenman C., Halliday K., Hills K., Kosmidou V., Lugg R., Menzies A., Perry J., Petty R., Raine K., Ratford L., Shepherd R., Small A., Stephens Y., Tofts C., Varian J., West S., Widaa S., Yates A., Brasseur F., Cooper C.S., Flanagan A.M., Knowles M., Leung S.Y., Louis D.N., Looijenga L.H., Malkowicz B., Pierotti M.A., Teh B., Chenevix-Trench G., Weber B.L., Yuen S.T., Harris G., Goldstraw P., Nicholson A.G., Futreal P.A., Wooster R. and Stratton M.R., Lung cancer: intragenic ERBB2 kinase mutations in tumours, Nature. 2004, 431(7008): 525-526.

Subramanian S., West R.B., Corless C.L., Ou W., Rubin B.P., Chu K.M., Leung S.Y., Yuen S.T., Zhu S., Hernandez-Boussard T., Montgomery K., Nielsen T.O., Patel R.M., Goldblum J.R., Heinrich M.C., Fletcher J.A. and van de Rijn M.V.D., Gastrointestinal stromal tumors (GISTs) with KIT and PDGFRA mutations have distinct gene expression profiles, Oncogene. 2004, 2004: 7780-7790.

Tang S.F., Chan K.H., Cheng V.C.C., Woo P.C.Y., Lau S.K.P., Lam C.C.K., Chan T.L., Wu A.K.L., Hung I.F.N., Leung S.Y. and Yuen K.Y., Comparative host gene transcription by microarray analysis early after infection of the Huh7 cell line by severe acute respiratory syndrome coronavirus and human coronavirus 229E., Journal of virology. 2005, 79(10): 6180-93.

Wong C.W., Fan Y.S., Chan T.L., Chan A.S.W., Ho L.C., Ma T.K.F., The Cancer Genome Project , Yuen S.T. and Leung S.Y., BRAF and NRAS mutations are uncommon in melanomas arising in diverse internal organs, Journal of Clinical Pathology. 2005, 58: 640-644.

Xia H.H.X., Yang Y., Lam S.K., Wong R.W.M., Leung S.Y., Yuen S.T., Elia G., Wright N.A. and Wong B.C.Y., Aberrant Epithelial Expression of Trefoil Family Factor 2 and Mucin 6 in Helicobacter pylori infected gastric antrum, incisura, and body and its association with antralisation, Journal of clinical pathology. 2004, 57: 861-866.

Xia H.H.X., Lam S.K., Huang X.R., Wong R.W.M., Leung S.Y., Yuen S.T., Lan H.Y. and Wong B.C.Y., Helicobacter pylori Infection is Associated with Increased Expression of Macrophage Migratory Inhibitory Factor - by Epithelial Cells, T Cells, and Macrophages - in Gastric Mucosa, The Journal of Infectious Diseases. 2004, 190: 293-302.

Researcher : Leung THY

List of Research Outputs

Leung T.H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Identification of multiple isoforms of DLC2, a novel tumor suppressor gene, and their functional characterization in hepatocellular carcinoma, 4th International Meeting of Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, December 2004 (Young Investigator Award). 2004.

Researcher : Li VSW

List of Research Outputs

Li V.S.W., Wong C.W., Chan T.L., Chan A.S.W., Zhao W., Chu K.M., Chen X., Yuen S.T., Leung S.Y. and So S., Mutations of PIK3CA in gastric adenocarcinoma, BioMed Central Cancer. 2005, 5: 29.

Researcher : Li YH

List of Research Outputs

Chiang A.K.S., Ma E.S.K., Jones B.M., Li Y.H., Chan G.C.F., Ha S.Y. and Lau Y.L., Long Term Immune Reconstitution after Bone Marrow Transplantation for Severe Combined Immunodeficiency and Wiskott Aldrich Syndrome, 5th Guangdong - Hong Kong Paediatric Exchange Meeting, HK, 24 July 2004. 第五屆粵港兒科學術交流會，香港， 24 July 2004, 47.

Wan T.S.K., Ma E.S.K., Chow E.Y.D., Li Y.H., Lin S.Y. and Chan L.C., Pathogenesis of jumping translocations: a molecular cytogenetics study, Leukemia Research. 2004, 28(10): 1075-1079.

Researcher : Liong EC

List of Research Outputs

Chen J.H., Tipoe G.L., Liong E.C., So H.S.H., Leung K.M., Tom W.M., Fung P.C.W. and Nanji A.A., Green tea polyphenols prevent toxin-induced hepatotoxicity in mice by down-regulating inducible nitric oxide-derived prooxidants, American Journal of Clinical Nutrition. 2004, 80: 742-751.

Researcher : Liu W

List of Research Outputs

Khoo U.S., Chan Y.K., Peiris J.S.M., Yip S.P., Liu W., Ngan H.Y.S., Tam P.K.H., Chan L.C. and Cheung A.N.Y., Association of ICAM3 genetic variant with Severe Acute Respiratory Syndrome (SARS)., HUGO's 10th Human Genome Meeting, 18-21 April 2005, Kyoto, Japan. 2005.

Liu W., Chan Y.K., Chan S.Y., Yip S.P., Cheung A.N.Y., Chua D.T.T., Ngan H.Y.S. and Khoo U.S., BRCA1 gene promoter polymorphisms associated with breast cancer risk, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Researcher : Lo CK

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Ma L., Chan K.W., Trendell-Smith N.J., Lo C.K., To K.W. and Huang F., Necrotic cells induce systemic autoimmune disease in vivo by activation of dendritic cells, 62th Annual Meeting, American Academy of Allergy, Asthma & Immunology (AAAAI), San Antonio, FL, USA, 3-7 March 2005.

Researcher : Lo KC

List of Research Outputs

Lu L., Lam Q.L.K., Zhang M., Lo K.C., Ko K.H., Osmond D.G., Wu G.E. and Rottapel R., Novel function of c-Abl in regulating precursor B cell development, The 3rd Congress of the Federation of Immunology Societies of Asia-Oceania, Hangzhou, China, April 18-22, 2005.

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Researcher : Lo KC

List of Research Outputs

Researcher : Loong F

List of Research Outputs

Au W.Y., Ma S.Y., Chim J.C.S., Choy C., Loong F., Lie A.K.W., Lam C.C.K., Leung A.Y.H., Tse E.W.C., Yau C.C., Liang R.H.S. and Kwong Y.L., Clinicopathologic features and treatment outcome of mature T-cell and natural killer-cell lymphomas diagnosed according to the World Health Organization classification scheme: a single center experience of 10 years, Annals of Oncology. 2005, 16(2): 206-214.

Au W.Y., Gascoyne R.D., Klasa R.D., Connors J.M., Gallagher R.P., Le N.D., Loong F., Law C.K. and Liang R.H.S., Incidence and spectrum of non-Hodgkin lymphoma in Chinese migrants to British Columbia, British Journal of Haematology. 2005, 128: 792-196.

Chim J.C.S., Wong K.Y., Loong F. and Srivastava G., Absence of ATM hypermethylation in mantle cell and follicular lymphoma, Leukemia. 2005, 19(5): 880-2.

Chim J.C.S., Ma E.S.K., Loong F. and Kwong Y.L., Diagnostic cues for natural killer cell lymphoma: primary nodal presentation and the role of in situ hybridisation for Epstein-Barr virus encoded early small RNA in detecting occult bone marrow involvement, Journal of clinical pathology. 2005, 58(4): 443-5.

Chim J.C.S., Loong F., Ma E.S.K., Cheung W.W.W., Chim J.C.S. and Ooi C.G.C., Extramedullary cardiac plasmacytoma presenting with cardiac tamponade, Journal of Clinical Oncology. 2005, 23(13): 3140-3.

Chim J.C.S., Loong F., Leung A.Y.H., Tsang J. and Ooi C.G.C., Primary follicular lymphoma of the small intestine, Leukaemia & Lymphoma. 2004, 45(7): 1463-6.

Chim J.C.S., Ooi C.G.C., Loong F., Au W.Y., Au W.Y. and Lie A.K.W., Side effects and good effects from new chemotherapeutic agents. Bortezomib in primary refractory plasmacytoma, Journal of Clinical Oncology. 2005, 23(10): 2426-8.

Wong R.W.S., Loong F., Ooi C.G.C., Tse T.C. and Chim J.C.S., Primary granulocytic sarcoma of the mediastinum, Leukaemia & Lymphoma. 2004, 45(9):1931-3.

Researcher : Lu L

Project Title:	Pathogenic effects of SARS-associated coronavirus infection on lymphoid system
Investigator(s):	Lu L, Zheng B, Chan LC, Peiris JSM, Ma ESK, Jones BM, Loong F.
Department:	Pathology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To examine the pathological changes of lymphoid organs and the distribution of SARS-CoV infected cells from patients with SARS; to investigate the SARS-CoV trophism in immune cells and characterize the phenotypic and functional changes of viral-infected lymphocytes.

Project Title:	B cell apoptosis and its regulation in autoimmunity
Investigator(s):	Lu L, Kincade PW
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

To study the regulation of B cell development and survival by BLyS and APRIL in mouse bone marrow; to examine B cell apoptosis and its regulation in the development of CIA as a model of RA; to determine if BLyS and/or APRIL participate(s) in the pathogenesis of CIA.

Project Title:	B cell apoptosis and its regulation in autoimmunity
Investigator(s):	Lu L, Kincade PW
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

Project Title:	Specific gene-silencing therapy for rheumatoid arthritis in collagen-induced mice
Investigator(s):	Lu L, Lin MC, Lam D.
Department:	Pathology
Source(s) of Funding:	Seed Funding Programme for Applied Research
Start Date:	09/2003
Completion Date:	08/2004

Abstract:

To construct si-RNA of BLyS and the adenovirus-associated vectors carrying the si-RNA of BLyS (AAV-siRNA-BLyS) to silence BLyS gene expression in primary immune cells; to examine the gene-silencing effect of AAV-siRNA-BLyS on the activation and function of lymphocytes from normal and CIA mice.

Project Title:	Effect of aging on B lymphopoiesis
Investigator(s):	Lu L
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To determine if B cell differentiation and maturation are impaired during aging process; to identify the differentiation stages at which developing B cells are susceptible to the influence of aging; to study the mechanisms underlying the change of B lymphopoiesis associated with aging.

Project Title:	Natural killer cells and the pathogenesis of autoimmunity
Investigator(s):	Lu L, Paige C.J.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	10/2004

Abstract:

To study : (1) NK cell development during the pathogenesis of autoimmune arthritis. (2) NK cell interaction with T and B cells under autoimmune conditions. (3) role of NK cells in the development of autoimmune arthritis.

Project Title:	Specific silencing of novel TNF family cytokine BAFF by small inerfering RNA as a therapeutic approach for multiple myeloma
Investigator(s):	Lu L, Shen L
Department:	Pathology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	12/2004

Abstract:

refer hard copy proposal

Project Title:	Natural killer cells and the pathogenesis of autoimmunity
Investigator(s):	Lu L
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

Project Title:	Functional interactions of dendritic cells and B cells in autoimmunity
Investigator(s):	Lu L, Cao X.T.
Department:	Pathology
Source(s) of Funding:	NSFC/RGC Joint Research Scheme
Start Date:	03/2005

Abstract:

To study: (1) roles of BLyS in regulating DCs and B cell functions in autoimmunity, (2) roles of heat-shock proteins in regulating functions of DCs and B cells, (3) roles of HSPs in regulating autoimmune arthritis development.

List of Research Outputs

Lu L., Apoptosis and its regulation during B cell development, Symposium of Developmental Immunology, The XIXth International Congress of Zoology, Beijing, China, 2004. 2004.

Lu L., Cellular and Molecular Immunology. Chinese Society of Immunology, 2004.

Lu L., Frontiers in Immunology, Tsinghua University, Beijing, Oct 2004. 2004.

Lu L., Zhang M., Cheung C.T., Wong C., Ko K.H., Tsang S.L., Chan L.C. and Sham M.H., Hoxb3 deficiency impairs B lymphopoiesis, 12th International Conference for Immunology, Montreal, Canada, July 18-23, 2004. 2004.

Lu L., Lam Q.L.K., Zhang M., Lo K.C., Ko K.H., Osmond D.G., Wu G.E. and Rottapel R., Novel function of c-Abl in regulating precursor B cell development, The 3rd Congress of the Federation of Immunology Societies of Asia-Oceania, Hangzhou, China, April 18-22, 2005.

Qu D., Zheng B., Yao X., Guan Y., Yuan Z.H., Zhong N.S., Lu L., Xie J. and Wen Y., Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) in mice induced local and serum antibodies., Vaccine. 2005, 23: 924-931.

Zhang M., Ko K.H., Sham M.H. and Lu L., Novel function of HOXB3 gene in regulation B lymphopoiesis , 9th research postgraduate symposium, HKU, December 4, 2004.

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Researcher : Lui MC

List of Research Outputs

Chay G.W., Lui M.C. and Cheung Y.F., Ethnic Differences in Coagulation Factor Abnormalities after the Fontan Procedure, 5th Guangdong - Hong Kong Paediatric Exchange Meeting, HK, 24 July 2004. 2004.

Researcher : Ma ESK

Project Title:	Genetic modifiers of [beta]-thalassaemia phenotype in the Chinese
Investigator(s):	Ma ESK, Tan-Un KC
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	12/2002
Completion Date:	11/2004

Abstract:

The project is to investigate genetic modifiers of [beta]-thalassaemia phenotype in Chinese patients at candidate gene loci responsible for [gamma]-globin chain production, iron metabolism, bilirubin metabolism and collagen synthesis. The information gathered is applicable not only to genetic counselling and prenatal diagnosis, but will also provide prognostic indicators on which management decisions can be based.

Project Title:	Quantification of mutant transcripts in mild and silent [beta]-thalassaemia alleles of the Chinese by real time polymerase chain reaction
Investigator(s):	Ma ESK
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2004

Abstract:

To determine the level of mutant transcripts in these mild and silent [beta]-thalassaemia alleles by real time polymerase chain reaction.

List of Research Outputs

Au W.Y., Hon C., Cheng V.C.C. and Ma E.S.K., Concomitant zoster myelitis and cerebral leukemia relapse after stem cell transplantation, Annals of Hematology. 2004, 84(1): 59-60.

Au W.Y., Ma E.S.K., Lam V.M.S., Chan L.C., Pang A.W.K. and Kwong Y.L., Glucose 6-phosphate dehydrogenase (G6PD) deficiency in elderly Chinese women heterozygous for G6PD variants, American Journal of Medical Genetics Part A. 2004, 129A(2): 208 - 211.

Au W.Y., Kwok J.S.Y., Chu K.M. and Ma E.S.K., Life-threatening cryoglobulinemia in HCV negative Southern Chinese and a novel association with structural aortic abnormalities, Annals of Hematology. 2005, 84: 95-98.

Au W.Y., Cheng V.C.C., Wan T.S.K. and Ma E.S.K., Myelodysplasia masquerading as parvovirus-related red cell aplasia with giant pronormoblasts, Annals of Hematology. 2004, 83(10): 670-1.

Au W.Y., Liu C.L., Lo C.M., Fan S.T. and Ma E.S.K., Potential role for platelet apheresis for post-liver transplant thrombocytosis complicating portal vein thrombosis, Journal of Clinical Apheresis. 2004, 19(4): 192-196.

Au W.Y., Srivastava G., Wong K.Y., Chung L.P., Ma E.S.K., Wan T.S.K. and Kwong Y.L., Transformation of diffuse large B-cell lymphoma into pre-B acute lymphoblastic leukemia: clinicopathologic features and clonal relationship, Human Pathology. 2004, 35(7): 900-903.

Chan L.C., Chan A.S.Y., Hui E.C., Ha S.Y. and Ma E.S.K., Hb H Disease: Time to Screen?, Genetics and Population Health. Inaugural Conference of the Australian Public Health Genetics Consortium. 2004.

Chay G.W., Ma E.S.K. and Cheung Y.F., Ethnic Differences in Coagulation Factor Abnormalities After the Fontan Procedure, Ruby Jubilee Scientific Meeting, HK, 24-26 Sepember 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 39.

Chen J.F., Ma E.S.K., Ha S.Y., Chan G.C.F., Chan A.Y.Y., Chan L.C. and Lau Y.L., The Effect of Mild b-Thalassaemia Mutations on Clinical Manifestation of b-Thalassaemia Major and Intermedia in Chinese, Ruby Jubilee Scientific Meeting, HK, 24-26 September 2004 (Abstract in Hong Kong Journal of Paediatrics (new series). Hong Kong, Medcom Limited, 2004, 9 (suppl): 18.

Chen J.F., Ma E.S.K., Ha S.Y., Chan G.C.F., Chan A.Y.Y., Chan L.C. and Lau Y.L., The Effect of Mild b-thalassaemia Mutations on Clinical Mainfestation of b-thalassaemia Major and Intermedia in Chinese, 5th Guangdong - Hong Kong Paediatric Exchange Meeting, HK, 24 July 2004. 2004.

Cheung E.W.Y., Chay G.W., Ma E.S.K. and Cheung Y.F., Systemic Oxygen Saturation and Coagulation Factor Abnormalities Before and After the Fontan Procedure, 13^th Annual Scientific Congress of Hong Kong College of Cardiology (abstract published in the Journal of the Hong Kong College of Cardiology), Hong Kong, 22-24 April 2005. 13: 53.

Chiang A.K.S., Ma E.S.K., Jones B.M., Li Y.H., Chan G.C.F., Ha S.Y. and Lau Y.L., Long Term Immune Reconstitution after Bone Marrow Transplantation for Severe Combined Immunodeficiency and Wiskott Aldrich Syndrome, 5th Guangdong - Hong Kong Paediatric Exchange Meeting, HK, 24 July 2004. 第五屆粵港兒科學術交流會，香港， 24 July 2004, 47.

Chim J.C.S., Ma E.S.K., Loong F. and Kwong Y.L., Diagnostic cues for natural killer cell lymphoma: primary nodal presentation and the role of in situ hybridisation for Epstein-Barr virus encoded early small RNA in detecting occult bone marrow involvement, Journal of clinical pathology. 2005, 58(4): 443-5.

Chim J.C.S. and Ma E.S.K., Eosinophilic leukemic transformation in polycythemia rubra vera (PRV), Leukaemia & Lymphoma. 2005, 46(3): 447-50.

Chim J.C.S., Loong F., Ma E.S.K., Cheung W.W.W., Chim J.C.S. and Ooi C.G.C., Extramedullary cardiac plasmacytoma presenting with cardiac tamponade, Journal of Clinical Oncology. 2005, 23(13): 3140-3.

Chung B.H.Y., Ma E.S.K., Khong P.L. and Chan G.C.F., Genetic thrombophilic risk factors in Chinese children with malignant illness, The 3^rd Hong Kong Medical Genetics Conference, HK< 8-10 April 2005. 23.

Ding C., Chiu R.W.K., Lau T.K., Leung T.N., Chan L.C., Chan A.Y.Y., Charoenkwan P., Ng I.S.L., Law H.Y., Ma E.S.K., Xu X., Wanapirak C., Sanguansermsri T., Liao C., Ai M.A.T.J., Chui D.H.K., Cantor C.R. and Lo Y.M.D., MS analysis of single-nucleotide differences in circulating nucleic acids: Application to noninvasive prenatal diagnosis, Proceedings of the National Academy of Sciences. 2004, 101(29): 10762-10767.

Ho M.H.K., Ha S.Y., Chan G.C.F. and Ma E.S.K., Leukemia or Leukemoid, Down Syndrome or not?, Haematologica. 2004, 89(9): ECR33.

Hon C., Ma E.S.K., Yau K. and Au W.Y., CNS Manifestations of Malignancies CASE 3. Leukemic Optic Neuropathy Complicating Leukemia Cutis , Journal of Clinical Oncology. 2005, 23(18): 4229-4230.

Hon C., Ho S.L., Ma E.S.K., Trendell-Smith N.J. and Au W.Y., High-grade lymphoma after azathioprine treatment for Vogt-Kaganayi-Harada syndrome, Leukemia & Lymphoma . 2005, 46: 289-292.

Hon C., Ma E.S.K. and Au W.Y., Unusual Sites of Metastatic Malignancy - CASE 3. Acute Leukemia Presenting As Bilateral Proptosis , Journal of Clinical Oncology. 2004, 22(24): 5015-5016.

Leung K.Y., Lee C.P., Tang M.H.Y., Lau E.T., Ng L.K.L., Lee Y.P., Chan H.Y., Ma E.S.K. and Chan V.N.Y., Cost-effectiveness of prenatal screening for thalassaemia in Hong Kong, Prenatal Diagnosis. 2004, 24: 899-907.

Ma E.S.K., Wan T.S.K. and Chan L.C., FISHing - Current status and future prospects for improved management of leukemia, Cancer Reviews: Asia-Pacific. 2004, 2(2): 131-141.

Ting J.Y., Ma E.S.K. and Wong K.Y., A case of severe haemolytic disease of the newborn due to anti-Di^a antibody, Hong Kong Medical Journal. 2004, 10(5): 347-349.

Wan T.S.K., Ma E.S.K., Chan G.C.F. and Chan L.C., Investigation of MYCN status in neuroblastoma by fluorescence in situ hybridization, International Journal of Molecular Medicine. 2004, 14: 981-987.

Wan T.S.K., Ma E.S.K., Chow E.Y.D., Li Y.H., Lin S.Y. and Chan L.C., Pathogenesis of jumping translocations: a molecular cytogenetics study, Leukemia Research. 2004, 28(10): 1075-1079.

Wong W.H.S., Ma E.S.K., Lee T.L., Ha S.Y., Lau Y.L. and Chan G.C.F., Use of midazolam and ketamine as sedation for children undergoing minor operative procedures., Support Care Cancer. 2005, 13: 1001-1009.

Researcher : Ma L

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Ma L., Ng M.H. and Huang F., Interactions of dendritic cells and dying cells in Systemic Lupus Erythematosus, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Ma L., Chan K.W., Trendell-Smith N.J., Lo C.K., To K.W. and Huang F., Necrotic cells induce systemic autoimmune disease in vivo by activation of dendritic cells, 62th Annual Meeting, American Academy of Allergy, Asthma & Immunology (AAAAI), San Antonio, FL, USA, 3-7 March 2005.

Researcher : Man MLS

List of Research Outputs

Chan Q.K.Y., Khoo U.S., Chan Y.K., Ngan H.Y.S., Li S.S., Chiu P.M., Man M.L.S., Ip P.P.C., Xue W. and Cheung A.N.Y., Promoter methylation and differential expression of p-Class glutathione S-transferase in endometrial carcinoma, The Journal of Molecular Diagnostics. 2005, 7(1): 8-16.

Fong P.Y., Xue W., Ngan H.Y.S., Chan Y.K., Khoo U.S., Tsao G.S.W., Chiu P.M., Man M.L.S. and Cheung A.N.Y., Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome, Cancer. 2004, 103(2): 268-276.

Researcher : Nanji AA

List of Research Outputs

Leung T.M., Fan S.T., Liong E.C., Fung M.L., Lau T.Y.H., Leung K.M., Tom W.M., Nanji A.A. and Tipoe G.L., Nitric oxide and fibrotic factors in chronic liver injury (Abstract), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Zhang H.Y., Nanji A.A., Luk J.M.C., Huang X.R., Lo C.M., Chen Y.X., Yuen S.T., Lan H.Y. and Lau G., Macrophage migration inhibitory factor expression correlates with inflammatory changes in human chronic hepatitis B infection, Liver International. 2005, 25(3): 571-579.

Researcher : Ng IOL

Project Title:	Identification and characterization of DLC-2, a candidate tumour suppressor gene on 13q frequently deleted in liver cancer
Investigator(s):	Ng IOL, Jin D
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2001

Abstract:

To clone the full-length cDNA and genomic sequences of human DLC-2; to determine the accurate chromosomal localizatioin of DLC-2; to analyze the expression pattern of DLC-2 mRNA and protein in tissues and cells; to define the genotypic and phenotypic alterations of DLC-2 in HCC and hepatoma cell lines; to test the RhoGAP and tumour supressor activities of DLC-2; to characterize the signaling pathways as related to DLC-2 and cell transformation; to assess the clinical and pathological significance of DLC-2 in human HCC.

Project Title:	Dysregulation of NF-[kappa]B signaling in liver cancer
Investigator(s):	Ng IOL, Yau TO, Jin D
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2002

Abstract:

The project attempts to document systematically the dyregulation of the important cellular pathway in liver cancer and identify its clinical significance in patient management.

Project Title:	Chimerism in transplanted liver
Investigator(s):	Ng IOL
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To evaluate the chimerism in the transplanted livers in patients after liver transplantation; to delineate cell dynamics, viz. differentiation (into hepatocytes or other cells) of recipient cells, i.e. of extrahepatic origin, in the transplanted liver grafts.

Project Title:	Functional characterization of novel genes in liver cancer
Investigator(s):	Ng IOL, Wan D.F., Gu J.R.
Department:	Pathology
Source(s) of Funding:	NSFC/RGC Joint Research Scheme
Start Date:	01/2003

Abstract:

To define the genetic and phenotypic alterations of the 3 novel genes (LASS2, PP753 and SP192) in human HCC samples and HCC cell lines; to assess the tumour suppressor functions and the signaling pathways of these genes in HCC cells; to evaluate the clinicopathological and prognostic significance of the genes in human HCC.

Project Title:	Study on the pathogenesis of SARS coronavirus by establishing transgenic mouse lines expressing SARS-CoV viral proteins
Investigator(s):	Ng IOL, Yau TO, Sham MH, Chan DW, Peiris JSM, Jin D, Huang F, Poon LLM
Department:	Pathology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To develop transgenic mouse lines that can express the viral proteins of the SARS-coronavirus (CoV) in an inducible form, in tissue-specific and in tissue-nonspecific manner.

Project Title:	Deciphering dysregulation of mitotic checkpoint control in liver cancer
Investigator(s):	Ng IOL, Jin D
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

1) To document and characterize dysregulation of mitotic checkpoint in hepatocellular carcinoma (HCC) by evaluating; a) mitotic checkpoint competence in HCC cell lines, b) expression and alterations of mitotic checkpoint genes and proteins in HCC cells and tissues, c) epigenetic causes of mitotic checkpoint defects in HCC; 2) to investigate the roles of hepatitis B virus-encoded X protein (HBx) in mitotic checkpoint in HCC; 3) to assess the clinical, pathological and prognostic significance of mitotic checkpoint dysfunction in HCC.

Project Title:	Deciphering dysregulation of mitotic checkpoint control in liver cancer
Investigator(s):	Ng IOL, Jin D
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

Project Title:	Characterization of T-cadherin in hepatocellular carcinoma
Investigator(s):	Ng IOL, Chan DW
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003

Abstract:

To evaluate the expression of T-cadherin at mRNA and protein levels in human HCCs and in HCC cell lines; to investigate the factors which induce expression of T-cadherin in HCC.

Project Title:	Functional characterization of DLC1 gene, a novel tumour suppressor gene frequently deleted in liver cancer
Investigator(s):	Ng IOL, Yau TO, Sham MH, Jin D, Ching YP
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To develop and characterize DLC-null mice using gene targeting technology; to delineate the interaction between DLC1 and the downstream effector, ROCK in HCC cell lines and human HCCs.

Project Title:	Characterization of DLC1 gene, a novel tumour suppressor gene frequently deleted in liver cancer
Investigator(s):	Ng IOL, Jin D, Yam JWP, Ching YP
Department:	Pathology
Source(s) of Funding:	Michael Kadoorie Cancer Genetics Research Fund
Start Date:	01/2005

Abstract:

To search for binding partners of DLC1; to delineate the interaction between DLC1 and the downstream effector, ROCK, in HCC cell lines and human HCCs; to assess the clinicopathologic significance of ROCK and its relationship with DLC1 in HCCs.

Project Title:	Characterization of the dishevelled gene in Wnt/[beta]-catenin signaling in liver cancer
Investigator(s):	Ng IOL, Yam JWP, Chan DW
Department:	Pathology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	01/2005

Abstract:

refer hard copy proposal

Project Title:	Genome-wide methylation screening in search for novel tumour suppressor genes in liver cancer
Investigator(s):	Ng IOL, Ching YP, Wong CM
Department:	Pathology
Source(s) of Funding:	Michael Kadoorie Cancer Genetics Research Fund
Start Date:	01/2005

Abstract:

To characterize HCC-specific hypermethylation profile in HCC cells using microarray approach; to identify novel tumour suppressor genes in HCC cells; to delineate epigenetic and genetic alterations of selected putative tumour suppressor genes in human HCC; to characterize tumour suppressor activities of selected novel tumour suppressor genes.

Project Title:	Functional characterization of DLC1 gene, a novel tumour suppressor gene frequently deleted in liver cancer
Investigator(s):	Ng IOL
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Abdalla E.K., Pawlik T.M., Poon R.T.P., Zorzi D., Ikai I., Curley S.A., Nagorney D.M., Belghiti J., Ng I.O.L., Yamaoka Y., Lauwers G.Y. and Vauthey J.N., Critical appraisal of the clinical and pathological predictors of survival after resection of large hepatocellular carcinoma (Abstract), Asian Journal of Surgery. 2005, 28(Suppl): S87.

Abdalla E.K., Pawlik T.M., Poon R.T.P., Sarmiento J.M., Ikai I., Curley S.A., Nagorney D.M., Belghiti J., Ng I.O.L., Yamaoka Y., Lauwers G.Y. and Vauthey J.N., Hepatitis serology defines tumor and liver disease characteristics but not prognosis after resection of hepatocellular carcinoma (Abstract), Asian Journal of Surgery. 2005, 28(Suppl): S81.

Chan D.W., Lee M.F., Chan P.C.Y. and Ng I.O.L., T-cadherin is silenced by frequent genetic and epigenetic alterations in hepatocellular carcinoma, The 11th HK International Cancer Congress, 10-12 November 2004, Hong Kong. 2004.

Chan K.L., Guan X.Y. and Ng I.O.L., High-throughput tissue microarray analysis of c-myc activation in chronic liver diseases and hepatocellular carcinoma, Human Pathology. 2004, 35(11): 1324-1331.

Cheung S.T., Leung K.L., Ip Y.C., Chen X., Fong D.Y.T., Ng I.O.L., Fan S.T. and So S., Claudin-10 expression level is associated with recurrence of primary hepatocellular carcinoma, Clinical Cancer Research. 2005, 11(2 Pt 1): 551-556.

Cheung S.T., Wong S.Y., Leung K.L., Chen X., So S., Ng I.O.L. and Fan S.T., Granulin-epithelin precursor (GEP) promotes growth and invasion of hepatocellular carcinoma (Abstract), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Cheung S.T., Wong S.Y., Leung K.L., Chen X., So S., Ng I.O.L. and Fan S.T., Granulin-epithelin precursor overexpression promotes growth and invasion in hepatocellular carcinoma, Clinical Cancer Research. 2004, 10(22): 7629-7636.

Chin K.T., Zhou H., Wong C.M., Lee M.F., Chan C.P., Qiang B.Q., Yuan J.G., Ng I.O.L. and Jin D., The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma, Nucleic Acids Research. 2005, 33(6): 1859-1873.

Ching Y.P., Wong C.M., Jin D. and Ng I.O.L., Identification of a novel RHO GTPASE activating protein called DLC2, involved in hepatocellular carcinoma, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.

Ho C.C., Siu W.Y., Chow J.P.H., Lau A., Arooz T., Tong H.Y., Ng I.O.L. and Poon R.Y.C., The relative contribution of CHK1 and CHK2 to Adriamycin-induced checkpoint, Experimental Cell Research. 2005, 304(1): 1-15.

Ho J.C.Y., Cheung S.T., Leung K.L., Ng I.O.L. and Fan S.T., Decreased expression of cytochrome P450 2E1 is associated with poor prognosis of hepatocellular carcinoma, International Journal of Cancer. 2004, 111(4): 494-500.

Lai A.T.Y., Lam C.M., Ng K.K.C., Yeung C., Ho W.L., Poon L.T. and Ng I.O.L., Hepatic actinomycosis presenting as a liver tumour: Case report and literature review, Asian Journal of Surgery. 2004, 27(4): 345-347.

Lee K.W., Man K., Poon R.T.P., Lo C.M., Ng I.O.L. and Fan S.T., Disruption of p53-p21/WAF1 cell cycle pathway contributes to progression and worse clinical outcome of hepatocellular carcinoma, Oncology Reports. 2004, 12(1): 25-31.

Lee K.W., Man K., Ho J.W.Y., Sun K.W., Ng T.P., Wang X., Wong Y.C., Ng I.O.L., Xu R. and Fan S.T., FTY720 induces apoptosis of human hepatoma cell lines through PI3-K-mediated Akt dephosphorylation, Carcinogenesis. 2004, 25(12): 2397-2405.

Lee K.W., Man K., Ho J.W.Y., Wang X., Poon R.T.P., Sun K.W., Ng T.P., Ng I.O.L., Xu R. and Fan S.T., Significance of the Rac signaling pathway in HCC cell motility: implications for a new therapeutic target, Carcinogenesis. 2005, 26(3): 681-687.

Leung T.H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Identification of multiple isoforms of DLC2, a novel tumor suppressor gene, and their functional characterization in hepatocellular carcinoma, 4th International Meeting of Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, December 2004 (Young Investigator Award). 2004.

Li H.Y., Fung K.L., Ching Y.P., Ng I.O.L., Chung S.S.M., Sze K.H., Ko B.C.B. and Sun H., Structural study of the SAM domain of the deleted in liver cancer 2 (DLC2), Chemistry Symposium of Hong Kong, March 2005.

Liu C.L., Fan S.T., Lo C.M., Chan S.C., Tso W.K., Ng I.O.L. and Wong J., Hepatic resection for incidentaloma, Journal of Gastrointestinal Surgery. 2004, 8(7): 785-793.

Lo C.M., Fan S.T., Liu C.L., Yong B.H., Wong Y., Lau G., Lai C.L., Ng I.O.L. and Wong J., Lessons learned from one hundred right lobe living donor liver transplants, Annals of Surgery. 2004, 240(1): 151-158.

Lo C.M., Liu C.L., Lau G., Chan S.C., Ng I.O.L., Fan S.T. and Wong J., Liver transplantation for chronic hepatitis B with lamivudine-resistant YMDD mutant using add-on adefovir dipivoxil to lamivudine (Abstract), American Journal of Transplantation. 2005, 5(Suppl): 180.

Lo C.M., Liu C.L., Lau G., Chan S.C., Ng I.O.L., Fan S.T. and Wong J., Liver transplantation for chronic hepatitis B with lamivudine-resistant YMDD mutant using add-on adefovir dipivoxil to lamivudine (Poster Presentation), American Transplant Congress, Seattle, U.S.A., 21-25 May 2005.

Lo C.M., Fan S.T., Liu C.L., Chan S.C., Ng I.O.L. and Wong J., Living donor versus deceased donor liver transplantation for early unresectable hepatocellular carcinoma: same criteria, different outcome (Abstract), HPB. 2005, 7(1 Suppl): 54.

Lo C.M., Fan S.T., Liu C.L., Chan S.C., Ng I.O.L. and Wong J., Living donor versus deceased donor liver transplantation for small unresectable hepatocellular carcinoma: same criteria, different outcome (Invited Lecture), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Ma S.Y., Au W.Y., Lie A.K., Ng I.O.L., Leung A.Y.H., Tse E.W.C., Liang R.H.S., Lau G. and Kwong Y.L., Liver graft-versus-host disease after donor lymphocyte infusion for relapses of hematologic malignacies post allogeneic hematopoietic stem cell transplantation, Bone Marrow Transplant. 2004, 34: 57-61.

Ng D.C.H., Jin D. and Ng I.O.L., Characterisation of Steroidogenic Acute Regulatory Protein Related Lipid Transfer Domain (START)domain in Deleted in Liver Cancer 2(DLC-2)., Society For Chinese Bioscientists in America, Beijing, July 2004 . 2004.

Ng D.C.H., Ching Y.P., Ng I.O.L. and Jin D., Functional characterization of a Novel RhoGAP protein deleted in liver cancer 2 (DLC2), 9th research postgraduate symposium, HKU, December 4, 2004.

Ng I.O.L., Chimerism in transplant allografts, British Journal of Surgery. 2005, 92(6): 661-662.

Ng I.O.L., Croucher Senior Medical Fellowship 2005-2006, The Croucher Foundation. 2005.

Ng I.O.L., Molecular and genetic analysis of hepatocellular carcinoma, The Croucher Advanced Study Institute on “Molecular Genetics Cell Signaling in Cancers” Hong Kong, 17-21 January 2005.

Ng K.K.C., Vauthey J.N., Pawlik T.M., Lauwers G.Y., Regimbeau J.M., Belghiti J., Ikai I., Yamaoka Y., Curley S.A., Nagorney D.M., Ng I.O.L., Fan S.T. and Poon R.T.P., Is hepatic resection for large or multinodular hepatocellular carcinoma justified? Results from a multi-institutional database (Abstract), Asian Journal of Surgery. 2005, 28(Suppl 2).

Ng K.K.C., Vauthey J.N., Pawlik T.M., Lauwers G.Y., Regimbeau J.M., Belghiti J., Ikai I., Yamaoka Y., Curley S.A., Nagorney D.M., Ng I.O.L., Fan S.T. and Poon R.T.P., Is hepatic resection for large or multinodular hepatocellular carcinoma justified? Results from a multi-institutional database, Annals of Surgical Oncology. 2005, 12(5): 364-373.

Pawlik T.M., Poon R.T.P., Abdalla E.K., Zorzi D., Ikai I., Curley S.A., Nagorney D.M., Belghiti J., Ng I.O.L., Yamaoka Y., Lauwers G.Y. and Vauthey J.N., Critical appraisal of the clinical and pathologic predictors of survival after resection of large hepatocellular carcinoma, Archives of Surgery. 2005, 140(5): 450-457.

Pawlik T.M., Poon R.T.P., Abdalla E.K., Zorzi D., Ikai I., Curley S.A., Nagorney D.M., Belghiti J., Ng I.O.L., Yamaoka Y., Lauwers G.Y. and Vauthey J.N., Critical appraisal of the clinical and pathological predictors of survival after resection of large hepatocellular carcinoma (Abstract), The 112th Scientific Session of the Western Surgical Association; Las Vegas, U.S.A., 9 November, 2004.

Pawlik T.M., Poon R.T.P., Abdalla E.K., Ikai I., Nagorney D.M., Belghiti J., Kianmanesh R., Ng I.O.L., Curley S.A., Yamaoka Y., Lauwers G.Y. and Vauthey J.N., Hepatectomy for hepatocellular carcinoma with major portal or hepatic vein invasion: results of a multicenter study, Surgery. 2005, 137(4): 403-410.

Pawlik T.M., Poon R.T.P., Abdalla E.K., Sarmiento J.M., Ikai I., Curley S.A., Nagorney D.M., Belghiti J., Ng I.O.L., Yamaoka Y., Lauwers G.Y. and Vauthey J.N., Hepatitis serology predicts tumor and liver disease characteristics but not prognosis after resection of hepatocellular carcinoma, Journal of Gastrointestinal Surgery. 2004, 9(7): 794-805.

Poon R.T.P., Ho J.W.Y., Tong C.S.W., Lau C.P.Y., Ng I.O.L. and Fan S.T., Prognostic significance of serum vascular endothelial growth factor and endostatin in patients with hepatocellular carcinoma, British Journal of Surgery. 2004, 91(10): 1354-1360.

Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Association of MAD2 Expression with Mitotic Checkpoint Competence in Hepatoma Cells, J. Biomed. Sci. . 2004, 11: 920-7.

Wong C.M., Ching Y.P. and Ng I.O.L., Genome-wide methylation screening in search for novel tumor suppressor genes in liver cancer, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Frequent down-regulation of HDPR1, a novel inhibitor of WNT/beta-catenin signaling, in hepatocellular carcinoma (Abstract), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of WNT/b-catenin signaling, is down-regulated in hepatocellular carcinoma: involvement of promoter hypermethylation, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of the WNT/beta-catenin signaling, is frequently downregulated in hepatocellular carcinoma: involvement of methylation-mediated gene silencing, Oncogene. 2005, 24(9): 1607-1614.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), Asian Journal of Surgery. 2005, 28(Suppl 2): S84.

Yau T.O., Chan P.C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), The 4th International Meeting on Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, 14-16 December 2004.

Yuen M.F., Ng I.O.L., Fan S.T., Yuan H., Wong D.K.H., Yuen J.C.H., Sum S.M., Chan O.O. and Lai C.L., Significance of HBV DNA levels in liver histology of HBeAg and anti-HBe positive patients with chronic hepatitis B, American Journal of Gastroenterology. 2004, 99(10): 2032-2037.

Researcher : Ng WK

List of Research Outputs

Chan G.S.W., Ng W.K., Nicholls J.M. and Chan K.W., Pathologic quiz case: acute renal failure secondary to an uncommon urinary bladder tumor. Micropapillary transitional cell carcinoma of urinary bladder, Archives of Pathology and Laboratory Medicine. 2005, 129(2): e53–e54.

Researcher : Nicholls JM

Project Title:	Ultrastructural characterization of the SARS coronavirus (SARS-CoV)
Investigator(s):	Nicholls JM, Chan KH
Department:	Pathology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To determine the time course of SARS-CoV assembly in infected FRhK-4 and Vero cells; to analyze the formation of syncitia by SARS-CoV in cell culture; to detect changes in SARS-CoV formation under the effect of chemotherapeutic agents that block microtubule formation; to view the antigenic determinants of antibodies to the SARS-CoV.

List of Research Outputs

Burrows J.M., Bromham L., Woolfit M., Piganeau G., Tellam J., Conolly G., Webb N., Poulsen L., Cooper L., Burrows S., Moss D.J., Haryan S.M., Ng M., Nicholls J.M. and Khanna R., Selection pressure-driven evolution of the Epstein Barr virus encoded oncogene, LMP1, in virus isolates from south-east Asia, Journal of Virology. 2004, 78(13): 7131-7137.

Chan G.S.W., Ng W.K., Nicholls J.M. and Chan K.W., Pathologic quiz case: acute renal failure secondary to an uncommon urinary bladder tumor. Micropapillary transitional cell carcinoma of urinary bladder, Archives of Pathology and Laboratory Medicine. 2005, 129(2): e53–e54.

Fan H., Nicholls J.M., Chua D.T.T., Chan K.H., Sham J.S.T., Lee S. and Gulley M.L., Laboratory Markers Of Tumor Burden In Nasopharyngeal Carcinoma: A Comparison Of Viral Load And Serologic Tests For Epstein-barr Virus, International Journal of Cancer. 2004, 112(6): 1036-41.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine up-regulation in SARS-coronavirus-infected, monocyte-derived human dendritic cells, Blood. 2005, 106(7): 2366-2374.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Chan Y.O., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Chemokine upregulation in SARS coronavirus infected human monocyte derived dendritic cells, Keystone Symposia: Dendritic cells at the Center of Innate and Adaptive Immunity: Eradication of Pathogens and Cancer and Control of Immunopathology, Vancouver, Canada, 1-7 February 2005. 85.

Law H.K.W., Cheung C.Y., Ng I.H.Y., Sia S.F., Luk W., Nicholls J.M., Peiris J.S.M. and Lau Y.L., Human Monocyte Derived Dendritic Cells Infected by SARS Associated Coronavirus, 12th International Congress of Immunology and 4th Annual Conference of FOCIS, Montreal, Canada, 18-23 July 2004.

Poon L.L.M., Guan Y., Nicholls J.M., Yuen K.Y. and Peiris J.S.M., The aetiology, origins, and diagnosis of severe acute respiratory syndrome., Lancet Infectious Diseases. 2004, 4(11): 663-71.

Ren Y., Lin C.L., Li Z., Chen X.Y., Huang X., Lui V.C.H., Nicholls J.M., Lan H.Y. and Tam P.K.H., Up-regulation of macrophage migration inhibitory factor in infants with acute neonatal necrotizing enterocolitis, Histopathology. 2005, 46(6): 659-667.

Researcher : Samaranayake YH

Project Title:	The differential expression of C. albicans phospholipases PLB1, PLB2, PLC1, and PLD1 in different growth media
Investigator(s):	Samaranayake YH, Samaranayake LP
Department:	Faculty of Dentistry
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2004

Abstract:

To determine the differential regulation of the four phospholipase genes (i.e. caPLB1, caPLB2, caPLC1 and caPLD1) in C. albicans grown in both a YPD medium and in the conventional egg-yolk medium; to correlate caPLC1 gene expression with the growth rate of C. albicans.

Project Title:	Genomic basis of fluconazole resistance in C.glabrata
Investigator(s):	Samaranayake YH, Samaranayake LP
Department:	Faculty of Dentistry
Source(s) of Funding:	Small Project Funding
Start Date:	11/2004

Abstract:

To obtain a drug resistant C. glabrata strain by sequential exposure to sub-minimal inhibitory concentrations of fluconazole; to determine cell viability (by ATP measurement) after drug exposure and observe the morphological changes by SEM.

Researcher : Shek TWH

List of Research Outputs

Au W.Y., Chan C., Pang A., Lie A.K.W., Liang R.H.S., Yuen P.W., Shek T.W.H., Kwong Y.L. and Pang A.W.K., Nonhematologic malignancies after allogeneic hematopoietic stem cell transplantation: incidence and molecular monitoring, Bone Marrow Transplantation. 2004, 34: 981-985.

Au W.Y., Lie A.K.W., Liang R.H.S., Yuen P.W., Shek T.W.H. and Kwong Y.L., Secondary non-hematologic malignancies after allogeneic stem cell transplantation : incidence and molecular monitoring, 10th Congress of the International Society of hematology, Asian Pacific Division, Nagoya, Japan. Nagoya, Japan, 2004, 110.

Au W.Y., Lam P. and Shek T.W.H., Uncommon presentations of some common malignancies: Case 2. Nasopharyngeal carcinoma followed by secondary acute promyelocytic leukemia presenting with respiratory distress, Journal of Clinical Oncology. 2005, 23(6): 1314-1315.

Hon C., Law R.W., Shek T.W.H. and Au W.Y., CNS Manifestations of Malignancies CASE 1. Conjunctival Relapse of Acute Lymphoblastic Leukemia Heralding Pituitary and CNS Disease , Journal of Clinical Oncology. 2005, 23(18): 4225-4226.

Hon C., Au W.Y. and Shek T.W.H., Intraocular lymphoma as a masquerade syndrome complicating cerebral lymphoma of the corpus callosum, Annals of Hematology. 2005, 84(3): 203 - 204.

Leung K.Y., Tang M.H.Y., Lam T.P.W., Fan Y.W., Shek T.W.H., Wong K.Y. and Ngai C.S.W., Prenatal diagnosis of a cavernous angioma associated with intracranial hemorrhag: report of one case and review of the literature, Ultrasound in Obstetrics & Gynecology. John Wiley & Sons, Ltd, 2004, 24(7): 797-804.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H., To J.Y.T., Wo Y.H., Ho D.W.Y. and Fan S.T., Comparison of systemic responses of radiofrequency ablation, cryotherapy, and surgical resection in a porcine liver model, Annals of Surgical Oncology. 2004, 11(7): 650-657.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H., Ho D.W.Y. and Fan S.T., Maximal host tolerance to large-volume hepatic radiofrequency ablation in a rat model (Abstract), Journal of Gastrointestinal Surgery. 2004, 8( 7 Suppl): 114A.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H., Ho D.W.Y. and Fan S.T., Maximal host tolerance to large-volume hepatic radiofrequency ablation in a rat model (Abstract), The 19th World Congress of Digestive Surgery, Yokohama, Japan, 8-11 December 2004.

Ng K.K.C., Lam C.M., Poon R.T.P., Shek T.W.H., Yu W.C., To J.Y.T., Wo Y.H., Lau C.P.Y., Tang T.C.M., Ho D.W.Y. and Fan S.T., Porcine liver: morphological characteristics and cell viability at experimental radiofrequency ablation with internally cooled electrodes, Radiology. 2005, 235(2): 478-486.

Szeto C.H., Shek T.W.H., Lie A.K.W., Au W.Y., Yuen P.W. and Kwong Y.L., Squamous cell carcinoma of the tongue complicating chronic oral mucosal graft-versus-host disease after allogeneic hematopoietic stem cell transplantation, American Journal of Hematology. 2004, 77(2): 200-202.

Researcher : Shen L

List of Research Outputs

Srivastava G., Shen L., Au W.Y., Kwong Y.L. and Liang R.H.S., Molecular pathogenesis of nasal NK/T-cell lymphoma, Abstract of the Proc of 63rd Annual Meeting of the Japanese Cancer Association, 29 September - 1 October 2004, Fukuoka, Japan. 2004.

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Researcher : So CKC

List of Research Outputs

Cheung N., So C.W., Yam J.W.P., So C.K.C., Poon R.Y.C., Jin D. and Chan L.C., Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukemia, Biochemical Journal. 2004, 383(Pt 1): 27-35.

Researcher : So HSH

List of Research Outputs

Chen J.H., Tipoe G.L., Liong E.C., So H.S.H., Leung K.M., Tom W.M., Fung P.C.W. and Nanji A.A., Green tea polyphenols prevent toxin-induced hepatotoxicity in mice by down-regulating inducible nitric oxide-derived prooxidants, American Journal of Clinical Nutrition. 2004, 80: 742-751.

Researcher : Srivastava G

Project Title:	Cloning of novel translocation partner genes involving immunoglobulin heavy chain gene locus in gastric lymphoma by long-distance inverse PCR
Investigator(s):	Srivastava G, Liang RHS, Lu L
Department:	Pathology
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2003

Abstract:

To clone the translocation partner genes involving the IGH gene locus in GL by LDI-PCR methods and determine the frequency of these novel translocations in GL by direct PCR on chromosomal DNA; to study the expression of novel IGH translocation partner genes in GL by RT-PCR, ISH and immunostaining to determine whether the over expression of these genes is associated with the IGH translocation of these genes in GL; to determine the mRNA and protein expression of novel IGH fusion partner genes in human B-lineage cell lines that correspond to different stage of B-cell development and in primary B cells in reactive tonsils and lymph node. Loss of the normal control mechanisms regulating expression of these novel IGH partner genes may contribute to malignant transformation in lymphomas; to compare the levels of promoter activities by luciferase assays in COS-7 cells between IGH and the promoter of novel translocation partner genes and investigate by transfection experiments whether these IGH translocations do indeed result in the over expression of the translocation partner genes identified in this study by promoter substitution, due, in part, to the presence of potent B cell-specific transcriptional enhancers wihtin the IGH loci; to dertermine the transforming and tumorigenic potential of each of the overexpressed novel IGH translocation partner genes by in vitro growth properties and in vivo tumorigenicity assays; to analyze the mutations of incoming novel IGH translocation partner genes in GL since in B cells where the somatic hypermutation mechanism is active, mutations of the incoming gene may be observed and may contribute to the neoplastic phenotype; to investigate whether other heterologous promoters may also deregulate the involved genes of IGH translocation by fusion in GL by employing 5' RACE (rapid amplification of cDNA ends) strategy; to correlate the detection of novel IGH translocations with clinco-pathological features.

Project Title:	Study of epigenetic inactivation of ATM gene in mantle cell lymphoma
Investigator(s):	Srivastava G
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2005

Abstract:

To study the importance of methylation of the ATM gene in MCL lymphomagenesis; to study the prognostic impact of ATM methylation in MCL.

Project Title:	Role of CD44 and protein kinase C-binding protein 1, novel translocation partners of immunoglobulin heavy chain gene, in the pathogenesis of gastric lymphoma
Investigator(s):	Srivastava G
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2004

Abstract:

To investigate the role of CD44 and PRKCBP1. For CD44 study: (1) to determine whether the gene amplification of CD44 represents another mechanism of CD44 upregulation in GL cases with strong CD44 expression. (2) to determine whether the hypermethylation of the promoter region of CD44 gene may be responsible for the downregulation of CD44 in GL cases lacking CD44 expression. (3) to determine whether the lack of CD44 mRNA expression detected in the pre- B-cell lines and some mature B-cell lines is due to hypermethylation of the promoter region of CD44 gene and whether the silencing of CD44 in the these cell lines could be partially reversed by the treatment of these cell lines with the demethylation agent 5-aza-2'-deoxycytidine (5-aza-^dC). (4) to detect the somatic mutations in CD44 cDNA and genomic DNA in GL, as the presence of mutations at hyaluronan binding sites of CD44 may affect its normal biological function. (5) to identify different CD44 splicing variants (CD44v) in GL since a correlation between expression of CD44v and adverse prognosis has been reported in several subtypes of human carcinoma and in NHL. (6) to correlate the findings of the IGH/CD44 translocation, gene amplification of CD44, hypermethylation of CD44 promoter, CD44 expression, somatic mutations in CD44 cDNA and the different CD44 splicing variants in GL with the clinico-pathological features (CPC) to define prognostic subgroups. (7) to clone the wild-type cDNA CD44 and CDNA lacking the leader peptide, as the result of JGH/CD44 translocation in GL, and perform the transfection assays for their cellular localization and to determine whether this variant of CD44 without the leader peptide has acquired additional tumor transforming function. For PRKCBPI study: (1)to examine PRKCBP1 mRNA expression in primary B cells by in-situ hybridization in normal lymphoid tissues including reactive tonsil, lymph node and spleen. (2) to examine PRKCBP1 mRNA expression by Northern blot hybridization and RT-PCR in the normal peripheral B-lymphocytes and in human B-lineage tumor cell lines corresponding to different stages of B-cell development. (3) to determine whether the PRKCBP1 mRNA is differentially expressed in the tumor cells of GL. (4) to detect the somatic mutations in PRKCBP1 CDNA sequences are prone to mutations coding (a)₈ microsatellite in PRKCBP1 mRNA. Repetitive DNA sequences are prone to mutations. (5) to correlate the findings of the IGH/PRKCBP1 translocation, differential PRKCBP1 mRNA expression and somatic mutations in PRKCBP1 cDNA in GL with the CPC to define prognostic subgroups. (6) to clone the wild-type PRKCBP1 cDNA lacking the first 11 non-coding exons, as the result of IGH/PRKCBP1 translocation in GL, and perform the transfection studies for the cellular localization of their protein to predict possible function of this gene.

Project Title:	Characterization of the role of immunoglobulin heavy and light chain [kappa] and [lambda] gene translocations in the pathogenesis of gastric lymphoma
Investigator(s):	Srivastava G, Liang RHS
Department:	Pathology
Source(s) of Funding:	Michael Kadoorie Cancer Genetics Research Fund
Start Date:	01/2005

Abstract:

To clone IGH-related translocations in GL at both IGHJ and 5' S[mu] region by LDI-PCR and those involving switch region sequences 3' to the S[mu], S[gamma]1-4, and S[alpha]1-2 regions by LDV-PCR; to clone IG[kappa]- and IG[lambda]-related translocations involving the joining region in GL by LDI-PCR; to determine the frequency of the known and novel IG (IGH, IG[kappa], IG[lambda])-related translocations identified by us in GL by direct PCR on genomic DNA; to study the expression of novel IG translocation partner genes in GL by immunostaining to determine whether the over expression of these genes in the tumor cells is associated with the IG translocation of these genes in GL; to determine the mRNA expression of novel IG translocation partner genes in the normal peripheral B-cells and human B-lineage tumor cell lines that correspond to different stages of B-cell development, loss of the normal control mechanisms regulating expression of these novel IG partner genes may contribute to malignant transformation in lymphomas; to analyze the mutations of incoming novel IG translocation partner genes in GL since in B cells, where the somatic hypermutation mechanism is active, mutations of the incoming gene may be observed and may contribute to the neoplastic phenotype; to determine the transforming and tumorigenic potential of each of the overexpressed novel IG translocation partner genes by in vitro growth properties and in vivo tumorigenicity assays; to determine the prevalence of all the cloned novel IG-related translocations identified by us in GL in nodal specimens (MZBCL and DLBCL) by direct PCR, for comparison of IG translocations in gastric MALT and DLBCL with their respective nodal counterparts.

Project Title:	Role of CD44 and protein kinase C-binding protein 1, novel translocation partners of immunoglobulin heavy chain gene, in the pathogenesis of gastric lymphoma
Investigator(s):	Srivastava G
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Au W.Y., Srivastava G., Wong K.Y., Chung L.P., Ma E.S.K., Wan T.S.K. and Kwong Y.L., Transformation of diffuse large B-cell lymphoma into pre-B acute lymphoblastic leukemia: clinicopathologic features and clonal relationship, Human Pathology. 2004, 35(7): 900-903.

Chim J.C.S., Wong K.Y., Loong F. and Srivastava G., Absence of ATM hypermethylation in mantle cell and follicular lymphoma, Leukemia. 2005, 19(5): 880-2.

Fatima S., Hui K.S., Wong M.M., Tang W.K., Chui C.H., Wong J., Law S.Y.K., Tsao G.S.W., Lam K.Y., Srivastava G., Ho K.P. and Tang J.C.O., Study of transforming capacity of two novel genes JS-1 and JS-2 in chromosome 5p and their overexpression in human esophageal squamous cell carcinoma, 96th Annual Meeting of the American Association for Cancer Research, Anaheim, California, USA, 16-20 April. 2005.

Srivastava G., Epigenetics and Molecular Targeting in Cancer, 11th HK International Cancer Congress, Hong Kong (10 to 12 November, 2004). 2004.

Srivastava G., Epigenetics and molecular targeting in cancer, Abstract of the Proc of the 11th HK International Cancer Congress, 10-12 November 2004, Hong Kong. 2004.

Srivastava G., Molecular Pathogenesis of Nasal NK/T-cell Lymphoma, 63rd Annual Meeting of the Japanese Cancer Association, Fukuoka, Japan (September 29 to October 1, 2004). 2004.

Srivastava G., Shen L., Au W.Y., Kwong Y.L. and Liang R.H.S., Molecular pathogenesis of nasal NK/T-cell lymphoma, Abstract of the Proc of 63rd Annual Meeting of the Japanese Cancer Association, 29 September - 1 October 2004, Fukuoka, Japan. 2004.

Yang L., Leung A.C.C., Ko J.M.Y., Lo P.H.Y., Tang J.C.O., Srivastava G., Oshimura M., Stanbridge E.J., Daigo Y., Nakamura Y., Tang C.M.C., Lau K.W., Law S.Y.K. and Lung M.L., Tumor suppressive role of a 2.4 Mb 9q33-q34 critical region and DEC1 in esophageal squamous cell carcinoma, Oncogene. 2005, 24(4): 697-705.

Yang L.C., Tang J.C.O., Srivastava G., Stanbridge E.J. and Lung M.L., Functional investigation of tumor suppressive role of chromosome 9 in esophageal squamous cell carcinoma, Journal of Clinical Oncology. 2004, 22(14): 852S-852S 9571 Supp. S.

Ying Y., Srivastava G., Hsieh W.S., Gao Z., Murray P.G. and Tao Q., Genomic DNA methylation-subtraction identified functional tumor suppressor genes epigenetically silenced in nasopharyngeal carcinoma (NPC), Abstract of the Proc of 11th Biennial Conference of the Association for research on EBV and Associated Diseases, 20-25 September 2004, Regensburg, Germany. 2004.

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Researcher : Sun Q

List of Research Outputs

Sun Q., Huang F. and Chan L.C., Dendritic cells differentiation from cells of leukemia origin, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Researcher : Sze MF

List of Research Outputs

Sze M.F., Ching Y.P., Jin D. and Ng I.O.L., Association of MAD2 Expression with Mitotic Checkpoint Competence in Hepatoma Cells, J. Biomed. Sci. . 2004, 11: 920-7.

Researcher : Tam IYS

List of Research Outputs

Li S.S., Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Tam I.Y.S., Chiu P.M., Ip P.P.C., Tam K.F. and Cheung A.N.Y., Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: A tissue microarray and clinicopathological analysis, Histopathology. 2005, 46(3): 307-313.

Tam I.Y.S., 2005 AACR-ITO EN, Ltd. Scholar-In-Training AwardPurpose: To foster and enhance the education and training of promising cancer researchers residing in Asian countries, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005. 2005.

Tam I.Y.S., EGFR & K-RAS Mutation Pattern in NSCLC, Respiratory Research Meeting, Queen Mary Hosptial, Hong Kong, 4 April 2005.

Tam I.Y.S., Wong M.P., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., EGFR mutations are prevalent and independent from K-RAS mutations in lung adenocarcinomas from non-smokers, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Tam I.Y.S., Wong M.P., Suen W.S., Wang E., Lam W.K., Chiu S.W., Gazdar A., Minna J.D. and Chung L.P., EGFR mutations are prevalent and independent from k-RAS mutations in lung adenocarcinomas from non-smokers, 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, M.S.CL00.01: 1666.

Wong M.P., Lam D.C.L., Yap D.Y.L., Girard L., Tam I.Y.S., Chung L.P., Chiu S.W., Lam W.K., Danchin A. and Minna J.D., Identification of discriminating gene expression of EBV-associated primary lymphoepitheioma-like carcinoma of lung by oligonucleotide microarray analysis , 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, P.O.CB19: 75/B#3.

Researcher : Tam S

List of Research Outputs

Cheung B.M.Y., Lam T.H., Lam K.S.L., Tam S., Wat N.M.S., Lo L.F., Chau F.Y., Law C.Y., Man Y.B., Cheng C.H., Kumana C.R. and Lau C.P., Follow Up of the Hong Kong Cardiovascular Risk Factor Prevalence Survey Cohort, 9th Medical Research Conference, Faculty of Medicine, The University of Hong Kong 2004.

Cheung B.M.Y., Man Y.B., Lo L.F., Chau F.Y., Law C.Y., Lam K.S.L., Lam T.H., Wat N.M.S., Tam S., Cheng C.H., Kumana C.R. and Lau C.P., High Blood Pressure is Related to Obesity in Hong Kong, 9th Medical Research Conference, Faculty of Medicine, The University of Hong Kong 2004.

Cheung B.M.Y., Man Y.B., Lo L.F., Chau F.Y., Law C.Y., Lam K.S.L., Lam T.H., Wat N.M.S., Tam S., Cheng C.H., Kumana C.R. and Lau C.P., Lipid Profile of the Hong Kong Cardiovascular Risk Factor Prevalence Survey Cohort, 9th Medical Research Conference, Faculty of Medicine, The University of Hong Kong 2004.

Cheung B.M.Y., Man Y.B., Lo L.F., Chau F.Y., Law C.Y., Lam K.S.L., Lam T.H., Wat N.M.S., Tam S., Cheng C.H., Kumana C.R. and Lau C.P., Obesity in the Hong Kong Cardiovascular Risk Factor Prevalence Survey-2 (CRISPS2) Cohort, 9th Medical Research Conference, Faculty of Medicine, The University of Hong Kong 2004.

Cheung B.M.Y., Man Y.B., Lam K.S.L., Wat N.M.S., Lo L.F., Chau F.Y., Law C.Y., Lam T.H., Leung G.M., Tam S., Cheng C.H., Kumana C.R. and Lau C.P., Prevalence of Diabetes Mellitus (DM) in the Hong Kong Cardiovascular Risk Factor Prevalence Study Cohort, 9th Medical Research Conference, Faculty of Medicine, The University of Hong Kong 2004.

Cheung B.M.Y., Man Y.B., Wat N.M.S., Lo L.F., Chau F.Y., Lam T.H., Leung G.M., Tam S., Cheng C.H., Kumana C.R., Lau C.P. and Lam K.S.L., Prevalence of Hypertension in Hong Kong Cardiovascular Risk Factor Prevalence Study Cohort, J Hong Kong Coll Cardiol. 2004, 12: 33.

Cheung B.M.Y., Man Y.B., Lo L.F., Chau F.Y., Law C.Y., Lam K.S.L., Lam T.H., Wat N.M.S., Tam S., Cheng C.H., Kumana C.R. and Lau C.P., Relationship Between Hypertension and Obesity in Hong Kong, 3rd Asian-Pacific Congress on Hypertension, Singapore 2004.

Cheung B.M.Y., Lam T.H., Lam K.S.L., Tam S., Wat N.M.S., Man Y.B., Cheng C.H., Kumana C.R. and Lau C.P., The Hong Kong Cardiovascular Risk Factor Prevalence Survey Cohort - Results at 7 Years, J Hypertens. 2004, 22 (Suppl 2): S268-269.

Man Y.B., Cheung B.M.Y., Lam K.S.L., Wat N.M.S., Lo L.F., Chau F.Y., Law C.Y., Lam T.H., Leung G.M., Tam S., Cheng C.H., Kumana C.R. and Lau C.P., Prevalence of Hypertension in the Hong Kong Cardiovascular Risk Factor Prevalence Study Cohort, 9th Medical Research Conference, Faculty of Medicine, The University of Hong Kong. 2004.

Xu J., Dan Q., Chan V.N.Y., Wat N.M.S., Tam S., Tiu S.C., Lee K.F., Siu S.C., Tsang M.W., Fung L.M., Chan K.W. and Lam K.S.L., Genetic and Clinical Characteristics of Maturity-onset Diabetes of the Young in Chinese Patients, European Journal of Human Genetics. 2005, 13: 422-427.

Researcher : Tang JCO

List of Research Outputs

Fatima S., Hui K.S., Wong M.M., Tang W.K., Chui C.H., Wong J., Law S.Y.K., Tsao G.S.W., Lam K.Y., Srivastava G., Ho K.P. and Tang J.C.O., Study of transforming capacity of two novel genes JS-1 and JS-2 in chromosome 5p and their overexpression in human esophageal squamous cell carcinoma, 96th Annual Meeting of the American Association for Cancer Research, Anaheim, California, USA, 16-20 April. 2005.

Yang L., Leung A.C.C., Ko J.M.Y., Lo P.H.Y., Tang J.C.O., Srivastava G., Oshimura M., Stanbridge E.J., Daigo Y., Nakamura Y., Tang C.M.C., Lau K.W., Law S.Y.K. and Lung M.L., Tumor suppressive role of a 2.4 Mb 9q33-q34 critical region and DEC1 in esophageal squamous cell carcinoma, Oncogene. 2005, 24(4): 697-705.

Yang L.C., Tang J.C.O., Srivastava G., Stanbridge E.J. and Lung M.L., Functional investigation of tumor suppressive role of chromosome 9 in esophageal squamous cell carcinoma, Journal of Clinical Oncology. 2004, 22(14): 852S-852S 9571 Supp. S.

Researcher : To KW

List of Research Outputs

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Ma L., Chan K.W., Trendell-Smith N.J., Lo C.K., To K.W. and Huang F., Necrotic cells induce systemic autoimmune disease in vivo by activation of dendritic cells, 62th Annual Meeting, American Academy of Allergy, Asthma & Immunology (AAAAI), San Antonio, FL, USA, 3-7 March 2005.

To K.W., Chiang A.K.S. and Huang F., Tumor derived immuno-suppressive molecules on dendritic cells (DC) functions and the implications in DC-based vaccine, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Researcher : Trendell-Smith NJ

List of Research Outputs

Hon C., Ho S.L., Ma E.S.K., Trendell-Smith N.J. and Au W.Y., High-grade lymphoma after azathioprine treatment for Vogt-Kaganayi-Harada syndrome, Leukemia & Lymphoma . 2005, 46: 289-292.

Huang F., Ma L., Wu A.Y.Y., Tian L., Chan K.W., Trendell-Smith N.J., Lam A.C., Chan A.K.L., Lo C.K., Chik S.C.C., Ko K.H., To K.W., Kam S.K., Fong L.P., Li X.S., Leung S.Y., Ng M.H., Stott D.I., Liew F.Y. and MacPherson G.G., Systemic autoimmune disease induced by dendritic cells that have captured necrotic but not apoptotic dying cells, Proceedings of the 12th International Congress of Immunology and the 4th Annual Conference of FOCIS, Montreal, Canada, July 18-23, 2004. 2004.

Ma L., Chan K.W., Trendell-Smith N.J., Lo C.K., To K.W. and Huang F., Necrotic cells induce systemic autoimmune disease in vivo by activation of dendritic cells, 62th Annual Meeting, American Academy of Allergy, Asthma & Immunology (AAAAI), San Antonio, FL, USA, 3-7 March 2005.

Yeung C.K., Ma S.Y., Chan H.H.L., Trendell-Smith N.J. and Au W.Y., Primary CD30+ve Cutaneous T-cell Lymphoma Associated with Chronic Burn Injury in a Patient with Longstanding Psoriasis, American Journal of Dermatopathology. 2004, 26(5): 394-396.

Researcher : Tsun OKL

List of Research Outputs

Cheung A.N.Y., Chiu P.M., Tsun O.K.L., Khoo U.S., Leung B.S.Y. and Ngan H.Y.S., Chromosome in situ hybridisation, Ki-67, and telomerase immunocytochemistry in liquid based cervical cytology, Journal of Clinical Pathology. 2004, 57: 721-727.

Researcher : Wan TSK

List of Research Outputs

Au W.Y., Cheng V.C.C., Wan T.S.K. and Ma E.S.K., Myelodysplasia masquerading as parvovirus-related red cell aplasia with giant pronormoblasts, Annals of Hematology. 2004, 83(10): 670-1.

Au W.Y., Srivastava G., Wong K.Y., Chung L.P., Ma E.S.K., Wan T.S.K. and Kwong Y.L., Transformation of diffuse large B-cell lymphoma into pre-B acute lymphoblastic leukemia: clinicopathologic features and clonal relationship, Human Pathology. 2004, 35(7): 900-903.

Chung C.M., Man W.Y.C., Jin Y., Jin C., Guan X.Y., Wang Q., Wan T.S.K., Cheung A. and Tsao G.S.W., Amplification and overexpression of Aurora kinase A (AURKA) in immortalized human ovarian epithelial (HOSE) cells, Molecular Carcinogenesis. 2005, 43(3): 165-174.

Ma E.S.K., Wan T.S.K. and Chan L.C., FISHing - Current status and future prospects for improved management of leukemia, Cancer Reviews: Asia-Pacific. 2004, 2(2): 131-141.

Wan T.S.K., Ma E.S.K., Chan G.C.F. and Chan L.C., Investigation of MYCN status in neuroblastoma by fluorescence in situ hybridization, International Journal of Molecular Medicine. 2004, 14: 981-987.

Wan T.S.K., Ma E.S.K., Chow E.Y.D., Li Y.H., Lin S.Y. and Chan L.C., Pathogenesis of jumping translocations: a molecular cytogenetics study, Leukemia Research. 2004, 28(10): 1075-1079.

Researcher : Wang X

Project Title:	Downregulation of MAD2 expression and its significance in chemodrug sensitization in nasopharyngeal carcinoma cells
Investigator(s):	Wang X, Nicholls JM, Tsao GSW, Jin D
Department:	Anatomy
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

To confirm that MAD2 expression is downregulated in clinical samples of NPC; to determine the mechanism by which MAD2 is downregulated in NPC; to establish whether upregulation of MAD2 expression can sensitize NPC cells to chemotherapy.

Project Title:	Downregulation of MAD2 expression and its significance in chemodrug sensitization in nasopharyngeal carcinoma cells
Investigator(s):	Wang X, Nicholls JM, Tsao GSW, Jin D
Department:	Anatomy
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

Project Title:	The role fo MAD2 in overcoming cisplatin resistance in testicular germ cell tumors
Investigator(s):	Wang X, Jin D
Department:	Anatomy
Source(s) of Funding:	Lance Armstrong Foundation - General Award
Start Date:	10/2003

Abstract:

To investigate the association between decreased MAD2 protein expression and cisplatin resistance in TGCT cells; to study if exogenous expression of the MAD2 gene in TGCT cells can lead to chemosensitization to cisplatin in TGCT cells; to demonstrate that downregulation of MAD2 results in resistance ot cisplatin in TGCT cells; to characterize the role of MAD2 in cisplatin-induced cell death inTGCT cells; to identify binding partners of MAD2 in response to cisplatin-induced DNA damage.

Project Title:	The role of Id-1 in chemodrug resistance in nasopharyngeal carcinoma cells
Investigator(s):	Wang X, Wong YC
Department:	Anatomy
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	02/2004
Completion Date:	12/2004

Abstract:

To study if ectopic Id-1 expression could lead to resistance to chemodrugs, 5-FU, taxol and cisplatin; to examine if the protective role of Id-1 is through inhibition of chemodrug-induced apoptosis; to investigate if MAPK and NF_[kappa]B pathways are involved in the Id-1 induced chemo-protection in NPC cells.

Project Title:	Significance of MAD2 expression to chromosomal instability in prostate cancer
Investigator(s):	Wang X, Wong Y C, Jin D Y
Department:	Anatomy
Source(s) of Funding:	Association for International Cancer Research - General Award
Start Date:	04/2004

Abstract:

To correlate MAD2 expression with genomic instability in prostate cancer specimens; to show that MAD2 expression is essential for a functional mitotic checkpoint in prostate cancer cells; to demonstrate that downregulation of MAD2 leads to mitotic checkpoint defect and increased CIN in prostate cancer cells.• To investigate if promoter hypermethylation contributes to decreased MAD2 expression in prostate cancer

Project Title:	Upregulation of TWIST and its implication as a novel therapeutic target in the treatment of prostate cancer
Investigator(s):	Wang X
Department:	Anatomy
Source(s) of Funding:	Incentive Award for RGC CERG Fundable But Not Funded Projects
Start Date:	07/2004

Abstract:

Refer to hard copy

Project Title:	Role of ld-1 in the proliferation of ovarian cancer cells
Investigator(s):	Wang X, Wong YC
Department:	Anatomy
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	03/2005

Abstract:

refer hard copy proposal

Researcher : Wong CM

List of Research Outputs

Chin K.T., Zhou H., Wong C.M., Lee M.F., Chan C.P., Qiang B.Q., Yuan J.G., Ng I.O.L. and Jin D., The liver-enriched transcription factor CREB-H is a growth suppressor protein underexpressed in hepatocellular carcinoma, Nucleic Acids Research. 2005, 33(6): 1859-1873.

Ching Y.P., Wong C.M., Jin D. and Ng I.O.L., Identification of a novel RHO GTPASE activating protein called DLC2, involved in hepatocellular carcinoma, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004 . 2004.

Leung T.H.Y., Ching Y.P., Wong C.M., Ng D.C.H., Jin D. and Ng I.O.L., Identification of multiple isoforms of DLC2, a novel tumor suppressor gene, and their functional characterization in hepatocellular carcinoma, 4th International Meeting of Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, December 2004 (Young Investigator Award). 2004.

Wong C.M., Ching Y.P. and Ng I.O.L., Genome-wide methylation screening in search for novel tumor suppressor genes in liver cancer, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Frequent down-regulation of HDPR1, a novel inhibitor of WNT/beta-catenin signaling, in hepatocellular carcinoma (Abstract), The 6th Annual Scientific Meeting, Centre for the Study of Liver Disease, The University of Hong Kong, Hong Kong, 8 January 2005.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of WNT/b-catenin signaling, is down-regulated in hepatocellular carcinoma: involvement of promoter hypermethylation, AACR Special Conference in Cancer Research “Chromatin, chromosomes and cancer epigenetics”, Hawaii, USA, November 2004.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., HDPR1, a novel inhibitor of the WNT/beta-catenin signaling, is frequently downregulated in hepatocellular carcinoma: involvement of methylation-mediated gene silencing, Oncogene. 2005, 24(9): 1607-1614.

Yau T.O., Chan C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), Asian Journal of Surgery. 2005, 28(Suppl 2): S84.

Yau T.O., Chan P.C.Y., Chan K.L., Lee M.F., Wong C.M., Fan S.T. and Ng I.O.L., Under-expression of HDPR1, a novel inhibitor of WNT/b-catenin signaling pathway, in hepatocellular carcinoma (Abstract), The 4th International Meeting on Hepatocellular Carcinoma: Eastern and Western Experiences, Hong Kong, 14-16 December 2004.

Researcher : Wong CW

List of Research Outputs

Wong C.W., Fan Y.S., Chan T.L., Chan A.S.W., Ho L.C., Ma T.K.F., The Cancer Genome Project , Yuen S.T. and Leung S.Y., BRAF and NRAS mutations are uncommon in melanomas arising in diverse internal organs, Journal of Clinical Pathology. 2005, 58: 640-644.

Researcher : Wong KY

List of Research Outputs

Chim J.C.S., Wong K.Y., Loong F. and Srivastava G., Absence of ATM hypermethylation in mantle cell and follicular lymphoma, Leukemia. 2005, 19(5): 880-2.

Lo C.C., Wong K.Y. and Chan K.W., BRAF mutation in papillary thyroid carcinoma, 13th Annual Scientific Meeting of the Hong Kong Division of the International Academy of Pathology, Hong Kong, 12-14 November 2004.

Lo C.C., Wong K.Y. and Chan K.W., Ras mutation in papillary thyroid carcinoma, 13th Annual Scientific Meeting of the Hong Kong Division of the International Academy of Pathology, Hong Kong, 12-14 November 2004.

Researcher : Wong MP

Project Title:	Analysis of genetic changes in non-small cell lung cancers from non-smokers in Hong Kong
Investigator(s):	Wong MP, Chung LP, Lam WK, Chiu S.W.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2002

Abstract:

To identify loci of significant DNA gain, implicating the presence of potential oncogences, or loss, implicating the presence of potential tumour suppressor genes (TSG) in NSCLC from non-smokers; to identify genes or DNA sequences that show up -or down- regulated expression levels in NSCLC of non-smokers compared to normal lung; to identify and analyse candidate oncogenes or TSG for activating mutations or inactivating genetic or epigenetic alterations.

Project Title:	Lymphocytic BPDE-DNA adduct level and its relation with genomic aberrations in primary non-small cell lung carcinomas from smokers and non-smokers
Investigator(s):	Wong MP, Chung LP, Lu L
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2002
Completion Date:	10/2004

Abstract:

To study the peripheral blood lymphocytic BPDE-DNA adduct level and its relation with genomic aberrations in primary non-small cell lung carcinomas of Hong Kong.

Project Title:	Analysis of the pathology and pathogenesis in the lungs of patients suffering from the Severe Acute Respiratory Syndrome (SARS)
Investigator(s):	Wong MP, Nicholls JM, Chung LP, Beh SL, Lee K.C., Pang S.W., Leung C.Y.
Department:	Pathology
Source(s) of Funding:	VCO SARS Research Fund
Start Date:	07/2003

Abstract:

To evaluate the pathological changes in the lung in patients suffering from SARS.

Project Title:	Identification of candidate cancer genes in regions of frequent chromosomal aberration in non-small cell lung cancers
Investigator(s):	Wong MP, Chung LP, Lam WK, Chiu S.W.
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	09/2003

Abstract:

To define critical regions of imbalance in frequently altered chromosomal loci identified in local samples of non-small cell lung cancers (NSCLC) by microstatellite analysis; to identify target genes in the analyzed regions by comparison with genes listed in databases of the Human Genome Resources(NCBI); to study the structure and expression of the targeted genes in NSCLC of smokers and non-smokers, so as to verify their involvement and to look for genetic or epigenetic alterations.

Project Title:	Identification of candidate cancer genes in regions of frequent chromosomal aberration in non-small cell lung cancers
Investigator(s):	Wong MP, Chung LP, Lam WK, Chiu S.W.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	09/2003

Abstract:

Project Title:	Analysis of allelic imbalance at 16q24 and WW domain-containing oxidoreductase (WWOX) alterations in primary non-small cell lung cancer in smokers and non-smokers
Investigator(s):	Wong MP, Chung LP
Department:	Pathology
Source(s) of Funding:	Small Project Funding
Start Date:	11/2003
Completion Date:	10/2004

Abstract:

To analysis allelic imbalance at 16q24; to analysis WWOX genetic and epigenetic alterations in lung cancers.

Project Title:	Differentially expressed genes in lung cancer
Investigator(s):	Wong MP, Chung LP, Lam WK, Lam E.C.L., Chiu S.W.
Department:	Pathology
Source(s) of Funding:	Competitive Earmarked Research Grants (CERG)
Start Date:	01/2005

Abstract:

To verify whether molecular targets identified through expression profile microarray screening procesures truly show aberrant expressions in extended samples of lung cancers; to evaluate whether the genes encoding these verified molecular targets show alterations or mutations, so that their primary roles in carcinogenesis are supported at the genomic level.

Project Title:	Differentially expressed genes in lung cancer
Investigator(s):	Wong MP
Department:	Pathology
Source(s) of Funding:	Merit Award for RGC CERG Funded Projects
Start Date:	01/2005

Abstract:

Refer to hard copy

List of Research Outputs

Chan M.M.W., Lam W.K., Mak J.C.W., Ho S.P., Chan H.W., Tsang K.W.T., Ip M.S.M., Ho J.C.M., Wong M.P. and Tan-Un K., Polymorphisms of CYP and GST genes, passive smoke and lung cancer risk in Hong Kong Chinese, European Respiratory Journal. 2004, 24: Suppl 48: 457s.

Chen L., Wong M.P., Cheung L.K., Samaranayake L.P., Baum L. and Samman N., Frequent allelic loss of 21q11.1-q21.1 region in advanced stage oral squamous cell carcinoma, Cancer Genetics and Cytogenetics. 2005, 159: 37-43.

Girard L., Lam C.L., Shigematsu H., Wong M.P., Peyton M., Sheridan S., Beer D.G., Gazdar A.F. and Minna J.D., Gene profiling of lung cancers with EGFR mutations, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Ho J.C.M., Lam W.K., Wong M.P., Wong M.K., Ooi C.G.C., Ip M.S.M., Chan M.M.W. and Tsang K.W.T., Lymphoepithelioma-like carcinoma of the lung : experience with ten cases, International Journal of Tuberculosis and Lung Disease. 2004, 8(7): 890-895.

Ho J.C.M., Ho S.P., Chan M.M.W., Mak J.C.W., Ip M.S.M., Ko K.W., Yan C.P.K., Wong M.P., Tsang K.W.T. and Lam W.K., Non-small cell lung cancer in Chinese is associated with disturbance in systemic antioxidant profiles, 9th Congress of Asian Pacific Society of Respirology, Hong Kong, December, 2004. Respirology. 2004, 9 (Supp): A114.

Ho S.P., Chan M.M.W., Wong M.P., Tsang K.W.T., Ip M.S.M., Lam W.K. and Mak J.C.W., Transforming growth factor (TGF)-beta 1 gene common polymorphisms and plasma TGF-beta 1 levels in patients with lung cancer, 10th Medical Research Conference, Department of Medicine, The University of Hong Kong, February 2005. Abstract Book: RM 60.

Lam B., Tam C.M., Lam S.Y., Wong M.P., Ooi C.G.C., Fung S.L., Ip M.S.M. and Lam W.K., Detection of early lung cancer in high risk population : a prospective study, 9th Congress of Asian Pacific Society of Respirology, Hong Kong, December 2004. Respirology. 2004, 9 (Supp): A122.

Lam C.L., Wong M.P., Girard L., Chung L.P., Chau W.S., Chiu S.W., Lam W.K. and Minna J.D., Gene expression profiling in lung adenocarcinomas reveals molecular signatures of potential biological significance , Journal of The Japanese Respiratory Society. 2005, 43 (Suppl): 115 (EO6-3).

Lam D.C.L., Wong M.P., Girard L., Shigematsu H., Chung L.P., Gazdar A.F., Chiu S.W., Suen W.S., Lam W.K. and Minna J.D., Expression profiling in lung adenocarcinoma with or without Epidermal Growth Factor Receptor (EGFR) gene mutation at exons 18-21 reveals expression signatures related to the EGFR pathway, 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, P.O.CB 39: 888/B#9.

Lam D.C.L., Wong M.P., Girard L., Chung L.P., Chau W.S., Chiu S.W., Lam W.K. and Minna J.D., Gene expression profiling in lung adenocarcinomas reveals molecular signatures of potential biological significance, Respirology. 2004, 9 (Suppl): A121 (236).

Tam I.Y.S., Wong M.P., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., EGFR mutations are prevalent and independent from K-RAS mutations in lung adenocarcinomas from non-smokers, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Tam I.Y.S., Wong M.P., Suen W.S., Wang E., Lam W.K., Chiu S.W., Gazdar A., Minna J.D. and Chung L.P., EGFR mutations are prevalent and independent from k-RAS mutations in lung adenocarcinomas from non-smokers, 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, M.S.CL00.01: 1666.

Tsang K.W.T., Tipoe G.L., Mak J.C.W., Sun J.Z., Wong M.P., Leung R.C.M., Tan K.C.B., MedStat C.K.M., Ho J.C.M., Ho P.L., Rutman A. and Lam W.K., Ciliary central microtubular orientation is of no clinical significance in bronchiectasis, Respiratory Medicine. 2005, 99: 290-297.

Wong M.P., Lam D.C.L., Yap D.Y.L., Girard L., Tam I.Y.S., Chung L.P., Chiu S.W., Lam W.K., Danchin A. and Minna J.D., Identification of discriminating gene expression of EBV-associated primary lymphoepitheioma-like carcinoma of lung by oligonucleotide microarray analysis , 96th Annual Meeting of the American Association for Cancer Research, Anaheim, CA, USA, April. 2005, P.O.CB19: 75/B#3.

Wong M.P., Molecular genetics of non-small cell lung cancer in non-smokers, The Croucher Advanced Study Institute on “Molecular Genetics Cell Signaling in Cancers” Hong Kong, 17-21 January 2005.

Wong M.P., Updates on pathological classification of lung cancer, 9th Asian Pacific Society of Respirology Congress, 10 December 2004, Hong Kong. 2004.

Yap Y.L., Lam C.L., Girard L., Zhang X., Hernandez D., Gras R., Wang E., Chiu S. .W., Chung L.P., Lam W.K., Smith D.K., Minna J. .D., Danchin A. and Wong M.P., Conserved transcription factor binding sites of cancer markers derived from primary lung adenocarcinoma microarrays, Nucleic Acids Research. Oxford University Press 2005, 2004, 33 No. 8: 2764.

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., High-resolution analysis of DNA copy number alterations and expression profiling by microarray technology in primary lung cancer cell lines, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Researcher : Xue W

List of Research Outputs

Chan Q.K.Y., Khoo U.S., Chan Y.K., Ngan H.Y.S., Li S.S., Chiu P.M., Man M.L.S., Ip P.P.C., Xue W. and Cheung A.N.Y., Promoter methylation and differential expression of p-Class glutathione S-transferase in endometrial carcinoma, The Journal of Molecular Diagnostics. 2005, 7(1): 8-16.

Chan Q.K.Y., Khoo U.S., Ngan H.Y.S., Yang C., Xue W., Chan Y.K., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Single nucleotide polymorphism of Pi-Class glutathione S-transferase and susceptibility to endometrial carcinoma, Clinical Cancer Research. 2005, 11(8): 2981-2985.

Fong P.Y., Xue W., Ngan H.Y.S., Chan Y.K., Khoo U.S., Tsao G.S.W., Chiu P.M., Man M.L.S. and Cheung A.N.Y., Mcl-1 expression in gestational trophoblastic disease correlates with clinical outcome, Cancer. 2004, 103(2): 268-276.

Li S.S., Xue W., Khoo U.S., Ngan H.Y.S., Chan Y.K., Tam I.Y.S., Chiu P.M., Ip P.P.C., Tam K.F. and Cheung A.N.Y., Replicative MCM7 protein as a proliferation marker in endometrial carcinoma: A tissue microarray and clinicopathological analysis, Histopathology. 2005, 46(3): 307-313.

Shen D.H., Chan Y.K., Khoo U.S., Ngan H.Y.S., Xue W., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Epigenetic and genetic alterations of p33ING1b in ovarian cancer., In: Shen DH*, Chan YK*, Khoo US, Ngan HY, Xue WC, Chiu PM, Ip PP, Cheung AN. , Carcinogenesis . 2005, 26: 855-63.

Xue W., Chan Y.K., Feng H., Chiu P.M., Ngan H.Y.S., Tsao G.S.W. and Cheung A.N.Y., Promoter hypermethylation of multiple genes in hydatidiform mole and choriocarcinoma, The Journal of Molecular Diagnostics. 2004, 6(4): 326-334.

Researcher : Xue W

List of Research Outputs

Researcher : Yam JWP

Project Title:	Characterization of tensin2, the binding partner of DLC1 tumor suppressor in liver cancer
Investigator(s):	Yam JWP, Ng IOL
Department:	Pathology
Source(s) of Funding:	Seed Funding Programme for Basic Research
Start Date:	12/2004

Abstract:

refer hard copy proposal

List of Research Outputs

Cheung N., So C.W., Yam J.W.P., So C.K.C., Poon R.Y.C., Jin D. and Chan L.C., Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukemia, Biochemical Journal. 2004, 383(Pt 1): 27-35.

Yam J.W.P., Jin D. and Chan L.C., Identification and characterization of EBP, a Novel EEN binding protein that inhibits ras signaling and is recruited into the nucleus by the MLL-EEN fusion protein, 10th SCBA International Symposium, Beijing, China, 18-23 July 2004.

Researcher : Yang C

List of Research Outputs

Chan Q.K.Y., Khoo U.S., Ngan H.Y.S., Yang C., Xue W., Chan Y.K., Chiu P.M., Ip P.P.C. and Cheung A.N.Y., Single nucleotide polymorphism of Pi-Class glutathione S-transferase and susceptibility to endometrial carcinoma, Clinical Cancer Research. 2005, 11(8): 2981-2985.

Yang C. and Huang F., Regulation of autoimmune responses by dendritic cells and T regulatory cells in Systemic Lupus Erythematosus, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

Yang C., Single nucleotide polymorphism in the coding sequence of follicle stimulating hormone receptor and susceptibility to ovarian and endometrial cancer. 2004.

Researcher : Yang C

List of Research Outputs

Yang C., Single nucleotide polymorphism in the coding sequence of follicle stimulating hormone receptor and susceptibility to ovarian and endometrial cancer. 2004.

Researcher : Yang C

List of Research Outputs

Yang C., Single nucleotide polymorphism in the coding sequence of follicle stimulating hormone receptor and susceptibility to ovarian and endometrial cancer. 2004.

Researcher : Yip SP

List of Research Outputs

Liu W., Chan Y.K., Chan S.Y., Yip S.P., Cheung A.N.Y., Chua D.T.T., Ngan H.Y.S. and Khoo U.S., BRCA1 gene promoter polymorphisms associated with breast cancer risk, 96th Annual Meeting 2005, American Association for Cancer Research, Anaheim, Orange Country, California, USA, April 16-20, 2005.

Researcher : Yuen ST

List of Research Outputs

He Q., Cheung Y.H., Leung S.Y., Yuen S.T., Chu K.M. and Chiu J., Diverse proteomic alterations in gastric adenocarcinoma, Proteomics. 2004, 4(10): 3276-3287.

Ho J.W.C., Ho M.Y., Wong K., Chan C.L.W., Chan E. and Yuen S.T., Psychological profile of colorectal cancer genetic testing recipitnes in Hong Kong, Familial Cancer. 2005, 4 (Supp 1): 50 (F33).

Ho J.W.C., Ho M.Y., Wong K., Chan C.L.W., Chan E. and Yuen S.T., Psychological profile of colorectal cancer genetic testing recipitnes in Hong Kong, First Conference of InSiGHT, Newcastle upon Tyne, UK, 14-17 June 2005.

Ko S., Chu K.M., Luk J.M.C., Wong W.Y., Yuen S.T., Leung S.Y. and Wong J., CDX2 co-localizes with liver-intestine cadherin in intestinal metaplasia and adenocarcinoma of stomach, Journal of Pathology . 2005, 205: 615-622.

Leung P.W., Lee C.C., Hung S.F., Ho T.P., Tang C.P., Kwong S.L., Leung S.Y., Yuen S.T., Mak-Lieh F., Oosterlaan J., Grady D., Harxhi A., Ding Y.C., Chi H.C., Flodman P., Schuck S., Spence M.A., Moyzis R. and Swanson J., Dopamine receptor D4 (DRD4) gene in Han Chinese children with attention-deficit/hyperactivity disorder (ADHD): increased prevalence of the 2-repeat allele, American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics. 2005, 133B(1): 54-56.

Leung S.Y., Yuen S.T., Chu K.M., Mathy J.A., Li R., Chan A.S.Y., Law S.Y.K., Wong J., Chen X. and So S., Expression profiling identifies chemokine (C-C motif) ligand 18 as an independent prognostic indicator in gastric cancer, Gastroenterology. 2004, 127(2): 457-469.

Li V.S.W., Wong C.W., Chan T.L., Chan A.S.W., Zhao W., Chu K.M., Chen X., Yuen S.T., Leung S.Y. and So S., Mutations of PIK3CA in gastric adenocarcinoma, BioMed Central Cancer. 2005, 5: 29.

Stephens P., Edkins S., Davies H., Greenman C., Cox C., Hunter C., Bignell G., Teague J., Smith R., Stevens C., O'Meara S., Parker A., Tarpey P., Avis T., Barthorpe A., Brackenbury L., Buck G., Butler A., Clements J., Cole J., Dicks E., Edwards K., Forbes S., Gorton M., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jones D., Kosmidou V., Laman R., Lugg R., Menzies A., Perry J., Petty R., Raine K., Shepherd R., Small A., Solomon H., Stephens Y., Tofts C., Varian J., Webb A., West S., Widaa S., Yates A., Brasseur F., Cooper C.S., Flanagan A.M., Green A., Knowles M., Leung S.Y., Looijenga L.H., Malkowicz B., Pierotti M.A., Teh B., Yuen S.T., Nicholson A.G., Lakhani S., Easton D.F., Weber B.L., Stratton M.R., Futreal P.A. and Wooster R., A screen of the complete protein kinase gene family identifies diverse patterns of somatic mutations in human breast cancer, Nature Genetics. 2005, 37(6): 590-592.

Stephens P., Hunter C., Bignell G., Edkins S., Davies H., Teague J., Stevens C., O'Meara S., Smith R., Parker A., Barthorpe A., Blow M., Brackenbury L., Butler A., Clarke O., Cole J., Dicks E., Dike A., Drozd A., Edwards K., Forbes S., Foster R., Gray K., Greenman C., Halliday K., Hills K., Kosmidou V., Lugg R., Menzies A., Perry J., Petty R., Raine K., Ratford L., Shepherd R., Small A., Stephens Y., Tofts C., Varian J., West S., Widaa S., Yates A., Brasseur F., Cooper C.S., Flanagan A.M., Knowles M., Leung S.Y., Louis D.N., Looijenga L.H., Malkowicz B., Pierotti M.A., Teh B., Chenevix-Trench G., Weber B.L., Yuen S.T., Harris G., Goldstraw P., Nicholson A.G., Futreal P.A., Wooster R. and Stratton M.R., Lung cancer: intragenic ERBB2 kinase mutations in tumours, Nature. 2004, 431(7008): 525-526.

Subramanian S., West R.B., Corless C.L., Ou W., Rubin B.P., Chu K.M., Leung S.Y., Yuen S.T., Zhu S., Hernandez-Boussard T., Montgomery K., Nielsen T.O., Patel R.M., Goldblum J.R., Heinrich M.C., Fletcher J.A. and van de Rijn M.V.D., Gastrointestinal stromal tumors (GISTs) with KIT and PDGFRA mutations have distinct gene expression profiles, Oncogene. 2004, 2004: 7780-7790.

Wong C.W., Fan Y.S., Chan T.L., Chan A.S.W., Ho L.C., Ma T.K.F., The Cancer Genome Project , Yuen S.T. and Leung S.Y., BRAF and NRAS mutations are uncommon in melanomas arising in diverse internal organs, Journal of Clinical Pathology. 2005, 58: 640-644.

Xia H.H.X., Yang Y., Lam S.K., Wong R.W.M., Leung S.Y., Yuen S.T., Elia G., Wright N.A. and Wong B.C.Y., Aberrant Epithelial Expression of Trefoil Family Factor 2 and Mucin 6 in Helicobacter pylori infected gastric antrum, incisura, and body and its association with antralisation, Journal of clinical pathology. 2004, 57: 861-866.

Xia H.H.X., Lam S.K., Huang X.R., Wong R.W.M., Leung S.Y., Yuen S.T., Lan H.Y. and Wong B.C.Y., Helicobacter pylori Infection is Associated with Increased Expression of Macrophage Migratory Inhibitory Factor - by Epithelial Cells, T Cells, and Macrophages - in Gastric Mucosa, The Journal of Infectious Diseases. 2004, 190: 293-302.

Researcher : Zhang M

List of Research Outputs

Lu L., Zhang M., Cheung C.T., Wong C., Ko K.H., Tsang S.L., Chan L.C. and Sham M.H., Hoxb3 deficiency impairs B lymphopoiesis, 12th International Conference for Immunology, Montreal, Canada, July 18-23, 2004. 2004.

Lu L., Lam Q.L.K., Zhang M., Lo K.C., Ko K.H., Osmond D.G., Wu G.E. and Rottapel R., Novel function of c-Abl in regulating precursor B cell development, The 3rd Congress of the Federation of Immunology Societies of Asia-Oceania, Hangzhou, China, April 18-22, 2005.

Zhang M., Ko K.H., Sham M.H. and Lu L., Novel function of HOXB3 gene in regulation B lymphopoiesis , 9th research postgraduate symposium, HKU, December 4, 2004.

Zhang M., Ko K.H., Lam L.K.Q., Lo K.C., Xu D.J.L., Shen L., Zheng B., Srivastava G. and Lu L., Novel function of TNF cytokines in regulating bone marrow B cell survival, Cellular & Molecular Immunology. 2004, 1(6): 447-453.

Researcher : Zhang M

List of Research Outputs

Zhang M., Ko K.H., Sham M.H. and Lu L., Novel function of HOXB3 gene in regulation B lymphopoiesis , 9th research postgraduate symposium, HKU, December 4, 2004.

Researcher : Zhao W

List of Research Outputs

Li V.S.W., Wong C.W., Chan T.L., Chan A.S.W., Zhao W., Chu K.M., Chen X., Yuen S.T., Leung S.Y. and So S., Mutations of PIK3CA in gastric adenocarcinoma, BioMed Central Cancer. 2005, 5: 29.

Researcher : Zhu H

List of Research Outputs

Zhu H., Wong M.P., Lam C.L., Cai W.W., Chau W.S., Wang E., Lam W.K., Chiu S.W. and Chung L.P., High-resolution analysis of DNA copy number alterations and expression profiling by microarray technology in primary lung cancer cell lines, 9th Research Postgraduate Symposium, The University of Hong Kong, Hong Kong, 4 December 2004.

-- End of Listing --